LOCUS       DAZUJO010000001        97310 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000001 DAZUJO010000000
VERSION     DAZUJO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 97310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.001"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..1251
                     /locus_tag="ACG8OL_000001"
     CDS             127..1251
                     /locus_tag="ACG8OL_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4816026.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1478..2395
                     /locus_tag="ACG8OL_000002"
     CDS             1478..2395
                     /locus_tag="ACG8OL_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4816027.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2388..3221
                     /locus_tag="ACG8OL_000003"
     CDS             2388..3221
                     /locus_tag="ACG8OL_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4816028.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3304..4422
                     /locus_tag="ACG8OL_000004"
     CDS             3304..4422
                     /locus_tag="ACG8OL_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4816029.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4443..6314
                     /locus_tag="ACG8OL_000005"
     CDS             4443..6314
                     /locus_tag="ACG8OL_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS4816030.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            6308..7531
                     /locus_tag="ACG8OL_000006"
     CDS             6308..7531
                     /locus_tag="ACG8OL_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4816031.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7617..9551)
                     /locus_tag="ACG8OL_000007"
     CDS             complement(7617..9551)
                     /locus_tag="ACG8OL_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4816032.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            9777..10433
                     /locus_tag="ACG8OL_000008"
     CDS             9777..10433
                     /locus_tag="ACG8OL_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4816033.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(10507..10860)
                     /locus_tag="ACG8OL_000009"
     CDS             complement(10507..10860)
                     /locus_tag="ACG8OL_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4816034.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            11052..11981
                     /locus_tag="ACG8OL_000010"
     CDS             11052..11981
                     /locus_tag="ACG8OL_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS4816035.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11992..12828
                     /locus_tag="ACG8OL_000011"
     CDS             11992..12828
                     /locus_tag="ACG8OL_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS4816036.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            13076..13852
                     /locus_tag="ACG8OL_000012"
     CDS             13076..13852
                     /locus_tag="ACG8OL_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4816037.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            14028..14771
                     /locus_tag="ACG8OL_000013"
     CDS             14028..14771
                     /locus_tag="ACG8OL_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4816038.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            14764..16344
                     /locus_tag="ACG8OL_000014"
     CDS             14764..16344
                     /locus_tag="ACG8OL_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS4816039.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(16595..17074)
                     /locus_tag="ACG8OL_000015"
     CDS             complement(16595..17074)
                     /locus_tag="ACG8OL_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4816040.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(17090..17842)
                     /locus_tag="ACG8OL_000016"
     CDS             complement(17090..17842)
                     /locus_tag="ACG8OL_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS4816041.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNNLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17959..18029
                     /locus_tag="ACG8OL_000017"
     tRNA            17959..18029
                     /locus_tag="ACG8OL_000017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17991..17993,aa:Gly,seq:ccc)
     gene            18390..19115
                     /locus_tag="ACG8OL_000018"
     CDS             18390..19115
                     /locus_tag="ACG8OL_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS4816042.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            19370..19636
                     /locus_tag="ACG8OL_000019"
     CDS             19370..19636
                     /locus_tag="ACG8OL_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS4816043.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            19633..20064
                     /gene="ruvX"
                     /locus_tag="ACG8OL_000020"
     CDS             19633..20064
                     /gene="ruvX"
                     /locus_tag="ACG8OL_000020"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS4816044.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            20089..20394
                     /locus_tag="ACG8OL_000021"
     CDS             20089..20394
                     /locus_tag="ACG8OL_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS4816045.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20475..21620)
                     /locus_tag="ACG8OL_000022"
     CDS             complement(20475..21620)
                     /locus_tag="ACG8OL_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4816046.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21681..22328)
                     /locus_tag="ACG8OL_000023"
     CDS             complement(21681..22328)
                     /locus_tag="ACG8OL_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4816047.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            22522..22815
                     /locus_tag="ACG8OL_000024"
     CDS             22522..22815
                     /locus_tag="ACG8OL_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816048.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            22859..23170
                     /locus_tag="ACG8OL_000025"
     CDS             22859..23170
                     /locus_tag="ACG8OL_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816049.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            23167..23791
                     /locus_tag="ACG8OL_000026"
                     /pseudo
     CDS             23167..23791
                     /locus_tag="ACG8OL_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            23932..24090
                     /locus_tag="ACG8OL_000027"
     CDS             23932..24090
                     /locus_tag="ACG8OL_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816050.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            24091..24453
                     /locus_tag="ACG8OL_000028"
     CDS             24091..24453
                     /locus_tag="ACG8OL_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816051.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            24490..25338
                     /locus_tag="ACG8OL_000029"
     CDS             24490..25338
                     /locus_tag="ACG8OL_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS4816052.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            25328..26803
                     /locus_tag="ACG8OL_000030"
     CDS             25328..26803
                     /locus_tag="ACG8OL_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS4816053.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            27069..27476
                     /locus_tag="ACG8OL_000031"
     CDS             27069..27476
                     /locus_tag="ACG8OL_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS4816054.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            27479..27694
                     /locus_tag="ACG8OL_000032"
     CDS             27479..27694
                     /locus_tag="ACG8OL_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816055.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            27698..28078
                     /locus_tag="ACG8OL_000033"
     CDS             27698..28078
                     /locus_tag="ACG8OL_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS4816056.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            28212..28367
                     /locus_tag="ACG8OL_000034"
     CDS             28212..28367
                     /locus_tag="ACG8OL_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS4816057.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            28436..28909
                     /locus_tag="ACG8OL_000035"
     CDS             28436..28909
                     /locus_tag="ACG8OL_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS4816058.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            28906..30600
                     /locus_tag="ACG8OL_000036"
     CDS             28906..30600
                     /locus_tag="ACG8OL_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4816059.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            30566..30751
                     /locus_tag="ACG8OL_000037"
     CDS             30566..30751
                     /locus_tag="ACG8OL_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816060.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            30755..31930
                     /locus_tag="ACG8OL_000038"
     CDS             30755..31930
                     /locus_tag="ACG8OL_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4816061.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            31923..33446
                     /locus_tag="ACG8OL_000039"
     CDS             31923..33446
                     /locus_tag="ACG8OL_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4816062.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            33502..33786
                     /locus_tag="ACG8OL_000040"
     CDS             33502..33786
                     /locus_tag="ACG8OL_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4816063.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            33773..34108
                     /locus_tag="ACG8OL_000041"
     CDS             33773..34108
                     /locus_tag="ACG8OL_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4816064.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            34403..35029
                     /locus_tag="ACG8OL_000042"
     CDS             34403..35029
                     /locus_tag="ACG8OL_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816065.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            35771..36607
                     /locus_tag="ACG8OL_000043"
     CDS             35771..36607
                     /locus_tag="ACG8OL_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS4816066.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            36671..37102
                     /locus_tag="ACG8OL_000044"
     CDS             36671..37102
                     /locus_tag="ACG8OL_000044"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4816067.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(37164..38486)
                     /locus_tag="ACG8OL_000045"
     CDS             complement(37164..38486)
                     /locus_tag="ACG8OL_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS4816068.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(38640..40280)
                     /locus_tag="ACG8OL_000046"
     CDS             complement(38640..40280)
                     /locus_tag="ACG8OL_000046"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS4816069.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            40613..41083
                     /locus_tag="ACG8OL_000047"
     CDS             40613..41083
                     /locus_tag="ACG8OL_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS4816070.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            41305..42408
                     /locus_tag="ACG8OL_000048"
     CDS             41305..42408
                     /locus_tag="ACG8OL_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS4816071.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            42692..43372
                     /locus_tag="ACG8OL_000049"
     CDS             42692..43372
                     /locus_tag="ACG8OL_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4816072.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            43462..44496
                     /locus_tag="ACG8OL_000050"
     CDS             43462..44496
                     /locus_tag="ACG8OL_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4816073.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            44510..44845
                     /locus_tag="ACG8OL_000051"
     CDS             44510..44845
                     /locus_tag="ACG8OL_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816074.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            44829..47309
                     /locus_tag="ACG8OL_000052"
     CDS             44829..47309
                     /locus_tag="ACG8OL_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816075.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            47365..48924
                     /locus_tag="ACG8OL_000053"
     CDS             47365..48924
                     /locus_tag="ACG8OL_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816076.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            48921..50285
                     /locus_tag="ACG8OL_000054"
     CDS             48921..50285
                     /locus_tag="ACG8OL_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4816077.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            50456..51526
                     /locus_tag="ACG8OL_000055"
     CDS             50456..51526
                     /locus_tag="ACG8OL_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4816078.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            51541..52209
                     /locus_tag="ACG8OL_000056"
     CDS             51541..52209
                     /locus_tag="ACG8OL_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4816079.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            52229..52807
                     /locus_tag="ACG8OL_000057"
     CDS             52229..52807
                     /locus_tag="ACG8OL_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4816080.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            53208..54119
                     /locus_tag="ACG8OL_000058"
     CDS             53208..54119
                     /locus_tag="ACG8OL_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4816081.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(54207..55124)
                     /gene="rnhC"
                     /locus_tag="ACG8OL_000059"
     CDS             complement(54207..55124)
                     /gene="rnhC"
                     /locus_tag="ACG8OL_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS4816082.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            55326..55763
                     /gene="zapA"
                     /locus_tag="ACG8OL_000060"
     CDS             55326..55763
                     /gene="zapA"
                     /locus_tag="ACG8OL_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS4816083.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            55822..56370
                     /locus_tag="ACG8OL_000061"
     CDS             55822..56370
                     /locus_tag="ACG8OL_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS4816084.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            56485..58845
                     /locus_tag="ACG8OL_000062"
     CDS             56485..58845
                     /locus_tag="ACG8OL_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS4816085.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            58932..59246
                     /gene="trxA"
                     /locus_tag="ACG8OL_000063"
     CDS             58932..59246
                     /gene="trxA"
                     /locus_tag="ACG8OL_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS4816086.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            59428..61206
                     /gene="uvrC"
                     /locus_tag="ACG8OL_000064"
     CDS             59428..61206
                     /gene="uvrC"
                     /locus_tag="ACG8OL_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS4816087.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            61351..62250
                     /locus_tag="ACG8OL_000065"
     CDS             61351..62250
                     /locus_tag="ACG8OL_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4816088.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            62243..63505
                     /locus_tag="ACG8OL_000066"
     CDS             62243..63505
                     /locus_tag="ACG8OL_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4816089.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            63666..65564
                     /locus_tag="ACG8OL_000067"
     CDS             63666..65564
                     /locus_tag="ACG8OL_000067"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS4816090.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(65770..66489)
                     /locus_tag="ACG8OL_000068"
     CDS             complement(65770..66489)
                     /locus_tag="ACG8OL_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS4816091.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    66679..66726
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            66826..67158
                     /locus_tag="ACG8OL_000069"
     CDS             66826..67158
                     /locus_tag="ACG8OL_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816092.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            67145..69148
                     /locus_tag="ACG8OL_000070"
     CDS             67145..69148
                     /locus_tag="ACG8OL_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS4816093.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            69167..69637
                     /locus_tag="ACG8OL_000071"
     CDS             69167..69637
                     /locus_tag="ACG8OL_000071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS4816094.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            69658..70242
                     /locus_tag="ACG8OL_000072"
     CDS             69658..70242
                     /locus_tag="ACG8OL_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFS4816095.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            70257..71243
                     /locus_tag="ACG8OL_000073"
     CDS             70257..71243
                     /locus_tag="ACG8OL_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS4816096.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            71233..71544
                     /locus_tag="ACG8OL_000074"
     CDS             71233..71544
                     /locus_tag="ACG8OL_000074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS4816097.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            71566..73347
                     /locus_tag="ACG8OL_000075"
     CDS             71566..73347
                     /locus_tag="ACG8OL_000075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS4816098.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            73340..74716
                     /locus_tag="ACG8OL_000076"
     CDS             73340..74716
                     /locus_tag="ACG8OL_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS4816099.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            74723..75358
                     /locus_tag="ACG8OL_000077"
     CDS             74723..75358
                     /locus_tag="ACG8OL_000077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS4816100.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            75388..76743
                     /locus_tag="ACG8OL_000078"
     CDS             75388..76743
                     /locus_tag="ACG8OL_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4816101.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(76935..78329)
                     /locus_tag="ACG8OL_000079"
     CDS             complement(76935..78329)
                     /locus_tag="ACG8OL_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4816102.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            78421..79392
                     /locus_tag="ACG8OL_000080"
     CDS             78421..79392
                     /locus_tag="ACG8OL_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4816103.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            79389..80171
                     /locus_tag="ACG8OL_000081"
     CDS             79389..80171
                     /locus_tag="ACG8OL_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS4816104.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(80340..80711)
                     /locus_tag="ACG8OL_000082"
     CDS             complement(80340..80711)
                     /locus_tag="ACG8OL_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4816105.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            80845..81288
                     /locus_tag="ACG8OL_000083"
     CDS             80845..81288
                     /locus_tag="ACG8OL_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4816106.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(81331..82140)
                     /gene="yidA"
                     /locus_tag="ACG8OL_000084"
     CDS             complement(81331..82140)
                     /gene="yidA"
                     /locus_tag="ACG8OL_000084"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS4816107.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(82159..83526)
                     /locus_tag="ACG8OL_000085"
     CDS             complement(82159..83526)
                     /locus_tag="ACG8OL_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4816108.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(83510..84340)
                     /locus_tag="ACG8OL_000086"
     CDS             complement(83510..84340)
                     /locus_tag="ACG8OL_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS4816109.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            84503..84940
                     /locus_tag="ACG8OL_000087"
     CDS             84503..84940
                     /locus_tag="ACG8OL_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS4816110.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            85016..85636
                     /gene="rpoE"
                     /locus_tag="ACG8OL_000088"
     CDS             85016..85636
                     /gene="rpoE"
                     /locus_tag="ACG8OL_000088"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS4816111.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            85825..86331
                     /locus_tag="ACG8OL_000089"
     CDS             85825..86331
                     /locus_tag="ACG8OL_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4816112.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            86348..87247
                     /locus_tag="ACG8OL_000090"
     CDS             86348..87247
                     /locus_tag="ACG8OL_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS4816113.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            87334..88218
                     /locus_tag="ACG8OL_000091"
     CDS             87334..88218
                     /locus_tag="ACG8OL_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4816114.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(88410..88481)
                     /locus_tag="ACG8OL_000092"
     tRNA            complement(88410..88481)
                     /locus_tag="ACG8OL_000092"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(88446..88448),aa:Arg,seq:ccg)
     gene            88541..89875
                     /gene="rpoN"
                     /locus_tag="ACG8OL_000093"
     CDS             88541..89875
                     /gene="rpoN"
                     /locus_tag="ACG8OL_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS4816115.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            90024..91115
                     /locus_tag="ACG8OL_000094"
     CDS             90024..91115
                     /locus_tag="ACG8OL_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS4816116.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            91156..92157
                     /gene="gap"
                     /locus_tag="ACG8OL_000095"
     CDS             91156..92157
                     /gene="gap"
                     /locus_tag="ACG8OL_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4816117.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            92267..93457
                     /gene="pgk"
                     /locus_tag="ACG8OL_000096"
     CDS             92267..93457
                     /gene="pgk"
                     /locus_tag="ACG8OL_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS4816118.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            93561..94316
                     /gene="tpiA"
                     /locus_tag="ACG8OL_000097"
     CDS             93561..94316
                     /gene="tpiA"
                     /locus_tag="ACG8OL_000097"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS4816119.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            94432..95730
                     /gene="eno"
                     /locus_tag="ACG8OL_000098"
     CDS             94432..95730
                     /gene="eno"
                     /locus_tag="ACG8OL_000098"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS4816120.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            96129..97268
                     /locus_tag="ACG8OL_000099"
     CDS             96129..97268
                     /locus_tag="ACG8OL_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS4816121.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        32317 a        16614 c        20183 g        28196 t
ORIGIN      
        1 caaaaatgaa aagccaaaac gacaagtaaa atattttttt atataaatat ttgtgttact
       61 aattccgtta atatacataa gtgaaagcgt tttatataag aacttgaaaa gaaagaaggg
      121 atagacatga aaaaaagata caaattggtg atagctttgc taaacattac cctattattc
      181 agcttaagtg catgtggaaa aactaacgaa gaagtaacag caaacatcgc agatccaatg
      241 ataaaaaaag ccactacatt agaagtatat gtcgcaaacg gcaaactgac aaactatgtt
      301 gaaaatgctg caaaaatcta taaccgcaaa actggagcaa atattgactt aaaaattgtg
      361 aatgtagctt cgggaacagc aacagtacaa atgatcaccc caaaattggt aacacacgaa
      421 gatatgccgg atattatctc catggcagac acttcagcag caggtgtgct ggaaaaattt
      481 caagatgctt tttatagtgc tgatgaatat ggattttatg acaaatatgg cgatcagttt
      541 tatgatcaaa aattgaatat tttgcgtcaa caaacgagta atgaaacgat tattccttgg
      601 gctagtgatt ttaccccagc actttgttat tatcaaccaa aaaaatttgc tgaaataggt
      661 gtagattttg aagaaatcag ttcttgggaa gaatatatcc aggtggcaaa agcattaaaa
      721 gaaaaaactg gtagttttgg aattgccttg ccagaatctg gggaccaaga attgtttatc
      781 aacatgctag cccagcagaa tcaatctttg cttgatgaag aaggaaatat ccgtctgaat
      841 actaaagaag cgaaacatgc tgcaaagtta atcaaagaaa tgatcgactc agatattgta
      901 catttctacg gtgcacaaga tgctgagaaa gcatttcaag aatctgcaat gtttgttgct
      961 ggcggctggt atgctacgaa tatgtctttg aattttcctg atgcatctga caaatggcgc
     1021 atggctccac tgattccttt ttcaaaagaa aatccaggaa aaggtgcagt atcaggagga
     1081 tcaagttttt atgtccctaa agatgcaaaa aatccacaag tggcacaaca atttcttaca
     1141 tttatgctga cagatgaaga atgcttagcc aacgcactgg agctgggcgt ggctacatct
     1201 aactcattgg cctatcaaac agaagcagct gagaaaaagt ttgactacta aggaggacaa
     1261 aaatactatg aactattgaa taaaatcaat gaaaatacat caaatactgt tttaccagcc
     1321 acttattcag atgctacgtc ttatttgact accgctgcat ataactattg gcaaacaggt
     1381 gattttgaaa attcatatct aaaagaagca gaaaattttg ctcataagta cggcgtaaaa
     1441 ttaactggtg attaaaaaat attaggagga cttaacgatg atgcaatctt cgttaccaag
     1501 caagacaata aagaacagca agaaaaaagc aacagatacg tctcataaaa aaagtgctta
     1561 tggattggtc acaccttttg ttattttctt tattgtgacg attttgattc ccatcattat
     1621 ctcgtttgct tttagtttta tgaaaattgg ctactcattt gaatttgttg gatttgacaa
     1681 ttatctagct atcttccgag atccattgtt tattaaatct tacacgaatg ttttgattat
     1741 catggcagga tcaattccaa taacgatgct attagctatt tactttgctg ttttattgaa
     1801 ttcagcacag ttaaaaggaa aaggattttt tagaactgtc tattatattc caaccattac
     1861 atcaacggtg gctgtagctt ccgtattcat gacttttttc aatccgactg ggttattcaa
     1921 tggatttttg aatgcgctag gaattaacgg tgtacagtgg ttaacaaatc ctttttggat
     1981 acgaatatca atgatcatca cgatgatttg gcttagtgta ggatataaca ctattttatt
     2041 tttagctggt cttcaaggga tttctaaaga gatatacgaa agtgctgaaa tagatggagc
     2101 atcgaaattt cggcaattct ttttattaac gatccccatg ctaaagccaa tcattttaat
     2161 ggccacagtt ttagccacta tcaatggctt gggtaatttc aatatcccaa atatattctt
     2221 tgggacttcc aatggaccag aaaattcagc tttggtcgta ggagtcaatt tatataaaac
     2281 aagttttgaa atggttgatt ttggaaaagc ctctgctatt gcctggacga tggtgtttgt
     2341 atctgttata ttatcggcta ttcaatttaa attcggaggt gataaaaatg actaaacaaa
     2401 agatacaaag cagaattttt acgtacgtca ttattgtgtt tggtgcctta atatttttga
     2461 tgccatattt atatatgatt gtagcttcaa cgcaaagtaa tcaagaaatt ttaagtggcg
     2521 gaatcaattt caagtttggg gatatgctat taaaaaactg gcaagccatc atggcgagat
     2581 ataattatcc cttgattatt tggaattcga ttgttattgc tgtaatcagt acagttttag
     2641 ctacagccag ttctacaatg gcgggttatg cattagcaaa gtatcgtttt atggggaata
     2701 aactgttgtt caatacagtt atgctatcta ggatggtgcc tttttttacc acattgattc
     2761 ctttatttta cattatgagt cgtttaggat tatcgaatac ttatgcaggt gtgatcatcc
     2821 cttcgattgc cagtacaact tcagtgttta tgatgcgaca atatagcagt caatttccta
     2881 cagcactgat ggaggctgcc agaatcgatg gtgccaatga gtgggtgatt tttacgaaaa
     2941 tcgctgtacc gattttatca cctacgatta ttactagtgg tttacttatc tttatgggaa
     3001 gttggaatca atatctattt ccattagtca tgctttcaga taaagataaa tatacggttc
     3061 ccttaattat caaaaatatg acggtttcta gtacaggtga gccaattaat tatggcgcaa
     3121 ttatgctaat tttagcaact tcagtgatcc caatggtctt gatttatgtg tgggcgcagt
     3181 ctaaatttaa ggagaatgac attggagctg caaataagta aaaaaaataa cgaatgttaa
     3241 ccatctagtt cgttctagga gcaactccac attcataaaa attaaaagaa tggcgggata
     3301 tccatgaatg aacagacgaa ggaatggtca agaaaaattt tgaatgtagc ttctgaaaag
     3361 atagatgcgg aattagacag aatggggaat aaaattccat atatttctta caacggaaaa
     3421 tatacagacg taataaagga cagaggaatc gactggtgga ccaatggttt ttggagtggt
     3481 attttatggc agctctttca caagacaaaa cgagaaaagt tcaaagaggc agcaatacaa
     3541 caagaacaat tattagaatc tgccctgtac catttttcag acgttcatca tgatgtaggt
     3601 tttttattct taccatcggc tattgcacat tatcaacagt ctcaaaacaa acatagtcga
     3661 caactagggt tacatgcagc tacggtactt gctggacgct ataatatttc agggaactat
     3721 ttagtagcat ggaatgacca taaacctggt tggatgatta ttgatagtat gatgaattta
     3781 ccattactgt attgggcttc gagggaaact ggtgatccga ggttctctaa aattgctcgg
     3841 cagcatgcag atacaacagc taagtatcta gtgaggaaag atggatcaac cgggcatatc
     3901 gcagaatttg atgcagaatc agggaaatat attcggttac ttggcgggca aggatacaat
     3961 gagaattctt gttggagcag aggaaatgca tgggctttat atggattcgc gttaagttat
     4021 cgttacacaa aaaatccgta ctatctagct atagcagaaa agatagcagt aaattttatt
     4081 cagaaaatcg aaaaaactgg ttatattcct ctcattgatt ttgctgtaaa agattcgaat
     4141 attgatacca gtgctggagt atgcgctgca tgcggtcttc tggaattagc aggatatgtt
     4201 tcctctgtac gatcaaaaat gtatcatgaa gctgctatca atatactcca aaagacagaa
     4261 aagcactatg caaattggaa tatggatgaa gatgggatca ttggaggcgg agcagaagca
     4321 taccatagag aactgactta taatgtgcca ctgatctatt ctgattattt ttttattgaa
     4381 ggcttacttc gattagaaca gtcggaatta gctatttggt agtaaaaaga ggagaaaata
     4441 caatgaaaca tccagtacca ctattaagga ccagacagga ttactttgac ttatttaaaa
     4501 agataaatca accactccag ccgttttatc gagcaagtgg cgcacaaatt gattatatac
     4561 atcaaggggt aggatatgga aataaaattg caggaatgga aggattctct cgggttttat
     4621 ggggagcggg gcccgcaatt gaccaattag atgaacgatg gttgaaatta attctatcag
     4681 gaataaaagc tggaacagat ccttcccatc ccgattattg gggagaaata catgaaagag
     4741 accagcgtat ggtagaaatg cccgcaatgt tattagcgtt atatcatcat gaacaattat
     4801 tatggaaaaa attagataca ggagaaaaaa gccagattat tcattggttc tcgcaaattt
     4861 ttgaatatga atgtgcagat ggtaattggc aattttttaa gataattgtc ggaacaatta
     4921 tccaaaaatt tggttatcca gttaaagaat cagcactgaa agaagcattt gataatatag
     4981 aaaattgtta tcttcaaaat ggctggtatc gagacagttc aagaggtcgc caagattatt
     5041 ataatccttt tgcatttcat tattatggtt tgatttatag cgtactagaa ccagaaaagc
     5101 ccattagcca gatatataag aaccgagcta ttgatttttc tagagactat attcattttt
     5161 ttgcggaaga tggtgcaaat gtgccttttg gtcgaagcat gatttatcgt tttgcagttt
     5221 caagcttttg gtcagctatg ttgtgggccg atcttttgcc ttttaaatta ggtgaaatca
     5281 aggggttagt caatcggaat ataagatggt ggatgcaaaa agaaatattt gatgaaaaag
     5341 gtatcctgaa tattggttat acctaccctc aattgacatt gactgaaccc tataatagta
     5401 cgttatctcc attatggctg aataagattt ttttattgtt ggcgctacct gaagaccatg
     5461 agtattggac ggctaaagaa atgcctatgc ctattcgtga aggaactaaa cttctttcag
     5521 aagccaattt gctcatacaa catgataatg gacatacatt ttttttgaat gcgggacaat
     5581 tagggccaaa tttccataca ttgaccaatg aaaaatattt aaaatttgct tattctagtc
     5641 aatttggttt ttcgataccg cgagcgaatc aattgaaagc agaaatggca atggattcaa
     5701 tgctagggat tcagcgagcg gacacatata tcacgaccag ttttaagaat gagagcatca
     5761 aaacctatgg acagttcatc gttcgaaata atgtacgaga cattgtatgt aagaaagagg
     5821 tacttgcctc tacatgggaa cctactttac aggcaaaaat cagaacttgg cttattcctt
     5881 ttgaaggctg gcagattcgc gtacacaaag tagaattaga tacagaatat gttctatatg
     5941 agacagggtt tgctattgaa tgttctcctg agaaagaagg aataatcata gaagaagaca
     6001 aggaaaacta tagggtgagt gaagctggct tttcaggtat tatctgttta agtgacgata
     6061 ctattaaacg aaaatccaca gctattatgg gtttgcccaa tacaaacttg atgacatggg
     6121 aaaacacttt tattcctgga ttagagggta catttggaaa aggaacccat tggctcggaa
     6181 caggagtggt tgctcatcaa gatcgggatt atagtttaga agtatggaac aatcgtccca
     6241 aaattgattt tgatgggaca accgggctaa ctatccaaaa taaaaacaat aaaaaaagac
     6301 taaaattatg ctaaaggctg gtggggaaat gcttgataaa gtgatgtttg aagagataat
     6361 acatccagaa aaatggataa atgaatgtta taaagaatta atggaaaaag aatggaataa
     6421 tgagatcgag cgttgtcgca gacacacgga gaggttaatc aaacgtatca gtaacagtca
     6481 gtatcaattc ccaagcgagt gtcctataaa aggacgttat ccattaagag acaatattgg
     6541 ttggacagaa ggattttgga caggaattaa ttggttgtta tttgagaaga cgggtgatcc
     6601 tttatttcaa caaacagctg aaaaagcaga tatagatttt atccacagga ttgactgtca
     6661 aattatcact aatcatcacg atcttggttt tttgtattct ttatcaaccg tagcagatta
     6721 taaaataacg ggaaatcgaa aaatgaagga agcttcaatt gatgctgcag aaacattact
     6781 tcagcgtttt tgggagaagg caggagttat acaagcttgg ggagatccta gtgatccttc
     6841 acaacaaggt agaatgatca tggattgttg catgaatctt ccattattgt attgggcgag
     6901 tgaagaaact ggaaatcaaa aatataaaaa agctgctcag gctcatattc aaaatgcagc
     6961 agattattta gtaagagaag atggttcaac ttatcataca ttttatatgg atgtcaaaga
     7021 tggaaaacca agatatggaa gtacgcatca aggttatagt gataattcct gttgggctag
     7081 aggccaagca tgggggattt acggttttgc attaagttat cgttatttga aagaacaaaa
     7141 atattttgat ttaagcaaaa tattagccaa ttattttatg aatcggttgc cagaagatac
     7201 catttgttat tgggatttga tctttgatga ttctagcaga gaagaaaaag atacatctag
     7261 tatggtgatc atggtttgtg gattattaga attaatagaa tcaggtctat tagacaaaac
     7321 agaaaagaac ttataccagt ctgctgtttg gaaaattatg cgacaattga aagaaagata
     7381 tgctacaacg gaagaagaag atggcttttt aacacagtct gtttataata agcccaaaaa
     7441 taatggggta aatacacctt gtatttgggg tgattactat tatttagaag ctttagtgag
     7501 aatgactagt aattggtctt cttattggtg aaaaaggtaa atatggaatt tttgtttgat
     7561 aaaataaaaa aagaaaaacc aaaacagcat gaatggtttc tgttttggtt ttttcgttaa
     7621 ttttcatatt cttctaattc caagcttagt tcttcccatt ccgtcaattt tgtttcttga
     7681 tcttgtctag cagcttctaa atcttggttc agctcattga gttttacgtg atcttccagg
     7741 ttttctggcg cgaccatttc ttgttccaac tgatcgatcc attcatcgat ccgcgccatt
     7801 tcttcttcaa cctgagtaat ttttcgttga aggctacgga taagcttttg ctgttctttg
     7861 ctttggtaat aatcagattt gttcgtggga gcaggagcag attctggctc atcttgtgcc
     7921 aacaattcgg cgatttcttc ttcttcctgc tttttctcta aataataatc atagtctcct
     7981 agatagactt tgctgccatt ttcagagagt tcgacgactt ttgtcgcgat ccgattgatg
     8041 aaatatcggt catgggaaac aaaaagcagt gtgccgtcat aatcgatcaa tgcattttct
     8101 aatacttctt tgttgtcaat atctaaatgg tttgttggtt catccaaaat caaaaaattt
     8161 tctttgttca tcgctaactt ggctaaagca acccgtgctt tttcaccgcc acttagcaga
     8221 gggattggtt tttctacatc ttcgccagaa aataagaaag cacctaaagc acttcggatc
     8281 tctttttctg gagtggtagg atgctcatcc cacaattctg ctaggatcgt cttgtttgta
     8341 tgcagatcag cttgaccttg atcgtaatag ccgatggtga catttgtccc tagcgtttct
     8401 tttccacgga taaaagggat ctggtgaatg attgatttca gcaacgtcga tttcccgata
     8461 ccattcggac ctactagagc aatcgcttct tgacgacgga tatctagatt gattggttca
     8521 gataaggtga cattatcata tccgatagcg gcatcttcga tttgcaagac tacatttcca
     8581 gaaggctttt ctatactgaa tagaaagttt gcttgttttt catccccttg aggacgatcc
     8641 agccggtcca ttttttctaa tgtctttctg cggctttgcg ctcgttttgt ggtagaggcg
     8701 cgaacaaggt tccgagcaac gaagtcttct aatttatgga tttctgtttg ctgcttttca
     8761 taagccttcc attcacttgc taactgttct gctttcaaat ctagatattt cgaatagtta
     8821 cctttgtaat ggcgcatttt tcttctgctt aattcataga cttcattgac tactttatct
     8881 aaaaaataac ggtcatggga aacaatcaat aatgcaccag tatagctttg cagataattc
     8941 tcaagccagg aaagtgtctc gatatctaaa tggttcgttg gttcatctag aatcaaaatg
     9001 tcagggtgtt gcaaaagcat gcgagctaaa gctaaacgtg tgcgctgtcc gcctgataaa
     9061 gtatcgattt tctgatcata aaatgaagaa tcgaatttaa acccatgcaa tacggaacga
     9121 atttcatttt cataaccata gccgtttcga tctgaaaaat cgtgttgcag ccggtcgtat
     9181 tcttttaaat aagaactata agcaggtgtg tctggcgtac cttcgctaat gaaaatctct
     9241 agctcgcgca tccgctcttc catttgtcta acttgttcaa aagctttcag catctcgttc
     9301 caaactgttt cattggaatc aagtcctgtg tcctgagcga ggtatccgag agtcgctgtt
     9361 ttatttttag cgatctttcc ttcatcaggt gcttcaattc cagcaatgat ctttaataaa
     9421 gtcgattttc cagcgccatt tctgccaact aaagcgacac gagcgcctgt actgatttct
     9481 aaatgtatgt tttcaaataa gacttctgca ccaaaatggc gagcaatctg attggcttgt
     9541 aataatatca tggaatcctc ttttctactt tgtgaattct cctcaaataa aaaactattt
     9601 ttcaattagt agtctaccat aaattccatt atactgcgag atttgcagaa atgataaata
     9661 aatgaataaa gaataaaatt tcacaatatc tgagcaatgg aattgatttt ttcctataaa
     9721 tactgctatg atggagaaat caaagatgta attagacaat acgtggaggt catttcgtga
     9781 aagatcatat tattccaaag gcgacagcta aaagactgcc tttgtattat cgctatttaa
     9841 ggattttaaa taatgctgga aagaaaaaag tgtcttcaac tgaattaagt gaagccgttc
     9901 aagtagatag tgcaacgatc cgtcgagatt tttcttattt tggcgaatta ggaaaacgtg
     9961 ggtatggtta tgatgtagaa gatcttatgc acttttttgc aaagacatta aacgacgatc
    10021 aattgacaaa tgttgcgctg atcggtgtag ggaacttggg aagtgcttta ctaaaataca
    10081 aattccatca aagtaatagc atacgtatca gttgtgcttt tgatgtgaat gaggaaatcg
    10141 ttgggcgcat cgtagacggt atccccgttt atccaatgga agatatgtca gaacaaatca
    10201 agatccaaca aatcgaagta gcaattttaa cgatacccgc tgaaagagca caagaagtag
    10261 tcaatcagtt aacggaagca ggaatcaaag gaatcttgaa ctttaccgct gctcgattga
    10321 atgctccgtc agatgtattg atccaaaatg ttgatttgac gaatgaattg caaaccttga
    10381 tctatttctt gcattctgac gcaaatgcgc attccgaaaa tacagcagaa taaaaataaa
    10441 aagcgattga acaaatgaag gcaaaacttt cacttgttca atcgcttttt tctgtttgga
    10501 caacgattat tttgttcgga agatttcttc ataagaaagg gaatcatctt ctgctgtcag
    10561 ttcatctagt agataacgga tttttttaaa gtcgatgaac cgtgcttgcc gaaagacttc
    10621 ttttccttga tgaaaaatca ggacaactgg cgcagtcagc acttcaaatt tactagctac
    10681 ttctggtgtt tcaaatgcat ccaaatgaag tgccggaata tcataatgtg tgagaagttc
    10741 ctctagtcgt ggacgaacag cgtgacaaac actgcaatta ggcatcgaaa gataaatgac
    10801 ggcaacggat gactgttgca gtatgtcttg aacttcagtc aagttagtgg tttgtttcat
    10861 ttttattctc ccttttatga tagaagtgat atgtatgaga ccttaattta atgggaataa
    10921 cagaaaaagt caattgaagt gctttggtaa aaaataagag ttgtgtaaaa atttacagag
    10981 caacttgttt tttttgaata aaagcgtata tttgcaaagc gaatgaacta gaaataaaaa
    11041 ggaggaaact catgttttct tttattccaa attcggtttt gcagatgagt acgattttct
    11101 tatcgatcgt tattgaagca ttacctttcg tattattagg ctgtcttatc tcaggcgcct
    11161 tacaagtatt tttgacccca gaacgtgtga agagatggct tcctgaaaat cgtatcttct
    11221 ctatttttac tggttcgatc ttaggatttt tctttccttc atgtgaatgc ggcatcgtac
    11281 cgatcgttca tcaatttgtt aaaaaaggcg taccagttca cacagcgttt gcttttatgc
    11341 tgactgctcc gatcatcaat ccaatcgtga ttttttccac ttttatcgca tttggcaatt
    11401 cttggcagat ggcaagctgg cgggtgatag gcagcttttt tgttgccctt gtgattggtg
    11461 tctggcttgc ctattttcaa aaagacgctg tattgaaaga aaaaatcacg tctaaagtag
    11521 cagaaaatca ccatcatcat gtccatgaag aagaacaaag tgagaagaat gaaccaaaaa
    11581 atcagctgat ccatcaaact gggcatgtgt tgacacattc aatcgacgaa ttttttgata
    11641 caggtcgtta tttgatcatt ggcggattaa tcgcagctgc gatgcagact tatttaccaa
    11701 ctagaattat gctaacgctc ggctcaacaa agattttagc cattatcatc atgctgttat
    11761 tggcctttac gatgtctctt tgctcagaag cagatgcgtt tataggatct tctttgttaa
    11821 gtctgttcgg cacagcacct gtagctgcct ttttggtctt tggcccaatg gtcgatatca
    11881 aaaatctact gatgatgaaa cgatatttta caggacgctt tattttatcg ctgatcggac
    11941 tgattgccct tagcgttatt ggattcacat tatttattta ggagggaaaa tatgctgcgg
    12001 tttttgattt tatctgggta tttcgaattg atgatgtact tgcaagtttc tggcaaactc
    12061 gatcaatata tcaatgtcca ttatcgctac ttagctattc tttcaatgat tttatctgct
    12121 cttttggctt tagttcagct gtatttgtgg gtcaaaaacg gaaaagcaga agaaaaggca
    12181 cacgagcacg atcatgacca tgggttgtca aaaccttatc aacgtggaat cgcttatgta
    12241 ctgctagctt tgccagtaat cgtggggacg ttgtttccaa cagttagtct cgacactacg
    12301 atcgtagaag ccaaaggatt taatttccca gtaagtaaag aatcagtagg ggatccggag
    12361 atgcagacac aatacttaaa gccggatacc agtatttatt tcaacaaatc agactactta
    12421 gaccaaatga atgagttgaa agaaaagtat ggggataagc aaactatcaa gatcacagat
    12481 gaaaactatt tagaagttat ggagctaatt tataattacc ctagcgaatt cattggcaaa
    12541 aaaatcagct atgaaggatt tgtttaccaa acaccgaacg atagcaatac agatatcttc
    12601 ttgttccgat tcggcattat ccattgtgtg gcagattcag gagtctttgg tttgttgacg
    12661 catatgccgg aaggagtaac ggttaaaaat gatgaatggt ataaaataga aggaaccatc
    12721 caatcagact attatcaacc attcaagcgt gaaatcccat ctgtagttgt tacaaacgct
    12781 gagaaagtct ctgctccgaa aaatcagtat gtgtatcgtt ctttttaaaa gaacaaaata
    12841 aataaacata gctcagacag ctgacacgca aggacgtcgg ttgtctggct ttttttgcat
    12901 gatgtattca tttgtttgat atttatttta gaagcagcat cctgaaagta tttgatatcg
    12961 gtttcaaagg tgttggaaaa gattgtgaag agaattacaa tttattccta acatgtatgt
    13021 tgaaaagttg tatgaaatga ttttgatagt aaaatatcga gtggaggtac tttttatggt
    13081 aacgcgtata tttattttgg atgatagttt ggtttttgaa aaaatgctag tagaaatttt
    13141 ggaagaatcc agaattttat taagtccttg gaaatttgag atcgttcagg gattgaacgt
    13201 tatacaattt gttgaattca ttaaaaataa tgaaatctat tcctcagata tttttttcct
    13261 agacatcgat ttgaacgcct attatagcgg tatcgaacta ggcgaaatgc ttagagaaaa
    13321 gaatgaaaag tgtaaaattg tttatctaac gtctcatccg aataaagcaa taactgttat
    13381 taatcgaaag attacgccta gtggttattt gataaaagaa ttcacttatg aaaaaaataa
    13441 aaaaagcatc caaagttact tattgaaaga aaaagaaaac tatcctgttc aacatgctac
    13501 gaaaaaaagg tggacaactg tccgttatgg taatagcgat cactatatcc attacgatga
    13561 aatcctttat ctaatgagca tgccaggtta taaaaataaa ataggcctag tattgaaaga
    13621 tgaagagatt ctcgtaaacg gcacaattaa aaaatataaa aaatcactcg aatatgagta
    13681 tttatgtaaa gatttaaaag cttatatcat taatactgaa aacattacat ctattagcag
    13741 agtaaacgaa atagtaacat ttgcaaatgg agaacaacta gagatgggaa agagaagtat
    13801 tgataaacta aagaatttta tgcaagaaaa gatagaaaat gaatcatttt aaagaaaaca
    13861 aagactcata tcttacctgt tagaagagtg ttttcctgta gaagagaaca acccaagtag
    13921 cagcctagtc gtttctatcc ttttttgtta tgcactcttt ttgacattta ctccaccaat
    13981 aacgaagggt acaataaaaa gtaataacaa acaaaaggag tacagaaatg attgaattca
    14041 tcaatgtaag taaaagttac gggaaaaaac aagcattaaa agacttgtct ctctcgataa
    14101 gacaagggga aatttttgga tttttaggac ataatggtgc cggaaaatca actacgatta
    14161 aaagtttagt tagtattatt gaaccatcat cgggaacgat tttagtagat ggaatgaaat
    14221 tgacggaaaa tcgattgagt atcaaacaaa aaatcggtta tgtgcctgat tccccagata
    14281 tttttttaca attgacggct ggagaatatt gggacttgat ttcagctgcc tatgaattag
    14341 acacacaaaa aaaagaaaag cgtttagcag aattgacagc gctttttgat atgtatagtc
    14401 atcaaaatga aacaatcgca agtttttcac atggaatgcg tcaaaaaacg attttgatcg
    14461 gcacgttgct gccagatcca gatatttggg tattggatga acctttgcaa ggacttgatc
    14521 ctcaagcagc atttgatttg aaagaaatga tgaaggctca tgccgcaaaa ggaaaaacag
    14581 tcattttttc tacacatgta ttggataccg cacaacagtt atgcgataaa ctggctattt
    14641 tgaagaaagg tgaattgatt tatcaggggg cagttagtga attattgaat ggctcacctg
    14701 atgaaacgct agaaaagatt tatttgaaaa tggctggaag gcaagcgaca ggagatggac
    14761 cgtatgtata agcgacaatt attagaatta attcgagtta atcttttata tgttaatcca
    14821 cagctcacga ataaagcaag agagaaagga aaaaagggaa agcgattaac tatctatctt
    14881 ttgtctcaaa atcttttttc agggctgatt tttttactga tttacggatt tgctatgttt
    14941 gctatgaact ttgttaaatt gccaggtttc tttacttatt acgtggcact ttttggtatg
    15001 ttaggtttaa ctcaaagtat atcagtcatt tataatatct tttttgaagg aaatgacctg
    15061 caagtatttt tgcctctgcc ttttggacaa gaacaggttt tttcagcaaa gattttggta
    15121 gttgctttga ccatcattcc ttttgttttt ccgcttctag tcatgtttat tttaaccgga
    15181 tggcgagctg atctagggat tttgcttact atcattatct caatcctttt gttttctcta
    15241 tttttgatta ttcttttttg tctggctagt ttgattgttt ttggtttggc tcgaacagca
    15301 gtgtttaaaa aacatagaaa aacagtttct agtatcttgc ttggcagctc gatgctgatc
    15361 gcatttgctg gaatcatgtg gatgaatcat caaacgggga ctattgaaaa tgagttaaca
    15421 gatcggcatc cgatttcgat attattgcca ttctatgatg ctgccagtca gccactttct
    15481 caacaaggtt tattgggttg gggaatacta gctatcacgg ctctgggatt tttccttgct
    15541 gtaaaacagc tgatagtacc aaagcttaca gagcaactga ctgattcaaa cagtgagttc
    15601 aaaataaaaa gaacccataa aaagaaccaa aatgtgcatc agcttttatt tagttacaat
    15661 gctcaattag taaaagaacc taatttgatt atgcaagttc tttcttcttc cttgttaact
    15721 ccaatcattt ttattcttgc ttttgcccta ggaggcgaaa taaaattaac tgatttagat
    15781 aataagttaa tcggtgttgt tttcctggta gggatcgcat tggctttctt aacagttaat
    15841 cagacttctt ttatcagcaa tcttatttct ttggatcaag aaaattttct gtttatccgt
    15901 tctttgccaa tctctatgaa ccaatattta aaagaaaagt tcagattcgg atggatattg
    15961 cagagttttt taactggtgg gatcgcatta ttaagcggac tgtcttttca gctgcctttg
    16021 ttttttatcc ctagcttgct atttggttct ttattcggct gctacttatt gtcactacgt
    16081 tatttcgcta gagattatcg gttgctgctg ctgaactgga ccaatattaa ccaattattt
    16141 aacagaggtt caggaagttt gggtttagtc gcaactatga tgggttcatt gatcatcagt
    16201 attattctta tcgtcttata cggaatgttg gctttgtttt atccattttg gttagtgaat
    16261 attcctgtga tcggtttagt tctaatactt tccgctctat ggatcgttta ttatcaacga
    16321 acgttttgga aaaaatttga ataaagaaga ggggccgtga caaaaatcat gtcgcagccc
    16381 cttttttaca gatagagggt gttcaaaagt cagtttctca gtattaagtc aattttatgt
    16441 aaaaatgtga gaagctattt ttacaaaact ttttttaata ctcataatta cttcttattg
    16501 ctcctgcggt tacttgtcgc aatgtttgtg agatattttg tcatcgctca aggctgaacg
    16561 tctctttcac aaccttttct ttgttacact tcgtttattt aaaaagccgt ttttttcgtt
    16621 tcatcatgat tgcgtcacgc tttactttga aattacggat gttatggtta aagacttctg
    16681 ataatacaag acctaaagcg attgctccag caatcataat tgcttggacc gcataatgag
    16741 ccgcttctcg ataggattca gtaacgagat ttcgaaccgc ttgataagct aaaccaccag
    16801 ggactagagg aaccatcccg ggaatattga aaatcgttac gggcattttc aaacgtttag
    16861 agaaaagatc gctgatgaat gctactccta aagaaccaag caatgaacca aaagcagcag
    16921 atccgcctat ttgaacacag atccagtaga tcatccagcc aaaagcacca gaaagaccac
    16981 aacaaatcaa tgaacgccta ggtacatttg taataacggc aaatgccgca gtcgctaaaa
    17041 aactaaaaga aaactgaata aatatcgtcc ccatacttgt gttcctttcc taataaaaaa
    17101 gctggaaaat aaaagcaatc gcaaaaccga tcatcgtagc agtcatcatc gcttctgttc
    17161 cgcgagaaac tcctgagaca taatgtccag ctagcaaatc acggatagcg ttcgtaattt
    17221 gaactccagg aactaaaggc atcacacatc cgataatgat tagatcctga ttgcttccta
    17281 agccaaaacg tacgctgtag atagcggcta tgccaatcaa taaagaagat aaaaattccg
    17341 ataaaaattt gattctaaaa aatttcaaac tatatagata agctgcgtat cccaatcctc
    17401 caatgactaa agtcacaggt aaatcggtca tctttccgcc aaataacacc ataattgttc
    17461 cactgatcac tgccgcgcta acgatttgca accagacagg aaagaaagaa cggtctcggt
    17521 cgattgcttg cagttcgcag taaagttctt ctaatgttag ctctgaagca acatatttac
    17581 gtgaaagatt atttactttt actacttttt ctaaattaat cgaacggttg gtaatttgtt
    17641 ccattcggat ggtcgaggta cgatcaagac caataaagag accggtttga gtgacatagc
    17701 tgaccaaacg gaaatttcca gaagctaaag caattcgact catcgtatct tctacccgat
    17761 acatctccgc attgctttcc atcatgattt tcccagcgag taaacaagtg tccagcaaca
    17821 gatctaattc ttttcccgtc atagcatctt ctccaataat tgattgatta gttttatttt
    17881 actacaaaac ataccagaag cgtaaagaat aaaaaaactt gaggaaggaa taaaagaggc
    17941 gtatactgtt tttgtattgc gggtgtagtt tagtggtaaa atcccagctt cccaagctga
    18001 tgtcgcgggt tcgattcccg tcacccgctt aaaataggtt gtgaaaaagc tgctctgcaa
    18061 caagtaataa gaacagccaa gcaaaaacaa cttcttatgt ttttaacttg gatggactta
    18121 ttaccgcaga tgcaagcttt tgcgcaccgt ttaccaagcg gttgtgaaat cagtgtattg
    18181 tcttgagtat gaagaaaatt tttgaataat aataaatacc ttacagatag agtggtgact
    18241 gattagagac gaggaatccg tctctttttt gtatttttaa gccatcttaa aacaggtttt
    18301 attttttgct gtttctattt ctttctagta tttatttata ggggaacata tgttaagatt
    18361 tagggagctg ttagtaaagg agtaaagaaa tgagcaaaaa aacaaccaag gcgatcgtgg
    18421 ggatcgtcgc tgctgtgatt gcaggggcat tcggtgtttc caccacacaa aaagattcag
    18481 atatctttca gcagattaca actatttttg attccacacc acaagcttct caagtagcgg
    18541 atgtgccaga atatgatgga aaacatcaag aaatagaaat caatgggaat cagccggaat
    18601 ttacaaaaga ggatcttagc ctttccaaag gaacatggga aagctattca aatattgata
    18661 aattgaacag agtaggacaa gccaatgcta tgcttggaaa agatttgttc cctactgaaa
    18721 agagagagcc tttgtatatt gacccgacag gttggaaaca aaagaaatta agtgacgggc
    18781 aatggttata taatcgcagc catttaattg gtttccagct gactggtcaa aataacaaca
    18841 ttaaaaactt gatgacaggg acacgttcgt tgaatgctcc atacatgctt gctcacgaaa
    18901 atgatattgc ggcatatatc aaagaaacaa atcatcatgt acgctaccgt gtcactcctc
    18961 attttgaggg gaatgaactg gtagccaggg gagtgcaatt ggaagcagaa agcattgaag
    19021 acaaaaaaat tgaattcaat gtctttatct acaatgttca agatggctac acgatcaatt
    19081 atgagacagg acaagcaaca aaaacaaaaa attagtagca gctattcgaa ttttttatga
    19141 atattatgaa aaaattgtgt aaaaacatac attcaaagaa atatagcgta tactttatga
    19201 aaagagtact tttgataaag ctacagaaaa aaggaaaaaa acatacaaat agtgtttctt
    19261 aatttattcg gaagtttgga atatttgttt gtagttttcc ctttcttcga gtaaaataag
    19321 gttgtagaag gtaagctgga attaccttgc gggaaagagg tgtagaatca tgggttttac
    19381 agatgaaacc gtacgttttg gttttgacga tagtcgcaaa aaagaagtga gcgaaacatt
    19441 agcgattgtt tatcgggctt tggaagaaaa aggctacaat ccaatcaatc agattgtcgg
    19501 ctacttgctt tctggagacc cgtcctacat tcctcgttat cgtgatgctc gaaatttgat
    19561 tcgccgtcac gaacgcgatg agatcatgga agtaattgtc aaggactacc tctcaaatca
    19621 tggagtgaat ttatgagagt aatgggcttg gatgtcggtt ctaagacagt aggtgttgct
    19681 atcagtgacc cctttggctg gacagctcaa ggagtagaga tcattcggat caatgaagca
    19741 gaagaagagt ttggctttga tcggttgaag gaattagtag cacaatatga agtagaccgg
    19801 tttgtagttg gattacctaa aaatatgaac aattccatcg gaccaagggc agaagcttcc
    19861 atgtcatacg gagaaaagat caaagaacta ttctccttgc cagttgatta tcaagatgaa
    19921 cgattaacca ctgttcaggc agaacgtatg ctagtagagc aggcaaatac ttcaagagcc
    19981 aaacgaaaaa aagtcatcga taaattagca gctgtgatga tcttacaaaa ttatttagac
    20041 ggctcaggta agcaattatt ttaataataa aagaaaagag gtaatcttat gacagaacat
    20101 aaccattccc acgaccatga aggacacgaa cacatcacat tggtggatga ccaaggaaat
    20161 gaaacattat atgaaatcct actaacagtt gacggacaag aagaatttgg acgtaactat
    20221 gttcttttat atcctgctgg tgtttcagaa gatgaagatg tagaattaca agcttatgca
    20281 tacatcgaaa acgaagatgg aacagaaggc gagttagaac aaatcgaaac agacgcagaa
    20341 tgggacatga ttgaagaagt attcaataca ttcatggcgg aagaagaaga atagactata
    20401 aagctaggag aaaccttgat atgatagggt ttctcctttt tttatttttt actgtatgcc
    20461 atattgtatg ccatctagtc aatatttaag tgttgtacta atttttcaac agtcttttct
    20521 ttcgtttctt ttgccaagtg accgtaacaa tcgatcgtct gactaatttt tgtatgccgt
    20581 aatctagttt gaacttctac catagtagct ccagcgtcca agagcattga agcatgagtg
    20641 tgtcttgcta catgcacacc gagaaatgga accatagctt tttcacaaat agtaatcaat
    20701 cttgcataaa tagcgtggtg tgtgattagt tctttataga taccacaaaa aatcatagtc
    20761 ggatcagtta caccaagtgt cagcatatat ttccgctgat taatctgcca tttttttaat
    20821 atagaaattg ttctttcatc tagtggaaga accccagcac tacaatcagt tttaggatca
    20881 ctgattttat agccattttt ggattggctt agtgttttat tgatggtgac cgtattttct
    20941 tgaaaatcaa tgtctgacca attcaatgca agggcttcac tgatcctaac cccgctataa
    21001 aagagaaagc gtagtaacgt tttatcaaag tcgtttcgat aagtttcagg tttgctgttt
    21061 aggtaatcaa acaaggtgat cagttgttct ttactgtaaa ctttgacctt ttctttatgc
    21121 ttagtggtgg ttcttttagg cattaatacg ttgtctagtg gattagtctc aataatccca
    21181 agattgatag cgtagctaaa aatacgctta gaagttgaat gaagtacctt gtattggtca
    21241 gtagtttcag cccagctatt tacaatttgt tgggcttttt ttaccgtgac ttcattcaca
    21301 cgaagatttc catattttgg aagaatccac tttctaaact tgatttcagt tttcatgaag
    21361 gtggcttctc ggacagtggt tttatatgtt tctagccaca agtcataaac ttctttaaac
    21421 gtttcgttgt tcgctttttt aataccgttt ttatcgtagt ccacttgcaa gcgtgacaag
    21481 gcaagctggg cttctttttt tgttttgtaa cctcgtctac gaaccttatc aggtttgcca
    21541 gtcattttgt tcattccaag ataggcttca tagagccaaa atttaccttt tttagtttca
    21601 tattgcttga atgttgccat aatagttttc cctccatagt gtagcaagcc gtgggggcgg
    21661 ttggaggagg cagtttatta ttattttgaa tagtgtttat ttagctcagt aacaagagct
    21721 gtgaactctt ttagaagatc attttcataa aattcttgat actctttttt ctcatctgta
    21781 tttttttctg aatcattaat taatgagtca tatcgtaaaa gcgtatttag aagagctgcg
    21841 agataagcaa ttggggtact cccgtcagga aattcaattc gagatcctgt tttaagatat
    21901 tttattgcgt ttgttaaata aatcttctgt gagacagtca ggttatcttc ttcgatgtta
    21961 ttgatagcat tagaaagatt agaatcaatt atagagttat ttttttcaat agtttttttc
    22021 aattgctttt caagcacatt tcgcttaatt tcgggcatca tatgcatatc tcttaatgta
    22081 taagatccgt ttaatagata ggtagtggat acatctaata cctttgaaag ttgtttaagc
    22141 ctggtgttat tgggaagatt atagccattt tcccaattac taacggcacc tttactggcc
    22201 ggtggggata atttacctcc aagttgttcc attgagagtc ccttttcaat tctagccagt
    22261 ctaattcttt gtcctgtctc cactgcttcg gggtattttt ttctagccac attttcacca
    22321 ccattcatat attttctcta ctatatcacg atttaaatat gtgtgaaaca taaaagttca
    22381 aaaaagtatg ttaaaattct tgcaaatgaa aaggtatgat ggtatattga gtttataaga
    22441 ggtatagaaa tgtattcaag agcctcttgc atttttttaa aatctttgta tagaaatgta
    22501 tgctaacgag gggggataaa tatggaatta aaaattgaca aactcacgct tgatgatcca
    22561 gcttttaaaa ttattgacga tcgagttgtt caacaagtag agcaaactca aggaacgtta
    22621 ctttcattat tagaaaaaaa gtaccagttt ccaaaatata tgaacaaggc acaagctgct
    22681 aagtatatga acgtttcata caatactatg atgcagaaat atgttcctaa cggtttaaag
    22741 ttgattatag ttgatggagt cgtaagaatt tcacaagatg aatgtgatcg ctttatggaa
    22801 gaataccaaa aataaacgca agccgtgggg gcaaagggat taataggagg aaataataat
    22861 ggaatcgaca caaaataaag caatcgaaaa agtattatca acaattctta cagaagacac
    22921 agcggcagaa ttagcaaact tggaaggcaa agcgctagaa gaaacgtttg aatgtttgta
    22981 cgaacaaatg gactatcaaa aattgctacc acaagaacca acggtaagcg gagtattgcg
    23041 tggcttgaac gatttagtac aagcagagtt taaagaacga ctttctattg aagagtatca
    23101 agaaattttg tatcagcaag ttgagttgtt agcgagcttg ctagggcttg aactggagga
    23161 cgttgaatga aaactgaaat ttggaacgga cacattataa gatttgtcga tattaatgat
    23221 gaatggtggg cagtggcgaa agatgttgcg gaagcgttag gtcttaaaca ggttactaga
    23281 gccattcact ctttgcctaa agatggggtt actactagta aggtcatcga ttcattgggc
    23341 agaacacaag atgtaaacat catcaacgaa aaaaaatatt taccgcatgg cattcaaaag
    23401 ccgtaaaaaa gaagccgagg catttcaaga ctggattttc gacatcatca aagaactacg
    23461 ccagtccaca ggacttgaag gctttcaagt attccgcatg ctagacaaag aacaccaaaa
    23521 agaagcaatg actaaattaa gccacgctat tactgaacca aaaccagtag actacattaa
    23581 agcgaatgtg atagccaata aagctgtatc aactatttat ggtcattcca aaatggtaaa
    23641 aaagaaagat atgactcctg aaatgttggt tgatcgtgaa cctattttag atgagacagt
    23701 agaattaatg actgttaaag aaaaatatgg gttgcagttt tcagtgagtg agaaaattta
    23761 taatcgttct gctgaattgc agacaactta ggagcattaa catgatcgag aacaaagaaa
    23821 aagccttatc cataacagtc ttggcggaca ggataaaggc aacttaattg ggataaaacc
    23881 cgatattctt tgttccgatt ataccatatt aaaaaatcgg agggaaccat tatgaaacga
    23941 aacttaatta taggaagcat tttaatcggc ggaattttcg gcttattacc attaattgca
    24001 aaaatagcta tctttgcagg attagcgtta tatctcttca ctgcttatga cgagtacgac
    24061 tatcaattaa gaactggagg gcataagtag atgtacaaaa caaaactatt aaaccaactc
    24121 gatagccttg aattagagga aattaatcaa gggatcgctg aactagaaaa taatatcggg
    24181 aaaacctact ttggaaattc attcaatgaa aaactaacag ttttgtatgt actcaaaaaa
    24241 catgcagaac acaaaataat ttgtcgagaa atcaacgagc taaaaaatca gattttaacc
    24301 gcatggttaa acattactga tatgcaagaa gcaagggtga aaacatttaa tacatgggta
    24361 aagtatcaga atcaattaaa aggtgctgaa tttgttcggg atgggttgaa atacgaactt
    24421 gagcagttga agctcatgga ggtgtctgaa tgatttatac cagtattttg aaaaaggagg
    24481 agaggtttat tggcaaataa ttatcaagca gcaacggaat tacaaaagtc gggatggtca
    24541 atttatccct tatctcctgg aactaaaatt ccaataaaag gctctagtgg ttggaatgat
    24601 gcaactaacg attctaatca aattaatgaa tggtggacgg aagatgcaac tagaaatatt
    24661 gggttaatgc ttgaagatca tcaaatggtg gtagttgacc tagatcaaca ttctgaaaca
    24721 cgaaacggcg ttgagaatta taaaaaactt gcttcaatgt atgatccatt tccttctact
    24781 tatacggaaa tcacaccagg taaaggaatc catttcttct ttaaaattcc tgatggtgta
    24841 attatccaac aagaaacagg tgcatttgct gatatcttcg ggcgtgatga taaaggtaaa
    24901 agtttaagcg ggattgatat tatcactaaa ggagttccaa tagcgccaac tatgattatt
    24961 tcaagaagtg ttgggagagc atatgaacct attgaaaacg tatctctaaa taatattgca
    25021 ccagctcccg attggctagt gaacctactg ctaaaaaaac cgcaagaaat aaagactcaa
    25081 taccgacccg tagcaaaaac agggacagca aaaaaattag atatgattgt tcaaggcgct
    25141 ggcgaaggta gtagaaacga tcatttaact aagttatgcg gttggttact atggcacggt
    25201 gtagataatc aaactttagc agaattgatt tatacagcaa atgcgtacaa tgaaccaccg
    25261 ctaaaagata aagaagtgaa tcaaattatt cgatcgatga ttaaaaaaga tttgaggggg
    25321 aaagctcatg gctcataagc ataaattaga tagtatttta gactttcccg aagccagcga
    25381 gcgtgaagac aatatcattg aattgaaaac ctggatgtca cgattaagat gcaataaaga
    25441 cgatcagatt aaaagtaaca gtgttgtgaa tgcagaattg attttaacca atgatagcaa
    25501 tttagctgga acaattgctt ataacgaatt tagcggatac attcacctat taaaagattc
    25561 tccttggatc aatcgtaccg ctggagaatg ggaagacagt tttgaggatg ccttaacggc
    25621 atatattgaa gaaaactaca atgtagtttt tgatgacaac aaaatacaca aagcagtagt
    25681 aaatgtggct agaaagaatg ttttcaaccc agtaaaagaa cgtatcgaaa aagttaaatg
    25741 ggatcaacag cctagactgg aaacactgtt tattgacctt ttaggggtag acgacaacct
    25801 ttacacacga gaagtaacaa aacgttggat agtcggatca gtcgcacgta tctataaacc
    25861 aggaataaag tttgagatcg ttccagtttt ggatggaccg caagggattg gtaagagtac
    25921 cgttccagca ttactttaca cggatgattt tttcaccgat tcattagatt cgctaggtga
    25981 aaagaaagat gattatatgc agcttcaagg caatgtaatt attgaacttg gagaattatc
    26041 ttcaatgaac aaaactaaaa ttgaacagat aaaaaacttc atcagtgcca agatcgataa
    26101 aattagacct ccttatggtc ggaatgtcat agcttgggca aggcaatgtg tatttattgg
    26161 aacgtcgaat gatgggcagt atttgaaaga tgatacagga aaccgccgtt tttatccttt
    26221 gccttgtaag aataagccaa agatgaatcc gtttaaaaca aaggatgaat attttcttca
    26281 agtgttagct gaagcaaagg tgctttttga taaaggacaa cgaatctatt ttagtcccga
    26341 tgaagataaa gaagtcttag aaattgcaaa agactatcaa gaggatgcca agatggagaa
    26401 tccaattaaa gaagcaatca ctaaatattt ggaaatggaa atcccgtata aatgggaaga
    26461 agctccagcg tgggcgagac gttcttatta tcagaactat cctgacagta aatgtaatga
    26521 gaaaaattta cagcatttct ataatggagc tactatgcaa caagagtttt atttagttga
    26581 tagcgtactc accgctgata ttttggaagc tgtttttgat aaacaagcaa aggatttatt
    26641 gaatggtcga tcagatgcag aaactaaaaa gattgccttg attattagca gtattcctgg
    26701 ttgggaacgg aagcaactgc caaaaagaaa taaacgacga ggattttaca ataagtcaaa
    26761 tgcctatgaa aataaaaatc gagctggaaa gtacaaaaag taacgggtag taacgggtca
    26821 gtaacaccta gcccgttact agtttaccct tactgcccca agggatacag caatttggta
    26881 acggggtaac accttatttc cttaaaactt ttgaaatagt tagctaatat gctggggaat
    26941 aatgttactg gattttaccc gttacctgtt accaaaacgc tagaaccctt gtccccgttg
    27001 ggattgagtg gtaacgggta cctgttacgt aggtgttacc acgtgttacg aatcaaggag
    27061 gaaagtaaat gctcaatatt atttcaacaa acaaagcacc gaactttcaa tatacagatg
    27121 aaatggatcg gtttctaatg aacacgctgg catttagtgt gggattggta acagaagatt
    27181 attccacatt tgatccagaa gtattaaaga taatggaaga agaacctgat tggctgcagg
    27241 agtcagtagc gtggtgtcaa tcgctagtag tgggatcatt ggtagatagt ggcaactatg
    27301 atgatacagg tgagctaatg gatgaattta attgtctgct taacctatat gatcgagcga
    27361 gacaacgaga gcttacatcg aatgaggata atttgttttt aaacatccat gataagttct
    27421 tggctttgct actaacagat gatgaattga taactaattt attggaggta gaataattat
    27481 gcatatggaa agtattagat acttcgatgg tgagaaacac attgagtatt caaacactca
    27541 agaagatgta gactttatta gttttgagga agataaaatc actaaagtta attttaaaga
    27601 cggaacgtgc ttgaagatag tttcgcctta tattgaatac aaatcaaaat gggaacaaga
    27661 tgattctgac gaacgagatc gctttggttg gtaatctatg gcagttaaaa aacaatgtaa
    27721 tcatgctgga tgtaaaacat tgattgatta taggcagaag tattgtggga agcataaagc
    27781 aaagcaaacg gcaattaagc gtgaagaaag aaagcaaagc gaaggtaaat attttcaatt
    27841 ctatcaaagt agaacatggc gaaaggcttc atacttgtat cggttaaatc atccagtatg
    27901 tgaggattgc ctagaagaag gcttgataag gaaagctgat gttgtagatc acaaaattga
    27961 actaaaagat gattggaata gacggttaga tgaaactaat tttcgttctt tatgccatgc
    28021 acaccataat tccaagacag ccaatgaaag acaaagacga gaaaagagta ccctttgagt
    28081 taagggggca tagtgtgaaa cttactgaca atcgatgcct actcattttg gtacaaataa
    28141 ccgttgtgaa aaggcataag ggtaattaca aataacgatt acacttgtaa tcacaagtga
    28201 aaaagtatat aatgaaagta ggagatttat tgaaaaatac aattgttatt actgataaaa
    28261 cagggaaaca gcgcaccatt gagttacctc attttggtca agtgaccatt caaatgcaga
    28321 acggaaaaat catttatata gataaattag ataaagaaaa attctgatcg aaaaacgaag
    28381 gaatgtgagc ttaattgctt acgttccttt ttcttttgtc tgaaaggagg acaacatggg
    28441 acaccctaaa atattacacg atacaaaagg caatatatca agtgaagaaa aagcaatccg
    28501 tgtggacgct agagaagaat tgttcaagca gcaaccatta ataaatatca cgccccctga
    28561 ctggatggca gcgagcgcta gaagtgaatg gaatcgtatc gtaccaacat taaaaaaaga
    28621 ttatccattg agtgaagcag attatggttc attggtagcc tattgtttag cctttgctcg
    28681 aatgaaaaca gccgaagccg agataagaaa atcaggtacg tttatcacat gcgaaaacgg
    28741 agtaaagaaa gccaatccag cggttcgggt tcaatcacaa gctatgagtg atttgaaaaa
    28801 acaagccacc tcactaggta tgaccttaga atcacgatca aaactagctt tgaacaaggt
    28861 taaaaatgat gaacccgaag acccgttcaa agagttgatg aattcatgaa tgattacatt
    28921 gagaaagtcc tatcaggtaa gctgattgca cccaagaaaa ttatccaagc gtgtgagcgt
    28981 catataagcg atttggagcg ttctaagtca gatagcttcc cttatgtgtt cgatgaagaa
    29041 caagccacca aagcgattaa attcattgag ttgctaccat ctacggacgg taaagcaatc
    29101 aagatgttag gatttcaaaa attcattcta ggcagtcttt atggctggcg tactaaagaa
    29161 gggaattaca ggcgattcaa tcgagcgttt accagtatga gccgtaagaa tgggaaaacg
    29221 tatatcgcaa gtggcatggc tgccaatgcg ttgattatgg aacaagaacc agcagaagca
    29281 aggcaagtat tgtttgtaag taacgctttg aaacaagcta aattgggcta tgatatgctg
    29341 tctaattcac ttagaaacgt ggtcaagtct agtaagtttt taagaccaca actgaaaatt
    29401 atgaactcta agattcagca cttgccctct aattcgttcg ctatggcact ggctagtgaa
    29461 accagcacgc tagatgggtt tgcaccaaca accgcaattc ttgacgagtg gcacgaagca
    29521 aaaactcgta aaacgtacaa cgtcattaag tcaggaatga cccaacaaaa gaacggctta
    29581 ttgtgtgtta ttagtaccgc tgggcttgat ttaaacgttc ctatgtacga ggaatactta
    29641 ttgttagatc gtgtgctaaa aggcgaagaa caagccgaca ggtactttat agcgatatgg
    29701 gaattggacg atcccgaaga aattcacgat caagagaaat ggatcaaagc caatccgatt
    29761 tttgaaagtg aagaaatcaa aaaagtaatg attccaacca ttcaagatga tgtgaacctt
    29821 gctttgaaac aaaataacct taattctgta ttggtgaaaa acttcaattt atggagacaa
    29881 gcgagtgagg acagctatat gattgctgaa gactggcagg caaccgaagt agaaccacaa
    29941 gatattacag gcaagcccgt ttatatcgga gtggatttat ctaaaacaga tgatttaact
    30001 agcgtttcat ggatcgtacc gctagataac ggcgaacttt attgtgattc acatagcttt
    30061 gtagccacca aatatgggct tcaagacaaa gaaaagcgcg acggtttgcc ttatcgagaa
    30121 ctagaaaaag ctggtgagtg ttccattact caattagaaa gtggaatcgt ggactatgac
    30181 caagtatttc agtttattca agatttgatt caagaaaatg atttggaatg tatggggatt
    30241 tgttatgacc cgtataacgc taattcgctt atcagtaaag ctgaaaaagc caactaccca
    30301 atgttagaag taagacaagg aacgattact ctaaacgttc cgacccgaac ttttagagaa
    30361 caagtttatg aaggcaacgt tattcacaat aaaaatacga ttctcaccca tgcagtgaac
    30421 aacgctattt taaaaacgga taacaacggc attcagatta ataaatcaaa gaacagtaac
    30481 aaaattgatc caatagctgc attaatcaat gcctatgtgt ttgcaatgga ttacttcacc
    30541 acaacggaag gagcgaaagc agacaatgaa ttttatacaa gtgaagaatt ttctttctaa
    30601 ttacattcat accgttcttt tacttcttgg attggtgtgt gtgttggttg ctatcacctt
    30661 actaacaaat gtctactatg gcttgttagc gctgggcata gtgcttattg gggtagcggt
    30721 catgctaaat acagaacaga agggaggtta aaagatggca ttttttaaag cgagacaaaa
    30781 tacaacggga gatcctttcc tacaacatgt ggtatcaatc caatcggatg attacaccac
    30841 cagctttaca agcgttcgtg cattaagaaa tagtgatgtg tttgcagccg ttcgaattat
    30901 tgctagtgat attgcttcaa gtccgattca attggtcaaa aacaatatgc cacaagctga
    30961 taatgaactg gtgaagttac taaacgagaa acctaattca gaaatggatg gttggcattt
    31021 aaagtttgct ttggcggtca atatgttgct aaacggtaat agctttgcag agattaaacg
    31081 caacggtgaa aaggtagaag aaattcactt attacctaac tcaagtgtaa cggttactca
    31141 attagataat ggtacgttgt cttatcagat tggtgataaa aaaagacgtg tgaagtctag
    31201 cgatattttg cacttcaaat atttcaccca agatggttta acaggattac caccacttta
    31261 tgcgttacgt gatgaactga aaattcaaca agctggcaac cgcacattgc acaatttctt
    31321 tactcgtggt gtcagtgggt caggtatttt gaaggttcat aagtcagatt tagacggatc
    31381 agcaaagaac gcaattcgtg aaaagtttga agaagccaac ggttcaagta gtggagataa
    31441 tgctcttaga acgatcattc ttgatgaaac aatggactac aaaacattag aaataaacac
    31501 cgatgttttg aagcttatca attccaatga ttggaacaca aaacaaattg ccaaggcgtt
    31561 tggtgtaccg attgagcgtt taggcgttga aaatgaacat tctagcaccg ttcaaagtaa
    31621 tctccagtat atccaaagta cgctgattca ttactttaat gtgtttgtga gtgaattaga
    31681 tacgaaactt gaaaccaata tccgctttaa ttccgatcag ttactagaga ctgatcctga
    31741 aacaaaagta aaaaatgtat tggatcaggt caaagggtca cttctcacga ttaatgaggg
    31801 gcggtcgaaa atggggctac cccccatgga tggtggcgat cgtttactag caagtttgaa
    31861 ctttacgtat ttagatacgt tggagaaata tcaattaaaa gaacaggaag gagttacacc
    31921 agttgaataa tgaagaagaa aaggaaaaac ggctgacaga agaagctgag ctaacagccg
    31981 attctcccaa agtagagaaa gaaaatgaaa aacaaccaac agacggcaaa actatttcag
    32041 gctatgcgtt gaaattcggg caaccgtcaa aagatttagg cggctttgta gaagtgatta
    32101 cacccgaagc attaaaagag gtggatttat caaatgtgtt cttattatat ggacacgatt
    32161 attcaaagcc actagctagt gtcaaagcag gtacgttgaa attgaatgtc gatgatacag
    32221 gattacattt tgaagcagag ctaaccgaca cgacctattc aaatgatgtg tatgagaata
    32281 tctcaaaagg ggttgttgat tctatgtcct ttgggttcgt gcttggatta gattcattcg
    32341 acaaaaaaga agatggcaca attgaacgat caatagataa aatcaaagca cttaatgaaa
    32401 ttagtgtcgt gaccgttccc gcttatgatt catcaaatgt ccaagtaaat aagcgttctt
    32461 acgaatcgtt tatgagtaac aaccaagcaa aacaaacaaa caatagctta gaatccactt
    32521 ctaaagcaca aaaggagagt aataacatgg aaaaaacttt aatcgataac gaaaaaacag
    32581 aattgcgtgg gtatgaagaa tatatccgtt cacaaggcga agtgcgtgat ggagtcacta
    32641 ctgtaaatgc agcggcagtt gttcccgaag aagtaatcgg tgaagtcttt gatttgaaac
    32701 gttcaaatta taacttagct caatatgcaa cagtaaaaac agtatcaaat ggacaaggta
    32761 aatatccagt agcaactaac caacaagcag tgttagcaac aaaagctgaa cttgctgaaa
    32821 ttggtgatat tgacgctgaa atgtttactt cagttgatta taaagtagaa actcgtgctg
    32881 gtaagattgc cttatcaaat gaggttgtgg aagattcagc agtgaatatt gtacaagagg
    32941 tcaaagatca gttagcgaaa ttggtagaaa acactgacaa taagcatatc atggatttat
    33001 taaaaacatt cactaagaaa acggctgcta cgttggacga tttgaaacaa ctatacaatg
    33061 tggcattaga cccagcatta aataaaatgg taattctaaa ccaaagcgga tattaccacc
    33121 tagatacatt gaaagattca gatggacgtt acattttaca acccgatgtg acagcaccta
    33181 gtggtaaatc attattcggt atgccagtag tattgattgc agatacattg tttgccaatc
    33241 ctaaagctgg aacattccct atgattatgg gggatattgc acaatctatc tttgttgctc
    33301 gtagaaatca agtaacgact caatgggaaa aattcgatta ctactcacaa ggacttgcag
    33361 tgatcgttcg caacgattat aagaaaattg atcaaaatgc ttcagtgtat attgagttta
    33421 cgccagttgt aacaccaaaa gtatagaaaa atattgggcg gcggtttatc ccactgcctt
    33481 tttttcatta ggagagataa aatggtaatt ttggatagca taaaaaaaag tatgcggatc
    33541 gatcacacta ttgatgatga ctttattcaa caattgattg atacagcagg cgaatatata
    33601 aaaagtgcta ttgatagtag cgcaacggat aaagatatgg ataattatca gcaatttgat
    33661 ttggcggtgt cattacttac tcaacattgg tatttgaacc gtcaagaagc cagcagcgaa
    33721 cggataccag taacggtaca agcattagta caacaaatga gaggtgctta ttatgccaat
    33781 cattaagaat gtaaatgaat tgactgagag agtcaagttt aaaaagacaa aacgggtaaa
    33841 agatgaagat gggcagatgg tggatagtga agaaactgta tttgaatgtt gggctaatgt
    33901 gcgttcacaa atgctaaaag acgtgcttgc tagtgtaggt accattcttg aaggaacgtt
    33961 gacgtttatt attcgatacg atcaagatta tgaactaaca aacgatatga aagtagcttg
    34021 gaaaaacaag agttataaaa ttatttcaat caatgaagga acggcgttta aagattatac
    34081 aacgatcata gctaaacaga tttcttagaa tgattacact tgtaaacgtt gtgggtattt
    34141 agtataatta aggtagtaaa tgaagggtta gctgcctgaa ttttgcaaga cttagctagt
    34201 cgaaatctat tgttggactg aaaattgtag gtgtggttgc aaacatgctg gactaagaaa
    34261 gatgatagat gatgaacgtt caattttaat acaggcatgc taggactgat tggatggttt
    34321 tggcaagctg attgtgtaga aatcaaagaa cacgtctgtt gtggacgtgt tttttttgtt
    34381 ataattcaaa agaggtgatt taatggacaa agcagagttt acaaattttt attggaagta
    34441 ttatctacat ttagaaaatg agtttattaa taccacagat tttgtgatgc tcgatgagaa
    34501 taatttcaaa acattctcta ttgaatatca aaaactacta ctagcgattg gttcggagtg
    34561 tgagattatt ttcaaagaac tttgcggatt tgatagcagt agcaataaaa gcataactga
    34621 ttttaaagct gttattaaat ctagcgaatt aatgtgtttg gataatggtg ttagggttat
    34681 gaattcttta acattgactt cactaaaacc ttttggagat gggtggcctg aagatacacc
    34741 agattggtgg aaaatatata acagagtaaa acatggtcgt tcttcaaatt ataaggaagc
    34801 aaatttggaa aatgtgttgt atgctttagc aagtctatat ttattagagg aatatttgca
    34861 taagaaagta atactagaag gtgaagtaga ctttataagt ccagagtcaa atctatttac
    34921 gttatcttgg gaaagaaaac attcgtcgat gcaaaaagtg acgtttgaaa aagtggattc
    34981 aaattatgta gctccactca ttgattttaa agaacaagaa gaaatttaaa aacccccacg
    35041 gcttattgaa gtttttgtat gccatattgt atgccatttg gtttaaatcg cttgaaaaat
    35101 agtgatagat aactctaatt catttagtca taaacgctgt tttgataacc tttatagttc
    35161 tttgatatgg gtttctcatt catggcggaa gaagaagaat agaacaaaag aatgaattaa
    35221 taagatataa aaaaagggat tgtgacaaac gtcttgtcac aatccctttt tccaaataaa
    35281 cgatgttcaa tcagctgccc ctcggcattc gctcgaaaaa cggcgaaata tgaagaacta
    35341 ttttgccatt tgtttctcta ataatcaggt caaaacgttg acttcacagt ctcttcctta
    35401 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag
    35461 ctgttttttg aaattgttcc ttgtgactcg gagctgaacg gcaggctcac gacctctttc
    35521 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtt caaccaaacg aaaattatga
    35581 ggagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    35641 ttttcgttta gcactaaatt tccttaaaaa gtagaaaagc tttcgaagcc tcgtttgttt
    35701 cttttactgt ataataaaag aaaagagtaa caatcgctaa aagatataaa caagagaaag
    35761 gaaatacaag atgattggtc tgatttattt tgtggtgatc gttctggcga acacagttgg
    35821 cgctatttcc gggatgggtg gaggcgtgtt gatcaaaccg attcttgatc ttatcggcgc
    35881 ccattctgtt gcagggattt ctttttactc aacagttgcg gtattcacga tgtccattgt
    35941 ttcgactgtg cgacaagtat caagcgggaa atcactgaat tggcagattg tcggttgggt
    36001 atctagtgga gctgttgttg gaggagtagt cggcaacatt gtattcgaag tttttttaca
    36061 gttatttgaa aacgagaagc atgtgcagct gatccaaatt tttttgacgg tattgacctt
    36121 ggtttttgcg ttcttctaca ccaagcatca tcagccaaaa tttcatttga cgtcatggac
    36181 ttggtacctt atatgtgggg gggttctggg ctttttagct agttttttag gtattggtgg
    36241 tggaccagtc aatgtttctt tgttgatgct gatgtttgca ttgccaataa aagaagcaac
    36301 actatattct ctatctacga ttttcttttc tcaactggca aaattggtaa cgattgcttt
    36361 aacaagtagc tttatgcgct ttgatttatc tatgttgttt tatgttatcc ccgctgctgt
    36421 tattggaggt ttgtggggag caagatttag cagggttttg tctccaaaaa aagtgacctt
    36481 tatttttcaa gtaattgtcc tcgtggttct tttgatcaat ctttacaatg ctttcgtcat
    36541 cttacaaaca ttaggagaat ctagactaac gcaagtcaaa atcactgaaa catattgttc
    36601 taaatgaaaa aaggagtata ctgtgataaa aaaattcatt taaaaaaata aaaattggct
    36661 ggtgatttgg atgttgcggg aaatgcgttt aggagatgca gaagcactac gagaaatcaa
    36721 tgcggaacaa ttaggatatg atgtaccatt aaccgttacg gtgagacaaa tgaagaaact
    36781 gttgaaggag ccagaaaaaa attttttctt ggtctatgaa gatcctactt ctaaaaaagc
    36841 agctggctat gtacaagcgg agtcctatga atcgattttc agcgatccga tgttcaatat
    36901 tatggcactt gctgtgtcta aacatgctga aaaaagagga atcggaaagg ccttgatgac
    36961 tggcgtagaa gaagcgaaaa gacgaggaat atcagcaata agacttaatt ctgcagaata
    37021 cagagtagaa gcacaccgat tttatgaacg catcggttat cattccgata aaatgcaaaa
    37081 aagatttttg aaaatcctgt aaatttttat caaaaaatac ctggtcatca agccgatact
    37141 tgatgatcag gtattttttt tgactaagaa agtaattgca tcaagaaacc tgagatgatc
    37201 aaaacgatgg caccaccaag ccgattgccc atttgagcaa aagcaataag ttccatccga
    37261 ttagctgcag ataagacagc tacatttcct gtaccaccca tcgagttgtt gcataagcca
    37321 gcagtaatag ttgattcgat tggatataaa ccaaacaaac gaccgacaaa tcctgaaact
    37381 actgaaatcg cgataacact tgtcaaacat aagacaacaa attgccatgt caaagcagaa
    37441 gccaatacat ttagattcaa taacgcaata ccgataccgg caagaacagc tgctgtcaag
    37501 tttttgacaa tcaagttgtt gaacatgata gctgcatctt cgtaatattc tggtaatagg
    37561 tttgcgattt tacagataac aacgataatg atcatgaatg cgtaagcatg gactttagga
    37621 acaaaatagt tacagatttg tcctaagatg aagaaagtca atgataccat caaaccgaca
    37681 cctaattgtg gaatggaagg ttttacattt ctaggttttc catcgccaag gtcgcctttt
    37741 tccattaatt taccattacc gttgaatttt ggcattgatt gtcccgcacg agcaactaac
    37801 gctgcaccaa tgatagctag gacatttgtc attgctgtcg caggaatcag gcgggaaatg
    37861 atagaagaag cgtccgtacc taaaacattt gcataaatcg ttgataaagg tacagctccg
    37921 gctccaacac caccagccat tgcgggcaat gagatatata aaacggaatt tgcaaaacca
    37981 tttccgataa gcatacctac gagtcctacg gcaaaaaagg agacgaccat tgaaataagt
    38041 gcaacgggaa taaaacgaac agaagctttc atcaatagat tacggttcat accaaggatg
    38101 ctaccagtaa tcaaggcagc aatataaaag tctaaaaagc ccatattgct gacaaagttt
    38161 tctgtggatt ttactacata ctcaggtaag atcccaaatg tagcaatcgc ggcagaggca
    38221 aagatcgcaa aaacggagcc accacctaaa taacttttga cgatagggat ttttgttccg
    38281 ataaaatgaa ataaattacc gaaaatgacc aaaacagcga ttgggccaat catattcgtc
    38341 ggtaattttc ctaaagccat tgcaatgatc aggacaagga ccatgatcaa ataaaggggc
    38401 aaacttacgc cggtaatttt tgttgaccaa aacccttctt ttttcttttc ctcaactgca
    38461 gttgcggttg ttgttttttg tgccattttt acagatcctt tcttattctg tatcgtatga
    38521 aaaaagccaa agagaagcgg caaaagagca gtagttggct ggctgctgtt ttgccgcagt
    38581 cgcttttttc tttttagacg tcatacaaag tttctgtatg ttcttttact ataacaatct
    38641 taatcttcta aagatttata ttcaggaacc catttagcag attcaacagc ttctttgata
    38701 tccgtgattt cttcacgatt caatttttgt gcaacaactg attttgcgac agtttcagca
    38761 atcgtttgag agaattcagt gatttttgct actggaggca agatagctgc tcctggtttt
    38821 gttacatcaa cgattccgcc taatgctcga ctagcttgag aaatgatttc agcatttact
    38881 cgtgtagctg ttgaagcaat caaaccaagc cctaaacctg ggtacatcaa cgcattattt
    38941 gcttgaccga tttggtaagt tacaccgtta tattccacat cagcagcagg gatacctgtt
    39001 ccaactaaag cttttccgtc cgtccattca atcaaatctt ttgcttttgc ttctgccaat
    39061 tttgttggat tagacaaagg catgatgatt ggacgtgggg tatgagccgc catttctttc
    39121 acgatttctt cagtgaatgc tccaggttgg gtagaagtac cgatcatgat cgttggatgg
    39181 attgctttta caactgcttc taagtttgtc aattcatcag cattagtgaa ttctgagcgt
    39241 ttacgagcga atgggatttg accaggagtc aatccttttg tatcttcaaa caataaaccc
    39301 tgtttatcta cttgatagaa atgttggcgt gcttcttctt caggtacacc ttgacggatc
    39361 atttcatcta aaaggatgtt tgcgatacct acaccagcag taccagcacc aaatgtcaag
    39421 atcgtttgat ctttcaattg ctcacctgat atattcaatc cacctaatac accagctagt
    39481 acaacgattc ctgtgccttg gatatcatca ttgaaagtag tgattttatc ttcgtatttt
    39541 tctaagatcg ttgcagcatt tcctcgtcca aaatcttccc aatgtaataa caattctggg
    39601 aataattcag tagctgattg aacgaattga tcaatgaatt cgtagtaagt ttcgccttct
    39661 acacgagcat gacgattccc taagtataat ggattattca ataattcttc gttattagta
    39721 ccagcgtcga ttgatacagg caagacttgt gcaggattga taccagccgc agcagtgtag
    39781 accatcaatt taccgatagc gatgtcaaca ccgttcacac cccagtcacc catacctaag
    39841 attccttctg catctgtcac aacgatcaaa cgaatgtcgc gtccatctgc tgcgtttttc
    39901 aaacttgctt tgatattttc tggttcgtcg attgaaagaa atgcagcatc ttgtggtttc
    39961 aagaaaattt cgttgtattg ttcgattgaa tccgcaacga caggatcata aacgacaggc
    40021 atgaattcaa ctaaatgttc gcccatcaat ttataaaaca atgtccggtt cgtattgaat
    40081 agattcatta agaagatacg tttttccaaa tcggtctgtt tgcttaagta ttgaccgtaa
    40141 gcttggacag attgttgttc caatgtttgg acagtacttg gaagcatacc agtgattccg
    40201 tattttgccc gttcttcttt tgtaaaagct gttcctttat ttagaaaagg attatttaat
    40261 aagtttagcc ctgtaagcat gaatgattcc tcctaagttt taatacaaac gaagtatagg
    40321 agtcagatga ggatatagtc aaaaagagca ggtataataa attttattta tatgagtaat
    40381 agagtcaaaa gaaatcaaaa tagcggtatt tgttcttttt atatcaacaa tccttgcttg
    40441 attactatgc gtatctctta tatgctaaag aaagaaaagt agaagaataa aggatatgtt
    40501 agatagataa atagaatctc tgaagaataa atcacgtgat cgtgacgata aaatacactt
    40561 acgtcaggtg ataaaaaata cataagtaga aaactaagag gaggaacaga aaatgaaaga
    40621 agttcaattg atcagaaaaa gcgaactatc agaaggtggt tgcaatgcat gcggcgtagt
    40681 agaagcaaca agctatacat taaagcttgg tgccaacaaa gcgataatct ctgagttgac
    40741 agtaggcggt cttgtggaca gtctggcgct tgctgaaggt tttattggag aagatattta
    40801 tgagatgttt tcagaaatcc gtcagttaaa aaaaggggaa aactgcatag aggttcatca
    40861 tgagagcccg aatgtgagat tcaaacgtgg tgataatgag atgatcttca ataatcatgt
    40921 gtccgatcac accgagctat atggaatcgt gaatcaaata ctaactgagc tttttggatt
    40981 gggaccttat gcgttcaagg aagaaaacgg taatccaaaa ttaaacgaag aatggcaaga
    41041 aacgattgaa atacaaagaa acaacccgca cttatttcaa taggcatcca cagtatttcg
    41101 taaaagggac tttgacacga cttttgtcaa agtcccatcg ttttttatct atttgtgttc
    41161 taaacatttc tttcagagta gtaacaactg gaatgactga aatgtaaaca gattatttgc
    41221 ctgtatcaat acggaagagt aatataaagt gtgtaaacgc tatcatatta ttcattcaat
    41281 tttttataga gggaaggcat tgaaatggaa aaaacgtatc aagcaggtac aaatgaagga
    41341 atagtggatt ttatcaatat ggaagattta gaaatagccg cttcacaagt gattccagct
    41401 ggtggttatg gttacatcag cagtggtgct ggcgacttat ttacttatca agaaaatgaa
    41461 cgggcattca atcatcagct gattattcct catgtattaa aagatgtaga attacccgat
    41521 acaactacac attttgacaa agaaacgctg actgcaccaa tcatcatggc accagttgct
    41581 gctcatggct tagctcatgt gaaggcggag aaagcttctg caaaaggtgt agcagatttt
    41641 gggacgatct atacagcaag ttcttacgct tcttgtacat tagaagagat aagagaagca
    41701 ggcggggaga aagcaccgca atggtttcaa ttttatatga gcaaagatga tgggatcaat
    41761 ttggatattc ttgaagtggc caagcgtaat ggagcaaaag caatcgtatt gacagcggat
    41821 gcaacagttg gcggaaaccg tgaaacagac cgcagaaatg ggtttacctt cccactgcca
    41881 atgccgatcg ttcaggctta tcaatcagga gtaggccaaa cgatggatgc tgtgtacaaa
    41941 tcttccaaac agaagcttag tccaaaagat gtagaattta ttgctgctca ttctgatttg
    42001 ccagtctatg tgaaaggtgt ccagtcagaa gaagatgtct atcgttcatt agaatctggt
    42061 gctggcggaa tctgggtatc taatcatggt ggccgtcaac tagatggtgg tccagcagcg
    42121 tttgattcgt tgcaatatgt ggcagaagca gtagataaac gtgtaccaat cgtgtttgac
    42181 agcggtgtac gacgcggaca gcatgttttc aaagcaatcg catcaggtgc agatcttgtt
    42241 gctattggca gacccgttat ttatggattg tctttaggag gaagtacagg agtccatcaa
    42301 gtgtttgatt tctttaaaac agaattggaa atggtcatgc aattagctgg aacacaaaca
    42361 gtagaagata tcaaaaaaat aaaattgcgt gagaatcgtt ttatttaaga aacaaatgac
    42421 gaaaaaaata ggagtgcggc agatgatgcc gtactcctat ttttatagtt caaggatgtc
    42481 actaatagaa taatttgaaa cgaaaaaata aaattgaaat attttttcat acttaataaa
    42541 aaaggaagaa tattcatgta aacgatttta ttgttttttt ctcttttata caaaaagggt
    42601 cataatttca agtaagcgga tgaattcatg aggatttttt gcatactgtc tctactatac
    42661 ttaggtcact aagaaaaagg agtgatttat tatgaaaaag aacatgattg ggacattatt
    42721 actttcaaca cttcttctag gcggcttggc aacaccagct tttgctgagg gtcaagcgac
    42781 ttcaaaagga gatattacct ttactgaacc aacaaatacc gtggagcctc ttaaccctac
    42841 tgatccttca aaaccagtag agccagcaga tccggaaaat ccagcaactg gccagacagg
    42901 tcctttaaca ctggatgtag ttcccgaatt gccatttggt acacacgaga ttgaaagcgg
    42961 aacaaaaacg tatcaagtag acgcttcaaa aaacgataca ccttatctcc aagtatcaga
    43021 tcgtcgaggt gtaggtgcag atggccaagc acaagggtgg aacgtgacag tttcggtttc
    43081 agattttgta aacggttcac aagttttgca aggcgctgaa ttagattttg gaacatccac
    43141 tgttaaaagt acgtcagata acgaatccac tggaccaact agtcaaactg taactgggtt
    43201 gagtaaagca agtgcagcga caccaatatt tacagcagca aaagatcaag gtctgggaac
    43261 atggctttct gtttatgatc cagcaaatat cacactgaaa gtgccgaaag cagcagctgg
    43321 aacattcacg gctgatttga cttggaacct cgtggctggc ccagtagcat agagtatgaa
    43381 ataaattcaa caaactgcgg tgaaaatact ataggtgaag gattttttac cgcagttttt
    43441 gttgaaagga tgtggtggac aatgaatcaa agtaaacgac tgtttgtttc gtttttttct
    43501 attttaagtc tatttttcat ttttccgtca atcagccaag caaaggattc agcaggagac
    43561 tttggaatca aaccggtatt tccagagaac caaatagata aagcaatcgg ttattttgat
    43621 ttattagtgg caccggaaca aaatcagaca ctagaagtaa ttatcagcaa ttcttcagat
    43681 gaagaacgaa catttgaggt ctctgtcaat ccggcggtaa cgagtgatgg tggaacgatc
    43741 gattatagtc aaaaaaaccc aacgttagat gagacattgc cgtttgatgt ccgtgacgtt
    43801 ttgctcattg cgaaaaagga aatcaatgta tcagcacatg cagaaacaac tgtacctata
    43861 gaagtgaaaa tcccagcaaa aagctttaag ggacgtgtat tagcgggtat tcatgtttca
    43921 cctaaagaag aagctgaaac agaaaatgct acagaaggag cacaaatcaa aaatcgtatt
    43981 gcttataatc tcgcagtcgt tttacaagaa agtcaagaga cgatagaacc ggatctgaaa
    44041 cttttatcag gagatttgga cgaggtcaac gcaaagccaa ctgttcagct tcgtttccaa
    44101 aatcctcagc ctaggattat cagcaatttg atatttacga gcaagatctt ttatgaaaat
    44161 cagctttata ttgaaaatac gtctaatgca tttttagttg caccaaattc gaattttcac
    44221 ttgaatcttg atttagctgg tgataaagca aaagccggcg attaccgcgc agaaatcata
    44281 gcaaaatcag gcgacagtaa tgaatggcga tttacacaaa actttacgat caaaaaagaa
    44341 aaagcacaaa aagtaaatga aaattccgtt tttgcagtac aagaacaatc tttcccatgg
    44401 ctttatgcag gattggttgc tgcagttcta gtgttatggt tgattttgct gtttttgtgg
    44461 gtccacaaga aaaagaaaaa aggagaaaca acatgatgag gtgatttcgt tgaaaacatc
    44521 gatcttattg ggagggatta ttgcaagttt gctgttcttc agctggcaag agccagtcca
    44581 agcagatacc aaaagttcag gtacgattac attttatgat ctaagtccaa ttccgcatcc
    44641 aagtggttca gtcaatggcg catcaaaaca gcagacacag ctaccgattt ataaagaatc
    44701 taatcaacat ggcgtattcc cggagacgaa tgaacaaaag atccgttttc tttatttgat
    44761 cattggtata gaattatggc ttcttgcgtg gttgttgtta tggagaagaa aggaaaagga
    44821 ggatgaacat gttgctacaa aataaatttt cattttttcg ctccttcttt tttacttcgc
    44881 tttttgtttt aagtatattt atcttttcac cttcggcaga agctgttgga gcaacgcctc
    44941 ctgccatttc ttatgatcca tacacgtatg aaaatatcgt acctccaaat ggtggacgta
    45001 gtgcagtcaa tggcgggaag cttgtccaac taactggggc taaccaattg cctaaattga
    45061 ctgctactta tttgaacgat ggttcaaatg atccttggaa aggtggcacg gtccgttttc
    45121 attgggaaag ccatgtgaaa ctaacaaatg tacttgattt ggtgaatatc actgcacaag
    45181 gatttgcatt tgtggcgatc atcaatttgc cggaacatgt atcaagcagt gatgtattaa
    45241 aagcaattga ctggaacaac gcctacttgt atattgccgg tgcccccatc aaggttacta
    45301 gcggaagttt attatcagca ggaagccata ccttgcgttt tgggatgggg tcgtgggata
    45361 caaatactct attgacagat attattgcta ttattacgcg aggcgaattc aacgtggacg
    45421 atatcccgct tcaatttgat gttacagcta atgtcgcgaa gatgacagaa aacggagata
    45481 aatatgatac gagtccaaac aaaattttga ccaagggtaa attacaacct tctatgtcta
    45541 aaaaggccgt tttttctgtt gatttttatg attcgaataa tattatccaa gatcgtatgt
    45601 tcaattcgat tttagctcca gctgcaggta gattttatcc taaattaact ggcggaaact
    45661 tgaatccagg agcatatgca ttttctgcaa gtgcagagat aaatacgtgg aatagttaca
    45721 tctcaccaag tgattcaaca ggaaaatata atacatttgc tgataccaca gaatacgtgg
    45781 atggaaataa ccagaacctt ccaggctcta ccaatagtcg gacattgacg atggggctga
    45841 ctgaaggacg ttttgatcag ttgccattta gtcgctttga tcgcgtagtc aactatttta
    45901 caggaaaaaa cgttacacaa ggagcaatac tgactcatac tccagcaagc acaagtggat
    45961 tgaatcagac agccaatatc gtgtacagtg ggaaagatgc gcaaggcgga ttgctttctc
    46021 ctgttgcttt aaaggtattg gagcagtaca agctagacgg aacggtttta ccaacaaact
    46081 taacagccga tagtcagtgg ggaaagctcc tttcttctgc atcgtaccaa gtcacggcca
    46141 attggtcagc aactgaacta aaaaagggtt cgatccatta caggctttat tcgaaaggaa
    46201 cccaaaaatt agcgacagga tggacagatc aacttttcca aacgatccaa tcaaataacg
    46261 ggggtgtaaa tacagctact tctacactac ctgcattggc ttcagggcaa tattattttg
    46321 attatcgctt ggtggacgat gtacttacta acatgtatcc gtcttttgcg tacaaatggc
    46381 agtctgaaca agctggaatc accactcttc ctcaaataac agtggctaat tttccatctg
    46441 tgagtgcaga tagtcagcta aaaaacctta gcagacaacc tgagacagga gatcccctgt
    46501 ctgctctatc aggagatacg attcaagaaa atgtgacatt cacgttaaca aaatctgggg
    46561 atgcgataac cgacaaaaaa atcacgatct cacttcctga aaatactact tatatcaatg
    46621 gttccttaaa attaaatggt acaactatcg cagataccgg cattcaacaa ggagtttctg
    46681 tcccagcaga tctattgagc aaaataggag ataccattca tcttacttac aactatcaat
    46741 taaatactgt agatacttct ttacaatccg tcagcatact gacaaaggca gctgtacttt
    46801 ccagcaatat tacattagca gatggtgcta agcttcctaa cccagttgtc caaacatctg
    46861 ctaaaacaat tcttgtcccc aaacaagaat taacattagt taatgtacca gatgatttta
    46921 catttggtaa cgatctcccc aaaccgttga agacaagtta ttatgaatca aaaggcgatt
    46981 tttcttttga tgtgcgggat actcggcttc caagcacgtc tccttggcaa ctgacaggta
    47041 cattgacttc tttatttaag aatgatcaag gtcaagaatt aagcggaaca aaattatact
    47101 tcaatcattc tggatcaaag caattgattc agcaaggtca aaacacgttg atttatgaga
    47161 gtgatggaac agctaaagga gaggttctcg ttgactttcc cgataccgat ggtttactct
    47221 tagaggtaaa tagcagtacg aatgctcaac ctggcgcaac gtatcaaggc atggtcacat
    47281 ggaaactgac agctgggcca accagctaag tgattagaat ttgtatggat ctgtgataac
    47341 tgtcacagat cctctttttc aaggatgaga aggaaacttc tgctgttaaa atcagaaatg
    47401 cttgaaggag tggacttgga acttttcagt ttgcttgaaa aaaatgaaca acggcaaatc
    47461 aaacttttcc aacttttatt agaggaaaaa ggagaggttt ccgttaataa cgtacttacc
    47521 cgcttatcag ttgatcgggc taccttgaaa gaagatctgt tttatttaca agctaacttg
    47581 gaaaatatca aacaattgga aattgttatc caagaaggga atattttatt aatcagacat
    47641 ggaaatatag cagcagtcga tgtttattat cattgttact tgcgctattc tacaaaatat
    47701 cagatacttc tttatctgtt ggaaaatggc tcatttgatc ggcagaaact tgtcatggaa
    47761 cttaatatca gttcggctac tttgtctcgc agagtgaagg aattaaatga ttctttaaat
    47821 gagtttcatc tgaaaataaa aagcgggcgg ctgaccggtc cagaaagtca aattcgttat
    47881 ttttatttcc aattattttg gtttggacgt ccctatcttg aaaacaaaaa agaatacagt
    47941 gatttatcag gaaacacttt tttagatctg ctgcaaaaag agtttagttt tgctattaca
    48001 gaagaaggac gattgaaatt tgaaatatgg ctttccatta tgaagaagcg aatgcgctgt
    48061 acgaaaaaat ccaaaccaga ttatagcaat cttcctgttg gattgtttca aacgacacaa
    48121 tttttattgg acctccatac atttttgacc cgttacttct ttcaatatgc ctttccttgg
    48181 aatcaaaatg aaacgttggt tttttatctt ttcatgatca gtaactttac attagataca
    48241 cataactcgc atgtccagga gtttttgata agcgaaaaat acaaagacga cgtagtagct
    48301 caattgaatc gaacgtttaa acaatcgata gaagaatatt tctcttttta tgtttttcca
    48361 gaagagattt cgcaaaagat tgcgttgaca gtgatgcaaa atcacgctca ttttgcctat
    48421 tttagaggat ggatgaatat ttttggccaa gaaggattaa aacagcggtt aactgaagta
    48481 gaagataaac aattcctggt tttatgtgaa agattggtca gacaaacatt tgctattgtt
    48541 gattttactg aagtagacga ggaaaatgcc cgctttgaat tatttgctcg ctatgcatct
    48601 attttacgga cgctgatcaa gcagttagaa ttacgtctgc aaattgctta tgatcttcct
    48661 tatgaaaggc taatggcgga tatcgtgaaa gaaatcatcc aggaacaatt gctgaatacg
    48721 atcaaatatg atttagtcga atacgagaaa gaaaagcagt atgatatcat tttgactagc
    48781 caattgaaag agtacccgtc acagaagtcc gctaaagttt ttgtattcac tagcaacgaa
    48841 tataattatg atttccccta tttgaaccaa tttttaaaag aatgttattt agaaaaattg
    48901 aatttaagga tgaataaaac atgaaaatcg tgatcattgg tgcttcccat gcaggcatca
    48961 cagctgccct aaatctaaga aaacttcaac cgaaagcaga agttttactg atcgatgaag
    49021 atcataaaga tggactaggc tatgtttcaa atggaatcaa cctttattta aaaggaaaga
    49081 tacgtagttt agctgaagtt gcccataaca tgcggactct tcaagaaagt ggggcaaaac
    49141 tgatcaccga atggagagta actgaacttg atccagacaa acatcagctc atcctcgcat
    49201 ccaaagaagg aaaaaacgag accataacct atgataaatt aatcgtagcg acaggttcct
    49261 ctcctgtcac cttgtataaa caaattgaag ctgaaaatgt ctacacctat aaaaatctag
    49321 tgcaatctaa gcaagtactt gccgctttaa aagaagcaaa agaagtagtg atcttcggtg
    49381 ctggttacat tggattggaa ttagcagatg ctttgagaaa taaaggatac attattcatc
    49441 tagttgatta tatgccgaat gttttatcaa gatatttcga taaggatatg atcaacagtt
    49501 ttcaaaatca gctgcaaact aagcaaataa atttttatcc aaatgaattc ttgatcgatt
    49561 ggaaaaaatc agaagagaaa gtagtatccg tacaacttct gtctcaaaca ataaaggcag
    49621 atatggtcat tttttctgcc caaactcgtc ctaatacgac attgttaaaa gaaaaagtgg
    49681 ctttgtatga ggatgagact gtgatagtca atgaatatct gcagacaagt gatccggaca
    49741 tctatgcgat tggagatatc gtcccagtca gttttgacaa aaataaacgg catctctttt
    49801 tacctttagt tactcgagct gtacatatgg caagagcagt agcgctaact ttgagtggtc
    49861 agccaaccgc ttacgacctc agacaaaaga taacagcagc agtcattata gactactttt
    49921 taggaactgt agggctgaca gaagatgaag cgccattctt ggaacaaaat accggttcgt
    49981 gttcaggaga atttgatcta tttccacaat acgacgaaga aaacaaaaca gtaaatgcaa
    50041 agctgatcta ccatccggat acattagaaa taattggcgg acagctgata agccaagaat
    50101 tcttattatc agatttgaac ctgcttgcgg atatcgtgaa acacaaaact acgattccac
    50161 agctagcagt tgaggatttt ggatttctgt ctgaatacac ctctcgtttt cactacctca
    50221 atgagttagc ttttaaagta ctagctaaga gagcaaatag caatagagta gataaaagac
    50281 tgtgagaaat atcacggtct tttattgtgt ggggatataa taaatattaa tcaatcgaga
    50341 ataaaaaatt catataatga aatagcggaa gaattttttg agcataatat tcgccaaaaa
    50401 tttatcaagt gataaaattg ttgtataaaa tgattatttc aggaggagga gttaaatgtt
    50461 tttagcatta aatgaaatta tgcattcaaa gttacgttat gcgttagttg caggggtgat
    50521 gtttttgatc gcctacctgg tatttttttt gactgggtta gcttatggct tagctcaaga
    50581 taatcgaaca gcagtcgaca agtgggaggc agattcgatt gtgctttcga aagatgcaaa
    50641 ttcaaatcta gggatgtcca tgattactaa gaagatagca gaagaagtcg aaggtgggaa
    50701 agtggcgtat cttgcccaaa cacctggagt cgtgactagt aaagacagta cagaagaagg
    50761 aaaaatcaat gttagcttct tcggtatcga taaaaatcaa ttcatcatgc ccaatcttgt
    50821 tgaaggaaaa gcttttgata acgatgatga ggcagtaggc gatattagtt tgaaagaaga
    50881 atatggtcta gccataggag atacagttaa gttatctggt agtgacaaga cgtttaaact
    50941 aactggcttt acggatcatg ccaaattcaa tgtctcacca gttctctata caaccatcaa
    51001 tgcttaccaa gaaatccgct tcgaaaaaga agatacaagt gaaaatgcac ggattaacgc
    51061 aatagttgtc agaggtaaaa tcagtaattt acctgaagat ttagaacaga tcaaaatttc
    51121 taaattcatc aatgaattac ctggatataa tgcgcaagta ttgacttttg gatttatgat
    51181 cggatttttg atcgttattg cggctattgt gatcgggatt ttcatttatg tactgacgat
    51241 gcaaaagatt aatatctttg gtgtaatgaa agcacaaggg atcacaggag gatttattgc
    51301 gcgttctgta gtggcacaaa cattcatttt atcatttgtt ggtattcttc ttggtctatt
    51361 aggaacagtg ggaacatccc ttgttctacc tgatgcggtt ccattccaaa gcaattggct
    51421 gttttttggc gtcattagct tgttgatgtt agtagtagct gtgttaggcg cactattttc
    51481 tgttcggaca atcgtaaaaa tcgatccgtt gaaggcaatt ggataagaag gaggaagaac
    51541 atgaaagcaa tagaatttaa agaagtagaa aaaagtttcc aagatggcga ccaagtagtt
    51601 aaagcattga aagaaacaac catgagtatc gataaagggg aatttgttgc aatcatcggt
    51661 ccttctggtt caggtaaaag tacttttttg actttggcag gcggattgca gacgcctagc
    51721 agtggctctg tatttatcaa tgatcaagaa ttcagtaatc aaaaagaaag cgaacgcgtt
    51781 aaactacgtt ttaaagaaat tggctttatt ttgcaagctt ccaatctggt tccttttctg
    51841 acagtaaaaa agcagttatt attagtagat aagattaaac atgaacatcg tcaaaaagaa
    51901 gcaaacgaat tatttgagca gcttggcgtg gataagttga tcaacaagta tccagaagaa
    51961 ctatctggtg gagaacggca aagggtcgca atcgctcgtg cattgtacaa tgatccgtcc
    52021 attattctag cagatgaacc aactgctagt ttggattcag aaaaggcacg agaagtagtc
    52081 gatatcttgg caaaagaatc aaaagacaaa aataaagcaa cgatcatggt tactcacgat
    52141 acacggctga ttgacaaatg cgatagaata tttatgatgg aagatggtgt attgaaagaa
    52201 aaagaataac caaaaagcag gtggaaaaat ggtccatcga tacaacaaag atacacagca
    52261 aaaaattttg aaagaagcga ctgacttgtt tatgtccaag ggatatctcg gcacttcaac
    52321 aagagaaatc gcacaaaaag ttgggatcac ccagcccaat ctctatcatt atttcggtga
    52381 taaagaaaaa ctatatacgg cagttttaga aaaccactta aaagatgttg gaaaagcttt
    52441 gagagaaatc gttcaaacaa gcgaaacggg atttcaagta accttgacaa aaatggcgca
    52501 gtttttgatt gagacacatc tggtcgattt gtttctgatg cttcacgatc ttgaaagcaa
    52561 tctttcaaag gaaacaagag atcatctttt ctttctatgg aagaaaaatt atcgtgaacc
    52621 ttttgaagaa atcttttcag caaaccaatc tgtgctaaga aatgggattt caaaagaaat
    52681 cgcagcaagg cattttttct tagtgttagc accttacatc actcggtcga aaagacctgt
    52741 ggaagaaccg ctaaatgtgg cgcaattgat cgatttatat ttgaatggtg tattaggaga
    52801 agcgtaaagc tgttatatag ggagaaaata gttaggtgaa gaagcaagat atgacgaaga
    52861 tcgttatatc ttgcttcttt atataggtag tttttcataa agacaaattt gttattctct
    52921 catgattatc agggtatatt tttatttatt atgtcggtgt tccaggaagt gttttaattt
    52981 aataacgaag aaattaattg tttgtagaat gaaggaaact atttattggg tagatgaatc
    53041 accacgaata gtcaaaagga gatttcagga tatgccaaaa atgaaaaaaa tcattttagc
    53101 agtaatgtta ttttctacgg ttatatcttt tacagcccct tttttgctta cttaagctga
    53161 ttttaagttt tggcccttag tctggatcta cgaggtgaga gtcaagaatg aaaaaaataa
    53221 ttggagcttt tgtatttgca atcattatca ttattatctg ttttcaaata gatgtttttg
    53281 gtcaaaaaaa gatagttgga caaacggtca gcagtacgaa cagtacggtt acaaagacaa
    53341 aggaatcatt tgatgataca gaacaagaga cattaatcaa aatcggaaaa aatgaccgta
    53401 cactatatac agatgactcg ctaactaccc cacttgcaac tataaacggc ggagagctgg
    53461 cggagtttct ttctgaaaca aaaaatgctt atcagatcaa gacaaatgat ggctatactg
    53521 gatatttagc cctcgaagat ggaaccaaag tcacaaagaa tatacaaaca aaaccggaag
    53581 agctatctga tgcagtgatt gtattagatc ctgggcatgg cggaaatgat actggggcat
    53641 taagcaatga tgaacaaaca gaagaaaaag atataacatt aagtacagcc ataaaagtaa
    53701 aaaaggcgct agaggatgct ggagcaacgg tttatttgac ccatactaca gatgaattag
    53761 tacagcttgg agatatatgc gactacagtg aagaaaaaaa ggcagatgtc tttatcagtt
    53821 tacacgcaga ctctacggaa tatgcgaatg aagcaactgg aatcacgaca tattattact
    53881 atggacaaga ggaaacatta gcacagacaa ttgctgactc cttcactgat ctgcctctgg
    53941 attcaagagg aatctccact gggaactatc aagttcttcg agaaaatctt caaccttcta
    54001 ttttaatcga gatgggttat atgaataatg atagtgattt aaaggaatta gtaacagaca
    54061 gttatcagca gcagattgct gcaagtttga cacaagcatt gaccacttat tttcagtaat
    54121 aaataagccc agccaaacaa aaatagctcc tcatattttt cgtttggccg ggcttattat
    54181 cgcagatgca agcttttgaa caccgtttat ttttttagta gcttcaaagc tttttctgta
    54241 tttgcaaagt gaagtttggc atatttttca agcataggca tgccgccttt ctccaatagt
    54301 tttgccgcta cttggtcaga tttcgttcca gctcctgatg gaagggtgat tccagcttca
    54361 gctgatgctt tgtgtaactc ttccaaaaag ctggcacgac tgatgataga agcagcagca
    54421 acagctaaat gatattgttc tcctttagta acgaagtaca gtttttcttg taattgattt
    54481 ttttctaaac gcgcatattt tttaaaattt gcttccggcg taaactgatc gatcaaaatc
    54541 gcttctggtt tttctggctc gatttttttt agcagcaaat ggatcgcttg attgtgcaaa
    54601 gcgactttca tccgaacggc attgtatttt ggttggatct cattgtattt ttctggagat
    54661 aagacaagca gtttataggg gatggttttt tttaagatgt cagcaagctg gatgatttgg
    54721 ctatctttta actcttttga atctctaaca ccaagtttac gtaattctgc aagctgatca
    54781 cgtttcacat aagcagcaca tactgttagc ggaccgaaat agctgccatt tccgacctca
    54841 tcactaccta aaacggataa ttgactgata ttttttggca aagaagaggc tgtttttgct
    54901 ggcttggctt gtttatcctg tgcctccccc catttatcag cttctattgc tgcattcgta
    54961 ccttgaaaca tcacttttcc ggatgtataa gcggtaatgg tcgtcccgtc tttttttgca
    55021 gcaaagctag tataaggaat ctgtttattt aacaaataag agcgatagta tgctttcatt
    55081 tgactaagtt ggtcgtttgt tgctttgata atgacgttgc tcataaaatt ctcctcgttt
    55141 ttatacataa aactataccg caactcacga cttttcgttt tctaagagat tgcgagacat
    55201 agcatttttt taaagtttat catataatag ttaagtatgt gaaaaatatt tgtggagaac
    55261 ggtgaaagaa ccaacttcat ggtacactaa ataaaacttt catcaaatag gaggaaatga
    55321 ttcgtatggc gcacgaaaaa acacgctaca aagcggtgat tgccaaccaa acatatacta
    55381 ttatcggacg ggaaaccaag catcatatgg atatcgtaac gaagctgatc aatgaacagt
    55441 tagcagaatt gaaacaatta tcaccacaaa tggataatga acaagcagca atcttgatgg
    55501 ctgtcaatgc gctttcggat caattgaaga aacaagaaag aatcttggag ctggaagaag
    55561 aaacagcaga attaaagaaa aaaatgatca aatttacaga gcttgaaaat cgtgtgaaac
    55621 gcattgaagc aatcgagaac gaagctcggg aagtattgaa agaaaatggt caagcagatc
    55681 ataccattaa aaatcatgtg gaagcacaac agattttaaa cgagaaacga aaaggacaaa
    55741 ttaaacaaaa atcttctaat taagatcctt ttatcaaaat aagaaaaatt taaaaaaagt
    55801 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    55861 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    55921 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    55981 attccatacc cagctgtcac gccaacatca aagttagcat tttttgatca ggtatttgcc
    56041 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    56101 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    56161 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    56221 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    56281 gagcatagtt tcgctcgctt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    56341 gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat atttcgtttt
    56401 ttgtacagag tagattattt aagaaggctg cgtagcgtat gtccggcaca gctctttttt
    56461 catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa
    56521 aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga agaactggca
    56581 gaattggtac cagtgagcga ggaacaaacg atcacaaact ggcttcaaga aactgaggat
    56641 ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaacttga aaatatccgt
    56701 ccccatatga aacggattga gatcggagca gacttgaatg gagtggagct tgcacaagtt
    56761 tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct ttatgacagt
    56821 gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat ccctgttttg
    56881 agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga cgcttctcct
    56941 gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg tgaacagtta
    57001 gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat caccatgcga
    57061 aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc
    57121 cacgatcaaa gtgcttcagg acagacttta tttattgaac caaaacaagt ggtggatttg
    57181 aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg tattttaagt
    57241 gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatgc ttatgtgatt
    57301 ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa agctatcgtg
    57361 ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc actgatcgat
    57421 caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc catcgtcatt
    57481 acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct gttacaactg
    57541 atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag
    57601 gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc gaccttttct
    57661 tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag tctggtatta
    57721 tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc aatcgcaatc
    57781 ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta tcctgaattg
    57841 aaagtttatg gatataatcg ggcaaatacg attaatgcca gcatggagtt tgatgtggat
    57901 acattaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa
    57961 atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca gttgatgaat
    58021 gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg gaaaatggca
    58081 gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact tcacgatgat
    58141 ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag
    58201 aaaaaagcaa atgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat
    58261 attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca gttgatcgat
    58321 gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa taaagtattg
    58381 aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt tacgacttac
    58441 ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga aatcggtata
    58501 ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa agagccgaca
    58561 caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa tcaattggac
    58621 cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt ggattctgcc
    58681 attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg cgcacttcgc
    58741 aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga gtttgcaccg
    58801 gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt actaaaggta
    58861 agcagatggt tcgctatcca agtatagaaa tgctatacta gtcatagatt ttaaaaatgg
    58921 aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa acagataaag
    58981 gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga
    59041 ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct aaaatggatg
    59101 tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca acattaatgt
    59161 tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac
    59221 gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga gggtgggact
    59281 gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt
    59341 tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc tataataact
    59401 ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta
    59461 cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc
    59521 ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggtag ccataatacg
    59581 aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat
    59641 atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt
    59701 atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt
    59761 ttggtgatta ctcgtaaggt actaaaagac aaagcactgt attttggacc atatcccgat
    59821 gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc
    59881 aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg tctgtgtcct
    59941 tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaacttt
    60001 ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct
    60061 gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca
    60121 atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat
    60181 gcgattgata aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt
    60241 gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac
    60301 ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat
    60361 acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga
    60421 ggagaaaaga aaaaattagt tgaattggcg agtaagaatg cctcagttgc tttgaatgaa
    60481 cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga
    60541 aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg
    60601 gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa
    60661 tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa
    60721 gtcatctatc gcagatattc gcgagtgatt cgcgaagggt tgcctttacc tgatttgatc
    60781 ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta
    60841 ggaatcgata ttcctattgc tggcatggca aaaaacgata aacataaaac aaatgagttg
    60901 ctattcggta cagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta
    60961 ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc
    61021 agtaaaaaca gttttgcttc ccgattagat gaaattgaag ggttaggacc aaaacgaaaa
    61081 aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa
    61141 ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag
    61201 gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa
    61261 aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata
    61321 tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact
    61381 tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg
    61441 gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt
    61501 ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat
    61561 gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac
    61621 caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc
    61681 gacttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg
    61741 tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg
    61801 atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat
    61861 ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat
    61921 aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat
    61981 ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt
    62041 ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac
    62101 aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg
    62161 actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc
    62221 aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt
    62281 aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg
    62341 tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt
    62401 attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga
    62461 gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga
    62521 tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa
    62581 ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat
    62641 gcttcgagcg agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc
    62701 aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag tcggagaaga
    62761 taactcgaca gtccaatacg tcgtaagtta tgtagcaacg attgtcttgt tcattattat
    62821 tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat
    62881 ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaat taacaggtgt
    62941 tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag
    63001 tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt
    63061 gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc
    63121 tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat
    63181 tttgccagtg acttatctat ctctaggcgg atacatgctt ggattgatac taggagcttc
    63241 agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat
    63301 tatgccggtt cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt
    63361 ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa
    63421 tgtactcgtt tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat
    63481 gaggaatgaa cgaaaaaaag cataaaacaa tcctaaaata cagaaggaag ctggacatct
    63541 attgttccca gcttcttttt gtatagacct attttattta ttttactata ttggatatat
    63601 attcattgaa aatagtgtat aacaacttta taatattgag tataccaatt tttggaggcg
    63661 ttttaatgga ccgttattta gatttacttt cagaaaaatt cccctcaacg gaggatgtca
    63721 tcacggaaat catcaatctc gaagcaattt tgcagcttcc aaaaggaaca gagttatttt
    63781 tgagtgatat acatggcgaa tttcctgctt ttgatcatat tctgcgtatc ggttcaggaa
    63841 atcttaaaga aaagatcaaa gacctttttt ctgaacaaat gacggaaacg gatatcagcc
    63901 aattcactat ttttacggct tatccagaat atgcattaac gactgattgg tacaaagaac
    63961 aagataaatc gcagctgatc catcatttga tcgatctttt acggtttacg acagtcaaat
    64021 atacccggtc aaaagttcga aaaggattgc cgaaagaata tagctatatc attgaggaac
    64081 tcttgtatat cgatgaccgt atcaatggaa aaaaagacta tgtaaaaaaa atcattgaac
    64141 aacttgtttt gatgaaggaa gaagaaaaat ttttaacgaa acttgcacag acgatccaaa
    64201 aatctgtcat cgatcatttg catatcgttg gagatatttt tgaccgagga actcaagcag
    64261 ccaaagtgat ggatcgaatc atggattttt cttctgtaga tatccaatgg ggaaatcatg
    64321 atattttgtg gattggtgcg tattgcggct ctgaggcatg tttactgaca cttttacgga
    64381 ttgcagcacg ctataattat ttatttgaat tagagaaaga atatgggttg aatttacgtc
    64441 cattgttttc ttttgctcat caaacttatc agaagaatcc agtatttgcg cctaaaaaca
    64501 gtaaaaatct atccgaaaga gagcaagaag aattagaaaa aatccatcag gcactttcta
    64561 tccttcaatt caaatcagaa catcagttat tggatcgtcg acctgaattt aaaatggacg
    64621 atcgtaaatt gctggaaaaa atcgactatg aagcatcaac gattgattta gaaggtcagc
    64681 tctatgagtt gaaaggaact tgttttcaaa cgatccagcc agatgatcca aaaaaattct
    64741 tggaagagga acaaaaagtg attgatagtt tgatgtattc attccagcac tctttgagaa
    64801 tgcaaaaaca tatgacgttt ttgatcgaaa aagggggaat gtatctcacg aacaatcagc
    64861 atgtgctgtt tcatggctgt atacctgtag acgagaaggg acagctctta gcatttgaat
    64921 tagaccaatc ttatcgcgga aaagaactgc tgggcttttt tgaaaaacag ataactgaaa
    64981 gcgcaaaaga tctgactgca aaagaagatt tagcaaccga tttgatttgg tatgcgtgga
    65041 gtgggccatt ttcgccttta ttcggcaaga gtaaaatggc gacttttgaa cgttattttc
    65101 tcacagagcc tcatacgcac gtcgagaaaa gcaatcctta ttatcacttg cgagatgaag
    65161 agtggttcag tgaaaaaatt ttagcagaat tcgacacaaa agaagaaggg tcagcaatca
    65221 ttaatgggca cacgccagta aaagtaaaaa aaggggagtc accgatcaaa gcaaatggaa
    65281 aagtatttgt cattgacgga ggtttatcca aagcttatca aaaaacgaca ggaattgccg
    65341 gttattcact attatgcaat tcttatggtt tccaaatcgt gacacattat ccgtttttgc
    65401 ctattgaaca acaatttgca aaaaaatccg accaaacaaa tgtgaaaaaa gtaatcgaac
    65461 agcaactacc acgcaaactc aatcgtgata caacaaaagg tatggaactg cagcagcaga
    65521 ttacccagct caaaaggctg ttggactatc gaaaggacga ttgagaagaa ttccagtgaa
    65581 cattgttcca tagactggag actgagacaa aagtcgtgtc gcagtctctt tttccggata
    65641 aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtgaaaag
    65701 ccattttcac aaaatttttc ttaatactcg aaactaaaca gctttttcac aaccccctca
    65761 gaaagtctat taaaattcat tcacaaagaa atcgaataag cgctgttcag atggatgtac
    65821 acggtgggta agttccggat gccattgaac accgaggatt ttatgcatat catctttgct
    65881 ttgaaccgct tcaatgagtc catcgttaga aaaagcgatg gcttttaata aaggtgaaag
    65941 atctttgact gcctgatgat ggtaagaatt cacaaaatat ggatcgggga gaaggttgct
    66001 taacaaactg tctgaaacaa tcttgacttc gtgagtcgta aattgcgggg cagtgggctg
    66061 ttgttcatgt tttactgacc actttggata taatgaaaga tcttgataaa gtgtaccttc
    66121 caaaacgaca ttcaatagtt ggagtccccg gcagactgca aaaattggtt ttctttgttt
    66181 caatgcttcc aagatcagtg cctgttcaaa ttggtctcgt tgaatatttg tttccgttag
    66241 ctttggatga ggatcttcca tgtaaagttg tggactgaca tcttgaccac ctgcaagaat
    66301 aagtttgtcg atttgtccaa tcagttgagg agctgaatct ggagacatga ctggcagaat
    66361 aagaggcaat ccttgagcat ctaaaactgc atccacgaat ccttgcggtg tatagctcac
    66421 ttgatttcct tggaaagtat ctgttgctcg aatcagttga tttccggcaa tgccgatgat
    66481 tggtttcatt tgtattcccc ctaaagtaga cttattgtaa caaggattta aaaaaaatta
    66541 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    66601 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    66661 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    66721 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    66781 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    66841 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
    66901 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
    66961 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
    67021 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    67081 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
    67141 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    67201 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
    67261 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
    67321 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
    67381 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    67441 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
    67501 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
    67561 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    67621 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    67681 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    67741 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
    67801 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
    67861 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
    67921 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    67981 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
    68041 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
    68101 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    68161 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    68221 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
    68281 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
    68341 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
    68401 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
    68461 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    68521 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
    68581 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
    68641 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
    68701 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
    68761 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
    68821 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
    68881 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
    68941 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    69001 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
    69061 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
    69121 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
    69181 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
    69241 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
    69301 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggtacgca
    69361 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
    69421 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
    69481 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
    69541 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
    69601 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
    69661 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
    69721 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
    69781 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
    69841 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    69901 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
    69961 caaatccagt ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc
    70021 attgctggag aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat
    70081 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    70141 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    70201 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    70261 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    70321 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    70381 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    70441 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    70501 ggatctatac gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga
    70561 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    70621 aggatgccat tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac
    70681 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    70741 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    70801 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    70861 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    70921 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    70981 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa
    71041 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    71101 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    71161 ttcgtttact ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg
    71221 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    71281 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    71341 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    71401 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    71461 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    71521 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    71581 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    71641 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    71701 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    71761 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    71821 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    71881 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    71941 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    72001 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    72061 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    72121 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    72181 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    72241 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    72301 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    72361 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    72421 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    72481 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    72541 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    72601 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    72661 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    72721 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    72781 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    72841 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    72901 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    72961 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    73021 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    73081 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    73141 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    73201 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    73261 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    73321 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    73381 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    73441 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    73501 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    73561 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    73621 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    73681 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    73741 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    73801 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    73861 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    73921 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    73981 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    74041 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    74101 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    74161 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    74221 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    74281 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    74341 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    74401 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    74461 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    74521 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    74581 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    74641 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    74701 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    74761 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    74821 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    74881 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    74941 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    75001 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    75061 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    75121 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    75181 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    75241 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    75301 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    75361 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    75421 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    75481 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    75541 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    75601 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    75661 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    75721 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    75781 tacatcctaa aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta
    75841 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    75901 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    75961 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    76021 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    76081 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    76141 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    76201 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    76261 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    76321 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    76381 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    76441 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    76501 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    76561 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    76621 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    76681 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    76741 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    76801 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    76861 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    76921 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    76981 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    77041 gtacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    77101 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    77161 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    77221 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    77281 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    77341 tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    77401 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    77461 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    77521 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    77581 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    77641 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    77701 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    77761 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    77821 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    77881 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    77941 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    78001 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    78061 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    78121 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    78181 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    78241 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    78301 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    78361 aattttacca ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct
    78421 atgaaaatta ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa
    78481 gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg
    78541 gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg
    78601 ggggagaaca gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat
    78661 catttacctt ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa
    78721 gcattgataa acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca
    78781 acagattact tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa
    78841 gcattgtcgc atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac
    78901 gatattttag aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa
    78961 ataacgttta taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt
    79021 acgttgacct ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat
    79081 atcaatgctg tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa
    79141 acttctcaaa tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt
    79201 atagccatgg cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta
    79261 gtttctgaga aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt
    79321 ggtctagcta ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg
    79381 gctttaaaat gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag
    79441 gaaatccagc agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa
    79501 ttgccattga aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg
    79561 agcttagatg gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa
    79621 cagcatttgt tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc
    79681 aaagcggtaa tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta
    79741 ttatgccgaa aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta
    79801 aagaagccta cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta
    79861 aattgcagcc agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga
    79921 cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca
    79981 atgaagatcc agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa
    80041 tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg
    80101 aattaaaaga agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct
    80161 cgtttatttg atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat
    80221 agctacaatg gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc
    80281 tcattttaat tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac
    80341 tatcctcgga tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc
    80401 gcagtgccag gtaatccttt taagtcagta atctcaagac cagcagcttt atgttgctcg
    80461 tgtaattttt ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca
    80521 tacgcaccat gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc
    80581 aaataaacaa gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca
    80641 aatgcctttt gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga
    80701 gccattttca tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa
    80761 ggaaatagtt gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata
    80821 aaactgttaa ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt
    80881 gctgattatc aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca
    80941 gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag
    81001 acgaaagaag aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt
    81061 tcttcttatc tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat
    81121 tataatccag gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa
    81181 aagaccaata aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt
    81241 agccaccgat gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt
    81301 agatcagctt tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc
    81361 tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac
    81421 ggcatttccc attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt
    81481 atctccgata gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac
    81541 tgccgcacct ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac
    81601 cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc
    81661 tggttcgtcg atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt
    81721 tcgatatttt agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc
    81781 tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg
    81841 tgccatcact tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt
    81901 acctgatttc gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg
    81961 aaaaaggttc aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa
    82021 aacgatcgaa acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac
    82081 ttctcttttc tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat
    82141 aatttcctcc aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc
    82201 aaattcttga taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat
    82261 ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag
    82321 ctgactaacc tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt
    82381 gcgatgtctg ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac
    82441 agcagtgtaa taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac
    82501 taattttgaa tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa
    82561 acgtttagct aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt
    82621 gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa
    82681 tggcagaagt aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg
    82741 tgcgcgatga agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta
    82801 cacttgcaca tacatttgat atcggctaac gatataatct tccactgcat gcatgccact
    82861 catggaaaaa gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc
    82921 aaatgttcca tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc
    82981 tgcatcgatc tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat
    83041 cacactagct accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc
    83101 tgtttctgga gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatatg
    83161 ttcaaatgta tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt
    83221 caccagccgt tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg
    83281 agaaaagata tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg
    83341 gctatgttct gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag
    83401 acgttggact tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata
    83461 gttatggact ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt
    83521 tgtcataatg tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc
    83581 attgctccag ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag
    83641 aatacagctt ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt
    83701 ggtcaagcag ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg
    83761 taccaaagtc ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccgcgttttt
    83821 cctgattacc gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct
    83881 gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag
    83941 aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga
    84001 gcatttgctc gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca
    84061 aagaaatatt caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca
    84121 ctggagcgta acctgcatct ttcagtgttt ggatcccgtc acttagaaaa ggagttcgaa
    84181 catccttcat acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct
    84241 ggtgttcttt agaatattcc gttaatatat ctgttaatgc aaaaggggaa aagtcgtttt
    84301 tttgataaat gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata
    84361 aattcatatt ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt
    84421 ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat
    84481 gagaagtaaa ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt
    84541 gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca
    84601 aatggtgaca attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg
    84661 cgatgagatt cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc
    84721 aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg
    84781 aacaccatac ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa
    84841 agaccgtccc acagcaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa
    84901 aatcggcgaa tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat
    84961 tttttgtgtc tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga
    85021 aattagtgta tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc
    85081 gatcttagaa caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa
    85141 ctatcttggg aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt
    85201 gaatattgac ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta
    85261 tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac
    85321 tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat
    85381 cgattacaat gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct
    85441 gtttgatgac gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga
    85501 aatcggagca gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga
    85561 tttaagtatc atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga
    85621 ctctgaaaaa gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta
    85681 tcagaataag gaggtgtgac acgatttttg tcacaccttc ttttttgtat ataaaaatcc
    85741 caagtaaata cataaaaaat aaaaaaattg aaacttttag cgcaccttgt acgtcatatt
    85801 aagtgaggca gaaaggagtg gagaatgatt tttactggat ataagaaaaa acggcagatc
    85861 gaaaaatacc gtcacctgct gaatggactc atcgaaactg attatcaaaa aatgttccgt
    85921 atcacgatga attacgtaca tgataaagaa gaggccttag atgtgatgca agatagtttt
    85981 cataaagccc tgaactattt tgaaaaagaa aaagagatcg agcattttag tgcatggttt
    86041 tatcggatat tgatccgcac ggcattagat agttggcgca gacaaaaaag gacgcctgtg
    86101 gacttatttg aaattcaaga tatcccccaa cagttacctt tagaaacttc cgttgcagag
    86161 ctccatatga ttttagataa aatagacagt ccggaaaaag agattctgat cctctacttt
    86221 tttgaaggat ttcgtttgaa agaaattgcg actatcttgg atatgaatga aaacacagtt
    86281 aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa tcctagaatg aaggagagaa
    86341 gagtgccatg tattcccaaa aagaaaattt aaaaaaacta aagaaaactt ataaagaaca
    86401 agcaattccc aaagggctta aagaggagat ccgccagcgt tttattatcg aagagcaatc
    86461 atttataaaa gcaaagcgga gaaagagacg tctccaaggg ttcggagtat ctttggctat
    86521 cgtaatgatt acaacaagta gccttctttt taatagtcaa gttcgaagct ttgcagaaga
    86581 tttacccatc ttaggatcag tcattgaatt gatcttaggc gaacgcttta ctgatcgatc
    86641 tgaaaaaatc gatattcagg taccgaagat caatacacaa aatgagaaag aaaataaaac
    86701 aattcatgga ttaaatcaaa aatactttcg cgaaggacaa gcagattttg aaaaagctga
    86761 aaaagaatat ggaaattttg aaacggatca ttaccaagtt ttgggagatt atcagaaaat
    86821 cgtagacgat aaccggtttc tcgtgattga aaggcagatc acccaaacag cagcagatag
    86881 ccatgttgaa aaaagatacg acacaatcga caagaaaaac agtgtccagc tctcacttcc
    86941 acttttattc aaagatgaca cctatctatc tgttttgaca aaagaagtca aacggcagat
    87001 ggccgaacaa gtcaaggaag atccatctaa gtactattgg actgagcaag atattcaaga
    87061 agggacgata gaaaaaccaa cacttgttac gcctacacga agtttttatt tgaacaaaga
    87121 gcatcaatta gttttgactt tttctcaata cgaaattgct cctggttata tgggtacacc
    87181 agaatttgtc attcctaagt ccgtgacaaa aacgattttg gcatcggaag attatttgga
    87241 tcattagacg agaagtagtt gcgactagca gtttcaattt tcaagtaagt acaagtaaat
    87301 aataagaaga gaaaaagaga aagaagagat atagtgaaaa gaaattattt agttatcagt
    87361 attattgctt tatccgtgct tttgtttttc gaaattactt cggtggcctc cttgatcacc
    87421 gaagtaaaag gacaaacaca ctcttcggag cattcgtcac aatctttcaa acaatcagaa
    87481 caatcaacca ttaaaagcca atcaactgaa acagctaacg tcaaagaagc cattcaagaa
    87541 tcagcggaaa aggtacaaag tcagtcatca caatcaacac aagacagtac aaaaacgagc
    87601 cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg atggaccagg agcgaccaca
    87661 acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc atgccacttt tttcgtctta
    87721 ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga cagctgaagc agggcacgag
    87781 attggcaatc acacatacga tcatcagaat ctggccactc tttctgccca gtcgatgacg
    87841 gaggaagtga cgaaagcaga cactgagatc aaaaaagtca ctggcaaaac ccccactttt
    87901 gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa cgatcatcca gcgaccgatc
    87961 atcgaatggt ctgttgattc agaagattgg aagacaagaa atcctgattt gattttgcag
    88021 aagatccaag caacagtata cgatggggca attattttat tccatgatat ctatcctgaa
    88081 acgattcgtg ccgttcctca agtgatcgat tatctgaaag agcaagggta tcgcatcacg
    88141 acagttgggg atttattagg gcatccaacg gctgtcgaga actattatgg cagaaacgat
    88201 catcggccag ttcagtaagt gagacaagta tctttaataa ataacacatt ttaataagta
    88261 gttgaacaca taactacctt acttgcaaag gcttaacaat atctatacag gtacaacaaa
    88321 aaactgtcca ccaattttct ttggcggaca gttttcactt aaaaataaag aagcaactat
    88381 tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca
    88441 agaaccggaa tcttacgtga tatccactac actaaggacg caagcactgg gataattgta
    88501 actaaacaga taaaaaatta caaggggatt ggacaaaaaa atgaaatttg agcaaaattt
    88561 ttcacaaaaa cagcagcaga cacagaaact agccatgacg caacagttgc aacagtccat
    88621 ccaagtttta caatataaca gtgaggagct gttggcattc gttgagaatc aagcaatgga
    88681 aaatccattg gtggaagttg ttgagccgga atggcagcca gattacataa aagcttcttc
    88741 atcttcttat gaaggagaag agacaaatta cctaaatcag attcctgata caaaagggtc
    88801 tttatttgat tcattgatcg aacaagtcca tttgaattac cgagatactt ttttgaggaa
    88861 aatcgttctt tatcttgtag aatacatcga tttgaacgga tttttgacga ttagtttaga
    88921 agaagccgga aaagagattg gagctacgcc tatccaaatg ctcgatgcgt tgactctgat
    88981 tcagcaatta gaaccagctg gagtaggtgc acgtagtttg caagaatgtc tcatgcttca
    89041 aacagaacga gatgattatg cgccggaatt agcttatatc gtgttggaag aatgttttga
    89101 ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt ttttctgttg atctacatgc
    89161 tgttcagcag atattcgact atttacaaac gttgagtcca agccctggcc ggattttcga
    89221 ccgtagcagc gaattgtaca ttattcctga tgtccgggta ttagtggacg acgataaaaa
    89281 tgtacaagtt atctcaaacc gaaaaaatca gccaaatata cgctttcaag agagttactt
    89341 caaacaaatg tcacaacaag cagataaaga aactgaaaat tatttaaaag aacggaaaca
    89401 ggaattcgaa tggttgaaga aaacgatcct acaacgtggg gatacaattt tgcgggttgc
    89461 tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc
    89521 tcttacgtta aaggaagttg caactgagat agatgtgcat gaatccacgg tcagtcgtgc
    89581 agtcaacggg aaatatttgg aaacagactt cggtgtattt gaattaaaaa aatttttcac
    89641 tacacgaatt cctacgaata gtacagaaca gacagaagac ctttcagcag atacagcgaa
    89701 gaaaaagctg caggaattag tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa
    89761 attggttgaa ttgctgaaaa aagatgagat agcgatctca agacgaacag tagcaaaata
    89821 tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa cgttatgata actaatcagt
    89881 caaaagacta gaaaaagtat ttcacttttt ctagtctttt tagttgaaaa acgagagcaa
    89941 aactgttaga cttacagtcg tgaggttgag atttcccttt attgatgtga aattcgaata
    90001 gttaatttct ttttttatcg tatatgggtc gttttaagtc tacattggac gtaaagtgtc
    90061 caactaagga gatcgctatg ctggatgaaa taaaaatgat tgaatctgtt gcaccagata
    90121 ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa tatctattgg atgcagccga
    90181 ttggacgtcg aagtttatcg gaaagtatgg ggatcacaga acgagtattg cgaacagaaa
    90241 cagatctttt gaaaaatctg aatttgatcg acacctcaaa aagcggtatg acgctgacaa
    90301 ctaagggcga agaagtttat caaagccttg aaaacttcat ggatcagctt ttaggtacgc
    90361 atcaaaccga acaacagttg gcagaatatt ttggtaacca acgctgtatc gttgtttcag
    90421 gaaacagcga agagcagacc aaagtggctg atgcattcgg cgaagcgctg agcgaagccc
    90481 tagaccgact acttccagaa ggcgagaata tcatcgctgt gatgggagga actactatgg
    90541 ctgtggtagc cgaacaattg tctaatctgg aaaataagaa acggcataac ctgtttgtcc
    90601 ctgcaagagg cgggattggt gaagcgataa ctgtacaggc aaattctgtc agtgcaagaa
    90661 tggctgctaa agcaaacggt aaccaccgtg cactttatgt tccggaacaa ttaagtctgg
    90721 caacttacaa ttctttactc aatgagccat ctatccaaga agtcttaaac ttgattagtg
    90781 aagcgaattg tgttattcat agtattgggc gtgcattaca catggcagca cgtcgtaaga
    90841 tgactgaaaa agaattagtc atgctgaaac aagcaaatgc tgttgcggaa tcttttggct
    90901 actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc tagaatcggc atagaattgc
    90961 aagatttgga aaaagtcccg atcatcatgg caatcgcggg cggaaaatca aaagctaaag
    91021 caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg gctcatcact gatgaagctg
    91081 ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa atattttttt gattttcata
    91141 aggaggaaat cttaaatgac agttaaagta ggtattaatg gttttggacg tattggacgt
    91201 ctagcattcc gtcgtatcca agatgtagat ggaatcgaag tagtagcaat caacgacttg
    91261 acagatgcta aaatgttggc acacttgtta aaatacgaca caactcaagg acgtttcaac
    91321 ggaacagttg aagttcatga aggttcattc aacgtaaacg gaaaagaagt taaagtatta
    91381 gctaaccgca acccagaaga attaccatgg ggcgaactag gcgtagatat cgttctagaa
    91441 tgtactggtt tcttcacttc taaatcagca gctgaaaaac atttaacagc tggtgctaaa
    91501 cgcgttgtta tctctgctcc tggcggaaac gatgttccaa caatcgttta taacacaaac
    91561 cacgaaacat taactggtaa agaaacagtt atctcaggtg cttcatgtac tactaactgt
    91621 ttagctccta tggctaaaac attgaacgac aaatttggtg ttgttgaagg attaatgaca
    91681 actatccacg cttacacagg tgaccaaatg actctagacg gacctcatcc taaaggtgac
    91741 ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta actcaacagg tgctgctaaa
    91801 gctatcggtt tagtaattcc tgaattgaac ggtaaattag acggagctgc tcaacgtgtt
    91861 cctgtaccaa caggttcatt aacagaatta gtaacagttc ttgaaaaaga agtaactgtt
    91921 gacgaaatca atgcagcaat gaaagaagct tcaaacgaat cttacggata caacacagac
    91981 gaaatcgttt cttcagatat cgttggtatg acttacggtt cattatttga tgctacacaa
    92041 actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa ctgttgcttg gtacgacaac
    92101 gaaatgtcat acactgcaca attagtacgt actttagaat acttcgctaa cttataagat
    92161 agacaaacgt acaagtgtaa attatagaca tgtacattta aaagcgggga agcaatcgcg
    92221 cttcttccgc tttttttgat aataagttta ttcaggagga aataaaatgg ctaagaaaac
    92281 agtgaaagat atcgatttaa aagacaaaaa agttcttgtc cgtgttgact tcaacgttcc
    92341 tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa gcagcattgc caacaatcaa
    92401 ctacgttcta gaacaaggcg gaaaagcaat ccttttctct caccttggac gtgtaaaaac
    92461 agaagaagat aaagaaggaa aatctttagc tccagttgca aaacgtctag gcgaattatt
    92521 aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa caattagaag aagcaatccg
    92581 caacatgaat gacggcgacg ttgtcgtatt cgaaaataca cgatttgaag atattgacgg
    92641 taaaaaagaa agcaaaaatg atacagaact aggtaaatac tgggcttcat taggcgatgt
    92701 atttgttaac gatgctttcg gtacagctca ccgtgcacat gcttctaacg taggtatcgc
    92761 ttcaactggt atcccaacag ttgctggatt cttgatggaa aaagaaatca agttcatcgg
    92821 tgaagcagta gaagaaccaa aacgaccaat gattgcaatc cttggtggcg caaaagtttc
    92881 tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca gataaaatct tgatcggtgg
    92941 cggaatgact tacacattct acgaagcaaa aggaatcaag atcggtaact ctctagtaga
    93001 agctgataaa gtggaactag caaaagaatt aatcgaaaaa gcaggcgaca aacttgtctt
    93061 gccaatcgac aatgtctgtg caccagaatt ttcaaatgat gttgaaacac aagtaatcga
    93121 aggagacatt cctgatggtt tgatggcttt agacatcggc ccagcatcag taaaattatt
    93181 tgctgataca ttacaaggtg caaaaacagt tgtttggaac ggacctatgg gtgtatttga
    93241 aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa gcaatcgcta acttagaagg
    93301 cgcaacaaca atcatcggtg gcggagactc agccgcagca gcagaacaat taggatttgc
    93361 tgacaaattc acacacatct caacaggtgg cggcgcaagc ttggaattgc ttgaaggtaa
    93421 agaattacca ggactagcag caatcaacga taaataaacg accaagtttg ctcaattctg
    93481 acgagtaatc gaacaaaagg tgaagggagt tttcatgact ccctagccat ttttcaataa
    93541 attaaggagt gtgttcctca atgcgtaaac ctattatcgc aggtaactgg aaaatgaaca
    93601 aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa aaacaaaatc ccttcaaatg
    93661 acaaagttga ttcagtcatc ggatctcctg cattattctt acaagaatta atgacatcag
    93721 ctgaaggtac agaactaaaa atctcagcac aaaactgcta ctgggaaaac tcaggtgcgt
    93781 tcactggtga aacttcacca gcagctcttg ctgatctagg tgttcattat gtgatcatcg
    93841 gacattcaga acgtcgtgaa tatttccatg aaacagacga agacatcaat aaaaaagcga
    93901 aagctatctt tgcaaatggt atgacaccaa tcctatgctg tggcgaatct ttagaaacct
    93961 atgaagcagg caaaactgct gaatggatcg aaggacaaat tacagctggt ttagctgatc
    94021 tttcagctga acaggtgtca aacatggtta ttgcttatga accaatctgg gcaatcggta
    94081 ctggtaaatc agctgacgca caaatcgctg acgaaatttg tggagttgta cgtcaaacag
    94141 ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg tatccaatac ggcggttctg
    94201 taaaaccaga aaatattgct gaatacatgg caaaagaaaa tgttgacggt gctttagtcg
    94261 gtggcgcaag cttgcaagca gattcattct tgtcattgtt agacgctgta aaataattgt
    94321 tgaaaatttg cgaaaaatgc aagaaatgtt tgcactaatg agcaaaatta actaaaatcg
    94381 tatatgaggg aaatccctta ggaaaaaaca aactcaaagg agagacaaaa catgtcaatt
    94441 attactgatg tttacgctcg cgagatctta gactcacgtg gtaacccaac aatcgaagta
    94501 gaagtataca cagaaagcgg agcttttggc cgcggaatgg ttccttcagg tgcttcaact
    94561 ggtgaatacg aagcagttga attacgtgac ggagacaaag ctcgttatgg cggtaaaggt
    94621 gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg aagcaatcat tggctacgat
    94681 gttcgtgacc aaatggctat cgacaaagct atgatcgctt tagacggaac tcctaacaaa
    94741 ggtaaattag gtgcgaacgc tattttaggt gtttctatcg ctgttgcacg tgcagctgct
    94801 gactacctag aagtaccttt ataccactac ctaggcggat tcaacacaaa agtattgcca
    94861 acaccaatga tgaacatcat caacggcgga tcacatgctg ataactcaat cgacttccaa
    94921 gaattcatga tcatgcctgt tggcgctcca acattcaaag aagctctacg tatgggtgct
    94981 gaagtattcc acgcattagc ttcaatcttg aaagctcgcg gattagcaac ttctgttggt
    95041 gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag aaggttttga agtaatcatc
    95101 gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg ttgttcttgc tatggatgct
    95161 gcttcttcag aattctacga caaagaaaaa ggtgtttacg tactagctga ttcaggcgaa
    95221 ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag aattagtttc taaatatcca
    95281 atcatctcaa tcgaagacgg attagacgaa aacgactggg acggattcaa gaaattaact
    95341 gacgtattag gcgacaaagt tcaattagtt ggtgacgact tgttcgtaac aaacactcaa
    95401 aaattatctg aaggtatcga aaaaggaatt gctaactcaa tccttatcaa agtgaaccaa
    95461 atcggtacat taacagaaac tttcgaagct atcgaaatgg ctaaagaagc tggctacaca
    95521 gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa caatctctga tatcgctgtt
    95581 gcaacaaacg ctggccaaat caaaactggt tctctatcac gtactgaccg tattgctaaa
    95641 tacaaccaat tactacgtat cgaagaccaa cttggtgaag ttgcagaata caaaggtttg
    95701 aaatctttct acaacttaaa aaacaaataa ttcttttcta atgaagatac atgatcgact
    95761 attgaaagat agtcgagtat gatcacttag ttaagtaatc gaactctcta cttgctgaaa
    95821 tatcagcagg tagagagttt ttttgcaact agcttctgta tatgatgttt acaaagaatg
    95881 gtacaagaaa agatcatcct tgaaaaagga aagctaacaa taactagcat catataagag
    95941 cttttaagga tagaacagta gaataggaat ctatgagcaa gtcctctcgt ataaataaaa
    96001 taggagggaa gtagcagcat atttttcata aagtggctct ctttcttcat ttttactttg
    96061 gttgctccta tttgaaaatt cgagcacact caaatacaat aaaaagaaat ggcaagggag
    96121 aggattagat gaaaaaagaa gaaaaaatcg cgattttaca agagatcatc cgaattaaat
    96181 cagtgaatgg aaacgaaggt gaagtagcag cctatttgaa taagcttttg gcaaagcatg
    96241 acataacagg agagattgtt tcttacagag atggtagaga taatttgatt gctcgttacc
    96301 aaaagggcca atcaggaaaa gtgttaggcc tatctgggca tatggatgtc gtggcggctg
    96361 gtgatgaatc gtcttggacc tatgcaccct ttgcagcaga aatccatggg aaccgtttgt
    96421 acggacgtgg tgctacagat atgaagagtg gcttggcagc aatggtgatt gccatgatcg
    96481 aactgaaaga gtcaggaaaa ccatttaacg gaacagtcaa attattagcc actgtcggag
    96541 aagaagtggg tgaactaggt ggggaacagc tcacgaaagc aggctatgta gatgacttag
    96601 atgccttgat catcggagag ccaaccaatt acagtttgat gtatacgcac atgggatcaa
    96661 tcaactatac agtcacctcg cacggaaaag aagcgcatag ctctatgcca gaccaaggtt
    96721 ataatgcaat caatcaccta aatgaattca ttacgaaagc aaatgctgaa atgaatcacc
    96781 tcgctgaaac aatcgagaat cctgtcctag gaaaaacaat ccataatgtt acattgatca
    96841 gtggcgggaa tcaagtcaac agcatcccaa gccatgcgca gctccaaggg aacattcgat
    96901 cgattccaga atacccaaat gataaaatca ttgcattgct gcaatcaatc gtcaatgaat
    96961 tgaatcaaga gacagactat catttggaat tgatgatcga ttacaacaaa atacctgtca
    97021 aagctgatcc agattctcct ttgattcatt gcattcagca acaattcagc cagccattac
    97081 ctttagttgg agcggcagca acaacggatg cagccgaatt tacgaaagct aaccattcct
    97141 tcgactttgt cgtctttggg cctggtgtcg ttactttgcc tcatcaagta gatgagtatg
    97201 ttgaaatcga taattatttg gatatgatcg aaaaatatca aggaattatt ttatcctact
    97261 tagcgtaaga tttttttaat aaatgaaaaa acagagccag attagcatta
//
LOCUS       DAZUJO010000002        96998 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000002 DAZUJO010000000
VERSION     DAZUJO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 96998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.002"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8OL_000100"
     CDS             complement(59..187)
                     /locus_tag="ACG8OL_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4816122.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8OL_000101"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8OL_000101"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4816123.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8OL_000102"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8OL_000102"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4816124.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8OL_000103"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8OL_000103"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS4816125.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8OL_000104"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8OL_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS4816126.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8OL_000105"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8OL_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS4816127.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8OL_000106"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8OL_000106"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS4816128.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8OL_000107"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8OL_000107"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS4816129.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8OL_000108"
     CDS             10491..12050
                     /locus_tag="ACG8OL_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS4816130.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8OL_000109"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8OL_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS4816131.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8OL_000110"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8OL_000110"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4816132.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8OL_000111"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8OL_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4816133.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8OL_000112"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8OL_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816134.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8OL_000113"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8OL_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816135.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8OL_000114"
     CDS             17616..17771
                     /locus_tag="ACG8OL_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS4816136.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8OL_000115"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8OL_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS4816137.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8OL_000116"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8OL_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS4816138.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8OL_000117"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8OL_000117"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS4816139.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8OL_000118"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8OL_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816140.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8OL_000119"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8OL_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4816141.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8OL_000120"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8OL_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4816142.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8OL_000121"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8OL_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816143.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8OL_000122"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8OL_000122"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS4816144.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8OL_000123"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8OL_000123"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS4816145.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACG8OL_000124"
     CDS             complement(28200..29369)
                     /locus_tag="ACG8OL_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS4816146.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8OL_000125"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8OL_000125"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS4816147.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8OL_000126"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG8OL_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS4816148.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="ACG8OL_000127"
     CDS             34320..34841
                     /locus_tag="ACG8OL_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS4816149.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="ACG8OL_000128"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="ACG8OL_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="ACG8OL_000129"
     CDS             complement(36284..36940)
                     /locus_tag="ACG8OL_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816150.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8OL_000130"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG8OL_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS4816151.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8OL_000131"
     CDS             complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG8OL_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS4816152.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8OL_000132"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG8OL_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS4816153.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="ACG8OL_000133"
     CDS             complement(40491..41222)
                     /locus_tag="ACG8OL_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS4816154.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="ACG8OL_000134"
     CDS             complement(41226..42491)
                     /locus_tag="ACG8OL_000134"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS4816155.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8OL_000135"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG8OL_000135"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS4816156.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8OL_000136"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG8OL_000136"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS4816157.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="ACG8OL_000137"
     CDS             47833..49335
                     /locus_tag="ACG8OL_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS4816158.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="ACG8OL_000138"
     CDS             49337..50074
                     /locus_tag="ACG8OL_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4816159.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50119..50292)
                     /locus_tag="ACG8OL_000139"
     CDS             complement(50119..50292)
                     /locus_tag="ACG8OL_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816160.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            50304..50933
                     /locus_tag="ACG8OL_000140"
     CDS             50304..50933
                     /locus_tag="ACG8OL_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816161.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(51015..52181)
                     /gene="dnaJ"
                     /locus_tag="ACG8OL_000141"
     CDS             complement(51015..52181)
                     /gene="dnaJ"
                     /locus_tag="ACG8OL_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS4816162.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(52332..54161)
                     /gene="dnaK"
                     /locus_tag="ACG8OL_000142"
     CDS             complement(52332..54161)
                     /gene="dnaK"
                     /locus_tag="ACG8OL_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS4816163.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(54212..54775)
                     /gene="grpE"
                     /locus_tag="ACG8OL_000143"
     CDS             complement(54212..54775)
                     /gene="grpE"
                     /locus_tag="ACG8OL_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS4816164.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54869..55912)
                     /gene="hrcA"
                     /locus_tag="ACG8OL_000144"
     CDS             complement(54869..55912)
                     /gene="hrcA"
                     /locus_tag="ACG8OL_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS4816165.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(56146..57312)
                     /gene="hemW"
                     /locus_tag="ACG8OL_000145"
     CDS             complement(56146..57312)
                     /gene="hemW"
                     /locus_tag="ACG8OL_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS4816166.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(57328..57555)
                     /locus_tag="ACG8OL_000146"
     CDS             complement(57328..57555)
                     /locus_tag="ACG8OL_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS4816167.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            58039..58646
                     /locus_tag="ACG8OL_000147"
                     /pseudo
     CDS             58039..58646
                     /locus_tag="ACG8OL_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            58819..59532
                     /locus_tag="ACG8OL_000148"
     CDS             58819..59532
                     /locus_tag="ACG8OL_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816168.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(59647..60657)
                     /locus_tag="ACG8OL_000149"
     CDS             complement(59647..60657)
                     /locus_tag="ACG8OL_000149"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS4816169.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(60694..61176)
                     /locus_tag="ACG8OL_000150"
     CDS             complement(60694..61176)
                     /locus_tag="ACG8OL_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS4816170.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            61326..61694
                     /locus_tag="ACG8OL_000151"
     CDS             61326..61694
                     /locus_tag="ACG8OL_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS4816171.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            62159..62611
                     /locus_tag="ACG8OL_000152"
     CDS             62159..62611
                     /locus_tag="ACG8OL_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816172.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(62735..63586)
                     /locus_tag="ACG8OL_000153"
     CDS             complement(62735..63586)
                     /locus_tag="ACG8OL_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4816173.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(63599..64384)
                     /locus_tag="ACG8OL_000154"
     CDS             complement(63599..64384)
                     /locus_tag="ACG8OL_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4816174.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(64736..65677)
                     /gene="ribF"
                     /locus_tag="ACG8OL_000155"
     CDS             complement(64736..65677)
                     /gene="ribF"
                     /locus_tag="ACG8OL_000155"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS4816175.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(65681..66604)
                     /gene="truB"
                     /locus_tag="ACG8OL_000156"
     CDS             complement(65681..66604)
                     /gene="truB"
                     /locus_tag="ACG8OL_000156"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS4816176.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(66972..69334)
                     /locus_tag="ACG8OL_000157"
                     /pseudo
     CDS             complement(66972..69334)
                     /locus_tag="ACG8OL_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            69499..69955
                     /locus_tag="ACG8OL_000158"
                     /pseudo
     CDS             69499..69955
                     /locus_tag="ACG8OL_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(70112..70459)
                     /gene="rbfA"
                     /locus_tag="ACG8OL_000159"
     CDS             complement(70112..70459)
                     /gene="rbfA"
                     /locus_tag="ACG8OL_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS4816177.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(70486..72792)
                     /gene="infB"
                     /locus_tag="ACG8OL_000160"
     CDS             complement(70486..72792)
                     /gene="infB"
                     /locus_tag="ACG8OL_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS4816178.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(72805..73116)
                     /locus_tag="ACG8OL_000161"
     CDS             complement(72805..73116)
                     /locus_tag="ACG8OL_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS4816179.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(73113..73406)
                     /locus_tag="ACG8OL_000162"
     CDS             complement(73113..73406)
                     /locus_tag="ACG8OL_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS4816180.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(73428..74603)
                     /gene="nusA"
                     /locus_tag="ACG8OL_000163"
     CDS             complement(73428..74603)
                     /gene="nusA"
                     /locus_tag="ACG8OL_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS4816181.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(74626..75099)
                     /gene="rimP"
                     /locus_tag="ACG8OL_000164"
     CDS             complement(74626..75099)
                     /gene="rimP"
                     /locus_tag="ACG8OL_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS4816182.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(75244..79602)
                     /locus_tag="ACG8OL_000165"
     CDS             complement(75244..79602)
                     /locus_tag="ACG8OL_000165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS4816183.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(79814..81523)
                     /locus_tag="ACG8OL_000166"
     CDS             complement(79814..81523)
                     /locus_tag="ACG8OL_000166"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS4816184.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(81591..82859)
                     /gene="rseP"
                     /locus_tag="ACG8OL_000167"
     CDS             complement(81591..82859)
                     /gene="rseP"
                     /locus_tag="ACG8OL_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS4816185.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(83020..83820)
                     /locus_tag="ACG8OL_000168"
     CDS             complement(83020..83820)
                     /locus_tag="ACG8OL_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS4816186.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(83817..84629)
                     /locus_tag="ACG8OL_000169"
     CDS             complement(83817..84629)
                     /locus_tag="ACG8OL_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS4816187.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(84824..85381)
                     /gene="frr"
                     /locus_tag="ACG8OL_000170"
     CDS             complement(84824..85381)
                     /gene="frr"
                     /locus_tag="ACG8OL_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS4816188.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(85384..86106)
                     /gene="pyrH"
                     /locus_tag="ACG8OL_000171"
     CDS             complement(85384..86106)
                     /gene="pyrH"
                     /locus_tag="ACG8OL_000171"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS4816189.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(86242..87123)
                     /gene="tsf"
                     /locus_tag="ACG8OL_000172"
     CDS             complement(86242..87123)
                     /gene="tsf"
                     /locus_tag="ACG8OL_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS4816190.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(87222..88004)
                     /gene="rpsB"
                     /locus_tag="ACG8OL_000173"
     CDS             complement(87222..88004)
                     /gene="rpsB"
                     /locus_tag="ACG8OL_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS4816191.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            88363..88842
                     /locus_tag="ACG8OL_000174"
     CDS             88363..88842
                     /locus_tag="ACG8OL_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4816192.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            89059..90150
                     /gene="phnW"
                     /locus_tag="ACG8OL_000175"
     CDS             89059..90150
                     /gene="phnW"
                     /locus_tag="ACG8OL_000175"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS4816193.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            90143..90271
                     /locus_tag="ACG8OL_000176"
     CDS             90143..90271
                     /locus_tag="ACG8OL_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS4816194.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            90275..90820
                     /locus_tag="ACG8OL_000177"
     CDS             90275..90820
                     /locus_tag="ACG8OL_000177"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS4816195.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(91274..92965)
                     /gene="argS"
                     /locus_tag="ACG8OL_000178"
     CDS             complement(91274..92965)
                     /gene="argS"
                     /locus_tag="ACG8OL_000178"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS4816196.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(93385..94332)
                     /gene="arcC"
                     /locus_tag="ACG8OL_000179"
     CDS             complement(93385..94332)
                     /gene="arcC"
                     /locus_tag="ACG8OL_000179"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS4816197.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(94447..95466)
                     /gene="argF"
                     /locus_tag="ACG8OL_000180"
     CDS             complement(94447..95466)
                     /gene="argF"
                     /locus_tag="ACG8OL_000180"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS4816198.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(95557..96786)
                     /gene="arcA"
                     /locus_tag="ACG8OL_000181"
     CDS             complement(95557..96786)
                     /gene="arcA"
                     /locus_tag="ACG8OL_000181"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS4816199.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        26646 a        20859 c        16990 g        32503 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagcc cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacga cgtcttgacg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga tttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgctacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gagcctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataatcagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatacatgtt atgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg tcatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt atccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac attcttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccaacac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattactgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttctg gtggacaaaa acagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacttct gcactcgatc ctgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atggggttcg ctaaagaagt ggccaatcgc gtcatgttca ttgacgacgg
    49981 aaacttttta gaagatggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc acggtcgcca
    50101 tgaagggacc gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc
    50161 ttgttttcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt
    50281 tttcttctcc atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag
    50341 aacggatcaa tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt
    50401 tgctcactgt acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat
    50461 tgctatcatt gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa
    50521 actttggaat tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg
    50581 ccaaacaagt ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg
    50641 aagatttaga cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg
    50701 taaaacgtat tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg
    50761 catgcttcag taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata
    50821 aaaacacctt gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa
    50881 actatgacga ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac
    50941 aaaaaaacca ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt
    51001 ttttactaga aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag
    51061 aaaccgtctt cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat
    51121 gcttctcttt gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg
    51181 tcgcctgttg caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta
    51241 cctgtctgtg ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg
    51301 acttcatccc ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa
    51361 ccttcacgat caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat
    51421 ggtccgccgt tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct
    51481 acaccagctg gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga
    51541 caggttggac aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga
    51601 cggctcatca ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga
    51661 catttatgac aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga
    51721 cagatttctt cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa
    51781 gacaagtcga ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac
    51841 cgaccgcctc ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca
    51901 gagaagccgc cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct
    51961 gtgcctgcat gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca
    52021 tatgcttctg atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga
    52081 tggtattttt tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt
    52141 gagagcccta agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt
    52201 tctcatcatt ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata
    52261 cttcaaacta tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt
    52321 cagtgaactg attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca
    52381 tctgctccgc cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg
    52441 tatagtttaa cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt
    52501 tcttcgatgt tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt
    52561 ttcacttctt cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat
    52621 agcaatgcat ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct
    52681 gcatttgctt ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa
    52741 gatttgatcg tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca
    52801 atcccgtttt tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct
    52861 ggaatatctg tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca
    52921 ccttgtaata catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt
    52981 gatttacttg ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt
    53041 gtttcgatac ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca
    53101 gtgatcacgc caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat
    53161 ttgtttggtt cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta
    53221 gatccaccga ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc
    53281 gcttgacgta ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt
    53341 gcacgtgtca atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc
    53401 aagctgattt gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct
    53461 ttcaagcgtt gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat
    53521 tctgctacca tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg
    53581 ccagctgttg acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa
    53641 gtaccgccac caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca
    53701 taggcaagag cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc
    53761 ttaccagcat cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata
    53821 actgcttttt cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg
    53881 atcattgctg agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct
    53941 gcttcaccga tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt
    54001 tttgcaactt cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta
    54061 cggtttcctt ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca
    54121 gagttagtcg tacctaagtc aataccgata attttactca taattaacgt ctccttcttt
    54181 aattgaaagt gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc
    54241 ttaacacgcg atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg
    54301 gtgtatcttc agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt
    54361 ctcctttagc agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca
    54421 agaccatttc cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta
    54481 atgcacgttc caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt
    54541 atttttgtaa ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac
    54601 gagcccgcaa gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga
    54661 attcttccgc ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg
    54721 gttctggctg agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac
    54781 agtcttcctt tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga
    54841 tcagctgcaa atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt
    54901 aatcagcaag tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag
    54961 aataaggcat actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct
    55021 cgtaacttgc ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa
    55081 tcttgatatg aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt
    55141 tcataaaaga aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga
    55201 gattcatttg tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa
    55261 ataactgaga catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg
    55321 tacgaagttt atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct
    55381 gtaccatttt ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt
    55441 ctacattccc catatctgtt acgacgatag caataatctg tcgattattc aagggtacga
    55501 tacgaaagcc agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc
    55561 ttgtcagctc agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt
    55621 ctctgcctag agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta
    55681 acaaatgatc cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt
    55741 gagtcttcaa cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg
    55801 agctggcctt gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg
    55861 tatagtgttg gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca
    55921 cctctcatta gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc
    55981 cttctcctgt caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc
    56041 taaataattg atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg
    56101 aacttttttt atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa
    56161 aaagcgctca aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc
    56221 ttcttcatta acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga
    56281 ttcaattgtt ttttgatagc gatcatagaa atgtttttta gtaatccctg ttttttttct
    56341 tagtcctaag aacatttctt cttctatttg attttttcgt gtcaattttt cttcttccaa
    56401 taccgggcgt tcatttgctc gtaatgggcg taaatagtgt tggattggtc ctttgttgcg
    56461 gtaacgaata tctccaagat aaccgctagc accagctcca aacccaaaat agtgggcatt
    56521 gttccagtac atgaggttat gttgagattc atgtccggag agcccaaagt tacttatttc
    56581 atattggtga cgtccatgct tttccattgc ttcaatcgtc tcttcaaaca ttctggtttc
    56641 cgtttcctga tcaggcagtt cgagtcttcc ttgtcgtacc cagttcatga acattgtttt
    56701 gttttcgagg atcaatgagt acaaagaata gtgaggcaga tccaaagcca aagctttttc
    56761 caaagtatcc agatatcctt ctagtgtttg tcccggtaat gcataaatca aatcgatgct
    56821 tacattggta aaattttctg cttctaaaaa ttgcatagtt tgatacacat cttctgcggt
    56881 gtgtttacgt ccaatttttt tcaataagcg attatcaaat gtctgcactc ccatggataa
    56941 acgattgacg ccatagtttt tcattacttg aagtttttca cgggtcaaat cacctggatt
    57001 cgcttctaca gtaaactcat taccattttg aaaaggcagg atctctctag cacctgataa
    57061 cagtcgatca agttgtttag cagataaaga agttggtgtc ccgcccccta cgtaaagtgt
    57121 ttctaaattc gctgtcggat gactttctag cattattttc atttcattca gcagcatttc
    57181 cacatactca tctactggct gcccttctaa aaaaacttta ttaaaatcac agtaatagca
    57241 gatatgctcg caaaatggga tatgaatgta cgcagaaacc ttgttttctg cgggatttat
    57301 tgtttctatc attgaaattc tttctcctta cgtcgaaaag tcgtgcctgt tttcgacctt
    57361 cttaaaatcg cgttcaagca ccaactcatt gaaataagcc gcatatttag aatcaaggaa
    57421 acctagctct aaaaatgctt gcttattgtc atcggagcta cacggtgctt ttacagcctc
    57481 tcgataaacg gtaatcgcaa agctgacctt cggcattaag tcaaaatctg caaaaatatg
    57541 aaaagctatt ttcacagatt cctccttaag tgctcagacc acaacgcttt gcttacaacc
    57601 tctcatatac gcatactaaa cgtatttggc gaacaaatcc actaaaaaat ccaagaatcg
    57661 acttcttgga tgattttttc tttcattaaa ctggttacct agttatatat ctctagggtt
    57721 gttttcatgt ctgagtgccc gatatgattt atttttcgtt tctataatta ctttttgcct
    57781 tccagtttct tttaatattg tactcaagca agtgggaatt ggttcttttg tcatccatgt
    57841 atgcaaaatg ttataatgct ggtaaacaaa aatgattacc tgtcattttt atttagttcg
    57901 tatttaatta agttgactat ctgtatactc tgtctaaaac ttaagaattg ttgttttcgc
    57961 tgtcgctacc caattttaat gaatgtctgt aggttaaaac tgttgtcctt gttatagata
    58021 gtaaaggaga gcttgaaaat gaaaactgcg attacgtttc tattagagga atatgccgat
    58081 tgggaaggtg cttatattac tagcacacta aaccaaaatg agaaatggca aaacaaaact
    58141 gcttctatcc aatcagaggt cacttccatt ggaggattaa aaacaaaaat cgattactta
    58201 atcgaacaaa tacctgaaaa agttgacctt tttattatga tcggtggcaa ttcatgggac
    58261 ataaaaaatc atcatttata tcaaataatc gataaattgt taaaaacaga cgtaccagta
    58321 gctgctattt gtggggcagt tgattattta gctcagagcg gattattgca aggttacaaa
    58381 cacaccggaa atgcgcaatt tccttggtta gaatcggaaa attatcagaa taaagaagat
    58441 tttatagaac aacaagctgt gaaagatcgt aatttgatta ctgcgaatgg aacagctcct
    58501 atagaattca cagacttgat tcttcaagca gtccattttg aaacagaaga aataagagaa
    58561 aaaacgattc gtctatacaa acttggattc tatgattact ataaaaagta cggagatcct
    58621 ttctcctaat cattctcttc aattaaatac tccttatcaa tagtgtgaca agtaatttta
    58681 ctgatgatta tttgtcacac tttttctgac atttatcaaa gaagatacac tctatttata
    58741 ttcatagaca aggttttacg gattcaaaaa ttcaggatta tgctatactg gtattaaaaa
    58801 aataatgagg tattttatat gaaaaagtta ggttttattt tattgttggt ctctttattc
    58861 cttgtaggat gtacgtctcc tagtaaagaa aatagtgatc catctgcgac cacagacaaa
    58921 agttcaatca gacaaaccag ttcagctgaa aaatcaagta ctcagaaatc agcttctact
    58981 gaaacgaata catcttcaac aagcagtaaa gtctcagagc ccaatacttc ttctacatct
    59041 agtagtacag ttgagacaaa tatttcttct tcaacaatag acacaacctc ctcacagtct
    59101 tctcagtcaa tagatccgtt agcaggatat tctgatctac aaattgagta cgcacgtgtt
    59161 tggctagcta ttagaggtaa tacttttaga gatacatcgg gtgatttcga attacatgtt
    59221 attcattatc ctgcaggaac acctatcgcc ccttatgatg cagggagtgc agtctatccg
    59281 aaagatgtgg ttatgctcac tgggaaatac agttaccagg gagttttagt gtattcaagt
    59341 aaccatgacg ggacaatcac tagttatcca gttccttcac attggcaaat gcctgcagat
    59401 caaacatctt atccagcgtt cattagagaa acaactcaag aaatcattga taatgcatct
    59461 gtagtagcta ttcctacagg aaatcccaat gacgtgaaac agttaatcga tgtgacagtt
    59521 atagataatt agactttcta atatgttata caaaaagaag ctgacagcat tttgtcagct
    59581 tctttcactt tcttttctat ttagatttat tggtctttga tagactccaa ggcaggaaaa
    59641 aattttttaa ataaacgttt gcaaaacagt cgcttttcta tcttctaccg ctacttctgc
    59701 ataggcacca ttaatcaatg tcagttgtgg cggtacatga ccacaatcaa tatcatacaa
    59761 gatcggtagt tttaattcct ttgacagatc ttcgtataca tccttcattt tataatctaa
    59821 caaatcctct gccgcacttc taccgaacat gatccccgag ctgccatcaa accagcctgc
    59881 cagtttcatc tgaacaagtg atcttcttaa atcaactgac gatagctcac agttttctag
    59941 aaaccacaag atcggctttc catcattata ctttctttta aaagattgga catctccata
    60001 aggagtaccg attaaatgcc tgatcgtatc aatacaacca cctaataatc gtcccttcat
    60061 ccttactgca tctccgctga ctgattgcca atttgttggt tcagtgagat ggaaaacaga
    60121 agatgatgaa tgctcatggg gccacacttt ttgatacttt tcagaagatt gctgtagaat
    60181 cgattctcct tctcttgttt tcagtacact ttcccacatt gctgtcgttt catccaagta
    60241 ctctcctctt aaatcaataa ggttagttcc atgtgcagtg gctatccctg tttttaatgt
    60301 gacagcaagt agcaacgtac taatatctga ataacctact atccattttg gttgtagagc
    60361 atcaaaatcg acaaaatcga gaatctcaat caataattct cctccccaag tcggaaagat
    60421 catttggata ttttcatctt gcatcatttg attgaattca tctgctcgtt ttcttgctgg
    60481 acctgatttt tccttatctt gcgtccaaag cgtctttcca gtaactactt ggaaagattt
    60541 ttcttccatt ctctttaaag ctgattcgag taacgaatgg tgttcctctg atacgcctga
    60601 ggatggtgca gtaaccccaa tcgttgcatt tctttctaat aatggataac ttatcatgtt
    60661 gcttcacctc caagtatgca ctattttact tggttaattg tttttttcgt ttgccggcat
    60721 caaaaatatc ggcatctcaa gtagctggat gattttgttt aagtcaaccg tttggatgac
    60781 catcgtttga tctggtctac ctggtgcaaa aacaagtttt tcatttgata agagctcttc
    60841 atcaataata atcggaatct gtttttcata accaaacgga gaaacagcgc cctgcttttg
    60901 tcccgtttgt tcttccatgc gttctgatga aacaatagaa agccttttac cgacgatctt
    60961 ccctaatgct ttgaaatccg tttgtttcgt agtaaaagtc agatatacgt agttattatc
    61021 ttttttatct tttaagaata agcttttggt ctctgtacct gtaaatcctt gttctttctt
    61081 tattaccaaa gcatcttcat ttgtataaat ggctcgatga tgatacaact caaaatatat
    61141 attttcgtgt aagaatttct ttgccaagtc aatcattttt atgtctcttt tctattttat
    61201 tatttgagac atcttatcat ataaatatag cggttacaac attacttgtg ttttgttcgt
    61261 tttttataca taactattgt ttttctggta aaataaatca aaccacttaa tggaggcgtt
    61321 ttagaatgga aaaatttcag ccgatcatca atcaaatcaa agaaaaagaa catcaacaaa
    61381 agatcaaaga cctttttgct aagataacag aaacgtatcc ccagctagac acaactgtaa
    61441 aatggaacca acctatgttt actgaccatg gtacctttat tattgcattt agttttgcta
    61501 aaaatcattt ttctatcgca ccggaaaaag cagcgattcg tgcccttgaa aaaaatatcc
    61561 aagaagcagg ctacgtatat acggataacg tcatcaaagt tccttggaaa agtgtaatca
    61621 actgggaatt gattgaacaa ttgatttcat ttaatattga agataaaaaa ggtcatgata
    61681 aattttggcg ataaagcttt atacgaataa accttttact ggcgaggaaa ctattcatca
    61741 ccttatacag gttttgttca atcctatcct ttttatcatc cctggttaaa cacttaattg
    61801 acaaagtatc tgctattttt ataaggagtc tttcttgtgc tgaaaaaaat cactgattga
    61861 atggctttga attttctact tagtaaaatt attttttcag caattttcct gcaaaacttt
    61921 tgaataagga aaaaatacga gaaaatatca gtgatctttc ataatactgc ccattatttt
    61981 caggtatatt taaatataat atcactttag gagagaacga atttctttag agaaataaaa
    62041 gttagaattt tctgacttga tttttacaat gaccttaatt aaaatcgttc tttctacatt
    62101 tcgtgattaa gcaaatatca actaagtcag tagaaccatt cacataggag ggtgaacatt
    62161 gaaagatttt tactcagtaa atgaattagc cgaacaatta ggtgttacta cacgttctat
    62221 cagaaattat cttcatgaag gaaaactaaa aggaacaaag gttggcgggc aatggaaatt
    62281 ttctgaacgt aatctttttg agttcctata cggagatcaa gcagaggaag cagcaaaaga
    62341 aatgcagcga ttcatgctaa atgcaccaat cacgatgcgt ttcaaccttc aatatcgtga
    62401 ttttacagca atcaatcaat tccgagaaca attagtccag taccataacg atgtttacgc
    62461 aaataaaaaa gaccgtcttt tacaatacga tctttataaa gacaatcatg cggagatact
    62521 aatcggtggt aacttcaatt atgtcgtaaa ctttagtcaa tggatcaatg aaaaattatt
    62581 gatccagaca gatatttccc ttgtatctta aacaattaaa aagcgctaga aattccagag
    62641 atgttggaaa ttctagcgct atttttttat aagtacttac tgtttggttc aaacaaatat
    62701 ttttttaata aatgaaattt taccatcttt atcctcaaat aaagaccgtg accatgcttc
    62761 ctactacgat cagcaataat ccgataatcg ttaatttttt ttcttttggt gtcttatttt
    62821 catgcaggat gtacataccg cctaatgtag agataactac gtttagctgt gtaatgatat
    62881 atgcagtagc aaccccattt tcatttgctg aaaaaatata agccaatgct gacacactaa
    62941 agatggctcc aacgatcgtt gctttccaac tcttgtcttc agaaaatgcg gttggtttac
    63001 ggaaaatcac ataaatcact gcccctaaaa agacaccgat catttgcgga aagaaaatgg
    63061 aaagtccgct tgcatcaact agtttaggca atgcactgta tacccaataa ccaatacttg
    63121 ttgatgctaa tagactaatt ccttttgtga taccgccttt ttttccagga tcatcactga
    63181 ttgccgttaa agcagaacca aatacaagaa gaatgattgc tgctgctcca atgattttcc
    63241 ccatacttcc agaccattct ccaaaagcga agacaccaat caacgaggtt ccgactaact
    63301 gtaatgcagt cgaaattggc atcgttttag aaacaccaat caagttgaaa gatgtatact
    63361 gtcctgtttg tccaataacc caaaatgcac cagaaagtaa actaatcaaa aatgtagtca
    63421 ctgaaatacc agctggagaa gcgaataatt gaacgagtaa gccgacaagc accgctccaa
    63481 taccagttcc taatatctca tttgttggtc tgccaccaat ctttttcaaa atcagtggct
    63541 ggatccccca gccaactgcc gggatcaatg caatcaataa acccattttt attcaccctt
    63601 atccttttcc accttggaaa gaaggatata aagtcatccc accatctgca aatagtgtaa
    63661 tacctgtaac ataactggat tcgtctgaag ccaaccatgc agctgtcgcc gctatttctt
    63721 ttggttcacc tacgcgtcct agtgggatca tactttccaa gagttctttt tgttcaggat
    63781 catcaaattt tgccgcatta ataggtgtac ggatagcacc agggcctata ctattgattc
    63841 tgattccctt cggtgcatac tccattgcta ccgtttcggt aaataatttt accccgcctt
    63901 ttgaagcagc gtaatgagca aacgttggcc atgggatctg ttcatgaaca gaagacatat
    63961 tgatgatatt tcctttttta ttattctctg taaagtaatt taaagccgct ttcgttccga
    64021 taaaaacgcc agtcaaattg acttttaaca ctctttccca atcttcaagg ctaagttcat
    64081 gggttggatg ctgattttcc atcccagcat tattaaccca aacgtccagt gtcccaaaat
    64141 tttctaaagc cgtatcaagt aaacgctgta ttccttcttc agaactgaca tctgcttgta
    64201 cagagactgc ttttccgcct gcctcttcga ttgctttgac tgctttttct gctccttctt
    64261 tatccgagtt atagttgaca actacattca ttttttcttt cccgaatcgt tgagcaatcg
    64321 ctgttccaat cccctttgag ccaccagtta ctactgcgac tttgttttct aattccttgt
    64381 acatagtacc cactcctttt tcatacctaa acctgttatt acggataact tttatttccg
    64441 ccttaaagtg agtgtacacc aagatatata gtctaaagca aatgatacgc tctagcaatc
    64501 gttttcatga attgctagtg tccttttatt tctataaaga aagaggttgt gacagaagtg
    64561 gtcagcttca agaaatttcg gcttatttcc gaagaagcta cttctgaaaa accatttatt
    64621 tggaaaaaaa ggctgtgaca agacttttgt cacagcccct ttcctgggct tatttatcac
    64681 ccattttatt gtaattgcta gttatacata aacaagagat tttatgcata atgttttatt
    64741 gctgagaaaa atattcagct gttgcttgtt catcctcctt gagttgttca atcaattcct
    64801 ctacactaga aaatttcact tgatctctta aaaattgatt ccagcgtacc gcgacatgtt
    64861 caccatagat atcttgatta aagtccaaaa tatacaactc tacagtcagc tcccgtcctt
    64921 caccgaatgt atcattatgt ccaattgaac ccatggctgg ataccatgtt tccccaattt
    64981 ttatttccga cacataaaca ccttctttag gcaaatgaac cgtgctcttt actttgacat
    65041 ttgctgtagg gaatcctagt agtctgcctc gtgcatcgcc atggacaact accccttcta
    65101 cctcatatgt gcaacctaat agttttgctg cttcttttac ttgtccgctg tctagtaatc
    65161 cacgaatacg ggtagagctg attttttcac cttgttcttc ttctttagaa actgtgacga
    65221 ttttgaatct tcctttggca tattcaggaa aatgagcgac atctgcaatg tcttttggtc
    65281 catacgtata atcaaaaccg gaaacagcat attttgcatg taaccctacg atgtattgat
    65341 caacaaattc ctgcggtgct aaatgagcaa aagaagaagt aaaatcaact tcatataaat
    65401 aatctatacc caaagcggcc attttttctt ctttttgctt aaggctagtc aaatatttca
    65461 cgctgctagg atcgatcttt ttaaagacaa tagatggatg ctgattgaaa gtcatcaatg
    65521 ctaatttcaa tccttctttt tgtgcaattt ccatacctgt ctttatgact ttctggtgtc
    65581 cgatatgcac gccgtcgaaa aatcccagca ctaaaacgac atcgtcttct ggaatttgtt
    65641 ctgattggta aggatgtctg atcttgatga tattcattcg ttattcctca tttctcaata
    65701 ctttactagg ttttaatttg tttttttctt tcgggtttgg ctgataaata ctgacaactt
    65761 ttcctttata aaacaaagca atttcttctg acggcatttc tgggagcgca aatacagtat
    65821 aatcaagtct cataccgttt tttactttct gccatacttc atcagtaata tctactcgtt
    65881 tgaaccgttc cactcctttt tctattggca gcaaacaatc atcgattgtc ccatttttca
    65941 cagcttgagc tacttcttct aatgtcagag catccttctg ggacataccg gcacttgctg
    66001 ttcgggtcag atcagacatg tgcgcagggt aaccaagttt cttgcctgta tctacagcta
    66061 atgtgcggac ataggttcct ttcccgcatt ctacttcgaa gcgccaagaa atggttcctg
    66121 tcacttcgtc tcttgtcaat tcacttgtcc ggataaaacg gtaaatctga gcctttctaa
    66181 caggacgctc cactgtttca ttattgcggg catattcata taaccgctta ccgttgactt
    66241 ttaccgctga gtacattggt ggtatctggg tgatctcacc aataaatgaa gccatcactt
    66301 catctatttt tttttcagat aaagacatat ctactggttt tgtttctacc cgttctccgg
    66361 atcgatcttc cgtcgttgta ctgaaaccta gagttatctc gcctacatag attttcccag
    66421 aatctgtcaa atattcgata acttttgtgg cttttccaat acaaatgggc aacacaccgt
    66481 ctacgtctgg gtcaagcgta cccccatgtc cgattttttt cgtatgtaag atttttctta
    66541 atttgaatac gcaatcatga cttgtcattc cgcgttcttt ccataaaggt aataacccgt
    66601 ccatgtcttt tctccatttc atttttgatt taactgaatt attataacac attgcatcaa
    66661 ttactgacga atttgacatc ataatgaacg gtttttattt ttcggcaata aaaacgtaac
    66721 aaaattcatg ctcacgtaaa tagaaatcat gttatgcttt tttaaaggag ggaatagaat
    66781 ggaagatttt ttctttgcac catttgattg gatttatggg tggcttacag gtaaatagtt
    66841 tggttttcac ttcccaaaaa cgaaaaaaaa gactggccat tcttccagtc ttttttccgt
    66901 tttttttatt tattttttca gtttcttaat caatgtaaaa aattctattt tgcgggatcg
    66961 attttatcat tttattggtc aagatttttc catttccaat ccaatacttc tggcagatca
    67021 ataccatttt cacgaatata gttttgatgt tcttgtaatt tctgatccat ttttttcgca
    67081 aaatcttctg cttgacttcc tttgattttt aaagctgcgt cttttgccaa atgataacga
    67141 tcaagttcat ttactacacg catgtcaaat ggtgtcgtga tatctccatt ttctttgtag
    67201 ccatgaatca ttaaatgatg gttatgacga tcaaagaaaa tcgtgcggac caatgtttca
    67261 taaccgtgga aagcaaataa gatcggtttg tcttttgtaa aatattgatc aaattcttca
    67321 tcgctcaacc cacgcggatc ttgacttgga tgacgtaatt tcaataaatc tacgacattg
    67381 atgaaccgaa tttttagttt tgggaattct tccagcagca aagttacagc tgctaatgct
    67441 tctaagttag attccgttcc tgcagcagca atgaccaatt caggttcctc ctcttttgct
    67501 gtcgaagccc aatcaatgat tttcaagcct tcattcacta agacggctgc ttcttctgct
    67561 gagtaaaatt gagcacgcgg atgtttggaa gagatgatca aattgatttt ttctgacgag
    67621 cggaatgctt tatccatgac tgcgagcaat gtatttgcat ccgcaggcag atattcacgg
    67681 atgaactccg ccttcttttc tgccaaatgt gtcaatattc ctggatcttg gtggctatat
    67741 ccattgtgat cttgctggaa gactgttgag cttgcaatga gatttagcga aggataatca
    67801 tttcgccaag aaaggtcatg tgatttccgc atccatttga agtgctgagt caacatagag
    67861 tcaaccactc gcaagaatga ttcgtaactt gcaaaaaacc atgccgtcca gtcagaacgt
    67921 acccttctaa gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag
    67981 atggcgaaag ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg
    68041 ttttgaaaac ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat
    68101 tttctggatt tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg
    68161 ctttaacgga gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa
    68221 gcggtcttgg atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag
    68281 taggcgcgat ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg
    68341 gttcatagga tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta
    68401 caggaatcgg tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt
    68461 ttggaccggt ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga
    68521 catctgttgc tgtcttcagg cgagcttctt tttggatgga tttaattttc tcgattgctt
    68581 gatccatttt ttcagccata acaggattta atttttctga atcatttcct tcaataaaga
    68641 aaggttccca gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca
    68701 agatcgttgg gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat
    68761 ctatcactgg attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg
    68821 cttcaccatc accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga
    68881 ctgcatgtga aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg
    68941 gtgctgcatg agaagcaatg cctccaggaa atgaaaatcg tttgaataat ttttgcattc
    69001 ctgtctcatc ttccgttact tctggataga tctctgtgta acttccatct aaataagcat
    69061 tcgataccat gacttgtccg ccgtgtcctg gcccttcaat ataaaacatg ttcaaatcat
    69121 atttattgat gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc
    69181 aatgaccgat tggatgtttt ttgacatctt cgggtttcag tgtacgtctc agtaaggggt
    69241 tatctttaag ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca
    69301 ttttatcaaa gtattcttta ctagaataat ccatacataa cactcctttt cattattact
    69361 cttattctag cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg
    69421 gattttatat ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga
    69481 cgttttttac attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt
    69541 tcgctcctca tcatgcataa tttgctgaat gacgctgggc cagttaacac attgctggaa
    69601 aactggggct ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg
    69661 ttatctttgt caatatgtga attggtatcc cagctactat gctaatttct acagggatta
    69721 tccaaaattt ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa
    69781 cgtgattttt cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc
    69841 cactgatctt ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc
    69901 ttccgttatc ggtatcttga tctttatcta tatgcaaaaa tttatatact actagaaaaa
    69961 agaaagtcga attttttact tttctttttc tcttttttgg ttagctgaca cgccgcaaga
    70021 ctaaagtcat ggaactctta ggtaatacgt cttaatgaaa acaaaaaaat cccgcaaccc
    70081 ctgatttcac aggatttgcg ggagtttttg attagtcttt gttcaagtcg cgaagcaatt
    70141 catcgatatg gttgccatag acaactgatt catcaagttc aaagaacaat tcaggtgtct
    70201 tataaatact tagattgtgc cctaactctc gacgaatcaa tcctgatgct ttattcaatc
    70261 catcttgtgc tgttttctta tctgatgcta agtcagataa aatgctgtaa tagatcgttg
    70321 cttgctgtaa gtcccccgta actttcacat ctgtgatcgt tacgttctcc acacgagggt
    70381 cacggacctt tttgcgcaaa atatcattga cttcttttaa gatttcttga ccgactcggc
    70441 ggtcacggta gttagccata atgattggcc tccttttatc agtttttagt catttttgac
    70501 ttcttccatg ataaatcctt caattacgtc atctactttg atgtcattga atttttcaat
    70561 cattgcacca cattcataac ccattttgac ttctttcacg tcatctttga atcgtttcag
    70621 acttgcaagt tggccttcat agattacgat tccgtcacgg attacacgga caccgctatc
    70681 tctacgaata tatccttctg ttacataggc acctgcaatc gtaccaactt tagaaacttt
    70741 gaaggtttca cgaacgatca tttgaccggt gattttttct tcaaattcag ggtctagcat
    70801 ccctttcatc gcagtttcga tttcttcgat ggctttatag atgatacggt gaagacgaat
    70861 atcgacttct tcagtatcag cttgcacttt tgcctgcggt gtaggacgaa cgttgaaacc
    70921 gatgataata gcgttgctgg ctgctgctaa ggttacatca ctttcattga ttgctccgac
    70981 agctgaatgg acgattttta cacggacacc ttccacatca atcttcttca atgatgcagc
    71041 cagtgcttct gctgaacctt gtacatctgc tttgatgatt acgttaactt ctttcaattc
    71101 gccttctttc aggctttcaa ataagttatc caaagtcaca cggcttgtgg cagcacggtt
    71161 ttctaataat gcacgtttcg cgcgttcttc acccgctgca cgtgctgtct tttcgtcttc
    71221 gaagactacg aaacggtcac ctgcttgagg tacatcattc aagccagtga tttctactgg
    71281 cgttgcaggt cctgcttctt tatcgcgacg gccaagatcg tttgtcatta cacggacacg
    71341 tccgtaagta ttaccgacaa cgattgggtc accaacatga agtgttcctt cttggacaag
    71401 taaggttgac acaggacctt tacctttatc aagacgtgct tcgatcactg ttccgattgc
    71461 gcgttgtgtt ggatctgctt ttaaatcttc tacttctgcg actaacagga tcatttccag
    71521 caattcttcg atgttttggc cgaatttcgc tgagatctca acaaagatcg tttctccacc
    71581 ccatgcttca ggaattaact catactcact taattcttgc ataacatgtt gagggtttgc
    71641 acctggttta tcaattttat ttacagcgac gatgattgga acgcctgcag ctttggcatg
    71701 gttgattgct tcaactgttt gtggcatgac gccatcatct gcagcaacaa ctaagatcgt
    71761 gatatctgtg atacttgctc cacgtgcacg catacttgta aaggccgcat gtcctggtgt
    71821 atccaagaaa gtgattggtt taccatcaat atcgatctgg taggcaccga tatgctgtgt
    71881 gattccgccg gcttcgccgc ttgttacacg tgaatggcgt aatgtatcca ataaagttgt
    71941 tttcccatgg tctacgtgtc ccataatcgt tacgactggc ggacgtgaga ctaatttatc
    72001 tggatcgact tcttctgcct cgaagaattt atcgatatca gcaatgtcaa cttggacttt
    72061 ttcttgtggt tccatgccat agtccgtcgc taacagttca atcgtatctt tatccagtgc
    72121 ttggttttga ttgaccatga caccaagcat aaataatttt ttaatgattt ctgctggctc
    72181 acgatgaatt tttttcgcaa tatctgctac gttcatacct tcagtatact ctaatacatc
    72241 tggcaattca cggaatttgc gtggtggtac agctggttta gttgattgtt gctgtttgcc
    72301 ttttttacct tttttattat aacgattacg gttattatta ttaaatttgc cgcgattgtt
    72361 attgtttggt ttgttgttat tgttagtacg gttttgattg ccgccttggc tcgttccttg
    72421 acgattttgg ctgctggtat ttcccgtatt attttggttt gttgttggtt tttttccttg
    72481 attcacttga ccgctccctt gaccgcgatc ttgataactg cggttatttt tatttttttg
    72541 ttgattgttc gttgtttctt ttttacgttg atcgttcggc tgttgattgg ctggttttct
    72601 gcttgcttgt tgtgcagctt gttgtttcgt gcttgggttt ttgaacgctt gttgcaattt
    72661 cttctcatca ttgctagtaa tcgcacccat atggtttttc acatcgatgc caagttgttg
    72721 tgctttttcc acgacgtctt tactagattt tttgagttct tttgctaatt catagattct
    72781 tttgttgccc atgcaatcac cttcctaacc ttcaattagc tccttggtct tcttcgcaaa
    72841 tccggcatcc attattccga ataccttgcg aggttttccg atcatctgcg tgatttctgc
    72901 ttcagaaaat agctcaaagc aaggaacttc ataatatgaa ctcttatctt ttatcttttt
    72961 tcttgtattc tcgcttgcat cggttgccac aaacaccaat tttgcttttt gtttgcggat
    73021 ttcagcaatg gtcagttctt cacccgtgat tagttttccc gcgcgcatcg ctagaccgat
    73081 gagattcatg gctttttgcc gattaactgt attcattttc caaataactc tttacgtgct
    73141 ttttggtgag tgacatagtc aagaagttct tgataaaact catctgttaa tgttgtttcg
    73201 agtactcgat cgagaatttt tttatcccaa gcttgttgta cttcttccgg ctcaagtgag
    73261 acatacgctc ctcgtccagg catttttcca gttggatcga tcgatacttc cccttctttt
    73321 gaacgggtga ttcggaccat ctctttttta ggtttcattt cacctgaaac cacggatttg
    73381 cgtaaaggaa ttttcctttg tttcatctct ttgcctcctt actaaagtta agcttctggt
    73441 ttttcttcga ccgtttcatt gttgaatgca attgtttcat attcatcgtc tgttaaatct
    73501 gactgaacga ttgcttcatc atgcagttct tctgtttctt tttgttgttc ttgattttca
    73561 taaaattccg tcatgtcaga ttctgatttg atatcgattt tatggttagt caatttagca
    73621 gctaaacgtg cgttttgtcc gcgtttaccg attgctagcg ataattggta gtctggaacg
    73681 acaactgtgc aggcctttgg atttgtctca tcgaagatca catccatgac ttgtgcagga
    73741 ttcaatgcat ttgcaatgaa tacagctgga tcttcattcc attcgacgat atccatgttt
    73801 tcgcctttta attcattgac gattgcttga actcgttgtc cctttggccc gacacatgta
    73861 ccgactgcat cgatatttgg atcatttgag cgaactgcca cttttgaacg atcgccagct
    73921 tcacgggcga tgctgacaat ttcaacgatt ccatcataga cttctggtac ttcttgctca
    73981 aataagcggc gcaataggtc tggatggctg cggctaacaa atacttgcgg acctttagac
    74041 gtattttcta ctcgggaaac ataaactttg attcgatcat gtggttgata aaattcattt
    74101 ggcatttgat cttgtttaga aagaactgcc tcaattttac caagattgac ataaatataa
    74161 cgtttatcct ggcgttcgac gattccttgc atgatgtctt tttcataagc actgaattcg
    74221 ttataaataa tcgttctttc tgcttcacgt acacgctgta aaataacttg tttagccgtt
    74281 tgcgcagcga tccgcccaaa atcttttggt gttacttcaa aacggatcgt atcaccgatc
    74341 tcatatgctg gattgattaa aagtgcatct ttcaatgaga cttccaactg tgaatccatc
    74401 acttcttccg ttacttcttt taccgcatag acatggatct ttcctttttt ctgatcaaat
    74461 tccacttcga cgttttgtgc ttggccatag tgacgtttgt atgcagaaac caatgctgct
    74521 tctaaagcat cgatcacgat ttcttttgag atcccttttt cagcttctaa agcgtctaac
    74581 gcatttaaca tttctttact cattttttct gattcctctc cgtgattaaa attggatagc
    74641 taagcgagct tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat
    74701 gcgtattttc aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt
    74761 ttcgccatct actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata
    74821 gtcgctttct tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc
    74881 ttgaggaatc ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa
    74941 ggcgcattct tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct
    75001 ttttccttct tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg
    75061 tgtcaccatt tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaaa
    75121 ttaagtgcgt aattccatta aaaagagtga gcgaaaaatc cgctcactca cagttagtat
    75181 cattacctat aagaactata ccatactctg cttaaaatta caagcagaca ggaaaacaga
    75241 tgcttatagc atatcgaata acgataattg attttcatct ggtagatctt tcaatacgcc
    75301 attgtcgttc atatattcga tcaatgtttt tgaaacttta ccgcgggtgg caagatcttc
    75361 tttggataaa aatggaccgt cttttcttgc ttcaacgatt tgttttgcca cgttggttcc
    75421 caaacttggg acagctctaa atggtgcgat caacgtatca ccttcaataa caaagttaac
    75481 ggcatccgat ttatacaaat caatcattcc aaatttgaat cctcgttcca gcatttcatt
    75541 ggctaactcc agcactgtca gttgattctt ttcttttacg gaagcatcta atcctttgtc
    75601 cgtgatttct ttcattgctt gtttcacagc atcttttcct ttgcacatgg agactagatc
    75661 aaagtcgtct gcacgtacag aaaagtatgc acagtaatac aaaataggga aataaacctt
    75721 gaaataagcg acacgaagag ccatcaatac gtaagctgca gcatgggctt tcggaaacat
    75781 atatttgatt tttgaacaag aatcgatata ccagtcgggc acattatttt ctttcatcgc
    75841 cgatagatag gtttctcgca attcatcagg gatcttgttc cattgtcctt tacgaacagt
    75901 ttccatgatt ttaaatgcca tcccgctatc taatccagca tggatcaaat aaaccataat
    75961 atcatcacga cagccgatta cttccgccag tgtcgcatct cctcgtcgaa tcaattcttc
    76021 cgcatttcct aaccatacgt ctgtaccatg agatagtccg gaaatttgca gtagttctgc
    76081 aaaagttgta ggatgcgttt cttctagcat ccctcgaaca aaacgagtgc cgaattctgg
    76141 aatacctaat gtccctgttt tggaataaat ttgttcctga cttactccaa gtacttcagg
    76201 accggcaaat atccgcatca cttccgggtc atccgtaggg atcgtttgtg gatcgatccc
    76261 tgataaatct tgaagcatcc gaataacagt tgggtcatca tgccctagaa tatctaattt
    76321 caatacatta tcgtgaatcg agtgaaaatc aaaatgagtc gttttccatt ccgaattttg
    76381 atcgtctgct ggatattgga tcggcgtaaa atcatagaca tccatataat cgggaataac
    76441 gatgatcccc cctggatgct ggccggttgt ccgtttgact ccagtagcgc cttttgctaa
    76501 tctgtcgact tctgcacttc gaaactgcag attattgtcc ctttcgtatc cttttacata
    76561 gccatatgct gttttgtcgg cgactgtacc aatcgttccg gcacggtaaa catagtcttc
    76621 gccaaacaat actttcgtat agttatgggc ttctgcttga taatcacctg agaagttcaa
    76681 atcaatatcg ggcactttgt ctccatggaa tcctaaaaat gtctcaaatg gaatatcatg
    76741 accgtctttg tttaagcgtg caccacattt tggacattgt ttttctggca tgtcaaatcc
    76801 agaaccatac gtaccatctt caaaaaactc ggaatattgg cactctggac aataataatg
    76861 aggggctaac ggattaactt cagtgattcc tgtcatcgtc gcaacaaagc tagagccaac
    76921 agagccacgt gaacctacta gatagccatc ttcattactt ttatgcacta gtttttgggc
    76981 gatcagataa ataaccgaga agccattacc attaatagaa ttcaactctt tttttaatcg
    77041 tttttgaatg atctcaggta acggatctcc gtacatttgt tttgcttttg tgtaactcaa
    77101 ttctgatatt tcatcttctg aacctggaat cttcggtgta taaagttcat ctttgactgg
    77161 aatcacttct tcgcagatat ctgcgatttt attcgtattt tctactacga tctctttcgc
    77221 tgtttcttcc cctaaaaagt gaaatgcagt tagcatctca tcggttgttc ggaaatggac
    77281 atccggcaaa ctatgacgat tcaacggatt cgctccgccc atagaattga tcaaaatttt
    77341 tcggtaaatg gcatcttctt cgttcagata atgtacatta cctgttgcaa caacgatttt
    77401 atccaaactt tttccgattt ctaccaaatt ttggataatt tcttccaaat catgttcatt
    77461 ttttaccaat tcttgctcga tcaaaggcgc atagaccgct tttggcataa cttcaatata
    77521 atcgtagaat tttgctcggt ttcttgcctc ttctacccct ttttgcatca tggcttcgaa
    77581 tatttctccc ttatcacaag ctgagccgat caatagattt tcacgcatct ttttcaattg
    77641 ggagcgtggt attcgtggga cgcgttcgaa gtattcgaca ttcgacattg aaatcagttt
    77701 gaacaaatct ttcagtcctg cctgattttt tgctaaaatc gttacatgga aaggtcgagc
    77761 ccttttgtaa gaatctcctt ctccgatatg ctcatttagc tgatcatgat aaagcatgtt
    77821 atgattgtcc attgcctctt tcacaaagat ccatgctaag tgaccagttg cttcagcatc
    77881 atagattgct cggtgatgct gttccaagct gacaccaaat tttttagata acacacctaa
    77941 tccgaaacgt ttgaattgag gatacagata tctagctaat tccaacgtat caatgactgg
    78001 attggccgct tcaggaatac cataccttgc gtagctagta ttcaagaacc ccatatcaaa
    78061 agccgcgttg tgggctacca aaattgtatc ttttgaaaat tcaaggaaca tccgcaatac
    78121 ttcttcttcc gactttgatc ctcgaaccat tccatccgtg ataccagtca gatctaccgt
    78181 tgttcgtgac aaaggatgtc ctggatcgat aaattcatca aagctttcga tcacatttcc
    78241 tttatacatt ttgactgcgg ctagctcgat gatcgtgtca tatactgcag acaatcctgt
    78301 cgtttccacg tcaaatacga catatgtggc ttcactcaat gcttcatgtg catcattata
    78361 agcaattggg acaccatcat ctacgacatt cgcttccacg ccgtataaga ttttcacacc
    78421 tgcttttttt ccagcactat gtgcttccgg aaaggcttgc gcaccaccgt ggtctgtaat
    78481 ggcgatagct cggtgtcccc atttccctgc ttgtgccaca agatcagaga tgctgtttgt
    78541 tgcatccatc gtactcatat tgctatgaac atgaagctct actcgttttt ccccttcagg
    78601 agcataatct ttgcgaggtg tatgtttgac ttctatgata tcttgcgcat tcatgaccaa
    78661 atcacggaca aacgtatctt cttgaatact tcctcgtacc tttaaccagc taccgacact
    78721 gatcgcatca aatacttgtt catctttttc gccgttcgag aattttttga cgataaatga
    78781 tgaagtataa tctgtgattt tcaaagtcaa gatcttgcgt tttgaacgta gttctctgac
    78841 ttctttgtcg aatacgtacc cctcaagtgt gactcttcgt tcttcttcaa cgatactgat
    78901 cattggtgtc gttggttcat cggctggaat atttcgtcct agctggatgt ggatgggtcc
    78961 ttcaagagct ggcgattgct cttttctttc ctttttcttt tgttcgtgga caactagact
    79021 ttcagctgct tgcttcataa aggcttctgc ctgttcttgt ttgcgttctt caaaaagttt
    79081 caatactcgt tccgcttgct gctcatccac ttctggttcg atacgaaatt ttgggaatcc
    79141 gtaactgacg aacaactctt ctactaatgg caagtattgt tgcttcaaat aagaaatagc
    79201 tcctgtactg tcgattggca agatgacttt acgatctttt atcacaggta cctgcgtttt
    79261 cagtacttgc tgtgctagtg gtgtatcgca ttgctgattt tccaaagctt gggcccagta
    79321 atcttgcaag agctgttcat cgaatgtctg gtcatctgct aagatattca gtttgacttg
    79381 tgcaatatct ttaaaagcga tagtcagatg ctgagtcaat gaccgatata gcataatagg
    79441 caaaattttg gaaaaatgca gcgtaaattc ccataaacgc gatttacgat ggaccactac
    79501 actatccatt tcaccagcag taagcagtgg atgatttttt tctgcttcct ctagttggat
    79561 ttgttccaac agtttttcaa ataaatctcg tttttctgac aaagatatac gccctttctg
    79621 atgagaaaca atgcaattga tcgcatctta tcctcttctt tttcagtacc ttttagtata
    79681 ctcgtttttt ctgagtttta cattaaaaaa ttggaactgc gattcaaatc gcagttccaa
    79741 ttttaaaagc gaatcatcag attgtcaaaa acagctgatt cagctatttt tattttctgt
    79801 gttttaacgc ttattattcg ccttggttca agaggattgg taaagtattc accaattctt
    79861 ctttacgtac ttcaaccatt tcgccagttt tcttgatttt cacttctacg attccatcca
    79921 ctgctttttt accgacagtg attctgattg gacagccaat caaatcagaa tccgcgaatt
    79981 tcactcctgc gcgttcattt ctgtcatcaa ccagtacttc atagccagct tttgtcatcg
    80041 cttcttctag ttcattcgtc aggtttgttt ggtattcgtc ttttagattc atttggacga
    80101 tatgcaaatc aaatggcgca attccttttg gccagttgat tccttgttca tctgaatgtt
    80161 gttctacgat tgctgataat aaacgactta caccgatacc ataacagccc ataatcacaa
    80221 atttttctcg tccattttcg tctagtacag ttgctcccat agattcactg taacgagtgc
    80281 cgattttgaa gatatgtccg atttcgattc ctcgagtaaa ggcaagaaca ccattgttgt
    80341 ctggtgaagg atctccttct tgtacaaaac gaagatcttc ataagaaagt acagtgaaat
    80401 cgcgttctgg attcacattt ttatagtgga atcctgtttc gttcgctcca gtaatggcat
    80461 tcgctaaatc ttggacataa cggtctgcat atacttttac gtcatctgaa acaccgactg
    80521 gtccgacaga accaaactca gcacctagat atttttgtgc ttcttcttct gttgcttctt
    80581 ctaaaaagtc agcagataaa aagtttttca gttttacgtc attgacttca tggtctccgc
    80641 gtaccaaaac aaggacaggt tgttcatccg caatgaacaa tacagacttg atgatttttt
    80701 gaggttcaac agaaaagaaa tctgctactt cgtcaattga ttttacgtta ggtgtagcta
    80761 ctttctccaa ttcaagctgt gtttcatgcg atttttttga tacatagtag cttgtagcca
    80821 tctccaagtt tgcagcataa tcactttcag tcgaataaca gatcgtatcc tccccgattt
    80881 ctgagatcgc catgaattct tttgagtctt tcccgcccat tgcgccaccg tctccgataa
    80941 tcgagcgaaa ttctaagcca caacgcttga atacttcggt gtaggctttt tcgtaatctt
    81001 tatatgtctc gtccaaactt tcttccgttg catggaaaga atacgcatct ttcatgataa
    81061 actctcttcc gcgaagcaaa ccgaagcgag gtcgtttttc gtcacgatat ttaggttgga
    81121 tttgataaag atttaaaggt aatttcttat atgaattgat ttcattacgc atcaaatcag
    81181 taaaagtttc ttcgtgagtt ggtccaagga tcatttttcg atcattgcgg tctttgaaac
    81241 gatacaagtt tggtccatat gtttcgtaac gcccggattc ttcccaaagt tctgctggca
    81301 agatggcagg catcagcatt tctaccgctc cgattttttc aaattcttcc cgcataatgc
    81361 gtttcagttt ttccaataca cgatttgcta atggcagata tgaataaata cccgctgata
    81421 cttggcggat atatccagct cttagcaaga tttgatggct taatacttct gcatcatttg
    81481 gaacttcgcg taatgttgga atcaacattt ttgattgtct cattctatat actcctttat
    81541 ttcctcaaaa tagttatcgt ttgttattat accctatttg tcaacttttt ctagaaaaag
    81601 aatcgttgaa tatcgttcca tgtaactagc accatcaaca acattaaaaa tccaaagcca
    81661 gctaatgtca atattccttc tttttcttga cttaatggtt tcccgcgaat cccttcaaaa
    81721 atattcaaga ctaatttccc gccatctaag gcaggaatcg gaagcagatt gacgattccg
    81781 agattcattg aaagaagcgc catcaaacta ataacagttg ttacaccttg atttgctgct
    81841 tctgaagaca attggtacat catgacaggt ccgcctaatt tatctaaact gaaacctgta
    81901 aatagtgatc ctaaggcttt gaaaatttcc aatgaaccgc tgaaagcttg acgcgtccct
    81961 ccaatgatct tatccataaa acctgtattc attggggctt ttataccaag ttggccaact
    82021 ttttcgccat ttgactctac agatttcggt gtcacgtcga cagattgcac ctgaccttct
    82081 cgttcgatct taaagtctaa tgttttatcc ggatttttcg taatgactgt tgtcagatca
    82141 ttccatgaat ggatttcttt tccgtccaca ctgactacct catcattttc ttttaagcct
    82201 gcttctgccg cagctccatt aggcatgatc tctcctacgc gattagtgtt cgtcacttga
    82261 acaccacctt gcataaatgc cagaatgatg aataacagga ttgccaaaat aaagttattc
    82321 atgggtccag caaagttggt cagcatacgc tgccacaatt tagccgattg gaactgtacg
    82381 tcttttggcg caattctgat ttctgttccg tcgttttcaa taatcgtcgc atcgtgatca
    82441 acagggtagg ttactacttc tgtttcgtct ccattcacat aaccagtgat agtcagctca
    82501 tcttctaaat cataacggct caattccatc gggatagcat ttgtcaattg gattttctta
    82561 cttaaattta tcttctctac gatgccgtca gaatttagta aaagagaaag aggcattcct
    82621 ggtgccattt ctgtttcatc atccccattg cctgccattc gaacataacc gccaataggt
    82681 aataggcgaa gcgtatatgt tgtcccgtct tttgcttgat gcccataaat tttcggaccc
    82741 ataccgatag caaattcgcg aacgaggatt cctgatcgtt tcgcaaagaa gaaatgacca
    82801 aactcatgaa caatcactaa tataccaaaa acgatgataa atgtcagaat cgttttcata
    82861 gcttttttct cctttacttg ttttttgtgt tttttataaa acaacgataa aacactataa
    82921 ttttaccata gtataccaaa gacaccaata gtatcaatat aatgaaaaat aaaaacctgc
    82981 gtcttttatg aaattcacaa agaaacgcag gtatacttac taaataatgc caaataaatg
    83041 catgatagga aatacaaaca gcaaactgtc gaaacggtct aagattccac catggcctgg
    83101 taagatattt cccgagtctt tcacatcata atgacgtttg attgcagatt cgaccaaatc
    83161 accaaattgt ccgactgctg aaaaaataat cgtcatcaga agcatgacaa acataccatg
    83221 attaaatagt tcttttgctg ggtatagcaa taaataaaga aatgcgacta caacagcact
    83281 accaatacca ccgagcgcac cttcaatcgt tttgttgggc gaaatatcag gacttaactt
    83341 atgctgacca taacgtcgac caaccatata tgctccgata tcagttgccc aaacaataaa
    83401 tagaccaaat atcaaaacag ccaagctttc tgttcgtgca ttgacaaaat tctggaaacc
    83461 tattcccaca tacaagctga caagcacagg aaaacctgct tcatcaatcg tatacgtatt
    83521 tttagaaata actgatgttc ctaaaagaat cataactata agataaaata acataaatgt
    83581 acttacattc tctggaagaa agaaaaacca tctttctttt ggcaaaacaa gtaacactgc
    83641 accaacggcg gataaaactc cttcaaaact taagagtgtc aatcctttca tccgaaacag
    83701 ctcgtaaact cctattactg ctaagattgc tgctgtaagc tcaaccgcta tgcctccgat
    83761 ccatataatc ggaataaaca agactagcgc cacggctgct gtgaccacac gttgtttcat
    83821 gactgatccc ctttctcatc ttctgttttt tttacaccgc cgaatcgacg gtctcgattt
    83881 tgataagagg cgattgcttc ttcaagatgc gcaccatcaa aatcaggcca aagcgctttt
    83941 gtaaaataca gctcactata tgcgatctgc cataacagaa aattactaat tcgctcttct
    84001 ccacttgtac ggatcatcag ctcaggatct tgtaactctt taggcaagaa acctgtcatt
    84061 aaatgcttag caatcaattc atcatcgatt tcttctgcgt gtatttctcc ttttgctact
    84121 tcttcggcaa tttcttggac agcagtcacg atctctgctc tgcttccata attcaaagca
    84181 aaattcaata ccattccagt attgtttttt gtttgctcaa ttgcacgtct tacagcatct
    84241 tgcgtatgtt ctggcaatac gttttcataa cccatgacat gaactttgac attttcttta
    84301 atcaattctg gtacgaacgt atcaaagaag tcgacaggta gttgcatgag aaaacttact
    84361 tcatcttttg gtcgcttcca gttttcagtc gaaaaggcat aaagcgtcaa cactttcact
    84421 ccaagccttg atgctttttt tgtgactttc tttactgttt ccatgccttc tttatggcca
    84481 gcaactcgtg gcaaacgacg gttttgcgcc caacgaccat tgccgtccat gatgatggca
    84541 atgtgctgag gaattggtcc atcggtatta aattgaaatt cgcttttttc ttgaatatat
    84601 ttgttttttt gcggaaaaaa acgtaacatt ctttttcctc ctatacttaa aagattccca
    84661 ctatcttaag tattatagca atatctaact gtttttccta tgaaatcatt gtttttttat
    84721 ttaaaaactg aataattttt tcttttttta atttttaaat aaaagaaata taaataaaaa
    84781 cagaggaagc ggaatgatcc gcccctctgt ttttatgatt ttattaaact tccaataatt
    84841 ctttttcttt atcagcagcg atcttatcta cttctttgat gctgttatct gtcaatgttt
    84901 gtacgtcttt ttctaatccg cgcaaatcat cttcggtaat atcaccattt ttttgttgtt
    84961 ttttatattc atcgatcgca tcacgacgga tgttacgaat cgcgattttt gcattttctg
    85021 cttctttttt aacatcttta gctaattctt ttctgcgttc ttctgtcagt tgcgggataa
    85081 ctaagcgaat gacatttcca tcattggttg gactgattcc gatatcactt gccataatag
    85141 ctttttcaac atcttgaaga atgctcttgt caaaaggtgt gatcattaaa acacgtgctt
    85201 ctggaatttg gattgatgcc atttggttca atggtgttgg cgcaccgtag tagttcacag
    85261 taatacggtc caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt
    85321 gaagattttg tgctgctttt ttcatttttt cttttgcttc tgtcataatt gcatctgcca
    85381 tttttatttc ccccttacag ttgttccgat attttcgcct aagattgcac ggcgaatatt
    85441 tccgtgttca tttaaattga agacaagcag tggaatatca ttatccatgc ttagtgaact
    85501 tgcagttgaa tccatgactt gtaatccttt ggcaataact tccagatgag tcagttcttc
    85561 aaattttact gcatttgcat ctactttcgg atcagcagaa taaacaccgt ctacattgtt
    85621 tttagccatc aggataacat ctgcgccgat ttctgccgcg cgtaatgctg ctgttgtgtc
    85681 tgtcgagaag tacgggttgc cggtaccgcc tgcaaagata acgatccgtc ctttttctaa
    85741 atgacgttct gctttacggc gaatatatgg ctctgcgatt tgacgcattt caatcgaagt
    85801 ttgcacgcgt gtcggtacac ccacgttttc caacgtatcc tgtaaagcta aggcattcat
    85861 aactgtagct aacataccca tgtaatcagc ttgagcacgt tccatgccca tttgtgctcc
    85921 gatttgtccg cgccagatat ttccgccgcc cacaacgatt gccatttcaa cgcccagctc
    85981 gtgaacttct ttgatttctt ggatgatttc tttgattact ggtggtttga ttccaaatcc
    86041 tgcgtcccca gctaaagctt ctccactcaa ttttaagact acacgctgat atttcggtgt
    86101 gaccatgttc attcctccaa tattttctat tgccatttta ccatacttag atgatttcga
    86161 cactctaaga ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt
    86221 cccgtctgtt attcaacaga attatttttt cacttggctc ataacttctt ctacaaagtt
    86281 atcttcgcgt ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt taacagttgc
    86341 gccttttgat gcaacgtatt tttcaacagt catatcagga tctttaacga atggttggtc
    86401 caccaaagcg atttctgctt tgaatttctt caagcgtcct tctaccattt tttcaacgat
    86461 attcgctggt ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt catgttctaa
    86521 ttcagcttct ggaatttgtg attcgtttac ataacgagga ttgatagctg ctacgtgcat
    86581 agctacgtct cttgcaactg tttcgtcagt tgttccatct agaacagtca atacggcaat
    86641 acgtccgccc atatgcaagt atccaccaaa tgcagcattg tcgtcttttt ctacaacttc
    86701 aaagcgacgg aagctgattt tttctccgat aacttgagtt gcttcgatca agtcagattc
    86761 aattgtacct ttatctgttt tgattttcat tgcagcttcc atgtctgctg gtttgttttc
    86821 agcaacaagt tctgcaattt cttttactaa atcttggaac atttcgtttt tagaaacaaa
    86881 gtctgtttct gagtttactt caacgattgc tgcagtgtta cctttcacag caactgaagc
    86941 caatccttca gctgcaatgc ggtcattttt cttcgcagcc tttgccattc ctttttcacg
    87001 taaaagatca actgcttttt ccatgtcgcc ttctacttca actaatgctt ttttcgcatc
    87061 catcattcct acgccagtca tttcgcgtag ttcttttacc attttagctg taacgtctgc
    87121 catgtttaac tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga
    87181 ggcttgtcgc ccagcctttg aaacagctcc atcattcaaa attattctgc tgaagaattg
    87241 tcgccttcaa caacatctac gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa
    87301 tcttcttcaa ctacttgatc ttcaccttgg tttccttcga tgaaagcatc agccattttt
    87361 gaagtaatca atttaacggc acgaattgca tcgtcgtttg aagggattac tacgtcgatt
    87421 tcatctggat cgcagtttgt atcaaccata gctacgattg ggatatttaa tttgtgtgct
    87481 tcttgaacag caatacgttc tttacgtggg tcaacgatgt acattacatc tgggattctt
    87541 ggcatatcag cgataccgcc taagaatttt tcaagacgtt cacgttcttt gttcaaacca
    87601 gcaacttctt ttttaggtag aacttcaaaa gttccatctt cttccattgc attgatttgt
    87661 ttcaaacggc tgatacgttt ttggatagta tcccagtttg tcaatgttcc gcctaaccaa
    87721 cgatggttaa cgaagtattg tcctgcacga gttgcttctt ctttgattgc ttcttgtgct
    87781 tgtttttttg tacctacgaa taatgctacg ccgccttctt cagcaacgct cttcatgtaa
    87841 tcataagctg catctactaa tttaactgtt ttttgcaagt caatgatgta gattccgttt
    87901 ctttctgtga agatatattt cttcattttt ggattccagc gacgagtttg gtgaccaaag
    87961 tgtacgccgg cttctagtaa ttgtttcata gaaattactg ccatgtttgt ttcctccgat
    88021 ttggttttat ttttcctctt cttatcttct accttgccgg caaacttttt caagcaccta
    88081 ccgacaatcc aataagaatg tggatttgct gatgcataaa gcaccctcag ctattataca
    88141 gacaaaagat ccgtatcgca agagcaaata tacttttctg ctcaacattc aatagtttac
    88201 ctgaaagata aaaagaattc aagaaaaaca gtagatttct ctttgtttct ttattatcct
    88261 ttttccataa aataatttta ggtataaaag acgaatatta ttgggcaatc cttctttttt
    88321 ttgttatgat acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga
    88381 catttgctga ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga agaactatta
    88441 aacaaacttg ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg agatatccgt
    88501 gatctaaaaa tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt attgtttcgc
    88561 gaaaaaggaa ctcctgagac taggactgag gaagaggatc ggctggttca aatgattgaa
    88621 gacattgtat tgaaagtcga acgagtccac tttttgacga tcgtcaatac cttgccagac
    88681 aatgctcatt tatttgcatc tgttttagat gagatcaaac ctccctctat tgtcagtaca
    88741 attgctggat tcgatacgat catggtcatc tcggaaaatg aggaggacgc aaaacgagta
    88801 gaagaatttc ttcaccatcc aactgaagta tcactttttt aatcagagca cgtattccta
    88861 atatggaatg cgtgtttttt tattttcaat attttcctta ttgaataaac ctttacactc
    88921 tttacaaaaa tacatcattt tttattcaaa tttttcaaca ccttgattga atcacaataa
    88981 tcatcattta tattggatgt taacaagagc agcttcttgc tctttgtgta tttcaaaaaa
    89041 gaaatgaaag gaaatcatat gtataaatta ttaacttctg gaccattgac gacttctgaa
    89101 acagtgaaaa atgctatgct gatcgatcat tgtacttggg atcaagaata caaagaaatt
    89161 acacaatgga taagaaaaaa attattggat cttgctcaag tatcggaagg aatatacaca
    89221 acggtcttaa tgcaaggtag cagctccttc ggggtagaat cggtattaag ttctgtgatc
    89281 aggccagaag atactgtact gatttgttca aacggcgcat atagacaacg aatgatcaac
    89341 atgtgcgaat gtctgaaact gaactatacc gtttatgagg tatcggaaca tcaaatcctc
    89401 caagctcatg tgattacaca gctgattgaa gagaatccgt ctatctccgc actagcaatg
    89461 gtccatagcg agactacttc cggtattttg aataatttgg aagagattgc tgctgtcaca
    89521 caagaaagag atcttttgtt tatagtggac gcgatgtcaa gctttggtgg gataccgatt
    89581 tgtgtagccg aactagggat tgattttttg atttccagct caaacaaatg tattcaaggt
    89641 gttccaggct tttcttttgt acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt
    89701 agccgaagtt tgtcacttga tctatatgac cagtaccgcg tgatggaaaa cgagaatggc
    89761 aaatggcgtt tcacttcccc tacccatacg gttttggctt ttcaacaagc gctgaaggaa
    89821 ctagaggcag aaggaggaat cgaaactcga tattcacgtt atctcgccaa taatcatgtg
    89881 ctacgacaaa aaatgacaga gatggggttc cagcctttca ttcaagaata tcaggggcca
    89941 ttcatcacct cttttcttta ccctaaggac tggcgatttg attttaatca tttttatcag
    90001 tatttaaagc aaaatggatt tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt
    90061 cgtatcggaa atattggcga gatctatccg gaagatattt tggaactgac agcaattatc
    90121 aaagactata aggagaacgt taatgattaa aactgtaatt tttgattggg caggaacaac
    90181 tgtggacttt ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg
    90241 aatccaactg actgacaaag aaatccgcta accgatggga aaattaaaat gggatcatat
    90301 ccaacagctt ctttccctcc cttctgtcaa aaaacaatgg atagctattt atggacaact
    90361 acctcaggaa aaagaggtgg aggaattgta tcaacaattt gaacattatc tgtttaaaga
    90421 tttggtcaag cattctacct tgaaacccga cacattggaa acgatcgaac ggttgagaaa
    90481 cgctgggatc aatattggtt ccacgaatgg atttaatgct cctatgatga caatcgtagc
    90541 agaaaccgca aaaaaagaaa ggtattcacc agacttcatg gcaacttttg aagacgtcga
    90601 agggtatggt cgaccgtttc cttatatgat ccataaaaac atgcagtact tcaaaaacaa
    90661 gtcggttgat gaagtaatca aagtcggaga tacggtctca gatatccagg aagggaaaaa
    90721 tgccggagtg ttgacagtag gtgtaattga aggtagttcg ctaatgggga tttcttatga
    90781 tgagtaccaa aaattagaga tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga
    90841 ttccaacaag ccggcgctga tatttgtgta aacaatttaa aagagttaac agaatacatt
    90901 ttgtacgctg acatgaatcc agaatgactt ttatacaaaa taagaggttg tgaaaaagct
    90961 gtcctgtatc aagtaataaa aacagccaag caaaaatagc tcctcatatt tttcgtttgg
    91021 ttaggcttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga
    91081 gctgtgacac gacttttgtc acagtcccct tatttgcaaa caaacagtgt ttaagagaca
    91141 acctctccac taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa
    91201 aatttgaaaa gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca
    91261 cattctcttt ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt
    91321 tcaataatac agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag
    91381 ctaaaatctt cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt
    91441 tcgcaatcac tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt
    91501 gaactaattt aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg
    91561 aagggacaaa tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat
    91621 aaggtccagt ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca
    91681 gtcgatcgtt tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag
    91741 catctttgtt ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg
    91801 cttcatttaa tacttcttct aataagacga tctttccttt acgagttgat aattttttac
    91861 caccttgcgt gatcaaacca aatggaatat ggtgcatgtc gtcagaccag tcaaagccca
    91921 tttccttcaa gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat
    91981 atagagattg tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg
    92041 taatgtatag tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag
    92101 ataggtcaac gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt
    92161 tgacgatttc atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta
    92221 cttccagcag atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc
    92281 ataattgaat agcctcttta tcgccttctt ccagacgttt gaaccaagca cgagcttctt
    92341 catttaattc tggttctgtt tcagctactt catggaattg gacgtataaa cgaagcaatt
    92401 cattgattgg ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca
    92461 gttttccaaa ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga
    92521 ttttttccat aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg
    92581 agattggttt ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga
    92641 tgttgctatc cccataatgt tctttttctt tcactactgt ttgcaaaact tttttgctga
    92701 taagttcttt attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt
    92761 tggcagaatc gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa
    92821 cttttgctaa tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt
    92881 ctagtaattg ctcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt
    92941 ttgcgacaag atctttatta ttcatcttta ttcctcctaa agtgttccct tgattgttaa
    93001 acaaaaaaaa tctctagcga cggcatactt agtatgccat cactagagac gagatttccc
    93061 gcggtaccac tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc
    93121 gtctgtttaa aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca
    93181 ccacttccga attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag
    93241 tcttattaaa ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa
    93301 aaaaacaatc cagatggcct aaaggagaag aaataccacc tggatagcta taaatcattt
    93361 cgtaaatacg aaagacttaa caaattaatc agctacgata atcgttcctt cttcagaagc
    93421 taaaagattc tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc
    93481 aacaaatgca atagctgctt ctactttcgg aagcatgctt cctggagcga attgcttttc
    93541 atcaatgtat tgtttcattt ctgaaacagt aacagtttct aatttctttt gatctggttt
    93601 ttggtaattg acatacacat tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat
    93661 gatttcggct aatttttctg aagcaaaatc tttatcgatt acggcttctc gtccttctaa
    93721 tccagtagct gtttctacaa caggaatccc accgccacca acagagactg tgataactcc
    93781 ttgatcgacc agcgtttcga tgacacgtgc ttctttaatc gagatgggtt taggggaagc
    93841 gactactttt ctccatccac gtccagcatc ttctttgaat gtcacagtgg agtcagcatt
    93901 catttgttct ttggcttctt cttccgtgta gaatggaccg actggtttac ttggattttt
    93961 gaatgaagga tcattttcat ccacaataac ttgagtgact aaagaaacca catctttatc
    94021 gatccctttt tccttcagta cttcgcccat ggcgttttgt aaccagtaac cgatagatcc
    94081 ttctgtcatt gcaacacatg tatctaaagg cattgccggt gttttctcag aatcagcagc
    94141 ttgctgctga atcaataggt taccgacttg cggaccattt ccgtgggaga tgatcaattc
    94201 atctccttgt tcgataaatt tcactaaata ctttgctgtt tccattaacg cttcttgttg
    94261 ggcttttgca cttgcatccg tagacaagat ggcattgccc cctaaagcga caactacttt
    94321 tcgtttggcc atttcattcg tcctttcttc agttctaccg aattttctag ataacaaaac
    94381 gagccagcgt tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg
    94441 ttatgcttaa acgcgaggga taaacaagtt acctaatgtt gctgccatga tcgctttgat
    94501 tgagtgcata cgattttctg cttggtcaaa ttgacgagca tatttgctgc ggaagacttc
    94561 atctgttact tccatttcag tgatgccaaa gcgttctttc atttgttcac cgtaaacagt
    94621 gtttgtatca tggaaagctg gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt
    94681 tttttctacc atttccatat tgatttggta aggtttcaac aatttgatac gttcttcgaa
    94741 tttatcttct tcacccattg atacccatac atcagaataa aggacgtctg ctccgtctac
    94801 acctttatca acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag caaagccttc
    94861 tgccattttg acgatttctt cttcaggttg taattctttt ggtgcaacga tacgcatatt
    94921 cgctcctaaa attgcacctg tgactaataa agagttagcc atattgtttc gaccatctcc
    94981 gcagtaagca acagtgatac cttctactgt tccaaagttt tcttggatag ttaaaaagtc
    95041 agcgatcatt tgtgttggat gccattcatc agtcaagcca ttccaaactg gaacaccaga
    95101 gaattctgct agttcctcta ccattttttg actgaatcca cggaattcga ttccgtcaaa
    95161 catacgtcct agtactttag ctgtatcttc tgtggattct tttttaccta attggatatc
    95221 atttgcacct agatattcag gatgtgcacc tagatcgatc gctgcagtag taaatgcaga
    95281 acgagtacgt gtagatgttt tttcaaataa aagcgcaata ttttttcctt ctagatagtg
    95341 atgtgggata ccacgttttt tcaagtcttt caagtgttct gaaaaatcga tcaaatattg
    95401 taattcttct tttgtaaagt ctttttctgc taataagctt cttccttgga atactgattc
    95461 tttcatatta aacattctcc tttagattgt ttttatttaa gattaaagag gaaatcccga
    95521 cggcagctga cagtaagact gccgtcagga aacaaattac ttcaagtctt cacggacaag
    95581 tggttggctc atgcagcgtg gaccaccacg acctcgtgac aattcacttg aattgatttc
    95641 taacactttg atgccatagc tacgaagcaa ttcgttcgat acataatttc ggttataagt
    95701 gactacaaca cctggagcga ttgccaatgt gtttgatcca tcattccatt gttctcgagg
    95761 tgcaacgatc tcatctccat taccagttgg gatcagtaca agatcatcta atccaagagc
    95821 agctttcatc gtttcgtgta agttatctga atgagtaatc ttgatatcat caccatctgg
    95881 ttcgattgta aagacatcca tcttaccgtc tttactttga attgctggat ggatagtgaa
    95941 tttatcgtga tctaccattg taaatacggt atccaaatgc atcattgcac ggttgttcgg
    96001 aattttgatt gccaacactt tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa
    96061 tttttctaaa gctttcgcat tagttcgttg agaaatccca acagctacta ctttatcgct
    96121 taagatcaat tcgtctccgc cttcgatatg atctggattt tcacgatcca accatacttc
    96181 gattccttta tttgcaaatc ttggatgatg tttcaaaata tattctgtaa acatcgattc
    96241 acgacgacgt gcttcaaaag tcatcttatt gactgtcatc ccatttccca ttgatgcgga
    96301 agggtcgcgt gtaaagtaca agtttggcat tgggtccatg taaaatggat agtcatcgtc
    96361 tgctgacaga tcatatagac tgctagaacg tacttcgata tcttttgtac gaaccccagc
    96421 catgatttta tctaccattt cttgcgtttc cattgataga aggaactcat gaagaccctc
    96481 tcttacagca ttcgatgcaa tatgagattc atccaacatt ttattcaaga attgttcttt
    96541 gacattgcct gcgtcaatcg cttctgccgc taatttttcc aagtaaagag tctcgacgcc
    96601 ttcattttgc aatgtcttcg caaagttgtc atgttcttct tgagcaatcg gaagatatgg
    96661 aatatcatcg aacaacagac gatccataat atctggtgtc aaattttcca cttcttgtcc
    96721 aggccgtttc agtaaaactg tctttaattt ccctatttca gagaaaacgt gaataggttt
    96781 atccatgttg tgttgcctcc ttctttttga ttacatgtat aggatatcct ccttttgtaa
    96841 tcactttcat aattttgtct cattcaaaat gagacattcc tcacattaat attcgataat
    96901 taaataatta aacgtttttc tatttttaag cgtaatttat tcatttttta ttctgtatac
    96961 actttcaaaa aaatatttat tcattgaaaa aatttttt
//
LOCUS       DAZUJO010000003        84960 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000003 DAZUJO010000000
VERSION     DAZUJO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.003"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..532
                     /locus_tag="ACG8OL_000182"
     CDS             14..532
                     /locus_tag="ACG8OL_000182"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4816200.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            660..1343
                     /locus_tag="ACG8OL_000183"
     CDS             660..1343
                     /locus_tag="ACG8OL_000183"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS4816201.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1652..1725
                     /locus_tag="ACG8OL_000184"
     tRNA            1652..1725
                     /locus_tag="ACG8OL_000184"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1686..1688,aa:Arg,seq:tct)
     gene            1884..2243
                     /locus_tag="ACG8OL_000185"
     CDS             1884..2243
                     /locus_tag="ACG8OL_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS4816202.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2366..3997
                     /locus_tag="ACG8OL_000186"
     CDS             2366..3997
                     /locus_tag="ACG8OL_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS4816203.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4059..4700)
                     /locus_tag="ACG8OL_000187"
     CDS             complement(4059..4700)
                     /locus_tag="ACG8OL_000187"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4816204.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4886..4962
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4982..5290
                     /gene="rplU"
                     /locus_tag="ACG8OL_000188"
     CDS             4982..5290
                     /gene="rplU"
                     /locus_tag="ACG8OL_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS4816205.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5317..5661
                     /locus_tag="ACG8OL_000189"
     CDS             5317..5661
                     /locus_tag="ACG8OL_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS4816206.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5674..5967
                     /gene="rpmA"
                     /locus_tag="ACG8OL_000190"
     CDS             5674..5967
                     /gene="rpmA"
                     /locus_tag="ACG8OL_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS4816207.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6401..9208
                     /locus_tag="ACG8OL_000191"
     CDS             6401..9208
                     /locus_tag="ACG8OL_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4816208.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9399..9890
                     /locus_tag="ACG8OL_000192"
     CDS             9399..9890
                     /locus_tag="ACG8OL_000192"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4816209.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9993..10982
                     /locus_tag="ACG8OL_000193"
     CDS             9993..10982
                     /locus_tag="ACG8OL_000193"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS4816210.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11015..11818
                     /locus_tag="ACG8OL_000194"
     CDS             11015..11818
                     /locus_tag="ACG8OL_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS4816211.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11839..12753
                     /locus_tag="ACG8OL_000195"
     CDS             11839..12753
                     /locus_tag="ACG8OL_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4816212.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12836..13210
                     /locus_tag="ACG8OL_000196"
     CDS             12836..13210
                     /locus_tag="ACG8OL_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS4816213.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13349..13594
                     /locus_tag="ACG8OL_000197"
     CDS             13349..13594
                     /locus_tag="ACG8OL_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4816214.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13766..14830
                     /locus_tag="ACG8OL_000198"
     CDS             13766..14830
                     /locus_tag="ACG8OL_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS4816215.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15076..15501
                     /locus_tag="ACG8OL_000199"
     CDS             15076..15501
                     /locus_tag="ACG8OL_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4816216.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15494..15943
                     /gene="nusB"
                     /locus_tag="ACG8OL_000200"
     CDS             15494..15943
                     /gene="nusB"
                     /locus_tag="ACG8OL_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS4816217.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16040..16894
                     /locus_tag="ACG8OL_000201"
     CDS             16040..16894
                     /locus_tag="ACG8OL_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS4816218.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16895..18232
                     /gene="xseA"
                     /locus_tag="ACG8OL_000202"
     CDS             16895..18232
                     /gene="xseA"
                     /locus_tag="ACG8OL_000202"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS4816219.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18237..18461
                     /locus_tag="ACG8OL_000203"
     CDS             18237..18461
                     /locus_tag="ACG8OL_000203"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS4816220.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18463..19353
                     /locus_tag="ACG8OL_000204"
     CDS             18463..19353
                     /locus_tag="ACG8OL_000204"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4816221.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19362..20180
                     /locus_tag="ACG8OL_000205"
     CDS             19362..20180
                     /locus_tag="ACG8OL_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS4816222.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20287..20736
                     /locus_tag="ACG8OL_000206"
     CDS             20287..20736
                     /locus_tag="ACG8OL_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4816223.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20754..22436
                     /gene="recN"
                     /locus_tag="ACG8OL_000207"
     CDS             20754..22436
                     /gene="recN"
                     /locus_tag="ACG8OL_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS4816224.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22491..22607)
                     /locus_tag="ACG8OL_000208"
     CDS             complement(22491..22607)
                     /locus_tag="ACG8OL_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS4816225.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22680..23636)
                     /locus_tag="ACG8OL_000209"
     CDS             complement(22680..23636)
                     /locus_tag="ACG8OL_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4816226.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23807..24175
                     /locus_tag="ACG8OL_000210"
     CDS             23807..24175
                     /locus_tag="ACG8OL_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS4816227.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24345..24776
                     /gene="mraZ"
                     /locus_tag="ACG8OL_000211"
     CDS             24345..24776
                     /gene="mraZ"
                     /locus_tag="ACG8OL_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS4816228.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24797..25756
                     /gene="rsmH"
                     /locus_tag="ACG8OL_000212"
     CDS             24797..25756
                     /gene="rsmH"
                     /locus_tag="ACG8OL_000212"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS4816229.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25761..26162
                     /gene="ftsL"
                     /locus_tag="ACG8OL_000213"
     CDS             25761..26162
                     /gene="ftsL"
                     /locus_tag="ACG8OL_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS4816230.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26163..28355
                     /locus_tag="ACG8OL_000214"
     CDS             26163..28355
                     /locus_tag="ACG8OL_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4816231.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28381..29343
                     /gene="mraY"
                     /locus_tag="ACG8OL_000215"
     CDS             28381..29343
                     /gene="mraY"
                     /locus_tag="ACG8OL_000215"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS4816232.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29357..30718
                     /gene="murD"
                     /locus_tag="ACG8OL_000216"
     CDS             29357..30718
                     /gene="murD"
                     /locus_tag="ACG8OL_000216"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS4816233.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30744..31832
                     /gene="murG"
                     /locus_tag="ACG8OL_000217"
     CDS             30744..31832
                     /gene="murG"
                     /locus_tag="ACG8OL_000217"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4816234.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31847..33067
                     /locus_tag="ACG8OL_000218"
     CDS             31847..33067
                     /locus_tag="ACG8OL_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS4816235.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33223..34548
                     /gene="ftsA"
                     /locus_tag="ACG8OL_000219"
     CDS             33223..34548
                     /gene="ftsA"
                     /locus_tag="ACG8OL_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS4816236.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34571..35812
                     /gene="ftsZ"
                     /locus_tag="ACG8OL_000220"
     CDS             34571..35812
                     /gene="ftsZ"
                     /locus_tag="ACG8OL_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS4816237.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35829..36506
                     /locus_tag="ACG8OL_000221"
     CDS             35829..36506
                     /locus_tag="ACG8OL_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS4816238.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36520..37119
                     /locus_tag="ACG8OL_000222"
     CDS             36520..37119
                     /locus_tag="ACG8OL_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS4816239.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37200..37421
                     /locus_tag="ACG8OL_000223"
     CDS             37200..37421
                     /locus_tag="ACG8OL_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS4816240.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37470..38252
                     /locus_tag="ACG8OL_000224"
     CDS             37470..38252
                     /locus_tag="ACG8OL_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS4816241.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38273..38980
                     /locus_tag="ACG8OL_000225"
     CDS             38273..38980
                     /locus_tag="ACG8OL_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS4816242.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39242..42040
                     /gene="ileS"
                     /locus_tag="ACG8OL_000226"
     CDS             39242..42040
                     /gene="ileS"
                     /locus_tag="ACG8OL_000226"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS4816243.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42089..43615)
                     /gene="zwf"
                     /locus_tag="ACG8OL_000227"
     CDS             complement(42089..43615)
                     /gene="zwf"
                     /locus_tag="ACG8OL_000227"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS4816244.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43630..44277)
                     /locus_tag="ACG8OL_000228"
     CDS             complement(43630..44277)
                     /locus_tag="ACG8OL_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4816245.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44461..44790)
                     /locus_tag="ACG8OL_000229"
     CDS             complement(44461..44790)
                     /locus_tag="ACG8OL_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS4816246.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44967..45695
                     /gene="sfsA"
                     /locus_tag="ACG8OL_000230"
     CDS             44967..45695
                     /gene="sfsA"
                     /locus_tag="ACG8OL_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS4816247.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45711..46724
                     /locus_tag="ACG8OL_000231"
     CDS             45711..46724
                     /locus_tag="ACG8OL_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4816248.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46724..48001
                     /locus_tag="ACG8OL_000232"
     CDS             46724..48001
                     /locus_tag="ACG8OL_000232"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4816249.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48064..50766
                     /locus_tag="ACG8OL_000233"
     CDS             48064..50766
                     /locus_tag="ACG8OL_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4816250.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50918..51235
                     /locus_tag="ACG8OL_000234"
     CDS             50918..51235
                     /locus_tag="ACG8OL_000234"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4816251.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51265..51585
                     /locus_tag="ACG8OL_000235"
     CDS             51265..51585
                     /locus_tag="ACG8OL_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4816252.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51693..53153
                     /locus_tag="ACG8OL_000236"
     CDS             51693..53153
                     /locus_tag="ACG8OL_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4816253.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53221..53442
                     /locus_tag="ACG8OL_000237"
     CDS             53221..53442
                     /locus_tag="ACG8OL_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816254.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53473..53655
                     /locus_tag="ACG8OL_000238"
     CDS             53473..53655
                     /locus_tag="ACG8OL_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS4816255.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53655..54068
                     /locus_tag="ACG8OL_000239"
     CDS             53655..54068
                     /locus_tag="ACG8OL_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS4816256.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54191..55372
                     /locus_tag="ACG8OL_000240"
     CDS             54191..55372
                     /locus_tag="ACG8OL_000240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4816257.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55443..55607)
                     /locus_tag="ACG8OL_000241"
     CDS             complement(55443..55607)
                     /locus_tag="ACG8OL_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816258.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55761..55834
                     /locus_tag="ACG8OL_000242"
     tRNA            55761..55834
                     /locus_tag="ACG8OL_000242"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55795..55797,aa:Arg,seq:cct)
     gene            complement(55903..57042)
                     /locus_tag="ACG8OL_000243"
     CDS             complement(55903..57042)
                     /locus_tag="ACG8OL_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4816259.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57214..57348
                     /locus_tag="ACG8OL_000244"
     CDS             57214..57348
                     /locus_tag="ACG8OL_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816260.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57341..57976)
                     /locus_tag="ACG8OL_000245"
     CDS             complement(57341..57976)
                     /locus_tag="ACG8OL_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816261.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58089..58724)
                     /locus_tag="ACG8OL_000246"
     CDS             complement(58089..58724)
                     /locus_tag="ACG8OL_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS4816262.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58758..59219)
                     /locus_tag="ACG8OL_000247"
     CDS             complement(58758..59219)
                     /locus_tag="ACG8OL_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFS4816263.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59349..59780)
                     /locus_tag="ACG8OL_000248"
     CDS             complement(59349..59780)
                     /locus_tag="ACG8OL_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4816264.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59798..60118)
                     /locus_tag="ACG8OL_000249"
     CDS             complement(59798..60118)
                     /locus_tag="ACG8OL_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4816265.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60417..61193
                     /locus_tag="ACG8OL_000250"
     CDS             60417..61193
                     /locus_tag="ACG8OL_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4816266.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61208..61411
                     /locus_tag="ACG8OL_000251"
     CDS             61208..61411
                     /locus_tag="ACG8OL_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816267.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61427..61765
                     /locus_tag="ACG8OL_000252"
     CDS             61427..61765
                     /locus_tag="ACG8OL_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS4816268.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61752..61931
                     /locus_tag="ACG8OL_000253"
     CDS             61752..61931
                     /locus_tag="ACG8OL_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816269.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(61974..62444)
                     /locus_tag="ACG8OL_000254"
     CDS             complement(61974..62444)
                     /locus_tag="ACG8OL_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816270.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62531..63229
                     /locus_tag="ACG8OL_000255"
     CDS             62531..63229
                     /locus_tag="ACG8OL_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS4816271.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63407..63748
                     /locus_tag="ACG8OL_000256"
     CDS             63407..63748
                     /locus_tag="ACG8OL_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816272.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63741..64412
                     /locus_tag="ACG8OL_000257"
     CDS             63741..64412
                     /locus_tag="ACG8OL_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4816273.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64418..65110
                     /locus_tag="ACG8OL_000258"
     CDS             64418..65110
                     /locus_tag="ACG8OL_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4816274.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            65107..65856
                     /locus_tag="ACG8OL_000259"
     CDS             65107..65856
                     /locus_tag="ACG8OL_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816275.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65868..66137
                     /locus_tag="ACG8OL_000260"
     CDS             65868..66137
                     /locus_tag="ACG8OL_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816276.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            66143..66307
                     /locus_tag="ACG8OL_000261"
     CDS             66143..66307
                     /locus_tag="ACG8OL_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816277.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66300..66599
                     /locus_tag="ACG8OL_000262"
     CDS             66300..66599
                     /locus_tag="ACG8OL_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816278.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            66599..66901
                     /locus_tag="ACG8OL_000263"
     CDS             66599..66901
                     /locus_tag="ACG8OL_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS4816279.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            66941..67306
                     /locus_tag="ACG8OL_000264"
     CDS             66941..67306
                     /locus_tag="ACG8OL_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816280.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            67796..67936
                     /locus_tag="ACG8OL_000265"
     CDS             67796..67936
                     /locus_tag="ACG8OL_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816281.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            67933..68115
                     /locus_tag="ACG8OL_000266"
     CDS             67933..68115
                     /locus_tag="ACG8OL_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816282.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            68128..68322
                     /locus_tag="ACG8OL_000267"
     CDS             68128..68322
                     /locus_tag="ACG8OL_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816283.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(68355..68567)
                     /locus_tag="ACG8OL_000268"
     CDS             complement(68355..68567)
                     /locus_tag="ACG8OL_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816284.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            68695..68895
                     /locus_tag="ACG8OL_000269"
     CDS             68695..68895
                     /locus_tag="ACG8OL_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816285.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            68892..69164
                     /locus_tag="ACG8OL_000270"
     CDS             68892..69164
                     /locus_tag="ACG8OL_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFS4816286.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            69165..69350
                     /locus_tag="ACG8OL_000271"
     CDS             69165..69350
                     /locus_tag="ACG8OL_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816287.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            69328..69684
                     /locus_tag="ACG8OL_000272"
     CDS             69328..69684
                     /locus_tag="ACG8OL_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816288.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            69644..70120
                     /locus_tag="ACG8OL_000273"
     CDS             69644..70120
                     /locus_tag="ACG8OL_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4816289.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(70266..70994)
                     /locus_tag="ACG8OL_000274"
     CDS             complement(70266..70994)
                     /locus_tag="ACG8OL_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFS4816290.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            71042..71320
                     /locus_tag="ACG8OL_000275"
     CDS             71042..71320
                     /locus_tag="ACG8OL_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816291.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            71322..71708
                     /locus_tag="ACG8OL_000276"
     CDS             71322..71708
                     /locus_tag="ACG8OL_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFS4816292.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            71705..72085
                     /locus_tag="ACG8OL_000277"
     CDS             71705..72085
                     /locus_tag="ACG8OL_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4816293.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            72197..72649
                     /locus_tag="ACG8OL_000278"
     CDS             72197..72649
                     /locus_tag="ACG8OL_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4816294.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            72646..74373
                     /locus_tag="ACG8OL_000279"
     CDS             72646..74373
                     /locus_tag="ACG8OL_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4816295.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            74437..75624
                     /locus_tag="ACG8OL_000280"
     CDS             74437..75624
                     /locus_tag="ACG8OL_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4816296.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            75596..76165
                     /locus_tag="ACG8OL_000281"
     CDS             75596..76165
                     /locus_tag="ACG8OL_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFS4816297.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            76178..77542
                     /locus_tag="ACG8OL_000282"
     CDS             76178..77542
                     /locus_tag="ACG8OL_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4816298.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            77544..77822
                     /locus_tag="ACG8OL_000283"
     CDS             77544..77822
                     /locus_tag="ACG8OL_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS4816299.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            77803..78129
                     /locus_tag="ACG8OL_000284"
     CDS             77803..78129
                     /locus_tag="ACG8OL_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4816300.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            78119..78457
                     /locus_tag="ACG8OL_000285"
     CDS             78119..78457
                     /locus_tag="ACG8OL_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816301.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            78447..78812
                     /locus_tag="ACG8OL_000286"
     CDS             78447..78812
                     /locus_tag="ACG8OL_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS4816302.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            78819..79445
                     /locus_tag="ACG8OL_000287"
     CDS             78819..79445
                     /locus_tag="ACG8OL_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4816303.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            79445..79909
                     /locus_tag="ACG8OL_000288"
     CDS             79445..79909
                     /locus_tag="ACG8OL_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816304.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            80104..82413
                     /locus_tag="ACG8OL_000289"
     CDS             80104..82413
                     /locus_tag="ACG8OL_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4816305.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            82425..83129
                     /locus_tag="ACG8OL_000290"
     CDS             82425..83129
                     /locus_tag="ACG8OL_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4816306.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            83126..>84960
                     /locus_tag="ACG8OL_000291"
     CDS             83126..>84960
                     /locus_tag="ACG8OL_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4816307.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        28620 a        13992 c        17856 g        24492 t
ORIGIN      
        1 gaagttgaaa acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat
       61 tatattttta attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc
      121 ttatattatt gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt
      181 acgaccgaaa aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc
      241 aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta
      301 tacaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat
      361 cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc
      421 atggattcag gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa
      481 tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta
      541 aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca
      601 ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta
      661 tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta
      721 cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag
      781 aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc
      841 agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca
      901 cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga
      961 aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc
     1021 aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg
     1081 caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg
     1141 ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc
     1201 cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc
     1261 atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg
     1321 aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat
     1381 cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata
     1441 caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat
     1501 aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc
     1561 aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt
     1621 atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc
     1681 cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag
     1741 aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag
     1801 gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac
     1861 tcatcagaaa agaggaagaa gatatgacga tactatctag aattttagtg actctagttg
     1921 caattgaatt tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa
     1981 gtagggtttt taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta
     2041 agaaccaagg agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt
     2101 cttccgcatc taaagaaatc acaagtatgc tattaatatc tatttttttt gttgcattat
     2161 atggcagttt gactagcgat cgttggattt tactcaaaca aggtgggcta gctctgttgg
     2221 cattgctttc tattttctta taaatataga aaaaataaga atttatctaa caggaaactt
     2281 tgtgaaaaat attactttat ctcatgaaat atgatattct aatgaatggt attttagaaa
     2341 atttcatgaa aaaggagtta gataggtgtt caaaaattat gtttcctcac taaaaaaaga
     2401 attttcaggt tatgatcttt caagatttca gaaagatctt ttggctggga tcacagtagc
     2461 tgctgtagca ttgcctttag ccttggcttt tggggtatca agcggagctg atgcggccgc
     2521 aggtttgatt acggctatca ttgccggtat cgttattggc ggtttatcag gcggttttta
     2581 tcaaatatca ggtccgacag gtgcgatggc agctatcttg atgtctattg cggcaacaaa
     2641 gggaatgcaa ggtgtattgc ttgctacttt tttagcgggt ctatttttac ttgcagcagg
     2701 acttttccga ttaggaacac tgacttcttt tatcccagca cccgtaatta cggggtttac
     2761 ttcaggtatt gctatcatta ttgcattagg tcagatcgat aatttatttg gtgttcactc
     2821 ggaaggtaca aacgttattg aaaaactaag tagttatcaa acgataggat ttccgataaa
     2881 cacagcctct ttgatcatgg gagggctagt cattttaggg atgtttatct atccaaaaaa
     2941 atgggcgaaa cgaatgccta gttcgctgct ggctatcatt ttggcaacag cagttatgct
     3001 attgttccat ttaccagtag caactgtggg taagattcca caaacattga tcagtagtaa
     3061 tcgtttgaat atgggagatt ttagcttttc tgccttgcaa caagtggcag ttccagctat
     3121 tagcattgct ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg ctggccgaat
     3181 ggcaaataag cccttagata gcaatcaaga attaatggcg caaggaattg gaaatctgtt
     3241 gcttcctttc tttggcggga ttcctgctac tgcggccatc gcccgtacaa gcgtggccat
     3301 caaatctggt gcacaaacta gattagctgg tatgatacat gcagttgtct tactgctctc
     3361 catgcttgtt tttgcgccag tgatgtcaca gattccaatg ccagcacttg caggtgtttt
     3421 gattgtgacg gcttggcgta tgaatgaatg ggaaacgatc aaggagctat ttactaataa
     3481 atattggtca gcagttctat tgtttatcct tacgatgatt tgtacagtta tctttgattt
     3541 aagtattgct atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg tcaaaagcgc
     3601 tgctatcacg atttcattag aaacgatcga ttggcaaaga atggggctgc cagaaacgga
     3661 aaagctgaat aactggacag tagtgtatat tagtggtcca ctgtttttta tgtcttcaga
     3721 aaaactcaaa aataaattgg aagaactaaa tcatcacgat ggtgtcattt tctccatgcg
     3781 aggcgtacca agtattgatt tgacggcacg tgcagttttt gatgaatttc aagaaaaagc
     3841 agagcaaaga ggacaaacga ttatctatac ttccttgcag ccagaagtag aaaaacaact
     3901 ttctcacttg tgggaacata cattcacgca tcaacatcaa acagttgctc atgcgttgac
     3961 agctttacat gcgaaatata ctgcaattag ccattaaaac aatgaaaaag aacagacaag
     4021 actgtaaact gaagatttta tggtcttgtc tgttcttttt aatattgctg taaaagtgaa
     4081 gctagtactt gccagtcttg atggataaca ggtacaaggg agcgattgta gaaaatagct
     4141 gctggatgaa aaagtggaag aattcgatag tttttttctg accattcgta ggcatccttt
     4201 tgttctgtta attgtaagac aggccctgta tagagttgac catgattttt ggtaaccgta
     4261 tattctttgc caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat taattttggc
     4321 tggaccttca tcagttcgta atccaatatt ggtgcatgcg caaatacttc ggaacgagta
     4381 ggtgtccgat ttggataaac agtttctgac tgttgtgtcc gtttattgat ccgatgagtc
     4441 acgcgatacg ggcggctcct tacagcactg gtgatataca cctcttctcg tgttaagcca
     4501 gttgaagaaa gtgcctgcat caattctttt ccagcttgac cgctaaaagg aacatgcgta
     4561 tcaatctctg ttttccctgg ggcttctccc acgagcatca gtttaggatg aagaggtcct
     4621 tgcccagcga taaatccttc tacaggacgg ccgttcattc ttaaagaagc ttcttgtatc
     4681 agttcttttg ggtattccat ttatgctcct cctttttctt ttattataag aataaaagaa
     4741 acacaatgcg actattttat ttggtagaac tgtgaggata gaaaagcaga agtattgctt
     4801 gaatttttgt tttacttttt gattttaaat ttagttgact aacggttgct gttctggtaa
     4861 gctagtactg ttgtaattgt ggactccaca gctacaaccg ctcagaacaa gtaataagca
     4921 cagcatatgc tgtcacttcg gatggcgagt ctaagtatat cagaaggagg tgctttatag
     4981 catgtacgca attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg gacaagcaat
     5041 ctatgttgaa aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg aagttatctt
     5101 agtaggcgga gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa ctgttgaagg
     5161 tactgttgaa aaacacggca aacaaaagaa agtcgttact ttcaaataca aacctaaaaa
     5221 acattcacac cgtaaacaag gtcatcgtca accatataca aaagtggtta tcgacgcgat
     5281 caacgcttaa tagcgtttga gaaagaaggc ctatatatga tcaaaggaac atttaagcga
     5341 aatgacgcag gtcagatcgt ttcattcact ttgaccgggc atgctgatgc tggtccttat
     5401 ggaagtgata tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt caacgggatt
     5461 gcctcattag ccggttttga gccgattgtt gaaatgaatg aagaagaagg cggttatctt
     5521 tataccgaag tcacttcagg aatgacacaa gaacaaaaca atattgctga gattctatta
     5581 gaaaatcttt tattaggatt acaatcgata gaagctgaaa actcagaata tatccaaatt
     5641 aaaacaatta atgataaata ggaggtgcag accatgttgt tatctatgaa tctacaatta
     5701 tttgcccaca aaaaaggtgg cggttctaca tctaacggcc gtgactcaga atcaaaacgt
     5761 ctaggtgcta aacgtgctga tggacaaaca gttacaggtg gatcaattct ttaccgtcaa
     5821 cgcggtacaa aaatctatcc aggtgtaaac gtgggtatcg gtggagacga cactttattt
     5881 gcaaaagttg atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa acaagtgtct
     5941 gtttatccag tagctcaaga agcttaattg aatcttatga ttaaagaggt tgtgacaaga
     6001 cttttgtcac agcctttttt gtttataaaa ataaaagttg tagtggaggc tacgaagttt
     6061 actttgtctt tttacactta atattttttt gccaatttgg aatcaggatg ttttgataac
     6121 tatcgttttc aattgatgga agctatattg tttgctatca gtgaaataat tatatggtaa
     6181 acagtgttct ttttcttgtt tccattataa aaatcgtatc gggatttttc gttcttttaa
     6241 attgaacatt tctatctatc cgcgtgttat caacaaaaca aaatcaggta aatagttgac
     6301 gaaacagaaa taaatacata gtattatgtt ttgaagccta agtacaatag tctatagaaa
     6361 aggagcgata ccattggcaa aacgcattga ccgaatttat atgtatgtca aagaaaaaac
     6421 cgctcattta acaaaggcag aatatgatca aggagtaaca acacaggaaa ttgctgaagt
     6481 gttagggatt cagcgaacaa atagcagtaa agatctgaat cagctagtgc gcgaaggaaa
     6541 actgataaag acagacggac gaccagtacg ttacatatat caaacactcg ttacgcacgt
     6601 aaagccagtt acaaaacatg tagtcagtta caaagaaacg ccggtctctg aagaaaaaaa
     6661 tacactccaa atagatacta aggatatctt tgcaaaaatc attggagcta acggcagcat
     6721 gaaaaattca gtggaacagg caaaagctgc gattttgtat ccgccaagag gattaaattg
     6781 tttgattaca ggacctaccg gatcaggaaa gacttatttt gcacacgcga tgtatcattt
     6841 tgcaaaagcg catcatgtaa tagatgagga aacggaactg attgttttca attgtgctga
     6901 ttatgccaat aaccctgagc ttttgatgag tcatttattt gggtacgcaa aaggtgcttt
     6961 tacaggcgca gatgaagaaa aaatggggat tatcgatcaa gcggatggcg gtatgctgtt
     7021 tttggatgag attcaccggt tgccgcctga aggtcaagaa atgatctttt actttatgga
     7081 tcatggaact tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa atgtacggat
     7141 cgtaggtgcg accacagaag acccaggttc ttctctatta gaaacttttg ttcggcgtat
     7201 cccaatcaat atcaaacttc cggcttttga aaaacgtcct gcaaatgaga aagtcgattt
     7261 agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag cgaaagattt ctttgacaga
     7321 agatgtagca aaagcattga tcggcagtgt gacctatgga aatataggac aattgaagtc
     7381 aaatatccag ttagtatgtg cacgaggatt tctaaatcat atgcaatcac ccgaaatctc
     7441 gatcacaatc gatgatttga cagaagggat tcgtagcgga ttgattcaat tagcaagcaa
     7501 ccgcagtgca atggcagagc tttctaaaat attggagcca aagataactg tagcaccaaa
     7561 tgatacgatc atgaaaatcc aatcggactc ctatgagtta ccttataatc tatatgatat
     7621 catcggagat aaagcagctt tactgaagtc ggatggtttg gatcaagaag caatcaatca
     7681 tttcatttct acagatatca acatccatct gaagtctttc tataaagacc atggtttttc
     7741 ttttaatgca gacagcaaat tagcagaatt tgtagatccg aaaatcatag aagtgaccaa
     7801 tcagatttat gtaatggtca aaagatcttt accatatgag tttcagcaaa actttattta
     7861 tgctatgagc ttgcatatca gttctttttt gaaaaaaatc aacttaggag aagaacggca
     7921 tacgaatgat aacatccgtg agatggcttt tgattttcca gatgaatatg ctgttgcgaa
     7981 agaagtgcgt agttatatcg aaacgtattt tcaagtggag attccagaaa gtgaagacta
     8041 ttacttgacc gttctactcg tctcgttacg ggcaaatcaa gcaagcggca gaatcggtgt
     8101 agtcgttgca gctcacggaa acagcacagc aagcagtatg gtccaagttg tccaacagtt
     8161 gttagatgca gagaacgtac gagcagtaga catgcctttg gatatggatc cgaaaacagc
     8221 attggaccga attgaacgca atgtacaaga agtagatgaa ggaagcgggg tcatactctt
     8281 agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca ctggaattcc
     8341 agtacgtact gttgatatgg taacaacttc tctagttttg gaaactgtac gaaaggtctc
     8401 agtattaggg acagatctag atatgttgca tgaatcattg aaaaattttc gaggttacgc
     8461 agaaatatct tctgtagaat ctgttcagga agcaaaacct attcataaga aaaaagctgt
     8521 tttggctatc tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat tgatcgaacg
     8581 agcggtaaac aagcgtcaag aaacagaatt agaagttcta gctttgtcga ttgtggaagt
     8641 gaaagaagaa ctgccagtta tccaaaaaga atatcagatc atagctacta ctgggattac
     8701 tgatttgaat atccaagccc catttattcc tttagaacgt tttattgatc aaaatatcga
     8761 agtcattttg gatcagttgc tgatggaatc agaattggag gaaactgaat tcatctccct
     8821 agatgaagag tcagctaaaa atacgtgtgt tgagtttatc tctgataact tcatctttat
     8881 taatggtagt aagttaatcg atccaatgtg gcaattttct acacaaatct ctcagacgac
     8941 gggaatcggc gatgaagaat atggattcaa aatcaaccta gtgatgcaca cagcaggaat
     9001 gatcgaacgt attattcgga atgaaccatt gactgtggaa gaaaacgaat tgacaaatac
     9061 aacgaatgat cctctttata gccaattggc agcctcagtt gttctgttgg aagatcagat
     9121 aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta cgtctagtcc ataatcagtt
     9181 agataaaaaa gaatacactg tgccatgaat acacaaaaac gatacacttt tatagtgtat
     9241 cgtttttgtg tattggttaa gaatccttta aattcaatta gtgtatcgta aaaaagttgg
     9301 cacgcttttt gcttatagta gtgtgtgcta ggaggtggtt gttcacacag aaatgatagg
     9361 ggtttctgtg aagtagtgag gaagaaggag ttttgcagat ggatattcga ttggcacgta
     9421 tagatgatcg tttaatccac gggcaagtcg caacagtttg gtcgaagcaa acaggtgttg
     9481 aacgtatcat tgtgatcagc gatgtagtcg ctcaagataa attgagaaag tttctactaa
     9541 aagaagctgc accgccgggg atcacagcaa atgtaataac cgtgcagaaa atgattcaaa
     9601 tctatcatag tggattatta tcacgagcaa aagtgatgct tttattcacc aatccccaag
     9661 atgttgaagc ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc ggcggtatga
     9721 gttttactga aggcaaatca atgattacca attttgtttc agtaaatcaa caagatattg
     9781 attcctttcg ttacttgaac agtcaagaag tggagttaga gattcgaaaa gtacctgctg
     9841 acaggaaaat ccttttgatg gaactactag cgaaaatcaa aaaggtgtag caagaatggt
     9901 tgttggaaaa ggtcaaataa ttctgttgta atggttagca attattaata tttttgaatt
     9961 tgttacaaca aaagtaaata ggaggtataa atatggtagg gattatccta gcaagtcatg
    10021 gacaatttgc tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa caagaaaacg
    10081 tcaaagccgt gattttgaaa ccaagtgaag gtccagatga cttgagagcc aaattggaag
    10141 aagcggttgc ttcttttgat aatcaagacg aagtgttatt cttagttgat ttatggggcg
    10201 gaacaccgtt caatcaatca aacacattat tcgaagagca caaagataaa tgggcaatcg
    10261 tcagcggttt gaatttacca atgttgattg aagcctacgc atcacgcttt tcaatggaat
    10321 ctgctcatga aattgcagca cacatcattg aaacagcaaa agacggtgta aaagtaaaac
    10381 cggaagaatt agaaccggca gaagcaccga aagcagcagt tgaagacgca caacctaaag
    10441 gagcacttcc agaaggaact gtggtcggag acggaaaaat caaatatgta ttagcacgtg
    10501 ttgactcacg tctgttacat ggacaagttg cgacagcttg gacaaaagca gtgcaaccaa
    10561 accgtattat cgttgtttca gatgcagtat caaaggatga tctgcgtaag agactaatcg
    10621 aacaagctgc tcctccaggt gtaaaagcaa atgttattcc tattagcaaa atgatcgaag
    10681 tagcaaaaga ccctcgtttt ggaaatacca aagcattgct tttatttgaa aatcctgaag
    10741 acgtattgac agctgttgaa ggtggcgtag acatcaaaga attaaatgtt gggtcaatgg
    10801 cgcattcggt cggtaaagtc gttgtaagta aagtattgtc tatgggacaa gaagacgttg
    10861 aagcgtttga gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa gtacctaatg
    10921 attcacgcga taacatggat gatattttga aaaaagcaaa agctgaatta gcaaaagcgt
    10981 aataaataat attaaaattt ataggaggct tatcatgtct attatttcaa taattttagt
    11041 cgtattagtt gcctttctag ctggtatgga aggtattctt gatgaatttc aatttcatca
    11101 accactagtg gcatgtacat tgatcggttt agttaccggt aacctcgaag ctggtatcgt
    11161 acttggcggt acgcttcaga tgatcgcttt aggttgggca aatatcggtg ccgctgtagc
    11221 accagatgct gcattagcat cagtagcatc agcaattatt ttagttttag gtggacaagg
    11281 tgttaaaggt gttccttctg cgatcgcgat tgccgttcct ttggctgttg cagggttgtt
    11341 cttaacaatg atcgtacgta caatcgctgt tccaatcgtt catttgatgg atgcggctgc
    11401 tgaagaagga aacatccgta aggttgaaat gtggcacatc attgctgtat gtttacaagg
    11461 ggttcgtatc gcggtcccag cagcagcgtt gctatttatc ccagcacaaa ctgtgcaatc
    11521 attccttgaa tcaatgccag catggttaac agatggtatg gctatcggtg ggggaatggt
    11581 cgtagctgta ggttacgctt tggtaatcaa catgatggct acaaaagaag tatggccatt
    11641 cttcgttatt ggtttcgttg tagctgcaat ttctcaatta acattgattg ctctaggtgc
    11701 tttaggtgtt gcattagctc ttatttactt gaacctttct aaaatgggtg gttcatcaaa
    11761 taatggaggc ggaagcaatt ctggtgaccc tttaggcgac attttgaatg actattaatc
    11821 ttgaaggagg aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa agatcgttta
    11881 gctgttgcgt ggcgctctac cttcattcaa ggttcatgga actacgaacg tatgcaaaac
    11941 ggtggctggg cattttctat gattccagca atcaaaaaat tatacaaaac aaaagaagaa
    12001 cgttctgcag cattaaaacg tcacttggaa ttctttaata cacacccata cattgcttct
    12061 ccaattttag gggttacatt ggctctggaa gaagaacgtg cgaatggcgc gcctgttgat
    12121 gatgtagcga ttcaaggggt taaagtaggt atgatgggtc ctttagctgg tgttggtgac
    12181 ccagtgttct ggtttacagt acgtccaatg ctaggtgcac taggtgcttc attagctatg
    12241 ggaggaaata tccttggacc aatcatcttc ttcttaggtt ggaacattat tcgttgggca
    12301 ttcatgtggt atacacaaga atttggctac aaagctggtt ctaaaattac tgatgacctt
    12361 tctggcggat tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat gttcgtatta
    12421 gctgcccttg tacaaagatg ggtatcaatc aaattcttac cagttgtatc aactgtaaaa
    12481 ttagataaag gtgcttacat cgaatgggat aaattgcctt caggtggtga aggaatcaag
    12541 agtgcatttg aacaagttaa taatggctta gccctttctc ctgaaaaggt aacaacatta
    12601 caagacaact tagaccaatt gattcctggt ttagctgcat taggattaac attgttatgt
    12661 atgtggttgc ttaagaaaaa ggtttctcca atcgttatca tccttggttt attcgtagtc
    12721 ggtgttgttg gacacgtcat cggactttta taatccataa aaatatagct cgggcgttgt
    12781 cccgagcttt ttttatatag tatactgttc ataaacaaat atctggaggg agaaaatggt
    12841 tcaatcaatc aatacaaaag tagacttggt aattgatgct acttcgttta cgggtctgac
    12901 agattacggg caaatcatga taggtaataa tggttttgag ttttaccatg cgcgagatag
    12961 acgcaaatat atccaaattc catgggaaga agtggattat gtactagcat cagtcatgct
    13021 aaagggaaaa tggattcctc gatatgccat tcaaacgaaa aaaaatggta catacacatt
    13081 ttcttccaaa gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag atgaagatag
    13141 aatggtacaa tcattgagtt tctttgatgt agtgaaacgt ggtattcgta gtatgtttaa
    13201 acgcaagtaa aaaataaaga aaacttctaa gaggaggttt tctttatttt tttactttgc
    13261 agaagaaaat atgaaatagc aaaaccaatt atttgtcccc tttaaatccc atatgctaaa
    13321 attgattagc aaagaaggag gttatattat gagttggtta tggacattga ttgttggagc
    13381 cgtgatcgga gcaattgcag gtgcgatcac cagtaaagga aattcgatgg gctgtgtaac
    13441 gaatatcatt gctggactag tcggttcaag tattggacaa gcgattttcg gcgaatgggg
    13501 accaaaagct gcaggtatgg cacttattcc atctatttta ggcgcagtga ttttggtagc
    13561 agttgtctca tttttctttg gaaaacgatc ataagttttt acaaaatagt aaactaaaag
    13621 gagttgcagc gagactgtga ctcctttttg gctttattta tttaaaataa aaagaagttt
    13681 tccggaaatt tttatttaac tgtcgtgtac gaagaaaagt ttgctataat ggaaaaaagc
    13741 gtgcaaagaa agagggagtt tcattatgat gctacgtgta gaaaaattgc gcaaaaaaat
    13801 gcaagaagaa aatttggatt catttttggt taccagtccg tacaatcttc gttacttaac
    13861 gaactttaca ggtacgacag gattagctgt tattactttg gaaaaagcat tttttattac
    13921 tgattttcgt tataccgagc aggcggcagc gcaggctcaa gggtttgaaa ttattaaaaa
    13981 tgtcggacct atttttgatg aggtagcaaa cttagtacgc aaagaagaac tgcactcatt
    14041 agcttttgag gaaacaacag tctcattttt agaatattcg gttttagaag aaatcatcga
    14101 tgcggaatta gtacctgttt cacagatgat cgaagaactc agagaagtga aagatgaaga
    14161 agaaatcgct attatcgaaa aagcatgtca tattgcggat atggcatatg atcacatcct
    14221 gaaaatgatc caaccgggca tgactgagat tgaagtcgca aatcaactag acttttatat
    14281 gcgttctctt ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg gattgcgttc
    14341 ggctatgccg catggtgtag caagcaaaaa aatcatcgaa caaggtgact tgatcacatt
    14401 ggactttggt tgctattacg aagggtacgt ttccgatatg acacgtacat tcgctattgg
    14461 tgatcctggg gaaaaattaa aagaaattta tcaaatagtc ttagatgctc aattagcagt
    14521 tattgatgct gcaaaaccag gaatgactgg tgtccaatta gatgcagttg ctagagatta
    14581 tatttcaaaa catggttacg gggaagcatt tggacattct acaggacatg ggatcggttt
    14641 agagatccac gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg taccaggaaa
    14701 tgtgatcact gatgaaccag ggatctactt accaggaatc ggtggagtac gtattgagga
    14761 tgacttgttg atcacagaag atggaaatcg tgtgttgact cgttcaccaa aagaattgat
    14821 catcttatag tttcatcaat agaagaagta agcgtaaaaa tcagtgttca cagactttgt
    14881 tatagaaaat cactcgattt aatgatacac tatagaagaa cttttaagct gtaagagata
    14941 ataaagttgg gtgttttctc caattcatag cagtttgcag ctgatcggcg gcagttttct
    15001 taccacgtga tcactatgcc gcatcataga gaattcggat gtatctcgtg cttaggcaaa
    15061 gggaggaata aataaatggc agatgaaaaa aatttgattt taaacgcaaa ccaagattta
    15121 ggagagatcg tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc agcttctaaa
    15181 gtagaaggcg tatacggaat gcgcggaact tttgccagca atgtcactga attattagga
    15241 cgtgcagctc acggaaaagg tgtttactta gcaaacgagg aagatggctt gaaagtagat
    15301 ctttactgtt atctagaata tggtgtttca gttcctaaag tagcaatgga aatgcaggaa
    15361 cgggtcagcc agcaagtcct tttcatgacg gatatcgatt tatctgaagt caatatccat
    15421 gtagtagctg ttgttcctga aaaattagaa caaccaacaa tcgaagaact ttttgaagac
    15481 gaagaggaag agaatgaata aagaattatc aagacacgag attcgggaaa tggcattgca
    15541 ggctttattt ccattagact tcaatgcaga cctaacaaaa gaagatgcga ttttcaacgc
    15601 aatcgaatta gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg ttcctgttta
    15661 tcttgatacg ctagttggcg gtgtctgtgc aaaaaaagat gaattggata aagtgattga
    15721 aaaacattta aaaaacaatt ggcacatcaa tcggatctca aaaatggatt tggtgatctt
    15781 gcgtatcgcg atttttgaaa tgacttatgt gacagatgta ccagcggctg ttgctttaaa
    15841 tgaagcaatc gagttggcaa aaacatttag tgatgaccgt tcacgtaaat tcgtcaacgg
    15901 tgttttatcc aatgtattga aagaattaga agctggggcg taagtcctag cttttttcta
    15961 tgaatcatca taaacctgtt ataaatcggg aaaattctat ggtagaatga aaacacaaaa
    16021 aatatctggg agtggttaga tggcagaatt gattaacgga aaagaattag ctgaaaaaat
    16081 gcaggcagag attgctgaaa aaatccaaaa attgaaagat aatggtatcc atcccggatt
    16141 agtcgtttta ttggtagggg aaaatccagc cagccaaatc tatgtaagaa ataaagaacg
    16201 tgtagcaaaa gaaatcggaa tccattcatt ggtagagcgt tatcctgaga cgatttctga
    16261 agacgaacta cttgcagaaa ttgaaaaata caatcaagat cctagttttc atggtatatt
    16321 ggtccaatta ccattgccaa agcatataga tgaagaaaag atcctgttag ctatccgccc
    16381 tgaaaaagac gtagacggtt ttcatccgat gaatttaggc agactccttg cgggtaaacc
    16441 ggacaagatt ccttgtactc catacggcat catgaaaatg tttgaagctt attctatcga
    16501 tccggcgggt aaacgagcag tagttatcgg acgaagcaat atcgtcggta aaccgatggc
    16561 tcagctactc ttgatggcag atgcaactgt tactgttgct cattctaaaa ccgaaaatct
    16621 ggctgaactg gcacaagaag cagatatttt agtcgtagca ataggacgtg gacattttgt
    16681 cacaaaagaa ttcgtaaaac caggtgctgt agtgatagat gtagggatga atcgtgatgc
    16741 taacggtaag ctgatcggtg atgtgaaatt tgatgaagtg gagcctattg caggctggat
    16801 cactcctgtg ccaaagggtg tagggccaat gactatcacg atgctgatgt atcaaaccgt
    16861 aaaatctgcc gaagcttttg gagcaggtga atagatggca caagaatatt taactgtcac
    16921 tacattaacg aagtatttga aaagaaaatt tgatgccgat ccttatttag aacgcgtata
    16981 tttaacaggc gaaatatcga atttccgttt acgtgccaat gcccatcaat atttcagctt
    17041 aaaagatgac catgcaaaaa tatctgctat tatgttcaaa ggagcattcc aaaaattacg
    17101 attccagcca aaagaaggaa tgaaagtcct tgtcgttggg cgtatttcat tatatgaagc
    17161 gggaggttct tatcaaatct atgtagagca catggagcct gatggaatag gtgctttata
    17221 tcaagaattg gaagaacgaa aagaaaagct gatgaaggaa ggcctttttc aagggcctaa
    17281 aaaaatgctg ccacgtttcc caaagcgaat cgcggtcctt accagtccaa gcggagcggt
    17341 tatccgcgat atcatcacga cggtgaaaag aagatatcca attgtccaat tggttttgtt
    17401 cccaacagtt gtacaaggaa accaagcagc ggacaatgtt gtcaggaata tccagagagt
    17461 agaagagatg ggcaatttcg atacaatgat catcggtcgt gggggtggtt cgattgagga
    17521 tttatggccg tttaatgaag agcgggttgc tcgggcgatt tatcaagcta atacgccggt
    17581 gatttcatct gtaggacatg aaacagatac cacgatcgct gatttagtag cagatgttcg
    17641 ggctgccact ccaacagcag ctgcagaatt agctgttcct gttttgtcag aagagttgat
    17701 gcgcattcaa gaacggcaag ctagattgca gcaggcgttt gtacgccaga tccagcgcaa
    17761 acaagaacgg ttcgagcgtg ctcgacgttc ctatgtattt cggcagcctg aaagattgta
    17821 tgaaggtcag accatcaaat tagaccagtt gaaacagcga ttatatcaag ctacacagca
    17881 aatttatcat gcgaaagaaa aacaaacgat cgcattaagc caccaattgg aacaagtggc
    17941 tccgctttat cgagttaaag cagctcgcca agaaactgct tatctagaaa aacgcttaga
    18001 agagaaaatc caacaatata tgaaacaaca gcagcagcgt tttgctcaag ctgttcaatc
    18061 tcttgatttg ttgagtccat tgaaaatcat gggaagaggc tacagctata cgacgttaaa
    18121 tgatcaagta gtaaaaagtg tcaccgagat tcatcaaggg gatttgctga cgatccatta
    18181 tcaagatgga atcgttaaag gacaagtaca agagatagac aaaggagaat aaaaatatgg
    18241 aaaatccaac ttttgaagag tcactgcaag aattagaaaa aattgtcatg cagttagaac
    18301 aaggagacgt tccattagaa tcagctctag attcgtttaa acgaggaatg gaattaagca
    18361 aacattgcca agatacatta gctaaagcag aagagacctt gacgaaaatg atgacagaat
    18421 caaatgaaga agttgatttt gacggaaacg aggaaagctg agatgagatt aactgacttt
    18481 tcagctattc atctacctgc tgtagaaaaa gaaatcttgt cttttttaga tgagcataca
    18541 acggatagag gactctatga tgcgatgagt tattcagtga aagcaggcgg gaaaagaata
    18601 cgtcccttat tacttttaac ggccgttgct tcctttgatg agcctattga tgttcccgtc
    18661 tatcaagtag cagctgcttt agaaatggtg catacgtatt ccttgatcca cgatgatttg
    18721 cctgcgatgg acaatgatga tcttcgtaga ggaaaaccta ccaatcataa agtattcggc
    18781 gaagcattag ctatattggc cggagatggt ttgcttacag gagcattcca gctaatcagt
    18841 atggcacact taggtaattc tcctaagctt ctgctgcttc agcaattagc tgtttgtgcg
    18901 ggatcacaag gtatggttgc tgggcaagct gcagatatcg agggcgaatc gaaaaagctt
    18961 tctttagaag aattagcatt tattcatgaa agaaagacag ggcatttgat tcgttatgcc
    19021 ttgctggctg gtggcatatt agcgaagcaa cctgaagaaa tcttattgct acttcaacga
    19081 ttagccgaac atttaggttt agcttttcag ataagagatg atctactcga tgtgattggt
    19141 acaacgaaaa cattagggaa aacagctggt aaagatgaac gtatggaaaa aaatacttac
    19201 ccgcgtttat taggcctaga aaagacacgt gaggcgttgg agattgagct gttatccgcc
    19261 aacaagatca tagacaaatt agaagaggac gttttttctt ttgatggcgg gctgatgcgc
    19321 cagatgatca agcaatttga tgtggagaaa tgagggaaag aatggaaaaa gaacgcgtag
    19381 atgtattagc ggtcaaacaa ggattatttg aaacaagaga acaagccaaa cgttcagtga
    19441 tggctggttt gatttacaac gaaaaaaacg agcggtttga taaaccggga gaaaaaattc
    19501 cagtgagtag tgaattaaga gtcaaaggta aaaaacttcc ttatgtttct cgagggggat
    19561 taaaattaga aaaagcactc aaagcatttg atctatctgt tgaaggaaaa acattgttag
    19621 atattggtgc atccactggc ggatttactg atgcagcttt gcaaaatggc gcaaaaatga
    19681 gttatgcttt agacgttggc tacaatcagc tagcttggaa aataagacaa gatcctagag
    19741 tcgtcgtaat ggaaagagtg aattttcgtt atgcaaaacc cgaagacttt accttaggaa
    19801 ttccagaagt tgctgttatc gatgtttctt ttatttcact aaaactgatg ctgcctccat
    19861 tacatgctat tttaaaagaa gagggagaag tgattgcttt aatcaaaccc caattcgaag
    19921 caggcagaga agctgtaggg aaaaacggaa ttgttcgtga ccctcagacc catcaaaaag
    19981 ttttagaaga cattttgtca tttgcggcac aaggtggata tgatgtgctg gaattaagtt
    20041 attctccgat cacgggggga gaaggaaata tcgagttctt ggctcacttg aggaaagtac
    20101 ccgaaagcgg aacaatcaat tcagctatta atatggcaga agtggtttca aatgcacacg
    20161 aacagtttga ccataaataa attgtaactg tgaaaatcaa gtgagaagta tgcggattct
    20221 tctttattat accaaggaaa taagctatca ttgattaaga agaatatgct aaggagcgaa
    20281 gacgtaatga gaaaaaaaga tcggcatcgt ttgattactc gattgttgag tgagcaagat
    20341 atccgaaagc aggaagaatt tgttgaaatc ctgaaaagca aaggaatatc tgttactcaa
    20401 gctacgattt ccagagatat aaaggaactg aaactgatca aggtaccggc ttcagatggc
    20461 ggctaccgtt atagcatacc agctgaaaca aacgaagatg taaatactaa attggaaaaa
    20521 ctattaaaag acgctttcgt ggcagttgac caaatggaaa aatttgttat tttgaaaaca
    20581 ttgccgggaa atgcttcggc agcagcaaat ttgattgata aacgttacaa gaaagaatta
    20641 ttttcgatta tcaatgatga cgataatgtt ttgatgatta cacgaactga agaccaggca
    20701 gttttattga agaaagactt cctgcattac ctttaagttg aggagcgaat gtgatgctac
    20761 aagaaatcag tattactaac tttgcgatta ttccggaatt gcggctttct tttcacgaag
    20821 gaatgaccgc gctgaccgga gagactggtg cgggtaaatc cattattatc gatgctttag
    20881 gtctgttagc aggaggaaga ggttcaagcg attatatacg tcaaggagca gaaaaatgtg
    20941 tgcttgaagg gttgtttgaa ctaccaaaac aagaaggatt ctctgaactg atggttgaat
    21001 tagggatcga aacagatgag gacaatctga ttgttcgacg ggatatgtcc ttaactggaa
    21061 aaaatgtttg tcgagtgaat ggtcatatta tcacattagc taatctgaga agaatcggca
    21121 gttatttagt agatatccaa ggacaaaacg aacatcagga actgctccag cctgaatcac
    21181 atcttgcttt attggatcgt tttggagatg tagctttcca acagaagaaa aagagttatc
    21241 agcaagaata tatctcttat cgtgagctgg aaaaaagggt acgaaaagtg cagcaaaatg
    21301 aaaaatccta tgttcagaga atagatatgc tccatttcca gcaagaagaa atagctgctg
    21361 ctgatttgca agtcggagaa gaagagaaat taaaagaaga aagagaaaaa cttagcaatt
    21421 atcaaaaaat tgctgatgga ttagctgcag gttacggagc actgacgaat aatgaacaaa
    21481 acagtgtaga tggtgttggc ttggccgttt cagaactgca agggatcgcc catctagatg
    21541 tggagtatga agcaatttat gaaaatatcc aaagtgctta ctatttgctg caagatgcaa
    21601 tcggcgatat gagtcgtcag attgatttat tggagttaga tgagagtcgt ttagaagaag
    21661 ttacgcaacg tttagaatta atccgacagt tgaaacgtaa atacggcgaa tccattgagt
    21721 caatattggc ttactatgac gaaatcaccg aagagctagc ttcttctgat ttttcagaag
    21781 gtcaattgga caaaatgaaa gaggaactag agcaaaaaga gcttctactt cagcaacaag
    21841 ccgcagatct tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa tccattctgc
    21901 atgaattgaa aagtctgtat atggaaaaca ctgagtttga ggtacgtttc ttaaaagaag
    21961 aaaatagaca attgaatcat gatggatttg accaaatcga attttacatt acgaccaatc
    22021 caggagagcc actgaagcct ttggtgaaag tcgcttctgg tggcgaactg tctcggatgt
    22081 tgcttgcttt gaaaactatt ttttcttctg agcaaggtgt gacaagtatt atttttgatg
    22141 aagtggatac aggtgtgagc ggaagagttg cacaggccat cgcagataaa atttctcaaa
    22201 tatcgaaata ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct gcagtcgccg
    22261 actatcaata ttatattgtg aaagcagtga ttgatggacg tacgcaaact tctgtttcag
    22321 agttgaaaac aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga agtgagatta
    22381 caaaactcac actcgaacat gcaaaagaac tattgaaact cgcaaaaagg acctgagcca
    22441 cctcgcagtg gttcaggtcc ttttcagcag ctgatagcta tattttattt ttacatgaca
    22501 ctcattacag cagtcaatac aacagatcca atcgttaaaa tcagcattgt ccaaatgaca
    22561 aattttgtta ctttagcaaa agtacttgtt tgtttttttt cacgcattgt tttcaacctc
    22621 gtcattaatt agttgattca agtgtacaat cttttttcca tttccacaag agggcttttt
    22681 tataaaaact tccctttttt taaataatag gtagttacga tacaaataaa taatgttaat
    22741 agaactagcc atataaaggc atcatccctt tcagcaatag gtaacttaat gttcattcca
    22801 tagatactgc cgacgattgt cggtatagtt aaaataatcg ttaaagaagt taaaattttc
    22861 ataatattat tcagattatt cgatacgatt gctgaaaaag tctgattcat ttgttctact
    22921 agttttaatt gaatgcgaga agtagtcatt gcctgctctg tttccaccaa tacatcacgt
    22981 aaatgatttt tcaaagcatg attttcttgg aaaagtttat tttgcgctag ttctttcaat
    23041 gcatctaaat tagcagaagt cgcttctctg aaataaacga ggcttttttg gatatccatg
    23101 atttgataaa gttgtttgtt ttcagtggat acttgaagtt tggcttccaa agtatcgcat
    23161 tgagattgaa gcgtagctaa ataactgttg taagagagtg tgacctgcca aatgaattga
    23221 aagaagatat tgagattagg cgaaatatca ctttttggaa aggtctggct aaagactttc
    23281 tttaaaaaca gtggttcatg attcgtaatt gtgataagct tctttttggg cgtcaaaata
    23341 atagagagcg ggtatgtatc cacttgaaaa taaccgctag gacttgtttt agcaaaagga
    23401 tattgtaaca ataataacgc tggctttgtc aactttgatt gattgaattc ttctgtacgg
    23461 gaattttctg catcgtccaa tatggaagtg atgtaatctg acggaatttc aaattgatgt
    23521 gtcagctgat gaatctcttc ttgattagga cgttctacac aataccagat cgtttggtca
    23581 gtttcctttc cggtattacg cagtttctca ttatctaatg taaaatattt gatcatgatt
    23641 ttcctcctgt tgcctgtttc ttttattata acggataggg aattggaata catggaatgt
    23701 gtttgtaaga tatagaatat aactgcataa atttaaataa aatgaaacag ttgtgtaaag
    23761 tatatacagt atacttgaag caacgataaa aaaggagttt acgttcatgg gcacattcac
    23821 accacttatc ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt gccaatttct
    23881 tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt tggcgattcc
    23941 ttttttgtgg ggcggtatgc atgcattgtc tagaaataca gggacaattt ctatcttacc
    24001 gtttttcttt atttctgttt tgctaatggg aatttgtatt gctgttttcc aagcatatta
    24061 ttatgaagaa attatttatc cgcgttattt caagatgact tggcgtttgg tttttctgtt
    24121 gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata ctttcttacc tatgaaaaaa
    24181 atagaaaagc tgtaaaaagt cttttaaaag gctttttgca gcttgatttt taaaaaaatg
    24241 tttatgaata aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa agtgggggat
    24301 tgtggtagac taattttagt tagtggagga atggggggct ttcaatgttg atgggtgaat
    24361 tccaacataa tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac
    24421 ttggcgaaaa gtttgttctg acacgcggat tggatggctg tcttttcggc tatccgatga
    24481 gcgaatggga aaatctggaa gctaaactga acgaaatgcc gttggctaaa aaagacgcac
    24541 gtacttttgt gcgattcttc tattcggctg ccactgaatg cgaacttgat aaacaaggac
    24601 gaatcaatat acccagcacg ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca
    24661 tcggtgtatc taaccgaatt gaaatttggg atgagacacg atggcaagat ttttctgcag
    24721 cagcagaaga gaactttgac gaaattgctg aaagtatgat tgatttcgga ctataagaat
    24781 aggaagaggc tgactaatga ctgaaacatt tcaacactat acagtcatgt tgaaagaaac
    24841 ggtagatgga ttgaacatca aaccggatgg cgtttatgta gactgtacac tcggcggtgc
    24901 cggacatagt gaatatttat taagccaact aagttctaaa gggcatctgt atgcgtttga
    24961 ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta gcttcttatg tcgaacaagg
    25021 tatggtcaca tttatcaaag cgaacttcag agaattagaa gaacgcttga gtgagcatgt
    25081 agataaggta gacggcattt tgtatgatct aggggtttcc tctcctcagc tagatgaagc
    25141 agaacgtgga tttagttatc atcaagatgc accgttggat atgcgcatgg atcagtcagc
    25201 tcctttatcc gcttatcacg taattaatga atattcctac catgaattag taaagatttt
    25261 cttccgctat ggcgaggaga aattttccaa acaaattgct cgtgaaatcg aacgagtacg
    25321 aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa atcattaaac aagtcatccc
    25381 agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat
    25441 tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa tccggttatt
    25501 gaaagtcaat ggaagaatca gcgtaatcac attccattca ttggaagaca gaattgtcaa
    25561 aagtatgttt aaagaatata gtacggtaca agatctacca ccgggacttc ctgtcatacc
    25621 tgacgaattc caaccagaac tgaaagtcat tactcgtaaa ccaatccttc cttcagagga
    25681 agagctaaca gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag aaaaaataag
    25741 aatgaaagag gagtagaatg atggcagaat taaagaaaat gcaagattac gaatatgatt
    25801 tacctgaatt ggtggatcag ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa
    25861 tcattcttcc acaatcaccg gcaaaacgat taaagcagat ttctgctttg gaaaaaattg
    25921 ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt tttaacagtt atgctgcgca
    25981 caaatatcag cggagctgaa aaagaaatca cgtcaataca aacagaaatc aatgataaaa
    26041 agcaagaaaa aacgagttta gaacaagaaa aaaacgaact ttctcgtact gaacggatta
    26101 aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga caatttaagg aaagtgaagt
    26161 aaatgagtct taaaaataaa attcggcggt ttatggaaaa gaaaaatcta aatccgatga
    26221 acaatcgcaa aaaagtaggt atcattttat ttgctacgag tattggattg ttctttttat
    26281 ttgccgtccg tttctcgtat atcgttattg gaggtcatgt tgctggtacc tcattagctg
    26341 aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt
    26401 atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt aaagcgatac
    26461 tctcaaaaac atatacctct ggggataaaa aactgtatgt tgaagaaaag aattttgata
    26521 aaatagcaga aatcctgcat aagaatcttt ctatagacaa aaaagatgca ctgaatattt
    26581 tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat gggaaaaata
    26641 tctcccaaga aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc
    26701 tttattttgt cgaccatcag gctcggatgt atccaaatgg cgtcttttct tctcatttta
    26761 ttggatacgc agttccagac aaagatgaga atggcttagt tggtaaacta ggtttggagt
    26821 ctgcttacaa tgatatcttg agcggtaaag atggtaagat tatttatcaa aaagataatt
    26881 ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa agctgtcgac ggacaagata
    26941 tctacacgac tttagacagc agactacaaa gttatttaga aacactgatg gatcaagtaa
    27001 atgaagaata tcagccagaa gaattgacag cagtcctgat gaaagcaaaa accggagaaa
    27061 tcttggcaat gggacaacga cctactttca atcctgaaac aatggaagga ttgactggaa
    27121 aagatgctat ctggcgtaat ttcttggtac aagatagtta tgaaccagga tcaaccatga
    27181 aagttttcac tactgcagct gctatcgaag aaggagaatt caacgagaat gaaaccttcc
    27241 aatctggaaa aatccaagta gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag
    27301 gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg
    27361 aacagcgatt aggcggacga tggtataact atttacaaaa actgggattt ggacaaagta
    27421 ctcattctgg attagatgat gaagtaaacg gtgctttacc tacattaaat attgtcgacc
    27481 gagcaatgag tgcttatgga caagcggtag gtgtgacgaa tttccaaatg atgaaagctt
    27541 tcacttctat tgcgaataac ggtacaatga ttcaacctcg atatatcagc aaagtggtgg
    27601 atccccaaac tggagaagag cgaacaactc aaacagaagt tctagggcaa cctttttcaa
    27661 aagaaacaac agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg
    27721 gtagtgctta tggtgtatat agtgtacctg gatacaatgt atcggcaaaa aacggaacag
    27781 cccaaatcgc ttctgataca gggggatatc aaacaggaga tacagcctac ctgtattcga
    27841 ttgtggagat ggttccttct gaagatccag actatgtctt gtatctaaca atgaaacacc
    27901 ctaagacata tgatcgaatg gcattggcta aaatcgcaaa tccattgatg aaacgggcaa
    27961 tggattttaa agaaactgaa gaagattcag atacagaaac aaaaacagaa aaagtatccg
    28021 tagccgatta tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac
    28081 tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa acaatctact gccaacggtg
    28141 atcagttgat ctctggagaa aaactgatcc tatatactgg tggtgataag ctgatgcctg
    28201 acgtaacagg atggtcaaaa gcagatatca tgaagctggg gaaaatcctc ggtattgaag
    28261 ttagttttga tggagatggt tattgtgtca aacaagaact tgctccatat gaaaaaatca
    28321 ctgaagacaa acttagtttt actttagaag aataagaaga gaaataggag agaaaagagc
    28381 atggaatgga cacaagcatt gattcccatt gtcagcagtt gtgcaatgac aattgccgca
    28441 atgccgctat ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag
    28501 ggaccaaagt ggcacaatag caaagccgga actcctacaa tgggcggctt agtgtttctg
    28561 atcggctcta ttttgacagg aatttgggtt ggagcttggc aaaaacagct aactcctact
    28621 cttttcatct tgctttttgt attagcgtta tacggagtaa tcggctttct tgatgatttt
    28681 ataaaaatat tcaagaaaag aaacatggga ctgaactcga agcagaaatt gattggacag
    28741 attctagggg gaatcatctt ttatcttgtt taccgttctg aaggttatcc aggtgtattg
    28801 aatttcttcg gactagatct acctttagga atcatttatg gtattttcgc tctcttctgg
    28861 cttgttggat tttcaaatgc agtaaatctg acagatggca tcgatggatt agttgctggc
    28921 ttaggaagta tctcttttgc agcttacggt ctaattgctt ggcatcagca tcagtatgat
    28981 gtattaatca tttgcttaag tgttttagga ggtttactgg gtttcttcgt ctacaacaga
    29041 aaacctgcca agatttttat gggagatgtc ggttcattag cgttgggcgg acttttagcg
    29101 gcaatttcta taatgctaaa ccaagaatgg acattgcttt tgattggttt gatctatgtt
    29161 atggaaacag caagtgtgat gcttcaagtt acttccttta agcttacagg aaaacgaatt
    29221 tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt
    29281 gatacagttt tttggttgat cagcattgtg acttcgttga tcacattatg gatcgtttgg
    29341 taggagatga cgggaaatga aaaaaatatc aacatatgaa aataagaaag tgcttgtctt
    29401 aggactcgca aaaagcggag taagtgcggc caaattatta catgaattgg gcgcacttgt
    29461 caccgttaat gatggaaaac cttttgacga aaatccggaa gcacaagagc tgctttcttt
    29521 agggataaaa gtaataacag gtagtcatcc aatcgaattg ttggatgaag aattttcatt
    29581 gatggtcaaa aatccaggga ttccctacag tcatccttta gtagcaaaag cccaagaaat
    29641 gggtattccg gtaattacgg aagttgagct agcttacgaa gtagcggaat gcccaattat
    29701 tgggattaca ggaacgaacg gaaaaacaac gacaacaacg atgacaggac tactactgaa
    29761 tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat attgggtatc cagcaagtgg
    29821 tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg gaattgtcta gcttccaact
    29881 catgggaatc acagatttcc gccctcatat agcggtgatt accaatatct atgaagcaca
    29941 tattgattat catggtacaa gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa
    30001 catgacagaa aaagattatc tgatccttaa ctggaatcag tcagaactac aagaattagc
    30061 gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt
    30121 gtatgctgac gattactcaa tatactataa aaaagaaaaa ataatggaaa tatctgaact
    30181 tggcgtacca ggtaagcata atgtagaaaa tgcactagct gctatttctg tagcaaaatt
    30241 gtatggtata tcaaatgaag ctatacgaga aacgttgcat tttttccatg gcgtaccaca
    30301 tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt tataatgatt caaaagcaac
    30361 caatatttta gcgactaaaa tggctctaag tggatttgaa acgtctaagg tcgtgttatt
    30421 ggctggcgga ttagacagag gaaatacatt tgatgaatta attccttcat taaaaggaat
    30481 caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc
    30541 aggcatcgag acaatagtaa cagccgattc tgtagaacaa gcagttcctt tagcattaga
    30601 aaacagtaca gatggtgatg tagttttatt atcaccagcg aatgccagct gggatcaata
    30661 cccaaatttt gaaactagag gaaacagatt catggaagct gtgaaccgat taaaatagaa
    30721 aaagtagaag cgagcgataa aaaatgaaaa ttttggttac aggcggagga acagggggac
    30781 atatttatcc agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat
    30841 tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat acaggaatgc
    30901 ctttccatac cttggagatt caaggattta aacgaaaaat ttccatgcat aacatcaaga
    30961 cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt gattttcaac
    31021 cagatatcgt tatcggtact ggaggatatg tttccggagc ggtagtgtac gctgcagcca
    31081 aaatgggtat tccaacagtt atccatgaac aaaacagtgt accaggtgtt accaataaat
    31141 ttttaagccg atatgtggat cggatcgctc tttcatttaa agatgcggct tccttttttc
    31201 ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca agaagtagca gatactaaaa
    31261 aatcagatat tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc ttgatttttg
    31321 gcggtagtca aggagcactt aaaatcaatc aagcagtatc agttttttta agtgaatata
    31381 ttccttcttc ttaccaagtt ttgtatgctt caggagatag atattatgaa gagatcctaa
    31441 ataaaataaa tcctgaaata caaaatatta gtgtccagcc ttatatcaaa aatatggcag
    31501 aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat
    31561 tcacagctct ggggttaccg gcagtgttgg ttcccagccc atatgttacg aatgatcatc
    31621 agactaaaaa tgcaatgagt cttgttcacg caggagcagc aaaaatgatt gcagataatg
    31681 aactaacagg tgaatcacta agtcagacag tcaacgaaat catgggtgat gaagaattgc
    31741 aaaagcaaat gtgccgagct tctaaagaac aaggaatccc tgatgcttca aaacgtcttt
    31801 atgacttagt aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa
    31861 agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac aatttgactc cttggcaaaa
    31921 ggctaaccga aaatatctgg cagaacaaga gatgaaaaac caagagccaa aaaaagaaga
    31981 cataacatta gatacacccc tttcagattc tgaagaagaa aaaacggttc aagaggaaca
    32041 gcaagaagaa agccagtccg agaaaaaaat cgaatttttt gaagagatca agcaaaagaa
    32101 aaacggtgga ccatataacg gttctttttt gaaccggctg cccaatttaa aaagtcaacg
    32161 gcacaaagta ctatttcgtc ggttagcttt gatcatcact gtattgggta taccgctgat
    32221 ttttttgatc tattatgttt ctccttatag caaattacaa gcagttgcag tatcaggcaa
    32281 taagacagtc aacagccaag aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt
    32341 ttggggacag tattttcatc gatctactta tatcgatcgc cttaaaaaag cacagccaag
    32401 aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga
    32461 atataaagag attgctttga tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg
    32521 aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct tggaagagtt
    32581 caaagactct gcaaaaatca aagaattaac aaagcaatac aatcagttat catcagaatt
    32641 acaaaaagca atttctgaaa tcaagtacac gccaagagca agcaataaaa atctgatcca
    32701 attgaatatg aatgacggca atcaagttat tgtaaatatc aataatcttg cgaaccaaat
    32761 gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt
    32821 tggtatcttc tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg gccaatcgga
    32881 agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga
    32941 aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac ggatcatctg attcaaatga
    33001 ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca actccgtcaa gtttaaatga
    33061 aaattgataa aacaacttta aaaagtaaga atgtactttc taaaaaccag taatttatgg
    33121 tagaattgga agcagtgaat atatctatta ataagtatat tttaaaacgg acgctagttg
    33181 ttcgcttgta tatggaatag taagtaggag ggaatcccat tcatggcaaa aacaggaatg
    33241 tatgtaggcc ttgatattgg aacgacatct gttaaagttg tcgtggctga atatatcgac
    33301 agccaaatga atattattgg agtaggaaac gcaaaatcag aagggattaa ccgaggcatt
    33361 atcgttgata tcgataaaac ggtccaagcg atacaacgag cagtacgaca agcagaagaa
    33421 aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta
    33481 gaaaattgcc agggaatgat tgcagttaac ggagattcaa aggaaatcac cgatgaagat
    33541 gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg
    33601 tcaatattgc cacaagattt tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga
    33661 atgatcggcg taagattaga aatgtacggt ctgttgttta caggacctaa aacgatcatc
    33721 cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact
    33781 cctttagcgc tgactgaatc aattctttct gacggagaaa aagattttgg tacgatcgtc
    33841 attgatatgg gtggcggcca aacaacaacg gctgttatgc atgataagca attgaagttt
    33901 accaatcttg accaagaagg tggcgaattt gtcactaaag atatctctat tgtattaaat
    33961 acgtcattta acaatgcaga agcgttgaaa ataaactacg gggatgcata cccagaacgg
    34021 acatcaccag atgaagaatt tccagtagat gtgattggtc agtcagaacc agtgaaagtc
    34081 gatgaacgct acttgtcaga aattatttca gctcgaatgg aacagatttt caacaaagca
    34141 aaagatgcat tggaccaaat cgaagcatta gagttgccag gtggcgtcgt attgacaggc
    34201 ggagctgcta gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta
    34261 aaattgtatg ttccaaatca catggggctg agaaacccgg tatttacaaa tgtaataagt
    34321 atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg
    34381 ggtgaaacct cagcagctcg tcaaatggta gttgaacaag aaacaacaaa cacatatgag
    34441 agctatgaag cacctgaaga aacaatctac gatgagccgg aaccaaaaga gtccggagaa
    34501 aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt ttgattaatt catcctggga
    34561 ggaataagat atggaattct caattgataa taatatcaat gacggcgcag taatcaaagt
    34621 catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa
    34681 aggtgttgaa ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga
    34741 aacagttatc caattaggcc ccaaatatac tcgtggttta ggtgctggtt ctcagccaga
    34801 agttggacaa aaagcggcag aagaaagtga acaatcctta cgcgaagctt tagacggtgc
    34861 agatatgatt ttcatcactg caggaatggg tggaggaacc ggtacaggtg ctgcaccaat
    34921 cgttgcaggt atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga cacgtccatt
    34981 cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga
    35041 aaatgtggat acattattaa ttatctcaaa taaccgttta ttagaagttg tagataaaaa
    35101 gacaccgatg cttgaagctt tccgtgaagc agataatgtt ttacgacaag gtgtacaagg
    35161 tatctctgat ttgatcacag cgccaggtta cgttaacctt gactttgctg acgtgaaaac
    35221 agtgatggaa aaccaaggta cagcattgat ggggatcggt gtggctagtg gagaagatcg
    35281 cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg cttgaaacat ctattgatgg
    35341 agctgaacaa gtacttctta acatcacagg cggtttggat atgacattgt tcgaagcaca
    35401 agatgcttca gatatcgtag caaatgcagc aactggcgat gtaaatatca ttctaggtac
    35461 atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga
    35521 tgaatcaaaa aaggaacgca agtctagccg accagctaga caagcacaaa tgcaatcacc
    35581 tgctcaaaag actgtattag atatggatca agctaaacca acttcttcag aagaagaaaa
    35641 cagttttggc gattgggata tcagacgtga acaaaatgtt cgtcctagag ttgatgattc
    35701 caattttgac aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc
    35761 tcatggcgat gatgaattaa atacgccacc gtttttccgt cgtaaaaaat aaaggagagg
    35821 aagagcacat gattgctgac aacttgcaga aaatcaagca agaaattcag gattcgtgtg
    35881 ctcttgttca tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa tctgttgaat
    35941 ccgctcaagc tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag
    36001 ataaattatt agaaaagaaa caagatttat ttgattttcc tgaaataaag tggcatttaa
    36061 ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat tactttcatg
    36121 cgttggacag tttaagatta gcagaagaaa tccagaagcg gttagagaaa gaattatcct
    36181 gttttattga agtaaatgtt tctggtgagg aatcaaaaca tggaatttca ccagatgagc
    36241 ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat caaggttgtt gggttgatga
    36301 ctatggcgcc taaagaagct tctgatgagc aaatccataa agtgtttggt acgctgaaag
    36361 aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt gaattaagta
    36421 tggggatgag tcaagacttt caaattgcga ttgaagaagg tgcaacattt atccgtgtag
    36481 gaacagcgct attcaaagac gaataaagga ggaacaaaaa tgccgctttt aaacagagga
    36541 gcaattgcta attttttcgg attagctgat gaggaagagt acgaatacca agattatcct
    36601 gaaacaacaa aaaaggctgt acagccaact aaaaaaaata caacacagaa tcaatcaact
    36661 cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta
    36721 acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct
    36781 acaaaaactg tagcgaaaga aagaacagct tcaagaacct ctacacaacc taaagcaaat
    36841 cttgctggaa aaatcactgt aatggaacca agagcttatt ctgaagctat ggcgattgct
    36901 aaacgaatca ttgctggaga agcaacctta gtgaattttc atttagtaga agaaaaacaa
    36961 gcgagaagaa tcgttgattt tctaacagga acagtttatg cactagatgg agatatcaaa
    37021 cgtgttggag atgagatttt tctttgtaca ccgactggga tggagattga cagttcaacc
    37081 gctcaatctt ttgcagatgc gaatttgttt gatctgtaag aggaggaact acgatttata
    37141 ctattgcact attacttcat ttacttcaac gggcagccta tttctatagc attatcttga
    37201 tggtatatgc attgctctct tggtttccag gcgggtatca atcagggcta ggacgattat
    37261 tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg aggattggac
    37321 ccttggattt cacaatttta ggggcaatcc tgatcttgaa cttagccgta caaggtttag
    37381 cacagatcgt tatatggata actgtccatt tagtttattg attgaagcaa caaaatcttt
    37441 attctcagaa tagataggag gggaaaattt tggatgcaaa tgtataccag cactttcggg
    37501 cagatgaacg tacatttatc gattcggtag gcgattggat tgaacaggtt caaggacaat
    37561 atgcacccta tctgaccgaa tttcttgacc caagacaatc ctatatttta gaaacactga
    37621 ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg atatgaacag gcagaaagaa
    37681 aaagatgttt gattttcccc gattattacg aacctgtaga ggaagatttt gaaacagcat
    37741 tgtatgaaat ccattatcct agcaaatttg ccagcctaag tcatgggaaa atccttggaa
    37801 ctttagtggg aacggggatc aaacgctctt actttggcga tatcatctcg gacggtgatc
    37861 gctggcaagt atttattgca aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga
    37921 ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt atgccgaaag
    37981 atagctggac acaagaaaga ggaacagtta gttccatgcg actagacagt gtgatctctg
    38041 agatttataa tatttccaga cagcggtcca agcagctgat cgaagctggt aaagtaaaag
    38101 tgaactggac agaaaataca agacctgatt ttgtacttga actattagat attatttcag
    38161 tcagaggtta tggcagagtc cagatccaag aacttgaggg gaagacgaaa aaagaaaagt
    38221 acagggtact gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt
    38281 aactccatta gatatccaaa ataagacatt cccgacaaaa atgcggggat ataatcaaga
    38341 cgaagtagat gatttcttag atttagttgt tcgtgactat gaggaattga ctcaaagaaa
    38401 tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt
    38461 aaaagatgct ttgaaccaat caatcatcgt agctcaagat acggctgata aagtaaaaac
    38521 aagtgcaagc aaggaatccg aagtgatcgt tacttctgca caaaacaaag cagatgagtt
    38581 agttgcgaac gctgaaaaac gtgcacatca attaacaact gatgcagaag aaaaagcaag
    38641 aaaaatcttg acagatgcaa cagaaaaagc aagacagctt gcgacagaaa ctgaagatct
    38701 taagaaaaaa acacgagtat tccatcaacg tatcagcttg atgcttgagt ctcaattaga
    38761 acaagtgaaa agtccggaat gggatgaaat tttacaacca ttctccagct acgtgactga
    38821 cagccatgaa gtgatcaaag aagttttagc aaaacagctt gacaatgaaa atgatactga
    38881 tgtaaactca gacacaggag tcatagaagc acctatgacg gcatttgata cacaggctat
    38941 tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag cactttgtta
    39001 ttcttaaagc gagccgatga gagtggaagt tcggtaagac actgcatggt gaaagatcct
    39061 ctacttgaga cctgcttttc cgaaattaga gtaagaagca gcggctgacc tcgttattgg
    39121 tcacagagag tttgcactag atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg
    39181 tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag tgtatggttg
    39241 aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt
    39301 gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag
    39361 acaagagttg aacgaaggaa aacctagctt tgttcttcat gatggacctc catatgccaa
    39421 tggtaatatc catttaggac attcattgaa taaaatcagt aaagacatta tcattcgttc
    39481 aaaatcaatg tctggctatc gtgcaccata tgttccagga tgggatacac atggattgcc
    39541 gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata
    39601 cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat aagcaacgag cagatttcaa
    39661 acgcctagga gttgcaggag attgggaaca tccttatgtt acattagatc cttcttatga
    39721 agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt
    39781 gaaaccaatt tactggtctc cgtcaagtga gtcttcttta gcggaagctg aaattgaata
    39841 taaagatgta aaatctccat ctatttttgt agctttcaat gtcaaagacg gcaaagatat
    39901 tcttgatgaa gatacagctt ttgtcatttg gacgacaaca ccatggacat tgccagcaaa
    39961 ccaaggtatt gctgtaaatc caaaatttac gtatgtattg gtggaagccg atggccgtaa
    40021 gtttgtcgta gcgaaagatt tgctagaaac agtccaatca gaaatcggtt gggaaaatgt
    40081 aacagttcta aaagaatttg ctggacaaga aatggaatat atgactgcaa gtcatccatt
    40141 ctatgaacgt gaatcacttg tgattttagg cgatcatgtt actttagacg ctggtactgg
    40201 attagtccat actgctccag gacatgggga agatgactat atcgctggta gcaaatataa
    40261 actatctgta gtgtcacctg tagactcaaa aggattgttt acagaagaag cacctggatt
    40321 tgaaggtatc ttttatgaca aagcaaatcc tatgatcact gatctattaa aagaaaaagg
    40381 agctttgctg aagctagatt tctttactca tagttatcct cacgattggc ggacgaaaaa
    40441 gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct atcgataaat tccgtcagaa
    40501 tattttggat gaagtagaaa aagtagagtg ggttatccca tggggtaaaa cacgtttgta
    40561 taatatgatc cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat ggggagtacc
    40621 acttccgatt ttctatgctg aaaatggaga agcaatcatc acaccagaaa caatcgaaca
    40681 tgtggcaaaa ttatttgctg aacatggatc aattatctgg tttgaaaaag aagcgaaaga
    40741 acttcttcct gaaggattta cccacccagg ttctcctaat ggcgaattta caaaagaaaa
    40801 agatatcatg gatgtctggt ttgattctgg ttcttctcat gaagctgttt tacgtcagcg
    40861 accagaatta tcattcccag cagatatgta tttagaagga tcagaccaat atcgtggctg
    40921 gttcaactca agtatcacga ctagtgtggc tatcaatggt gtagcaccat acaaatcagt
    40981 tatttctcaa ggatttgctt tagatggtga aggccgtaag atgagtaaat ctttaggaaa
    41041 catcattgca ccagaaaaag tgatcaaaca atttggtgca gatatccttc gcttatgggt
    41101 atctagtgta gattatgaag cagatgttcg tgtttctatg gatatcttaa gtcaagtggc
    41161 agaagtatat cgtaaaatca gaaatacgat gcgtttctta ttagcgaata cagaagattt
    41221 cgatcctaaa aaagatactg tttcgtacaa tgatttacgt tctgtagaca aatatatgac
    41281 cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca aatataactt
    41341 catgcatatt taccgtacag ttatgaactt cttgacggtt gatctatcgt cattctacct
    41401 ggattttgct aaagatgtag tttatattga agcagaagat gattatcaac gtcgttgcat
    41461 gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa ttattgactc ctatcattcc
    41521 gcatacagca gaagaaatct ggtcattcct gaaagaagaa gaggagtacg ttcagttaag
    41581 cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa ttgatggata cctggactgc
    41641 tttcatggac ttccgtgata atgtcttaaa agcattggaa gaagcaagaa acagcaaatt
    41701 gattggtaaa tctttggaag caaaactaac tgtttatcca aaacaacaag ttcgtgaaat
    41761 gttaaaagct ttggatgcag atattgcaca attattgatc gtttcaccag actactttga
    41821 agttatggca gcagatgaac aagtgccaga taacgctatg gtatttgatg atgttgctat
    41881 tacagtggaa aaagcagacg gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt
    41941 aggagtagat gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt
    42001 ctatcctgaa gctgtagctg aaggattcga agaaaagtaa aagggaaaag ggggtgtgac
    42061 atgttctttg tcacaccccc ttttgttatc aatcgtttaa ttttccacgt tcacgatacc
    42121 agttatctgg ttgccagatc cattcgaatc cgtctttttc aagtagttcg aaagctgctt
    42181 taggtcccat tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt
    42241 ttcgaatgac atccacgata tgccaagatt gagcgacttc ttcccaatga gagaagttcg
    42301 ttccatcacc attcaacgca tctaatagta atttttcgta agcttctggg ctattttcaa
    42361 ccatttctgc actatttcta aattccaatt tgattggttc cgtttcaaat ccttgcccta
    42421 cttctttccc attcaaggat aaagaaaatc cttcagttgg ctgaatatag atcgttagaa
    42481 cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac agatgttttg aatacattaa
    42541 caggaacttg tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg
    42601 tacgtacata gaaaggaaca cctgaccaac ggaagttatc gatagtgaat ttgcctgcga
    42661 cgaatgtttc agtctctgat tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt
    42721 gtacaccatc caattcacct tgagcatatt gcccgcgaac gaagttttct aacacttctt
    42781 cttcgttata aatgcggata gctttcaatg ctttgatctt ttctgtacgg atttcttttt
    42841 cggaaaatgc tactggcggt tccattgcaa gtaatgctac aacctgtaaa atatgatttt
    42901 gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc cccgcgatct tctacaccga
    42961 ggttttcgcc aaaagtaatt tggatattgt cgatatagcg attgttccat tgagcctcaa
    43021 aaatattatt ggcaaaacgg atagctgaga tgttttggat catttctttt cctaaataat
    43081 gatcaatcct gaaaatatct tgttctggga atacttctct gatttgctgg ttcaattcaa
    43141 aagcagactg gtaatcagaa ccgaacggtt tttctatgat caaacgattg aagccatttt
    43201 ttgtcataat attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca
    43261 tagccaaata atagacacgg ttaccgttta attgatagtg tccatctaat cggtcagaca
    43321 attcttttaa tgtctgataa tgcgttgaat catttacatc atgggattga tagtaaaaat
    43381 ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa tgcacggatt gtttctttga
    43441 caacttcacg gtagtgctca tcgctccagg gacggcgagc ggttccgatt acagcaaaat
    43501 gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg
    43561 ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt tttttcatta gacatgtgga
    43621 tacactcctt tatattttat cgacaaaaat cgtgctggcc gccttaaagc tgacggggat
    43681 tttctttcta tcattttgga tagtgatcgg tccttcataa gctgcgatgt caacgatctc
    43741 atattcttcg ttgatattca ggtcaatagt gactaagtaa tccaacaatt ctttttcatc
    43801 aagcactcgt gcgatacgca cttttgtacc gacaggatag gactccaatg tttgtcggcg
    43861 ttcttcatgg acaacttcct cttttttagg aatcgatcct ccatgaggac aatgttcagg
    43921 atgattcaaa tagttttcta aatgatctgc aagatgttcc gaagtcacat gttccaatac
    43981 ttcagcatca tcatggactt cattccagct atagtctaaa tgttcaacta aaaaaacttc
    44041 ccataagcga tgtttgcgaa tcagactgct agctttttgc agtccgcttg cagtcaattg
    44101 tacaccttga taaggggaat gttctacaag ctcttctttc acaagtttag aaatcatttc
    44161 actgacagat gcagctgaga catctagact agatacgatt tgtttattgt tgactttcgt
    44221 tgtatctcca cctagttcta aaatgatttt caaataatct tctcgatttg gtgtcatatt
    44281 cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt
    44341 gactattttt ctaaataaaa aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa
    44401 gatgatagag aagactcatt ctttcgttta taaaggatat atatatacat tgggagggat
    44461 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
    44521 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
    44581 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
    44641 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
    44701 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
    44761 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
    44821 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
    44881 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
    44941 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
    45001 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
    45061 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
    45121 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
    45181 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
    45241 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
    45301 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
    45361 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
    45421 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
    45481 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
    45541 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
    45601 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
    45661 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
    45721 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
    45781 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
    45841 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
    45901 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
    45961 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
    46021 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
    46081 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
    46141 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
    46201 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
    46261 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
    46321 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
    46381 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
    46441 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
    46501 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
    46561 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
    46621 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
    46681 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
    46741 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
    46801 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
    46861 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
    46921 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
    46981 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
    47041 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
    47101 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
    47161 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
    47221 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
    47281 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
    47341 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
    47401 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
    47461 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
    47521 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
    47581 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
    47641 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
    47701 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
    47761 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
    47821 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
    47881 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
    47941 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
    48001 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
    48061 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
    48121 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
    48181 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
    48241 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
    48301 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
    48361 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
    48421 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
    48481 gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca actgctcttt
    48541 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
    48601 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
    48661 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
    48721 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
    48781 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
    48841 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
    48901 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
    48961 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
    49021 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
    49081 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
    49141 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
    49201 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
    49261 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
    49321 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
    49381 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
    49441 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
    49501 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
    49561 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
    49621 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
    49681 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
    49741 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
    49801 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
    49861 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
    49921 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
    49981 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
    50041 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
    50101 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
    50161 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
    50221 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
    50281 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
    50341 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
    50401 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
    50461 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
    50521 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
    50581 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
    50641 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
    50701 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
    50761 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
    50821 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
    50881 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
    50941 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
    51001 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
    51061 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
    51121 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
    51181 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
    51241 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
    51301 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
    51361 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
    51421 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
    51481 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
    51541 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
    51601 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
    51661 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
    51721 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
    51781 atgcttttat cggcatactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
    51841 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
    51901 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
    51961 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
    52021 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
    52081 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
    52141 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
    52201 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
    52261 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
    52321 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
    52381 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
    52441 tgatcattga tttggtgcaa aaatatattg cagaaccatt cctagggtta tctcaagggt
    52501 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
    52561 gatcaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
    52621 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
    52681 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
    52741 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
    52801 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
    52861 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
    52921 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
    52981 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
    53041 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
    53101 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
    53161 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa
    53221 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
    53281 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
    53341 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
    53401 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
    53461 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
    53521 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
    53581 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
    53641 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
    53701 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
    53761 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
    53821 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
    53881 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
    53941 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
    54001 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
    54061 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
    54121 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
    54181 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
    54241 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
    54301 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
    54361 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
    54421 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    54481 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    54541 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    54601 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    54661 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    54721 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    54781 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    54841 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    54901 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    54961 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    55021 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    55081 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    55141 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    55201 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    55261 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    55321 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    55381 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    55441 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    55501 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    55561 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    55621 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    55681 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    55741 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    55801 cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg
    55861 gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata
    55921 taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat
    55981 atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
    56041 aagtatattt actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa
    56101 agtaaattgc ttttcaatcc ctaattcttt taattttttg cgcaaatatt tgctgacggc
    56161 gtttgatgaa actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttgat
    56221 ttcaaaattc tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt
    56281 atgtttatca atggaaattt tcctatttga agtttcgttt tttgtttttc cccattcatt
    56341 taatttataa ttccatgttt tattaatagt gatgatttga tcttcgaaat cgatatcttc
    56401 ccatgttaat cctagaagtt cagcaaatcg cattccagtg gcaccagcaa ctaacacgag
    56461 cataggagaa gagtactgcg cagaaaggtt ttcttctacg agtttcatta aagatttaaa
    56521 ttgatcataa tctaaatatt tatcttcttt tggcttcaat gaatcttttc ctttgattac
    56581 tgcttttcta gttggatcaa acggaattaa tccttcttct acggcatctt tcaacgacgc
    56641 tctaatatgg ttattaaact taataacggt agattttaca tggtctttag catacttatt
    56701 taaaaagcgt tgatatccca ctctatctaa atcagaaatc aataccgctg gcatatattt
    56761 ctttatgttc attaacgtat cttcgtattt tctataagtg ataggagata ctgttccttc
    56821 tttataaagt tgcatccaat cctcaaagta atctgataat aatagatttt tgcgatcccc
    56881 tttaaggcct ttgttcaagt tatattctaa ttcgttggcg gcatcttttg cttctccttt
    56941 tgtcttaaat cctgattttc ttatcttcgt gtattttccg tcatcttttt tgtatgaaat
    57001 ttcgtattgc caactatttc cacgtttgac taatcttgcc ataattgaat cagctctctt
    57061 tctctgatac aataggaact ataaagaagc ccattgtttg ggttttatta ttttttgcac
    57121 atcctttttg cttgccggcg caaggatgtg ctatttgtcg ttttaatggt ataattggat
    57181 gtgtattaga tcatccaatc agaggtgttt attatgatta aagtgatttt taaaagaata
    57241 actgaacttc cagagtttag attatttgtt gattattggt atgtctggtt ttttattgtt
    57301 gcattgctga taggtctaga tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa
    57361 taaattgaat tcctaaatga gatattttgt agcccatatt ttgtttgccg acatcaccta
    57421 atactatgga gctaatcgtt ttttctaaca gaccaaaatc agataatcta tttaagctac
    57481 catctattag ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa ttttcgtttc
    57541 cacgactcat taagcatgct aacaattgaa tctcaatcga tgttaattga ttcagtacaa
    57601 gaagataata ctcctcgtta tcccatgaag cgttgttaat tcgaagtaaa caattcttat
    57661 aaagattttt atacatatct attttatttt gtgcctttga tttttctacc tcgtcattta
    57721 tcccttcgaa tattccaatg agttggtcct tgttctctaa gttgaaaaca tcttctggaa
    57781 tttgatcgcg aatgttttct aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt
    57841 tgaaacgctt ctcgttttta gacccaaaat aaagtgactg cacaacgcca ccaatataag
    57901 gaattgctcc aataccgcct tcaactatta attcaagata ttcttttttg gttagttttt
    57961 cattgctatt atccatagtt acctcctatt ttgtcatttc ctttacaagc attgtgactc
    58021 ctgatggcac tcttttgtat aggttttatt attgtgcaca atctaactct tagtggggcg
    58081 tggttcattt attttttact tactttacta ttaatcatat cccattgagc ataggatttt
    58141 tctatgttcg atcctcttga actaatttct cctgtaaaag ttactttatc gccctttgta
    58201 aaggtatcag cattgtttaa gtccttcgca aaaattacat atgaaacttt aggtgttgca
    58261 gaaatatcat tccaactttt atcatcgtac tctttgtctg ctataatggc tattctagta
    58321 gtcattgatt cgataacagt tccttcaaaa gtatatgttt taccactaat atatttagta
    58381 aaagctgtat tttgctcagt agctggagta atagaataat aactatctaa gaaagcaccc
    58441 cagtcatgat tatttgtctc taaaaattct tcaaattgag ttaggctttt attgctgctg
    58501 ttagatgtag tagtttgagt atcagatgat gagctttcct gtttgttatt gtcatttgcc
    58561 tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa taatcccgat aataaggaga
    58621 atcataggtg ttttcttatt tgtttttttg ataaatctta caattaacat gattaaacct
    58681 ataataaata aaacaatacc agtaaaacca aatataccgt ccattaatag atcctctttc
    58741 tcttttgata ttgaatatta taaaccaagc aattcttttt tcttaaaatc gaattcttct
    58801 tgattaataa tcccttcatc taacaactct ttatactttt taatttcatc tgcacttgaa
    58861 atttgtgttt ctttggaatt attatctaat atagattgtt gcttctctat atattttttt
    58921 aaattttcca tttcattcca atattttttt gtaaacataa cggtgttttc atctttcgtg
    58981 gccgctaata tacctccgcg actttcgttt ccgcctagca aggtaaattg tatataacca
    59041 ttactcatac ctggtttttt taattgtacg gctgatatat ttttaaaggg gatagttttt
    59101 tctcctttaa taccttgatt aactaaattt ataaatcctt ttctaataat tgaaatagaa
    59161 tcatcagtta ctttaatcaa ggtttttcct ggagatttta ttaaaatctc tttttccatt
    59221 ttttcttcct catttctgtg atatgatttt ttttgtagaa gctcatatat gaggaaagag
    59281 cccgtgttgc agcacgggct tttttaatgt tttgctgaaa tcggtttttt taataattct
    59341 tgatgtattc atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag
    59401 acagcataac aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg
    59461 agattaaact gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga
    59521 atagctattt tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac
    59581 aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag
    59641 gcttctgcag tttcaccgta tagattttca ttcaaaaaaa tgattctggg ttttggatag
    59701 tagaaaccag gctctttcat ctccatataa actactttca aattgtattc gctcaacatt
    59761 tctttcaatt tcaaatacat acaaaccatc actccaacta ttcattttcc tctaaagctt
    59821 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
    59881 aaacagtatc gtcttctgat aaatccacat gtttaggggt ttctctttct tctctaccta
    59941 gaaggtagtc tacagagaca tcgaaatagt cagcaatccg acttaattca tctgaattag
    60001 gagtattatt tttccacttt gctagataac cgttagaata tccaaaatta atttctaact
    60061 gccgtataga tatctttttc ctcttcgcca agtcttttat tatttcatag gtattcatcg
    60121 ataaaacaac ctttctaaat gtttacaaaa aaagtttaga aaaatacgca gaaataattt
    60181 gactaattct gagtaatgag ctatactata tcttgtaaac agatttaaca actaaaaaga
    60241 caacaaaaaa cactattgat atgtaaatgc agaccgccaa gaaagcttta aaatcaatgt
    60301 ttttatgtct tatttaatta tgctcttagt atagagtatt actcagatac tgtcaattga
    60361 atttagaaaa aagttgttaa atttgtttac gaatataaaa gaaaggaatg agaaatatga
    60421 acacaccgca aatttttaat ttcgaacaaa acgaagttcg gacaatttta gtaaatgatg
    60481 aaccatattt tgtaggcaaa gacgttgcga gtgttttggg ttattcaaac actaaagatg
    60541 ctttgtcgcg gcatgtagat ttggaagata agatggggtc gcgaatcacg acctcaggtc
    60601 aatcaagaga gatgacaatc atcaacgaat ctggtttgta cagtttaatc ttaaaatcaa
    60661 aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag tgaagtgttg ccagcaatta
    60721 gaaaacatgg aggttatcta actccagaaa aagtagaaga agctttgctt aatccagata
    60781 caatcattca attagcaact caactaaaag aagaaagaac tggaagatta atcgcagaac
    60841 aaaagattgc agagtacgaa cctaaaatct cctatttaga tagcatatta tcttctacag
    60901 attcagtaac aattagtcag attgcagcag attatgggat gtctccacaa cagatgaata
    60961 aattacttca taaactaggt attcagaaaa aagtcggtaa ccaatggtta ttgtgcaaaa
    61021 aacacatgaa ccaaggatac acaaaatctc atacaactga gatcccgaaa gccgatggtg
    61081 gcactaaaat tgtaatgaat accaaatgga cacagaaagg gcgtctattt atctacgaat
    61141 tactaaaaaa agaaggatat taccctcaaa tggatttaga ggaaattggt tagaaaggag
    61201 ttttagtatg actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata
    61261 tgtcgaaagt tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa
    61321 aagctactta tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat
    61381 agattcgatt atcactatgt acgaattgta aagggaggaa cagctaatgc aatatctaga
    61441 agcaaaaatc ccaattccag aaggctatgt aattatctcc caagtggatt atgaggagtt
    61501 aaaaaaagct gatgatactg gtagatggat gacgttgcca gaagtgctag aacggattaa
    61561 cagaaaatat gattggttta cttctagagt tttaaagaac ccaagatata gaaatattat
    61621 cgatatcgag aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata
    61681 tctattttta agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag
    61741 gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt cagcgcaccg ctaatcatct
    61801 acgctttagg actttgggga agcaggcaag cgttgatagg aacgattgtt tacatggttt
    61861 ggatttttat ggggcttgat gaagctgaag ctgagtacag agcgaaaaag ccaaccgagg
    61921 gggctgacta aaaatgaata gaaaagagaa actagaatgt atattactat tactcagttt
    61981 aattctttca ctaatttctc tattgggaag tttgtatttt tgatatcaac aattttgtgg
    62041 atttgggttc tatttagagt aaatatcatt tttgtttggt gattaaggag tttattaaaa
    62101 ttaattttcc ctacttcaaa tgcaaggtaa caagatatag cacttttagg aggaatattt
    62161 agcggtacag cttcagagta tattctatta tcatcgactg atcctccttt agcaattaat
    62221 ttcttaaatg gagtagctga ccatctgcgg tttaagttag cggatgaaag ttctaaatta
    62281 actaacatag ctggttctgt tgaatagtta gaaatgatta cttttgtata aatcatatct
    62341 gcagcgaagt aagaagcatt aagttcaact tctagttgag ggcgcattcc ttttattttt
    62401 atccctgtta aaaacgtact caatataaaa cttattattg acatccattg aaaaattgtt
    62461 aagtgaaaaa aattcaaaat aaacaccacc agtttttaac taaattatac caaaaaggag
    62521 agaagaaata atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagctt
    62581 acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt ttaagagata ttgatgcact
    62641 aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa ggtaatgaac cagattcata
    62701 tggcagaaat cgacgagttt tcttcattag tagagatgga tttttcttgc tggctatggg
    62761 gtttacagga aagaaagcta tctacttcaa acaaaaatac attgaagcat tcaacgaaat
    62821 ggaagatgtt attcgcaaga atactgttcc tcaaacaatt gaagatatga tgatctatca
    62881 attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat
    62941 tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta tgggagttct
    63001 agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg
    63061 gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt
    63121 cgatgatgct gtttcattta ttgaaatgtg gttgccagaa actgcgatcc gtatggaaat
    63181 tgatcaactg aacagacaac agagactttt tggtgatgac aatgaataga gctgaagcgc
    63241 taagaatagg gacggcaatt gctaattgct ggtggaaata ctataaacca atcatcctaa
    63301 gccaacaaca tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc
    63361 aagcaaagag tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact
    63421 ttccactcta gatcaatatt tgattgatcc tgattggggc aagccgaaaa ttgaggaaac
    63481 aagtggtcga aaaatcagac gaaatctttt gacgaatgaa gaactagctt gggatcaaga
    63541 tgatttaggc aaccatgtaa ctatttggga tcatgtttat cttatccatc tatcgaagca
    63601 ttcgaataaa cctgaatata tttacgtcat cgaagatggc ttgattgatg cgctagaaga
    63661 gtacgacaga gataacttga ttgatatctc ttattacgga ccaggtaaga aatacattgc
    63721 tgaaatggag gcagaatttg atgagtgaaa tcaaagggac aacgaacttt gaaaaacttt
    63781 ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa ttttgattat ttatcttggg
    63841 ctcacgcgtg ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt
    63901 acaaacacta cagggttgtt tctggaactc atcaagactt tcttgttgag gaatataaac
    63961 cttttcttat ggatgaaact gggacttatg tatctgtctc agtaacggtt aaaggacaca
    64021 cggaaaccga attatttcct gttttagatt atcgaaacca accagttgtt aaaccaaatg
    64081 caatgcaaat caataactca ttgaagcgat gctttgtgaa agcattggct ctacacggac
    64141 tgggattata tgtatttcaa ggggaagata ttccaacacc acctagaatc gatacaaaga
    64201 aattaaacat gctagagacg attctagaag ctttcaatga gcagatgggt aaagatatga
    64261 ccaaaacctt aatcgaatat gttaatgagc agacagataa attagggctc ttagctgata
    64321 acgttgaaac tattgaacag ttaagctatg agcaatgtgc cttgatggag cgagcaatag
    64381 cagctaagag aaaagaatta gataagaagt gatatgagtg tttaaaccat taatcgattc
    64441 atattcagcg gttctgaaaa agttcaaagg aaaagacata agcgcaacca tcaatgagga
    64501 agtgaacatt gatcgactaa agacgatgta tgacggctac gatggtgatc gagtcattga
    64561 aattcgtttt attgatccta gacgtttcac cgtacagcaa cgaaacttca tctatgcgct
    64621 gataggcgat atttttatcg atacaggcat gccaacggac ttctggaagg aattcttcta
    64681 ctttcgtttt gaaggtgtca cagggcgcaa aataagcctg aaagacgaat cgaatacaac
    64741 tgtgagtgat gccaatgtct tagcaaatat catcttagat ttcatctttg aacatcatat
    64801 tcctttcaaa gaaggctatg agattttacc tgcgaatcaa gaatattact tctacaaatg
    64861 catcacaaaa agagtctgct gcatctgtgg caaaacagga gctgacatcg atcactttga
    64921 caaagcgcta ggaagacgaa agcgcaaaga agttgatcat gcagagtaca catttgcagc
    64981 actctgcaga atccatcaca cagagaagca caaaataggt gtgattaatt tcaaaaataa
    65041 atatcaaatc aaagggatca aattaaacca gaaaacaatc aaaaagttaa atattggagg
    65101 ggaaaaatga cagaacatcg aagttattat gcgattatac cagccaacgt aaggtacgac
    65161 aaaagactta aaccaaatac taagttgtta tacggagaga taacggcctt gtgtaatgaa
    65221 agaggctttt gttgggcagg caatgagtac tttgcagatt tatatggtgt gaataaagag
    65281 accatatcgc gatgggtaag tgatttgatt aagtttggat acttgaatcg ggaaatcatt
    65341 tacaaagagg gtaccaatca aataatcaat aggtacctac gaattaatca ataccctatt
    65401 gacgaaaaac gcaatacccc tattgacgaa aaagtcaaag ataataatac atctattaat
    65461 aatacattta ataatacaaa agaatatata agagagttac cgccttcgaa aaaatcgaag
    65521 gctaagcccg tccgtcataa atacggagag tataaaaatg ttcttttgtc agatgagcag
    65581 atggagaaac tcaaaacaga attccctaat gattaccaag agcgaatcga acgactgtca
    65641 gagtattgtg aatcatctgg taagacttat aaaaactatt tggcaactat tcgaagttgg
    65701 gcaaggaaag aaaaaagtga acctaagaac gcaagcagtg gatacaagcg cacaggaaga
    65761 cgagagaagc ttcctgaatg ggcaatcgac caagaagcct atctcaagaa aaaagcgcta
    65821 gaacgagcta atagacaatc aaaagcacca ttctaagagg tggaaaaatg aagatcgatt
    65881 atctagaact aattaatgaa atagcgaatt ataaaaaggg cgaggaatta gacgtcctga
    65941 gagacgtata tgatcaactc gaagaagctg gaattgaagg aattaagaat gatcgttcga
    66001 gttggagtaa actcagatac tatttcgcac tctatatcga tggaacgcaa ttaagaaatt
    66061 tagcatatac caaattacta ttcattgatt gtgttaaagg cttgcaaaaa catcttaatg
    66121 aacttgagca ggtgtaaaca agatggacct aaagacattt acagcacaga tcgaattaat
    66181 gcatcaagaa gctttaagac aaagtgtgtc gtacgaagac aagtggctca acacatttca
    66241 tggcggacgt gagagcgcac ttgatcaagt actcaaatta ctgaaaggag aatgtcggga
    66301 tggataagaa agcggcaatg cagcgaatta tcgaattgac ttattcagaa gattggcagg
    66361 aagataaaaa aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc
    66421 ccaaacggag aacgccgaga aagattgcaa tctggcatgg tgatcgaatt ctagtaacgg
    66481 gtaccgctaa acagttatct gaaattactg gtctgagcaa aaatattatt tgggatagag
    66541 ctaggagctt atggattgat tcaaaaggaa gacagtttag gtatgtggag gagaaataat
    66601 ggacgaacta atcgcgaaag tagagcagtg ggcaaaagat aagggattgg atcaagcgga
    66661 tttcagcaag caaatgttaa aaacggtcga agaaatcggg gaagttgccg cttctctagc
    66721 tagaaaagat gaacatggtt taagagacgg aattggcgat gtagtagtaa ccttgatcat
    66781 cttggcaatg caaaatgata tggatttata cgagtgcttg gattttgctt atgacgaaat
    66841 caaaggtcgg actggaaaga tggtcaatgg tgttttcgtt aagtccagtg acctgaaata
    66901 attccattaa tggaagaaat aactgaatag gaggatagac atgattgatg caaaacaagt
    66961 gcaaaaacag aaagatggaa tgttgatgtt tgaagcatat gttctaccat ttcttaatca
    67021 atttgaagtt ttggaatgct ctgccagcgg agaagaattg gagtatgttg taattcgtga
    67081 aaccaaagaa aacgttcaga agctaaacga atttctttgc actattaact gttgggatat
    67141 gatagctcca ggatttttat gtccagctat gggagaattt ttggaatatt gtcgtcttga
    67201 agatgcaggc actttggatt tagcttatct ggtatacaac tacctaaaca tcaacacaga
    67261 tcatttgtgg tttggcactg ccgaaagaaa atgggtagtc cgctaaccac caatatcaat
    67321 gaaaaaaggc ggtttcctga aagtggaatt gccgccattg ctgtaaggtt ctttacgcga
    67381 ctcaaggtca cagaaatata aatgcaaatc tcttttgaag tgatccaaag ctctcggaaa
    67441 atggaagttt tttgtttcaa agaataaatg agatttgcgt caagaacctg agagttattt
    67501 ccaaaaaagc ttccaaagaa ccgaatgagc taaggacatc atgtgtaaac acataatata
    67561 gctctccttt cacggctaat cataacatga aaaatattcg atgtcagtag gaaaaaaggt
    67621 gaattatgaa aagaatatta aattatcccg gcagtaacag attctgcaat taaactccga
    67681 agctgatgta tcagtatcaa ctgtagaata ttatgagcgt ggagaaagtc aagcagtcac
    67741 aaatgtaaaa atcgataacg tcaacggtct tatctgttta gatgaaggcg atagtatgaa
    67801 tgaaattgct gcagaccatg aattatcaga aactgcttta attgatcaaa tcgaattagc
    67861 tagacaatac tttgttccat atgcagatac ctggtccaag cataagaatg acatcatttt
    67921 tccagaggaa caatgagagc aactgatcca gtaattatcc ttgaggaagc caaatttatt
    67981 tggactcacg aagagataga gcaagcacgc ttgctctttt ctcaaggagt taagccgagc
    68041 aaagtagctg aaataatggg tcaaaagatt cttgatgtcg gattgctttt gctccatcta
    68101 gcagaaaaaa tttgatttga ggtgaaaatg atgattcaac ttgcaggcat ccaaacaggg
    68161 aaaatttatt tttctggaga aagcaaaagc gaagccaacc aatggctatt gaaaacttac
    68221 acgaacaata agaagctaag gaaaaaatat cctgatttgt tgctaaagga tgatcagatt
    68281 atgccggaac cgatgatttt gacccgtaag gagggaagtt gaaactaaaa aaccagccgt
    68341 gtgggctgga gttgctattt ctttttaata atgagttttt ctaaatttaa ttcattctca
    68401 cgagtttgcg tccaataatc agaataatat tttaattgtt taagagtagg aatgtcgtct
    68461 tcggtaaaat tagaaaataa ttctggagaa ttatcttttt ttaattgttc taatcctgat
    68521 tctttaaaac gttgttcaac gagttttact aaattgttga tattcatgat aaatcacctt
    68581 tttaaattta atttcgattt gattaatcga tacaagtatt atatcaaata aaaaagctga
    68641 tttagggggt cttacaaaaa cactaaaatt ttttcaaaaa gaggaggaac ggtggtgaaa
    68701 gtcagagaac aattcaagat agtgagcgac gaaggtaatg tttacaaaat aaagatcgtt
    68761 aatattaatg aatatcgaga agcagatcac aaatatttgg ttgacttata catgaacgat
    68821 aaatatttgg gagaaggatt ttgtggagat tcactttttg atgatgagcg aattttgaag
    68881 gaggaaacag aatgatccac gaactaaaaa tactaccaga atattttgaa gcagtgacga
    68941 gcggacgcaa acaattcgag atccgcaaga atgatcgtga cttcaaagtt ggtgatcagt
    69001 taatactacg tgaatataaa aaatacatta aacatgaatt acctggacta acgagaccaa
    69061 catttagtta cactggtgac tcatacaaag cagagatcac ttatattact gattacatgc
    69121 agaagtccgg atatgtagtg ttggggatta aaggggtgaa ttaaatgaaa gtatacatct
    69181 tagctatcac agagggcaca tggatgttcc cagtcggttc cggaaaaatc tataaatcaa
    69241 aaacagctgc ttataaagca tttgaaaaat acaaaaaaga aaacggtggc ggcactaacg
    69301 caaagattct agtggctgat aactggcatg aagaaggtga acgtaattga tttacctgtt
    69361 cattttcgtg gtactaacta tttttatgct agtaatctta ttcaaaaaaa gatgagtaaa
    69421 gacgaacgaa aaattctggc caacttttat ttagtattaa aaatcgaaca aaatgtgttt
    69481 tcggaaagga gaattgtatg ggaaaaacaa aatcaaagat taagaagaaa aaacgacgtt
    69541 tggaacaaaa ggcaatccag aatggaacgg ctaagaaaaa ataaaaaagc tacccctttc
    69601 gagatagcag cgcaatacta ttctaccata aggggtggcg tttgtgagat ttcattggtt
    69661 aaaagattac caagaactag atgagcagat tctttactta aagtggaatc ttaataaaag
    69721 taagcttgaa ttgaatcgat gggtctgtgg tgatttagca aacgtccgca tcgaaaagaa
    69781 ttcgagaact tcttctttag aagagaatat ccaaaagata gaaaatgaat tggaattgtt
    69841 gatcgagcaa aaagaagaaa tgttattgct gatagatagc ttttctggta tcgataatca
    69901 aattgttaag atgaaatatg ttgatcaaat gagcttagaa gatattgctg aatctgttgg
    69961 gtatagctca tcgtatgtca gacagcgaca tgcagaaatt agaaagacat tgaacttttt
    70021 agatgagtat gaacacagac aagctgaccg attgaaaaaa gaaaacgaaa ttgattttta
    70081 caatagcgag aagtacaaag aacaactctc tttattctaa aattacactg ttcactaaat
    70141 gttcggtcat tgtagctatg taaacattgt tagcagcatg atattctatt agtgtcaaaa
    70201 aaatatgaaa gagccaagat atcccaaccg ttttattaat tggtatctgt ggctctttta
    70261 ttcttttaat tagtagttgg cataaagtta tttccattta taaattgatc agctaaatca
    70321 gcatcataat cagccgcgat aaattgcgct gctgatttaa taaatgcatc catatcatga
    70381 acatctttat tagtatgtct acgaacatag tgcgtttcat cattaccaat ccaagtagca
    70441 gctttggcta atctttggag tttaggaaaa tcattcaaat aattttcaat tgttttacct
    70501 aataacattt tttttatttt atcttcatcc tctgaatttt tagatattgc ataatcttta
    70561 attagaaatt ctagagcttt tctataacca actccagcaa tttgttccaa ttgctcagat
    70621 ttagcttgtg tggcttgagt ataaatatca acaaatgtag gagaaacttt ttcaatattt
    70681 tctggtaaat ctattttaat cggcggacgg taataatctt taattaaaat attttcagaa
    70741 tatccttcat cttcattaaa cgtatttata aattgtaaac tataaaattt gttgcactca
    70801 tcatttatac actgtactaa tacgccaaag attctatcat tatcgaacgc actatatgat
    70861 gaaaccccaa aatacacata cggagtcatt atatttccgc aatgcggaca actatttggc
    70921 aaataaattg tctctgtatt ttgacgatca cgatacttaa tattaacttc ttgaagcatt
    70981 aaaacacctc gcatcttttt tataaatcat accacaaaat atctgatagg agagtaaaca
    71041 aatgaatgat tttcatgaag cggtacttag tattgaagta gaatcaagtt tggctaaagt
    71101 ttataagaaa gcaattgaag cagagaatag tccgtacaga gaaaattgga atggtaatca
    71161 tgcacatgtg caagtggaga atgatgatta cagaactgga atgaatacgt tagttatttc
    71221 tttattgtca cacactctac ccaatctgca ggaaacgatt gaatggtatg agcgaatggg
    71281 cgcaaaagta atccgtacaa attacaaagg agagaattaa aatggcaatg atcaaaatta
    71341 gaacatccat tagtggaaca gagtattggg attcagaaaa gaaaaggaca gtagttgttc
    71401 cgaaagatca agaaccaaat tttgaagtaa aagaagaaaa aggaattttg agtgatggca
    71461 aatcatttgt tgctgtcaat ggcgaactca tagctcatag cagtgaaatt cttgatagcg
    71521 atggaaacac tgctgctaat tttgatggag acgaagctac taatgatcag tccactgaag
    71581 atacggatga actggacaat atgactgcaa aagaattgcg tgcatatgct aagaaacatg
    71641 gtattgatat tcctggtgct atccgtgcaa aaggcgatat cataaatctt attcgtgaag
    71701 tagaatgaaa tactgtcagt ttgacggatg cacgaacaaa atagcaaagg gtatctactg
    71761 tactgaacac aagagatcaa gcagatcacg caagaagaag cagcaagcaa agtctgttta
    71821 tcatcatgag aacaaaccat tctatcgaac gcaagcatgg aaagatatgc gtcaatttat
    71881 ttatgaaaga gaaggtggtc attgtcagcg gtgtggtcag ttcatatttg gaaagagagc
    71941 acacgtccat cacattgtac caatcaaaga caacgaactg cttaagcttg atccaaacaa
    72001 tctcatgcta ttatgttcaa aatgtcatcc aattgttgaa aacgaaacgg aagacaaaaa
    72061 agtttttcct tcgtatttca attgaagccc ccctattcat tttcaaaatt ttttcgcgtg
    72121 gggagatagg gtagcgggga gtcacgcgca tcgttaggtc aaatttttca aaaaacaaag
    72181 gggggtgtat acaaaaatga cgactaaagc gcaacgtaaa gcgattattg atgaaaaagt
    72241 aagtgctgaa aaagctcgta tcttagaaat aatgaatttg tctgatttgt acaccatcac
    72301 tcttgatcca ttaatcgaat catacttgga tatttttgaa atttaccaac acaaatattt
    72361 attgtggaag gaaaaaggat ttccggagac gcaaaaattc acgaataaat caggagctac
    72421 taatcaatca aaacatccat tggcacaaca agtagaaact tgggcagata agaagatgaa
    72481 agctctagat ttattaggat taacaaacaa agcaaaaact ggtagactaa ttgctggagg
    72541 atcgaccgca agaaaagatg aagaaattac acgcccagaa gtaaagccag tagatgaact
    72601 agcagcgcac agaaataaat ggcgtaagaa ggcaggtgct gaaaaatgat tgaacctggt
    72661 gtaaattatg ctgatttatt tgcaaaagaa gtaagaaaga aacctaagaa gtatcctaag
    72721 accgttcgtt tagcaataga tcgctggtat cagtggaaaa aaagaaaaga tatttggttt
    72781 gatgtagatc gcgcgaatga aatgatggat tgggtagaat catttattgt tcacacaaaa
    72841 ggagaaatgg tgggaaaacc gtttctttta gaaccctggg aaaaatttat ttattcttgg
    72901 atttatggtt gggttaaaga aaatgaaaaa ggccaaattg tacgcgttac tcgtgaggct
    72961 tatgtccaga ttccaaaaaa gaatggtaaa acattgattg ctgtcggttc attaggatat
    73021 gcaatgtacg gagaaggggc gttatcagtt gattgctatg cttgcgcatc tgattttgct
    73081 caagcgcaat atgctgccaa accttttgca gttacaatct taaacaatcc aatcctactt
    73141 gaaggaacta aaatatttag ggggccaaag ggaactgttt ctagtattac ttatgactat
    73201 attcatgaag atatggctta ctcaaataaa tttattgttc aaaccaagaa tattgataat
    73261 atcgagggat ccaatccata ttttgtattg aacgatgaat tgcataaaca ggagaaaatg
    73321 gaacagtatg acaattttaa gtctgctcag atatctttac cacagccatt gatgtttaat
    73381 atctcaactg ctgggaaagg ttcgtcgtct gttggaatgc gtgtatatcg cgaagcaaaa
    73441 gaagtgttga aacgtgatga taatgattca aactttgttc taatctatga gccaaataaa
    73501 aattatgatt ggacggatag aaaagtctgg gaaatgtgca atcctaactg gggaatatca
    73561 gtcgatttat ctgctttaga atcggcattt aaaacagcgc aacggtcagc tcattccaaa
    73621 gctgaatttc taacgaaaca cttggatgta tttgtgaacg gcgcggataa tttctttgaa
    73681 caggatcaag tagagccatg tttagttacc acacaagaac ttggtgattt aagtggcgaa
    73741 ccatgttata tcggtttaga tttatcacgt acacgagatt tgacctgtgt atctttaaac
    73801 ttcccaacat gggatgaaga tggtaaagcg gttcttaaag taaagcaact ttattttatt
    73861 ccgaatgaag atttagagtt tcgagaaaaa gaagataatg ttccttacag tgatttggct
    73921 gaacaaggtt tcgttgaatt ttgcgacgga aaaatgattg atcaagatca gattttgcag
    73981 tacatagagg actgcatgaa tttatatgat attcagcaag tgaattatga tccagcgatg
    74041 agtgacaaac ttgttgagaa attagaaaac ttaggattgg aatgcgttga agttgctcaa
    74101 tatcctaaag tattgaatgc tccttttgac gatgtcgaac ggttgtttta tgagaaacga
    74161 attcaatttg ataatccatt attcctttat tgcactttga acgttgtagc aattactaac
    74221 atcaatggac aaaaagcgcc aagtaaacgt caatcaaaga aaaagattga tgggttcgtg
    74281 gcgtttttgt gtggtcataa ggaaacgatg aatcaaatga cagatattga ttcagatgag
    74341 ttagatgatt acctaagttc catctataga taaatagaaa ggcggtgaaa aaaattgaaa
    74401 ttacgtgatc ggttatcgaa tgcagtctat tcttttatgg aaaaacgtgg atatatcgag
    74461 gatatttttg gacactacac acgttatggt caaagatatg tgacggattc atctatcatg
    74521 gaatcttctg atgtttatga attagttcaa gacatatcta atcaggtggc attggccaca
    74581 ccaattgtta ttggtcccga cggcaacgaa gtcaaagatc atcacttact aaatattttg
    74641 aagagtccca atgattattt gacaggattc gaatttacca aattggaaac aaatacttta
    74701 ttgatcaatg gtgagacatt cccattaacg gatagggatc agcttcattt ggcgtacggt
    74761 gtaacaacta aaatcaatga acgacttcaa gaagaatttg aaatgaatgg tcaaaaaata
    74821 cctggtcaaa tgattcgaca cattaagaac atcggaactg attcattaaa aggtgctgga
    74881 ataattgatc ttgcaagaaa cactctagaa ggcgttctga gcgctgaaaa agttttgacg
    74941 gacaaatata ctaaaggcgg tttacttgcg ttcatgctta aactagacgc ccacatcaat
    75001 ccaaataata gcgcccaaac aaaaattgtc aaagctatat tggatcaact ggaaggaacg
    75061 caaaatgaga gtgatcattc tgttaagatg attcctttgg gaaaaggata ttccatcgag
    75121 acattaaaaa gtcctgttga tgacgcggca attttgaatt atttaggtgt ttacaaaaaa
    75181 gacttaggaa aatttctagg aatcaatgtt gatacgtatc aatcgctgat gaagacagat
    75241 attgaaaaag cgatgatgta tctgcacaac aaagcaatca aaccaatatt gaaaaacaag
    75301 agcgaacatt acaccgctct tttttttatg cctaattctg gctatagagt ggaatggaaa
    75361 attaatattt tggattttgt tccttactca acaaaaacaa atattggcta caacatcgtt
    75421 cgtaccggga ttacaagtcc agataacgtg gcagaaatgc ttggttttcc taaacagaat
    75481 actccagaaa cacaagctat ctatatttca aatgacttat ctaggattgg ccagaaaaat
    75541 gcaacagatg attccttacc aacgaatgat caagacttga aaggaggtga tggaaatgaa
    75601 gaagaaggaa attcgcacga ttgacatcac caatctttca acacgttccg atgaagaaac
    75661 tcatacgagg accattagcg gctacgctgc tgtattcaac agtcaaacac ttttatggga
    75721 tgacttaagc gaagtgattg ttcctggtgc attctcaaag gcaattagta attcggatgt
    75781 tcgttgtttg ttcaatcacg attggtccaa tgtgctagga cgtactaaaa gcggaaccct
    75841 tcgccttgaa gaagatgaac gtggtttgaa attcgaagtt gatttaccgg acacaacggt
    75901 agcaagggac ttagttaaat ctatggaacg aggagacatc aaccaatgta gttttggttt
    75961 tgtgccgact gaagaaacgt gggactacaa ttctgaacca atgcttcgaa caatccatga
    76021 agtggaatta tacgaggttt ctattgtgcc tttgccagca tacgaagata cagaagctgc
    76081 attaagaagt cgtgatgaat tagaaaaaaa cgtcgaagaa agaaaaaaat taatcaaaaa
    76141 aattaatcaa gcgctagaag cgtaggagga aaaatacatg gataaagaat tattgaaaaa
    76201 aatgaaggca cgtcgtgagc aacgattgac tgaattacgt gaaaaagttg aatcaggaga
    76261 attacgcgaa gcagatttag aagccgtgaa agaagaaatt gacagtgtta ttgatgaatt
    76321 gaacggaatt aaagacgaat taggcgcaga ttctggaact gatgaaacag acgacaatac
    76381 agatgatcaa tcgaatagta cggattcaga cgaaagccgt tctggtgaag acaacgatca
    76441 agaagaagat tcagacagtg aagatagttc agaaaatcgt tctggaatga ttactcaaca
    76501 gcaacgagat ggattacttg gatcaattaa gaacggattg gaggcacgtg caaaaatgac
    76561 caagaaacaa aaagatcaac aactacgaaa agcatttgct aattttgtag ctggaaatat
    76621 ttctgaagca gaagctcgag ctttagggat tgaagctggc aacggttcag ttactgtccc
    76681 agaagtaatt gcatctgaag ttattactta tgctcaagaa gaaaatttac ttcgtaaata
    76741 cggaacagtg gtgcgaacat caggagatgt caaatatcca attcttgtga agaaagcaga
    76801 tgcgaatgta aacaagaaag agcgttcaac tgatattgct gaaacagcta ttcagtttga
    76861 tgaaattttg cttaatcctg ccgaattcga tgctttggca acagtaacta aaaaattatt
    76921 aaaaatgtct ggtgttccag ttgaagatat tgttgtggaa gaattgaaaa aagcttatgt
    76981 gcgtaaagaa atcaattata tgttcaatgg tgatgacgct ggaaatgaaa atcctggtgc
    77041 attagccaaa aaggctgtag catttaaaaa acctttagat ctaactgctt caggtgctgg
    77101 gcaaaaatta tatgatgcat taatcgaatt taaaaataca ccagtgacag aagtgatgaa
    77161 aaagggacgc tttattatta atcgagctgc tttgactgcc attgaaaaaa tgaaaacaga
    77221 tgatggattt cctttgttgc ggccattcac acaagcagaa ggtggaattg gttaccaatt
    77281 agttggctat cctgtggatt ggacagatgc agcagataaa aagggtgaac cagacacgcc
    77341 agttttatat tttggcgatt tttctgcatt taaaattcaa gaagttattg gtgccttgga
    77401 aattcaaaaa cttgttgaaa aattctctgg taaaaatcaa attggattcc aaatttacaa
    77461 cttgctagat ggtcaattgg tttattctcc atttgaaccg gcagtatatc gctacgaaat
    77521 tacaaaacca gttggtggtt aagatggaag agcaaactaa agaattgtct ttagaggaaa
    77581 aattcaaatc acatattcat tttgaagagg gcatggatga ttctttgctc tctttttatt
    77641 taaatatggc aaaagattat gtcaaaacag caactggtgg ccaacaagaa tatcttattt
    77701 tgatggttgc cggcattgcc tatgaatata gagtttcaga ggatgaactc gacaaagcta
    77761 tgaatgccat gacgccattt atcgtgcaag gagcgattca aaatgccgaa gagacagact
    77821 aataatctca gatggaaagc cgaattgcta gacatcaaaa caggaacaga cggaaacgat
    77881 cgtccaacta caatttacga atttaagcgt ctaatattct atgaagaact cggtgtgact
    77941 tctcaagaaa aatatttatc acagcaagcc aagacagacg ttgtcagacg aattaaagtc
    78001 agatgggata aatccatcac agagaaatta agtgcgctca aaattgattc tgtaacgtat
    78061 aacattactc gcatttatac gaatcccgat gcaagagaaa tggagttgag tttagcttat
    78121 gtcgattagc tttgatgaat tgaaaacagc gctgaaatca acaaagttag cagtgttcag
    78181 agacaaagcc agattaggga cgatgtatcc atacattgtg tactcaaatg tgagtaacag
    78241 caaaaaaatg gcatccggta aagtctataa aaaattacct tattaccaag tctctttttt
    78301 tacacttgga acagaacagg atttagttgt tattgaacaa gcgctgcaaa atgctggcat
    78361 tccatattca gattttacag gtatacaagg tgatgagaac gacgataccg taaccaacta
    78421 ttacacatat gtgaggtgtg tggaaaatgc caagtaataa gaatggtttt tctgaaatat
    78481 cggattattt aggaaacctt tcaaaagttg atccgaaaaa attatcgttg gaatctttag
    78541 aagaagcagc taacttttat ctaaagcagt tgcttcctaa tattcctaag tcgctcttaa
    78601 aaaagaaaca tatgcgcgat caaataaaag tagtagttga aaacgatcaa gtgaaagttc
    78661 aatttgaaga gacggccttt tactggcgct ttgccgaaaa tggcacaaca aatcaacgtg
    78721 cccaacattt tgcaagtggt acgtatgaac aaaacaaaga aaaaattgaa gaaattatga
    78781 ctagaaaaat acttgattta tgggaagggt gattttaatt gggaaaacaa gatacttttt
    78841 attttgaagg attggacgac attttaattg cgatgatggc aacgccagat tctgttggaa
    78901 ctgcaccgac ttatagtgaa gttttacgat tgccaattgc cacaaaaatc ggtgtcaaag
    78961 ggaatggaac agcattagaa aaatgggcat ccagcaaaat gtttcgtcgt gtgtcgcgag
    79021 aaacaaaaca tgaacttgcg ttggatcacg tgggtattcc gatagaagtg atggatgaaa
    79081 tcaaaggatt agttgctaaa agcggagtaa cgtttagcaa aaataccgca cgagaatttc
    79141 cgtacttcgc ctttggattt attggaaata ttgaaaatgg cggtaaaaaa gcagtttggt
    79201 atcctaatac acaactatcc aatgtgattg acgaagagta tgccactgca gaggaagaaa
    79261 caaaaattga cgatgtaact gcaaatctgg tttccatcgg actgaaatac aataatgtga
    79321 tgtatgcaag ttttgattca aatagagacg gagcttctct agaattattt aataaattca
    79381 ttgctcaacc tgtatacgat gaagaacagt ggaaaacatt ggctaaagtt ggaggtgcgg
    79441 gttaatggct cggttatctg attatggaat tcacgttgaa gacttaaaaa attctgctgt
    79501 tgtaattatt caaggcgcag aattccctat ttcatttact atgcaaacga tggaatttat
    79561 agcagatgtg tatggtggag attattcgca atttgaatct gatatgaacg ccatgctatc
    79621 taaaaaagaa ggacaaattt cttctgctaa cttatcgcct agtgatctaa aaatcatgcg
    79681 tgccttgatt tatgccatgc tgcgtactgg tggtttagag gaagatccag aaactatttt
    79741 caaatttttg ggaatgagtg gagaggtgtt gtctgcttat agtacctgta tggaaatttt
    79801 tgctagccag acatttcagg tggaagacct aaaaaaatcc aagaagccac aagactttca
    79861 aaaagcgcaa gcaaaaagaa agaaaaacaa aaagaatcgg aagagatagg aactccttgg
    79921 agtttttata tttacgttgc tctcactcta ttgaattgga gtgagagttt ctttttaaag
    79981 tctacaccta acttgtggct caaatcttat ttacagtggt tacaacaaaa caccgatttt
    80041 gaaccacctc aatctgtaac tatggataaa tcgccttggt ggtagaaagg agcgctaaga
    80101 taaatggctg gtaaagaatc tgatgtcgtt cttaatttta aaacgaatgg cgaagtcagt
    80161 tattcgaaaa caatcaaaga aatcaacaaa gaaatgaact tagccgctgc cgagtacaaa
    80221 aaccaagtgt ctgcgatgga caaggatgca actcaaacag aaaaattgcg agcggctaaa
    80281 caaaaattag aaaaacaatt aggattggct gaacaacgat ctcaaatgct gagagaggaa
    80341 tatgaaaaat ctgtcaaaga aaccggtgaa tattctgctg aatcagaaaa actttataaa
    80401 caattactca attctgaaac cggagaaaat aaacttcgta cagcattaga acagacgaac
    80461 gatgcactta aagaacaagg tgacgtttct gttgatacag caaaaaaact ccagaaaatc
    80521 gaagaaacag gtgagaaagt aaaaggcgtt ggtgaaaaaa tgtctgttgg agtaaccgcg
    80581 cctattgtag cggcaggagc agcaggactt gcagcatttg gtgaagttga cgaggcactt
    80641 gataccatca ttacaaaaac cggagcaaca ggtgatcaag ctgatagact ttcacagtct
    80701 ttcaaaaacg ttggttcaaa tactcattta cctttacaaa cggttgggga agctattggt
    80761 gaggtaaata cacaatttgg attcatggat aaaaaactgg aagattcaac caattatctc
    80821 ctacagtacg ctgaaatcaa tgatacagat gtttcgcaat cagcaatatc tgctcgacaa
    80881 gctattgatg cttatggact agaatatgat gatttgaatt ctgtccttga tgtaacaacg
    80941 aaaacatcgc agaatactgg tcaatctgta gacgacttga tgcaaaaagc aattgatggc
    81001 gcaccacaaa ttaaacaatt agggttgagc tttggtgaag gggtcacttt acttggacaa
    81061 tttgagcaat ctggtgttga ttcaagcgca gctttaagta gtttatctaa agcaacagtg
    81121 gcttatgcga aagacggaaa atctttaagt caaggactag aagaactaca agacaaggta
    81181 aaaaatgcag gttctgaaac agaagccatc aatgcagcag cagaagtatt cgggactaaa
    81241 ggcggtccta gaatggccga tgcaattcgt agaggtactt tgaacctgga agatttagca
    81301 aaaactgctg gagaaagtgg gggagctgta ggagacacat tcgatgctac tctcgaccca
    81361 attgatcaag ccgatcaagc tatgaacaat gcaaaattag caatggctga cgtaggtgaa
    81421 gcagtccaag taagcctttt gccttttttt gaaaaagcaa cttccttatt acaggaattc
    81481 tctaaatggt gggggtctct agatcaagat acaaagaatt ggatcataac aattgctggt
    81541 atagcggcag cagttggacc agtactaatc gttcttggtt cacttatggg atctgttaca
    81601 aaaatcgttg gggaagttaa aagttttata ggagtttggc aaggacttgc tggtttattt
    81661 ggaatgtcgg gaggctggtt tgcattagca gttatagcta ttggcgcatt agttactgga
    81721 ctaatttggg catataataa cgttaaatgg tttcatgatg gagtgaattc tttcttcaaa
    81781 ggtgtttcag atgtagctgt tgaaatattt aattttttaa gtggttattt tggtggattt
    81841 ttcgatggtg ttatagcaaa tttcaataat ttttataatg ctggaaaacg aattttcagt
    81901 ggttttctag attttatcac tggagtattt acagggaatt ggtcaaaagc atggcaaggt
    81961 ctagttgata tttttggagg tattttcgat ggtattgttg ctgtagggaa agctcctatt
    82021 aatgccatga ttggattgat caatggattt attggtggat tgaataatat aaagatacca
    82081 aaatgggttc ctggtgtagg tggaaaatca ttttctattt caaaactgcc ttatttagca
    82141 caaggcggtc atctaatcaa tggacaagcg attgttggtg aagctggacc tgagttactg
    82201 actgccaaga atggtaaaac cacagttact ccattatcag acgaagaaaa acgcaaaggg
    82261 atcagtggaa gagtttctgg aggtagcatt gaacagcata tacacattgg caaagtcgat
    82321 gcaaacaatc catctgagtt agatcgtatg aatcgtaaat ttgctaaagc aaacaaacag
    82381 gctattcgtg atttgggagg tgttcctatt tgagtcgaca atttatgaat ccagatgaac
    82441 caaattttat ttggaaaaat cggaatgcag taattgatat gaattgcatc attgaggcag
    82501 agcttccaga tattatgccg aacaagcgat acgaaactta cacgattcag ggccgaagcg
    82561 gagaattgac ggagacgttt gatgactatg aaccttttga tttggaaata gaagggatca
    82621 ctattccaca ttcgaaactg agagaagtca aacgatggct cactggtaaa agccaattaa
    82681 tcacacataa tgatccagac aagtatctag aggctatctg cagcatggat aaagaagtcc
    82741 ctttcgaaaa cgagtggggc ttcttttata cattcgatgt tacttttcgt tgtcagccac
    82801 tcaaaagaaa aatcggtgaa gcacctaaaa tttttcattc tagtgttatg gatatttttg
    82861 atcatggtga cgaaattgca catccctatc tggaaataga atcgaacgga ggagatataa
    82921 cgatcgctgt cggtgataaa agcctaacaa tacttaatac gttagctgca acaatcgtgg
    82981 ttgatactca gcttggaaaa gcaatacaag aaggtttgaa tttatttacg cgtggtgatt
    83041 ggccagtatt gcagcctgaa tggaatcagg taaaaatatc cggaaaattt aaagaagtgc
    83101 ggttttggaa taggagtgtg tatctgtgac gcaagaattt atttatgctt ataaaaaaat
    83161 gccagatgat ttgagcgtga acggagcgtc gttagttgat tgggaagatt tgccagaaat
    83221 caatcgtgtg ctgaatggcc aatatcgttt ttatggtaac tattcaagaa gcggacagta
    83281 tcgctcttat ttaaaaaaag gaaattttat aaaagcgaag gtaccagatg gatcatggca
    83341 atattttgaa atttataatg tcaaaaagaa tttgacctcc gtttctgtaa cagctcggca
    83401 tattggtttt atggccaata agaactttat tgtcaagtcg tttacggata acggcaatgg
    83461 gtctcagatc atgaccaatc ttaaaaacag tttggcattt gatcaaaaat ttaactatct
    83521 ttcgaatgta gggacaacgc accaatttac agcaagacaa gtcgcgccag ttgaagcaat
    83581 cattggttca aataacggta atcaaaattt gactggcgtg actagcgcgg aacttgatat
    83641 ggataactat gatttgaaac tggtaaaaca gattggatca gataatggtt ttcggattga
    83701 ttttggcatc aatttagaag caatcgaaga agaaattgat gaagaatcaa ttgtcaatag
    83761 tttgtatttg gttggcggtg ttcctgataa cgattatgat gaagacaaag aacctatcga
    83821 atatggttat ttagaaattg atggagtaac caacgagaat cgacgtattg ccaaacgtga
    83881 aaacggagat tgtaaaacag ttgatgaatt gatcaaatgg ggcaaaacct tatttgacaa
    83941 tgatcgtatc catgagccaa aagctactca tacagtcagt atggtggcgt tggaacacac
    84001 actcgaatac ggtgacatgt atcaagagct agcgtcttta cactttggtg atgtcgctca
    84061 tgttagggcg aaagagctag atatcgagat aaaagagcgt atggtcgaat acacttattt
    84121 cccaacgctt gggaaatata aggatattgt tttaggtaat gatttatcac tgtacacttc
    84181 ggcagtaaat actcaagcac aagaattgaa aaagaaaatc gacaatcgaa cagaaacgtt
    84241 agttcaaaat gtgttgaatg cgacggcatg gatcactggt aattctggtg ggcatgtcgt
    84301 ttttcgtcca gaaaaagctc catcagagat tcttatcatg gatactccaa atgtggcgaa
    84361 tgcaaagcga gtgtggcgtt ggaatttaaa tggcttaggt tattcagaca acggagtaaa
    84421 tggcccgttc ggtattgcta tcacatcaaa aggagaaatt gttgctgact tcatcaaagt
    84481 aggaacaata aatgcagaag tattcgaaac ttcctttaat gcttatggtg atgtgttaaa
    84541 acttgtaaaa ggcacgcttc aaatttggaa tgaaaacaaa aaaatcatgg agctaaccaa
    84601 aaaaggtatg gaattctgga atagaaaaga atccattgga acgatcggaa caacagattc
    84661 agcaggaaat ccatttccaa atgctgtaac acctacacct cttgaggaaa attctttggt
    84721 aatacgtaca aatggcgatg gaaaatacat tttaatttct ccaactgcag aaaaaggatt
    84781 tgtgttatta ggaaacggaa aagcatatta tttcggtgat ttagatattc agggaaaact
    84841 tactgttaga ggtaaagaag tgataccagg acaaaatggc ggtccatctg gcggaggaga
    84901 aacacctggt gggtatcctg atgaattaaa aacagatgca gaaaagagag cttggagaat
//
LOCUS       DAZUJO010000004        65313 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000004 DAZUJO010000000
VERSION     DAZUJO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.004"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8OL_000292"
     CDS             476..1339
                     /locus_tag="ACG8OL_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4816308.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8OL_000293"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8OL_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4816309.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8OL_000294"
     CDS             3339..3860
                     /locus_tag="ACG8OL_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4816310.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8OL_000295"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8OL_000295"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS4816311.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8OL_000296"
     CDS             5941..6759
                     /locus_tag="ACG8OL_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS4816312.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8OL_000297"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8OL_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816313.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8OL_000298"
     CDS             7228..7806
                     /locus_tag="ACG8OL_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4816314.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8OL_000299"
     CDS             7901..8038
                     /locus_tag="ACG8OL_000299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816315.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACG8OL_000300"
     CDS             complement(8851..9354)
                     /locus_tag="ACG8OL_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4816316.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACG8OL_000301"
     CDS             9713..10738
                     /locus_tag="ACG8OL_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4816317.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACG8OL_000302"
     CDS             11061..11939
                     /locus_tag="ACG8OL_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS4816318.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12415..12531
                     /locus_tag="ACG8OL_000303"
     CDS             12415..12531
                     /locus_tag="ACG8OL_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4816319.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACG8OL_000304"
     CDS             13459..13797
                     /locus_tag="ACG8OL_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816320.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACG8OL_000305"
     CDS             13891..14121
                     /locus_tag="ACG8OL_000305"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS4816321.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="ACG8OL_000306"
     CDS             14521..14892
                     /locus_tag="ACG8OL_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS4816322.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="ACG8OL_000307"
     CDS             complement(15166..15858)
                     /locus_tag="ACG8OL_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4816323.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="ACG8OL_000308"
     CDS             complement(15861..16646)
                     /locus_tag="ACG8OL_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4816324.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8OL_000309"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="ACG8OL_000309"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS4816325.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="ACG8OL_000310"
     CDS             18308..18625
                     /locus_tag="ACG8OL_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4816326.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="ACG8OL_000311"
     CDS             18638..20311
                     /locus_tag="ACG8OL_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS4816327.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="ACG8OL_000312"
     CDS             20325..21956
                     /locus_tag="ACG8OL_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS4816328.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACG8OL_000313"
     CDS             complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACG8OL_000313"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS4816329.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACG8OL_000314"
     CDS             complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACG8OL_000314"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS4816330.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25190..25795
                     /locus_tag="ACG8OL_000315"
     CDS             25190..25795
                     /locus_tag="ACG8OL_000315"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4816331.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26103..27119
                     /locus_tag="ACG8OL_000316"
     CDS             26103..27119
                     /locus_tag="ACG8OL_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4816332.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27260..28561
                     /locus_tag="ACG8OL_000317"
     CDS             27260..28561
                     /locus_tag="ACG8OL_000317"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS4816333.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28607..29269
                     /gene="deoC"
                     /locus_tag="ACG8OL_000318"
     CDS             28607..29269
                     /gene="deoC"
                     /locus_tag="ACG8OL_000318"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS4816334.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29290..29688
                     /locus_tag="ACG8OL_000319"
     CDS             29290..29688
                     /locus_tag="ACG8OL_000319"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS4816335.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29754..30836
                     /locus_tag="ACG8OL_000320"
     CDS             29754..30836
                     /locus_tag="ACG8OL_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4816336.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31048..32610
                     /locus_tag="ACG8OL_000321"
     CDS             31048..32610
                     /locus_tag="ACG8OL_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4816337.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32603..33736
                     /locus_tag="ACG8OL_000322"
     CDS             32603..33736
                     /locus_tag="ACG8OL_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4816338.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33736..34692
                     /locus_tag="ACG8OL_000323"
     CDS             33736..34692
                     /locus_tag="ACG8OL_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4816339.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34791..35957
                     /gene="deoB"
                     /locus_tag="ACG8OL_000324"
     CDS             34791..35957
                     /gene="deoB"
                     /locus_tag="ACG8OL_000324"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS4816340.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35981..36799
                     /locus_tag="ACG8OL_000325"
     CDS             35981..36799
                     /locus_tag="ACG8OL_000325"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4816341.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36816..37523
                     /gene="deoD"
                     /locus_tag="ACG8OL_000326"
     CDS             36816..37523
                     /gene="deoD"
                     /locus_tag="ACG8OL_000326"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS4816342.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACG8OL_000327"
     CDS             complement(37821..38507)
                     /gene="gpmA"
                     /locus_tag="ACG8OL_000327"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4816343.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38679..39260)
                     /locus_tag="ACG8OL_000328"
     CDS             complement(38679..39260)
                     /locus_tag="ACG8OL_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4816344.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACG8OL_000329"
     CDS             complement(39393..40076)
                     /gene="rpiA"
                     /locus_tag="ACG8OL_000329"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS4816345.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40395..40808
                     /gene="rpsL"
                     /locus_tag="ACG8OL_000330"
     CDS             40395..40808
                     /gene="rpsL"
                     /locus_tag="ACG8OL_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS4816346.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40892..41362
                     /gene="rpsG"
                     /locus_tag="ACG8OL_000331"
     CDS             40892..41362
                     /gene="rpsG"
                     /locus_tag="ACG8OL_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS4816347.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41456..43543
                     /gene="fusA"
                     /locus_tag="ACG8OL_000332"
     CDS             41456..43543
                     /gene="fusA"
                     /locus_tag="ACG8OL_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS4816348.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43715..44902
                     /gene="tuf"
                     /locus_tag="ACG8OL_000333"
     CDS             43715..44902
                     /gene="tuf"
                     /locus_tag="ACG8OL_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4816349.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACG8OL_000334"
     CDS             45309..45617
                     /gene="rpsJ"
                     /locus_tag="ACG8OL_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS4816350.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45648..46277
                     /gene="rplC"
                     /locus_tag="ACG8OL_000335"
     CDS             45648..46277
                     /gene="rplC"
                     /locus_tag="ACG8OL_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS4816351.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46305..46928
                     /gene="rplD"
                     /locus_tag="ACG8OL_000336"
     CDS             46305..46928
                     /gene="rplD"
                     /locus_tag="ACG8OL_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS4816352.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46928..47218
                     /gene="rplW"
                     /locus_tag="ACG8OL_000337"
     CDS             46928..47218
                     /gene="rplW"
                     /locus_tag="ACG8OL_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS4816353.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47257..48090
                     /gene="rplB"
                     /locus_tag="ACG8OL_000338"
     CDS             47257..48090
                     /gene="rplB"
                     /locus_tag="ACG8OL_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS4816354.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            48133..48411
                     /gene="rpsS"
                     /locus_tag="ACG8OL_000339"
     CDS             48133..48411
                     /gene="rpsS"
                     /locus_tag="ACG8OL_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS4816355.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48434..48781
                     /gene="rplV"
                     /locus_tag="ACG8OL_000340"
     CDS             48434..48781
                     /gene="rplV"
                     /locus_tag="ACG8OL_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS4816356.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48795..49451
                     /gene="rpsC"
                     /locus_tag="ACG8OL_000341"
     CDS             48795..49451
                     /gene="rpsC"
                     /locus_tag="ACG8OL_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS4816357.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49454..49888
                     /gene="rplP"
                     /locus_tag="ACG8OL_000342"
     CDS             49454..49888
                     /gene="rplP"
                     /locus_tag="ACG8OL_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS4816358.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49878..50066
                     /gene="rpmC"
                     /locus_tag="ACG8OL_000343"
     CDS             49878..50066
                     /gene="rpmC"
                     /locus_tag="ACG8OL_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS4816359.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACG8OL_000344"
     CDS             50091..50357
                     /gene="rpsQ"
                     /locus_tag="ACG8OL_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS4816360.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50415..50783
                     /gene="rplN"
                     /locus_tag="ACG8OL_000345"
     CDS             50415..50783
                     /gene="rplN"
                     /locus_tag="ACG8OL_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS4816361.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50820..51128
                     /gene="rplX"
                     /locus_tag="ACG8OL_000346"
     CDS             50820..51128
                     /gene="rplX"
                     /locus_tag="ACG8OL_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS4816362.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            51156..51695
                     /gene="rplE"
                     /locus_tag="ACG8OL_000347"
     CDS             51156..51695
                     /gene="rplE"
                     /locus_tag="ACG8OL_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS4816363.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51714..51899
                     /locus_tag="ACG8OL_000348"
     CDS             51714..51899
                     /locus_tag="ACG8OL_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS4816364.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51935..52333
                     /gene="rpsH"
                     /locus_tag="ACG8OL_000349"
     CDS             51935..52333
                     /gene="rpsH"
                     /locus_tag="ACG8OL_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS4816365.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52365..52901
                     /gene="rplF"
                     /locus_tag="ACG8OL_000350"
     CDS             52365..52901
                     /gene="rplF"
                     /locus_tag="ACG8OL_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS4816366.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53062..53418
                     /gene="rplR"
                     /locus_tag="ACG8OL_000351"
     CDS             53062..53418
                     /gene="rplR"
                     /locus_tag="ACG8OL_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS4816367.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53439..53939
                     /gene="rpsE"
                     /locus_tag="ACG8OL_000352"
     CDS             53439..53939
                     /gene="rpsE"
                     /locus_tag="ACG8OL_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS4816368.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53954..54133
                     /gene="rpmD"
                     /locus_tag="ACG8OL_000353"
     CDS             53954..54133
                     /gene="rpmD"
                     /locus_tag="ACG8OL_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS4816369.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            54177..54617
                     /gene="rplO"
                     /locus_tag="ACG8OL_000354"
     CDS             54177..54617
                     /gene="rplO"
                     /locus_tag="ACG8OL_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS4816370.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54617..55912
                     /gene="secY"
                     /locus_tag="ACG8OL_000355"
     CDS             54617..55912
                     /gene="secY"
                     /locus_tag="ACG8OL_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS4816371.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55974..56621
                     /locus_tag="ACG8OL_000356"
     CDS             55974..56621
                     /locus_tag="ACG8OL_000356"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS4816372.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56812..57030
                     /gene="infA"
                     /locus_tag="ACG8OL_000357"
     CDS             56812..57030
                     /gene="infA"
                     /locus_tag="ACG8OL_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS4816373.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACG8OL_000358"
     CDS             57063..57179
                     /gene="rpmJ"
                     /locus_tag="ACG8OL_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS4816374.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            57197..57562
                     /gene="rpsM"
                     /locus_tag="ACG8OL_000359"
     CDS             57197..57562
                     /gene="rpsM"
                     /locus_tag="ACG8OL_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS4816375.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57590..57979
                     /gene="rpsK"
                     /locus_tag="ACG8OL_000360"
     CDS             57590..57979
                     /gene="rpsK"
                     /locus_tag="ACG8OL_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS4816376.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58059..58997
                     /locus_tag="ACG8OL_000361"
     CDS             58059..58997
                     /locus_tag="ACG8OL_000361"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS4816377.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59025..59405
                     /gene="rplQ"
                     /locus_tag="ACG8OL_000362"
     CDS             59025..59405
                     /gene="rplQ"
                     /locus_tag="ACG8OL_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS4816378.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59677..60516
                     /locus_tag="ACG8OL_000363"
     CDS             59677..60516
                     /locus_tag="ACG8OL_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4816379.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60492..61361
                     /locus_tag="ACG8OL_000364"
     CDS             60492..61361
                     /locus_tag="ACG8OL_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4816380.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61351..62148
                     /locus_tag="ACG8OL_000365"
     CDS             61351..62148
                     /locus_tag="ACG8OL_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS4816381.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62351..63097
                     /gene="truA"
                     /locus_tag="ACG8OL_000366"
     CDS             62351..63097
                     /gene="truA"
                     /locus_tag="ACG8OL_000366"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4816382.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            63761..63955
                     /locus_tag="ACG8OL_000367"
                     /pseudo
     CDS             63761..63955
                     /locus_tag="ACG8OL_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(64143..64919)
                     /locus_tag="ACG8OL_000368"
     CDS             complement(64143..64919)
                     /locus_tag="ACG8OL_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4816383.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            65042..>65313
                     /locus_tag="ACG8OL_000369"
     CDS             65042..>65313
                     /locus_tag="ACG8OL_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFS4816384.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKF"
BASE COUNT        20729 a        11664 c        13788 g        19132 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacat cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgcgatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg
    22501 gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg
    22561 gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt
    22621 gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt
    22681 gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc
    22741 atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc
    22801 aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt
    22861 ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg
    22921 catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt
    22981 accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc
    23041 accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt
    23101 acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt
    23161 gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc
    23221 gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt
    23281 ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt
    23341 tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt
    23401 ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta
    23461 ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg
    23521 gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt
    23581 agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa
    23641 gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg
    23701 ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac
    23761 aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact
    23821 gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt
    23881 aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa
    23941 ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc
    24001 aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta
    24061 ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat
    24121 ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat
    24181 tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa
    24241 aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat
    24301 ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa
    24361 agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga
    24421 tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat
    24481 gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga
    24541 tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt
    24601 ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt
    24661 tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta
    24721 cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac
    24781 ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag
    24841 gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat
    24901 ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc
    24961 ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa
    25021 tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct
    25081 ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata
    25141 cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca
    25201 ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa
    25261 aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta
    25321 tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat
    25381 cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg
    25441 gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa
    25501 gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga
    25561 agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca
    25621 tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat
    25681 ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc
    25741 agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc
    25801 tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag
    25861 ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata
    25921 gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa
    25981 gactgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc
    26041 tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata
    26101 ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct
    26161 cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt
    26221 cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac
    26281 attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga
    26341 tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc
    26401 gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg
    26461 gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt
    26521 atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc
    26581 ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc
    26641 atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca
    26701 acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac
    26761 atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag
    26821 atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg
    26881 atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc
    26941 tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt gatattggat
    27001 tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca
    27061 aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac
    27121 tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt
    27181 agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga
    27241 agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa
    27301 cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc
    27361 agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga
    27421 aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct
    27481 tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat
    27541 tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct
    27601 aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct
    27661 ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc
    27721 aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt
    27781 tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga
    27841 tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc
    27901 aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc
    27961 ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt
    28021 cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc
    28081 cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac
    28141 gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca
    28201 aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta
    28261 cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt
    28321 aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca
    28381 cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact
    28441 attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa
    28501 tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata
    28561 aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg
    28621 aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat
    28681 cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct
    28741 agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct
    28801 aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc
    28861 agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt
    28921 ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat
    28981 tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc
    29041 tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga
    29101 ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg
    29161 tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac
    29221 aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag
    29281 gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat
    29341 aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc
    29401 aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag
    29461 cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta
    29521 gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa
    29581 ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg
    29641 accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt
    29701 cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa
    29761 aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat
    29821 gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg
    29881 cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg
    29941 aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat
    30001 atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg
    30061 gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa
    30121 caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg
    30181 ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt
    30241 atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc
    30301 gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa
    30361 tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg
    30421 ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta
    30481 acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta
    30541 tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa
    30601 acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa
    30661 aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg
    30721 gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa
    30781 aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc
    30841 atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat
    30901 tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa
    30961 tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga
    31021 tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag
    31081 aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag
    31141 gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac
    31201 gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta
    31261 aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt
    31321 atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc
    31381 gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat
    31441 ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg
    31501 gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca
    31561 gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa
    31621 gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac
    31681 cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc
    31741 tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa
    31801 gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt
    31861 ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc
    31921 gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag
    31981 gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa
    32041 atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta
    32101 gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa
    32161 aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat
    32221 gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa
    32281 aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca
    32341 actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg
    32401 gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg
    32461 gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca
    32521 tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag
    32581 ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt
    32641 gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg
    32701 ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg
    32761 gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt
    32821 agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat
    32881 cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt
    32941 atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg
    33001 agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata
    33061 cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc
    33121 ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg
    33181 cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct
    33241 gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga atccttttgc
    33301 ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg
    33361 cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt
    33421 tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg
    33481 atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact
    33541 tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat
    33601 tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg
    33661 gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga
    33721 agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg
    33781 gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg
    33841 atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc
    33901 aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt
    33961 ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct
    34021 gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg
    34081 atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt
    34141 tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc
    34201 ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg
    34261 aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt
    34321 atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg
    34381 ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga
    34441 caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg
    34501 ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca
    34561 gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt
    34621 gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc
    34681 aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc
    34741 gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac
    34801 gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt
    34861 tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc
    34921 cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga
    34981 aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga
    35041 caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg
    35101 gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta
    35161 acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag
    35221 gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa
    35281 tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg
    35341 atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca
    35401 cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt
    35461 ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg
    35521 gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc
    35581 tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg
    35641 cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact
    35701 tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg
    35761 atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat
    35821 tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata
    35881 tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct
    35941 tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat
    36001 gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt
    36061 gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc
    36121 ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt
    36181 ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga
    36241 aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca
    36301 ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt
    36361 gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga
    36421 agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc
    36481 aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg
    36541 ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc
    36601 cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa
    36661 agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca
    36721 tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga
    36781 aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag
    36841 aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg
    36901 cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta
    36961 ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag
    37021 ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag
    37081 gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg
    37141 cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca
    37201 gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc
    37261 atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc
    37321 aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag
    37381 gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg
    37441 gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat
    37501 tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa
    37561 gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa
    37621 tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca
    37681 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    37741 gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat cgtgtcacag
    37801 tccctttttt atagtgtttt ttataggtag tattttttag caactgttaa gtcatcattt
    37861 aattcataaa caagtggttg acctgttgga atttcaagat ccatgatgtc ttcgtcagag
    37921 ataccttcga tatgtttagc tagggcgcgt aatgagttac catgtgcagc tactaaaaca
    37981 gttttgttgt ctagtaatgc tggagcaatt tcatcttgcc agaatggcaa agcgcgttct
    38041 aatgtcactt tcaagttttc tccaccagga atatcgcgtt tgtctaacat tgcgtaacga
    38101 cggtcatttg ctgcagaacc ttcgtcagtt tcttccatta atggaggaag agtatcgtaa
    38161 gaacgacgcc agatgtgcac ttgttcgtcg ccgtattttt cagctgtttc ttttttgttc
    38221 aaaccttgta atttaccata gtgacgttcg tttaagcgcc atgatttgat ttgtggtacc
    38281 caaagttgat ctgaatattc taagatcaag ttacatgttt tgattgcacg agttaaaaca
    38341 gaagtgtaag caacatcaaa ttcgattcca gcttctttga ttttgcgtcc gccttctttt
    38401 gcttcttcga ttccttctgg tgctagattt acatcagccc aaccagtaaa ttggttcaat
    38461 gcattccatt cactaagacc gtgacgagaa aaaactaatt ttggcatgtt tagctctcct
    38521 tttaagatag gtttatattt ctgaaatttt cagttctgtt ttattgtaca attttttgga
    38581 agagatttcc atcgaaaaat tagataagat tctatgagaa aacaagtaga gaatataaaa
    38641 aaacgaacag tgcccacgca actgttcgtt tttaggtatt agatgcataa ttgctcgatt
    38701 tctttttcag gtaaattgat atatttataa catgttccaa ctgaaacacc gcattttgaa
    38761 gaaataaatt ggatcgtttc ttttttttca taatagagac gtcggatttt tttgatcgtt
    38821 tttgcatcga tttttggtct tccgccgatt tttccttttt tacgagcttc atctaatcca
    38881 actaatgtac gttctttgat caatgcgcac tccatgtttg ctaggttttc cattaattgg
    38941 aaatagattt ttcccatttc gcgcgtatcg attccttcat caagactaat caagtgcagc
    39001 ccgcgatctt tgaataattg cgtgagttct gttaattgcc gcgtagattt gcctaatcga
    39061 tgaagttggc aaatgactag tgcatcgcct tgttgcatgt cagcgatcac tgactccaaa
    39121 tgaacagcag tagaatcatt ttgttccact tcatatgtct catggaaaat ttcttgacaa
    39181 ccaaagtcag ataattgatc aacctgagtt gttaaatcaa cgtcatttat ggttgtgcgt
    39241 gcataaccaa taattttcat ggtgattcct ttctagcagt gattttctaa ctggatataa
    39301 cagtaaaaat gtagcataaa gtacgtcaaa aagatagttt tttcgcaaat tttccgctgt
    39361 ctttttttat tggatgatca agcagttttt ctttatttac gacggattgc gtcaagaatc
    39421 tcaacgcctt tttctgagcc gacaactact ttagaaacca tgtttaggaa aagcccatgt
    39481 tctactaccc caacaagttc atctaaatag gtagctaagc tttctggctg ttcgatgact
    39541 tctagatgca gatcaatgat gtaatgacca ccatctgtgg ttaagacttc tccgttttcg
    39601 tctgtacgca agacgggttg gaatcttttt tcttcaaaaa tatgaagtag ctgttgggag
    39661 ccataaggga tcacttcaac tgggagtggg aacttcccaa gtttatcgac taatttagaa
    39721 gaatcaacga tccaaatcgt ttcttttgag tacgtagcga ctattttctc gaaaagcaaa
    39781 gcagcgccgc cgcctttgat cccttgaaaa tcagcactga tttcatcagc accgtctatt
    39841 gtcaaatcga ctacggggac ttcatcaata ctctttaacg gtattcctaa tttttccgct
    39901 tgttcttttg tcgcatttga agtagtaact cctgtgatac gtaaaccttc ttcacgcata
    39961 cggcgaccaa tctcttctac catataatag gcggttgaac cggtcccgag acctacgatc
    40021 atcccgtcct ttacgaattt cgcggcttca atgccagcta attgttttaa attcatcttt
    40081 gcgtccacct ttttttagtt tgagcatcgt gctttcctca ctattttagc gtgatttacg
    40141 gtaaaaagat accattcttt tttattccaa taaagctttt tcaaaaaaaa ttgcatctct
    40201 tgttgacatg ttttttcatt cgtgatattc taacaaaggt gctttatcta caggtttctg
    40261 tgaagaaacg tgctgactgt ttatcaaagc atatttgata aggatgcaga cattgcattt
    40321 attttttatc acaaaaaaag aaccacctgg atgtgtggaa ctagacgcaa atcgtgggaa
    40381 ggaggaaaca aggaatgcct acaattaacc aattagtacg taaacctcgt aaatcaaagg
    40441 tggaaaaatc taattcacct gctttgaaca aaggatataa cagctttaag aaaactcaaa
    40501 ctaacgtaaa ctcacctcaa aaacgtgggg tagcaactcg tgtgggtaca atgacaccta
    40561 aaaaaccgaa ctcagcttta cgtaaatatg cacgtgttcg tttgtcaaac ttaattgaag
    40621 ttactgctta tatcccaggg attggtcata acttacaaga acacagtgtt gtattattac
    40681 gcggtggtcg tgtaaaagac ttaccagggg tacgttacca tatcgttcgc ggtgctttag
    40741 atactgctgg tgttaacgac cgtaaacaaa gccgttctaa atacggaact aaaaaaccta
    40801 aagcttaatt ttaattatta aacaaatgaa acttgcttct actatataga agtaattact
    40861 atgaacaata tttcggaagg aggagttacg gatgccacgt aaaggtcctg ttgcaaaacg
    40921 tgatgtttta ccagatccta tttataactc aaaattagta actcgcttga tcaaccgtgt
    40981 aatggttgac ggaaaacgcg ggattgcagc taacattatc tataatgcct ttgacttgat
    41041 caaagaatct acaggtaacg atccattaga agtgtttgaa caagcaatga aaaacgttca
    41101 acctgttctt gaagttaaag cacgtcgtgt tgggggttca aactaccaag taccagttga
    41161 agttcgccct gaacgccgcg ttactctagg tcttcgttgg gtagtaaact acgctcgctt
    41221 gcgcggtgaa cacacaatgg aagaacgctt agcaaaagaa atcatggatg ctgcaaacaa
    41281 cacaggtgct tctgttaaaa aacgtgaaga cactcataaa atggctgatg ccaaccgtgc
    41341 gtttgctcac tatcgctggt aagattctct ctgtttggtc ggcttttata agtcgacctc
    41401 agatagtgtc cttattttta gcaactagat agtaaagaga ggagaaacaa ggagaatgac
    41461 cagagaattt tctttacaaa acactcgtaa tatcggtatc atggcccaca tcgatgctgg
    41521 taaaacgact gctactgaac gtatcttgta ctatactggt aaaatccata aaattggtga
    41581 aactcatgaa ggagcttcac aaatggactg gatggaacaa gaacaagaac gtggtattac
    41641 gatcacttct gctgctacta cagcacaatg gaagggtcac cgaatcaaca tcatcgacac
    41701 accaggacac gtagacttca cagttgaagt tgaacgttca ttgcgtgtac ttgatggtgc
    41761 tgtaactgtt cttgatgcac aatcaggtgt tgaacctcaa actgaaacag tttggcgtca
    41821 agcaacaact tatggtgtac ctcgtatcgt atttgcaaac aaaatggaca agatcggtgc
    41881 tgacttctta tattcagtat caactttaca tgaccgtttg caagcaaacg ctcacccaat
    41941 ccaattacca attggtgcgg aagacgactt cacaggaatt atcgaccttg taacaatgaa
    42001 ggctgaaatg tatacaaacg atcttggaac agagatcgaa gaaacagaaa ttcctgaaga
    42061 ataccgcgaa ttagctgaag aatggcgcga aaaattggtt gaagctgtag ctgaaactga
    42121 tgaagaactt actttgaaat acttagaagg tgaagaaatc actgaagctg agttaaaaga
    42181 aggtatccgc cgcgcaacag ttaacgtaga attctaccca gtactatgtg gttcagcatt
    42241 taagaacaaa ggggttcaat tactattgga cgctgttctt gattacttgc catcaccgtt
    42301 agatatccca gctatcaaag ggattgaccc taaaactgat gaagaagtag aacgtcctgc
    42361 agacgacagc gctcctttct cagctttagc atttaaagtt atgactgacc cattcgtagg
    42421 tcgtttaact ttcttccgtg tgtactctgg tgttttacaa tcaggttcat acgtacaaaa
    42481 cgcaacaaaa ggcaaacgtg aacgtgttgg tcgtatccta cagatgcatg ccaactctcg
    42541 ttcagaaatc tctgaagttt acgctggaga tatcgctgct gctgttggtt tgaaagacac
    42601 aactactggg gacacactat gtgatgaaaa gagtctagtt attcttgaat ccatggaatt
    42661 cccagaacca gttatccaag tcgctattga acctaaatca aaagctgacc aagataaaat
    42721 gggtgtggct ttacaaaaac tatctgaaga agatcctact ttccgtgctg aaacaaacgt
    42781 ggaaacaggc gaaacaatca tcgctggtat gggtgaactt cacttagata tcatcgttga
    42841 ccgtatgcgt cgtgaattca aagttgaagc taacgtaggt gcgcctcaag tatcttaccg
    42901 tgaaactttc cgtgctggca ctcaagccga aggtaagttt gtacgtcagt ctggtggtaa
    42961 aggtcaatac ggtcacgtat ggattgaatt tacaccaaat gaagaaggtg ctggtttcga
    43021 atttgaaaat gcgatcgtcg gtggggttgt tcctcgtgaa tacattccag ctgttgaaac
    43081 tggtttgaaa gatgctatgg aaaatggtgt cttagctggc tatccattag tagatatcaa
    43141 agctaaactt tacgatggtt cataccatga tgtcgattca aatgaaactg ccttccgtgt
    43201 tgctgcttct atggcattac gtgcagcagc taagaaagct aatccagtaa tccttgaacc
    43261 aatcatggct gtagaagttg ttatccctga agattattta ggtgatgtta tgggtcacgt
    43321 aactgctcgt cgtggacgtg ttgaaggtat ggaagctcgc gctggcggcc aacaagttgt
    43381 tcgtgcgatg gttccacttg ctgaaatgtt tggttatgct acaactcttc gttctgctac
    43441 tcaaggtcgt ggtacattta caatgacatt tgaccactac gaagatgtac caaaatcagt
    43501 acaagaagaa atcattaaga aaaacggcgg aaaagctgaa taatttctta aaaccggata
    43561 gtcctgcaaa gagattatcc aaagagacta tatttttaat atagtttcgt gtaaaataat
    43621 tatcgatgat atgggcatgt ggtgtacctt gtgtacatca tatacctatc agacaaataa
    43681 aaaatatatt gtatttttag gaggacattt taaaatggca aaagaaaaat ttgaccgttc
    43741 taaaccacac gttaacattg gtactatcgg acacgttgac catggtaaaa ctacattaac
    43801 agctgcaatc acaactgtac tatctaagaa aaacggcggc caagctatgg cttacgatca
    43861 aatcgatggt gctccagaag aacgcgaacg cggaatcaca atctcaacag ctcacgttga
    43921 gtatgaaaca gatacacgcc actacgctca cgttgactgc ccaggacacg cggattacgt
    43981 taaaaacatg atcactggtg ctgctcaaat ggacggagct atcttggtag tttctgctgc
    44041 tgacggccca atgcctcaaa ctcgtgaaca catcctattg tctcgtcaag ttggtgttcc
    44101 ttacatcgtt gtattcttga acaaagtaga catggttgat gacgaagaat tactagaatt
    44161 agttgaaatg gaagttcgtg acctattaac agaatacgaa ttccctggtg acgatgttcc
    44221 tgtagttgct ggatcagctt tgaaagctct agaaggcgac gcttcatacg aagaaaaaat
    44281 tcttgaatta atggctgcag ttgacgaata catcccaact ccagaacgtg acaacgacaa
    44341 accattcatg atgccagttg aagacgtgtt ctcaattact ggacgtggta ctgttgctac
    44401 aggtcgtgtt gaacgtggac aagttcgcgt tggtgacgaa gttgaagttg ttggtattgc
    44461 tgaagaaact tcaaaaacaa cagttactgg tgttgaaatg ttccgtaaat tgttagacta
    44521 cgctgaagct ggagacaaca ttggtgcttt actacgtggt gttgcacgtg aagacatcca
    44581 acgtggacaa gttttagcta aaccaggtac aatcacacct catacaaaat tctctgcaga
    44641 agtatacgtg ttgacaaaag aagaaggtgg acgtcatact ccattcttca ctaactaccg
    44701 tccacaattc tacttccgta caactgacgt aacaggtgtt gttgaattac cagaaggaac
    44761 tgaaatggtt atgcctggcg acaacgtaac aatggaagtt gaattaatcc acccaatcgc
    44821 tatcgaaaac ggtactaaat tctcaatccg tgaaggcgga cgtacagttg gtgccggtgt
    44881 cgttacagaa atcaaagctt aattcactta agttttaaag taaagcaaca tttattcgga
    44941 cgtaatcgct cggaacgaag gtcttaggat ctttgttccg agctttttta tttattgcag
    45001 gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa agagtcagta
    45061 ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc cttgctttgt
    45121 gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc acagacgtaa
    45181 tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac gctttgccac
    45241 gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata ggcgaaggag
    45301 ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa caccgtattt
    45361 tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct agcgtgtctg
    45421 gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg actcataaat
    45481 acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc gacattgtga
    45541 acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct ggtgtaaaca
    45601 tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg accaaaggaa
    45661 tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa ttaattccag
    45721 taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg gaaacggatg
    45781 gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca aacaaacctg
    45841 cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt gaattcaaaa
    45901 atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt ttccaagcag
    45961 gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg gttatcaaac
    46021 gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt cgtcctgggt
    46081 caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct ggccgtatgg
    46141 gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca gaaagaaatg
    46201 ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact atcaaatcag
    46261 ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg aatgtagcat
    46321 tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa atcttcggaa
    46381 ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg tcattaagac
    46441 aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt aaaccatggc
    46501 gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa tggcgtggag
    46561 gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct aaaaaagttc
    46621 gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac ttggtagcaa
    46681 tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt cttgctaact
    46741 tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt gctgctttat
    46801 cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt gtgctagacg
    46861 ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt gaggaggtgc
    46921 ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa aatcaatgct
    46981 tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca aaactttagt
    47041 aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca tcgtgaacgt
    47101 acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac gtcgcaaagc
    47161 gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg ctgaataagc
    47221 actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa gtacaaacct
    47281 acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac aacatcaaca
    47341 cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa caacggccgc
    47401 atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat cgacttcaaa
    47461 cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc aaaccgttca
    47521 gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt agcacctaaa
    47581 ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa agtcggaaat
    47641 gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga aatgaaacct
    47701 ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact tggtaaagaa
    47761 ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt agctgcatgc
    47821 cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat cggtaaagct
    47881 ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat gaaccctaat
    47941 gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc accagtttct
    48001 ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa atcagacaaa
    48061 cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc acattttgag
    48121 aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga gcatttaatg
    48181 aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac ttggtcacgc
    48241 cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga tggacgtaaa
    48301 catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga atttgcacct
    48361 acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta atttgagggg
    48421 aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa aaacagttcg
    48481 cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa gtgtcgcaga
    48541 tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg aaaaagtatt
    48601 gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa acttggtagt
    48661 aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc gtgcaaaagg
    48721 atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat cagaaaaata
    48781 aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta ggcatcatcc
    48841 gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta cacgaagatt
    48901 taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca acagttgaaa
    48961 tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca ggtatggtta
    49021 ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa ttaactggta
    49081 aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca aaattagtag
    49141 gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct caaaaacaag
    49201 ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta tctggtcgtc
    49261 taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt cctcttcata
    49321 ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac ggaaaactag
    49381 gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac actgagaaag
    49441 gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat tccgcggaaa
    49501 aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg gtttgcaagc
    49561 tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg caatgactcg
    49621 ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat cttatactgc
    49681 caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat gggttgcacc
    49741 agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag tagctcgtga
    49801 agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa aacgtgagga
    49861 aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact gccgaaatgc
    49921 ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa ttagcaacag
    49981 gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca cgcatcaaaa
    50041 ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt atgactgaag
    50101 gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg gataaaacaa
    50161 ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc atgaactatt
    50221 ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac atcgtgagaa
    50281 ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa gtagtcgaag
    50341 aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca aaatctgaaa
    50401 ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat aactcaggcg
    50461 cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact gctaacatcg
    50521 gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc aaaaaaggtg
    50581 acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca gacggttcat
    50641 atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc cctcgtggaa
    50701 cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg aagatcgttt
    50761 cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc gaaacagtta
    50821 tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa aacaaagaag
    50881 gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt gtaaatgtcg
    50941 ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt gagatggaag
    51001 cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct ggccgagttg
    51061 gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt gaagttttag
    51121 ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa gaaaaatatc
    51181 ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt atgcaaacac
    51241 ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca aacgctaaaa
    51301 acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca atgattacaa
    51361 aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt ggtgcaaaag
    51421 ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct gtttcactac
    51481 cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt ggaaactata
    51541 ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta gtagataaag
    51601 tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa tctcgtgagt
    51661 tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac tacgtggcta
    51721 aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa gcttatactc
    51781 gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt tgccgtattt
    51841 gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct agctggtaaa
    51901 aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc caattgcaga
    51961 ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag aagtacctgc
    52021 atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg ttcgtgatgt
    52081 tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat ttggtaaaaa
    52141 cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc gtgcttatgt
    52201 caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca tttcgacttc
    52261 tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg aagttatcgc
    52321 ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc cgtatcggaa
    52381 ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac aacgttacag
    52441 ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa atgaatatcg
    52501 aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa acaattcatg
    52561 gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt ttccaaaaag
    52621 cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa cttgtattga
    52681 acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact gttgaagtac
    52741 cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc gaattagctg
    52801 caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt cgttatgttg
    52861 gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga aaaattgcat
    52921 agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa aatacatttt
    52981 tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata gtagtacaat
    53041 aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac acgtcaaaag
    53101 agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg cttgaacgtt
    53161 tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg tgtaacgcta
    53221 gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga agcagcacaa
    53281 gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt agtagtcttt
    53341 gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc tgctcgcgaa
    53401 aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc gatccaaaac
    53461 atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt gttaaaggtg
    53521 gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga cacgtagggt
    53581 ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt gaagatgcga
    53641 agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa gtaatcggtg
    53701 cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg gttgctgctg
    53761 gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact tctaaatctt
    53821 taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg aaacaattga
    53881 aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta atcggataag
    53941 gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta tcggacgtcc
    54001 tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca gttcagtggt
    54061 taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc tagtggacgt
    54121 tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc aaacctatga
    54181 aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt gttggtcgtg
    54241 gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa aaagctcgtt
    54301 caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc cgtcgtttac
    54361 caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat cttgatgtat
    54421 tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa gctggaatcg
    54481 tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac aagaaattaa
    54541 ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg gctggtggat
    54601 caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa aagacattag
    54661 atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg cacatattac
    54721 tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat ttttcaacat
    54781 gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa tgggggtttc
    54841 accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg tgcctcgatt
    54901 tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag cgactcgata
    54961 tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat tcaatcaata
    55021 cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga tcggtttgat
    55081 tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag aaaaaggtat
    55141 cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc ctgaaagtat
    55201 cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat ggaaatctgc
    55261 tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg taacattttt
    55321 ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg gcgcgcctac
    55381 aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta tctttgctag
    55441 ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat tcggttctga
    55501 aaactggtac aaagtgatga tggagatctt taactataat acagcacctg gtgctacgat
    55561 ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag taaaccctga
    55621 gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac gccctggtaa
    55681 ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg gtgcagtctt
    55741 ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc ttccacaatc
    55801 aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag aaactgcgaa
    55861 acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt aagaaagcgt
    55921 gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa acaatgaacc
    55981 tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa atcattgatg
    56041 cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg caaaacgaaa
    56101 cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt cctgatgaag
    56161 taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa ggcttcttat
    56221 tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg ctaaaagatt
    56281 tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc ttggtcgaac
    56341 gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa attttcaacc
    56401 ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat caaagagaag
    56461 atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat agtgaaccaa
    56521 tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct cgtgaaatcg
    56581 atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc ccgtagccga
    56641 cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata tgtcagtccg
    56701 acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga actgcagttt
    56761 ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg cgtggcaaaa
    56821 gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc aatgtttaaa
    56881 gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat tcgtatgcat
    56941 tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata cgatcttaac
    57001 cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca ggaggtatta
    57061 tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta attcgtcgta
    57121 aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt caaggataat
    57181 tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg ataaacgtgt
    57241 agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa agatcttagc
    57301 tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac aaacagatgc
    57361 tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg aagtgaactt
    57421 gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc gccgtggatt
    57481 gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc cagctcgcgc
    57541 tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca tggcagcaaa
    57601 aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg cacatatcca
    57661 ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg cattagcgtg
    57721 gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt ttgccgctca
    57781 aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga ctgttgaagt
    57841 aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac aagctacagg
    57901 tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg cccgccctcc
    57961 aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta acgtcattga
    58021 acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc gaaaaaccaa
    58081 gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa cctcttgaaa
    58141 gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct tctttgccag
    58201 gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct accatcaaag
    58261 gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg ctgaaaatgt
    58321 atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca gtaactgctg
    58381 gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac atctgtagtg
    58441 tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt ggttatgttc
    58501 aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc gattcaatct
    58561 acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga caccgtgaag
    58621 attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg ttggaagcaa
    58681 tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac ttaactgatg
    58741 aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa aaaatgttgg
    58801 aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg aaacgtgcag
    58861 gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc aaagtccgta
    58921 acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta ggtttaggtt
    58981 tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt tatcgtaaac
    59041 taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact gatttattaa
    59101 tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca actgcagaaa
    59161 aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct gcggctttcg
    59221 tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt gaatcagctt
    59281 tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc ggatacacac
    59341 gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc ttagaacttg
    59401 tctaaaatca cactaataac atcactttat agatgattct tttagagtgt tatgatgttg
    59461 gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg cttggggagt
    59521 aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac agatatgttt
    59581 gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt tttttgatag
    59641 tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat tgaattagaa
    59701 aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga tgtctccttt
    59761 acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg aaaatcgaca
    59821 cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa agtgggaaat
    59881 caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat ggtttttcaa
    59941 aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt tggtcttgaa
    60001 aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact tgagaaagtt
    60061 agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca aaaacaacgt
    60121 gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga tgaagcaacc
    60181 agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa gatcaaagaa
    60241 gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc taatgccaat
    60301 cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa agaaattttt
    60361 tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga aaaactgaag
    60421 agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga agaaaggatg
    60481 gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag ccgaatacac
    60541 catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac agttatactg
    60601 cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg aatgcgcttg
    60661 ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg gatacggata
    60721 ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt ccagaagcac
    60781 agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac tttggtgtca
    60841 gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg ctggatgaaa
    60901 gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga gtagcaatcg
    60961 ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg gcaggacttg
    61021 atccccaggg tagaaaagaa atgatggaga tgttttggcg tcttcataag gaacagcaga
    61081 tcactattgt attagttacg catttaatgg atgatgtagc caattttgca gattatgtct
    61141 atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc tttcaaaaca
    61201 ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt gcagaagaat
    61261 taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa ttagcagacg
    61321 ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt tggtcgttat
    61381 atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt agccagtttt
    61441 tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt gttggcattt
    61501 ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat ccgaggcatt
    61561 cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt tacaagtggc
    61621 ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt gatgaacggc
    61681 ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact cacgcttact
    61741 acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt gaaagccgta
    61801 cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt tgtgcctaca
    61861 ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt tgattttggt
    61921 gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc tttatttgtc
    61981 agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg ctacaaaggt
    62041 ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac gatggttatc
    62101 attgcttttg tattattaac tgtgctactg atctttttga gaggataaac gttcagtggg
    62161 ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcgagc agtgtatgac
    62221 aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt atctgaaaag
    62281 aaagaacgaa aaaattttat aaaaaaatca ttattccaga taacctttta ggaacaaagg
    62341 agagaaaaac atggtacgtt acaaagcaat cattgcatac gacggaacaa attttaatgg
    62401 attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa cattacaaaa
    62461 aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg ccggagtaca
    62521 tgcgattggg caagtaatcc attttgatta tccagaggag cgtccgttag aacgaatgcg
    62581 tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag aaattgtttc
    62641 agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc gagtggatat
    62701 agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt accctcttga
    62761 tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt tcacctcttt
    62821 ttgtgcatca ggaagctcga tagaggataa agtacgtacg atctacgagg ctaagatgga
    62881 ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct tcttgtataa
    62941 gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat tgcctgaaga
    63001 tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta cagcacatcc
    63061 tgaaggactt tatttgtatg aagtgaaata tgaataagag gttgtgacag aagtggtcag
    63121 cttcaagaaa taagaaggaa tttacgaaaa ttacttctca aaattttgca aaatttcggc
    63181 ttattttcga aggagctact tctggaacac cgtgaatcag aggttgtgaa aaagctgccc
    63241 tgcatcaagt aataagaaca accaaacaaa aatagctcgt catatttttc gtttggttgg
    63301 gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaatcagc
    63361 gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
    63421 ttgagctaat accgaaggtc agctgattga acaccgttta agaaagaggt cgtgagcctg
    63481 tcatttaact ctgagtcaca agtaacaatt tcaaaaaaca gcttctcatc tttgatgaat
    63541 atcgtgactt gtggacgagg cgttgataag tgaatcatct ttttgttacc tattttttct
    63601 gtaaccaaaa aacgatggaa cgttgttttg attgccctta atgaggacag tcggaacagg
    63661 tttgtcattt ttatatttaa atggcacaaa tttttctaat tatttgtcat ttagcgctga
    63721 aaatttttgc tatttatcta aaaaagtgat aactacttaa gatgaagcag aatatttacg
    63781 ccatacggca tacaaccgcg aaaaatataa aaaaaagaaa acgattgaac gcgtttttgc
    63841 agtcgcaaag gaaaagcatg gaatgcgcta catcaagtat cgagacttgg aaaacaatca
    63901 ggtgtatacg ctgattgttt ttgctgcaat gaatctgaaa aagttgtcca cttggcagtt
    63961 ggaacatatg tcttcatatc ttttattata caaaaatagc aaaagcacca gtctacactc
    64021 ttagtaaaga ggaaactgat ccttttgtct acattcttaa agtacttaaa ctaagtactt
    64081 tattttactc tttaggattg atacagtagc atctatccct ttattaataa tcttttattt
    64141 ttttaaataa agatgttgtc tccatcttca ggcactaaaa ctttttcgga aattcctttt
    64201 tctttattga aagactttaa ttcttctctt gacagtcccc aatggtttac agcttccata
    64261 tgaacggcaa taattgtgga atctggtatt gctttatgca attcatataa atcgtctttc
    64321 cccataataa gagattctcc aactaaaaaa tgattatctc cagcatttgc tactatgatt
    64381 tctggattat actgatcaat agtttgttta acagcttcat accaaactgt atctcctgta
    64441 aagtatagtg tcttctcagt cgaatgtttg aaaacaatac cgcaaacatt acctacataa
    64501 ttcaagattt ctcctctgcc atgtttgcct tttgttttaa ccaattgtat agattcaaaa
    64561 actgtgtctt ctcgtagtac ctccacattt ttaaaaccat atagtcgaat ttcattcgca
    64621 tcattttcat cttgcacaaa taattttatt tctttaggta ggacctcttt cgctatatcg
    64681 tcccaatgat ctagatgtaa atgtgttaca ataacagcat caataccgtt gataatatct
    64741 tcgactggca ttggcaaatc aactagagga ttcatttgat cttttcttgg tgatggaaaa
    64801 ggacctttcg aaataaatgg tggaagtgtt cctttttccg ctaacattgg atcaattaaa
    64861 aattttttac ctccatagtc taagatagct gttgcattac gaatgtgatg tatattcata
    64921 aacgttgtct cctttttgtt gatatgaaat atttttagta taattcaatt ccatacaata
    64981 aataaaccga acaaaaaaag tcttttacta tttttactta atttatgaaa ggttgtttac
    65041 catgttagac caaacagata taaatatcat tgaggaacta tcaaaaaata gtcgaatcac
    65101 aatgaaagaa cttggagaaa aagtacattt gactggaccc gctgtctcgg cgagagtgac
    65161 aaaactagag gaaagtggag ttattgaaaa ttatacagta aaattaaata tggaaaagct
    65221 agggttttcg attcacgctt ttttaattat aattacgcaa aatttaaatc ataaacccta
    65281 tttatcattt attaaaaatc atgaaaaatt ctt
//
LOCUS       DAZUJO010000005        64675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000005 DAZUJO010000000
VERSION     DAZUJO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.005"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..726)
                     /locus_tag="ACG8OL_000370"
     CDS             complement(61..726)
                     /locus_tag="ACG8OL_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS4816385.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACG8OL_000371"
     CDS             complement(873..1478)
                     /gene="dhaL"
                     /locus_tag="ACG8OL_000371"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS4816386.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACG8OL_000372"
     CDS             complement(1506..2516)
                     /gene="dhaK"
                     /locus_tag="ACG8OL_000372"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS4816387.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACG8OL_000373"
     CDS             complement(2491..2886)
                     /gene="dhaM"
                     /locus_tag="ACG8OL_000373"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS4816388.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            3040..3360
                     /locus_tag="ACG8OL_000374"
     CDS             3040..3360
                     /locus_tag="ACG8OL_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS4816389.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3472..4329)
                     /locus_tag="ACG8OL_000375"
     CDS             complement(3472..4329)
                     /locus_tag="ACG8OL_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4816390.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(4437..5741)
                     /locus_tag="ACG8OL_000376"
     CDS             complement(4437..5741)
                     /locus_tag="ACG8OL_000376"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4816391.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(5738..6247)
                     /locus_tag="ACG8OL_000377"
     CDS             complement(5738..6247)
                     /locus_tag="ACG8OL_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816392.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(6422..8557)
                     /locus_tag="ACG8OL_000378"
     CDS             complement(6422..8557)
                     /locus_tag="ACG8OL_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS4816393.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(8640..10295)
                     /locus_tag="ACG8OL_000379"
     CDS             complement(8640..10295)
                     /locus_tag="ACG8OL_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4816394.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(10297..10611)
                     /locus_tag="ACG8OL_000380"
     CDS             complement(10297..10611)
                     /locus_tag="ACG8OL_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4816395.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            10781..11041
                     /locus_tag="ACG8OL_000381"
     CDS             10781..11041
                     /locus_tag="ACG8OL_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS4816396.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11055..11369
                     /locus_tag="ACG8OL_000382"
     CDS             11055..11369
                     /locus_tag="ACG8OL_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS4816397.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(12099..12653)
                     /locus_tag="ACG8OL_000383"
     CDS             complement(12099..12653)
                     /locus_tag="ACG8OL_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS4816398.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(12779..14708)
                     /locus_tag="ACG8OL_000384"
                     /pseudo
     CDS             complement(12779..14708)
                     /locus_tag="ACG8OL_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(15014..15730)
                     /locus_tag="ACG8OL_000385"
     CDS             complement(15014..15730)
                     /locus_tag="ACG8OL_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4816399.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACG8OL_000386"
     CDS             complement(15731..17554)
                     /gene="glpO"
                     /locus_tag="ACG8OL_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS4816400.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACG8OL_000387"
     CDS             complement(17568..19064)
                     /gene="glpK"
                     /locus_tag="ACG8OL_000387"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS4816401.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(19200..20660)
                     /locus_tag="ACG8OL_000388"
     CDS             complement(19200..20660)
                     /locus_tag="ACG8OL_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816402.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(20650..21945)
                     /locus_tag="ACG8OL_000389"
     CDS             complement(20650..21945)
                     /locus_tag="ACG8OL_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4816403.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(22277..22954)
                     /locus_tag="ACG8OL_000390"
     CDS             complement(22277..22954)
                     /locus_tag="ACG8OL_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS4816404.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22984..24075)
                     /locus_tag="ACG8OL_000391"
     CDS             complement(22984..24075)
                     /locus_tag="ACG8OL_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS4816405.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(24176..24610)
                     /locus_tag="ACG8OL_000392"
     CDS             complement(24176..24610)
                     /locus_tag="ACG8OL_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4816406.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(25194..26651)
                     /locus_tag="ACG8OL_000393"
     CDS             complement(25194..26651)
                     /locus_tag="ACG8OL_000393"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4816407.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(26648..28543)
                     /locus_tag="ACG8OL_000394"
     CDS             complement(26648..28543)
                     /locus_tag="ACG8OL_000394"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4816408.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(28601..30376)
                     /locus_tag="ACG8OL_000395"
     CDS             complement(28601..30376)
                     /locus_tag="ACG8OL_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4816409.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(30426..31922)
                     /locus_tag="ACG8OL_000396"
     CDS             complement(30426..31922)
                     /locus_tag="ACG8OL_000396"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4816410.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(32116..33738)
                     /locus_tag="ACG8OL_000397"
     CDS             complement(32116..33738)
                     /locus_tag="ACG8OL_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4816411.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(34066..35379)
                     /locus_tag="ACG8OL_000398"
     CDS             complement(34066..35379)
                     /locus_tag="ACG8OL_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4816412.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(35624..35848)
                     /locus_tag="ACG8OL_000399"
     CDS             complement(35624..35848)
                     /locus_tag="ACG8OL_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816413.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35868..36290)
                     /locus_tag="ACG8OL_000400"
     CDS             complement(35868..36290)
                     /locus_tag="ACG8OL_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4816414.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(36432..37142)
                     /locus_tag="ACG8OL_000401"
     CDS             complement(36432..37142)
                     /locus_tag="ACG8OL_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS4816415.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(37160..37657)
                     /locus_tag="ACG8OL_000402"
     CDS             complement(37160..37657)
                     /locus_tag="ACG8OL_000402"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4816416.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(37694..38146)
                     /locus_tag="ACG8OL_000403"
     CDS             complement(37694..38146)
                     /locus_tag="ACG8OL_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4816417.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(38281..38886)
                     /locus_tag="ACG8OL_000404"
     CDS             complement(38281..38886)
                     /locus_tag="ACG8OL_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4816418.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(39336..39956)
                     /locus_tag="ACG8OL_000405"
     CDS             complement(39336..39956)
                     /locus_tag="ACG8OL_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4816419.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39958..40827)
                     /locus_tag="ACG8OL_000406"
     CDS             complement(39958..40827)
                     /locus_tag="ACG8OL_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4816420.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40850..41257)
                     /locus_tag="ACG8OL_000407"
     CDS             complement(40850..41257)
                     /locus_tag="ACG8OL_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4816421.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(41276..42565)
                     /locus_tag="ACG8OL_000408"
     CDS             complement(41276..42565)
                     /locus_tag="ACG8OL_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4816422.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(42592..42894)
                     /locus_tag="ACG8OL_000409"
     CDS             complement(42592..42894)
                     /locus_tag="ACG8OL_000409"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4816423.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42919..45012)
                     /locus_tag="ACG8OL_000410"
     CDS             complement(42919..45012)
                     /locus_tag="ACG8OL_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4816424.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(45501..46265)
                     /locus_tag="ACG8OL_000411"
     CDS             complement(45501..46265)
                     /locus_tag="ACG8OL_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS4816425.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(46376..47245)
                     /gene="mdcB"
                     /locus_tag="ACG8OL_000412"
     CDS             complement(46376..47245)
                     /gene="mdcB"
                     /locus_tag="ACG8OL_000412"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS4816426.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(47270..48346)
                     /locus_tag="ACG8OL_000413"
     CDS             complement(47270..48346)
                     /locus_tag="ACG8OL_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4816427.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(48370..48963)
                     /locus_tag="ACG8OL_000414"
     CDS             complement(48370..48963)
                     /locus_tag="ACG8OL_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS4816428.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48967..50643)
                     /gene="mdcD"
                     /locus_tag="ACG8OL_000415"
     CDS             complement(48967..50643)
                     /gene="mdcD"
                     /locus_tag="ACG8OL_000415"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS4816429.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(50633..50932)
                     /locus_tag="ACG8OL_000416"
     CDS             complement(50633..50932)
                     /locus_tag="ACG8OL_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS4816430.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(51034..52680)
                     /gene="mdcA"
                     /locus_tag="ACG8OL_000417"
     CDS             complement(51034..52680)
                     /gene="mdcA"
                     /locus_tag="ACG8OL_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS4816431.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(52687..53598)
                     /locus_tag="ACG8OL_000418"
     CDS             complement(52687..53598)
                     /locus_tag="ACG8OL_000418"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4816432.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            53718..54575
                     /locus_tag="ACG8OL_000419"
     CDS             53718..54575
                     /locus_tag="ACG8OL_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4816433.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(54762..55124)
                     /locus_tag="ACG8OL_000420"
     CDS             complement(54762..55124)
                     /locus_tag="ACG8OL_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS4816434.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(55313..55495)
                     /locus_tag="ACG8OL_000421"
     CDS             complement(55313..55495)
                     /locus_tag="ACG8OL_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816435.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            55470..56909
                     /locus_tag="ACG8OL_000422"
     CDS             55470..56909
                     /locus_tag="ACG8OL_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816436.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(56916..59468)
                     /locus_tag="ACG8OL_000423"
     CDS             complement(56916..59468)
                     /locus_tag="ACG8OL_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS4816437.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(59582..60913)
                     /locus_tag="ACG8OL_000424"
     CDS             complement(59582..60913)
                     /locus_tag="ACG8OL_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4816438.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60879..62357)
                     /locus_tag="ACG8OL_000425"
     CDS             complement(60879..62357)
                     /locus_tag="ACG8OL_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4816439.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(62662..64497)
                     /gene="tdc"
                     /locus_tag="ACG8OL_000426"
     CDS             complement(62662..64497)
                     /gene="tdc"
                     /locus_tag="ACG8OL_000426"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4816440.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        18415 a        13872 c        10972 g        21416 t
ORIGIN      
        1 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
       61 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
      121 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
      181 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
      241 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
      301 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
      361 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
      421 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
      481 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
      541 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
      601 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
      661 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
      721 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
      781 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
      841 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
      901 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
      961 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     1021 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     1081 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     1141 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     1201 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     1261 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     1321 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     1381 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     1441 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     1501 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     1561 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     1621 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     1681 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     1741 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     1801 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     1861 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     1921 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     1981 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     2041 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     2101 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     2161 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     2221 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     2281 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     2341 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     2401 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     2461 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     2521 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     2581 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     2641 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     2701 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     2761 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     2821 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     2881 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     2941 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     3001 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     3061 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     3121 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     3181 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     3241 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     3301 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     3361 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     3421 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     3481 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     3541 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     3601 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     3661 atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa
     3721 atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt
     3781 gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg
     3841 ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc
     3901 ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt
     3961 tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc
     4021 agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat
     4081 ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc
     4141 tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc
     4201 agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc
     4261 tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct
     4321 ttttttcatc acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa
     4381 atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat
     4441 ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact
     4501 gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat
     4561 agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct
     4621 agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa
     4681 gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa
     4741 agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag
     4801 atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc
     4861 cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc
     4921 acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac
     4981 tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca
     5041 tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg
     5101 aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga
     5161 gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata
     5221 tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt
     5281 accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta
     5341 aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt
     5401 ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat
     5461 acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca
     5521 atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca
     5581 tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac
     5641 catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa
     5701 gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt
     5761 ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa
     5821 aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta
     5881 attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt
     5941 atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa
     6001 tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca
     6061 aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa
     6121 gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat
     6181 taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt
     6241 ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat
     6301 ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat
     6361 cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta
     6421 attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca
     6481 tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat
     6541 caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg
     6601 aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg
     6661 ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag
     6721 aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga
     6781 tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt
     6841 aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa
     6901 tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa
     6961 agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta
     7021 cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac
     7081 gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg
     7141 agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt
     7201 cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta
     7261 caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt
     7321 ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat
     7381 tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat
     7441 gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat
     7501 ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta
     7561 gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg
     7621 tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca
     7681 gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa
     7741 tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat
     7801 ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc
     7861 ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt
     7921 ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt
     7981 ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc
     8041 tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg
     8101 tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat
     8161 cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt
     8221 cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata
     8281 aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga
     8341 tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa
     8401 aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg
     8461 tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca
     8521 gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa
     8581 gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat
     8641 catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca
     8701 tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt
     8761 aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc
     8821 cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct
     8881 gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat
     8941 tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact
     9001 ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag
     9061 atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt
     9121 gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt
     9181 aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact
     9241 tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc
     9301 attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg
     9361 gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt
     9421 gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca
     9481 ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt
     9541 ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca
     9601 gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg
     9661 acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct
     9721 gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct
     9781 aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc
     9841 acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt
     9901 ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt
     9961 gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg
    10021 acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct
    10081 aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg
    10141 atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt
    10201 tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt
    10261 ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc
    10321 aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc
    10381 tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt
    10441 gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc
    10501 gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc
    10561 ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac
    10621 ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg
    10681 tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa aataccgata
    10741 aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa
    10801 tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga
    10861 agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga
    10921 tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag
    10981 taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta
    11041 aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa
    11101 cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg
    11161 taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc
    11221 aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt
    11281 ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat
    11341 cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct
    11401 tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt
    11461 tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    11521 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag
    11581 aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct
    11641 catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga
    11701 agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt
    11761 tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa
    11821 ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    11881 ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    11941 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
    12001 cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt
    12061 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
    12121 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
    12181 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
    12241 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
    12301 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
    12361 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
    12421 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
    12481 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
    12541 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
    12601 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
    12661 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
    12721 atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc
    12781 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
    12841 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
    12901 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
    12961 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
    13021 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
    13081 gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg
    13141 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
    13201 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
    13261 ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    13321 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    13381 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    13441 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    13501 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    13561 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    13621 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    13681 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    13741 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    13801 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    13861 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    13921 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    13981 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    14041 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    14101 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    14161 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    14221 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    14281 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    14341 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    14401 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    14461 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    14521 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    14581 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    14641 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    14701 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    14761 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    14821 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    14881 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    14941 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    15001 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    15061 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    15121 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    15181 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    15241 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    15301 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    15361 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    15421 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    15481 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    15541 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    15601 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    15661 catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat
    15721 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    15781 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    15841 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    15901 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    15961 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    16021 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    16081 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    16141 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    16201 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    16261 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    16321 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    16381 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    16441 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    16501 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    16561 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    16621 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    16681 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    16741 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    16801 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    16861 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    16921 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    16981 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    17041 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    17101 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    17161 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    17221 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    17281 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    17341 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    17401 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    17461 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    17521 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    17581 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    17641 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    17701 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    17761 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    17821 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    17881 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    17941 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    18001 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    18061 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    18121 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    18181 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    18241 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    18301 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    18361 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    18421 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    18481 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    18541 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    18601 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    18661 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    18721 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    18781 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    18841 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    18901 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    18961 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    19021 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    19081 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    19141 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    19201 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    19261 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    19321 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    19381 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    19441 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    19501 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    19561 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    19621 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    19681 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    19741 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    19801 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    19861 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    19921 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    19981 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    20041 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    20101 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    20161 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    20221 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    20281 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    20341 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    20401 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    20461 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    20521 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    20581 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    20641 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    20701 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    20761 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    20821 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    20881 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    20941 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    21001 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    21061 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    21121 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    21181 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    21241 acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    21301 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    21361 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    21421 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    21481 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    21541 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    21601 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    21661 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    21721 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    21781 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    21841 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    21901 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    21961 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    22021 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    22081 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    22141 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    22201 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    22261 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    22321 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    22381 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    22441 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    22501 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    22561 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    22621 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    22681 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    22741 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    22801 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    22861 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    22921 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    22981 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    23041 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    23101 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    23161 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    23221 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    23281 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    23341 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    23401 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    23461 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    23521 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    23581 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    23641 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    23701 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    23761 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    23821 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    23881 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    23941 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    24001 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    24061 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    24121 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    24181 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    24241 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    24301 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    24361 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    24421 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    24481 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    24541 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    24601 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    24661 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    24721 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    24781 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    24841 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    24901 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    24961 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    25021 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    25081 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    25141 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    25201 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    25261 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    25321 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    25381 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg
    25441 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    25501 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    25561 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    25621 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    25681 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    25741 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    25801 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    25861 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    25921 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    25981 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    26041 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    26101 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    26161 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    26221 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    26281 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    26341 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    26401 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    26461 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    26521 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    26581 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    26641 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    26701 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    26761 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    26821 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    26881 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    26941 ttggaaaaac tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa
    27001 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    27061 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    27121 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    27181 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    27241 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    27301 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    27361 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    27421 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    27481 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    27541 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    27601 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    27661 ttgcataatc tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac
    27721 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    27781 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    27841 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    27901 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    27961 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    28021 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    28081 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    28141 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    28201 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    28261 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    28321 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    28381 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    28441 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    28501 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc
    28561 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    28621 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    28681 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    28741 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    28801 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    28861 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    28921 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    28981 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    29041 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    29101 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    29161 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    29221 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    29281 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    29341 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    29401 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    29461 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    29521 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    29581 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    29641 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    29701 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    29761 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa
    29821 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    29881 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    29941 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    30001 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    30061 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    30121 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    30181 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    30241 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    30301 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    30361 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    30421 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    30481 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    30541 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    30601 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    30661 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    30721 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    30781 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    30841 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    30901 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    30961 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    31021 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    31081 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    31141 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    31201 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    31261 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    31321 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    31381 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    31441 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    31501 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    31561 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    31621 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    31681 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    31741 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    31801 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    31861 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    31921 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    31981 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    32041 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    32101 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    32161 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    32221 tggaatagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    32281 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    32341 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    32401 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    32461 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    32521 cgtcctagca ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa
    32581 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    32641 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    32701 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    32761 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    32821 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    32881 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    32941 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    33001 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    33061 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    33121 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    33181 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    33241 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    33301 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    33361 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    33421 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    33481 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    33541 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    33601 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    33661 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    33721 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    33781 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    33841 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    33901 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    33961 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    34021 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    34081 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    34141 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    34201 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    34261 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    34321 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    34381 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    34441 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    34501 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    34561 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    34621 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    34681 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    34741 caaacctgtg accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg
    34801 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    34861 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    34921 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    34981 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    35041 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    35101 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    35161 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    35221 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    35281 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    35341 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    35401 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    35461 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    35521 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    35581 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    35641 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    35701 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    35761 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    35821 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    35881 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    35941 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    36001 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    36061 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    36121 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    36181 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    36241 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    36301 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    36361 tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta
    36421 tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg tctaacacta
    36481 gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg tgcgccacgt
    36541 gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc
    36601 agcatcgttc tttgggcttc acgccagttg agacagcgac agatcgctcc ttgttcatca
    36661 tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa aggcaggaat
    36721 gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc gatatcttct
    36781 ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac aagcggattg
    36841 attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat agaagaacgt
    36901 gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat gacttcgttt
    36961 tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc attcaataat
    37021 tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt tacggataag
    37081 atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat tacttttttc
    37141 atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct gtttggggat
    37201 catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta cgataaaaag
    37261 cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt tgcttttctt
    37321 ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt ttaaattttt
    37381 ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca gattgactcg
    37441 ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact aggaaaaaat
    37501 cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct tgtgtcaagt
    37561 aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat gtttcgatac
    37621 taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg tatttattca
    37681 cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc caatcttcgc
    37741 taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct tcgtcagaaa
    37801 gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa ggatagattt
    37861 ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta tcttccaaac
    37921 gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt gtacgatcta
    37981 ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa atacgtacta
    38041 aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg ctaatagaat
    38101 ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca tcccaccttt
    38161 attttgtttg taagactagt tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa
    38221 aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt acgaaaaagc
    38281 tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct tcgttgttcg
    38341 cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg aagtttcatt
    38401 attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata
    38461 ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc gcaactgagt
    38521 agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt cttcaggaga
    38581 caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt tatcttcgat
    38641 tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg gtgttggatg
    38701 tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc ctatttcact
    38761 gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa atacatcttt
    38821 tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag ccaacttctt
    38881 gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag tatagaagac
    38941 tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga atgttaaaaa
    39001 actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg ttcaaaagct
    39061 tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc ttgtctggct
    39121 gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac ggtgttctcc
    39181 taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg tttttttgaa
    39241 attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta aacagcgttc
    39301 aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt gatttctttt
    39361 tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac atcaaattca
    39421 tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt tttgaatgct
    39481 tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc atagtgataa
    39541 tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta ccgacgttgc
    39601 cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc gtatctatct
    39661 gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga cacaatcact
    39721 ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata aactttcgtc
    39781 ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc atagtattcc
    39841 ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag tttattcata
    39901 agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt tttcatctca
    39961 tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct tatgcttgac
    40021 aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact tgttgatttc
    40081 tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct ccgttccaac
    40141 attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt tcacacctgt
    40201 tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca gcaagggaat
    40261 gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa tagctaatgc
    40321 atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc
    40381 attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt ctccttctac
    40441 ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga tattttcttt
    40501 gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga ttgcttcttt
    40561 gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca tttcttcttc
    40621 tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag cggtatcgac
    40681 acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca tagcaggaag
    40741 catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt
    40801 tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt tatttgtttc
    40861 ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc atgtagtaat
    40921 cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta tagaggctca
    40981 tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt tctaaatatt
    41041 ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt tcataaagtt
    41101 tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt gtaatctctg
    41161 attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc agaacttttt
    41221 tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact ttcaattaaa
    41281 atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct actgtatcta
    41341 catcggttgc tgtagcattg ttgaatccca tcgaattaaa gatcgttact agcaaagcgg
    41401 gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct cggcgtccgc
    41461 caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt agcataccag
    41521 gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc gtaccaatcg
    41581 ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa actggacaat
    41641 ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat gccggcacga
    41701 tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact acgaattgga
    41761 tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca gaagcgaatg
    41821 gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc atgacagaca
    41881 aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg atcgactttt
    41941 tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag tacccgatcg
    42001 tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg atcggttgcg
    42061 ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc aagatcaata
    42121 cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc atccataaaa
    42181 ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa ataatattga
    42241 atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa tcattcattg
    42301 cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat ccttcagtaa
    42361 agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca ctcaacactt
    42421 gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat ttcttctgca
    42481 gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt
    42541 cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta tttagataac
    42601 tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg aaacaatatt
    42661 tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact gcgaacgaaa
    42721 attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa tatcacaatg
    42781 gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca tctgtgctgc
    42841 aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt tcatctttgt
    42901 tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt tgaaatcttc
    42961 gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca attcaattag
    43021 ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa aaataatctt
    43081 tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag ctacgctgac
    43141 tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt gtttgccaat
    43201 cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat agcgctcttc
    43261 gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct cccaagtagt
    43321 ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca taggctggat
    43381 ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact tgccatagat
    43441 gattcgatct actcggccac cactttcttt catgacatct attatttgag tgaataactc
    43501 cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt tcaaaaagtc
    43561 ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa gcggcttatt
    43621 tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat ctactaattc
    43681 gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt ctttcagatt
    43741 ctctgctaac agatttccag tagccatccc atggttacag acaacgactg atttataaac
    43801 ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga caagaaaagc
    43861 tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa caaaatgagt
    43921 gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg gattcatgac
    43981 ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga gattttcgaa
    44041 taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa attggatcaa
    44101 ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat ctctagcatt
    44161 gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat tgtccaattg
    44221 aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta tttctgtatt
    44281 tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc agatcaatgt
    44341 aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact tattggaata
    44401 gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga ttttttgagc
    44461 cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat tctgattgat
    44521 aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca gtccttgctt
    44581 tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc gtctcatatc
    44641 ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta gatggatcgg
    44701 gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc gattcaaaaa
    44761 gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct tttccagctt
    44821 caacaaaaaa cctttgttgc gaattgtacg gatctccgga agctttttac tgctcaacca
    44881 gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga actcacttgc
    44941 tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta tttcaattgt
    45001 tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg cttacataat
    45061 aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca aacaaaaaat
    45121 aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat tacaaaaatt
    45181 ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    45241 aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat cgcagatgca
    45301 agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct tgagcgataa
    45361 gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat tgccgatagg
    45421 ctatctctgg cacaccgttt attcagaaaa aggagctgcg acaaggcttt tgtcacaact
    45481 ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc ggcttgccat
    45541 cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg tcgttgtgtc
    45601 ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca gcatctgaaa
    45661 tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac ttcctgaata
    45721 gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag aactttctga
    45781 tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt ttcttcttcc
    45841 caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc tttcttttct
    45901 ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt ttgtctttta
    45961 ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa tgcaagaaat
    46021 cccgtttgct cataactaga aggacctgta cctggaccat aatacccata agaatctctg
    46081 ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa tgaacctctg
    46141 cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc gaatccttgg
    46201 gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata attgattttt
    46261 ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata gccttgctaa
    46321 aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt cttatttagt
    46381 cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct atcttcagca
    46441 tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg ttttcctgac
    46501 aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta gcagcatgag
    46561 caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg catgtgtcat
    46621 ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat gaatcattat
    46681 ctagctcctt agctgcttga attgcaagcc tgatatgcgg ataccctaat atcgcttctt
    46741 catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg gtttttttat
    46801 gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt attttcgata
    46861 aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat agccccattg
    46921 cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt gccttttccg
    46981 cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca tgatgccatg
    47041 ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta agaaataaag
    47101 catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca ggttttggac
    47161 ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc tgagtcactg
    47221 ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat tacttgctga
    47281 aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca aatgcacaac
    47341 ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc agccaactat
    47401 ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac gtacgataag
    47461 cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca atcattttgg
    47521 aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg atcgtattca
    47581 agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa cggacagaaa
    47641 agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc aaatattgat
    47701 agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta gcaacaaacc
    47761 gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt tgatagaaca
    47821 gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca tattttttat
    47881 tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg atctgcagca
    47941 tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc agtaaaaagc
    48001 ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa agataagccg
    48061 tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa aattcccaat
    48121 aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata aacattggtg
    48181 ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt gcgctgtggc
    48241 tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca gctgatactt
    48301 tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct ttctctctct
    48361 ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt tcaatagaat
    48421 ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc gagtcactac
    48481 ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa ttggcaattc
    48541 aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta ctgccggaat
    48601 cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc ctgataatat
    48661 gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct cttgtctata
    48721 cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat gttttgagat
    48781 ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta gtatttctgc
    48841 gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa cgacccattc
    48901 aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat gtgcttgaat
    48961 cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg tttcgacacg
    49021 tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt catttgtatt
    49081 gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat tttgttctga
    49141 agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt ttgtataatt
    49201 attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt cagcaaccga
    49261 acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa tttctttatt
    49321 gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac ctgaaatagc
    49381 atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg cataagcagc
    49441 agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc cgtatgcttg
    49501 gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt tatcttcttc
    49561 gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa cttcaccgtt
    49621 tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga tggcttcttc
    49681 tatgaattta cttgatttac cataatacac agtactaata gaagaagttg gatttttcat
    49741 gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt catttccttc
    49801 tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt cattgtaatc
    49861 gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat aaaatgctga
    49921 gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag cagctttgat
    49981 tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc gttgacgaga
    50041 tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa cgcctgcttc
    50101 ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg cgattaaata
    50161 actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc ctggaatcaa
    50221 accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt ctgaaataga
    50281 taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta caatgactgg
    50341 aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag caacgatttt
    50401 tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag cgattactac
    50461 tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac tttcagggac
    50521 gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa tcaattcacg
    50581 tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa aactattttt
    50641 catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct ggtgtagcac
    50701 cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat ctcgttaata
    50761 cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca gtagattctg
    50821 ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact cctacatgga
    50881 ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc atggatctct
    50941 cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc ttccatctgg
    51001 gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga atttcattgg
    51061 tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg cagctaataa
    51121 tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca cgatgccggc
    51181 tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg taacaccgcc
    51241 aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca aataagcaat
    51301 tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg gtgcattggc
    51361 taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga ctggtttttt
    51421 atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc ctaatggatc
    51481 atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc caggattatg
    51541 tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg tagaagagtt
    51601 tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat attgtcctgc
    51661 agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa aaaagacatc
    51721 tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat ggatgctttc
    51781 aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata cccagttctt
    51841 cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca attcgatagc
    51901 tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg cgtagattcc
    51961 tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag gatctcgtgt
    52021 gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta cccagtcacc
    52081 agggatatct acacgtggta aagaatcgac aacttcgtta acttgaacga tcacgatacc
    52141 atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga aacctgtgta
    52201 caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg gaatcaaatc
    52261 gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt tcattgtgcc
    52321 gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga aatcgatctt
    52381 gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac tagacatgat
    52441 caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg aaagaaaact
    52501 tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa tcagtttttc
    52561 cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt tgctgatttt
    52621 ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg gtttttccat
    52681 aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat gttggtctag
    52741 attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct ttgtcaaagt
    52801 attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca agtccatcat
    52861 ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa tctcttctgc
    52921 gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat cagatagatg
    52981 gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga gacagtgaga
    53041 tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg cttctgccaa
    53101 cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac aattttcatt
    53161 tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct cagcttcgct
    53221 tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt ctttcattaa
    53281 gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc gacaagcaac
    53341 agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa taccgttttt
    53401 ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga taaaaaccgt
    53461 atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt ctaaacgttc
    53521 catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt gtccaggaaa
    53581 taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca ctgctatctt
    53641 agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac aaaaaggtat
    53701 ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa gtattcgaaa
    53761 caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc ttgagccgtg
    53821 cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc ggcagaaaca
    53881 ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca ttagacacat
    53941 tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact tccgtcatat
    54001 tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt atggaaaagc
    54061 atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt accgatcctg
    54121 tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc atgaaagaac
    54181 ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat ctgcaattag
    54241 aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc cgtcatacac
    54301 tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg acagatgatc
    54361 ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc ccaaaagttt
    54421 cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat ttgcccttga
    54481 cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc aaaatgattg
    54541 caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg gacaaaattc
    54601 ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta gaaaataagc
    54661 cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    54721 aaacatttct gtcacaacct cttctctatt gtcctagaca ttcattgcgt tgatttaaaa
    54781 cttccatcaa tcaaatctgc tagttcatct agcttttcta ttttacgtag atcaatagag
    54841 atccaatgtt ctttattcat gtgataggct ggaaaatacc cctcttttcc tcgcaaaatt
    54901 ccgccaagtt caggatcgac cttcaaatca agaatattga tgatcctatg gtcacttgca
    54961 tcatcataga ttttttcttc tggaacagac atcaggaccc caaaccattt tttgcttttt
    55021 tgatgtctga atacgcaata atcaggaaat tttttaaaaa tatattctgg agacaaattg
    55081 tatgtttcat tgatgtgatc gattagttct tttttcgtta gcatctgctt ccatctcttt
    55141 cttattgatt cgctttattc aaagatacca cgtaagaata gaaaaatgaa tacaagtagt
    55201 tgctaatcaa atacttcgat atttttcttt tttatcaact cttaaatgtt caatccgcct
    55261 gtgttggtct tgctaccatc tactattaaa atgtttatta ttgtttgccc acctattttt
    55321 tgattttagt ggtatcattt ttcaagttca tgatgaaatt cttatcatca gtaaatgctc
    55381 cagaaaaata aacaatgatt gaaaaatcag ttgaaacttg accaccgttt attttctccg
    55441 tattcttaga atagaaggga gtctgattga tgtattcact aatgaaaaaa atcattaccg
    55501 aaaaagatat tcgacgtcat gtttctttag tagaacaatt gctgaatcat acaaagataa
    55561 ccgtaaatga attagctgaa atcataggga caaccgaacg aacgattttt tctgaccttc
    55621 aatctattcg ctctcagctg cccgaaggct gggacatcat cagcgatcaa gcaggaatca
    55681 gtttgcaaaa ccagcaaaat ttattaacaa atgacctttg ggagattttt ttcaaacaat
    55741 cagtcagtgt cgaactgctg aaaaatctgc tttttacaaa aaaagttgct gtccctgatt
    55801 ttctagccga ccatgggctt tcttatggga cattgaaaag acatgtgact aaaatcaatc
    55861 agcgtttagc aagttacgac ttgcagatcg atttgacgaa atatacagct tgtattcttg
    55921 gaaaagagcg tgcgattcgt actttttatc atcgattgct gattcctttc actcataaca
    55981 actatttttt cgaggattat tcgatccatg aatcccatta ttttcaattt ttaagaaacc
    56041 tcagtcaaac tgaactagcg gttgaaacag aagagatttt cggaacatgt tggttcttta
    56101 tcaatacgat ccgtataaaa gcgaattgcc gattagattc ttctatccat accaacagca
    56161 cactctcctc cctgtatgac tctgcattga aaaaattgta tttaacggaa ggtatttatc
    56221 ttaaagacac tgagctttct tttgcttctt tctgctttct ggaaagttgg aattacaaca
    56281 ataactatgg acaagaaacc gctcgctgcc tgcatcattc tccattttta gaggtcctcg
    56341 aaacgtttgt agaggaacta gcttcagagc tgtctcttga tcaactaaaa aaaacatctc
    56401 tgactgataa cttggctctc ctcatattaa aatatcatga atcgccgata ttgattgaag
    56461 cattggaccg tcaataccat cgttttttag aaacatatga tcagcaatat gcacacttgt
    56521 atcaacagaa agagaattta ctgaacgatc taaaaaaaga aatagaaatc aatgaaccaa
    56581 attactttct ccaactcttg tctctactga tccaacaaac gattttttct atcaaaccga
    56641 atttgttcaa tgtctatttt attttccaag gagaaccttc atggaaggcg ttcttgcagc
    56701 aggaattaaa agattactta ggaaaacgtg tctgcttctt acctatcgaa ctaaccgatc
    56761 tttccactat ttcttttgaa aaaacagata tcttgatttc caatttccct ctagattctc
    56821 tagatatccc tattgtatac atttcttcta ttccaacgaa aaatgagctc aatcgtttga
    56881 ctgaactcac tttcaaaact tttttatagt aacggctagc gactgtattc tctagctgtt
    56941 ttttcttttt tgcgaaacag tttgttcatg atcccttttt ctatttctac aactaagaat
    57001 agaagaatca catttgctcc aatcaataac tgttgtgtca tcgttaaact tactgtaccg
    57061 atcagctgct ggccgactgg tagaaaaata accatccctt gcaataaagc aaggatacct
    57121 aatgaaagaa aaagcgcttt gttttgcaaa agaccttggt tcaatgacgg cttggacaac
    57181 tctctgcaat tgatcatgta aacagcttga gctaagacga tattttgcag aagaatggtt
    57241 tgctgtaagc tttgcccttc aaactgcata gccaataaat aggcaggaac catgatcagt
    57301 agggagacat agatgatccg aaacaaatga taaccagata ggattccttg ctttggatct
    57361 cttggtgggc gcttcatgat ttctggatct ggcgactcga accctagtgc ataagataaa
    57421 gtgattgtcg ttaccatatt gacccataaa atctgtactg gggacaaagg tagcgggcga
    57481 ttcagcataa gtgcccaaat caagatcaat ccttgagcta atgcagttgg caagaagaaa
    57541 ttgatggttt tttgcaaatt atccaaaatc cgccggccct cttttacggc ttttgcaatc
    57601 gtatggaaat tatcatctcc taaaaccata tcagctgcct gtttgcttac ttcactacct
    57661 ttgattccca tagcaattcc tacatcggat cttttcaaag caggtgcatc atttacgccg
    57721 tctccggtca ttccgacgat ttctccattt ttctgcaaag ctttaacaat tcgcagttta
    57781 tgttctggtg tcgttcgagc aaagacatcg actattggca cttgctgaat caactcttca
    57841 tcagacataa gatccaaatc gatcccttct aaaactttgg cagtatgttt taagccaatt
    57901 tgttccgcaa tcgcttttgc tgtgtctttg tgatcccctg taatcatttt cactgagatc
    57961 cctgcttgtt ggcattcctt tacagctttg actgcacttt cttttggcgg atcgatcaat
    58021 ccggcgatcc ctgcaaaagt taattgggtt aaagtctcat gcgtgagttc ctgtttcgaa
    58081 tctacttttt tataagcaaa tcctaaaaca cgctgacctt ttctcgcaaa ttctgcagct
    58141 gtctgactcc atgcttcttg ttctgcagaa gaaagagttg atttagcaaa taatacttct
    58201 ggtgcacctt tgacgtaaat caccgctcct ttttcctccg catgcatcgt tgccatatat
    58261 ttgtattcag aactgaacgg aatctttgct tctatttttt tcaaaggtag attggctgtt
    58321 tctgtgtgtc gaatcagcgc aacttctgtc ggattgccac ttaaattttc tgttttctgt
    58381 tcttcttttg tttttaattc ctgacagttt ttcataatgg acaacacgca ggcttcttct
    58441 tttgcagcta catccacaac tgtcatttca ttttttgtca atgttcctgt cttatctgaa
    58501 cagatcactg tcatagaacc taatgtttct accgaaggaa gtcctttgat gatcgccttt
    58561 tcttttgcca tttcatgtac tccaacggat agaatcatgg tcaaaacagc tggcaatcct
    58621 tcgggtacca ttgacaccac caaagcaatc gtagccgaaa gaagaatatg ccaatccata
    58681 ccatatcgaa atgtcgtaaa aataactaag aataggctca aacaaagaat gccttgaaaa
    58741 atttgtttgt ttaatcggtt gattttttta atcagaggcg tcgtttgttg ttcgaccgat
    58801 tgcaatgctt gattgatttt accgatttct gtcgtgtttc ctgtttctac aacgacaccg
    58861 actgcagaac cagattgtac aagcgttcct gaaaaaccca tatttttctg gtcgccgatc
    58921 gattcttctt ctgtcaaaac ggcatgactt ttttcagatg cttctgattc tcccgtcaaa
    58981 atcgattctt ccactaataa atcatgtgct tcgattaatc ggatatctgc tggaacgaca
    59041 tctccggcac gtaagacaat gacatctcct tttaccagtt ctttcgcagg gattttttct
    59101 tgcatcccgt ttgatagtac tatcgcttct tgtcccatca gctgcttcaa accattcaat
    59161 gattcttccg cttttctttc ttgccagtaa ctaacgagtc cattgacgat cactacgaat
    59221 aaaataatac ttccttcaat atattcacta gaagcaaatt tcaaaaaaga ggcaatgatc
    59281 aacacgatca tcagcagatc agtaaaatgc tttgctattt ttttccatcg ttttgtctgc
    59341 tgccttgctt ttatttcatt atatccttga tcagtcaggc gtttctgtcg ttcttcttgc
    59401 gacaagcctt ctgttgatgt ttgatagttt ttcaatacat cttctaccgt caatctgtac
    59461 cagttcatcc tatcccctcc tgtttaattg atactgttat tatacgcagg gaagtttttt
    59521 caggcgtatc gattatttca ggggggtctg aaataattcg ttttgcaaaa tgtatctggg
    59581 cctaactgat atttctattg cttattcttt tatatacaaa atttaagaat acaaaccaga
    59641 taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg aaagcaattg
    59701 aagtaaaaaa caccaacaaa gcaagatctt tatatctttg tgtagtgttc cgtttctttt
    59761 ctttaataaa ttgaacatgt gcacacaaga tcgaagacca cacaaataga aaacaactcg
    59821 ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa aaatgtagac
    59881 aaagaccggc aaaaaataac attcctgtaa ttaaaatacc gtgtaaaggt acgcctttct
    59941 tagaaagcgc acaaaaccaa tggggcaaac ttttttcttt tgctgtttga tataaaatcc
    60001 tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa atcattagat
    60061 tgacaattgc tgctccgcct tggcaatgga tcacttgcag catagtcact aaaggacttt
    60121 cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc gttcctatat
    60181 agaaaaagag aatacgccaa ggcagacagt tgattgcttt tttcaaattc ttttgcggat
    60241 cacttgtctc gccaacggtt aagccgatca tttcaattcc agcaaaactg aataaaacca
    60301 taggaaatga gactaccatt ccagcaaagc caaacggtag taattcatcg atctgtccaa
    60361 aattttcttt gattccgata gactgggtat gaaaatctga gacactcaat cctataccca
    60421 ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat tccaattcac
    60481 caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg ctacctccgc
    60541 ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt gctgttaatt
    60601 ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa aatgcccact
    60661 ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta tttgctaaaa
    60721 ataaatcacc catggcccgc atcaaaccat aaatagccaa accgcatatc aaataaacga
    60781 gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat aatcctgttc
    60841 caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc cgtttcattg
    60901 ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc
    60961 caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat gacactgatg
    61021 gctgtgactg caattagatt gatccatgtt ccagctttca tcgtcagtcc gacagaaaat
    61081 ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct ctttttctct
    61141 ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa tctcaaaact
    61201 gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt tacgagcatc
    61261 aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg
    61321 acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt ccaaaaattg
    61381 aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc tggtcctgag
    61441 gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa cagattcaca
    61501 ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc gatattattc
    61561 aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg
    61621 aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac tggattttcc
    61681 aatcgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat gatcggcgca
    61741 aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca tgaaaaactg
    61801 cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc taataagagc
    61861 atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc gatttttgcc
    61921 atatcaaatt tcaaattcaa aaaagtgatg atccaataaa cgactaaaat gacaactaat
    61981 gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa caacggagcg
    62041 agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag ccatgagaca
    62101 acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc acctttttgg
    62161 ggaaacattc cagcaaattc accagagatc aatgagattg gaaaagcgta caataatact
    62221 gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg aatatttcgt
    62281 acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat ttgctttgat
    62341 gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt ttgttttctt
    62401 ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa aacccagtat
    62461 caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa acgaacgatc
    62521 caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg aaaaacaggg
    62581 ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt ctcttgttat
    62641 tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt ccattgcttt
    62701 ggcgattttt tttgtataaa aattgaaaat tcgatcatca ttcaaataag gagtcatgat
    62761 agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg ttagacccat
    62821 tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg tagtatgaga
    62881 agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat cgaacatttt
    62941 ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga atacccaatt
    63001 gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc ctttaacagt
    63061 aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg aagccccaat
    63121 caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc agactgcggc
    63181 ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat ctggagcctt
    63241 tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca tatttttatg
    63301 tttgattacg attcctccag cagcataagg gacatagccc atcttatgag gatctatcgt
    63361 gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt caatcgttac
    63421 gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat acggcacaaa
    63481 ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg catcaacatg
    63541 taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga ttttatctac
    63601 actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta gaattgggat
    63661 tttttgtcca gctaattcac gaattgtttt ttccaaagca ttaatatcca ttcgataatc
    63721 ctcttgaact gggatagcaa ccatctgatc taagccgact ccgcaaatat ctaacgcctt
    63781 catccaagaa tagtgttttg tttgagggac cagccatttc cctaatcttt ggatattttt
    63841 tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa agctttcggt
    63901 catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca tttttttcac
    63961 tttttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcaac gggcgtacca
    64021 taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc cgtctgtcat
    64081 attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag ttgccataga
    64141 agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag ctaataaagc
    64201 aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat gccatgggat
    64261 cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat ggtcgcgggt
    64321 ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat
    64381 ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca ttttataaac
    64441 tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat ctttcatttt
    64501 tcttccctcc atattttgtt ggtcaatgtc ttgcagatac atgtgtttat ctgtaacacc
    64561 tcttcactat agcgccaaaa atgagaattt tttttttata acatcaaatc cacgtagatt
    64621 tttacaaaca aaaataaata aagtcggatt tttctgataa ttcaacgcaa aaaag
//
LOCUS       DAZUJO010000006        63964 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000006 DAZUJO010000000
VERSION     DAZUJO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.006"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            238..1452
                     /locus_tag="ACG8OL_000427"
     CDS             238..1452
                     /locus_tag="ACG8OL_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS4816441.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1483..2910
                     /locus_tag="ACG8OL_000428"
     CDS             1483..2910
                     /locus_tag="ACG8OL_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4816442.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            2933..3859
                     /gene="glsA"
                     /locus_tag="ACG8OL_000429"
     CDS             2933..3859
                     /gene="glsA"
                     /locus_tag="ACG8OL_000429"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS4816443.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            4067..5527
                     /locus_tag="ACG8OL_000430"
     CDS             4067..5527
                     /locus_tag="ACG8OL_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816444.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            5748..7511
                     /locus_tag="ACG8OL_000431"
     CDS             5748..7511
                     /locus_tag="ACG8OL_000431"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS4816445.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            7595..8053
                     /locus_tag="ACG8OL_000432"
     CDS             7595..8053
                     /locus_tag="ACG8OL_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS4816446.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            8162..8845
                     /locus_tag="ACG8OL_000433"
     CDS             8162..8845
                     /locus_tag="ACG8OL_000433"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4816447.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKDGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            8992..9606
                     /locus_tag="ACG8OL_000434"
     CDS             8992..9606
                     /locus_tag="ACG8OL_000434"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS4816448.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            9622..9828
                     /locus_tag="ACG8OL_000435"
     CDS             9622..9828
                     /locus_tag="ACG8OL_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816449.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      10053..10219
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            10367..10924
                     /locus_tag="ACG8OL_000436"
                     /pseudo
     CDS             10367..10924
                     /locus_tag="ACG8OL_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            10982..13021
                     /locus_tag="ACG8OL_000437"
     CDS             10982..13021
                     /locus_tag="ACG8OL_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS4816450.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13130..14281)
                     /locus_tag="ACG8OL_000438"
     CDS             complement(13130..14281)
                     /locus_tag="ACG8OL_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4816451.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(14435..14965)
                     /locus_tag="ACG8OL_000439"
     CDS             complement(14435..14965)
                     /locus_tag="ACG8OL_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS4816452.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(15064..15414)
                     /locus_tag="ACG8OL_000440"
     CDS             complement(15064..15414)
                     /locus_tag="ACG8OL_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS4816453.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(15571..17100)
                     /gene="ahpF"
                     /locus_tag="ACG8OL_000441"
     CDS             complement(15571..17100)
                     /gene="ahpF"
                     /locus_tag="ACG8OL_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS4816454.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(17110..17673)
                     /gene="ahpC"
                     /locus_tag="ACG8OL_000442"
     CDS             complement(17110..17673)
                     /gene="ahpC"
                     /locus_tag="ACG8OL_000442"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS4816455.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(17817..18479)
                     /locus_tag="ACG8OL_000443"
     CDS             complement(17817..18479)
                     /locus_tag="ACG8OL_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4816456.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            18663..20309
                     /locus_tag="ACG8OL_000444"
     CDS             18663..20309
                     /locus_tag="ACG8OL_000444"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4816457.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            20300..21382
                     /gene="uxuA"
                     /locus_tag="ACG8OL_000445"
     CDS             20300..21382
                     /gene="uxuA"
                     /locus_tag="ACG8OL_000445"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4816458.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(21490..23043)
                     /locus_tag="ACG8OL_000446"
     CDS             complement(21490..23043)
                     /locus_tag="ACG8OL_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816459.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            23191..24426
                     /locus_tag="ACG8OL_000447"
     CDS             23191..24426
                     /locus_tag="ACG8OL_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS4816460.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            24399..24479
                     /locus_tag="ACG8OL_000448"
     CDS             24399..24479
                     /locus_tag="ACG8OL_000448"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816461.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(24605..25684)
                     /locus_tag="ACG8OL_000449"
     CDS             complement(24605..25684)
                     /locus_tag="ACG8OL_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4816462.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(25787..26227)
                     /locus_tag="ACG8OL_000450"
     CDS             complement(25787..26227)
                     /locus_tag="ACG8OL_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4816463.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(26284..26694)
                     /locus_tag="ACG8OL_000451"
     CDS             complement(26284..26694)
                     /locus_tag="ACG8OL_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4816464.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(26874..27308)
                     /locus_tag="ACG8OL_000452"
     CDS             complement(26874..27308)
                     /locus_tag="ACG8OL_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4816465.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(27408..28256)
                     /locus_tag="ACG8OL_000453"
     CDS             complement(27408..28256)
                     /locus_tag="ACG8OL_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4816466.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            28645..30411
                     /locus_tag="ACG8OL_000454"
     CDS             28645..30411
                     /locus_tag="ACG8OL_000454"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS4816467.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            30513..31772
                     /locus_tag="ACG8OL_000455"
     CDS             30513..31772
                     /locus_tag="ACG8OL_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4816468.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            31919..33220
                     /locus_tag="ACG8OL_000456"
     CDS             31919..33220
                     /locus_tag="ACG8OL_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4816469.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            33223..34074
                     /locus_tag="ACG8OL_000457"
     CDS             33223..34074
                     /locus_tag="ACG8OL_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4816470.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            34087..34902
                     /locus_tag="ACG8OL_000458"
     CDS             34087..34902
                     /locus_tag="ACG8OL_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS4816471.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            35053..36219
                     /locus_tag="ACG8OL_000459"
     CDS             35053..36219
                     /locus_tag="ACG8OL_000459"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4816472.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            36449..37096
                     /locus_tag="ACG8OL_000460"
     CDS             36449..37096
                     /locus_tag="ACG8OL_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4816473.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            37180..38124
                     /locus_tag="ACG8OL_000461"
     CDS             37180..38124
                     /locus_tag="ACG8OL_000461"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS4816474.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            38259..39710
                     /locus_tag="ACG8OL_000462"
     CDS             38259..39710
                     /locus_tag="ACG8OL_000462"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS4816475.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            39731..40567
                     /locus_tag="ACG8OL_000463"
     CDS             39731..40567
                     /locus_tag="ACG8OL_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4816476.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            40567..41226
                     /locus_tag="ACG8OL_000464"
     CDS             40567..41226
                     /locus_tag="ACG8OL_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4816477.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            41352..42041
                     /locus_tag="ACG8OL_000465"
     CDS             41352..42041
                     /locus_tag="ACG8OL_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816478.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            42047..43003
                     /locus_tag="ACG8OL_000466"
     CDS             42047..43003
                     /locus_tag="ACG8OL_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS4816479.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            43047..43559
                     /locus_tag="ACG8OL_000467"
     CDS             43047..43559
                     /locus_tag="ACG8OL_000467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4816480.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(43815..44363)
                     /locus_tag="ACG8OL_000468"
     CDS             complement(43815..44363)
                     /locus_tag="ACG8OL_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4816481.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(44452..45633)
                     /locus_tag="ACG8OL_000469"
     CDS             complement(44452..45633)
                     /locus_tag="ACG8OL_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS4816482.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGLGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(45869..47377)
                     /locus_tag="ACG8OL_000470"
     CDS             complement(45869..47377)
                     /locus_tag="ACG8OL_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS4816483.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            47536..47727
                     /locus_tag="ACG8OL_000471"
     CDS             47536..47727
                     /locus_tag="ACG8OL_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816484.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            47930..48847
                     /locus_tag="ACG8OL_000472"
     CDS             47930..48847
                     /locus_tag="ACG8OL_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS4816485.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            48840..49913
                     /locus_tag="ACG8OL_000473"
     CDS             48840..49913
                     /locus_tag="ACG8OL_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816486.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            50061..50579
                     /locus_tag="ACG8OL_000474"
     CDS             50061..50579
                     /locus_tag="ACG8OL_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816487.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            50560..50952
                     /locus_tag="ACG8OL_000475"
     CDS             50560..50952
                     /locus_tag="ACG8OL_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS4816488.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            51028..51963
                     /locus_tag="ACG8OL_000476"
     CDS             51028..51963
                     /locus_tag="ACG8OL_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4816489.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            52096..52875
                     /locus_tag="ACG8OL_000477"
     CDS             52096..52875
                     /locus_tag="ACG8OL_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4816490.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            52868..53185
                     /locus_tag="ACG8OL_000478"
     CDS             52868..53185
                     /locus_tag="ACG8OL_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4816491.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            53429..55039
                     /locus_tag="ACG8OL_000479"
     CDS             53429..55039
                     /locus_tag="ACG8OL_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4816492.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            55196..56044
                     /locus_tag="ACG8OL_000480"
     CDS             55196..56044
                     /locus_tag="ACG8OL_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4816493.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            56275..56754
                     /locus_tag="ACG8OL_000481"
     CDS             56275..56754
                     /locus_tag="ACG8OL_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS4816494.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            56787..59015
                     /locus_tag="ACG8OL_000482"
     CDS             56787..59015
                     /locus_tag="ACG8OL_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4816495.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            59144..60787
                     /locus_tag="ACG8OL_000483"
     CDS             59144..60787
                     /locus_tag="ACG8OL_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS4816496.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(60860..61405)
                     /locus_tag="ACG8OL_000484"
     CDS             complement(60860..61405)
                     /locus_tag="ACG8OL_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS4816497.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(61428..61913)
                     /locus_tag="ACG8OL_000485"
     CDS             complement(61428..61913)
                     /locus_tag="ACG8OL_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4816498.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(61903..62715)
                     /locus_tag="ACG8OL_000486"
     CDS             complement(61903..62715)
                     /locus_tag="ACG8OL_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS4816499.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(62854..>63964)
                     /locus_tag="ACG8OL_000487"
     CDS             complement(62854..>63964)
                     /locus_tag="ACG8OL_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS4816500.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        21284 a        11462 c        12487 g        18731 t
ORIGIN      
        1 ttccttatga aaaaatatct ttaaaaataa aaaaagacga aaataaacgg ttttctataa
       61 aaagaagacc gtttattttt tgtctttatt tgtgtgaaaa acgaaaaaga gcacaacaca
      121 tcaagctttt attaattgtc tgtcttttat aaatgcaagt attaattcaa aaaaacagaa
      181 gaaaaaaaca actacacctt cttttatact cgtaatcgta gaaaggaggc gaaaaaaatg
      241 aatacattac ctatcaatac aggagataca gcattcatga ttttatgtac agcaatggtt
      301 tgcctgatga ctcctggtct agcttttttt tacggcggat tagcacggaa gaataatata
      361 ctaacgatca tgtctcaatc ttttctctcc gttgggataa caacgattat gtgggtattt
      421 ggtggattcg gattggcctt tggacgggat atcggcggtg ttattggaaa cccggcagat
      481 ttctttttga tgagacatgt tacaagcttc cccaacatgt ttcatggtgc aacgattcca
      541 tttttaatgt tctttcttta ccaactgatg tttatcgtga ttaccgttcc tttgatgaca
      601 ggagcttttg cagggagatt gaatttaaaa ggctatatta tacttgttat tttttggaat
      661 ctgctgattt atttccctgt ttgccactgg atatggggtg gcggattttt ggatcagatg
      721 ggatttagag atttcgctgg tggagcagtc atccacacga cagctggatt cggctcttta
      781 gcatgtatcc tcacacttgg tcaaagaaag attcctttag gagaaagaag tcagcatagc
      841 agtcttatgg cagctgctat cggtacagga ctgctttggt ttggctggtt cggttttaat
      901 tccggtggtg cacttcgagc agatgaacaa gcagtcaatg catttggttc aacattcatc
      961 gctcttgcat ttgcgatgat tacttggctg atcattgcaa aagtaaaagg aaatggcttt
     1021 gattttgtcg acgtactaac aggatccgtt gctggattgg cagccattac gccttgtgcc
     1081 ggttatgtag aagcaaaaag tgccatgctg attgggatca ttgcgggtat cgtctgtcat
     1141 gctgctgttg attttcgtaa aaagaaacaa tgggatgatg cacttgatgt gtggggtgtt
     1201 catggcatgg gcggattcac aggtacgatt ctgataggga tctttgcatc taacagccat
     1261 ttactaacga atccaagcag ctggtacttc ttagggattc aagtgattgg tgtcatcatt
     1321 actgccgtgt atgcctatgt gctgacaaca gtgattttaa aatcggcgat gcactttaca
     1381 acgatcacta ctacaaaaga agaacaagaa gaaggattgg actttttctt ctttggcaac
     1441 aaactcaatt gaaacaaatt caaatagaaa agaggaagat tcatgcaaca aagcaaaacg
     1501 aaaattcctg caaaaaaatt aacgttcttt ggctttttgg ctatgaccat ctcaatggtc
     1561 gtttcattat atgaatatcc cacattcgcg acctcaggct tcagcttagt atttttcttg
     1621 ttattaggcg gtttgctatg gtttatccct gtggcattgt gcgcagcaga gatggcaacg
     1681 gtgaaaggat gggaaaaagg cggtgtctat acttgggtca gtcgcacatt gggaaaacga
     1741 ttcggttttg ccgcaatctt tttccaatgg tttgagataa cagttggcta cttgaccatg
     1801 ctgtatttct taactggtgc attatcctat gcaacgggta ttggtgctat acaaaacaat
     1861 aaatatctga aattggcaat cctattgatt attttctggg ctattttact ctcacagtta
     1921 aaaggaacca aatacacttc attgattgct cggatcggtt ttgtagctgg tatcttgttg
     1981 ccagcattag tcttgtttgg cttagggatc cattatgtcg cttcaggcgc tccactacaa
     2041 acgatactgt ctatgaaaac attgattcct gatttttcga aattgcctac gctggttgtt
     2101 tttgtttcct ttattttagc atatatgggc gtagaaacat ctgccagtca tgcaaatgaa
     2161 atggaaaacc ctaaaaagga ttatccatta gcaatgtttg tattggttat tctggcaatc
     2221 attttagata cttttggcgg attgaccgtc gctactacag tacctcaaca tggtttgtct
     2281 cttaatacag gggtgattca atcgttagaa ttcctattaa gacacattga tccaaatctt
     2341 gcatggattg cgaaaattct tgcgattctt gtttgtttag gtgtcatcgg agaaattgct
     2401 tcttgggtaa cctctccttc aaaagcactt cacgtagcag cagaagaagg attacttcct
     2461 gaatattttg caaaagaaaa cacacacggt gttcctgctc atttgatgat cgcaaatggg
     2521 atcgttgcaa caatctgggc agccgtcttt acattaagcg gcggggggaa taatctttca
     2581 tttcttgcag cgatgtcatt gactgttgta atctacctga tgttgtactt tatgtttttc
     2641 gcgggctatt tgaagctggt ttttaaagat gacagtcttg aaagagccta tcaggttcca
     2701 ggcggaaaag ttgtaaaagt aatcgttgct tgtttaggtc ttttgacttc actttttgct
     2761 ttttgtatct catttgttcc acctgctagt atcgcagctg gacagcatgg gatttatgaa
     2821 acgattctga ttgtttctta tcttatcgtc ttagttattc catttacgat ctacgcatgc
     2881 cgtcataaat tccagcatgc agaaaaataa aaaatgaaag ctggttattc aaatggaagt
     2941 aaaagaatta gaacaattga ttggaaaaaa tttccactat tatcaaacag gacaagtggc
     3001 ggattacatt ccagcattag ctgaagtgga tcccaaacaa ttaggtatgg cgatttatga
     3061 tttggataca aaacaactga ttgagggagg agacgccgat gtgcgctttg ccatcgaaag
     3121 catgtcaaaa gttcccgtat tgttattagc aatcaaagac aacggaatag aaaaagtctt
     3181 tcaaacaatc aacacggaac caaccggttt tgccttcaat tctatcatga atatggaaat
     3241 caatcatcgc aaacatccaa tgaatccttt tgttaatgca ggagcgattg caacaacctc
     3301 gttgattgcc ggtaaagatg cagatgaaaa atttgagcga atcttgtcat ttatgaaaga
     3361 gatctgtgat gatcctaata tcacattgaa tgaagaaatc tatcattctg aatcaagaac
     3421 aggtgatatc aatcgctctc ttgcttacta tatgaaaggt aatcaaatga tcgaaggaga
     3481 cgtaccggag atactcgatg cttatttcag acaatgctcc ctcaatgtca ccgctacagg
     3541 catagcaaaa ttagctgctg tgctggcaaa taaaggaatt gctccttgga atggcaaaca
     3601 aatcattact gaagaaagtg ctacaatcgt caaatcaatt atgacaactg ctggtttgta
     3661 tgatgaatca ggagagtttt ccgtccatgt tggtgtacct gcaaaaagcg gtgtaggcgg
     3721 cgggttgatg gcagctgtgc caaatcgtta cggcataggt gtcttcagcc cggcactaga
     3781 tccatttggt aacagcgcag ctggtatcca attgttgaaa gatgtagtca aagaactaga
     3841 cgctgacatc tttgaataac cattcttgaa agaggctggg gtaaaagtcc aaacggctca
     3901 aagctaccta caatgaggct gaaattgtga gcttgtttga cttttgtccc agtttctttt
     3961 tttattggtt tttgaaaggg cttttctaaa taaaaaggaa acaaaaatac gatttacaga
     4021 tatgattaaa taaaatcata agaaaaactg gaggcgtgta tcaggaatgc tggaaaatta
     4081 catcgagcga aatatattta gaaaagtgta tctgtgtgaa caattatttg aattccaaga
     4141 aatagacatc gaacaaacag ctatttctct aagggtgact acaccaacga tccttcacga
     4201 tctagaaagt ctagccgagt gtctagaata ttgtataaaa gaacaagtaa gagagaagca
     4261 taagtataaa ctcgtattca aacatggtat tgctctttct gagctcacac agtttctgta
     4321 cggtcaatct tattttttaa agtttttaag ttattatttt aatggtatat tcacgagtac
     4381 agaactggct gatcttgaat ttatttcttt gagcaaagta tatacaatca aaaaaatcgt
     4441 attggatttt tttaaagaaa atgattattt gaaaaataaa gaaatcatta tcccagaatt
     4501 tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag gctatgaaaa
     4561 taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag agactcattt
     4621 ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgaggaa tagagatcgc
     4681 aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa gcgcagaaac
     4741 gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa ttctagactt
     4801 acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt ataccaatga
     4861 gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg ttctcaatga
     4921 cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg acgatgcaga
     4981 aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg cagatggaca
     5041 agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc tgtacggaaa
     5101 aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa gatggaatga
     5161 aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac aagcggggag
     5221 acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt tttatcgaac
     5281 ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt tatttcgttc
     5341 attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt tgtgcgatgc
     5401 tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa tctcattaaa
     5461 aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa aaaaacatat
     5521 ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt ctgtagaagg
     5581 tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt tctctgaaat
     5641 ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat aaagtgtaac
     5701 aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg agtacagtat
     5761 tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct gttgaggaaa
     5821 tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc gatgtaggtg
     5881 ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat tatgtatttg
     5941 attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt cataagcttt
     6001 taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc ggatattcca
     6061 accaacatga aagtaaacat gactatttta cagtggggca cacatctaca tctatcagct
     6121 tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat gtggtagcat
     6181 ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac aatggtggtg
     6241 ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt gatgagaatc
     6301 atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa agcagcaata
     6361 acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac gatctggaaa
     6421 cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta cttcacgtcc
     6481 atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa tatcattggc
     6541 atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga gaaacattta
     6601 atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct cctattgccg
     6661 cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa aaacatccag
     6721 atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct tctgctttgg
     6781 cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa cgggcatatg
     6841 atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta gtcggtggcg
     6901 gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct tacttatcaa
     6961 gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc tctatgctag
     7021 attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca catggtgtag
     7081 aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca gtaaataatg
     7141 gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa gaaacagtaa
     7201 aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa
     7261 atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc acattagaag
     7321 atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt gattcttcaa
     7381 tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct gtagggcagc
     7441 tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg cgtgtcttga
     7501 atattcaata attcattatg ctcaccatcc agataattgt ttatactagt atctggatgg
     7561 ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag aagaatatgt
     7621 caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt tcttttcatt
     7681 tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa agataaagac
     7741 tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac caaaagttgg
     7801 tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa
     7861 tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag aaggagaagg
     7921 ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac atgcttataa
     7981 agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag aatttgaagt
     8041 aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt ctcctgaata
     8101 gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg gaggagatat
     8161 tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa
     8221 cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa aagatggcgg
     8281 agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc gaatcgttga
     8341 tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt ttattactgc
     8401 gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg cactggatgc
     8461 aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa tgattcacga
     8521 acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc aagcgcaaga
     8581 agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt cacgaaagac
     8641 gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata tcccagtaat
     8701 tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat taggtgcttt
     8761 ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa gatatgtcga
     8821 agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg acaaaaaaga
     8881 aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt acttttcatt
     8941 ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa
     9001 cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg ttcagtcatc
     9061 cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc
     9121 ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc gatccagaga
     9181 aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga agctgttatc
     9241 catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat
     9301 gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat catttcagta
     9361 ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca tgtcgagata
     9421 cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa atatcattta
     9481 aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt gaatgctgta
     9541 agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat tgcacgtccc
     9601 gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac agattcaaaa
     9661 gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta cttagaagtt
     9721 ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa ggaaatagaa
     9781 atgccagatc aaacgattag cggattgttt aattacttta acaaataaat atttattccg
     9841 aaaagtgaaa aaacatataa aaaaacacat aaaactaaaa aattatcaaa gaatcctggt
     9901 gcaactgaaa gaatttgcaa cgggattctt tcaacttatt tgttattttt ttctaaaaaa
     9961 tattaaagat tgttaaattt cactatttca tttcaaattc cattgcctaa tcattaggaa
    10021 aagtctatca tgaactcgta aatagaatat agtaataacc ttgttaggtg aggctcctat
    10081 acaaacatag gctgttgccc agaaacgtcg aaagtcgcca atgggtaaaa cagaaactgt
    10141 tggattaagg ctttttccaa tagctaagaa tgttttcttt acgttgtata gtgctaaaac
    10201 tcggacgatg agatgatgct aggtcagaat tcttgttcga aaaggtttct gaccttttct
    10261 ttatattttt gtgactgact tatttgatga gacaaactcg ctcctcacct gacaagtgga
    10321 ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt ggtggaggtc gctcggattc
    10381 acttctggtt gcacaataca accagaaaac acaacaagca aaactgatct ctatcatgag
    10441 agattcatat gtcgatattc caggttacgg aatgaataag ataaatgcag cttactctta
    10501 tgggggagtt gatttattaa atcaaacatt aaaggaaaat ttcaaatttg aagctcccta
    10561 ttatgcaagt atcacatttc aagattttat cgattgcgtt aatgaactgt ttcctggtgg
    10621 agtcaagatc gatgcagaaa aatctctaga tttagatggc gtatatataa acaaaggaga
    10681 gcaagtgatg gatggtaata ctttactcca gtatgctcga tttcgggaag acaaagaagg
    10741 agacttcggg aggatcagaa ggcagcagca agtgatcaaa gctatctctc agcagctgaa
    10801 agacgtaact tcaatattca agctacccaa agcggttggg aaattacttg gtagtatcca
    10861 aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg gatttcctca aagacatatg
    10921 ttagaataaa caggtataaa tagtgaaata aaggaatgac aagcaagaga aggaggaaaa
    10981 aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc gcttatattg ccggcgcagt
    11041 gatcttaata gcagctgcgg gtggcggtta tttttactac cagcactatc aagaaaccca
    11101 agcagtagaa gctggagaaa agacagttga gcaatttgtc caagctttga acaagggaga
    11161 ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca aataaaagtg cattatctga
    11221 aaaagagatc ttagataaat accagaatat atacggtgct gctgatgtca aaggacttca
    11281 gatatcgaat ctaaaagtag ataaaaaaga cgattctact tacagttttt catataaagc
    11341 aaagatgaat acctcattag gtgaattgaa agatctttct tataaaggaa cattagacag
    11401 aaatgatggt caaaccacga tcaactggca acccaacttg gtttttccag aaatggaagg
    11461 aaatgacaaa gtaagcctga ccacacaaga agcagcaaga gggaatatta tagatcgaaa
    11521 tggtgaacca ttagcaacaa caggcaaact aaaacaatta ggagtcgttc caagtaaact
    11581 tggagatgga ggcgaaaaaa cagccaatat caaagcgatt gcttcctcct tcgacttaac
    11641 agaagatgct atcaatcagg cgatttcaca aagctgggta caacccgatt actttgtccc
    11701 attgaaaatc attgatggag caacgccaga acttccagct ggagctacca ttcaagaagt
    11761 agacggcaga tattatcctt tgggtgaagc agctgctcag ctgattggtt acgtgggaga
    11821 tatcacagca gaagatattg ataagaatcc agaattaagc agtaatggaa aaatcggtcg
    11881 ctcaggtttg gagatggctt ttgataagga tcttcgtggg acaacaggtg ggaaattaag
    11941 cattacagat gcagacggtg tagagaaaaa agttctgatc gaacatgaag ttcaaaatgg
    12001 caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa atagcttttg acagtctagg
    12061 aggaaaagct gcatcaactg ttgcgacaac gccaaaaacc ggtgatcttc ttgcgcttgc
    12121 tagctctcca agctatgatc caaacaaaat gacaaacggg atctcacaag aagattacaa
    12181 agcttatgaa gaaaatcctg aacatccatt catcagccga tttgcgacag gttatgctcc
    12241 tggatctacg tttaaaatga ttacagcagc aatcggtctc gacaacggca ctatcgatcc
    12301 aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa gacagttctt ggggatcttt
    12361 taaaacaacg cgtgtcagta gtgatgtatc acaagtagac ttaaaaactg ctttgatcta
    12421 ttccgataat atatatgcgg cacaagaaac gttgaaaatg ggtgagaaaa aatttcgtac
    12481 aggcttagat aaattcattt ttggtgaaga ccttgatttg ccaattagta tgaatccagc
    12541 acaaatttct aatgaagata gctttaactc agatatcttg ctagctgata ctggatatgg
    12601 acagggcgaa cttctaatta ctcctatcca gcaagcagca atgtattctg tttttaccaa
    12661 caatggcaca cttgtctatc ctaaattgat tgcagataaa gagacaaaag ataagaagaa
    12721 tgtcatcagc gaaacagcag tacaaacgat cgtgccagat ctgagagaag ttgtgcaaga
    12781 tgtaaatggt acagcacatt ctctttctgc tttagggatt ccgttggcag cgaaaactgg
    12841 tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa gagaacagtt tcttgtttgc
    12901 tttcaatctt gataaccaag gttatatgat ggtcagcatg ttggaaaata aagaagatga
    12961 tgattcagca accaaacgag catcggaact attgcaatac ctcaaccaaa attatcaata
    13021 aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt atcgtcacag tccttttttc
    13081 aaataaacgg ttttcacgat gtctctttca caacttcttt ttgatttact cacacttcat
    13141 aaactttttt tgtaaaatat ttgagaacaa gtggagaaat aagtgtactt aataaaatca
    13201 cgataactaa aggtgaatag tactgtggtt cgatcaattg actttgctgt ccaatttgta
    13261 aaatgatcaa agccatttct ccacgcgaaa tcataccagc accgaccatc aatgaactat
    13321 gttgagaaaa accggcaatc tgagcaccaa aataacctcc aagcaatttg gtcaaaatcg
    13381 ctacaagcgt caatatagaa ataaagagta gttgttcact aaagtcagaa aaatcaactt
    13441 ctaatcctac actgacaaaa aatactggta taaatactgc atatcctaaa gcctctacat
    13501 tgtggtatac ttcttcttta acctttgtct gactgacagc tatcccagca aaaaaagcac
    13561 caatgacaga gctaagccca acaagatccg ctacgtaaga cattcctaaa caaataacta
    13621 gcgatacgat gataatagaa gaatttgcgt acactttttc tgcaaatgaa agtaggaaag
    13681 gcgcaatcca tttgactaat aagaaaataa aaacaaaata aattagctgt tcaatcaaaa
    13741 caagcggtaa agaagcattg ctagaagctt cccccgttag aaaagatagg ctgaaactta
    13801 gaactaaaac gactaaaatg tcatctacca cagaagcacc caaaattgtt gatccttctt
    13861 ttgtattcac tacgtttagt tcttttaata cttctactga aatactgaca gaagttgccg
    13921 caagaataat cccaaagaaa atcgcttcat ttcccgaaac ttggaatgct ttgcctgtca
    13981 accagccaaa aacaaccggg aagatgatcc caagcaaagc aacgaacata ccaggtcgga
    14041 aatatttttt caaaagagat aaatcacttt ccagaccagc taaaaacatt aataagatca
    14101 caccaatctc cgaaaagtca tggactagaa tatcaggttt gacccaattc aatccagctt
    14161 ggcctagcaa aacaccaaca agtaattgtc caatcactgc tgggatacct aatctccgcg
    14221 agatatgtga gccaatcgtt gttgaaatca atatcaggca taaaatacca ataaattcca
    14281 ttctcatcat tcctttgtat aaaatatagc ccgaacttag cataacatac attattgaaa
    14341 aaataggaaa atatactcac aaaaaaacca acaatccaaa agattttttt ggattgttgg
    14401 tttttatacg gtctatttta ataactaaaa atgattaacg tatagtcatt tttcttattc
    14461 tgatgaaatt gataatctcc tggatgccca tgaaaatcaa aataaagccg aatatttgga
    14521 atagaagcat caaacttttg aatggattga acaataagac gaatccgcca atcaacaaca
    14581 gaatactact ataaacactc caacttttag cttttgtatt catgctgaag aacaactgga
    14641 acaagctgtt gatgataaca gctaatccaa gaagaatcgg caggatagaa acgattgcag
    14701 gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa aagaccactg aacagaccga
    14761 tactccaaga acctgttttc cttttaaaac ggtaagcctc aaaaagattc aacaacccaa
    14821 gcaaaacaag ataggcggtg atcagataac cgataaaatg aaaaacagca gaagggttta
    14881 gcacaatcgc aatcccagaa ataatataaa cagcagctct taataaggcg tatttttgaa
    14941 tattttgttt aatcgtctca aacatataag aaaacctcct ttgacttatt ttaacgtgaa
    15001 ctagtcattt taccaaatta aatgttacaa aatgagcgaa ggagaagaat atctctggaa
    15061 aaatcaactt ctgaggatct tttccatttt tttgcctttt gctagctcat cgatcatttt
    15121 atctaaataa cgaatttctt tcatcaagtt atcctcgatg tcttctactc gtacaccaca
    15181 aacaacacct gtaatcaagg aacgtgaagg attggggaca ggggcttccg caaaaaattg
    15241 ttcgaaagtc acttgttttt gtagttgata ttctagttct gtttgggaat aacccgtcag
    15301 ccaacaaatg atttcatcta cttcctcttt tatacgatct tttctctccg cttttgctac
    15361 atacatagga taaacgcttg caaaacttgt cgtaaaaata cgattttgcg ccataatcag
    15421 tcacctttct ttttttcaaa ttatttacta cttatagtat ataagaaaaa gagaagaaaa
    15481 agcgattatt caaaaaaaca aaaaggattg tgacaaaatt cttgtcacca tccttttttc
    15541 cgctctctga ctttttacaa tcttcatagc tcaagtgcgc atcagataat cgaatgctcc
    15601 taaagctgca gtagctcctg aacccattga aacaatgatt tgtttgtatg gactatctgt
    15661 acaatcacca gctgcgaaaa cgccggggat attcgttgcg ccatttttct cggtcactat
    15721 ttcatttctt ggattcattt cgatcgttcc atctagccaa tccgtattag ggagtaagcc
    15781 gactagaatg aatactcctg aaacagctag tgtatgcatc tccatcgtac ggcgatcgat
    15841 ataggagaga gattctacct gattcgtacc gctgatttct ttcgtcgctg catgtttgat
    15901 aacagtgaca ttcgcaagag agactagttt atcctgtaag atttgatctg cttttaattc
    15961 tggtaagaat tctagaacat atacatgttt tgctaaccca gccaaatcga ttgctgcttc
    16021 aacaccagaa ttaccaccac cgataacagc aatctctttt cctttaaata aagggccgtc
    16081 acagtgtggg caataagcaa ttcctttatt tttaaactct ttttcgcctg gtacattgat
    16141 agaacgccag tgcgcacctg tcgcgatgat ggcggttttt gctttaagtg atataccgtt
    16201 agctaagcca atattaataa aatgttgctc tttatttaag gaaacggcct gttgattcat
    16261 cataatgtcg atagggtaac tacgcacatg actttctact tgctgcatca attttggacc
    16321 ttctgtgtaa gaggtaccaa tcatattttc aatccctagt gtttcaacga cttgtccgcc
    16381 gaaagaatca gcgacaagtc ctgtacggat tcctttacgt gctgcataga tagctgcact
    16441 actagctgct ggtccaccgc caattactaa tacatcataa ggttcttttt caaacaactc
    16501 ttcttcaatc aaagggccag tgattttttc aacaatttgt tcgatcgtca tgcgtccgct
    16561 actaaattct tctccattta aaaacacagt tggcactgcc atgatttttt tatcatcgat
    16621 ttctttttgg aacatcccac cttcaatcat cgtatgagta atatttggat ttaatacagc
    16681 taatatattc aatgcctgta caacgtcggg gcaattatga caagtcaagc taacataact
    16741 gtcaaaatgc aatgtttgtg ttattttttt gattttaaga ctcaaagatt cactgatttt
    16801 cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa gaggaaaatt catgaccgag
    16861 ggggatacca gcaaaaacga ttccactttc tttgccaact ggattgatag aaaaactagg
    16921 tgtgcgcatc gaatctcttt tttcccaaga gattttagga gacatttctg ctatttcctt
    16981 cgtaaattca gctagttttt gactattttc actattatcc aaactaacag aaaaaacaat
    17041 aggagattca atcagtgcta aatattgttc gagattttgt ttagtttctt tgtctaacat
    17101 atccaccacc taaatctttc caacaagatc aaaacttggt ttcaatgttt cgccactttc
    17161 tttccatttt gccggacaaa cttcacctgg gtgggtacgt acatattgag cagcgcgaat
    17221 cttatcaatc agtgcactag cgtcacgacc gattccatct gcattgattt ccatagcttg
    17281 aacgattccg tctggatcaa taataaatgt tcctcgctga gctaatcctt gctcttcatc
    17341 caaaacatca aaaatacgag aaatctgatg agacggatca ccgatcatca cgtattcgat
    17401 tttaccgata gcatctgatg tatcatgcca tgctttgtga gtaaagtggg tatcggtaga
    17461 gcaagaaaag acttcaacac ctaaagattt caatgtactg tattgttctt gtaagtcttc
    17521 taattcagta ggacaaacaa aagtaaaatc agctggatag aagcaaagaa tactccagtt
    17581 ccccataaga tctttttcac tgacttctaa aaattctcct tgatgataag catgtgcatt
    17641 aaattcttcg atttttttcc caatcaatga catagtaaaa cctccaataa taattattta
    17701 tttataatta ttgtaattta gttggagttg caaagtcaat ggatattttg ttatttaata
    17761 aagattactc aaaaacgaag gttaattata aaaatagaag aggtcgcaag aaaatgctac
    17821 tgctggaaat actcgggata acgttgagta acagtttcct gttctttcag catcatgtgt
    17881 aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt tatcaactaa agccttgagt
    17941 aaatcataat gttgttccat gatcgtttcc catggaagcc cctgaatggt cactcgtaac
    18001 cagcggaaac gttctaaatg ggtattatgg tcatctaacc agttccaaac ttcttttctt
    18061 cctgcaattt cgaaacaagc ttcatgaaat aaattatctg cacggaaaaa agcgcgttcg
    18121 tcatttattt tgactgcttt ttcttgttct tctaaaatcg tttctagcac tattcgtgtt
    18181 tgtgtgtcca atttggaaca acattcaatc atgatttgct cttccaactg ttctcggaca
    18241 aagcgagcat tttctgcaga aacaagatta attttggaaa catacgtacc actttgaggt
    18301 atcgtataca cgagttcttg ctgacgtagt tgaatcaatg cttcgcgaat gggagtccgg
    18361 ccgatagcaa gatgttcctc cagtgccttt tcagatattt tccttccagg ttctaagtca
    18421 gcatagataa tttgtttacg aatcgcttga taagcttgtg attgcaagct gcttttcatc
    18481 tttattcctc caaaatcatt tctatgaacg tagtataaca gaagaggtct gaaaaacaat
    18541 ctggtatgtc acattaacta gtaaatttgt ataaatagta aaattttggt tgacatagcg
    18601 atttttattg tataaaattc acttgaatac aaaaactaat atgttagttt aggaggggaa
    18661 aaatgattga tttaaacaac tggaggaact ttatccctga attatctcaa aaagaagtag
    18721 aaatacctca ttttgaccgt gcatcattaa gagaaaatgg acaaaaaaat cctgtctgga
    18781 tccatttcgg tggcggaaat ctttatcgag gatttcatgc agaaatcgct caacgtttag
    18841 cggatctagg cgaattgtcc actggtgtca ttgtttgtga aacattcgat gaagaaataa
    18901 ttgaaagggc ttaccaagat tttgatgatt tgattcttga agtggtcatg ccaagcagtg
    18961 gagagttcgt caaacgtata ttagctgcga cagctgacag tctgtattgt catccagatt
    19021 gttctgaaca ctttgataag atgattcgac actttgaaaa tcctagttta caattagtta
    19081 cttttgctat tactgaaaaa ggttatcgat tgaaagatac aaataataaa tatataggac
    19141 cgattgcagt cgatataaaa aatggtccaa agaagccatc acatacaatg agtattcttg
    19201 ctgcgctact ttataaacgt tttttatctg gagcttggcc gattgcactt gtctctacag
    19261 ataatttttc acaaaacggt agtcaattac gtacttcttt gctaacgatc gcaaaagaat
    19321 ggcaaaagga aggttatgta caaaatacat ttatccaata cttatcgaat gaagaaattg
    19381 ttgcgtttcc atggacgatg attgatcgta tcaccccgaa tccttccttg tcagttagca
    19441 aaatgttggc aaaagagggc ttttcaaata tgtcgatttt ccaaaccgca aaaggaaata
    19501 atattgctcc atttgtcaat acagaggaca tccattattt aatcatggaa gatcattttc
    19561 ccaatggacg tccgaaattc gaaaaagctg gggtattctt gactgatcgt gcaacggtag
    19621 ataaagcaga tacgatgaag gtgacgactt gccttaatcc tttgcatacg tctttagcta
    19681 ttatgggttg tttactgggg tataggagca tagctgagga gatgaaagat ccagatttat
    19741 cgctgctagt ttctgaaatc gggtacaatg aaggcctccc agttgtggag tctccaggta
    19801 tcattgatcc aaagatcttt ctggaagaag taattactgt gcgcttttct aatccaaata
    19861 ttcctgatat gcctcagcga atcgccacgg atacctctca aaaggtggcg attcgttatg
    19921 gtgagacgat taaaaaatat atggaaatag aagttaaaga cccgcagaca cttgtattca
    19981 taccattgac tctcgctgct tggcttcgat accttattgg cattgatgat caaggaaaac
    20041 cattcatccc gagtccagac ccactattga cagactttca gcataagctt tccgatattc
    20101 aactgggtac gcaagataca tcacatattc atgagaaaat ggctcctatt ttgtcaaata
    20161 aagaaatttt tggtgtagat ctttaccaag cagagcttgg cagaaaaata gaagaaatgt
    20221 tcatcaagat ggtagcagga ccgaatgccg tacgaaaaac attacaggat tacttacaag
    20281 aacatggagg aaatttatca tggaaatgac ttttagatgg tacggatcac aggaagaaaa
    20341 gattacctta gatgaaatca gacagattcc tgcggtcaaa gggattgtag gcacattgat
    20401 ggatatacca gtaggagagg tatggcccag ggagaggatc aaagaactaa aagaagaaat
    20461 tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc aatgtgcatg atgccattaa
    20521 aattggatta ccttcccgtg agcagtatat cgaaaactat aagcagacaa tccgaaactt
    20581 ggcagaatat ggaatagaag tcatctgcta taattttatg cctgtatttg actgggtgaa
    20641 atctgacctt gactaccgct tagaagacgg ctcatccacg ttagctttta tatctgatga
    20701 tattccagat gatccaagag aaatcgtcgc gcgcatcgaa cattccagca atgaatttga
    20761 attgccggga tgggaacctg agcgcttagc tcatatcaaa tccttgcttg aagcctatgc
    20821 atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat tttctccaat caatcatccc
    20881 aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca gatgatccgc catactcgat
    20941 ttttggatta cctcgaatcg taaagaatcg agaagattta gattggttgt gccgcatagt
    21001 agacagccct tataacggaa tcactctttg tacagggagt attgcagaag atccagaaaa
    21061 tgatgtatat gggattttag cagaattttg tcaaagagat cgaatcccat ttgcacatgt
    21121 tcgaaatatt aaatttttag ctaatcgaga tttctatgag gcaccgcata aatccgaata
    21181 cggttcatta gacatgtacc gcatactaaa aacaatgtat gacaacggat ttaacgaata
    21241 catccgtcct gatcatggaa gaatgatctg gaatgagaca ggaagaccag gatacggctt
    21301 atatgatcga gcattaggtg ctgtttatct aaatggatta tgggaagcaa tcgaaaaagg
    21361 aaacaaaagt aaagataatt agtgaaaaaa actgtaattt tattatgtaa ataagaagga
    21421 tgttcaaatt cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc
    21481 ttatttggtc taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc
    21541 ttttaattca aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt
    21601 gatgattcct tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca
    21661 gtcgctgata acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt
    21721 tgaaaaaatt tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata
    21781 gttcacacca atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc
    21841 ttcaaccatc cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg
    21901 ttctctgaaa tccatataaa attgttcaat ggcggtttgg acttctttca acacgctatg
    21961 ttcttgataa agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc
    22021 attataaagt ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat
    22081 aaacaaatca aatacagtac aaaaggactc caccatttct cttgaataaa gaacatgagg
    22141 aacgtttgct ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt
    22201 cgtgatagaa agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag
    22261 attaggtcgc aaaatatcta cagagggatc aataaaaaag tcaatatatt catactctgt
    22321 gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa
    22381 tagaaaatat aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa
    22441 aaaagtattc atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt
    22501 acttgaaaag atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc
    22561 agaaaaagac tttctgccat tattagaaaa ggagatagac aaaccgtaca atgctaaaaa
    22621 tgcattaatc tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact
    22681 taaactgata atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac
    22741 tgattcgttg cagtatcttc caaatagacg ataatagcag taagccggat caattccttc
    22801 aaaattcaac tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg
    22861 ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa
    22921 atgatctgct aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat
    22981 atctaatttt gtattgcttt ttttcttcaa gagaaacgtt ttaatcgctt cttgagtttg
    23041 catgaaagag gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc
    23101 ataaatttag acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg
    23161 catacaaaaa aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca
    23221 tttgcagcct taagcttatc gcttactata aatcagacag tccacacaga ggagatagaa
    23281 acagccaaat ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa
    23341 ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa
    23401 gcaaaaattg actattcagg agacttagca cctatactac aatcagctgt tacatttgag
    23461 aatgttgatc ccgctttgtc gattggctat gacgagaaca ctttgactat ccaacacaag
    23521 caagcgatat ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt
    23581 actgtcaagg tatcagatgg actaccgaac gatcatcatc atttaacgcc gtatagtcag
    23641 cggcagacaa ttgagcaaga ttcagctatc gactcaggag gagatcttgt tgaagaacca
    23701 acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga
    23761 gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca accagagcca
    23821 gacaatgaat caaaacctga aataacccct ggagaaaaac cagatgtaga cccagaagaa
    23881 aaaccagatg tgactccaga accagatacg gattctggaa accaaacagt accggaaaca
    23941 aatccagaca cagataatga aactgaaaat ccagaaaaac cagaggtagc cccagaagaa
    24001 aaaccagatg taactccaga accagatacg gattctggaa accaaacagt accggaaaca
    24061 aatccagaca cagataatga aactgaaaat ccagaaaaac cagaggtaga tccagaagaa
    24121 aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa
    24181 ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca
    24241 attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa
    24301 tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa acgaaaaata
    24361 aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc ggaatcattc
    24421 tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga tctctctaac
    24481 atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag aaaatgaccc
    24541 caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt tcttcatatt
    24601 tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc actttcaata
    24661 catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc
    24721 gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca
    24781 tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc gtaatcagga
    24841 tataaacaac atctcggatg cctccttggg aataagctat gaaagataga ggaatacaag
    24901 aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc gctaaactca
    24961 gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta tttaaaagag
    25021 cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta tcggcaaaat
    25081 agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt gagaatacaa
    25141 ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata gcaaacgcca
    25201 ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct
    25261 gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca ggatctgcat
    25321 cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat gtttgatgaa
    25381 taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc agtgtataaa
    25441 tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt tgaacaagat
    25501 gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata cttctaacta
    25561 agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt tcattcgcca
    25621 aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata aacacgttat
    25681 tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt
    25741 tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag ccctttttct
    25801 ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta tccaattctt
    25861 cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct tgtttttgca
    25921 acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc gtttcacgct
    25981 gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc gtaccagaat
    26041 ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc cataaaacta
    26101 acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga gtgtagtgtc
    26161 taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact aaatatagat
    26221 cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt tacacaaaaa
    26281 agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag cttttgaata
    26341 cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag aaagaccttc
    26401 gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg aataaaggga
    26461 aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga tcaattctaa
    26521 tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt
    26581 ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg gattatgcac
    26641 ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt tcataaaatt
    26701 accaccttta attatatttt atacaattga attttacaca atataattgt ggaaagcaaa
    26761 gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg ctttctcgaa
    26821 tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa acattacata
    26881 tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac ctgatgaagc
    26941 atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa gtccatcatt
    27001 tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg tgtgtcctcg
    27061 atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt aggagcagtc
    27121 aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa ggtaaaaatg
    27181 acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc atcaaagatt
    27241 gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat ttccgttcct
    27301 ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct tttattatac
    27361 cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca ttcgattgga
    27421 tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac aaaggcactt
    27481 ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt cacaacgatc
    27541 aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag ggtaaggcca
    27601 cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc actcatgacc
    27661 ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat tgaagtgatt
    27721 gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag attaagagaa
    27781 acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa atatttttta
    27841 aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata taaaatcgca
    27901 actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc tgaagtaatc
    27961 attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa aagtacttta
    28021 aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat attcaacact
    28081 aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc tagaccgctg
    28141 acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac tcctaatatg
    28201 aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc tttcatttat
    28261 tcacttcctt aattcattaa taatactctt atcttattat aacttacttt tcaccttaag
    28321 ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca ttcttctaga
    28381 atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg gagaaattga
    28441 tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa cttttaaaag
    28501 ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa aaaactgtta
    28561 ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt gatgacgtca
    28621 tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg accggaaagt
    28681 gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac taggaaaaat
    28741 gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac tgaaaaatgg
    28801 tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga ttattgggaa
    28861 attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt tggggaagat
    28921 aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa tgtgattact
    28981 gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt cactattcca
    29041 gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc aaacggggac
    29101 aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga ccgagaagac
    29161 tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac ggaccttggc
    29221 atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa atacgatacg
    29281 attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa aaacttgatt
    29341 gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa tcatatgggt
    29401 gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat ctataaagac
    29461 tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt accagaaact
    29521 gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa actaaataca
    29581 gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat aaaagaattc
    29641 gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa
    29701 ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga gatttggcat
    29761 tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta tgcatttaca
    29821 gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga
    29881 atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt caacttatta
    29941 gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga attgatgaag
    30001 tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta cgggacagaa
    30061 atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg ggagaaggag
    30121 gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag gaaacagttg
    30181 tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg tgaagataag
    30241 ctctatttca cacggaaact agagggacag atcatctatg tttactttaa tcagtcaaaa
    30301 gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg tcagttgtta
    30361 gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg aaaactgtta
    30421 ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa taagggtgta
    30481 acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg
    30541 gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc aatggtaact
    30601 cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt gattctggat
    30661 ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca
    30721 agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat gggcctgctg
    30781 gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta ggacagcaag
    30841 gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca gacaaagccc
    30901 aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc cttgttttgt
    30961 actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg gagaatctat
    31021 ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga tttttagcta
    31081 aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc tatgtatttg
    31141 gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa
    31201 tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa
    31261 gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca atcattgacg
    31321 gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta gccaaaattc
    31381 ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga tgggtagtca
    31441 gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta acgaatcagg
    31501 aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg
    31561 aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa tatgaaacag
    31621 ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga
    31681 tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa
    31741 caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc atttttataa
    31801 aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat tatcttgctc
    31861 ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg gcattcagat
    31921 gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt cacttattcc
    31981 agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt tcgccatatt
    32041 cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag gattggttac
    32101 tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag gcactttgca
    32161 attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag acgcttatcg
    32221 agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg aagtcataca
    32281 taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc ttctcatgat
    32341 ttttacgatc attttcccag tcctagttac attgttcact gcatttacta attatgactt
    32401 caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact tttttagtat
    32461 cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg
    32521 gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca
    32581 ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac catgggcggt
    32641 cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg taggtgccat
    32701 caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca gtgcaatctc
    32761 ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg gctggttagg
    32821 atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag aagacttata
    32881 tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc
    32941 tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga atttcaataa
    33001 tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg
    33061 ttctactgat attcttattt cctggatcta taaacttacg actggaactt ctccgcagta
    33121 ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg tgtcattgct
    33181 ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa atttcattcg
    33241 agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct tttaatactg
    33301 tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa gtcaggaaat
    33361 attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt tactcggcta
    33421 ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc tgtcctaaca
    33481 atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg ctatcgtttt
    33541 atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc gactatggca
    33601 gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa gtactggttc
    33661 ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat gaaaggatac
    33721 tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc agggcatttc
    33781 aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt tcaagcatta
    33841 tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct tcgaacacct
    33901 gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac aaaccaaaga
    33961 gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct attattcttc
    34021 tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg ataggagcag
    34081 agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc aactcaagtg
    34141 tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt atttttagtt
    34201 tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc gattccatta
    34261 gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa attttctgaa
    34321 cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt gacccctgaa
    34381 attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac ttttattgat
    34441 tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg ccgctatacg
    34501 atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga tttccaacga
    34561 tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc ttatagttta
    34621 agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc ttcctttttc
    34681 ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga gagtgcaaat
    34741 ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg gtttatccac
    34801 tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct gcttttatgg
    34861 gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta agcgatttat
    34921 gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa
    34981 ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt agttttttta
    35041 aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg ttctttctcg
    35101 ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga tacggacact
    35161 cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat tacaagtaac
    35221 gcttctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga gggaagtatc
    35281 caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg aaaagattat
    35341 ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga agatatccat
    35401 gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac tttgacttat
    35461 tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat caataaagtc
    35521 catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt
    35581 ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc aactgggatc
    35641 acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga acatttagaa
    35701 gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac gtttgatcat
    35761 atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa
    35821 gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat cagccgacaa
    35881 caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg ttatgtccct
    35941 ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat tgatttggaa
    36001 agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt tcatcaagtc
    36061 caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga tctatattta
    36121 caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata tttgcttgtt
    36181 caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa gtactgttta
    36241 aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt ttttgtctaa
    36301 cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata ataaaagttt
    36361 ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg taatagaata
    36421 ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata ctagtttatt
    36481 tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat ctgggaatac
    36541 gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa aagcaagcac
    36601 atcttcgtct agccagacaa aaagttctag tgaagcaagt actcagtcag atatcaaagt
    36661 gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg caattactag
    36721 tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg tcgatgatca
    36781 aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag agaatccaga
    36841 aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc tggatgttga
    36901 taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac
    36961 ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag tcaaagtaaa
    37021 agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag tactgtcaac
    37081 tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt ttagtcatga
    37141 tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga aacgaccttt
    37201 gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg catgactacc
    37261 caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa gagctgtacg
    37321 atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt agggagtatc
    37381 aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc tcttgcgtat
    37441 gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg gatgagtgtc
    37501 caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa
    37561 ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga ttttgaggcc
    37621 acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg aatcaaattg
    37681 cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa tcagtttcca
    37741 ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga tacagaacaa
    37801 gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga gtatatcaag
    37861 ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc
    37921 attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct atgtggcgca
    37981 agcatgttac agattggtac cgagcttcat aaagaagggc cggaaatttt ttctagaatc
    38041 ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga cgaatttaaa
    38101 ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc ttcgtctttt
    38161 tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta tgttagacga
    38221 acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca acagaattag
    38281 caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat atacagaaaa
    38341 aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta
    38401 attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt atcccatttc
    38461 ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga tctcgtttat
    38521 ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa gcgataggtc
    38581 tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt acagacccag
    38641 tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc ggatcaagtg
    38701 gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct agtgatggcg
    38761 gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag
    38821 ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct attgatttcg
    38881 gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga agtatccatt
    38941 ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga aaaaaacggt
    39001 tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa gaggcagtac
    39061 ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg acagagattc
    39121 cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat ggcgcagaaa
    39181 cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga aaagaagaac
    39241 ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc gctacttaca
    39301 tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta catcaagaat
    39361 acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc gaagatttgc
    39421 aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa gtagaaggtt
    39481 tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat acacagttgg
    39541 ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa gaaaacatgc
    39601 ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt caaatcggct
    39661 atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag cagaaacaag
    39721 gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct tttttatttt
    39781 aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta atgtagaaaa
    39841 aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt attataatga
    39901 tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta aacggttaaa
    39961 tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg tttcaaaaag
    40021 agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa aaaaattctc
    40081 cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa aagatgattc
    40141 aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac ccaatactag
    40201 ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca atgccacgaa
    40261 tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa atgattatta
    40321 tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac tggagaacgt
    40381 ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa
    40441 gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga aaatatctga
    40501 aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg agtcggtgag
    40561 tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact tcctttcgta
    40621 ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt aggaaatctg
    40681 gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa tgtatttatt
    40741 cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt tctgttatat
    40801 tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt tacgattacc
    40861 agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc ttcaggtcag
    40921 tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata tatcgttttg
    40981 ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga tctagtgaaa
    41041 ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc aaagatcata
    41101 ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat ttattgggtc
    41161 atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat gcatcagtca
    41221 atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt tgtttcttgt
    41281 atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg
    41341 aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta taccaatatt
    41401 gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac caaggaattg
    41461 tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga tgctatgagc
    41521 tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt ggacattata
    41581 attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc cctacaggaa
    41641 taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc tctggtacat
    41701 ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa gctttgacaa
    41761 accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg
    41821 taaaatattt tccaacagat caattgtaca aagaattgaa aagacgttca gaaaatcaag
    41881 atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct agtcaggcaa
    41941 tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac
    42001 aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt actatcctta
    42061 tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc gaggattgct
    42121 ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt
    42181 gatcgaatta tttcaaaaaa agcaccatcc aatcgctgtg atccaaaatc cccaagtagg
    42241 acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa atagctctgt
    42301 cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt cacctccagc
    42361 gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga aacagttggc
    42421 aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct ggctgcccga
    42481 aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa gttatgcaga
    42541 taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg gatatattgg
    42601 attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc ctagccgagc
    42661 gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga aacattttgt
    42721 ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag caggagataa
    42781 aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag gaatgattga
    42841 attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga aaaagtggtc
    42901 acttatgcat catttggaac ttattagtca cctgctagat catccaaaag attggcttaa
    42961 atattattca aaagttcagg aactatatga agtgatccaa tgattagata atgtaaatga
    43021 aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa gaagaagtag
    43081 aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa ggctctcctc
    43141 aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag
    43201 aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta gtctcaagcg
    43261 tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt ccttatttat
    43321 cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca aaaccgttct
    43381 tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt atcgatactt
    43441 atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact tatcaaggga
    43501 tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc atcaattgac
    43561 tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac aatcatatga
    43621 tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa
    43681 tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct ttctgaaact
    43741 cccttcatcg agaggtcgtg acatagcttt tgtcacgacc tctcggctta tcctacgagt
    43801 attattcgat acatttatat caataatcct ttttttattt tatctgcagc ttcttggaac
    43861 gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt caactgtgct
    43921 aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc tccacgtttt
    43981 ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg gactaccgtc
    44041 ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta acaatttcct
    44101 actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg agcatattgg
    44161 tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc agaaacaaaa
    44221 taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta agtaatgatc
    44281 ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc agaaggaccg
    44341 ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca agtgaaaaag
    44401 ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat attataccag
    44461 caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca aaattttcaa
    44521 tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg caccagaaat
    44581 gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa ttgctgttgt
    44641 acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa attcatttgt
    44701 aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg ggattccgct
    44761 gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga tgccgaatat
    44821 cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa gcatagcagc
    44881 aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt ggaaaaatgt
    44941 ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata catagggatt
    45001 tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag tgatcacata
    45061 tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg tagacatagc
    45121 cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct tttttatgga
    45181 aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag attccaattt
    45241 gcccaacccg ttgattttac ttaatcctag accaacagct ttcataaata atggaagaat
    45301 cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca tcaatacgga
    45361 aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt caacaccttg
    45421 cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa ccgctgtacc
    45481 aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca tcacaattag
    45541 tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac
    45601 aaataaacca ataataccta ataaataact caaataatca ctcctattcc aaattccgtt
    45661 ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca ttactttttt
    45721 agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa aggtttaaaa
    45781 agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc taatagtttc
    45841 gttttcaaca tttataagta aactttttct attggtaata tagattttcg gaagggtaga
    45901 caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca tttttattga
    45961 ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca taagcaactt
    46021 gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca ttgatgctta
    46081 gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt tcaagaacta
    46141 atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact gcatttaaga
    46201 taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg acttcatctg
    46261 gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct tgaatcttat
    46321 ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa ccgacttctt
    46381 ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc
    46441 attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg acaacggtat
    46501 catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat tgataaacag
    46561 attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc tcaacatccc
    46621 gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg atcatattga
    46681 catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa
    46741 cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt
    46801 catgatacag ttggttcaag gaagtagcga gctttccact tcctgggcca ggagctgtca
    46861 ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca tcttctgaga
    46921 cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag accttgatgt
    46981 ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta tagcgagtga
    47041 taacgacact gttgattaat aagccataac cacgtaattc atcgattaac cgcaagatat
    47101 ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata tctccagcat
    47161 agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg attttcgcat
    47221 cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactagc ttcccaccaa
    47281 attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct gactgctctt
    47341 caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct taagtactaa
    47401 agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac
    47461 aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga tagtttttga
    47521 taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa acactatatc
    47581 cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga acaattgacg
    47641 tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg cattcatttc
    47701 acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga ttcgattctg
    47761 tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc
    47821 ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa aatggtacta
    47881 ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta tgaaagagcc
    47941 agatacaaat gcaaattatg agccagacga actagaggag acgaatgcgg atcaggatac
    48001 aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga atctttccca
    48061 attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata tccctgagat
    48121 ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa atgaaaatca
    48181 cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg aatttccttt
    48241 tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg gcacttatta
    48301 ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg ttttgcctaa
    48361 gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg acaaaaaaat
    48421 caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt tagatgaaca
    48481 aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat tcttgaatcg
    48541 taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac tatctaatga
    48601 aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc aaaaagaagc
    48661 attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag
    48721 gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc atgaattctt
    48781 gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag gttccgagta
    48841 tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa gatcatgaag
    48901 aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc
    48961 ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg aatacgaata
    49021 ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag
    49081 agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt
    49141 tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa tcatatgatc
    49201 gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg atcgaatact
    49261 tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca aattcagctt
    49321 atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga caaaacgaat
    49381 tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc
    49441 tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg gtgctttctt
    49501 tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg atattcaatc
    49561 acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt aatcagacat
    49621 tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa ttgacgaatg
    49681 aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga
    49741 tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg ataacaacaa
    49801 acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga tctgtcattg
    49861 atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc tgattggata
    49921 agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg gcagttatcc
    49981 agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa cttgctgatg
    50041 aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc ttattgtctg
    50101 ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag ccaatgactg
    50161 ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc
    50221 tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc ggccaatgga
    50281 tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa
    50341 ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac ggaataggta
    50401 tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat taccttcaag
    50461 gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt gtttttctgc
    50521 cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt
    50581 ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac ctatacacaa
    50641 tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa
    50701 gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg
    50761 agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa
    50821 gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga cgggaaaagt
    50881 gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat tttcaatttg
    50941 tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata attcagtgag
    51001 ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg aatgttttcc
    51061 gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt cggctatgca
    51121 atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga ttgcagcaat
    51181 cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga gttgcatcaa
    51241 tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat gcttttcttc
    51301 ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc atcccatgaa
    51361 gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat cgaaggaagt
    51421 tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact ttccacgatg
    51481 cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga agacttgtgt
    51541 gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc aaccgggatt
    51601 gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg agccgccatt
    51661 ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc aagcgattta
    51721 tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga tataaaaaca
    51781 gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt tggtaacaaa
    51841 gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca gtctcttcct
    51901 gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta ttctcgtaaa
    51961 taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg tttttaaata
    52021 aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt tgttgtaatt
    52081 aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca
    52141 gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt attgttgcaa
    52201 aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt ttccggtggc
    52261 gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac gacattattg
    52321 atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc cggttttatg
    52381 aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga tgaaaactat
    52441 gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa ggctttatat
    52501 gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc
    52561 tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat gttcattttc
    52621 atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt ctcaggcgta
    52681 ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc agcaacagtc
    52741 attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa agctaagaaa
    52801 ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga acaggaggcc
    52861 aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca tcgtggcata
    52921 tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat cctggttcag
    52981 tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta aagatgtttt
    53041 cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa tgattctagt
    53101 agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag gacttgcgtc
    53161 tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga gattgcgaca
    53221 taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct ggatgcaagt
    53281 ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt ttttatttta
    53341 ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa gatgtttatt
    53401 ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag gaacattttg
    53461 gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga ttccctggct
    53521 aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata atgttggcta
    53581 tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa
    53641 aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga tttttagaag
    53701 tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt atttatttgc
    53761 tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac tagctatcag
    53821 aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag gatatttcca
    53881 aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg ctgtgcgagt
    53941 gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa ccatattaga
    54001 agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga ttatccatat
    54061 gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga tttccagccg
    54121 ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa cactaccgtt
    54181 tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc
    54241 actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct tattcagccg
    54301 tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag gaacagtagc
    54361 tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa
    54421 aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat tgactggaat
    54481 gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg aaagtgtcgg
    54541 atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga tcctatctac
    54601 gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag aagcaattat
    54661 cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg ggatgagctt
    54721 atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga
    54781 ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt tccgctccac
    54841 cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc gattgtcaat
    54901 ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg gcggcttagt
    54961 atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca
    55021 tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa aaacggcaac
    55081 tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat ctaatcttac
    55141 ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta aacacatgaa
    55201 acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga accatcccga
    55261 cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg aaggaatcga
    55321 tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc ctactacttc
    55381 acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga aagggtatga
    55441 cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca cattatttgc
    55501 gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga tcactagtat
    55561 gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc agtcccttga
    55621 tgaaatactt gcacatatcg atcgaatccg agataatacc tacgcttatc tgattgtaga
    55681 tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc tgattggcag
    55741 cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa
    55801 aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta gaagaaataa
    55861 cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag aagtagcgat
    55921 tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt
    55981 cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca tttcagcgca
    56041 ataattcctt ttattcaaaa aacataccaa aaatattcca acatcatctt ggactttcta
    56101 catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat caatgaagct
    56161 ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt tagtaaatga
    56221 gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa aataatggaa
    56281 ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga taaagttgat
    56341 ggaagaaatg cattaaatac ggatgcaaaa gggctttcac tgggccttgc tcagtggaat
    56401 gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga aaaatggtca
    56461 cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt agctgcacaa
    56521 ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc agaaaatcca
    56581 gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga taccaaaaat
    56641 ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga cggagaactg
    56701 attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata ttaatagaca
    56761 gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa aaccgaacct
    56821 tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg acaagtgcag
    56881 aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa atgaaaatca
    56941 gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc aaagcactat
    57001 ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg gacaaatcag
    57061 atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac caagaattac
    57121 tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc cagaaccatt
    57181 acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa aggatcgaag
    57241 ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt cgagcgattg
    57301 aaaaaggata tttattagag attcaagagc cgactgtcat ccgagatgcc tctgttcttg
    57361 tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca ggaatcattc
    57421 gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa ggaaatcgcc
    57481 cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat cttcctgcct
    57541 tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa gaacttctct
    57601 taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac gccatcaaag
    57661 gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa ggacaagatt
    57721 tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca gatgaattgg
    57781 caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag gacatattga
    57841 gaaaagaaaa atggggtcat cctcaatctg agcatacaaa aggaagaacc attagcgccg
    57901 aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc ttagatgaaa
    57961 agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa gaaacaatag
    58021 aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc gaaggaaaaa
    58081 atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct gacatggaag
    58141 cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc atgaaggaaa
    58201 cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga aaaacgccgg
    58261 aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc cggcaaattt
    58321 tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac gaaggtcagc
    58381 ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa aagaacccgc
    58441 cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg tcaatgatac
    58501 gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa ttttctaaga
    58561 aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca agagaaaaga
    58621 cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat gaaaaattag
    58681 tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta gcagcagaaa
    58741 aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac ggtcagccaa
    58801 aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct gttttgaaag
    58861 aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac aaagaagaaa
    58921 tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat gaatttccca
    58981 agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa cgaacgcact
    59041 tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact tctaaaatag
    59101 aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac aagaacaaaa
    59161 atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag atagcaatgg
    59221 cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga aagatcttgg
    59281 tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata atggttatga
    59341 cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt ttgatttatt
    59401 gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca tcaaccatag
    59461 ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc cgtatcgcga
    59521 ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga catcgatttt
    59581 cggcggttct gcttgggagt attctcatga aactggacag tattatctgc atgtttttgc
    59641 aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac tattcaatat
    59701 gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga tttctcatgt
    59761 aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct ttcaaaatgt
    59821 ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt acgatatcat
    59881 gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg tagggaaaga
    59941 cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc aagaaaaaga
    60001 agggcaatta gatgtcctta aattgaaact tgcattaagc gcttggcaaa cttcattgga
    60061 tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg ccgtttcagt
    60121 gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg ctatgatgta
    60181 tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa tgacgaatat
    60241 gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt ataaaaggct
    60301 gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa caacgagaga
    60361 caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt ctacacatga
    60421 accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac aagaatatga
    60481 accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa ccaataaagg
    60541 tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt atgtctatga
    60601 aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat cacttgctga
    60661 ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa attcctctag
    60721 cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat ataaaacaaa
    60781 caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt ttttcagatt
    60841 gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat ttaggacgag
    60901 agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg acaggtactt
    60961 ggacatgttt gatatgcata ccgattgatg tgtcaccgat atcgataccg gcttgtccaa
    61021 ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca gaacaagcac
    61081 ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct tctgccactg
    61141 ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct agcgcaattt
    61201 ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact tccgcactgg
    61261 aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa acaaaaagat
    61321 cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc aattggtctt
    61381 ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta atattgcttc
    61441 cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat tccaatcaga
    61501 ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata taaaatccaa
    61561 ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc taatccccac
    61621 atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc gtggatcact
    61681 aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag accactggaa
    61741 gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata gatcccggcc
    61801 tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt taatgaaagg
    61861 gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt catttctcat
    61921 tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca tcagcacgcc
    61981 attagaaata caatgggcaa caaatttttt actttctgct actgctttgg taaaatctgc
    62041 ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc cgtttactgt
    62101 tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat agagaagatc
    62161 cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt ttgttcctga
    62221 agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa gagcttgacc
    62281 agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg gaaaaagttc
    62341 ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt tgaatgccaa
    62401 aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc gaacaaccat
    62461 gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag tcattttatc
    62521 aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca taaatcgctc
    62581 attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac caaaattctc
    62641 aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac cgccaattgt
    62701 caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat tttcaaaaaa
    62761 gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa aaagaaaaga
    62821 aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt ttaattcttt
    62881 ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat caaaatcacg
    62941 gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc gattccaatc
    63001 catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt tatcccttac
    63061 aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa tagctaatgt
    63121 actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt ggccatgctt
    63181 atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt cctggaaccg
    63241 tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta tatgaccatc
    63301 tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg tcatgtcttc
    63361 cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg tatgagcctt
    63421 aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg catcctgtgc
    63481 catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa aagtggagtg
    63541 atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac gccactgaac
    63601 aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca tatgaacaac
    63661 ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca tcaaagaaga
    63721 gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta atttcttttc
    63781 cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag aatgagcaga
    63841 atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat aagaaatttc
    63901 atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta aataaagaat
    63961 agga
//
LOCUS       DAZUJO010000007        61702 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000007 DAZUJO010000000
VERSION     DAZUJO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.007"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..499)
                     /locus_tag="ACG8OL_000488"
     CDS             complement(122..499)
                     /locus_tag="ACG8OL_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816501.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /gene="purC"
                     /locus_tag="ACG8OL_000489"
     CDS             755..1483
                     /gene="purC"
                     /locus_tag="ACG8OL_000489"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS4816502.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="ACG8OL_000490"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="ACG8OL_000490"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS4816503.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="ACG8OL_000491"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="ACG8OL_000491"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS4816504.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="ACG8OL_000492"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="ACG8OL_000492"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS4816505.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /gene="purF"
                     /locus_tag="ACG8OL_000493"
     CDS             4618..6057
                     /gene="purF"
                     /locus_tag="ACG8OL_000493"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS4816506.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /gene="purM"
                     /locus_tag="ACG8OL_000494"
     CDS             6080..7132
                     /gene="purM"
                     /locus_tag="ACG8OL_000494"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS4816507.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="ACG8OL_000495"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="ACG8OL_000495"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS4816508.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="ACG8OL_000496"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="ACG8OL_000496"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS4816509.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="ACG8OL_000497"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="ACG8OL_000497"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS4816510.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="ACG8OL_000498"
     CDS             10651..11565
                     /locus_tag="ACG8OL_000498"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS4816511.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="ACG8OL_000499"
     CDS             complement(11698..13263)
                     /locus_tag="ACG8OL_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816512.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="ACG8OL_000500"
     CDS             complement(13256..14707)
                     /locus_tag="ACG8OL_000500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4816513.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="ACG8OL_000501"
     CDS             complement(14721..15737)
                     /locus_tag="ACG8OL_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4816514.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /gene="chvE"
                     /locus_tag="ACG8OL_000502"
     CDS             15957..17045
                     /gene="chvE"
                     /locus_tag="ACG8OL_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4816515.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /gene="mmsA"
                     /locus_tag="ACG8OL_000503"
     CDS             17099..18625
                     /gene="mmsA"
                     /locus_tag="ACG8OL_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4816516.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /gene="mmsB"
                     /locus_tag="ACG8OL_000504"
     CDS             18627..19832
                     /gene="mmsB"
                     /locus_tag="ACG8OL_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS4816517.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="ACG8OL_000505"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="ACG8OL_000505"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS4816518.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="ACG8OL_000506"
     CDS             21330..22754
                     /locus_tag="ACG8OL_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS4816519.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22796..23053)
                     /locus_tag="ACG8OL_000507"
     CDS             complement(22796..23053)
                     /locus_tag="ACG8OL_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816520.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="ACG8OL_000508"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="ACG8OL_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS4816521.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="ACG8OL_000509"
     CDS             24327..25304
                     /locus_tag="ACG8OL_000509"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS4816522.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="ACG8OL_000510"
     CDS             25329..26972
                     /locus_tag="ACG8OL_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS4816523.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="ACG8OL_000511"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="ACG8OL_000511"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS4816524.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="ACG8OL_000512"
     CDS             28548..28823
                     /locus_tag="ACG8OL_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS4816525.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="ACG8OL_000513"
     CDS             28829..29596
                     /locus_tag="ACG8OL_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS4816526.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="ACG8OL_000514"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="ACG8OL_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS4816527.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="ACG8OL_000515"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="ACG8OL_000515"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS4816528.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="ACG8OL_000516"
     CDS             33740..34042
                     /locus_tag="ACG8OL_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS4816529.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="ACG8OL_000517"
     CDS             34045..35208
                     /locus_tag="ACG8OL_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4816530.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="ACG8OL_000518"
     CDS             35234..38662
                     /locus_tag="ACG8OL_000518"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS4816531.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="ACG8OL_000519"
     CDS             38683..39807
                     /locus_tag="ACG8OL_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS4816532.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39788..40234
                     /locus_tag="ACG8OL_000520"
     CDS             39788..40234
                     /locus_tag="ACG8OL_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4816533.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="ACG8OL_000521"
     CDS             40218..40556
                     /locus_tag="ACG8OL_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS4816534.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="ACG8OL_000522"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="ACG8OL_000522"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS4816535.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="ACG8OL_000523"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="ACG8OL_000523"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS4816536.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="ACG8OL_000524"
     CDS             41713..42756
                     /locus_tag="ACG8OL_000524"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS4816537.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42915..43643
                     /locus_tag="ACG8OL_000525"
     CDS             42915..43643
                     /locus_tag="ACG8OL_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS4816538.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43759..44004
                     /locus_tag="ACG8OL_000526"
     CDS             43759..44004
                     /locus_tag="ACG8OL_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4816539.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44001..44219
                     /locus_tag="ACG8OL_000527"
     CDS             44001..44219
                     /locus_tag="ACG8OL_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS4816540.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44355..44894
                     /locus_tag="ACG8OL_000528"
     CDS             44355..44894
                     /locus_tag="ACG8OL_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS4816541.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44887..47160
                     /locus_tag="ACG8OL_000529"
     CDS             44887..47160
                     /locus_tag="ACG8OL_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS4816542.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47198..48244
                     /gene="holA"
                     /locus_tag="ACG8OL_000530"
     CDS             47198..48244
                     /gene="holA"
                     /locus_tag="ACG8OL_000530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS4816543.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48333..48587)
                     /gene="rpsT"
                     /locus_tag="ACG8OL_000531"
     CDS             complement(48333..48587)
                     /gene="rpsT"
                     /locus_tag="ACG8OL_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS4816544.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48987..49256)
                     /locus_tag="ACG8OL_000532"
     CDS             complement(48987..49256)
                     /locus_tag="ACG8OL_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816545.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49518..49646)
                     /locus_tag="ACG8OL_000533"
     CDS             complement(49518..49646)
                     /locus_tag="ACG8OL_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816546.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49726..50181)
                     /locus_tag="ACG8OL_000534"
     CDS             complement(49726..50181)
                     /locus_tag="ACG8OL_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS4816547.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50319..52247)
                     /locus_tag="ACG8OL_000535"
     CDS             complement(50319..52247)
                     /locus_tag="ACG8OL_000535"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4816548.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52444..53277)
                     /locus_tag="ACG8OL_000536"
     CDS             complement(52444..53277)
                     /locus_tag="ACG8OL_000536"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS4816549.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53469..54173)
                     /locus_tag="ACG8OL_000537"
     CDS             complement(53469..54173)
                     /locus_tag="ACG8OL_000537"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4816550.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54196..54822)
                     /locus_tag="ACG8OL_000538"
     CDS             complement(54196..54822)
                     /locus_tag="ACG8OL_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4816551.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55148..57523
                     /locus_tag="ACG8OL_000539"
     CDS             55148..57523
                     /locus_tag="ACG8OL_000539"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS4816552.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57547..58038)
                     /locus_tag="ACG8OL_000540"
     CDS             complement(57547..58038)
                     /locus_tag="ACG8OL_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS4816553.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58225..59145
                     /locus_tag="ACG8OL_000541"
     CDS             58225..59145
                     /locus_tag="ACG8OL_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4816554.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59299..59643
                     /locus_tag="ACG8OL_000542"
     CDS             59299..59643
                     /locus_tag="ACG8OL_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS4816555.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59833..60333)
                     /locus_tag="ACG8OL_000543"
     CDS             complement(59833..60333)
                     /locus_tag="ACG8OL_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4816556.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60861..60944
                     /locus_tag="ACG8OL_000544"
     tRNA            60861..60944
                     /locus_tag="ACG8OL_000544"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60895..60897,aa:Leu,seq:cag)
     gene            complement(61268..>61702)
                     /locus_tag="ACG8OL_000545"
     CDS             complement(61268..>61702)
                     /locus_tag="ACG8OL_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4816557.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20233 a        10662 c        13216 g        17591 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    42781 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    42841 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    42901 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    42961 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    43021 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    43081 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    43141 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    43201 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    43261 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    43321 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    43381 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    43441 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    43501 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    43561 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    43621 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    43681 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    43741 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    43801 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    43861 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    43921 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    43981 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    44041 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    44101 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    44161 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    44221 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    44281 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    44341 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    44401 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    44461 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    44521 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    44581 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    44641 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    44701 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    44761 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    44821 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    44881 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    44941 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    45001 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    45061 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    45121 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    45181 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    45241 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    45301 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    45361 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    45421 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    45481 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    45541 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    45601 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga
    45661 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    45721 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    45781 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    45841 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    45901 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    45961 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    46021 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    46081 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    46141 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    46201 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    46261 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    46321 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    46381 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    46441 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    46501 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    46561 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    46621 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    46681 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    46741 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    46801 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    46861 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    46921 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    46981 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    47041 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    47101 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    47161 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    47221 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    47281 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    47341 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    47401 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    47461 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    47521 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    47581 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    47641 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    47701 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    47761 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    47821 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    47881 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    47941 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    48001 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    48061 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    48121 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    48181 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    48241 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    48301 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    48361 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    48421 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    48481 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    48541 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    48601 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    48661 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    48721 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    48781 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    48841 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    48901 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    48961 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    49021 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    49081 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    49141 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    49201 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    49261 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    49321 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    49381 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    49441 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    49501 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    49561 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    49621 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    49681 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    49741 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    49801 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    49861 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    49921 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    49981 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    50041 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    50101 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    50161 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    50221 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    50281 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    50341 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    50401 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    50461 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    50521 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    50581 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    50641 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    50701 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    50761 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    50821 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    50881 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    50941 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    51001 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    51061 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    51121 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    51181 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    51241 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    51301 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    51361 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    51421 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    51481 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    51541 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    51601 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    51661 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    51721 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    51781 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    51841 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    51901 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    51961 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    52021 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    52081 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    52141 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    52201 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    52261 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    52321 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    52381 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    52441 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    52501 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    52561 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    52621 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    52681 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    52741 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    52801 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    52861 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    52921 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    52981 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    53041 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    53101 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    53161 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    53221 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    53281 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    53341 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    53401 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    53461 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    53521 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    53581 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    53641 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    53701 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    53761 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    53821 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    53881 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    53941 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    54001 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    54061 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    54121 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    54181 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    54241 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    54301 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    54361 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    54421 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    54481 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    54541 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    54601 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    54661 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    54721 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    54781 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    54841 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    54901 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    54961 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    55021 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    55081 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    55141 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    55201 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    55261 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    55321 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    55381 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    55441 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    55501 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    55561 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    55621 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    55681 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    55741 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    55801 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    55861 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    55921 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    55981 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    56041 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    56101 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    56161 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    56221 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    56281 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    56341 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    56401 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    56461 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    56521 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    56581 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    56641 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    56701 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    56761 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    56821 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    56881 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    56941 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    57001 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    57061 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    57121 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    57181 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    57241 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    57301 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    57361 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    57421 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    57481 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    57541 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    57601 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    57661 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    57721 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    57781 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    57841 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    57901 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    57961 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    58021 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    58081 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    58141 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    58201 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    58261 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    58321 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    58381 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    58441 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    58501 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    58561 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    58621 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    58681 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    58741 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    58801 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    58861 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    58921 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    58981 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    59041 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    59101 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    59161 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    59221 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    59281 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    59341 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    59401 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    59461 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    59521 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    59581 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    59641 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    59701 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    59761 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    59821 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    59881 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    59941 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    60001 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    60061 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    60121 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    60181 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    60241 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    60301 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    60361 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    60421 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    60481 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    60541 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    60601 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    60661 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    60721 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    60781 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    60841 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    60901 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    60961 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    61021 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    61081 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    61141 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    61201 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata
    61261 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
    61321 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
    61381 ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag
    61441 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag
    61501 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt
    61561 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg
    61621 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    61681 ttgttaattg ttgtcctgag ta
//
LOCUS       DAZUJO010000008        55288 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000008 DAZUJO010000000
VERSION     DAZUJO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.008"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..325)
                     /locus_tag="ACG8OL_000546"
     CDS             complement(<1..325)
                     /locus_tag="ACG8OL_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816558.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKI"
     gene            complement(332..880)
                     /locus_tag="ACG8OL_000547"
     CDS             complement(332..880)
                     /locus_tag="ACG8OL_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS4816559.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(925..1257)
                     /locus_tag="ACG8OL_000548"
     CDS             complement(925..1257)
                     /locus_tag="ACG8OL_000548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816560.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1271..1528)
                     /locus_tag="ACG8OL_000549"
     CDS             complement(1271..1528)
                     /locus_tag="ACG8OL_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816561.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1723..2265)
                     /locus_tag="ACG8OL_000550"
     CDS             complement(1723..2265)
                     /locus_tag="ACG8OL_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFS4816562.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2286..3377)
                     /locus_tag="ACG8OL_000551"
     CDS             complement(2286..3377)
                     /locus_tag="ACG8OL_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4816563.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3385..5595)
                     /locus_tag="ACG8OL_000552"
     CDS             complement(3385..5595)
                     /locus_tag="ACG8OL_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4816564.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5611..8139)
                     /locus_tag="ACG8OL_000553"
     CDS             complement(5611..8139)
                     /locus_tag="ACG8OL_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4816565.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8191..8601)
                     /locus_tag="ACG8OL_000554"
     CDS             complement(8191..8601)
                     /locus_tag="ACG8OL_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS4816566.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8614..8832)
                     /locus_tag="ACG8OL_000555"
     CDS             complement(8614..8832)
                     /locus_tag="ACG8OL_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFS4816567.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8829..9821)
                     /locus_tag="ACG8OL_000556"
     CDS             complement(8829..9821)
                     /locus_tag="ACG8OL_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4816568.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9900..10811)
                     /locus_tag="ACG8OL_000557"
     CDS             complement(9900..10811)
                     /locus_tag="ACG8OL_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS4816569.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10830..11174)
                     /locus_tag="ACG8OL_000558"
     CDS             complement(10830..11174)
                     /locus_tag="ACG8OL_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816570.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11194..11472)
                     /locus_tag="ACG8OL_000559"
     CDS             complement(11194..11472)
                     /locus_tag="ACG8OL_000559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816571.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11831..12331)
                     /locus_tag="ACG8OL_000560"
     CDS             complement(11831..12331)
                     /locus_tag="ACG8OL_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816572.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12332..12877)
                     /locus_tag="ACG8OL_000561"
     CDS             complement(12332..12877)
                     /locus_tag="ACG8OL_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816573.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12874..14130)
                     /locus_tag="ACG8OL_000562"
     CDS             complement(12874..14130)
                     /locus_tag="ACG8OL_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4816574.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14491..14802)
                     /locus_tag="ACG8OL_000563"
     CDS             complement(14491..14802)
                     /locus_tag="ACG8OL_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816575.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14825..16300)
                     /locus_tag="ACG8OL_000564"
     CDS             complement(14825..16300)
                     /locus_tag="ACG8OL_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS4816576.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16749..17201)
                     /locus_tag="ACG8OL_000565"
     CDS             complement(16749..17201)
                     /locus_tag="ACG8OL_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816577.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17203..17568)
                     /locus_tag="ACG8OL_000566"
     CDS             complement(17203..17568)
                     /locus_tag="ACG8OL_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816578.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17667..21950)
                     /locus_tag="ACG8OL_000567"
     CDS             complement(17667..21950)
                     /locus_tag="ACG8OL_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFS4816579.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22097..22336)
                     /locus_tag="ACG8OL_000568"
     CDS             complement(22097..22336)
                     /locus_tag="ACG8OL_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816580.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22426..22668)
                     /locus_tag="ACG8OL_000569"
                     /pseudo
     CDS             complement(22426..22668)
                     /locus_tag="ACG8OL_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22781..23017)
                     /locus_tag="ACG8OL_000570"
     CDS             complement(22781..23017)
                     /locus_tag="ACG8OL_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816581.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(24028..24938)
                     /locus_tag="ACG8OL_000571"
                     /pseudo
     CDS             complement(24028..24938)
                     /locus_tag="ACG8OL_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(25134..26969)
                     /gene="lepA"
                     /locus_tag="ACG8OL_000572"
     CDS             complement(25134..26969)
                     /gene="lepA"
                     /locus_tag="ACG8OL_000572"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS4816582.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            27116..28336
                     /locus_tag="ACG8OL_000573"
     CDS             27116..28336
                     /locus_tag="ACG8OL_000573"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4816583.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28356..28850
                     /locus_tag="ACG8OL_000574"
     CDS             28356..28850
                     /locus_tag="ACG8OL_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4816584.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            28828..29514
                     /locus_tag="ACG8OL_000575"
     CDS             28828..29514
                     /locus_tag="ACG8OL_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816585.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(29665..32274)
                     /gene="clpB"
                     /locus_tag="ACG8OL_000576"
     CDS             complement(29665..32274)
                     /gene="clpB"
                     /locus_tag="ACG8OL_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS4816586.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(32525..33571)
                     /locus_tag="ACG8OL_000577"
     CDS             complement(32525..33571)
                     /locus_tag="ACG8OL_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS4816587.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(33582..33854)
                     /locus_tag="ACG8OL_000578"
     CDS             complement(33582..33854)
                     /locus_tag="ACG8OL_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS4816588.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(33889..34797)
                     /locus_tag="ACG8OL_000579"
     CDS             complement(33889..34797)
                     /locus_tag="ACG8OL_000579"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4816589.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(34929..36161)
                     /gene="pepT"
                     /locus_tag="ACG8OL_000580"
     CDS             complement(34929..36161)
                     /gene="pepT"
                     /locus_tag="ACG8OL_000580"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS4816590.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(36176..37297)
                     /locus_tag="ACG8OL_000581"
     CDS             complement(36176..37297)
                     /locus_tag="ACG8OL_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS4816591.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(37294..37995)
                     /locus_tag="ACG8OL_000582"
     CDS             complement(37294..37995)
                     /locus_tag="ACG8OL_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS4816592.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(38139..38579)
                     /locus_tag="ACG8OL_000583"
     CDS             complement(38139..38579)
                     /locus_tag="ACG8OL_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS4816593.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(38708..39634)
                     /gene="dnaI"
                     /locus_tag="ACG8OL_000584"
     CDS             complement(38708..39634)
                     /gene="dnaI"
                     /locus_tag="ACG8OL_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS4816594.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(39634..41007)
                     /locus_tag="ACG8OL_000585"
     CDS             complement(39634..41007)
                     /locus_tag="ACG8OL_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4816595.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(41030..41521)
                     /gene="nrdR"
                     /locus_tag="ACG8OL_000586"
     CDS             complement(41030..41521)
                     /gene="nrdR"
                     /locus_tag="ACG8OL_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS4816596.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(41833..42165)
                     /locus_tag="ACG8OL_000587"
     CDS             complement(41833..42165)
                     /locus_tag="ACG8OL_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4816597.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(42217..42846)
                     /gene="coaE"
                     /locus_tag="ACG8OL_000588"
     CDS             complement(42217..42846)
                     /gene="coaE"
                     /locus_tag="ACG8OL_000588"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS4816598.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            complement(42810..43646)
                     /gene="mutM"
                     /locus_tag="ACG8OL_000589"
     CDS             complement(42810..43646)
                     /gene="mutM"
                     /locus_tag="ACG8OL_000589"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS4816599.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(43691..46336)
                     /gene="polA"
                     /locus_tag="ACG8OL_000590"
     CDS             complement(43691..46336)
                     /gene="polA"
                     /locus_tag="ACG8OL_000590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS4816600.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(46462..47145)
                     /locus_tag="ACG8OL_000591"
     CDS             complement(46462..47145)
                     /locus_tag="ACG8OL_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS4816601.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(47138..47623)
                     /locus_tag="ACG8OL_000592"
     CDS             complement(47138..47623)
                     /locus_tag="ACG8OL_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS4816602.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(47930..49264)
                     /gene="murC"
                     /locus_tag="ACG8OL_000593"
     CDS             complement(47930..49264)
                     /gene="murC"
                     /locus_tag="ACG8OL_000593"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS4816603.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(49395..50156)
                     /locus_tag="ACG8OL_000594"
     CDS             complement(49395..50156)
                     /locus_tag="ACG8OL_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4816604.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(50172..50654)
                     /locus_tag="ACG8OL_000595"
     CDS             complement(50172..50654)
                     /locus_tag="ACG8OL_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS4816605.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(50674..52854)
                     /locus_tag="ACG8OL_000596"
     CDS             complement(50674..52854)
                     /locus_tag="ACG8OL_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS4816606.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(52998..53729)
                     /locus_tag="ACG8OL_000597"
     CDS             complement(52998..53729)
                     /locus_tag="ACG8OL_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4816607.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(53798..54739)
                     /locus_tag="ACG8OL_000598"
     CDS             complement(53798..54739)
                     /locus_tag="ACG8OL_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS4816608.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(54919..55287)
                     /locus_tag="ACG8OL_000599"
     CDS             complement(54919..55287)
                     /locus_tag="ACG8OL_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS4816609.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT        15599 a        11897 c         8995 g        18797 t
ORIGIN      
        1 taatttttaa acaaaattct ccccaaaaag gaactttttc tagtgaaaac ttggtaatct
       61 ctttgtaatt aattcttgtt aaagaataat taaacacttc atttttactt gttatttttt
      121 cattgactat aactagttca tctggtaaaa aaattaacca acaaacatcg acaaacagta
      181 aatgatataa tccatttcca cctccaagca tattcaatac tggtgaacat gccttagaaa
      241 cacgaatata gttatggttt aaatagatat tttctttaaa cattgcttgt ttaaaagcca
      301 tatgctcttg ttctaaactt ttcataaact tttactctaa tgcacagcta gcagcagctg
      361 ctgcaattcc tgctacaata tttaccttag ctaactcttt agctgcagct tttcctaatt
      421 gtttttctgc aatttttaat aataaatccg cagctttttc aaaattaaaa gcttttaatg
      481 cttcaataaa atctccctgc gtacctatag cccaagccac tcctcctcca ggaagagaat
      541 taatggctac acatccagca tatcgtttcc aatcaacttt ctccattcta ggaacaataa
      601 tatgttcata aaagactctt gctgctcttc cttgttcgtt atcttgactg attcgttcac
      661 gaatagcacc aaaatccttt atatggtata caccatgttc gtctaaaaaa ccaactgtat
      721 caaaataaaa agttaattca tctactaatt tattctttat ttctaaatca actgtattca
      781 ctttttctgt ggcatacaca cttgttccag tactaacaaa agtacttaaa cctaataaga
      841 aaacacctaa aacaccaaat attttaatta ttcttttcat aatttctcct ccataaaaaa
      901 taagttactt tttaaatatc ttgtttatga attcattccc caaaggcctt ttaagattga
      961 tacagcagtc cgttcacttc ttttagactt gtcatacaag tcttttaaat gatcccatat
     1021 cagactagtt tgtctatcta gcttcaatcc attaaacgct aatgaacggt tcattttttc
     1081 tattacttta gataaagcta cagaaatttt tataccttca caacgcgcat cttggatttc
     1141 aataatagta tcatcaatta gtttttgaat ttcttgccta tcaacatatt caatcttaga
     1201 aagagaatct tttaaagcta ctaatgattc aacgatttct tttttctctt cagacatttt
     1261 cttcctcctt ctagtgaccc gtaaagccat gtagagcaga gtccattgaa ccagtaatta
     1321 cactatctac aacgccattc caatttactc tacattttcc atttttcaga tttgtcggtc
     1381 catacggata gcctttttga cattcaatgt tcggattata ccagatagga ttataccatt
     1441 cagccgcaga agcagattca gtcgatacaa acaatcccac accaacaaat ccaatacaca
     1501 tcacactata gccaattagt tttttcatct cgtatccatc tcctaaaaaa tagaatagtt
     1561 cttatagtta ttcataagac atattttttc cgtttttgtt ttatcctttc tgtcctataa
     1621 caactttttt caagtaaaga atgacatagt ttttcttaaa aaaacagtct gtttttttct
     1681 atttcttcgg tgtataataa atatgagtgt tcatttttta ttttaatacg cactccgtac
     1741 ataataaatc ttgatcgcat caatcttttg agcttcttga tctactttca attgaagtaa
     1801 tacaaattga gtaaccttat tcattgtttc ttctccaaat aacaaatatt gatccggatt
     1861 ctctatatct tgtgtcacct ggtaaagttt cccagttgtt ttcatttgta catgcgggtc
     1921 tacatcaata ctattgtctt taatacaacg atctgtcata tagtccctca cagcttgatt
     1981 gcgtttataa acatctgtga aattactcca ttttctagca aaatctttta agaaattttt
     2041 tgtcatttca tctatttgca tttgttgcgc tgtctcgtct tctaatgaac caatttgact
     2101 gttactttca gtactttgaa gagttccact tgtttctttt actgcttcga ttgtttcttc
     2161 tgtcgttctc aattgtgttt tttgtttttc agaagaaaac caatgacaac cagtaagacc
     2221 tatcaaacat aaccaactaa atactaaaat tatccctttt ttcacgtttc tagtcactgc
     2281 ctttcttatt tcaccttacg aaccagacta cgaatttttg tttgattgta cgtgcgttga
     2341 taccgagcca taatccgtcc ttgctcggca ttctgttcca cactagagaa atgttgtccg
     2401 ccatatgaaa catctgttat aattccggtg tgaccataga ttccaggaga caatgcacca
     2461 cctgcttgcc aattcaccac atctcctgct tgtaaatcac ttggttttgg gttcatgatt
     2521 acttgccaac cataggttgt ccagttataa tcttccccta ttaacatcgc aaacatgtgt
     2581 ccagaaccca ttaactgtgg gccacctaat tgagatacat accatgccgt accaccataa
     2641 cattccccat tatttacgga tcgtccaatg atccgatcca aaaccttcgc attgccttta
     2701 ctttttcctg caataagcga agtttttttt gttggtaggc tattccaatg cttgatcgca
     2761 aaagtatcat attccgttaa gttataggac gttatgatac tcattgctcc tcgataatac
     2821 ggactccctt cggtaatacc tgctgccgca tagcctccac gacctagaat caataaacaa
     2881 cttccaggat ctttattgcc cacacattga ttaaagttgc tttggctcgc caccataaag
     2941 ccataatagt cataacaagc ttttaagttc ggaaacttca tatagaaacc accttcagaa
     3001 ccaccaattc cacgtgcaga accttgtggg tagggacaac caggaaacca ggtaatccca
     3061 aaaaagttcg tatcgtttct tccagattgt gactgtccat aagctgattc atagcctaat
     3121 tgagaaagaa tcacactcgg taataactga tactttacag ctgcaccaac tgctgcttgc
     3181 gcatgttcat ctgtgattgg accacgtcct ttaatctctg aaaaaatcgg taatccttcg
     3241 gtatattccc cattaaaagc gacagctaga cctgtagctt ctcctccatg ctcagaaagt
     3301 tctcctgaaa aaaagaatgg caatagaaaa aagggaagaa taaagaaaag aagacaacct
     3361 agacaccctt tcttcatttc ttcctcatcc tttcttcgat aatggttttt ggactgttcg
     3421 tccttttggt aaagtaaaat cagttcctgg ttttgcgtcc aaaatcggca gtgttttagg
     3481 atccttttct attttttccg attgctctgt tggcttatct aaagaagaaa atgcaggttg
     3541 taacgatttt ggtgcggaaa tcgacggttt actttcagac gaacgttgcc ccggttgttg
     3601 cacagaacga tttcctgata gagtggctac cgacgaatta ggaacagagt attggttcaa
     3661 taactgctgc attgctggat aactcccatt tttcgcctga tttttcactt ggcgaatctg
     3721 ttgtgaaaga gaaccagcag ctgttaattg tggcacgggt acttttccag aaggcaacgg
     3781 aatcgattga ccaacaaaag cgataccttt atttaacaat ggttttcttt gattattccc
     3841 ttgttgctta ctcaattgtg ccaatgattg tacctgttgt tttggtctta ctgaattcat
     3901 ggacgaattt ggaatcaaac catttgaaag accagaattc cactctgttt tcgatttggt
     3961 agaagacatt tcaggctcta ccccatgata acgaacatga cgaccactat aaggcaaggc
     4021 ttgatttgaa cgcccaccta gttcaaaggg attaaccact tgcctccctt ttaccgattc
     4081 ttgtggtgta cgtttttgtt gaaacttttg aaatccatgt atcgctgcag aagcgagtgg
     4141 attcgttgct cgatacacat tccctgctaa tcctgcacct ttgattgcca aattttgtcc
     4201 agcagttgat agttgaccag gcagttgttt catttgagac aaagaatgac ttgctatgtt
     4261 tttcgtgttg tttatagttt gatctgcagc tcctaaccct agtttcttta ataaatcttt
     4321 ccgttttaca atgagtaaaa ttgataggag tgctttgaca aacatatttc ctaaaaaacc
     4381 aaaaacagta ttcattggaa ttaaatcgtc tactatgtta tagatcaaaa atgccaccaa
     4441 aataatcaca ctgtaaaaag atttttgaaa catcaagccc aaaaaggact ttaaggtatt
     4501 catgagcata taatcaaata ctggaacaaa agaagcgagt aatgaaaaag gaatcacaag
     4561 taataaacac aacaccaaca tttgaaagat aaaacgaatc atgccaatca ctacaagtgg
     4621 taatccaata cttagattaa gcaatggcac catagaacca acgactgctt tataaacagc
     4681 tttagttggt tgaatataga cacgataagc cttctttttc cctctatctt ctcctgcagc
     4741 agtggtcact tttgtctcaa tatcagataa ttcttcactt gacgcttcct tggctagaaa
     4801 ttcttgtggt tcaatctctg cttgatacaa ttcttctaaa tttgttgtcc cataatttaa
     4861 caatatatac ggatcaacaa cagccgcttt ataataaagt tggcgcactt tttgaatggc
     4921 ttccttttgt gtcgttggca ctaaaacacc attgacatct tttgtcgcca attcatctag
     4981 actatttgta gcagaaaaaa taaagccttc tacctccacg gacaaatcat ttaacccttt
     5041 ggtaaaagct tctcctttac taggtacgtt actaccatag ccaaaccaaa ccattgaaaa
     5101 aatgacaacg aagagaaatc gaataaacac catttttgcg taatgtttcc ctctaaatat
     5161 acgaagagta aatagatata acacatacaa gacgacaatc cccgcaccaa taggatcgta
     5221 tatttttgaa taaatagtat ccgaaaaagt gaaaaactgg tgaattaact cgttcattgc
     5281 agctccttca tagagcttct caatgacaaa atcaaggcct tgccaaatca atttacttaa
     5341 actaaagaac atattcgtca tcatttttag aaaatctgtg ccatttgccc caaataacat
     5401 tttgcctaat gaaaattcac tatcagataa ataagaagaa aaggtatcaa tatgaaattt
     5461 cccaatctca tagatcgatt gatccacctg cttttctgcc gcatacacca atccaccaac
     5521 aaaaagcagt ccgaatccaa tcgttaaaaa aaacaaaacg caccatttct tttccttatg
     5581 ctttatataa gccattgttt cacctcattt ctattgcatg attaacgtat cctgttcttc
     5641 tacttgagag tgacttgttt tttccgttgt ttgtaatgca gcggtcattt cttcaaacaa
     5701 actgtgtaca ttgagttttc ctacacgtcc atagggatct aacatccaac actgtccttt
     5761 aatcatgtgg ctcatttcag ctaagttcgc ttcatttact tccaaacgta aatgattcaa
     5821 aatcttttct cgctcactgt cttcatcaaa agcaaagacg acaccaaaat tccctttttc
     5881 tttatctgaa cctaagtcac caataaattg tgtatctaac actaattgat tcatctggga
     5941 acgccccaca cgtgccattg ctgcaataat actacgtcct gcgttcgaat tattaaatac
     6001 ccatgcttct gtaaaataaa tcgctgtttt cttactacta tctcgttttc caaacatttc
     6061 acaaaaacga cctaaactaa tcatcaaaca caaagacttt cgatccgcat ctgtatatag
     6121 ttctggtcgg acattgtcat ttggtaattt caaatcttgg atctctaaaa tcgttgtttt
     6181 ggcattaaag tctaatcccg ggttttctcc ataagaaaat cctaaacgta aaatcgaatc
     6241 ttctaccgtc aagcgtaaaa actcaccata ttctcgaacc tctttttgtt ccatttgttc
     6301 taaatagtcg accacatgca tcaagccaac ttgttgtccg gcttcttttt gaactaaaac
     6361 ttcttggatt ccctttaata ccgccccttt taaaaggtga ttggtaccta acggacttaa
     6421 ctcatcaatc atatcctggg ctacttgttt cgcatctact cgggataaac aaacaattgg
     6481 atctaataca ccataattac ggggatttgt agcatctaac gtgacataac tcattttttg
     6541 caaatgttct acaaacaaag ggtaatgttt ctgataatac gggtcttgaa tgagtttcgt
     6601 aaaccatttg cgcttttctc ctttgggatc cacaaataaa ctttgtacat tcataaacga
     6661 actatacaaa aagagcaagc caactaaaaa ggtcttccct ttaccggttt ttccagttac
     6721 tgctacatga ggcgcatcaa acaacgcacc tttgattcct tccgcaatgg ctagaagatt
     6781 caaaaagaca aaacgatttg acgcataaat taattcttct aatggtgttt tttctcctcg
     6841 agaaccacca tcccctaatg tatcttgccg accaatatac cagccggatt ccatacctaa
     6901 ttctgatact gctgaaaaca accattcgcc aactgcttct acagttgttc ggtgcttcca
     6961 atgtaactgc cgtcccaatg gttgccctaa taaacattta tagaacaatt ctcgttgatc
     7021 attttttgca cggtggatat ggataatcgt atgaagttgt tgaataaccg ttgcactatt
     7081 ttgacgaact tcttcaaccg tttgaccgta tacaataaaa catgcgatcc aatcgacgac
     7141 aggttgccca ttatctactc gttgctttaa atgttttact aatcctcgaa cagttaataa
     7201 gcgttcacta ggttcttcat cattttgaac caattcctta tgctctgttt tgaaccgttc
     7261 cgatagccaa ttcagtttta aaccaatacc tccctgtcct ttcaagcgtg gatagtgaat
     7321 tttcattcgc aattctgctg ggaaagaaag atcctgagca aattgaaata gatcacaatt
     7381 tctcatattt tcaggtgttt ccccaatagg caacaatgct aaataactac tccctaactc
     7441 atcttctaat tgaagaaggc ctttatgttc cttcggtata cgtacatacg ttccttgatt
     7501 gtctttcagt acttcccaat tattttgttc ttctactttg tgagccattc ctcgaatata
     7561 ttgcagacga ttgagataca acagctcttt ttttactaag ggacgaatag aaaagctata
     7621 aagtaattgt gtcacttctt ttacataagg atcactttca gaaaacaaag tttccaaatc
     7681 atctacttct aatccaaata agcgtgctaa acttcctgct aaatctaacc agattagatt
     7741 tattacatca tgaaaactct ccaccgttgc tttttctttg actttgattc ctaaaataaa
     7801 tgaaggacga tagacttgac cttctcgttc caacaatgtc actgtttctt ttgcataata
     7861 cgcaccaatt gcttgactat cgggagaaaa tccctttcct aatgtgtcaa acttcgtccg
     7921 taaattcatt gttcgtggat acatcaccaa ctccacatct tcataatctg ctaaaagcaa
     7981 cagtaaacga gccatcattt cttttatttc ctgtgtcttt tttggtgtgt tttttgaaat
     8041 tgttcgtgga aaaacatgat agtaggccca gatacaatcc tctccattta acaaacaatt
     8101 tttcttgaat gcttttactg gttcttttac aagcaccaat gaatttcacc tcccaataaa
     8161 aaaggaacaa ccagttgatt gtcccttttt ctaatcctct aaataaacat acttattcac
     8221 aaaaagtaat tcttccaatg cattgtttga taccttgtcc cagttataat acacacaatc
     8281 accattcatc atacgaatca tataacaaaa ccaatcccat aaataaaaat gcactttttt
     8341 tccatcaggt tttagttttg ccagtgctaa cacacataaa taggggagtc ctatataaaa
     8401 aacaactcct gctggtggaa aaaaagaaat gacccaacca ataaatgaac gtaaaaataa
     8461 atattcaaag attacgacca acaaaaaagt tgctgcattg actaatgcaa ctggaaatgg
     8521 aagacgccaa ttgcttgtaa tctgttgtac gcgataaggt tccgcataaa caatcgaata
     8581 atcataaggt tcccaatcca cttcactatc ctcctaatcc ataaataatt tttcaatgat
     8641 tttaccaaac gcatcaaata cacgtgttgg atcatttaag aagaaataaa ctgcggcacc
     8701 tgctgcgata gatattaatg catttcctac ttttgatttc ataaaatttc gcaatcccag
     8761 ataaacaacc accattatta aaaaatagcc accttgcgtt aatataaaat ctacaattcc
     8821 cttcagtgtc atttccgttc ttctcctttc ctatccaacc aattatgtgt taatttttca
     8881 acataatact gatttgtttt tttcgtaatg acaatcgtca tttgttcttt ttgtaaaagt
     8941 gacgtatcac catctcgaaa gacgaccgtt gcttttacaa taaaaggttt tttcgtttct
     9001 ttataacgca atactttcat ctctacttga tcaactttag ctgtttgatt gagtccttca
     9061 ggttctttca tcatgtatga catatctttt gcattgtttt tcgcatactt atctaagaaa
     9121 tcttccacaa atccttcaac atctttcgct tccgacgcat caaccgtttc gtataatgtg
     9181 tcatcataag taatcattcg ttgtttttct gtattctttg gtagtgccgt aaaataagga
     9241 taagcaataa tacgacagcc cttgtttgaa atagaaaagg gaagattgag tacttgtgtg
     9301 attgtcacac ttgtttcttt cttactctct ggattcatcg agcgaatatc ataagttact
     9361 ttataactag cagttggaat ggtttcaatt tcttttaatc caataaacga agcagcagtt
     9421 aattttcgaa cacctgaaac acttcctggt tcttgaataa agttttgtgc ataaaactct
     9481 ttttttaatg tttcttgacg ttgttgttct tgttcatgat tatcgaatgt attcatatac
     9541 aaagcaacaa aacgattcat aaaggattca atttcaggtg tgtacgttaa ttctgaccga
     9601 ctttgtactt ctttcttttg taattgttgg atttcatctt tcaaaaacga cacttgaacc
     9661 ttcgttttca tggattgaaa aaatgccaag ccccctgaaa aacaaagaaa taaaaccaaa
     9721 caccaaatcc atttcactgc tttttttgaa tggattttct tttgcttcgg tactttctct
     9781 gtataaactt tttctttccg ccgtagttgt cgttttttca taagctactc cttctagtat
     9841 gtttttacta gtacgtacga ttgacaacga taagcttgtt tttctgaaaa atagtccatt
     9901 caattaccct cattttctat tccttttctc tttcttttat atccaacaaa tccagcaata
     9961 cttgtcaaca ctcctcctag aacagaaagt gcaacatgta tttcttcacc agtctttggt
    10021 aagacttttg tttttccatc tgctgttttg atttcaatat tgccacttgg aagtttcttt
    10081 acaggaatcg acaaatcctt taccaaattc ccttcactat cttggattaa tggaatacca
    10141 tcttttacac caacaattgt ttgatcacct gctactttta atggggtggt tggtgttgga
    10201 atatgggtca attcctctac attactagtg acaatcggta cactcattcc ttgagaagta
    10261 ttcgtcgtaa tttcaccgct tggggttacc gatactgtga cttcttttgc tggaacaata
    10321 ggtttatttt cttttggaat tgcttctgat ttttcaacag gttgtttcgg ttcttctttt
    10381 tgttcttcac tggtagacga catcgttggc tgttccgtac ttgacgaatc agtattcttt
    10441 tcagaagaac tggttgttcc tgattgttcc ggagttgtga ctatacttga atccgttgtt
    10501 gttgaactat gcacaccttc ttcttcgatc gaatcagtcg gtaaagttga ttcaggaaac
    10561 tctggttctg gtgtcactgg ctctttttca ggtttctctg actgcaattg ttcaggttct
    10621 gctggttttt ccgttgttcc tccatttagc tctgtctcaa catctggtaa tccttcccct
    10681 ggcaatgttt ctacaatttc tgacttaggt gtttctacag tttcatctgc ataaaccaat
    10741 gaactcccta attgaaacga aaataatgaa attcctagtg ttgttacgac tgctaatgtt
    10801 gtttttttca tgattattcc tccaattttt tatgcaattc gtgttttgtt catagacgcc
    10861 gcactaatat tatctgaacg ataaaagaac tcccgtccgt tttcttctaa cccaacatat
    10921 ggattatttt tgaataattg aatcaatagt tcactggtgt tgtattttgg atagtttgca
    10981 aaaaaataat actgtgcttg tcgcccatcc gaaaattcaa caagtaattc ataacgatac
    11041 cgcttcaatc cttttcgttg ttttaaagga tctctatacc aacttgtcca tcgttccatt
    11101 cgtggataga gatagacacg atacacatag aacaatgcca taccacatac caccaaaaat
    11161 aataatcgcc acataaaaat tccccctttt cttctacctt tcttgaatca tcgaacattc
    11221 ataccctcta tagagatttg aaaaatccat catcaattgt tcatataaac gttgttgcaa
    11281 atagcgattg tttcgaatac ataattcttc atagccatca atcacttgat cttctttttc
    11341 atagcatctt gtagctctat gacgctcagc ttgcaagaca taaccaaact ctgcaccata
    11401 tcgttctact gttaataact gttgactacg caataatgca atataacaat cacaaaaatg
    11461 ttcaaccgac atttgatttt tctcacgttt gtgaaatagg tattctttag aaagcacatt
    11521 attcgttaaa caatctgata aatagcgata atctggcggt cggttatttt catctgtgaa
    11581 tttttcccat aataaactga tttttattcg ttcagcggct agatagtctt gttcgcgtgg
    11641 ataaaattca tcaatcgcac aaagtttttt atcacctgaa taaacaaatc catataatgc
    11701 gaaacgaact cttccttttc aattggctct cctaaaaatt ccagtgacat accatcttct
    11761 ccttcctaaa aaaaataaaa aagcagaaac cgatttggct tctacttttc tttcctttta
    11821 tagctctttt ttacggaaga atccgcaaat gacatttttt caaaaaatca atcggcacaa
    11881 gttcacgaaa acttggagca attttggata gaatcacttc cccatcttgt aaaatacaat
    11941 cataaaaatc atcagttact tgatacttac gcttcttaca ataattgata aaatagtagc
    12001 gatcattttc tatttccatt cgttgttcta ctaagcgcat acgaacatca tcatagaaat
    12061 aagccacaaa tcgcccttca agttcatgtt tttcaatggg atactcttct ggtaacaatc
    12121 tattttttcg tcgtttatac attgctggat accttagaag aagttcatct aaacgtctgt
    12181 cagtatatgg taactgacga atcaacgatt ctaatcgttc tttatacaga tagaataatt
    12241 ggcgcaagca atagtgtacc gcctctggat tttctaatac tgttggatca cgtgaccaat
    12301 acgcaccaac ataaatcata atttttttca tttagacaat cacaccagca atactaatga
    12361 ccgatccaca ataggtagaa caagtggcat agcgaccaat ttcctctaat tctatctcaa
    12421 tctcttctaa agaaagatat gtctgttctt ctgttggctg gcgactcatt tcccaagtga
    12481 cagtcaccaa tgaaatttct tccataagag gtttttcgct gctaagtgct gccacacagt
    12541 tttcatatat cttcatttta tcgcagcctt ttttaaccgc taaaatcgca ggtattggca
    12601 atgaaaatcg ataaaagcga atgaattcta gccttccttt ccttcggcta aattccaact
    12661 cttcgagttc ttgttcgtat cccttacgaa cttctaataa gcgtgcaatt tcttgccagt
    12721 tctctttttc ctgataacgt ccagctttta ccgcttgttc aatataagga actcgaaaac
    12781 gaaaataaga caaatcaacc atcttttcat ttaatgcttc gattgcagcc tttaaatcca
    12841 acaggcgcag ccattctgtt gtcgtatatg cattcatgca attttccctc ctgacgttcc
    12901 catccattct agatagtttt gttgctcgtc atttagttcg gcattatcaa tcccttcttg
    12961 tacaaagtct cttcctaaaa tacgatctgc ttcttgtaac aactttaatg tcggagctac
    13021 ttgtttcaaa aaccagcgtt ctgaacgatc taatgagaat ttttctggct ttactgtaaa
    13081 atccaattct tccatcgaac gaaataattt tgcccatcgt ttatcaacaa tccgtgcatc
    13141 actttccagt ctatcccaaa caataagttt tgcattaatt aaaccaacgc caatctctcc
    13201 taaacgtttc ggtgaatcac ctaaaataaa tgcttctact gcttgtgttg ctttctcatt
    13261 ggctaaccgt aactcatatc gattataaat accatatttc gttaatgctt ctaattctga
    13321 aattttttct cggttcgcaa tttcaaaacg tttctcataa aaattaaaaa agagaggact
    13381 ctttcgcgat ccaaaataca aagataatcc ttcacttcga aaatagccat catctaaaaa
    13441 acaaccgcca atattctgga atctcttaaa gtccatttct aataaacctg ctttttgttt
    13501 ttctagtaat tcaaagagat cgaaatgttc ttccgtttcc aatccccaaa gttcatctaa
    13561 cgcaatatcg aatcggctac attgcacacg tgtataacca gcttccaact ttccttgatt
    13621 tttccaaatg gaatatagcc atgttcgcca atctgtttgt tgttcacgca tgacttcctc
    13681 atattcccgg caaccttgtc ctgaaaattg cacaagtaca ttttctgtta cagttcgttt
    13741 tgaatgccag aacacccaga tattcccacg aacagaaaga cagtcaaaaa gatacaagcc
    13801 cgttggacgt gtttcaaatt ctgtcagatc cattcgtaat acacgttgaa tcactgtttt
    13861 gtagtctgtc ccaaaaaagg aaagacgcaa ataatcaatc attgctcgaa gatctattcc
    13921 gcttttcata tacaatcgaa agcgttcctt gattcgttgt tcaaaatcct ctgggatttt
    13981 tcttttccct tgttccatct ttctaatgta atcttttgag cagcccatca attgcgcaaa
    14041 tcttgcttgt gatagcggct ttttatctcc acttatatta tgaattgcac ttaacttctt
    14101 tctgtagctt tttagctcaa tcggtgtcat tttctcgctc cttttataaa ataacaaaat
    14161 cgattttttt taacccttta gaaacaccga tatatcaact agtccccctc gttttttggg
    14221 aaaagtagta caactgctga ccttagataa tcacttgata caaaagggtt tataaaactt
    14281 ttttctatgt gttatgttat ttctgccccc catattatat atcggggggc tttacacaaa
    14341 gaggccgtgc gcaagcgcac gacctctttg tgagctgcac acgctgctag cgagtgcgca
    14401 ttataggccc atgcttcgca gccttaatgc gcaccgccag gcagagtgcg gctaccagac
    14461 tacgtctaga gccctcccga tagataataa ctacaaaatt aaatgatcta attttgttat
    14521 ttcactatat ttttcaggag aaaaaacgcg ataaattgac aaagttaaat cctcttctaa
    14581 atctactcga taatgataca atcgagtcaa tcgttctata tcttttttcg ttagcaatac
    14641 ttgattaata acttttcctg taaaaacacg ttctttgcgc aaaaagcggt aatcagttac
    14701 ttgttcaata cgattcaacc aatcataaaa cgttctggaa gaaagagaag gattaatcat
    14761 tcgtattgct gtagctactc tataataaca actctctgcc atttaacttc acctccttag
    14821 ttgattacaa aatatgcgca tcagaaaaac ttgttccttt tggatctttt gctaaataca
    14881 tttggcgctc tagttgttct tgtaacaact gattatcatc tcccatcgtt tgatcttgac
    14941 tatcctcttt tactgtaaaa tcatagacaa tcatttcagt aaatttctca aattctttga
    15001 taaagtgata atctttgtaa acaaatggcg caaaaaactc tacaggtaca tcaaatcctg
    15061 gaaaagcata tccacgacca ggttgatctt tgttgtaaaa tggcttattt ttaaaatctt
    15121 ctccaaaagt cattaaataa ccaatgggag acaatcgacc aacagaaata cggaatgtta
    15181 aattatcccg aacaccactt ggaaaatatt ctgcatctgg tcgttgcatt gctaaaataa
    15241 tgtacatccc tgcttgacga ccctttaata ctaatggctg aatcaattta tcgactctat
    15301 ttctcgcttc catatccaac gaaccataaa aagctgccca ttcatcaaaa aagataaaat
    15361 gcggtggtaa cccataataa gcataatttt tccctggttc atatttagga tctttcatgg
    15421 ttttcatata ggcataccgt tgattcattt cttttaatgc atttcgtaaa caaataatca
    15481 tttcttcatt tattttgtaa aacacatgtc ctttgaatac atcgacgtca cctaatgctg
    15541 ctaaatctgc cgcttttcca tcgcaaatat ctactgtccc aacacttaaa caagcttgaa
    15601 ttaaagaata caataaatag gttttcccac caccgattgc accagcaatc aacatatttg
    15661 gtgattgatc atatacccaa tccaaagttt tcattaaatg aatacgtcca ttttctgcta
    15721 tacattgatc aataggaatc cgaccagaat ctgaaaggaa tacataattg attcgggctt
    15781 tttctcgttc ttcacgaatt aaatcacgca aaaatacttc agataacaaa tgacccattt
    15841 ttaaaaatcg ttcgtgaaaa cgcccaccat ctaaaggaaa agaaaccact aattcttgtt
    15901 tcttttgata aaaataaact tttggtaatc tcattttttc ccttgtttgt ttctcactaa
    15961 cttgtttctt tgtataaaca aaccgattgt caattaacat tcgaacaagt aactgacgcc
    16021 aataaacaga ggcaaaatat ccttctttta gtcgcgacca atgcatcaac cctaaaatta
    16081 acccactaac cagcaacaaa tacaacacaa tacttattcc aggaataaga tacatttgcc
    16141 aattcgaaaa cgtacttaaa cgtggataaa atactttcca actaaaccaa cctaacagaa
    16201 tcaaaaatgg aaacaaaaag aaaccataat agcaaaacca taaataacga ttgaaatagc
    16261 gaattcgccg accacgatac ttgaaaaaat gagctttcaa ttgtcacatt cctcctttct
    16321 gtttttactt ttcctgtgct aaaaactttt gacaaaaagc aactaataca ggaatatcac
    16381 atttagtaaa aaaacataat cgaaaaagat ttaatcgatt tttataaggg tgtgcacaca
    16441 tattacatat tcgtactata cgaatccaaa tcacttcctg atgatcttgt ttccactggt
    16501 acggtaatga aaaacgaaca agatcacgta aaaacataca ctgcccaata accgcacaaa
    16561 tatatgcatt tgtttgtaca aaaatcgttg gattgtctcc aaaatactca atcaattgtt
    16621 ggatattttg ataacaagca caatctctca aagtatttga ttggttttat ctgaattcaa
    16681 aatctcccct tccttctatc ttagataaaa agaataggaa gactccttcc tattctttca
    16741 aaaaaacctt attttttctc tttttcctta gaatcaacac ttggttgttt ttgatctaaa
    16801 cctttttctt gtgattgttt catattagga ttatttgttg gagcttctac tttctctaat
    16861 cctttcgcat aaatcactaa atctgaatat tcacgattgc catcttgtgc tggaatatat
    16921 accacatgat attctggatc aattaatttc acaggatcac gtcgtttaaa accagtcaaa
    16981 tctacatctg ctggaaattt aactcgtcgt gcaatctttg gttcatctgg atttccaaaa
    17041 actaattgtg ctcgctgtga tcgaatttct cccgtataat cgcctgtttc attatcaaat
    17101 acacgatcag actcttctaa tgcgccaaag cgtacggctc ctaaacaagt ttcatctaat
    17161 gcttctgtaa tgtcataatt atttggaaat tttaatccca tcttacaaaa cctccccaat
    17221 ttttacacca gttaaaaaga caccaacatc ttgatagcca gttgttcgac gctctgtttg
    17281 tcccatatca tttgtttcac gactattcac tcgttcttta cgaaaaacaa aataagcttc
    17341 ttcaaatgaa atacgcacct ctttttctgg taatcgtaat cttttatcct cttcagctgt
    17401 gagataaaca aaaaaagcag gctgttcttc tcgcgaacac tctgcttgta attgaacata
    17461 tcgcccagac acaccattaa tttcctttgg tggattaggt tcccgtgaac ccaataaaaa
    17521 tacttgacct aaaaactctt tcttcatacg caatgctttt tctgccataa agtttttcct
    17581 cctcttaaaa accaaaataa attctaaata tttctacatt acaatcaaac aagcggttaa
    17641 aaatcctaac cgcttgttcc tcatgactaa tctttggctt tacgtacttt ataaagtgtt
    17701 atacctaaag caatgactaa tactactaat ccaagaacca taagtatact acttgtttgt
    17761 tcacctgttt ttggcaacat tggtacaaca gtagctggtt taacccgttg ttctggtttc
    17821 actggttgcg gtggttttgg cgcttccaca atttgaaccg tttgatcttt gtttgttaga
    17881 tctacattgt gtttcgccac ttcttcaaac gtttctttct catcttccgt tttcgcttgg
    17941 agccattccg caaaactcaa gccttttcct gcaagattgc gaccgtccac taagaattca
    18001 aataagaatt ccatcatttc tttatcggcc gcaaagactt tttccgcatg agtgccttca
    18061 aatggtgtgc cgtctggtgt taagtagcct tgaacaatta agcgatagat ttgaccaatc
    18121 gcaagaccgt ctactttgac ccattcaagc acagtttgat ttggcaaggc caagatctct
    18181 tttccgccat ttttattcgt cgctttcgtc gtaatggttg gattactgat tttgatggtt
    18241 tggtcattgt cggtaatatc tgcgtgagaa gccaataata cgccttctct aaacagatct
    18301 tcaaagacca cgacttcttt gcctcgcaac gcacgtgcgt ctagaataaa gtctagattg
    18361 actgtaccat ccgttgtttc tgccacaaac gttaaggtac ttgtgacttc ttttccatct
    18421 actagcactg gtttttccgt tactttgtcc attagaacac cgttgactgt gtatgttttt
    18481 ccaacgacca aatcgtggta tttcacagcg tctgtaatgg tgactttcgt taatggatta
    18541 acctctttca agccagtggg tttatgtgta gccgttgtac gtacacttgg gttagttact
    18601 cgtaccgttt gccctttgtc gttgatgtct ttatggtttg ctagttcttc atcatttcta
    18661 aaaagacttt caaacacgac gatttcacga ccatttatac ctgtcaaatc aaaggtaaat
    18721 ggtacgatca ccgtttcatt ttctgctttg gcaataaagc caacactcgt ttctaccgta
    18781 tttccatgca caagcaaggg ttcaccggtt gctttatcca ttaagatccc tttgaccaag
    18841 tatgcttttc ctggaagaat ctcggtaatt tcaacggtat cgtttaaggt aaacaatcct
    18901 gttgggtctg cgactgtcaa tccgtcttca aagacagccg tggtatgaac ggttggatta
    18961 ttcactttca cactttgggc ttcatcgttg atatccgcat gtgctgcaac ttcaatgcca
    19021 tcacggaaaa gtttttcaaa aacaacgatc gtttgacctt tcagaacact agcattaacc
    19081 acaaattcaa ggtcttttgt gccattgact ttgtctgcaa taaaggccaa ggtacttgtg
    19141 atttctttgc catcttgtaa aattggcgca ttcgttgcct tgttcatcaa tactcctgaa
    19201 atggtgtatt cttttccgac aaacaagttg gtataagaaa cactgtcttt aatgacgatc
    19261 gtttctaatg gatcaaccac tttttcacca tcggctttat ttgacgcttg tgtttctagt
    19321 tttggtggaa tcaagtaatt acgtacagaa accactaacg tttccccatc atctggcgca
    19381 ttttgtttga atgcaaccgt gaatgcttgt ttatctgtat ttggttggaa actttctaat
    19441 ggggcatttt ccaagaagta atagtcatcg gcaactagac ctttcactaa aatttccccg
    19501 ttttgatcag tggtatattc accaattgct tgatttttac cgttatacaa ggtaaagttc
    19561 acacctgcga gcggcgtttt cttcttgttc cattcttcat acttgaataa ttttacactt
    19621 ttccgtgctt gacggttcgc aaaatggatc actttgatcg gttctgtatc attggtaatc
    19681 gagaacacta atgggttttg attcaaaacg taatttacgg gggctttgat ttcttccaaa
    19741 cgatagcgat cttttccata agtaagtgta ccgttagtta ggaaaaagcc tttttcattg
    19801 gttttaaaga tatccgttgt ttctgtatca tttggaatct ccattgtgac ataacgattg
    19861 gctaaagtat caaaaatctt gaattctgta ttagcaagtg gaatcacttt tcctgtttct
    19921 tgatccactt tcaccacttt aagacgttgt tcaattactt tatcttctaa aacataatgg
    19981 aaagtttgcc cattttcaga aattgtgact tcaagtggtt caaatagttt atacccttct
    20041 tttcctttgg tttcagaaag aatgtacgta tcaaagacaa cggtattttt accaaaagta
    20101 gcataaccat cattgtctgt cacaacggtt ttcaccactt tgccagttgt ttttgacgtc
    20161 aaagtgattt caatgccagc taattttggt ttgttatctg gattcgttga atcgactaaa
    20221 tctttattag caactttcac taaatcaatg gttcctttga tcacttgatc ttttgcggtc
    20281 gcattgacag tttttccagg ttcaacaaca atttcaattg cttcatcaga aagcacataa
    20341 ccttttggcg ctttgatttc ttttaaaaca tatttgccag gatcaagatc tttaatttct
    20401 gcctgtactt tgccgttaat ttttttcaag gtaatttctt taatcttttt cccatctgct
    20461 tgatacaaac catagaccgc attttctaaa gaagctgcac cttgtgcttt cacaccagtt
    20521 tccgcatctt ctttaaacac acgaatgttt ccttgtttta gaatttgtaa attaataaca
    20581 gacgcattag gttctgttgg atgtaacaca ccaattgttt gagctcctgg acgacgatag
    20641 gctaatggcg caccttgaac tttaatttta ttcaattgaa tttttgcttt atccggtgcc
    20701 tcttttgtga ctgtaatcca tacttcattg ccgtgaatag atacattgac accttttggt
    20761 ttttgaatgt ctttcacata tttcaaaaaa tcatttttat tctttacttt caacgtttcg
    20821 ccaattttta ccgtatgtgt ttgattatta aaagttgctt tttcaccaaa gttattaatc
    20881 aaacgttgaa tttctgcttt tcgacgatca taatcaacac ggatattttt gattgggatt
    20941 tttgtgccca aagcttccca aatcataaat tgtgccgcaa tgtattgttc atcggatttg
    21001 tctttatttg tgacatatcc taaataagag atcaagcttg tttttttccg tacatttgaa
    21061 tttttcaaat agtttttcaa agcagtagct gtataaactt gaccttctgc ggctgagaca
    21121 ccaggctcaa tacaaaacgc cacttgtcca ttaatctcaa aacgaaccat ataatttaaa
    21181 gtgaagttta gattgttcgg actagaagta acaccaatat tttcgcccca atcaattttc
    21241 cgaccaccta aaacagctgc attggttgca cggcgaacac gaaccgaacg attggttggt
    21301 actgttacta acagtgtagg tgtacctgtt tttggaatgt tctgctcttt tttcttttga
    21361 ttttcaatac tagccgtaga ttctttgttt ggtttcgttg gttgattttg actagctgcg
    21421 gttgaagaga ttgttttttc aacgttttcc tcattttttc cttctttgtc agcagtagcc
    21481 gctggttgtt ccactgtttc ttctattcgt ttcttttctt ccgcttgttt ctcagaagac
    21541 tctggctgca cagaaggcgt tgcttcggca gctttttctc cttctagagt ggacgtttct
    21601 gaagcagggg ttgtaattga ggcagtttct tcaactggtt gtgaagtacc aggactattc
    21661 cctgggtgtt gtgtttcccc aatcgtctga tcgagagtcg ttttggaatt gaccgttggc
    21721 gcttctgtca caggggcctg ttcaacagtg gcttcaccag ctgggacccc ggcttctgct
    21781 ggttggactt ctgtcacttc agaagcgact tgggcaatgg cttccatcgt aggttgtata
    21841 gccgtcgttt cttctgcatg tgcttgagca gttcccacca agaaacagaa ttgagaaaaa
    21901 gccaccaatg cgagtgcttt caaccaattc attttcgttt ttgcatacac aagaattcct
    21961 ccttttcctg agaattttat ataataaaac aaaacgccgt taccaaaagt gataacgacg
    22021 tgaactgtct cttctatttt tatctggcgc tcttatctga acacatttca tcaatgattt
    22081 taccctccca ctataattac agaacaaaaa aagaacctgc ttcgtggaaa tcaactgtcc
    22141 atttcccaat tggaatacca gcttttttgg caacttcacc atcaccaaat ctcatgccat
    22201 accaattact accttcatcc ttaatttctt tccaaccata tgacatagct tcatctaatg
    22261 aatcaaaaat catgacacta ttgccaattg ttacaggatc tggcttaaga tcttctggaa
    22321 gagaaatatc aatcatacca taatgatccg attcctcagt attattgtca ttactagcta
    22381 ctggtttggt tgttctactg gcttattttg atacaacaag tactcttatt cttcttttac
    22441 ttctccgttt tcaatttccc attcaaacca ctccgcaaag tcttgcttaa aaccatctac
    22501 tcgataccta ttttgtaaat aggcatcttc tgacgtcgta ggatctaaat aggtaggttt
    22561 attctctgga cgagcataga cacctaccca tgtttcgtaa tctccttcaa aataactatc
    22621 taccaaatag cctttttttc taagaatctc ccgaacttga tttttaatta ttgatacttg
    22681 ttcaaaacca tcactcattc tatcacctca aataactcta gattctcata aatattcccg
    22741 acaatttcaa tcctatcaat gatctctaac aaatcgctac ttatgcttcc ccattcgtat
    22801 aagaatttcc cttccgcaaa ataaattttc cataaacttc ttcatgatcg tcccaaccaa
    22861 tatcactctc gaaaaattcc acgttgttct tatcttttag ctcagtaaat gccataagtt
    22921 caacatgttt tccaacctcg aaacaatcac accacccatc aaccgttaca cgtgtgcctt
    22981 caatacctaa gaaactttgt cctcgtgtat taaacactaa aactttccgc attttttgct
    23041 cattaattaa tcacgcgcga aatctaggaa tcacaactta ttcgcttctt ctttataaat
    23101 aaaaaaactc caataatcgg aaattactat cttttcctca ttcctttttt aaatcttcat
    23161 acttcttctt atctttgtcc taacctaata acacaaattt tgaagaaaaa ctcagtaaaa
    23221 cctgattctt tttttatctt cacaacaacc aactttttct tcagatcctt ttactagaac
    23281 ttgtatacaa aaattgttga atgctgatat aatttgcggc tacttgaaaa aacgatagat
    23341 gtaaaaagat aaatagaatc attttttatt ctatatcagt ctatgattca ttgcttatca
    23401 atcacccaac ccattcgatg ccgttttttt tagagaaatt taaaaaagac cgtacgaatc
    23461 tcgcattatc cgattacttt tccagaaaag atccccaatt ctgtataaat aaccctatct
    23521 tcttgttggt ttcattttct catataagaa ttcagtcaat ctacccacta tgaccattac
    23581 tacgtccaac acaactgaca tattaaccac gtaagtattc cctcacagat ctccgaatct
    23641 ccatggtatg gattcccatc ctgacgctgc agaggacaac atatttcatg tggctctgta
    23701 cccttttgat ttggaaacac tacaaatcaa tactcatcta taacttatca agcgaacttt
    23761 cgctaggctt ctagtgatta ctaaattgtt ggataacata catagcccat tttatctagc
    23821 tgttattcat aacagttagt tcttgtatta cttcttccgc ttgtgttgcc attgagcttg
    23881 tcaaaaagtt acttagaaaa aagctgccag taaagcaact ttcaacaaac cctttttgtt
    23941 tcttatcata tcaagaaacc tccttttata aattttttct ataataaaaa acgtcgataa
    24001 cttaataagt atcgacgtaa actattttta tatcccgtaa atcaaaaatt ctaaagaaag
    24061 atttcctata taagcatatt ggataattcg attagtattg ggtatatctc taccattctc
    24121 atacctatct atggaccatt ttgaaattga aggagaaaaa agctttccaa actcctccct
    24181 ttctagataa taatctttcc taattttcct catccttaat cccatactaa aagaatttat
    24241 ctttttaaaa tcgctaactc tttcatcttt tggaagtata ttcttataat cttctccgta
    24301 cataaggtaa ggaacagtga ccttaccaag ataggcaata ttcattagtt tttcaatatc
    24361 tggaatatta ataccttttt cccatctact tataacattt ttgcctgtat agatggtaaa
    24421 gtttttacca aatttttcaa gcgtctcttc ccggttcttt cggacttctc gtattctttt
    24481 tcccataaca attttatcgt cttttgataa actaaatatc tcactatcac gattaatctc
    24541 cttcaaaatt tctttttcat tacttaatag aaaaatatcg attttttttg catttctttt
    24601 gacggatttc tctttctatt taacacataa ctgagtaata cttcactaat tcctatatta
    24661 tcagctaact ctttgttaag taacttattc atatctctgt atgcaattag tttaccgatg
    24721 agaatctcaa tcttatcttc ctcatctttt attagagttt ctatattcag aatcttgctt
    24781 agagtattat ctacgtcttt agggattttt cttgaccctt tttcatattt aactaaagta
    24841 ctttgtgaaa tacctgctaa atcagctaac tttttttgag atagtttttt ctcttttctt
    24901 agagatttaa gaacccttcc acttgaatta tcaaccatga aaaaagcacc tcctatgaga
    24961 tgctttcatc atatactgac agctaaaaat cttcaagaaa aatattccaa ttttgttaat
    25021 tttagtatcg ccgtgataac caaatgacag tttaaaatct tttatacaga taatcctcag
    25081 tgttgcatct actcaaaaag gtttatttaa taggtttttc gattgattat aagttatttc
    25141 acactatctt gctcatccat tttcagaacg gccatgaagg cttcttgcgg aacttcgacg
    25201 gagccgattt gtttcatccg cttcttccct tctttttgtt tctctaagag tttacgttta
    25261 cgagaaacat ctccaccata acatttagcc aagacgtttt tacgcaaggc tttgatgtct
    25321 gaacgggcaa cgattttttg tccaatagct gcttggattg gcacctcaaa ttgttggcgt
    25381 gggattagtt ttttcagttt ttcaacgatt gcttttccac gttcataggc aaagtctcgg
    25441 tgaacgataa agcttaatgc atcgactttt tctccattca gcaagatgtc catcttcact
    25501 aacttgcttt tttgataacc agacatttca taatccaatg aggcatagcc tttcgtgctt
    25561 gatttcaatt tgtcgaaaaa gtcaaagaca atttcagaaa gcggaatatt atagacgaca
    25621 tttactcggt aatcatctaa ataatccatc gtaatgaatt ctccccgttt tcgttgagaa
    25681 agttccatta ctgcaccgac aaaatcattt gggaccatga tctgtgcttt gacgaatggt
    25741 tcttctacat cctgaatcgt tacaggttca ggaaaatctg ccgggttatc tactgtagca
    25801 gtcgtaccat ccgttttatt aacatggtaa ataacggatg gtgcagtcgt gatcaattct
    25861 agattgaatt cccgttccaa gcgttcttgg acgacatcca tatgaagaag tccgaggaat
    25921 ccacagcgga acccaaaacc taatgcttga gaggtttctg gttcgaattg taaagcagca
    25981 tcattcaatt gcagtttttc taaagcttcc cgcaaatcgt tgtaacgaga agtatcaatt
    26041 ggataaagac cacaataaac catcggattc atttttcggt aacctggtaa agcttcagcg
    26101 gcaggattgt ctgctaacgt tactgtatcc cctactcgtg tatcttggac agttttgata
    26161 cttgctgtga tataaccgac atcaccgacc atcaaaaagt ctctagcaac tgcttttggc
    26221 gagaagaccc cgacctctgt aacatcaaat gttttgccat tactcatcag tttgattttg
    26281 tctccgggtt tgaccatacc gtccgtgatc cggacattta gaacaacacc gcggtaacta
    26341 tcataaatag agtcaaagat caaagctttt agcggtgcat ctaagtctcc acttggtgca
    26401 ggaacatatt ccacgatctg ctcgagaata tcttcaatcc caatccctga tttggcactg
    26461 gctaatacag cttcactagc atcgattccg attacgtctt cgatttcttg acggacacgt
    26521 tcgggatctg ctgcgggtag gtcgatttta ttgatgactg gcaggatctc caaatcattg
    26581 tctaacgcta aataaacatt ggccaatgtt tgtgcttcga ttccttgtgc tgcatcgacc
    26641 actaaaacgg caccctcaca ggcagcaagg cttcgagaga cttcataggt gaagtcgacg
    26701 tgccctgggg tgtcaatcaa gtgaaagata tatgtttctc catttttcgc tgtgtaattg
    26761 agttctacag catttaattt aatcgtgatt ccacgttcac gttccaaatc catcgagtct
    26821 aacagctgat cttgcatttc gcgagacgtc acagtgtgcg tcatttctaa aatacgatcg
    26881 gccaatgtag atttcccatg gtcgatatga gcgatgatcg aaaaattacg aatctgctct
    26941 tggcgttctt tcattttctt tatatccata gcgtctcctc ttttcatata atcagcactt
    27001 tccattatac caacgaaaag cagtaattta aagagttttt aattagtcag ctatagaagt
    27061 tttttttgta gaattttgct atactgttat caagctaata aatggagatg aaaaaatgga
    27121 tcaaaaagat tttagagaaa atcttgaatt aaaaccggtc actcaagaac atatcgatca
    27181 attcaatgaa ttgctttcct atgtgttcca agtgactgaa gcagacatcg aggaaagcgg
    27241 gtttgagaat aaacgagctt ttatccgttc caaaaaaccg attttggaag tatcgaaagt
    27301 gttcggatgg ttccacgaaa ataaactgat ctctcaaatc gctatctatc cttgtgaagt
    27361 caatattcac ggtgcacgtt ataaaatggg tggtgtgact ggagttggaa cttatccaga
    27421 atatgctaac catggattga tgcaagatct aattatcgtt gcattggata atatgcgcaa
    27481 gaataagcaa tggatctctt atttgtatcc ttacagtatc ccctactatc ggcgtaaagg
    27541 atgggaaatc atgtctgata aactttcctt taaaatcaga gatacacagc tacctaaaca
    27601 aatcccagta agtggtatgg ttgagcgatt accagttgat catccagacg tattttccgt
    27661 ttatgacaaa tttgcccaac aaaaccatgg tgctttattc cgcagtgatt ttcattggga
    27721 agagtattgg cgtttcgaaa atgaagatga gcgcacagcc gctgtctact acgatagcaa
    27781 ccatgaacca agaggtgttc tgttctactg ggtagctgaa gaaatatttc acgtaaaaga
    27841 aatgttctat cttgatcaag aagctcgaaa tggcttatgg aactttattt ccgcacattt
    27901 ttccatgatc tactgggtac atggagatat ttataaaaat gagcctttag catttttgat
    27961 tgaagatagt cagatcaaag agcagatcga accatatttt atggctcgga tcgttgatgt
    28021 aaaagaattc cttcaacggt ttccgtttgt tggaacagct gatgcttttc atttcatcat
    28081 tgaagatcct gtcgctcctt ggaataatgg tgtcttcgct ttaacatggg atgaacaggg
    28141 acaagtccgt gtcttaaatg aacctatagg aaaatccgtt cgactggata tccaaacatt
    28201 gacttgcttg atgatgaatt atcgccgtgc ttcttatttg gcaagaatcg aacggttaga
    28261 aacagatgaa gaaacactga agtcactaga gcgtatcatt ccaaacatgg aagcttactt
    28321 tagcgattat ttttgataaa aaggatgatg tttagatgaa aatttatttt gccgcaccaa
    28381 tgtttgctaa aagtgatttg ttatataacc aacagttagt taaagaaatc agagagctct
    28441 cttctgagct ttcgatctat cttcctcaag aaaatgaagc aatcaatgac aaaactgctt
    28501 atgcagacag tcgaatgatt gctcttgctg acacagaaaa agtgttggct agtgatttaa
    28561 tgattgcttt actagatggg ctgacgatcg atgccggggt agcatcggaa atcggtgtgg
    28621 cttatgcgaa aggcatccca gtgatcggct tatatactga tactcgacag caaggcggaa
    28681 cccacccgaa aaaaatcgct gcactacaag aaacagctga aaatcaattt cactacctga
    28741 acttatatac aataggtttg atcaaattga acggaaaagt ggtttcttca gaaattgagt
    28801 tgctttcaga agtaaaaaga tttttagatg gagggacttt cagtgattaa agaaatcaaa
    28861 aaggtgcaaa tagctttact tgcttttggt gtattcgttg tctgctataa tttttatgaa
    28921 tttatcacac aaaaatattc gacttctcaa ggaatcacat ttatcgtgga atctttatta
    28981 gggattgcat tgattttcat gccacaagtc attttgaaag ttttcaaact taaaatacca
    29041 gcagcaatcg ttttgtttta ctggtttttc ttgttcatct ctgtcttttt aggcacaggg
    29101 atgcatttga tcagtatcat ctctttttgg gacaaaatct tacatgcggt gagtccaatg
    29161 gtcctgacag ctcttggcta tggattgatt ggttacttga tgaaagatgc tgagatatca
    29221 aaaaccagtc cttggttgtt cctcttattc ggctttgcgt ttgctggact ttgcggcgtg
    29281 ttttgggagt tctgggaatt cttatgcgac cagttcttag gcatgaatct tcaacgtttt
    29341 gctgcttctg atggcacact gtttgtcgga cgagcagcat tgatggatac gatgggcgac
    29401 ttgctgacaa atacgatagg tgctgcctta atgggattgt ttgcatggtc tcaaagtaaa
    29461 aaagatgagc gttactttga aagttataaa ttagaaaaag tgaaaaatac ctaataaaag
    29521 aggttgtgac agaagcgggc agcttcaaga aatttcggct tatttttcga agaaactact
    29581 tctggaacac tgtttatttg aaaaaaggag gtctgacact agttgtgtca gacccctttt
    29641 ttcttttata gtctcaatgg aagattagac gatttcttct agctcttctg ttttgaaatg
    29701 aagttgtcca tcaagtaaag taatcgttac ttttgattta ggcataacat gtcctgcaac
    29761 gatttcttta gctagtggtg tttctacttc cttagtgata aatcgtttca atggtcttgc
    29821 accatatgct ggttcataag cattttcagc aatccacgtt ttggcttcat cactgattgt
    29881 cagcaggatt tcttgatgtt ccagacgttg tgccaattgc gccaccattt tgtcaacgat
    29941 tcctttcacg ttgtctaggc tcaatggtgt aaataaaatc gtgtcgtcga ttcggttcaa
    30001 gaattctggt ttgaagtttc cacgtaatag cgtgttgact tgttcagcca ctgcttctgg
    30061 aatcgttcca tcagcagtca cgccttccag cagtaactgt gaaccgatat tgctggtcat
    30121 gatcagaacc gtatttttga aatctactac tcgccctttt gaatcagtca agcgtccgtc
    30181 atctaggact tgtaacaaga tattgaagac atcgggatgc gctttttcga tttcatccaa
    30241 caagacgatt gtataaggat ttcgtctgac agcttccgtt aattgaccac cttcttcgta
    30301 acctacatag cctggaggag ctccgactag acgagacaca gcatgttttt ccatatattc
    30361 actcatatcg atccgtacca tatgatcttc tgagtcaaac aagttctcag ctaacgcttt
    30421 cgctaattcg gtttttccga caccagttgg tcctaggaaa aggaaagaac ctagaggacg
    30481 atttgggtct tgcaatccag cccgtgaacg aattaccgcg tcactgaccg catcaactgc
    30541 ttcatcttga ccaatcactc gtttatgaag tgtttcattc aatttaatta gtttttctcg
    30601 ttcaccttct acaagtttcg ttacggggat acccgttaat ctgccaacga cttgagcaat
    30661 ctcattttcg gtaacggact cttgaaccat cttaatctcg ctgtcttttg ctttagcttc
    30721 taattccttc aattcttttt ctaattgagg tatcgtacca tgacgcaata cagcagcgcg
    30781 ttctaaatca tagttatttt ctgcatcttc taattcgtgt tttgctttat cgatttcagc
    30841 acgttttgct gaaacagaat tgacttcttc tttttctgtt tcccactgca tcttcatcgc
    30901 atttgcttct tcacgaagtt cagctaattc ttcttgcaaa tttttcaagc gttttttact
    30961 tgcatcatct gattcttttt tcagtgcagc ttcttcgatt tccaactgca tcaaacgtct
    31021 agtaacttga tctaattccg ttggcataga gttcatttct acccgaatcg ttgcactggc
    31081 ttcatcgatc aaatcgattg ctttgtccgg taagaaccga tcagtaatat aacggtcaga
    31141 taaagtagct gctgctacta atgcgttgtc atggatgtta acaccatggt ggatctcaaa
    31201 acgttctttt aatccacgca gaatactgat cgtatcttcc actgttggtt ctttgactaa
    31261 taccttttga aagcgacgtt ccaacgcttt gtctttttcc atatattgac ggtactcatc
    31321 taaagttgtg gcaccgatca aatgtaattc accgcgagca agcattggtt tcaacagatt
    31381 tccggcatcc atacttcctt ctgtttttcc tgcccctacg atattgtgga tttcatcgat
    31441 gaacaagagg atacgtccat cacttttttt gacttctttc aagactgctt ttagtctttc
    31501 ttcaaattca ccacggaatt ttgctccagc gatcaatgca cccatatcta atgaaaaaat
    31561 cgttttatct tttaaatttt caggcacatc tttgcgtacg atacgttgag caagcccttc
    31621 aacgattgcc gttttcccta caccgggttc tcctatcaga acaggattat tttttgtttt
    31681 tcgtgaaaga atacggatga catcacggat ttcttcatca cgaccgatga ttggatccat
    31741 ttttccgctt ttgacttgct gtactaaatc aacgccgtat ttttctaatg ctttgtattg
    31801 ttcttcttgg ttttgtgagg tcactctttc tcctcctctc atctcttcga tgtttttacg
    31861 caattctttt tcagataatc cgtttttatt caaataaaca gttaacggat aattttttaa
    31921 tttcatcaac gctaaaataa cgacttccgt tgaaagaaac tcgtcgccga agctttcacg
    31981 tatttggtct gcttcattca ataatcgaaa gaaattctgg ctgaggtttt gtccatattg
    32041 gacgtttcca ccagaaacaa ctggatactc atccagtaga cgatcaattt catgttcaaa
    32101 agattcgaca tccaatccgg catccgtata gaaatttcga ccaaaatgat tagattgtaa
    32161 aaagattttc catacatggg caatgtcgat atcttgatgt tttcgagtga ccgcaatctg
    32221 ttgagcttca gcaatcgctt cttgaagcgt cgtagtcatt ttctcaatat tcatacaggt
    32281 ataccctcct aattaaaggt aagatcactt atttctttga tctttacaaa aactattata
    32341 tcgctttggt caattttggt caaaatatac acatagtttt ttgacttttt ttctcgactt
    32401 atataaatct taaaaaaaca aaattgattg tatgtatata aaaaaagagc caaacaaacg
    32461 taaaattatc ttatccataa cgtttttctt tgatttgata aaaagggtag atcttataaa
    32521 agacctaccc ttgattttct ttattctctt tttttacttc tattatttct tctttgatca
    32581 ctcttcgccg ttcagcaatc acatacacaa atccatagag aaatggcaag ataaaggaac
    32641 tgaaacgata cagtaaaagc gaagaaactg cgtcaactgt tccaacaact ggtctgaata
    32701 ataacaaata gacaaattca aaggaaccaa taccagcagg cgtaggaata acccctgaaa
    32761 gcactactgc aaagctgata aacgaaatgg tcagtagaaa atctatttta ggatggtttt
    32821 caatcagaat caaataagga agtacatacc aaaatacaag ttttaacata ttccatagat
    32881 aaatccgcaa tagcgcagtc ctgtcttgga taatggtttg gaccgtttcc cttaaagagt
    32941 agatctgctg attgcatgta tccacccatt tccgcaaacg ctcactttta aataacttat
    33001 tcgataagct gacaaaaaat atttgaacat tcaagctgac ggacatcgcc aataaaaagg
    33061 caacaatcac gatcgtcaaa aggataccag cgatgataaa ccagatcatt gatggaccat
    33121 tagaataaaa aagagagaat tggatgacca agccagctaa tgcataagtg atcactgcca
    33181 gtttgtacat aatcatatgg agtgctgtca cccctgctcc ctgggatagc ttcattcctt
    33241 ttttgttata aaaataaatc tccgagatca gcgttcctgt accaaacgaa acgattcggt
    33301 aaaacgcaat atagctagag gtcaagaacc catctttcgt tgtaaaattc ggcgcgaaag
    33361 gttcagcaat ttctttgatt gagcgccctt cagcgatttg gtaaactgtc cctaatataa
    33421 cagccgctgc caaaacaaca acactggtag acagcaattc ggcaaagata tctgcaaatg
    33481 attgattgat taaataataa ataatggcaa aaatcacaat cagcaaaagc agattcagaa
    33541 tgattttagt tttcgttcct ttagacgaca tctcctgctc cttaatggaa aaaataaatg
    33601 agaatcaaat caacaagtac acctgcagcg aatcctatag taaacttctt tttccgagtt
    33661 ttatgatgga aaacttttcg agagatcaat cctcccaaac caccgcctaa caggctgaca
    33721 aatatcaaat tattctcagg aacacgccag ccgctagttc tagcctgatg tttatcatac
    33781 cccatcagac aaaataagta aaaattcaca atgaaaaggt aaaaccacaa aggattgtgt
    33841 aacaatacat ccattactct tcttccaact ttctgttaac tactatttct aagctttttc
    33901 ttctttcttt ggtgaataaa ctgttaatcc ttttgataca acagataatt caactggaag
    33961 atcatctgtc gtgtctccat ccgttcgagt gcccacagat ttttcctgcg cttggatttt
    34021 catgcgtttg gctgtgaaat aagtaatgcc ggggatcgta tagaatttgc ctctcatgat
    34081 tctaggcaaa taaaaaataa atcggaaaat atctaaggaa ggtacgatcg ttacatgaaa
    34141 ttttccatca tctagggtcg ctgagtcgtc aaaatttgta aaaccgccca cggagttaga
    34201 catcgtgact gtcaacagtt gggattttcc tttccaatgc tcctcgtctg tttctaaatc
    34261 caaatgatat tttttcttct tcagcaataa cttaaaaaac ttcttgataa agatccacga
    34321 gccgtaacgc tgtttttctt cttgactgat ccaaactgct gtatctgcta agattccgat
    34381 tgtcagtgta gagataatga cttcatcatt gacttgacca tagtctattt tccgtacatt
    34441 gccttccagg atcacgtcag cagcttcttc ttttttgagg ggaatctcaa gaacacgtgc
    34501 aaggttgttg acagttccac ctggaagtaa tccgacatgg tattgagata atgttttttg
    34561 gaacgcttgg acgatatgat gaatcgtgcc gtctccacct ataattacta aagtatcagc
    34621 ttctatggct tctgctttct tgactaattc cgcatcatca atcgatgggc cagtttcagc
    34681 tagttctaca tataattctg gctgcttttc ttttacataa tcttgaaacc agcgtgctaa
    34741 tttctcaccc tcatctttcc ctgaactttt gttataaaaa actagcagct tttccattct
    34801 ttacctccta tttactcttc aaaaaagagg gcgggacaac caattccatt gtcctgccct
    34861 ttagccttag acagatgaca ttccatcatt gggacgaacg ccttgtcata agctgttctt
    34921 tttcgatttt aactctttgt gttcaattct gcaattttta caatcacgtc tgttgctttt
    34981 tccattactt gtaaagaaac aaattcgaat cgtccgtgca tattttctcc tccggcaaat
    35041 agatttggcg tcggaatacc catgtaagag atttttgaac catctgttcc tccacgaact
    35101 ggttcgatca ctggagagat accaagctcg atcattgctt gtttcgctaa ttccacgatg
    35161 ctcatatctt tttcaatgat ttctttcatg ttgtaatatt gatcatacat atctactttg
    35221 attcgttctt gatcaaatcg ctgattcaac tcttcttgga tatgtgtgat ttgtgctttg
    35281 cgtgcttcga atttttcacg atcatgatcg cgaatgatat aactcataga agcttcttca
    35341 ggattcccat ccaatgccat caaatggaag aaaccttcgt atccttccgt tttttctgga
    35401 acttcatcgg ctggaagctg attatgaaaa tcgattgcta actgtagagc attgatcatt
    35461 gtatcttttg ccgtccctgg atgtacattt ttcccttgga tcgtaatatt tgcttgagca
    35521 gcactaaacg tttcatactg caactcacct aaaggaccac catcgactgt ataagcaaaa
    35581 tctacatcga attgctcaac atcgaattta tccgctccga caccgatttc ttcatcagga
    35641 ccaaaagcca ctttgatggt accgtgaggg atagaaggat tattgatcaa gatctccatt
    35701 gcagtcatga tttccgcaat ccctgatttg tcatctgctc ccagtaatgt actgccatct
    35761 gttgtgatca atgtgtggcc ttcataattt ttcaaatttg gaaaatcttt tgtattcaat
    35821 gtatagcgtt cttccgcatc tagtacgatc gtagactcgc catcatagtt ttcaatgatt
    35881 tgtggattga ctcctactgc attaaaatct gctgtatcca tgtgggaaat aaatccaatg
    35941 gaacgcactt catgatcaac attgcttggt aatgttccca taacgaatcc gttatcttca
    36001 ttatagaaaa catcagctaa gcctaactct tctaattctt ttttcaattc ttgcgcaaaa
    36061 gcaacttgtg tctgagtcga tggagtcgtt ttgcttgttg catcggagcg tgtttctgtt
    36121 ttgacatatc gtaaaaaacg tggcaaaaga ttttcgtaca tagtattctc tccttttaat
    36181 taaattgaaa tgggtttgta tttgtttcag aaacgaaaaa gttaatatcc caattttctt
    36241 cttgtttcca agattcaaat ttctcgataa atcgttgctt gcataacgat tcaatataat
    36301 gaccgggatc aattgcaatc aacccagcgc tttgcatatc atgagccgta tgataataga
    36361 tatctcccgt gatatagaca tctgctcttt gggcgattgc ttgcggataa aatttttcac
    36421 cgcttccacc gcaaatcgca atgcgcttca cagaggattt tgcatttttt ggctggacta
    36481 ttctcagtcc atctagttgg aacgcttctt ttacttgttc tacaaatgcc tctatagaaa
    36541 tttcttgcgg caattcacct actcgtccta acccaaacat ttcgactggt tcatctatag
    36601 cgaaaagatc ataagcaggt tcctcataag gatgagctga ccgcatggct tgaatcactt
    36661 gtttttcgat agtttccggc aaaataacct caacttttgc ctcttgaact tgttctgtct
    36721 tccctacttt cccaatcgct ggttgtgcac cagcttccgg agtaaatcga ccgtgtccaa
    36781 tcgaggtaaa acttgtaccg gtataatcac cttgtgtacc agcaccagct gcagctaata
    36841 cttcacgcat tttctgtgca tgatccacag gtacataaac ggccagtttt ttataatgaa
    36901 tctcatgagt ctttactaaa taactctcca cttcaatacc gagaagttca caaaaccagt
    36961 cattcagtcc atcccaaata atatccatgt tcgtgtgtgc tgcatagacg gcaatatcat
    37021 gtttcaacaa atctgcatac attttttctt gtggctgatc agtaaccagc cgttttacag
    37081 gacgaaagat tggaggatgt ttagcaatca gcaaatcgat ttttttcttg attgcttctt
    37141 cgaccacttc tggacgaacg tctaatgtca tcattactcg ttggattggc ttatccaatg
    37201 ttccgatatg cagtccgact gggtctcctt cttcagctag ccactgagga caatagcttt
    37261 caaacttttg gatgaaagtg cgtccgctga ttgtcatggc tttaaaacct cctcaatcat
    37321 ttggatttcc tgctcgattt gtttttgacg tgcctcttga ttagctgctt gtttgagctg
    37381 ttcacggata gcttttcttt gttgaagctc tcttgtccat tttgatttga agatctcact
    37441 tttctcttca agcagaaatg gcccgaacat cagctccatt tcattgtaag taactggttt
    37501 ttgttctttt tcggctacaa tgatttcata tattttttta ttttcttcaa gtattttttc
    37561 tgattggatt ttatactgat tttcttgcag ccaagatcgt aatgtacgct ctccgatgtt
    37621 tggctgaagg atcaaacgtt cttgtccgct taagcgttga ttcaacttgc cagcttctag
    37681 gatatctcgg atcagtgaac ctcccatacc cgcaatcgtg acagctgtaa taccatcttg
    37741 tggctggata gcatctagac catttgcaag gcgtacggtg atacggtctt ctaatccatc
    37801 ttttttgact tgcttacgtg ctgattcata aggaccttga acgacttcac cagcaactgc
    37861 atattcgatt tttcctctta atatcaatgc aacgggtaag taagcgtgat ctgatccaat
    37921 gtctgctaaa cgagcggatt cagggacaaa gtcacccact gcggctaagc gctgagataa
    37981 atctaaatgg ttcatagctt cactccttct agataattat agcattttcc aaataaaata
    38041 aggagactag gaataaaaaa attcccatat ctccttaaaa aatatacctt ttcgttctat
    38101 aaaacaacat tttcttattt ttgaaaaaat gttattggtt atttgttcaa tgaaacatca
    38161 ataatcgaat ctccaggaac ttgcggtggg atagacatcg tcatgatcgg catctcaact
    38221 aatgtaaatt ggactttgtc accgattttc aattcattta tgtccacgct tattttttca
    38281 gcagtagcat ttaagatcac tccgtcattg ctgaatgacg ttcccatatt cgctggatct
    38341 tctttggcca tgacatcgac gagtttcaac tgaagcgttt cttcttctgc ttctacttga
    38401 gtgatacttt caatcgttcc gacaaatatg ctttgtctct ccgttttcgt actgcttgtt
    38461 tttactgttt gacccgcatc aatcgaatca acagaacctg tgttgctatc tgcgctaccg
    38521 cagcctgcta aaagaaaaaa tagaccaact acagatatac tagaaaaaat agccctcata
    38581 aaaaatcctc cgtttaagtt tctgcattac ttgttgcggt gattgtctcc ggtcatttct
    38641 gtccatataa tgattcgctg acctgctttt actactaggt caaatgtcag ttggacttgt
    38701 caactgttca accattgcgt cggttgcgac cagtcatttc gatttctttt gtcaaataac
    38761 gtatgcgttc cattatccgt ttcgctttta gcggctcttc atctccacgt tgagtgacgg
    38821 acaaatgctg ttccaattca tccattgtaa aattcgatga gaagaaggtt ggtaactgtt
    38881 cctgcatccg atattgaagg ataacaccga atacttcgtc tcgtatccaa ctactcatcg
    38941 catctgcacc aatgtcatcg atcatcaaaa taggtgaacg tttcactgca tctaactttt
    39001 ctgccacttg atcttttccg attgcctgtt tcatctcaac ggcaaatgtc ggaaaatgga
    39061 ctagcgtcgt ggtgaatcca gcaatcgcta attctctcgc tgttgctcct aataaatatg
    39121 tttttccaat accaaaactt cctactagat acagtccttt atgataccct tttggatcag
    39181 cagtatattt ctctacaaaa tctagggctt ctaagctcgc atatcctcgt ccatctgtac
    39241 gttcaaagtt ttcaaaagaa gccgattgaa tatcttttgg catatccatt gcttttactc
    39301 ggcttctgat ttcttcttgt ctttgccgtg ctaacaatgc ttccgttggt acataagtga
    39361 catccacata atgaaaattc aacgtcagct tcggctcata gccgggagcg atcatcgttg
    39421 gatcgttttt ttgatattta cgcttttctt gcacgaattc atacaattta gcgtaacttt
    39481 tttcaatatc cgcttgtgtc aattcttctt ggtgtgcgct tagaaaagct tgaacatccg
    39541 catcatttaa gacttcagcg atcatttcag catacttttc ttgataattg cgtttgctga
    39601 tgatcctctt caattctttg cctacgtctt ccactagata tcaccctcct tttttgccag
    39661 gtattcttta aaacggcggt caatctctgc ttttttctcg ctgctgatct gtttttcatc
    39721 ttttggctgg ttgacccaat caggaagctt ttcccgtcga acatttcgct tgccagtttg
    39781 ataatttgtt tgcttctttt gttttgattg ttttgcttga ttacttattt gccgaatgtg
    39841 ctggatcgct tcttctgctg tggcgatttt cttttgtgcc cattcatttg ccactgtatt
    39901 tacataactg gcatttaagc tagcattatt cttgatcact agtacataat tcagcaaaac
    39961 attgatcacg ctactggaaa gacctgattg agaaacaaga tcttgcagta accagttttc
    40021 ttgtttagaa acatatccgc ctttttctga cttgattgct tccagatatt tcatcggtgg
    40081 aatgctctgt gcttctttta tcagctgaag cgctgcgttt gtcaaatgtt cgtttgacga
    40141 gggttcatta ctgttttcag aaaccgcaac tgtttctttt gtttgcttca ccggacgtct
    40201 ctgcattgcc aaaatggctt tttgcagttc tttttcagat actttttgtt cactaaagtc
    40261 ataagattga ctgatcagtt cgccaagtgt caattcatcg aaaccgtaaa gctgatgata
    40321 catgtcgact tgtttgatga ttggatcatc aagacttgca atatgttttt ttgccagcca
    40381 atcattcaaa aactgccaat cgaatgtcga atctgcttca agtaatgcta ctgattcaga
    40441 ttcttcaaaa gatttattca gcgattcgat gttttcttga ttagcagcgt acactgcttc
    40501 attgaagcga taaacatcta aaaatttttt tgtgattttt tgatatccct ctgctgtatg
    40561 ccttttaggt tcaaagaatt gggtaagctt ctggaatcgt cgttcaccaa ctttttccag
    40621 aagaagaaaa gccagaacag agtctttgaa aaatgcttta ggaatgagtg gttctaacag
    40681 ctcataaata aaacgaggtc ccagatcact atcttcttta aaataaacat ccagtaagcc
    40741 gattccttct agcttttttc ttgcatcata aaattgcggg ataccgcagc ctagactact
    40801 aagcagatct gcatgcatcc cttcaggacc tttgccagat tcttgagaga tttctgataa
    40861 cagggtgaaa tagatactca aggcttctcc gccgattagt ggctgataaa gatacagcaa
    40921 ggcgttcttg ctgtcttctc tcagcggagc cgcttttctt ccttggtaaa tattttttgg
    40981 ttgaagctct ttccaagcat tttccaagga aatgcctcct ttttgtttct taattgtttt
    41041 ctttattatt ctctttatta ttttcttttg ctttatccac gatatcttgc aactctttta
    41101 agaaaacaga catatctttg aactggcgat aaacacttgc aaaacgaata taagcgattt
    41161 cgtctaaatc aactaaatct tccatcacat attcaccaat tagattcgtc gatacttcgt
    41221 tttctccgag actgcgtacg cggttttcga catggttgac gatttgttcc atttgttcca
    41281 tagctacagg tcttttttct gctgaacgga tgagtcctct taaaatttta tcgcgattga
    41341 actcttcgcg ttccccgttt ttcttgatta ctaataacgg agcagcttca attcgttcaa
    41401 atgtagtgaa tcggaacgaa caattctcac attcgcgacg gcgacggatc gcacgtccat
    41461 catctgcttg acggctgtcg attactcgtg aattattatg ttgacatctg ggacatctca
    41521 ttttgtctca cctcttgcat cgcattttat ataaaggatg tgtctttctt tacaactagt
    41581 tgtattataa cacgaaacaa atcacaaacg cgactgtcct acccaaaaaa cacggatata
    41641 agaacaattt tttctacttt catgcgtaat tgtgtcccct gtccttacta aatttcaatt
    41701 ttccaatact gatcaccttg attactgaga aataacttac acaaaagctg atttagtcat
    41761 ctagcacaaa aaacttgaag cagatgaaca ccttctcatc tgcttcaagt cacatctcga
    41821 ttttttacta ttttataact tctcttcgat tcctgtatag tgtttcattc gctgttcctc
    41881 gattttttgc tcatcgggaa tgtggtcact ttctgggagc ggtgttttac tacgatcagg
    41941 tgtcggattt tttcgttctt gagtcaaatc ttgccgcttc gcttcttctt ctgcctgctg
    42001 gtcttctatt tcatcgattc ttgcttggta ctgcttttct ttttcttctg ctgtttctga
    42061 ttgcgtacga ccttcatcga ttagattttc tttattgagc atctcgccga ttttttcttt
    42121 tgcgttacct acgactttat ccttttgatt ttttaatgat cccatcgcta tccctcactt
    42181 tctatatact caggatagca aaattgacac agatactcaa agaaagcgat tagcaaaaag
    42241 ccaattttcg atttgtccca caagtatttc ttttgttcca ttgttgtcga acaaaatatc
    42301 cgcacgctgc tttttttctt cgattggaag ttgactacta attctctgct gcgcttgttc
    42361 tagcgtcagt tgattacgtt tcatcaaacg ctgaagctga gtttctggcg tagtataaac
    42421 gacagctact gcatccatat agtggtcata atgaccttcg tagagcaatg ggatatctac
    42481 aattactaaa ggatgattgg aacttagaga ggcgatttga tcttttatcg cttttcggat
    42541 aaacggatct aatagctgat caagtttttg ccgtttttcc tttgatggaa aaacaatcat
    42601 cccaagtttc cctcgatcta gttgcccatc aggctgcaat acctcttggc caaacactga
    42661 tacaattttc ctcagagcaa gcgttccagg ctcaacagtt tctctggcaa tcacatcgcc
    42721 atcgatcaaa ggaaatccat gagactgaaa gactttggct gccgtacttt taccggtagc
    42781 tattccaccc gtcaaaccta aaacaaatcc tactttgcga tttttagcct ttgacaattc
    42841 ggacaatagt gcgtccccct ttgtgccact tttgttttca cgatcggcgt tccacaacgt
    42901 atacatggtt tgccagtttg ctgatagaca tgcagtgcta cttgaaatcc gcctgattct
    42961 cctaatgcat tcaaataact gcgaatcgtg gtacctccgg cttctattgc acgatccaaa
    43021 acatcaatga tcgatagacg taattgttct acctctttgg ggcgcagtgt gttcgccggt
    43081 tgttctggat ggatcttcgc ttcccataat gcttcatcta cataaatatt acctagacca
    43141 gtcactagac gttggtctag cagcaatggc ttgattgctt tgtgggactt tttcagtcca
    43201 tttgcgaaat ctgctaataa gaagctatct ggcaaaggct caggtcccaa tttcatgatt
    43261 cctcggtaag ttgcacttgc tcctttttcg acgattgtca tacgaccaaa ttttcgtaca
    43321 tcgttgtagc gaagttggct tccatcctca aaaaagaaaa gtacatgcgt atgtttatcg
    43381 attggtgtat tttctttcgt aaattgatat tttccttcca ttcgcaagtg tgagatcagt
    43441 tcacaatgat cgagatgaaa aattaaaaac ttccctctac gtccaatgga ttggatggtt
    43501 tcgccaacaa gagaagcctc aaagatgggt gtctctggtt gctcgatgat cttgggccac
    43561 aagacctgaa ctttttgaat cgtttttcct actactaacc gttccaatcc tttacgaacg
    43621 gtttctactt ctggtaattc tggcattttt tcttttcacc ttcattatat tgctctgtct
    43681 taaacaatgt ttattttgct tcgtaccacg tttttcccca actgctgtca gtgatcagtg
    43741 gtacatgaag agaaacagct tgttccataa cttccttcac caatttatct aatgattcaa
    43801 gttctttttc cggtacctcg aagacaagtt catcatgaac ttgtaacagc atcgtagctt
    43861 gaagacctgt ttcttttaat cgtttatcca attcgatcat cgcgattttc aaaatatcag
    43921 cagcacttcc ttggattggt gtattgatag ctgttcgttc cgcaaaagaa cggatattgt
    43981 aatttcttga attgatgtct ggcagatatc gacggcggtg atacagcgtt tcgacgtagc
    44041 cttggtcttt tgcttcgcgt acgattcttt ccatatattc tttgacacct gggtattttt
    44101 caaagtacgt atcgatgtat tgctgtgctg cttttcggct gattcctaga ttttgtgaaa
    44161 gtccgtaatc agagatgcca tacacgatac caaaattcac tgcttttgcc tgcctacgca
    44221 tatttggtgt gacatcctct gccttttcga ttccaaatac acgcatcgct gtgctggcat
    44281 ggatatcttg accttctagg aaggcagctt tcaaatgttc atcatttgag atatgcgcaa
    44341 gtacgcgtaa ctcgatttgt gaatagtcag aagaataaat cagccagtct ttttctctag
    44401 gaacaaatgc ctgacgtatc tttctccctt catctaatcg aatcgggatg ttctgcaaat
    44461 taggatcaac agagcttaat cgtcctgttt gggtcaatgt ctggacataa cgggtatgca
    44521 cttttccatc tgattggatc actttcagca gtccctcgac ataggtagat tgaatttttg
    44581 caatttgacg ataggtgagg atatcctcta cgattggtgc ttgttcacga agctgttcca
    44641 gtacatctac ggctgtggaa tatccagttt ttgttttttt gataacgggt aatcccattt
    44701 tttcaaataa gatcactcct aattgctttg gtgaattcaa attgaattct tcgccggctt
    44761 cttcgtagat tttttgttcg atctctctta agcgagcaga gaactcgccc ttcatttctt
    44821 gtaagcgagt cgcatctact ttgatcccgc ttatttccat ctctgccaaa atgatagaca
    44881 atggcagttc cattttccgg aaaagatctt cttggttttt ttctgccagt tcttggctca
    44941 attttggtgt caacgcattg attgcagcta cttttcgcgc taaatgagca aaaaagagtt
    45001 cctcttcttc tggtaaacct tttttcgccc ctttgccgta tacagcttca tctgattgga
    45061 tatcgttgta tccgtaatga gcagctattc cttcgatatc tgtgcttttt tcattcgtat
    45121 cgagaaggta tgcacctagc aagacatcaa aatcgattcc tttcgttttt ccagtatagc
    45181 ggtttaaagc aacataagtt cgtttcgcat catatacttt tttcagccga gtcggatcag
    45241 tgatccaaga atggaacgct cggttttcga atacagatag atcgttcgtg acataaattt
    45301 tcttttctgt cccccacgct actccgacga tttcctctgt atgataattg tcgcccatca
    45361 tttccacgta caacgccata tccgtagtaa acatttcctc tttgaattca tgaacgactt
    45421 caaacaaaat atcatccatt tttacttctt cttctacgat attcaattta gaaaggaact
    45481 gattgaaatc catttcttta taaaacggaa cgagtttatc gagatttttc ccttcatact
    45541 tcaatgagtc gatagagaca tccacaggcg ctgaagtatt gatggtcgcc aattttttcg
    45601 ataagaaagc ctgctctttg tcatttatca gattttcttt cattttgctt tgcttcattt
    45661 catcgatatg ttcataaatt ccttcgactg agccatattg tttcaatagc ttgatagctg
    45721 ttttttcacc gattttagtc acaccgggga tattgtcaga ggcatctccg gctagtccct
    45781 tcatgtcaat aatctgtttt ggcgtcaacc catcatattt ttccgctaca tgttctggtg
    45841 tatagctttc gatatcgctg acacctttta cagtgatatc cacttttact tctttactag
    45901 ctaattgagt caaatcccga tcacctgaaa gcacgacgac atcaaattga tctttgtcta
    45961 ctttttctgc caaagtaccg ataatatcat ctgcttcata attgggcaat tcgtaatact
    46021 gtacccctag tcctgtcaac aaatcacgta tatatggcat ttgttctttg aattcgccag
    46081 gtgttttgga tcttccacct ttgtactccg catacatttc tgtccgaaaa gtcgtttttc
    46141 ctgcatcgaa cgcaaccaga acatgagtcg gttgctcttt ttgcataaca ttttcaaaca
    46201 tcgtattgaa tgcatagatt gcatttgtat gaagaccgtt tctatttttg aaacgttcaa
    46261 gagaattgtg aagcgcaaag aatgcgcgaa aagccacact gttcccatca acgagtaata
    46321 atttattttt tgtcatcttc ttaactcctt actgtaaagt tcttattcat tctaacaaag
    46381 aatcctgcgg aatgcgaaaa gaaaaagcgg gaccatacca aataattcgg tatagcccca
    46441 actgatgaat ttttttcttt tttagttgtt ccgtccaaag attctcagaa acgccaagaa
    46501 caagttgata aagtccaaat acagcttcaa tgccatgaat acagcaattc cactatgtgc
    46561 tgttccgttt gtctggacat agatattacg gatgctttgg ttatcataag ctgtaatccc
    46621 tgcaaagatg acgactaaca gtagggagat gaacagctca actacgctac ttctcaaaat
    46681 gaagatgttc aagactagtg caatgatcac tccgattaaa gctgcctgtc ctgctcttcc
    46741 aaccccgcta agatctcttt ttgttgttac tccgacaaca gacatcccca tgaacatcaa
    46801 agtagctgtc aagaaagctt gcgttactgt tccaatatcg tagtatgcaa gagtaaccgc
    46861 taatattatg ccattcaaaa gagagtaggc aataaatcca ccgattgcta agctcggatt
    46921 cttttgtgct tttacgccta gaaccattac taaaatcagt tcaacgatcc acagacccac
    46981 aaagcctagt ggaaaattat tgataaaagt tgctaattgc aaaggaaata cccaaagagc
    47041 caagtaagaa gtgactgcac taagggcaag tcccatacct aaaaatccat aaacttttgc
    47101 ataaaaacga ttgatacctg acattgatgt acttggatta ttcattcaat tcaccttcac
    47161 tttcgattgc tatttccaat tcaggaactt gtggcggttg gaccataacg acagcatcca
    47221 acacacgatc ttcgttcgta ttctccgctt ctacagaaat cgtcaccaca tccttcatct
    47281 tatctacttt tatgacctca aactgaaaag tcagtgtttc ataatggaaa accggttcaa
    47341 taaaattgac agaaaaattg acaacatgtg atcctggtcc aggaagatgc tttgaaattg
    47401 cacttgtgat gatccccatc agcatcactg atggaacgat tggctgttga tattctgttt
    47461 tttgtgcaaa gtcatgttgg atatacaaag gatttgcatc attcgtcaat cccaaataca
    47521 gcaaaagctg gttatcttca atagattctg ttaaggataa cgagtcgcct tcctcgattt
    47581 catttattgt ttttcctagt tttctcggct tacctatgtt caagatagaa accctccagt
    47641 ttatttttaa tcatacatta ttgtaccaaa agtagcgtaa aaggcaagtt ctgctccctt
    47701 gatttaacag attcctatag tttacagagg tcgtgaaaaa gctgtcctgc atcaaataat
    47761 aagaacagcc aagaaaaaac agcttctcat gtttttgatt tgattgggct tattaccgca
    47821 gatgcaagct tttgaacacc gtttatcata agttgcaaaa aagggggctt ggcaagattt
    47881 ttgccactcc ccctctttct atttttataa ggacactgca gtaattgtat tacagtacat
    47941 ttttcgtcgt gctgcttaaa agtttttcat acgcttgttc aaatttttga acatctccag
    48001 cacccatgaa gattactact gcatcatgat aatctaacaa tggagataca ttattttctt
    48061 tgatgacttc tccacctttt ttgattttcg ctccaaggtc ttcaatctta acgtttcctt
    48121 gttcttcccg agccgaacca aaaatatcgc ataagtaaac tttatcagct aaatcaagtg
    48181 cttctgcaaa ttcatccatc aatgcaatcg tacgcgtaaa cgtatggggt tggaaaactg
    48241 caattatttc tttatctgga tatttttggc gtgcgccatc aatcgttgct ttgatctctg
    48301 cagggtgatg cgcataatca tcgactacgg tcatgtctgc tacgatcttt tcactgaatc
    48361 gacgttttac tcctgggaaa gtcagcattt cttctgcaac ttctttcaga tccagttttt
    48421 cgaaataagc tactgcaata acacctaaag catttaagat attgtgttta ccgaatgctg
    48481 gtacagtgaa gtgcccaaca aattcatccc catgatagac atcaaaagca gaaccagaag
    48541 ttgtgcgttc gatgtttcgt gcttggatat catcgttttc tgtgacacca taataataaa
    48601 tcggcacatt tgcttttaat ttgcgtaaat aagcatcgtc accatacgca aaaatagctt
    48661 ttttcacttg tccagccatt gtttggaaag ctgtaaatac atcgtcgata cttgtataat
    48721 aatccggatg atcaaaatcg atattggtca tgattgcata gtctggtgaa taggcaagga
    48781 aatgacggcg atattcacag gcctcaaatg aaaagaattc cgcttgcgga tcaccatgtc
    48841 ctgttccatc tccaattaga taacttgttg gacggacacc actcaataca tgagagagca
    48901 gtccagtcgt acttgttttt ccgtgagagc ctgttacagc gatactcgta taattttgga
    48961 taaaatgacc aataaagtcg tgatagcgaa tcacttccaa acctaattct tttgctcgct
    49021 ggatttcttc atgtgaatca ggaaatgcat ttcctgcaat gatcgtcatc cctggtttta
    49081 cattatctgc gttaaatggc agaatcgtga tatttgcttt ttctaaatct ctttgtgtaa
    49141 agaaatattt ttcaatgtct gatccttgga catttagacc ttgttcatgt aaaacaagag
    49201 ccaaggagct cattcctgaa cctttgatac caacaaaatg atacaattta ttttgatttt
    49261 ccatttgaat cctcttctct tatctagcgt ccattctttt agctattaca ttcaacacgc
    49321 tcctcattat cttataaacg attgaaaatt tcaaccaaat ttatttggaa aggaggattc
    49381 gcttgctaaa atggtcaagt acgagaaaaa tccgttttta ttgactcttt aattctggtg
    49441 taagaagacc aaacttcctc tgatatatca atgtgaggaa taaaatcttt tcctttcgga
    49501 ccatagccgt tctcatcgtc atggagacgt tccatttctc ctaatactag cgcatggaaa
    49561 tattccttag gccattgatg attcagttct tttgctgtaa agatgatctc ttcagaaaag
    49621 tgaaggattt ccggcacaaa tggcacagca ttcgcgaaaa tagtttgttc attttgccat
    49681 actttctcaa aagttgctcg agtcctgcgt tgcagtgtcg gatcattcat caataagatt
    49741 ttttctggta cagctcctaa ggaatgaaga atctccagtg aaaaagtggc gttctctcca
    49801 gaatttgtag acttcgattc tatcagaaaa gccttatcgg gcaaatcata gacttctttt
    49861 agatactgac gacagatttc actttcactc atgccatctt caaaatgaaa tccttgcttt
    49921 tcaaaattct cataaaggat tctagtcgca tgccccaccc ctccgaccaa aaacaattga
    49981 tctacttggc catttaaata catttgtgct gctttatcag ctaagatagg aagacagttt
    50041 ccagctaaaa taagtgtagg tgccgtcagt tcctcaaaat ttccctcttc aggtgcggct
    50101 aaaaaattta atagctgatt ccagtctttt agattcctct gctcactttt ttccaaaggc
    50161 cttctatcct ttcatctgat tttttgtgct tcattaaagt cttctctttt tgtctcaatc
    50221 catttgattt ctccatgaca tcttccacaa cgatatttgc gtacatcgat tcttctcttg
    50281 cggcagatcg tggcagaaca atcttggcat cgataatact ccatctttat ctgtttcttc
    50341 gtcaaggctg gtgcatagcg caaccctcct gtttttttca aaagatactt gaaatccata
    50401 tctttatgct gataccctct tccagctaga tgaagatgat aatggcaaag ttcatgtttg
    50461 atgattcctg caatcgtctc ttcgtctgac tcttcaaata gcttcgcatt aaaatctaaa
    50521 tgatgatcgt ttaaatgata gcgcccgcca gttgtacgta agcgtggatt gaacattgct
    50581 tggtgcttga aaggaagctg gaagcttttt aatgatatct cttctactaa ctgctgcagt
    50641 tcttcttgtg tcaatttttg ttactcctca ttgttactct tcataagggg aaagcatcgt
    50701 caaactgatt ctgccttttt tcgtatcgac ttgttctatc caaactgtta cgatatctcc
    50761 tactgacacg acatctgtag gatgcttgac gaattttttg ctgagtttcg atatatggac
    50821 aagtccgtct tgcttcacac cgatatcaac aaatgcgccg aagtcaatga cattacggac
    50881 cgttccagtc aattccatcc ctggtttcaa gtcttccata ctcaatacat ctgtacgtaa
    50941 taagggcgct ggcatctcat cacgcatatc tcgtccaggt tgtgtcagcc cagctaaaat
    51001 atcggctaac gtttcttctc cgactgatag gctttcagcc agtttctcta cggataatcg
    51061 tgtcaatttt tctactgctt cttctgtacc aagctctttt tctgaaagtt ggactgaagt
    51121 taatatttct tttgcaatag aataactttc gggatggata cctgtgttgt ccaaaatgtt
    51181 tttcccacca gggactcgca agaatccgat tgcttgttca tatgcttttg gtcctaagcg
    51241 tggcactttt ttcaattgag ttcgtgcggt gaattctccg ttttcttcac gataaatgac
    51301 aatgttttgg gcagttgttt tattcaatcc tgatatatgc tgcaagagct gcgggcttgc
    51361 tgtattcaca tccacaccta cttggttaac cgctgtttcc acaacaaaat ccaattgttc
    51421 agccaaacgt ttttgagaaa cgtcatgttg gtactggcct actccgacag ctttaggatc
    51481 gattttgact aattcagcta aaggatcttg caggcgacgg gcaatagaaa cagcacttct
    51541 ttcttcaact tgtagatgcg gaaattcttt tcgtgcaatt tcgctagcgg agtaaacaga
    51601 agcgcctgct tcgttgacga tagcgtaata ggttttatgg tcggcggatt tcaactgctc
    51661 agccacaaaa agttcagatt cccgactggc tgtaccattt ccgatagcta ccatatctac
    51721 ttgatattta ttaattaatt ggataaaagc tggtccagca gcttctcttt ttgcttgcgc
    51781 tgcaggttta tgaggataaa taacttcgat tgctaaaact ttccctgtcg catctacgac
    51841 tgctagttta catccagtac gataagccgg atcaaatccc aggacaactt taccttttag
    51901 tggcggttgc aacaaaagat tacggaggtt ttcgccgaaa atagcgattg cttgctcatc
    51961 tgccttttca gtcaattcat tgcgcagttc tcgctcgatt gctggctgaa taaagcgttt
    52021 ataactatcc tgataggctt ctctgacgaa tgaagaagaa ggagaggcag gatttttgac
    52081 tatttggcga tccaagtaag ctaaaattgc tgcttcatct acttgaaaga atactttcag
    52141 aatatcttct ttttctccac gattagtagc taatattcga tgagaaacca ttttatggac
    52201 gggttctgca aagtcataat acatttcata tacgcctttt tcatctgctt gctcatcctt
    52261 gacattgctg acatacatac ctttgttata cgtataagac cgaatccaag tccgaaattt
    52321 tgcattatcg ctgacttgtt cagcaataat ctcatgagca ccgtgcaacg cttcttcggc
    52381 agaagagact tctttttctt tgtcaatata tttttctgct tcagattgca cctctccatc
    52441 tgtaagctgc atcagccact tcgcgagtgg ttcaagtcct ttttctttag caatcgttgc
    52501 ttttgtccgt cgtttttgtt tgtatggacg atacaaatct tccacttgtt gcattttcac
    52561 ggcttgggtg atttctgctt ctaactcggg agtcagtttc ccttgttcat cgattagccg
    52621 aatcacttca gttttacgtt tttctaagtt ttcaagatag gcataccgct cttcgatttc
    52681 acgaatctgg acttcatcaa gacttccagt catttctttt cggtaacgcg cgataaaagg
    52741 aacagtgttt ccttcattta acaaattaag tacagtagtc aactgcttag gacgatagtc
    52801 tgaaagttct ttttgtacaa gctgaatgat cgtctggttc aaattttccg tcattaaaat
    52861 gcctcttttc tgctaaaaat tgcttcttta ttctatcata ctttttatcc tgggttaaac
    52921 tactgaaaga aacaatattc tctataaaaa taaaaagagc cgcaacaaag tcacagctcc
    52981 ttcattcttt ccattagtca gattccatgc caaggtcctt cgttgttctg atattcaata
    53041 tcttgaagaa taccttcctt gtcaaaaaca accccatgca atgaccctcc gaatacagcc
    53101 ccaccgtcca ttccgatttt atgatcctga atccagaggt ctgttgtctg catatctcca
    53161 tggagcattg gtgtaatcgt atgtccaaat acaattgtct tacctgtatt gtttttccct
    53221 tcatgaaatg gttcccttat ccaaataaag tctcttagac ttgtcttacg ccagtctttc
    53281 tttgtcaaat ccacaccggc atggacaaaa agataatttt cccattcata atacaagggg
    53341 cgctggacta aaaatttaat cagttctgaa tatctcgatt ggatcatcat cgagatttca
    53401 gttggggagt attcctccga tgcccctggg tgaatcaggc tttcaaacgt ttcttttcca
    53461 ccatttctca tataaggcgc ataccagtcc tccggtgatt gcagaaattg caaaaaatat
    53521 tcttcatggt ttccccggag gtaaatggct cctgtttctt cgactaattg ctttcctaat
    53581 aaaaaacatt cttttgtacg aggacctcga tcattgagat cccctaataa aacaagctga
    53641 tggatctttg gatcataata gtgaataagt ttttcaaaaa gctcatgttt gccatggaca
    53701 tccccaatag cataaatcaa tgatttcatg tggatcacca tcctcaacgc tagtatagcg
    53761 catattggaa atggtcgaca atcaaaacta ttgttttcta tttaattaaa gctgtaaacc
    53821 gcagttctgc ttcttctgga gagattccat ggtcttgaac atagcgattg cgcggatgtt
    53881 tcgcacactc tatgctacat gctcctaaat gtttttcttc attttcaatg gaagcaagaa
    53941 tctgacgatt gcactcagga ttgctacaat tgatatatcg ttcacatggt gttccgtcaa
    54001 accagtcgcg accgacgatt tgtttatcta cttggttgat atcgacagcg atccgttcat
    54061 caaacacata catcttgccg tcccataatt ctccttggac ttcaggatcc ttaccataag
    54121 tagcaatacc gccatgcagc tgtccgacat ctttgaatcc ttcacggaca agccaaccag
    54181 aaaacttctc gcaacgaatg ccaccagtac agtaggtcac tacccgtttt tccataaatt
    54241 cttctttgtg ctcgcggatc cagtctggaa gctctcgaaa actgcgtata tctgggcgta
    54301 cagcccctcg aaaatgtccc aagtcatatt cataatcatt ccgtgcatca atcaccactg
    54361 tctgttcatc taaaatagct tctttaaatt cttgcggtga aagataggca cctgtcactt
    54421 ggtttggatc aatgtcctct tctagcttta aacttacaag ctctggacgg tatcttacaa
    54481 acatcttctt aaaggcatgt ccttcgcttg gatcgatctt gaacatcgta tcagcaaatc
    54541 gaggatcagc tttcattgct ttcatatagg cagtcgtgtt ttcaacggtt ccagacacag
    54601 taccatttat tccttcttca gctactagaa tacgaccttt aagatccaaa gatttgcaaa
    54661 aggctaaatg atttttggca aattcctctg gttcttcgat ttttgtataa ttgtaataaa
    54721 gcagtacttg ataatccatg tatttcttcc ttccttcttt tcgttcctaa acagtataaa
    54781 gaaggcgttc gcatttgacg atttatttgc ttgtgtataa ttacgcatgg aaagtttttt
    54841 gtttacataa tattattatt tgaattaaaa agacttcgct ttcctgtttc gcacagaaaa
    54901 acaaagtctt tatttctatt atttacctgt aaccgcaaag tagttgccat cgggatcagc
    54961 aaagttaaag acatattctt ctccaagctg taccatatca cctaatgtga ctgattgcgt
    55021 ctgcattttt ttatatagtc caaagatatc tttactgtaa aacatcaaag atggggcact
    55081 agtagctgtg ccaggtgaat gggactcaac aaatgctcga tcatataaca gaaatgaggt
    55141 ttgactgtcc tcaactccta cttcaattat aagcgtccca tccatttctt gacgatcttt
    55201 ttccgcaaag ccaactgctc tccaaaacgt tgcatttttt tctacatcgt ccacataaag
    55261 catgattttt atttcattcg taaacatc
//
LOCUS       DAZUJO010000009        55276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000009 DAZUJO010000000
VERSION     DAZUJO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.009"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..710
                     /locus_tag="ACG8OL_000600"
     CDS             <1..710
                     /locus_tag="ACG8OL_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFS4816610.1"
                     /translation="NQLERIIDNFILFHDKVFKIVLKTQSPLEVKKYLKQFRPKQGIY
                     FLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTL
                     DEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLS
                     LCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNF
                     TLGKAQKIKEKLKESTV"
     gene            complement(1413..1628)
                     /locus_tag="ACG8OL_000601"
     CDS             complement(1413..1628)
                     /locus_tag="ACG8OL_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS4816611.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1647..1883)
                     /locus_tag="ACG8OL_000602"
     CDS             complement(1647..1883)
                     /locus_tag="ACG8OL_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS4816612.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2141..4294
                     /locus_tag="ACG8OL_000603"
     CDS             2141..4294
                     /locus_tag="ACG8OL_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS4816613.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4263..5678
                     /locus_tag="ACG8OL_000604"
     CDS             4263..5678
                     /locus_tag="ACG8OL_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS4816614.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5711..6745
                     /gene="sppA"
                     /locus_tag="ACG8OL_000605"
     CDS             5711..6745
                     /gene="sppA"
                     /locus_tag="ACG8OL_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS4816615.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6747..7268
                     /locus_tag="ACG8OL_000606"
     CDS             6747..7268
                     /locus_tag="ACG8OL_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS4816616.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7265..7651
                     /locus_tag="ACG8OL_000607"
     CDS             7265..7651
                     /locus_tag="ACG8OL_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4816617.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8147..8219)
                     /locus_tag="ACG8OL_000608"
     tRNA            complement(8147..8219)
                     /locus_tag="ACG8OL_000608"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8184..8186),aa:Lys,seq:ctt)
     gene            8600..9169
                     /locus_tag="ACG8OL_000609"
     CDS             8600..9169
                     /locus_tag="ACG8OL_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS4816618.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9156..10016
                     /locus_tag="ACG8OL_000610"
     CDS             9156..10016
                     /locus_tag="ACG8OL_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4816619.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10206..10910
                     /gene="yycF"
                     /locus_tag="ACG8OL_000611"
     CDS             10206..10910
                     /gene="yycF"
                     /locus_tag="ACG8OL_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS4816620.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            10915..12750
                     /gene="walK"
                     /locus_tag="ACG8OL_000612"
     CDS             10915..12750
                     /gene="walK"
                     /locus_tag="ACG8OL_000612"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS4816621.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            12747..14063
                     /gene="yycH"
                     /locus_tag="ACG8OL_000613"
     CDS             12747..14063
                     /gene="yycH"
                     /locus_tag="ACG8OL_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS4816622.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14064..14933
                     /locus_tag="ACG8OL_000614"
     CDS             14064..14933
                     /locus_tag="ACG8OL_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS4816623.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            14988..15797
                     /locus_tag="ACG8OL_000615"
     CDS             14988..15797
                     /locus_tag="ACG8OL_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4816624.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(15900..16529)
                     /locus_tag="ACG8OL_000616"
     CDS             complement(15900..16529)
                     /locus_tag="ACG8OL_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4816625.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            16699..17991
                     /locus_tag="ACG8OL_000617"
     CDS             16699..17991
                     /locus_tag="ACG8OL_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4816626.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18067..18729)
                     /locus_tag="ACG8OL_000618"
     CDS             complement(18067..18729)
                     /locus_tag="ACG8OL_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4816627.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            18954..19238
                     /gene="groES"
                     /locus_tag="ACG8OL_000619"
     CDS             18954..19238
                     /gene="groES"
                     /locus_tag="ACG8OL_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS4816628.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19289..20914
                     /gene="groL"
                     /locus_tag="ACG8OL_000620"
     CDS             19289..20914
                     /gene="groL"
                     /locus_tag="ACG8OL_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS4816629.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(21003..22142)
                     /locus_tag="ACG8OL_000621"
     CDS             complement(21003..22142)
                     /locus_tag="ACG8OL_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4816630.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALENYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKMIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYGEGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLSNRAKELIESVIA
                     DNILAGRPTNPDQYIFISTNGTPLTLHSFNAVLHKVEEELELEKNLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKKAKQQVIDKLNRL"
     gene            complement(22253..22837)
                     /locus_tag="ACG8OL_000622"
     CDS             complement(22253..22837)
                     /locus_tag="ACG8OL_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS4816631.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(22964..23395)
                     /locus_tag="ACG8OL_000623"
     CDS             complement(22964..23395)
                     /locus_tag="ACG8OL_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4816632.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(23413..23733)
                     /locus_tag="ACG8OL_000624"
     CDS             complement(23413..23733)
                     /locus_tag="ACG8OL_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4816633.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            24027..24167
                     /locus_tag="ACG8OL_000625"
     CDS             24027..24167
                     /locus_tag="ACG8OL_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816634.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            24465..24722
                     /locus_tag="ACG8OL_000626"
     CDS             24465..24722
                     /locus_tag="ACG8OL_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFS4816635.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(24693..25001)
                     /locus_tag="ACG8OL_000627"
     CDS             complement(24693..25001)
                     /locus_tag="ACG8OL_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816636.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            25079..25825
                     /locus_tag="ACG8OL_000628"
     CDS             25079..25825
                     /locus_tag="ACG8OL_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS4816637.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            25840..26043
                     /locus_tag="ACG8OL_000629"
     CDS             25840..26043
                     /locus_tag="ACG8OL_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816638.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            26148..26447
                     /locus_tag="ACG8OL_000630"
     CDS             26148..26447
                     /locus_tag="ACG8OL_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816639.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            26447..26617
                     /locus_tag="ACG8OL_000631"
     CDS             26447..26617
                     /locus_tag="ACG8OL_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816640.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(26676..27155)
                     /locus_tag="ACG8OL_000632"
     CDS             complement(26676..27155)
                     /locus_tag="ACG8OL_000632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816641.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            27200..27907
                     /locus_tag="ACG8OL_000633"
     CDS             27200..27907
                     /locus_tag="ACG8OL_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS4816642.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            27900..28028
                     /locus_tag="ACG8OL_000634"
     CDS             27900..28028
                     /locus_tag="ACG8OL_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816643.1"
                     /translation="MNRAEALKIGVIIANRWWRHNKPNILSQQHIDKQKAWQQIKK"
     gene            28086..28427
                     /locus_tag="ACG8OL_000635"
     CDS             28086..28427
                     /locus_tag="ACG8OL_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816644.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            28420..29166
                     /locus_tag="ACG8OL_000636"
     CDS             28420..29166
                     /locus_tag="ACG8OL_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS4816645.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            29172..29858
                     /locus_tag="ACG8OL_000637"
     CDS             29172..29858
                     /locus_tag="ACG8OL_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS4816646.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            29865..30698
                     /locus_tag="ACG8OL_000638"
     CDS             29865..30698
                     /locus_tag="ACG8OL_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS4816647.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            30715..31566
                     /locus_tag="ACG8OL_000639"
     CDS             30715..31566
                     /locus_tag="ACG8OL_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4816648.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            31563..31922
                     /locus_tag="ACG8OL_000640"
     CDS             31563..31922
                     /locus_tag="ACG8OL_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS4816649.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            31937..32098
                     /locus_tag="ACG8OL_000641"
     CDS             31937..32098
                     /locus_tag="ACG8OL_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4816650.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            32095..32400
                     /locus_tag="ACG8OL_000642"
     CDS             32095..32400
                     /locus_tag="ACG8OL_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816651.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKTR
                     RRMPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKNMDIDSKGRQFRYVEEK"
     gene            32400..32717
                     /locus_tag="ACG8OL_000643"
     CDS             32400..32717
                     /locus_tag="ACG8OL_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS4816652.1"
                     /translation="MNLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            32714..33100
                     /locus_tag="ACG8OL_000644"
     CDS             32714..33100
                     /locus_tag="ACG8OL_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS4816653.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            33097..33432
                     /locus_tag="ACG8OL_000645"
     CDS             33097..33432
                     /locus_tag="ACG8OL_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816654.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            33432..33566
                     /locus_tag="ACG8OL_000646"
     CDS             33432..33566
                     /locus_tag="ACG8OL_000646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816655.1"
                     /translation="MKDWLEEASALVGCFINCAFISVIIALVGFVSLKVIILLWQLIF
                     "
     gene            33578..34147
                     /locus_tag="ACG8OL_000647"
     CDS             33578..34147
                     /locus_tag="ACG8OL_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS4816656.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            34144..34317
                     /locus_tag="ACG8OL_000648"
     CDS             34144..34317
                     /locus_tag="ACG8OL_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816657.1"
                     /translation="MSRQIMLHIPDGTKAMTITIVAAQKDGGLALKTKSIDTKQILEG
                     RDVEIEIDEEEAE"
     gene            34314..34619
                     /locus_tag="ACG8OL_000649"
     CDS             34314..34619
                     /locus_tag="ACG8OL_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816658.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            34643..34840
                     /locus_tag="ACG8OL_000650"
     CDS             34643..34840
                     /locus_tag="ACG8OL_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFS4816659.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            34837..35043
                     /locus_tag="ACG8OL_000651"
     CDS             34837..35043
                     /locus_tag="ACG8OL_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816660.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            35043..35339
                     /locus_tag="ACG8OL_000652"
     CDS             35043..35339
                     /locus_tag="ACG8OL_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816661.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            35415..35828
                     /locus_tag="ACG8OL_000653"
     CDS             35415..35828
                     /locus_tag="ACG8OL_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4816662.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            36512..36679
                     /locus_tag="ACG8OL_000654"
     CDS             36512..36679
                     /locus_tag="ACG8OL_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816663.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            36705..37049
                     /locus_tag="ACG8OL_000655"
     CDS             36705..37049
                     /locus_tag="ACG8OL_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS4816664.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            37054..37335
                     /locus_tag="ACG8OL_000656"
     CDS             37054..37335
                     /locus_tag="ACG8OL_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816665.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVDIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            37438..37752
                     /locus_tag="ACG8OL_000657"
     CDS             37438..37752
                     /locus_tag="ACG8OL_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS4816666.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            37730..39424
                     /locus_tag="ACG8OL_000658"
     CDS             37730..39424
                     /locus_tag="ACG8OL_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS4816667.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            39444..40622
                     /locus_tag="ACG8OL_000659"
     CDS             39444..40622
                     /locus_tag="ACG8OL_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4816668.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            40585..41271
                     /locus_tag="ACG8OL_000660"
     CDS             40585..41271
                     /locus_tag="ACG8OL_000660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS4816669.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            41271..42431
                     /locus_tag="ACG8OL_000661"
     CDS             41271..42431
                     /locus_tag="ACG8OL_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS4816670.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            42441..43316
                     /locus_tag="ACG8OL_000662"
     CDS             42441..43316
                     /locus_tag="ACG8OL_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816671.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            43313..43624
                     /locus_tag="ACG8OL_000663"
     CDS             43313..43624
                     /locus_tag="ACG8OL_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816672.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            43614..43967
                     /locus_tag="ACG8OL_000664"
     CDS             43614..43967
                     /locus_tag="ACG8OL_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS4816673.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            43957..44358
                     /locus_tag="ACG8OL_000665"
     CDS             43957..44358
                     /locus_tag="ACG8OL_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816674.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            44351..44755
                     /locus_tag="ACG8OL_000666"
     CDS             44351..44755
                     /locus_tag="ACG8OL_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816675.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            44767..45372
                     /locus_tag="ACG8OL_000667"
     CDS             44767..45372
                     /locus_tag="ACG8OL_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4816676.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            45392..45754
                     /locus_tag="ACG8OL_000668"
                     /pseudo
     CDS             45392..45754
                     /locus_tag="ACG8OL_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            45757..45939
                     /locus_tag="ACG8OL_000669"
     CDS             45757..45939
                     /locus_tag="ACG8OL_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816677.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            45957..49376
                     /locus_tag="ACG8OL_000670"
     CDS             45957..49376
                     /locus_tag="ACG8OL_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS4816678.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            49427..50164
                     /locus_tag="ACG8OL_000671"
     CDS             49427..50164
                     /locus_tag="ACG8OL_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4816679.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            50174..52465
                     /locus_tag="ACG8OL_000672"
     CDS             50174..52465
                     /locus_tag="ACG8OL_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4816680.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            52489..54570
                     /locus_tag="ACG8OL_000673"
     CDS             52489..54570
                     /locus_tag="ACG8OL_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816681.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYG
                     SDYTFAITYTI"
     gene            54587..54736
                     /locus_tag="ACG8OL_000674"
     CDS             54587..54736
                     /locus_tag="ACG8OL_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816682.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            54733..55179
                     /locus_tag="ACG8OL_000675"
     CDS             54733..55179
                     /locus_tag="ACG8OL_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816683.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            55181..>55276
                     /locus_tag="ACG8OL_000676"
     CDS             55181..>55276
                     /locus_tag="ACG8OL_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS4816684.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT        19323 a         8629 c        11326 g        15998 t
ORIGIN      
        1 taaatcagtt agaacgaatt attgataatt tcattctttt tcacgataaa gtatttaaga
       61 tagtattgaa aacacaaagt ccgttagaag ttaaaaaata cctcaaacaa ttccgaccaa
      121 agcaaggaat atatttctta gatattgatt taaatcatga agttaacggt atagaattag
      181 cagaagtaat cagaaaatat gatgttcaag caaaaatcat ttttacaact actcatgatg
      241 agatgttacc cgtaacaata aaaagaagag ttgaaacgtt aggatttgta acaaaagatc
      301 aaacactaga tgagtatcga aacgagattg ttgagttatt gttattagcg caagaaagga
      361 tagatgcaac aaaacaggtt acgaatcaag catttgtttt ttccataggt tctcaaacat
      421 ttacttttga tataaatgac atttattttg tagaagcttc aaaattacca catagattat
      481 cattgtgtac taaagatggg cagtacgaat tttatggaag aattagtgag cttgaaaaga
      541 aatatcctat gttaacaaga attagtcgag cgtgtttagt aaatatcttt aacgttaaag
      601 aaatagattt taaaaaacgt tcactatatt ttgattcaga gttggccaga aactttactt
      661 tagggaaagc acaaaaaatt aaggagaagt tgaaagaaag cactgtttag tggttttttc
      721 tttagggaga ttgcattttt ctaataaaat agattccttc actagtgcaa atttttctga
      781 atcctattaa agttgtacca atttttatag ctgttattag ttacgtgcat aacagcaata
      841 ttagttactg gaggaatatt tctatggtct ggaaccaaaa aagggccaaa tgaatagttc
      901 ttttcttgtt tttattaagt accataaagg ggaattaaaa ttaaatgtat ggtggaagat
      961 gcgaaagatt tggcaaattc tactaatttt tagaaagtgt ttttcatttt ttagttaaaa
     1021 aatttgtcct gtcaagtgca atcctatgat tgtacttgat aggatttgag ataattactt
     1081 tcctgttttt ataaatttaa cttgcaggaa caggctttaa tgcacatccg acaagtacat
     1141 taccaatacc tcctgtaacg gccgaaatag gtcctcctgc agcaccaact acagcgctgc
     1201 aagtcctttt gtaaaaatat ctctaccatt acatttccaa agaattttta tctgacttta
     1261 ataaaagata aaaatttaat ataattataa aaactagcac aagaagcttt agtaaaaagt
     1321 tctgtccatt ccaaactttt gaaaagataa agaacatacc tatgttcgta atcgacacaa
     1381 accaatcttg aggtttgata ttttttaatt gtttacgcac cggtccacat gctgaatcca
     1441 gcacctaata aagctccacc tacaccacaa gcagctattg cataaggtcc tccggctgaa
     1501 caaagtttta ctcctgctgt tgccccagaa aaagtagtgc ctaaaactcc cacggcactc
     1561 ccaccttgaa tatttttcaa ctcttctgaa gataatataa atgttgtttt tgtgttttta
     1621 atatgcatac tacttattct ccttttttat ttttttcgag catcccagcc agctgcagca
     1681 cctccagcga cagctccata taaccctcca acaacaccgc aagctggtcc gcaaattgct
     1741 gttcctaaac ctgcaccacc tactgcccct gtggtaagtc cacctacaac ttgccccaat
     1801 tttccccaag cattattgcc tccaccaaca gtttgtgcat ttccacaata cttagttcct
     1861 ttacattttg catgtttaaa cacgcttctt taaataatat tttttacggt gctactgtaa
     1921 catacgtatt tgattaacag taaaaatctt cctgaaactt atcaagttta ttcttgaata
     1981 gtaaaatttc caaaataaat ttgattcagg aatgaaaaag ttagtgttca agaactgttt
     2041 tcaaagtttt tgtggaatta tgagacaata ttttcactgt attaaagcta tattgagaat
     2101 ttttctataa aaatttttta gaaggagttg tgttagataa gtgataaaat catttccttt
     2161 tgtgcagcaa caagatgaaa aagattgtgg tgttgcttgc ctttcgatga ttttaaaata
     2221 ttttaaaact gatatcccga ttcataaatt acgggaccta tctgggactg atttagaggg
     2281 tacttcagca tttggattga aaaagacatt tgaaaaattg tcttttgatt gtatggctat
     2341 tcaagcagat agtgagatct ggaaagaaag agatcttatc ttaccattaa ttgcacatat
     2401 attggttgat aacagctata tgcactacgt tgtaatttat aaaacgaagg gaaacgtact
     2461 atggattgca gatcccgcta atggaaaaaa tagaaaaacg attgaagcgt ttcaaaaaga
     2521 atggacaggt attcttttat tacctatgcc tagagaaact tatattccta ttaaagaaaa
     2581 agtagctgga ttaagttctt ttttgcctat aatttggaaa caaaaaggat tagtattcca
     2641 catcgtttta gcatcgattt tcattacgtt ttttggcata ggaagctcat attattttca
     2701 aggtattctt gattttttca tccctaatca agcacgttca actttaaata taatttccat
     2761 tggcttgatc gctgtctatt tatttcgggt attgtttgaa tatagtcgta gttatcttct
     2821 tattatttta ggacagcgaa tgagtatggc aattatgctt cagtatttca aacatgtgtt
     2881 aactttacca atgaattttt ttgctacgag aaaatcaggg gaaattattt ctcgttttct
     2941 tgatgccagt aagattattg atgctttagc aagtgctacg ttatcaatat ttttagatat
     3001 tggtatggtg ttgcttgttg gggttacgtt agctattcag aatagtacgt tgtttttaat
     3061 cactctatgc tctttgcctt tttatgtagt tgcaatattc gctttcgtga aaagctatga
     3121 aaaatcgaac agggaagaaa tggcagccgg tgcagctgta aattctagta tcatagaaag
     3181 tcttaaagga atagaaacaa ttaaagcata tagcggtgaa gagaaagtat ataaccgtat
     3241 agaccatgaa tttgttaaat taatgaaaaa atcatttcgt acggttacgc ttgataatat
     3301 tcaacaagga attaaacatt tgattcaatt aattagcagt gcattaattt tatggctagg
     3361 ttcttatttt gttatggata atagaattag tctaggtcaa cttatcacat acaatgcttt
     3421 actagtattt ttgacagatc ctttacaaaa cattattaat ttacaagtga aaatgcaaac
     3481 ggctcaagtt gcaaataatc gtttaaatga aattttcgcg attgattcag aaaaatcgaa
     3541 ccagtctttt gaatatatgc tttctgaaca attatttcaa caagatatca ctgtaaaaaa
     3601 cgtttcattc tcgtatagca tgaaggcccc aacgttgaac gaaatttcat gtacaattcc
     3661 ttcgcacagt aaaacggcta tagttggagt tagtggttct ggaaaatcaa ctcttgcaaa
     3721 gttatttgtt cgtttttatg aagcatcaga aggatcgatc cattatggaa acattaatca
     3781 cttagacatt ccttttcaga tattacgcga tcgagtgaca tatttgcctc aagagtcatt
     3841 tttctttagt ggaacaatca ttgagaatct tatattcggt ttagatagcc caccaagttt
     3901 cgaacaaata atagaagttt gtgagatagt ccaactaaat gaatttatta atcaacagcc
     3961 attacgttac gagacattgt tggaagaagg tggaaataat ctttcaggag ggcaacgaca
     4021 acgattagcc ttagcacggg ctttattaaa aaatccggac atattaattt tagatgaagc
     4081 aactagtgga ttagatacct tgttagaaca tgcaattatg aattatctcc tcaatttaaa
     4141 agaaaaaacg attattttta ttgcgcatca tttaccaata gcaaaagcat gtgatcaaat
     4201 tttagttctc catgaagggc atttggtaga aaaaggaacg cacgatgaac ttcgttttgc
     4261 cgatggtatc tatcaaaaat tatgggaaat ctaggagagg gaaacaattt atgcagaata
     4321 ataattggtt cgaacattct agtatctata ctaaacaaca tcatagcttc taccgttggg
     4381 tgttataccc tgtaattctt tttttatgta ttattggatt gtttttaacc tttgctaaaa
     4441 aagaggttgt tattcggaca acggctcaat tgaaccccga aaaaatagag aagttacaag
     4501 tgccattaga agctaaaata atggaaaact tcctaaaaga aaatcaattt gttcataagg
     4561 acgatgtgtt ggttagattg gattgttctt taatagagaa tgaaaaggct cagatagaac
     4621 aagaaaacca acgtattact caacaaataa agatggctca gctatttatt gaaagtataa
     4681 gtaaaggaaa aaatttgttt tcaacggatg acagttttgg ctacagtaat caattaaaga
     4741 gcatgttgtc agaaaaagaa tcactccgct acgctttgaa gcaaagtgaa ttaaatgatc
     4801 aaaagcaatt agaagtatac gaaaagacaa aaagacaact agaaaaacaa attgagagtt
     4861 cagatagtaa attacaagaa tggcaacaag tacaggtagc ttggagtaat aatcaatcat
     4921 taaaagattt ttcaaaagaa atgatggcaa actatgagaa ttggcaagaa caactaaata
     4981 atgtttctga tgatcaaaaa aatcaagtga aactgacaat ttcagcaagc ataaatgaac
     5041 aaattgagca actaaaaaaa gaagtagaac agtatcagtc agaaaaagct aaattagtta
     5101 aaccaactac ttctgagaat gacagaatta gtcaaacgga aaaaggaaag caagagctag
     5161 aacaagcaat aacaactgta aaacaaacag ttacagagct aaatgaaaaa caagagaaga
     5221 accagtcagt tatcaaaagt ttggacgatc aactttcaaa aggaatacta aaagctcctg
     5281 tatctggaac ggttcattta aatgaagaaa caaagggaca agcagaacta gccaaaggaa
     5341 cagtgcttgc agaaatttat ccgatccacg aaaaatcaaa aatgaaattt acagcacttt
     5401 tacctgcgaa tgaaagtacc tatattaaag aagggatgaa ggttcatttt aaattggatc
     5461 aaaaaggtgg ggctccgatt acgatcgacg gaactttaga tgaaatttca gaaaattcta
     5521 ctgccactga gaaaggtgtt ttttatgcag tgaaaggaac gttaaaaaca actcaatcgt
     5581 ttccgtttcg ttatggatta actggagagt tgtctttaat tgtaggagaa aaaacatatt
     5641 ggcaacaaat tgtcgatgtg tttctaaata aaaaataatt atttttacta agaatatagg
     5701 aggagtttat atgaataaaa gaagatggat tgctgtggga gtagcgtgcg gattattgtt
     5761 actatccatt atcgtcgcgc taattccagg aaaagataaa gaggaagcat caaatacaac
     5821 cctaacagga ataaataaga ttttttacgg atctaacgaa atcacagaag aaacgttaga
     5881 agaaggcgca tcaaataaaa agattgtcaa actatccgtc aatggagtta ttgcagatac
     5941 aggtgaaagc aatcttttta gtagggaaca atataaccat cagaattttt taacacagct
     6001 aaagaaaatt caagaagaca aagcggttaa gggagtgtta ttagaagtaa attcacctgg
     6061 gggaggaata tatgaaagtg ctgagattgc taaagaaatg gcaaaaatta aaaagctaga
     6121 tattcctatc tatactgcat ttaaaaatac agcggcaagt ggagggtact acatttctgc
     6181 tggttcagat aaaatatttg ctacggaaga aacaacaact ggatcaattg gtgtcattat
     6241 ttctggttta aattattctg gcttattaga aaaacttggc gtaactgatg cgacgtataa
     6301 aagtggtgca ttaaaagata tgatgtcacc tcaacacaaa ccttcagagg aagagaataa
     6361 agttatacag gaatttgtga tgagtgctta tgatcgtttt gtcaatgttg tagcaaaagg
     6421 aagaaatatg gatactaatg cagtgaaaga acttgcggat ggaagaattt atgatggtaa
     6481 ccaagcagta gaaaatgggt tggtggatca aattggttac tctgaagatg cattagattc
     6541 cctaaaaaaa gagaagaaat taactgatgc gacggttata gaatataaaa atgacacaac
     6601 tggctttgct tcttcttggt taggtaataa gattgctgaa tggcaagggc taaaggcaac
     6661 tacatcagat cgtgtaattt cagtttttga aaaacttgga ttttctgata ctccaaaacc
     6721 aatgtattac tatggagggc tataaaatga ataagaagag tggcattgaa actttgccta
     6781 attatgtatt tgcgggattt tggttacgat tttttgcttt tcttattgat ttaatttgta
     6841 ttagcatcat cacccatgct actataggtc tcttttacaa aatcatggga ctggaatctg
     6901 acaaatccat tattactttt tatggtatag cgtcattagc tatttattta acctattttg
     6961 tacttttgac taagctgaat tcaggacaaa caattgggaa aatgattttt gggataagag
     7021 tagtgagctt tgaggaaaaa gagctaagtt ggcaaactat ctttattcgt gagtgtatat
     7081 gtaggtttat attaaaaaat tttgtatttc aaattgggta tttggtggct gctttcacgc
     7141 ccaagaaaca acatattggg gattattttt ctaacacgtc agtggttgtt attcgttcat
     7201 tagaagctat taactatggt caaaaaattt ctaattctaa aacacttaat ctggagagaa
     7261 ttaaatgaat aagagaaaaa cagagtttat tttgatttta attagtggaa taacagggat
     7321 tttatgtagt cttttagcat tgatgggtgg tatgtttcta tcttctattg attcaactac
     7381 tttaaatact acagaactaa ccagtaagga tatagatatg ttagtcaatc taagcgggaa
     7441 tgttgtttta tttggaggaa ttaccctagt tttttcgata atgattttca taataggttt
     7501 tttagtaaga aaaaatcaaa aaacagtggt ctatggtatt agtttactca ttttatctat
     7561 tattccattt tttcttttaa cattattgtg ggtaattcct ggagttttag gtattattgc
     7621 gggattgatg cttctaataa gaaaatctta attaggcaat gtgtagacaa cgtgaaacga
     7681 ttaatcaatt aaaagctaaa tacgttttga gaaatgataa cttgtagtcg atggaagaca
     7741 tgtagataaa aaaactacgt gtcttttatt tattctaatt ttttcgaaaa aaagacactc
     7801 tctgattgtt gtattaatgg aaaaggggaa agcagcaagt aaatttagtt tttctattaa
     7861 atattcttca ctttttaacc ttttatagta cttagattta atcctatatg tttagagagt
     7921 tcattggcag catgattttg caaagcttct cgaattattg ttatattaga aactttactg
     7981 ttcataatta tgcttctttt ttaactcaaa gtccaaaggg ctacagtgga ctttgagtta
     8041 aaaataaaaa gccctagtta ttttgaaaaa taaaaaaaga acagcgtaaa tgttttcgaa
     8101 atgctttcgc accaacattt acctgttccg tgggtttggc acccaatggg ccgtgagggg
     8161 ctcgaacccg cgacccgctg attaagagtc agctgctcta ccaactgagc taacggcccg
     8221 tacaaaaagt acttaataat agtagcacta agaattttaa aatgcaagac tacatttaaa
     8281 gaaaatcaga aaaatcttta cttccttata ggaaatcatt catagattat tcacatttgc
     8341 tcgctattat ataagcatac caacaaacaa agttcttgca agagaacaaa ccctaacact
     8401 tttttcattt tttactcctt tcccgccgtc tccccggcgg gtctcctgtt cttaacgata
     8461 aaagacaaga aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc
     8521 acctagttaa agcaactaga ttgaatgttt atcataagtc tgatatcatg tttatgaaat
     8581 gaaaaaagga gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga
     8641 actagaaaca ttccgtctgc ctcactggga agaactccct gacttagaac tttatatgga
     8701 ccaagtcaga acgttagttg atcgttatct ttcacctgtg atacaaggag aaaagcatcc
     8761 attgcttacc tcttctatgg taaacaatta cgttaaacta ggtttgatac cagcaccagt
     8821 taaaaaaaga tataacaaag aacacgtagc atttttactt gcaatcacta cgttgaaaca
     8881 agtattgact attcctgaaa tcaaagaagg gatattgttc caaggaaaaa cagtaggaat
     8941 tcgtgaagcc tataatctat tttgcgatga acaagaagca gctgtgtgga tggtcagcca
     9001 attagcacaa ggtaaatctc atccgcaaaa attcgaccag gcaacccctg tagaatatat
     9061 tgctgtaaga gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt
     9121 tctagaaacc gaatatttaa aagaggagaa aacaaatgaa aaacaataaa atcgcaattt
     9181 tagtagattc aggtacggat gttccagaag aattgataaa aaaatacgag atgtatatga
     9241 tcccattgaa aattatttat aaagatcagg tatatacaga taaaattgac attacccctg
     9301 aagagatcta tcaacgtttg ccagaggaaa tccctggtac atctttgccg gatggtgagt
     9361 cgatcacaga aatctttgag caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa
     9421 caatttccag tggattgagc gggacatata atgtggttcg tttaattgca caacagcaag
     9481 aagaattgga agcatatgta ctagatacga aaaatattgg tattggtgca ggtttttctg
     9541 cgatccaagc agcaaaatgg ctggaagaag gagtagaatg ggatcaactg atcagtaatc
     9601 tgaatgagtt agtcgaaaga acaaaagttt tctttaatgt tgcgacattg gaatatcttc
     9661 aaaaaggtgg acggatcggt ttagttgctt ctattgtagg aaccgcttta aagcttaacc
     9721 caatcatctc atgtaatgaa gaagggattt attacacagt aggaaaagct aggggacgta
     9781 aaaaaagttt ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct
     9841 tcaacttagc tgttgcccat ggggatgcga aagaagaagc aattgaaatg gaagcgagac
     9901 ttaaaagaga gttcccacaa gcaaaccaaa tctactttgg ccagatttct ccggcattag
     9961 tagtccacac tggtccggga ttattgggtg tcggtgtaca agttttagaa ggataaatca
    10021 aaaaaaggaa acactgaggt gaaaatcagt gttttccttt tttattgaag ttagaacaaa
    10081 aaataataaa gtattttgca ctttcatttt aattttctct tttgttttat aggcaaagat
    10141 gaaacaaaag cagaaatacg gtataatgag aaggtatgac aataatatat aagaggtggc
    10201 tctttatgaa aaaagtttta gttgtcgatg atgagaagcc gatttcggat atcgtcaaat
    10261 tcaatttagc caaagaaggc tatgacgttt atactgcata tgatggggaa gaagccttgg
    10321 aaaaagttgc tgaggtagaa cctgatttga ttttacttga tttgatgcta cctaaaatgg
    10381 acggcttaga agttgctcgt gaggtacgca aaacttatga tatgccgatc attatggtaa
    10441 cagccaaaga ttctgaaatt gataaagtcc taggcttaga gttaggcgcc gatgattacg
    10501 tgaccaagcc attttccaac cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg
    10561 gtgccacggc tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg attggtgatt
    10621 tgacgatcca tccagaagct tacatggtga cgaaacgagg agaaacaatc gagttaacac
    10681 atcgtgaatt cgaactactc ttttacttgg cgaagcacct tggacaagtg atgacaagag
    10741 aacatttatt acaaacggtt tggggttatg attattttgg agatgtacga acagtggatg
    10801 taactgtacg tcgtttgcgt gaaaagatcg aagataatcc aagccatcca aattatttag
    10861 tgacacgtcg tggtgtcggt tactatttgc gtaacccaga acaggagtaa ttggatgaaa
    10921 agaaaagtcc gcttttttcg atcagtcaat tttaagatcg ctattacctt tattctgatt
    10981 ttattgatct cgatcgaaat tattggtgcc tactttatac gtggtttaga acgttccacg
    11041 atcaatacat ttattaaaga tatgaatcaa acggtggaat cattagcaac aacaatcagc
    11101 ccggaattga atcgaaaaga taatgcagat gatgaagagg taaatgcgaa tatcaaaaga
    11161 tttattgaaa acagtgcgac tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg
    11221 atcatccgag gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat
    11281 gttgatatca atgatttcac gactaaacgt tatgttgctt tagataacga cgataaacgg
    11341 gtgaatatca atgttcaacc gatcctgtcc ccgaccggcg atgccgttat cggggctctt
    11401 tacgtcaaaa gtaatatcga acaaaaatat tcggaaatca ataacacagc agtcattttc
    11461 tttactgcct cactaattgc ggcatttatt tcaatgatcg tatctgtttt agttgctcgt
    11521 tcgattacac agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac
    11581 tacagtcgga aagtcaaagt gcatggacaa gatgaacttg gacagctggc tgataccttc
    11641 aatcagttag ctgaacgtat tgaagaagct caagatacaa tggaagctga acgcaatcgt
    11701 ttggatagcg tgctatccca tatgacagac ggcgtcattg cgacagatcg caggggtaaa
    11761 gtcattacga tcaatgatat ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc
    11821 ggacaatcga ttttgacact tttagatatc gaagaagaat atacgttgcg gaaattatta
    11881 gaagatccaa acgaaatggt acttgaccgt tcaaccagta cattggaaag tgatcagatg
    11941 atcttgcgag tagacttttc tatgatccgt cgcgaatcag gttttatcag cggattagta
    12001 gctgtcttgc atgatgtgac agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc
    12061 tctaatgttt cccatgaact tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca
    12121 ttgagcgaag gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa ggtaacgcta
    12181 gatgaaacag accgaatgat tcgaatgatc aatgacctct taaatctatc tcgcatggat
    12241 acaggtaata cccagcttca attagaatat gtcaatttca acgagttagt gaattttgtt
    12301 cttgaccggt ttgatatgat ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa
    12361 tttacgcaaa gagatctttg ggtcgaagta gatacagata aaatcatcca agtagtcgat
    12421 aatatcatga ataacgcgat caagtattca ccagatgggg gaacgattac ttgtcgctta
    12481 ttagaaacgc ataataacgt gattttaagt atcaccgatc aaggattggg gattcctaaa
    12541 aaagatctga accgagtgtt cgagcggttc tatcgagtag ataaagcacg tgcaagagca
    12601 caaggtggga caggacttgg attagctatt tctagagaag tgattaaggc gcatcgaggc
    12661 gctatttggg cagagagcaa ggaaggaaaa gggtcgactt tttatatctc gctgccatat
    12721 gaaccttatg aggaggaatg gtgggaatga gaatctctga aaaagtcgtt cgtataggac
    12781 tcattttgat ggttgcgtta agcatatact tctcttatgc aatctggctg agcccagcag
    12841 gaaaaagttc aatcaacgta gatgagacaa acactcaaat gatcgagtct caaagcaatc
    12901 gaaaaacttc cgaagtcttt cttccgcttc atgttacatg gcaccattcg ggaatgatcc
    12961 aagaaaccaa cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat
    13021 ttggaaagct ttctgaagta gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc
    13081 tgaactctgg ttttgagttg gcctataatg cgcctttttt gctaagagaa tacaaggaag
    13141 catttgattt agatttagat gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta
    13201 ctagaataca attcgatcaa ggaaataaaa aaatccgatt tttgaattat tcgaaccatc
    13261 tcgtatatga agcaaatatt tcgttgaatt ggacagatat caataaagaa ttaacagcaa
    13321 aagacacaaa atggacagaa atgacaacag aaccatcgac gatcgacacg caatatgcta
    13381 cgaaacattc gatcaaactg aagaagtata gttatatcct gtcacagcaa ccctatacac
    13441 tttttagaaa tgcttttttc cagaaaccag atagcgtgaa aaataatgat gaaagcaatg
    13501 aattgatttt ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg caagtagttg
    13561 attttcgtgg agagctgcca gagaaaaaag aaaatgctag tgtattttct gaaagtttcc
    13621 cctatattag caaattaggc agttctttag ggaacctcag atattttgac cgcagtgaca
    13681 atacgatcga ttatcggata ttcgtggaag gcttcccggt ttttgggaca gacagcagag
    13741 gacagattgg tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa
    13801 ccagtttgaa tacgattcaa gtgccgatac cttcagacga agaagtagaa cttgcaagta
    13861 cgaatgaaat catccagcag ttaaaagcta agggagcaga tagcaacaag attggttcag
    13921 tgatcgttgg ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac
    13981 ctgaatggta tgtgaaatat aaagataact ggtattcagc taacgacctt ctggaaaaat
    14041 tacctggatt ggaggcgaat taattggatt tcaaaaaaat cgaatggata tttttcgtcg
    14101 cttttttagg gttgaatgtg tttttattca gcagttatca tgacgcggta taccaagaaa
    14161 acaatgtcat tcgctctaat cagactgaat caatccaaca acgtctggct agcgacgata
    14221 tcacttacac ggggaagatt tcgtctgaga acaagcaagg ctattatctt agcgcggagc
    14281 agaccaatct tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg
    14341 caagtggtgt gtttttagac ggtgatgttt tgacgaaatc tttatccagt tcagatgctg
    14401 aaaagaacgt gatcgatcca gataaaatta gtagttcctt ggaagatttt ttatctaaca
    14461 aagacaatat tttattcggt aatgattatg tgtacacgaa aaacttttct catttagaag
    14521 aagaaccact agaaatccaa gcaagccaag agtatgaagg catcccattc aatgatgata
    14581 cagcaaaaat cgttctttct ttagaaaaaa acgataatga ttatagcatc acgaaataca
    14641 tgcagacgca tttatctgat atcgagcagc tgcgcgaaaa gacggaactg tatacggaag
    14701 aggaagcaat caatacattg tacatcaata acaaaatacc aagaggatca aaaattttat
    14761 ggcgtcagct agcttattcc tgtattctga aagtccggga aaaaaatgtg tatgttcctg
    14821 tttggcatgt agcgatcgaa acaagcgata aagtggtcca aattgagagc gtcaacgctt
    14881 tcagcaatac gatcgtgaca aacaatactg taccaaaagt ggaagatcaa taaaaaagcg
    14941 agtataatga aaaaagttta caaagtaaga agggaagtag gagtcaaatg gctgaagaaa
    15001 aaccagcttt taaaatcagt atcctagcaa gtggaagttc gggtaattca ctttatatcg
    15061 aaagtgaaaa aaaacggctt ttggtcgatg caggacttag cggtaaaaaa atcacttcct
    15121 tgatggctca aattgatcgg acaccggacc aattagatgg aatacttgtg acgcatgaac
    15181 atcgagatca tatacatggc gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg
    15241 ccaatcagca gacttgggaa gcgatggaac cactgattgg aaatgtacca gtggaacaaa
    15301 aacatctatt tgagatgggg aaagtcagaa cattcggcga tatcgatatt gagagtttcg
    15361 gcgtctccca tgacgcagcg gctccgcaat tttatcgttt ccataaagaa aatcggtcat
    15421 tcgtcatgtt gacggacacc ggatattgca gtgattatat gcgaggtgtc attgaaaatg
    15481 ccgatggtta tttgatggaa agtaatcatg atcttgagat gttacgcatg ggaccttatc
    15541 cgtggaattt aaaacagcgt attttaggag atagaggaca tctttccaac gaagacggtg
    15601 cacttgtcat gactgatgtg atcggtaatc ggaccaaacg aatctatctt ggccatctta
    15661 gcaaggaaaa caatatgaaa gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa
    15721 aagattttgg cgttggacat gattttgaaa tctatgatac agatccagat gaagcaactg
    15781 agctattttc gatttaaaca gttcaatcaa taaaaatata aaaagcagaa gataaaatca
    15841 atcagggttg gttttatctt ctatttttat ttttttagca tattaaaaaa ataaccgtat
    15901 tatcgactga ctacactgat gtgctgcttt tcatgtttct catccagtat ctcatctact
    15961 aatgcaatcg cgtaatctgc catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa
    16021 taatcatcac ttaacaaata agtacctgta cgtttgccat gtggatcaaa taaagaagct
    16081 ggactgatat aagtccaatg tagattttta gattgtttca attttaacaa tccttcgtac
    16141 atattgaaag ctgttggata gaagggtgca tttttaggta cttggtcaat catacgagtc
    16201 gtcttttctg tatctaagaa taacgaagag gcacccccaa cgacaattaa gcgtgtatcg
    16261 gttccttcaa gcatctcaat caaatgggac aaagaagttt gatgcatttc ttcttttcct
    16321 ttgggagcgt tgaaagcatc aacgacaaca tcaaaagatg caagatcagt agttgtcaac
    16381 tcaaataaat ccttttgtat aaacggaaca tctgatggca atttttctgg atgacgcacg
    16441 attgccgtca cgtctaaatc tctggacaat gcttcgtcta aaataaatga acctgcatgg
    16501 ccagtagctc caatgagtgc aattttcata aaaaacctcc ttttttattc ctgtccattg
    16561 taagcagttg ctgaatattc gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc
    16621 tttcatggtt cgtgatcatt gggtatagtt aaattaagaa aactgattga taaaaataga
    16681 agttgaagga gataagaaat gaaagagtgg ttagatcgtg cctttttacc aatgataaca
    16741 tctgttgtga atcttagacc cttaactgct ttgcgtaatg gaatgactta cacgatccca
    16801 ataattattg ttggctcgct gtttttctta tttgctcatt ttcctgtgga ttacatcgct
    16861 cagtggatag acaaaacagg attttctatg tattttacac aagtatacca ggctacattt
    16921 gatgtgatag ccttttggat cgtttttgcc attgcatacg cttatgcaaa agaagaagac
    16981 atcgatcgct taccagtcgg cataacagcc ttgtctgctt atctactgct tattcagcca
    17041 ctaaatactc agatagatac tgtctggaca ggtgctgaag ggatactagg agcagttaca
    17101 actgggctca tcatcggatg gggctatgca cgtttagtaa gacttgcgaa agggtggaag
    17161 caaagagaga agatacctga aaatgtttgg cagtctttta cttcattgat tccaatcatt
    17221 gttatagtca cttttacgct tatcggttca gcggtttttc aacatttgtt cagaatgact
    17281 gttgttcaag gagtatggac atatgtccag cttcccattc aatcgttcat agacacgttg
    17341 tttggtgtgc ttttattagg tttttttgtt ccgtttttat ggttatttgg tcttcatgga
    17401 tctgcgatgg tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt agcaaatgca
    17461 gaaatcttag cctcaggaca agagttgacg gtggcaaatg gcggacatat cgttactcag
    17521 caatttctcg atcagttcat gacggttacg ggtgctggat tgacgttagg ggctgtattt
    17581 tttatgacgg tttttgcgaa atcgaagaaa tacagagaat taggaaatct tgctctttta
    17641 ccagcctttt tcaacatcaa tgaatcaatt attttttcca caccgatcgt gatgaacccg
    17701 atgatggctg ttccttttat tttcgcaccg gtcgtttcag gattgattac ctattccgct
    17761 ttatattttg gattcgttcc tttatttaca gcagtccaag tcccttggac aacaccgccg
    17821 atcctctcgg gtttcttagt aggcggagtg cctgcagcga ttttacaagg tatcgtgttg
    17881 tcaattagtt ttttcattta tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa
    17941 aaggaaagac agacaaaaaa tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa
    18001 atactcctgt cgctttagat tgggaaaatc accaatctag gatgacagga gtatttatag
    18061 tcatgtttat actagccctt tttggacagc taattgaaca agaacaacta atgtgttcat
    18121 tcctacgtgt gtgatcatcg atgtccagat ccgaccagtt gctttgtact ctaggctgaa
    18181 gaagaaacct aggaaaaagt agattagcag atgaccatca ttatgagcaa acgcaaacag
    18241 caaagagctg atcactgctc cgacccaaaa atttgaatat cgactgatga tccccagtat
    18301 actgcggcgg aatacgaact cttccatgat gggtccagca acagttgtcg ccaaaataaa
    18361 agcgggtgct tccatgatca tctggatgat attttgtgta ttctcggaag gggttgcttt
    18421 gccaaacaat acagattcta acatgacagc gatgctttgc agaatcaatg cgacaaagat
    18481 tccaaccaat ccataaataa caatctttgg agcagaagct ttttttaccg ctgattcgat
    18541 cgctaagggt tcacgttgct taaaataaaa aatagctagg acaacggcgc ctagtatata
    18601 catgactgca gttgctgtga tcaattcaga cgtagatttt acaagtccta tcgaagctag
    18661 gatcaagggt gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc tatatttttt
    18721 taaagacatg taattcctcc ttttaaggga taacaagcat gcaaggcaag cttatatcga
    18781 ttgatttcaa ttatatcata atttcaataa gcagctgtca gaataaaatt tcttttcttt
    18841 gatttaaaag cttgcaaaaa agagggagaa tgagtattat taaaaatgta ggttagcact
    18901 cgaaggttaa gagtgctaac agattaaaaa aagagttatc ggagggattt atcgtgttaa
    18961 aaccattagg tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa actgtcggag
    19021 gaatcgtttt agcttctgct gctaaagaga aaccccaaac tgggacagtt gttgcagtcg
    19081 gtgaaggccg tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc
    19141 aagttatgtt cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa gaatatctga
    19201 tcgtcgcagg aaaagatatc atggcgattg ttgagtaaat ttggaagata aaaaaataat
    19261 caaaaataat caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc
    19321 acgcgcagct atgttacgcg gggtagataa attagcagat acagtgaaag taacattagg
    19381 acctaaaggc cgaaatgtcg tactagaaaa atcttacggc tctccattga tcacaaatga
    19441 tggtgtgacg atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa
    19501 acttgtatca gaagtagcat caaaaacaaa tgatattgca ggagacggta caacgacagc
    19561 aactgttctg acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa
    19621 tcctctaggc attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca
    19681 taatatctca actgttgttg attcaaaaga agccattgca caagtagcag ctgtttcttc
    19741 gggttctgat aaagttggac atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg
    19801 tgtcatcaca atcgaagaat caaaagggat cgaaacagaa ctagatgttg tagaaggtat
    19861 gcagtttgat cgtggttact tatcacaata tatggtaaca gataacgaca aaatggaagc
    19921 tgttctagaa aacccatata tcttgatcac agacaaaaaa atctctaaca tccaagatat
    19981 tctgccattg ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta ttgctgacga
    20041 tgtagatggt gaagcattgc caactcttgt attgaacaaa atccgtggaa cattcaacgt
    20101 agtagctgta aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc
    20161 tattttgaca ggtggtacag taattactga cgatctaggt ttagaattga aagatgcaac
    20221 aatcgaaaac ttaggaaatg cttctaaagt tgttgtagat aaagacaata caacgatcgt
    20281 agaaggttct ggagaaaaag aagcaatcga agctcgtgtg caattgatca aaaaccaaat
    20341 tgctgaaaca acttctgatt ttgaccgcga aaaattacaa gaacgcttag ctaaattagc
    20401 aggtggtgta gctgttgtta aagttggtgc agctactgaa acagaattga aagaattaaa
    20461 attacgcatt gaagatgcat tgaatgctac acgtgccgca gtagaagaag gaatggtctc
    20521 aggtggtggt acagctctag tgaacgttat cagcaaagtt tctgctgtag aagcagaagg
    20581 agacgtggca acaggtatca agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat
    20641 cgctgaaaac gctggttatg aaggatcagt gattgttgat aaattgaaga acgtggaact
    20701 aggcactggt ttcaatgctg ctactggaga atgggtaaac atggttgaag caggtatcgt
    20761 agacccaact aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt
    20821 attaacgaca gaagcagtcg ttgcagataa accagaacca gcagcgccag cagctcctgc
    20881 aatggatcca tcaatgatgg gcggtatgat gtaggaatta aaagagaacc cttatattac
    20941 agggttctct tttctttttt tgttttattt taagtaaaag gggcaaaaaa ggggcagaaa
    21001 tatcaaagcc tatttagttt atcaattact tgttgttttg cttttttggt tacgtgattg
    21061 taaatagaca gagttgtatt tgcatcagaa tgtcctacac gctccataat ggctttaaga
    21121 ggtacgccta attcagataa tagcgaaaca tggctatgtc taaatatatg cgatgataga
    21181 tttttttcta attccaactc ttcttctact ttatggagta cagcgttaaa tgagtgcagc
    21241 gtaagcggag tgccatttgt agatataaat atatattgat cgggattggt gggtctacct
    21301 gcaagaatat tgtcagctat cacactttca atcaattctt ttgcacgatt ggataattgt
    21361 acttcacgtt gtgagtaagt attttttgga gttgttttta tggcgttatc catttttaca
    21421 gatgtgtaat ctaaggtccc attaatggaa attttaccct ctccatagtc cttcatttgc
    21481 aaagccagca attccccata tctcaaacca gtcaaatata gaaattcagc tattatgccg
    21541 tgtagttttc tgcgaggatt ggaatataac tgtttcagta tttgatcaat ttcttctttc
    21601 tccagatatt ttttatccat agaaagcttt cttttttctt cttccacttt tttaggatgg
    21661 attttaactg ctagagcagg gtttctttga atgtattttc tatcaattgc atagtttagc
    21721 ataacggata gagttgtttt tgtttgcttt gtgtagttca gtgagagatc accaaaagtg
    21781 tacatatctt caatcatctt attaatgagt gtctcatcaa tgtttctaac gattgtatca
    21841 tcgcttatgt gcttagaaac atgtttcatc atcattggaa ccttcaaata gctagtacgt
    21901 ttaacatgct gcttataata ttcataccat tctttataca gctcaccaaa agtgatatct
    21961 gatttattgt aattttcgag tgcttcttta atttttttat ctagaatttt ctgagctttt
    22021 ttccacgctt gtggtgaatt acttgtaagt gttgtagatt tttttcgtgt tttttctgta
    22081 taaggatcta catatctttc aataaacttg aattggccat ctttggtttg ttcaacccac
    22141 acttttaaca tctcctatca tttgctataa taggcataac aaatagacct atataggttt
    22201 gttttctaaa agcacgctct tactctggac ggtggggcgt gttttttatt tattatacag
    22261 ttgctttaaa aatagctgct ttttcaaatg atactaaagg agagaagtgg atttctattt
    22321 ctcccttttg gtttaacgtg aaatgagcag ttacatccat ttgttttcct ggagcaacag
    22381 atcccattgt attttcgttg gcatatgtct ctgatttttt gtcatctggt ccatatactt
    22441 ccacatctgt acctacaggg atatctgaat cgccatcatt ttttacggta taagtaattt
    22501 ttactacttg tgcaggttgg ttttcttcaa attgatttct ttcatcagtt agttctacac
    22561 tatttagtgt atattcagca tcaccaactt ttacagtgtc accaatctta tagaaggtat
    22621 cgcttttttc ttctttagaa gatgaagcag aggttgaaga tttagttact ttttcgccac
    22681 cattatcact tgctttatta ctatctgatc caccatttaa agcagaacca ataattataa
    22741 ttaaaattac tgctaatacc caaaaccata cttttttgta aaaaggttta ctaactttat
    22801 acatttttcc gtcttgaccc ataacttttt ttgccattta aatattcctc acttcttgtt
    22861 ataatatatt tgcgatctca gaaatgaggt atgagtccgt gtgccagcac gggctttttt
    22921 aatgttttgc tgaaatcggt ttttttaata attcttgatg tattcataca tattgccttg
    22981 cgtaagaata ttttttctta aaatagcatt ggcagacagc ataacaagtg tatctgagct
    23041 tattctgaat agaataatat tccataaatt tttcgaggtt aaactgagat tcatcagtca
    23101 gttcgttctc aatatagata tttaataaaa ttagaatagc tattttatct gcttctgttt
    23161 caaattttga gtgaaaagtt gtagaagtat cgtacaaaac tgaaaattca aaatgagagg
    23221 cgctgaaatg tgcaagttca tgagataaat gaaaggcttc tgcagtttcg ccatatagat
    23281 tttcattcaa aaaaatgatt ctaggcttcg gataatagaa gccaggctct ttcatttcca
    23341 tgtaaactac tttcaaattg tattcgctca acatttcttt caatttcaaa tacatacaac
    23401 ccatcactcc aactattcat tttcctctaa agctttagcg attgcaatcg ctttacgcat
    23461 tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc
    23521 cacatgttta ggggtttctt tttcttcgcg ccccagaaga tagtctacag agacatcgaa
    23581 atagtcagca atttcttgca aacgttctgt agaggggttt gagttcttaa gtctatataa
    23641 gacgttttta ccatagccta ggtcttcttc aactttattt aatgattttc cctgtttttt
    23701 cgctaactct tttattctct caaacgttat catatcaata ttcctttcgt tcaagaaaaa
    23761 atatttaact ttatttgtta aaattcgttg actattttaa caaaaggtgt taatatcatt
    23821 cttgtaaaca agtttaacaa ctaaaaagac aacaaaaaac actattgatt aataaatgcc
    23881 aaccgccaag aaagctttta aaatcaatgt ttatatgtct tatttaacta tgttctgatt
    23941 ttaacactgt atgttaattt agtcaacgca gttttttaaa aagttgttaa atttgtttac
    24001 gaatataaaa gaaaggagag aagaatatgg aaaaaacagt ctcggcaaaa atcgaagatt
    24061 tgaaattaga catactgaaa caagcaaaag tggcgatgga acacgcggta gataaagaag
    24121 actctgccat ggttgcagcc atagcagaga ttttagctca cgtttagtca tcgtttttac
    24181 ggtcttctgg aagctgacca tagataacag cataatgaat atctaaataa gtttgaacaa
    24241 tttcttttgg cgaaattgat tcgcctttag tagatacttg tgattcgtga tttagccatg
    24301 caacaactaa atccgcagcg atttcaaacg gcgttacact ttgtgtcagt tgccataaag
    24361 aatttcacaa tatgtatggt cgtggtaata ataccaaaga gcaatttgag gaatttatgg
    24421 aaattaaaaa atacaaaaaa tcatttatta aaggaggcta aacaatgaac aaggaaatga
    24481 gccaaacaat caaggtacaa aaaatgatag atgatctaac acatggaatt gatagccaag
    24541 ccgataaaat catcaaggaa ttgcaaggac aaaaagttaa ggatgcaaaa atgcttctca
    24601 agactatcaa ctttgaaatg aatccaacaa aaagaaagct cgccgatgtg ttggaagaaa
    24661 agttggcttc cgctatcaac gagcaagaat tactatttga aaccgatact tttaacagct
    24721 gatgttttat agatgacagt ttcatccgaa tctatatcat cgccgagaga aaagaatgtt
    24781 gcttttgaca aaaagccgat aagacctaca agcggtcttg atgttgcaag agtagatacc
    24841 ccaatatcac cttgcaaggt attcacgtaa atttgagaag aatagaacat tccatctttt
    24901 tcttcgatat ttgacgcatc atcgtatcca acgaccaaag tatcggcaaa tattttgatt
    24961 ttttctccat caagcatagt aattacaaat tctggtttca taaattttca cctccttaac
    25021 aattatttca gcctgtcaca ctgataagga aattatacca aagaaaggaa tgagaaatat
    25081 gaatacacca caaattttta atttcgagca aaacgaagtt cgaactattt tagtaaatga
    25141 tgaaccatat tttgtaggaa aagatgtagc agacgttcta ggatattcga atccgcaaaa
    25201 agctattcga gatcatgtgg acttggagga taagacgcag aacgattcgt tcaccgtcaa
    25261 tggaacagca gttgttttga ttaacgagtc aggcctttac agtttaatct taaaatcaaa
    25321 acttccctct gccaaaaaat ttaaacgttg ggtaacgagt gaagtccttc caacaattag
    25381 aaaaacaggt agctattcaa acgtacctca aagttttgca caagcattgc gtttagcagc
    25441 ggatttagaa gaaaagaacc aattactcga acaacaaatt gccgagtacg aaccaaagat
    25501 tagctactta gatacgattc tttcatcgac agatacggta gctacttctc aaattgcagc
    25561 tgattacgga atgtcggcaa ttgctctaaa caaattgcta aacgagttag gtgttcagca
    25621 taaagttagc ggacaatgga tactttaccg aaaacatatg aaccaaggat acacaaaatc
    25681 gcacacaagt gagataccga aagccgatgg cggcactaaa gttgtaatga ataccaaatg
    25741 gacacagaaa gggcgagtgt ttatttacaa cttattaatc gcagagggct attaccctca
    25801 aatggattta gaggaaattg gttagaaagg agttttagta tgactgacat tgcagaaatc
    25861 actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa gttcaaagtt cttaacttac
    25921 accatgcttg ctgaaagatt tggaattagc aaaagctact tatctttaat tttaaacggt
    25981 aaaaagactt ctgcagaagc aaacagaatt atagattcga ttatcactat gtacgaattg
    26041 tagagggagg taaagtcgtt ggaagataaa atcattctta caaaatcaga gcttcaagaa
    26101 atgttaaaca tcgagtatgg aacgataatt tttaggaggg ataataagtg aaaaaaccaa
    26161 cactttcaga gctgatagaa gctgctgaga aggcagtaaa accagacgac tggtaccgac
    26221 aaagtttaat cttggagaag ttccacggca tgtcaaaaac tactttagtt gaatactgca
    26281 aggaaatgga aacaattcct gaatttgcag aaggaatact tcgtccaggt cactcaacca
    26341 catttattca ttaccatact tttatttggt ttttaaagtg gaaagacgca aacaaatatc
    26401 gtgtaaaaag tttatcgcct tcagatgttt tgaaggaggc aagttaatgg gcaaattcaa
    26461 cagagcacta gtattcagcg caccgctaat catctacgct ttaggcctct ggggaagcag
    26521 acaagcgttg ataggtacga ttgtgtacat ggtttggatt tttatagggc tggatgaagc
    26581 tgagtacaaa acaaaaaagc cagtcgggag ggactgacta aaaaagtcta atgtctataa
    26641 gaaaggtttg acaataaatg aaagaattat tttttttaat aactttcatc attttttcta
    26701 tcagtgtttt ctacattaac ctttgcgttg ctagatttgc tttgaaaagt tttaggaaca
    26761 gcaaacgaaa atagagctat aaaagatacg aatgtagtag ctacgttaaa ttgctcttta
    26821 gaaaacgtca atgtcattgg aatgataatt gatatcgatg agaaaatctt tgcgataata
    26881 actatccatt tttcaattat aaacattttt aaattaagca ccattagaca aaatgtagtt
    26941 cctatataga ggataaatga agatagaaat aaacatatta gtccagtttt aaaaaaagag
    27001 aagtaatcta tgtgggcaaa ttctgttaat atgctttttt gatttaacac aatcaaacta
    27061 ctgttggaca aaaatgacag taaggaaaga gttgctccga ttactaaata aaaaatagtt
    27121 gttaacaata ttatattatc aatttttctg ctcatgtttt caccccacca gaattatatc
    27181 aaaaaggaga gaagaaataa tgcaagaatt agtaattttg aaaaataaag aagctgtgac
    27241 tacgagctta caagttgcag aaagcttcga taaaaagcac agacatgttt tgacggctat
    27301 tgatgaacta aaagaggggg ttgccgaaaa ttgggcagac ctattttggg aagatactta
    27361 tgttcatcct caaaataaac aatcataccg aattatttat atgaatagag acggcttctc
    27421 tctattagca atgggcttca ctggtaagaa agctttaagt tttaaacttc aatatattga
    27481 ggccttcaac aagatggaaa aagaattaaa agaccaattg ccatcaattc cagcaactaa
    27541 acgagaaatg gcattacttg ctctagctgc aaacgaagaa acgaatgaaa gagtagacgc
    27601 tatcgagtct gatttaaacg atttgaagaa caatcaactt ctagctgaac cagattaccg
    27661 aacaatttct aatatggttc gcaacaaaat tagagttatt tgtaatcagc agcatttaaa
    27721 tagcaaagca aaagcagaat tgtttaaaga cttgaatggc ggaattaagc gaatcactgg
    27781 agcagtagct agaaatcgta tcaaggcaaa acagttcgat gatgttatcg attttattaa
    27841 caattggatg ccttctacag cgacgatgac aattattaag caaatggagt tgttagagga
    27901 tgaatagagc tgaagcgcta aaaataggcg taataattgc taatcgctgg tggagacaca
    27961 ataaaccaaa catcctaagc caacaacata ttgataagca aaaagcatgg caacaaataa
    28021 aaaagtgact cagccgacca aagcaatgag tcacaaagaa aatacatcta aggagatgtt
    28081 accacatgaa aaaagaactt tccactctag atcaatattt gactgatcct agttggggca
    28141 aatcgaatat caaggaaaca agcaatcgaa aaatcagacg taatcttttg acggatgaag
    28201 aactagcatg tgatcaagac gatttgggaa attttgtgag tatttgggat catgtctatc
    28261 ttatccattt atcgaggaag tccaaaaaaa ctgaatatat ctatgtcatc gaagatggct
    28321 tgattgatgc gctagaagag tatgacagag ataacttgat tgatatctct tattacggat
    28381 caggtaagaa atacattgct gaaatggagg cagaatttga tgagtgaaaa ggaacaacct
    28441 ttgaaaaata gaagtgataa tacgcttttt aataccttat acaaaataaa tgtgaaagat
    28501 gttactgaaa aacgaaataa cctcacttat ctttcgtggg catgggcttg ggcagaagtt
    28561 agcaaagtgt gtgaggcagt agattatgaa atctatcatg atccagaaac atatctgccg
    28621 tatgtctttg ataagaaaac aggctatatg gtttttacca gtatcacagt caacggagta
    28681 aagcgtgaca tgtggttacc agtcatggat ggtgcaaaca aggcaatgaa agatgagcca
    28741 tatacctacg aagtcaatga ttatcagtgg aataacgaaa cgaagaaaaa agagattgtt
    28801 ggaaaaatcg aaaagcgagt tgaagcagca actatgtttg atatcaacaa aacgatcatg
    28861 cgctgtcttg taaaaaatct agcaatgttt gggctagggc tatatatatt tgctggcgaa
    28921 gatatgccag aagatgtctc gatgcttgaa ccagctactc aaagaagcaa aaagctattc
    28981 ttggatgctt tacaactggt tgctaacaag tacgaaaaat caattgatga agcaattgtt
    29041 gcattgactg atgcggcttc tataaccgct gatgacagta aatggaccaa gagagacttg
    29101 ggcattctaa aacgaggcgt taactggatt gaagatcagt acagagaaga aacaaaagag
    29161 aagtgatatg agtgtttaaa ccattgatcg attcatattc agcggttctg aaaaagttca
    29221 aaggaaaaga cataggtgca acgatcaatg aagaagtgaa cattgatcga ctaaagacga
    29281 tgtatgacgg atatgatggc gatcggatta ttgaagttcg atttattgat ccacgtcgat
    29341 ttactgtgca gcaacgaaac ttcatctatg cgctgatagg cgatattttc atcgatacag
    29401 gcatgccaac ggacttctgg aaggaattct tttacttccg ttttgaaggc gtcacagggc
    29461 gcaaaataag cctcaaagac gaatcgaata caactgtgag tgatgctaat gtcttagcaa
    29521 atatcatctt agatttcatc tttgaacatc atattccttt caaagaaggc tatgagattt
    29581 taccagcgaa tcaagaatat tacttctaca aatgcatcac aaaaagagtc tgctgcatct
    29641 gtggcaaaac aggagctgac atcgatcact ttgacaaagc gctaggaaga cgaaagcgca
    29701 aagaagttga tcattcagag tacacatttg cagcactctg caggattcat cacacggaga
    29761 aacacaatat aggtgtgatc aatttcaaaa ataaatatca aatcaaaggg atcaagttaa
    29821 accaggaaac aattaagaaa cttagaatag gagggtaaaa aatattgtct gacaacaaac
    29881 gctactacta tttaaaacta aaagagaatt ttttcgatag tgacgagatg gttctcttag
    29941 aaagtatgcc agatggctat atttattcta atattcttct caaactttat ttaagaagtc
    30001 taaaacacga aggtaagttg atgtttaatg acaggattcc atttaactct acaatgctcg
    30061 caactattac aagacattct gtaggagtcg tagaaaaagc ggtacaaatt ttccgtgatt
    30121 tgcagcttat tgacgtatta gataacggag caatttatat gtctgatata caaagtttca
    30181 ttggaaaatc ctcaactgaa gcagatagaa aaagagaata cagaaagaaa atagaagagg
    30241 caaaacggaa tttaataact ggaggacaag tgtcggacaa atgtccagac aaaactacac
    30301 cagagttaga gatagagata gagaaagata tagatataga taaagaagaa aagaaaggta
    30361 agtattctga cgaacactta cgccttgcta aaaaattgca aagtaattta actgaagatt
    30421 ttccaaaaga aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg
    30481 aagaaagaga taaagcgtct atagaagcga ttgagtatgt gatcaattgg ctacctacaa
    30541 atgaattttg gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc
    30601 tcaaattcga aatcaaagca gacaagaata atcataaaaa gcaaagtcaa aaactacagt
    30661 acagcaatcc tagtgaatat gacgacttgc caatttaaaa aggagatgca tcacatggaa
    30721 agcctagcaa atgctatgga gaaactaata agaagagtat tagtgcaaag cgggaaatgt
    30781 ccagaatgta gcgaaccttt gtatagttgg cgagctaaaa ataaggatgg ttcagaacgt
    30841 tgtaaaccaa catgcatgag ttgtggttat aaagcgttac gtgtgaaaga ggatatacag
    30901 accgaacgga tatataacga cagcttaaaa gcacgagcgt tgagtttttt tcaaaatggt
    30961 tcggtattaa cagataaaac tttgtttaaa tgcaaaatgg agaattatca cgtagtggac
    31021 caagaaacga aaattgcttt agaaagagct aaaagctttg taaatgatgt cttactgaac
    31081 catcctgcac atttcattct atcagggaaa tcaggaagcg gaaaaagcca cttgtcaatg
    31141 gcgacagctt gggaaatact tgagcgctca aattatgaca agaaaatact ttttataagc
    31201 tatcaagagt tattagagca aataaagttt tcttataaca atgctgaact gagaaaagaa
    31261 attgaaggat cgcttatagc cgatattaaa acaactgatt tggtggtttt tgacgatatt
    31321 ggagctgaat taggtagtgg ggtatcaaat agtaggcagt ttacaaacaa cacgttaaac
    31381 acgctcttag aagcaagaca gaacaaggca acgatcatca caacaaactt atctggtcct
    31441 gaactaagag aagcctacgg tgaaagaatt gtttctagga tatttaagaa ttcagaaggt
    31501 tatgcgctga aattccagca aacagcagac aagcgcataa aaccagtgaa aggtagtatc
    31561 gcatgaataa ataccgtaat aaaaaaactg ttcatcgagg tatcaagttt gattctatcg
    31621 cagaagcaga gtattacgat ctagccttgt ggcaagctga agcgaatggc tggaaagtaa
    31681 aacttcagga aagatttgag ctgatgccga aatttgaact agacggaaag aagtatcgca
    31741 agatcgagta tattcccgac ttcacatttt ataaaaacgg caaacttgtc aaagtcgtag
    31801 atgttaaagg aatgcagaca aaagacttta agatcaaggc aaagctgttt tgtcatcaat
    31861 atcaagtgcc gttgatatta gctaaaaaat atcggaatac gttcaaggaa gagcgttttt
    31921 aaagaggtgg tctatcatga caacagaaga agtgattcaa atgcgaattc gaagcattca
    31981 acgtgagatt gacgatctgg aacgaacaaa ggcagtgatg gtcaatgaaa cggcgagaaa
    32041 ggcaatcgat ttgcacatag agaacttaag aagggaaatt cgtagattgg aggaatgagc
    32101 gtggataaga aagcagcaat gaaacgaatc atcgaactga cacattctga gaattggcaa
    32161 gaagacaaag aaatagttgc agaagtccaa aagctcggta aatcaatgtg gactgaaaaa
    32221 accagacgga gaatgccgag aaaaattgca atctggcacg gtgaccgaat tctagtaaca
    32281 ggtaccgctg aacagttatc tgaaattact ggtctgagca aaaacatcat ctgggataga
    32341 gcgaagaata tggatattga ttctaaagga agacagttta ggtatgtgga ggagaaataa
    32401 tgaatctcat tacacaatac agtgatatca tcctcaagaa aatcatgatg aagattcaga
    32461 aagacaaaaa atcaaaagaa cgagcggaat tagttaagtt ggaaatggct gaaacagggg
    32521 caggagtgcg aagtagcagg cattggaagg cagcagcaaa cattgaattt tattacaacg
    32581 aaattcaaaa agggttcgat caaatgcgtg agctggatcg gcaaacaaat tggagcaaga
    32641 aacttcatca agatcgtttc aaatttgtag agaaatataa agagatatta gacgaataca
    32701 tggagggcag caaatgatac cgagatttcg atcgtggtac acaccattta aaggtaaaac
    32761 aattggacaa gaaatgaaat atgggcaagc aggaaggttg atcactcatg ctgaaatggc
    32821 tccagataaa tatgtgctta tgcaatccac aggactgaaa gataagaatg gcgtagaaat
    32881 atttgaaggg gatgtagttc tggttaatgt aagtaatggt tttgatcatt tagttaatga
    32941 aaaaacggtt gtacaagaat ccgagtttca ttccggatta gtttgtaaat cattaattag
    33001 tggaatggaa tatagaatat tcagccacca aaagatggga tacgaatatg aagtcaccgg
    33061 aaatatacac gagaatagcg agttattgga ggtagagtga ttatcttagc tactgactac
    33121 atcgattctt taaaagatga aaatgggaat gtcccagcat tgctaaattg tggaattaaa
    33181 agtaagaata gaatcatttt gttacggata tcggctgatt tagcaaaata tctttatctg
    33241 agcaagacag cagtatatta cactgcggct acacgttatc agtacaaggg aaaaacaatc
    33301 agtcaggatt attatgatcg tttaatcagt cctgacatac atggaaaatc aaaatcagcg
    33361 atcaagttat ttggcgcaat agagatatat gccgatgatt ttccaaattt atggttgaag
    33421 gaggaacagt aatgaaagac tggttagaag aagcaagcgc cttagttggc tgtttcataa
    33481 actgtgcgtt tatatctgta attattgcac tggtgggatt tgtatcattg aaagtgatta
    33541 tattgctttg gcagttaatt ttttaggagg aacagcgatg aataaacagg aagcaatcaa
    33601 aaagttagaa agtattaaag tgataggaaa tgatgcacta gctgcttgct ataacgagag
    33661 tataaatgca ggtattacgt taatgaaaaa aatagacgaa ccgaagaagc tagttattcc
    33721 acaactcgtg gccggttggc ttgagaaatc tacggaccct tttacgaaag ctgaaaaaat
    33781 agcgtattta atcaaatcta aagatggtga ttcatattat ttctgtgatt ggtttgtacg
    33841 agatggcata gtgacgcaag aacaaggaga agaattactc gcttgggcaa cgagacaatc
    33901 atatgaaaca ctattgagct tgtacaacgg ctacgaggtc gagaaagagc cgttgtacag
    33961 tgtaataatt gctggcgagt accttgtcaa ggagatcagt tgcagtaatg aagttaaact
    34021 agtatcaaca gcttacctgc cacaatacct tgcacaccac taccaactga ccgaaaaaca
    34081 gatcaaagca attgatgaaa gatactggcc atttgctgtg ccagtggaag aggtggcgga
    34141 aggatgagta gacagataat gctccatata ccagacggaa caaaggcaat gacgattacc
    34201 attgttgctg cacaaaaaga cggtgggttg gcgttgaaaa caaaaagcat tgataccaaa
    34261 cagattttag aaggcagaga tgtcgagata gagatcgatg aggaggaagc ggaatgaatg
    34321 ttcaagttta tttaaaaaga gatttggcca ggtttcctcg aagctgggat acaaattccc
    34381 acccatccga ggcagaagaa gagggctatc taatgtctgc taacatgata gaaattacaa
    34441 gagatgatgc ggatgagttt gataaaaaaa gatcatggta ctcggttccg aatccaatgt
    34501 atacggctgt aatagaagat tatcatatat ccgacagatt tattctgatt gatctagaaa
    34561 ggcctaagaa acgaattaaa cagtatagac gttgtggatt ttcaacaaag aaatggtgat
    34621 aacaaaacag aggtgaagaa aaatgaacaa cagacaccgc agagtagcaa aactaagaaa
    34681 tcaagaactg aatgtagcga aagtaaagtt cgaacgggag tatggtgttt ctgcagaaga
    34741 aacatataaa gtggcaagtc agtgtgttgc tgatgcaagt gatgctattc gtaagtttgg
    34801 aatttcgata ctagatatta aatgggagga tacagaatga agatttatgt agtaaagttt
    34861 ggaaatcaat tttacagaag cgatgaacgt tctatagggg ctaacacatt atccattgta
    34921 gacatactcc aaagcgcaag atggtttgat aatctcgaag aagctaacca agtttcacga
    34981 cgacttggtg gattaacgca agtatatgaa ctggtcactg tcgatcatga gggggtagag
    35041 taatgaaact aaaagacgga ttttacgcta gtagtcatgg tatcggcggt ttgatgctag
    35101 atatgccgac aaagaaccct aaaacacgtg agaaaccaaa attcaaagtc ggtgacatgg
    35161 ttcgctgtga agcagaagga tttatctatc catttcgtgg atatgtagaa aaggtattgt
    35221 cgaactcagc aatcattcgt attgaaaaca cgatggaatg tgacaagtgg ttagcgaaaa
    35281 gcaaagagaa tttagctgta gcgagattag ttgacatgga agtaatcgag agcaaataaa
    35341 aaagccggat cgctccgact aacataataa aacagacaag tttattatat cacataaagg
    35401 agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca agatgtaatg
    35461 cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc tctttgatag
    35521 atattaagtc tccaattatt acggatatgc cgaaggcgcc gaagcacggc aataaggcag
    35581 aggacgcgat cattcagatg atggatatag aagcagagag agatgcgatt ttagcggctt
    35641 tgatggctct tagtctgatt agccgtcaga tactctacta cagcttttgt gtgccagata
    35701 gcttctcaaa ctacagaatt agccgtgaag tgggttattc agaaagaagt atacaacgga
    35761 tgaagtcgga agctctaata gagtttgcag aagcatataa acacggaaga ataattgctt
    35821 ataaataatt tggcggtttt ttggcggaat gatggcggtt tttagctatt taccagtgat
    35881 attatggtag tgtcgaaaga ttagtgatag gtctgagaca aaataataat aaaaggaaca
    35941 tcgttttatt attgtttcac aattaagctt cgatagacag caacggaaat attaagaata
    36001 aggatgtgaa tttcaactcc ttctaaattg ttcttattat ctatcatccg ttgctgtcta
    36061 ttgttttttt aattattcac acgataacta aaggtgagtg aagagaaatg attccattaa
    36121 taatttcaat ttttgcgctc tgtcttaatg tctatatgat tggatttaaa aatgggcaaa
    36181 ataaaaaata gtagcagcca agaataattt tatagtgtca ctgtggcgga aagggtagac
    36241 gcttaaaaat aaggtcaata cgtcgaggga tagccttaac gttttatgat ttgaccatgc
    36301 aatgttcgat tcattgccag tgactttggc aaccgagggt tggaaatggg cgctcaaagt
    36361 acacgagcaa ggcgaggtcg atagtaatcg atggaatcgg tgtaggttgc ttgatagagc
    36421 tataactgca tctcgtttat gaggtgtagt ttttacagat tagatcactc tttgagtggt
    36481 ctttttattt tacacaaagg aggtaacaag aatgtataga ccacaatact tagaacagaa
    36541 gtatgaagta atcactgtgc aaaacggtaa cggtgagata gtacgaaagt atagaagacc
    36601 aataaagagc gatacataca aacgaaagga aagcaatgaa gttattccat tgtatggcaa
    36661 aagaatagct aagcattaaa taagattgcg aaaggagacg gaatatgacc gaggaattct
    36721 atagatggct attacagttg ataagagaag atcgtttagt taagttctat cagtctccta
    36781 aatggcgcag gcttagagag aaagcgatga aacgagatca ctatgaatgc caagagtgta
    36841 gaagactagg taagtatcat agagtagaaa acgttcatca tataaaggaa gtcaaggata
    36901 gacctgactt agctttagat ttagataatc ttatttgttt atgtgttgaa catcataatg
    36961 aagttcatgg cagatatctt acagcattag ataaacaaga gaagaagata gaaagctttg
    37021 ctaacttcga tgcaagtgaa aggtggtaag tgcatgatca tcaatgataa tggcagagag
    37081 tatgatacag aaaagattga agagtattca tcttatactc agggattaat taaacgtttg
    37141 atatacgttc gctatgtaga tattagggat ctgttatcag ataactgttg tagtaaatac
    37201 aaagtgaatc aagtaagaga agcgttgaat aaagataata acgtcgaaag aataaaaaat
    37261 gtttttggat atagtattga agagattaat tattacattg acttcgctga agctttcatt
    37321 ccgatggtga gataaccccc ccttaaaata aatcgcaaat tttttggggg tgatgaaacg
    37381 gagggggctg tcaggaaaag agattttttc gaactttatc atgaaaggag ggctaaaatg
    37441 tttaaaaacg aattgtctca aaatcgctac agagaaaaat tacgccgctc tttaataagc
    37501 caattggaaa gtcagaaaac aaatattgag ccattcttag ataatgttga tcgttatatc
    37561 agtttatggg aaacggcgat atcactggaa gaagatatat ccgagaacgg cattagactg
    37621 gagaatggta aaaagaatga atcagtagcg ttgcttgttt ctgtcaacaa acaaatggga
    37681 ttgatgttgg ataaacttgc cattactcct gaattggtag gtgaagcaaa tgaatcaatt
    37741 cctgagttat aagcatattg aaaattggtt caaagctata gaagaaggca ctatcaaggt
    37801 atgcaaagag caattattgc taaaaaatta tctagaagaa agagtcttta ctagagaaga
    37861 tatttacttc gataagcaga tggtagagga ttcaatcaat ataccagcac aatactttcc
    37921 attcgaatta attccgtggg aaaaatttct acaatgtttt atttatggtg ttcgatggaa
    37981 aaaagataaa acactagtgt tcaatagata tctttcatta atgggacgtg gtaatggtaa
    38041 aactggtttt gcttcttgga acaacttctt tctactaacc gctaaacacg gtattaaaaa
    38101 ttatgatatt gatatctatg ccaataatga aagccaagca aagactagtt ttgatgatgt
    38161 atttaaagta attaaagatc atcctgattt agataaaaaa gtatttaaag ctacgaagga
    38221 agttattcaa aatatcgcta caaacagcaa acttcgttat aacacggcaa atgctagaac
    38281 aaaagatggg aagcgaccag gtgcaaaccg ctttgatgaa attcacgaaa atgaagatta
    38341 ttcaatgata aatgtggcta cttctggtgg tggtaaaatt cgagattata gagaatttta
    38401 tgatacaact aatggtcatg ttcgtggtgg tccgcttgat gacattatag aagaatcaaa
    38461 aatgattctt tctggagaac ttggaattga caaggatgga gcagaatttt ctagtttgtt
    38521 tccatttatt tgtcgcttgg ataacgataa tgaagttgat gatcccgaca tgtgggaaaa
    38581 agcttgtcca actattaatt acaatgcaga tttaaaacgg aaaatgtttc aagaatactc
    38641 tcaaatgcaa cgtaatgctg gtttaagact tacgttcatg accaaacgaa tgaacagacc
    38701 tatggaagat acacgatttg ctgttgcttc atatgatgat gttctgcata cgaaagaaaa
    38761 agaatttcct gaaaaaatgg atgaagtgat aggaacagtc gattttgctg atagacgaga
    38821 ttttgccagc gttgggttgc taggaaaata cgataaagat gtttatttta cacaacatac
    38881 ttttatccac gaatcagccc ttcgattaca aaacatcaaa cgagaggtta tagatatttc
    38941 tatagatcaa ggaaaatcac agatcgttca tggaaaaaat atagaagctg attatattgt
    39001 aggttggttt cttgaaatga gtaataaata ttatattaaa aaaatcgcta tggatatgta
    39061 ccgtgcaaaa atattgaagc ccgctttaga agaagcaggt tttactgtgg aaattgttcg
    39121 aagcggatct gttacacatg gtatgttaaa agatctggtt gatgaccttt ttattaatca
    39181 acgtttattt tttggtgacg atgcgattat gcgttggtat tgcatgaatg tatatgaaga
    39241 gcatatttct aatggaaata tacgctatga aaaaatagaa cctgaaacta gaaaaacgga
    39301 tggctttttt tcattccttc atggtttgaa ttttttagat gatatttatg attctgctcc
    39361 tgtaacagtc acaaatagct cagtagaaaa tacaggaact ggatttactc ctctagtatt
    39421 ctaacttgaa aggaggtgag aaagtgggga tttttcaaaa ggcggtagga tacttcacaa
    39481 aaaaagcaac ggttacttta gaagaatact tttgtaaatt gcaagttgat tttgtgtatc
    39541 gaaaatttgc gattgaaact tgtattgatt tgattgcaaa tgcgatgagt aaagcggaat
    39601 tcaagtcata tgaagatgga aaaaataaaa agaatgatct ttactatagg ctgaatgtag
    39661 ctcctaataa gaaaaataat gcaacagaat ttagaaaaaa actgatcagg agattaatat
    39721 tctacaatga agtattgatc gtttctccgt ctaataattc tagcgaaata tttattgcgg
    39781 atagttggga tgtcacagaa tatgcattga aagatgatgt gttttctcaa gtgcaaatta
    39841 acaacatagt ccttgataga gaatttctag aaagtgatgt tatctatata aaatacgcag
    39901 atcaacaaat taggcaacta gtcgatgcgt attatcaagc gtatgggaaa ctcatttcta
    39961 gtgccatgaa tgtttacaag cgctctaacg ctcgtagata cgtactgaaa gggaatttat
    40021 tccgaccgca agacaataca acacaagatc aaatcaataa aatgatgaca tcacaattta
    40081 aggcttttat ggaagctgat aatgcaggtg cggtatttca attacaaaat gagtacacat
    40141 tagaagattt cagcggaaac ttccaaagca attcaagaga tataaaaaac ttaatagacg
    40201 acatctttga gatgacagca gcagcgtttc acgttccgaa aaacctacta aagggagaca
    40261 tgagtgggtt atcggatcaa gtggacgctt ttttaatgtt cgaaatcata ccgattgctg
    40321 aacttattca ggatgcgttt aacgctagtc tctatgaagt agaagaatac ttgtcaggga
    40381 attttgtacg tgtggataca actatgatca agattactag cttcaaagat ttggttgacg
    40441 ctattgatgt aggcattaga aatggagtat ttacaatcaa cgaaggaaga gaacgcgttg
    40501 gaaatgatcg ctctgataag gcgatggcag atgaaatatt tataactaaa aacaatcaac
    40561 aagtatcgaa aggaggtgag gcgaatgacg acaatgaaaa catttctagc agtaaagaat
    40621 gaaggcgcag taccgcaaat ttttattcag ggatttattg gttctagttg gttctttgaa
    40681 gggaatactg acaagggaat caaaaatatt ttggatagtc taggtgatca agaagaaatt
    40741 gaagtagtaa ttaattcaaa cggtggagac gtatttcaag ggattgctat tgggaactta
    40801 cttaagtcaa ataaagcaaa agttaacgtt gtgattaacg gcttagccgc tagtgctgct
    40861 tcaattatcg caatggctgg cgatactata aaaatctaca acaatgcaca attgatgatt
    40921 caccgcgctt ccacatacgg agaaggaaat gtcgatgact tccgtacgat tgctgaccaa
    40981 ctggaatcaa ttgataaatc ggtaaaggct tcatataaaa cacgattcaa tggcacagat
    41041 gaagcattgc aagaacttct tgaaaaagaa tcgtttatgg atgcagaaac agctttgagt
    41101 tatggattgg tcgatgaaat tatcgatgca gaaaatagct caggtactga agctaaaaaa
    41161 gaacaaagcg ttgaagaaat tttgaatgac gttaaagaaa aaagagcaga aaaaattgct
    41221 gcatttacag cagcattaaa taaaacattt ggacaaggag atgtaaaata atgacagtta
    41281 aaaatttaaa aggtgtaaca gctgcaagcg accaattgat gaaagctttt aaagatggta
    41341 acgaagaatc ttttagcgca gctatggtaa gcttatctaa ggaaattcag gataaaattt
    41401 tagaagaagc aacagcaaaa aatcaagatc aattagtatt aatgaaccgt ggtcagcgtg
    41461 tattaactac acaagaaaca aaattctata acgaagtagt gaaaaacgaa ggttttgcag
    41521 gggtcgaaga attagtgcca gctactgtat ttgaacgtgt atttgaagat ttagaacaat
    41581 ctcatccact attgcaaaaa attacttttg ttaacacaac tggtgtaaca gaatggattg
    41641 tgtcacgtgg agtcaatcca gcatggtggg gtaaactttg cgaagctgtt aaaaaagttt
    41701 tagataatgg ctttgatgta attaacatga agcagttcaa gctatcaggt tatattcctg
    41761 tatgtaaggc aatgcttgat ttaggtccag tatggttaga tcgttatgtc cgtactgttt
    41821 tagtagaatc attgagaatt gcattagaac aagcaattgt tgatggtact ggtaaagata
    41881 tgccagtcgg aatgatgcgt gacatgagca aacaaactag cggagaatat gctgaaaaaa
    41941 aagcagaacc tattacagct ttagatgctg taactatggg cggtttgatg gcgcgactat
    42001 caaaattcaa tatcgaaggt gtgaatgatc cgatttatcg taatgtgaat ccttctgatg
    42061 tggtcctaat tgtgaatcca actgattact ggtctaaagt attcccagct aagactgtac
    42121 taactgctaa tggagaatac gtacaagtat tgccagtacc agtttcagat ttgcagtcaa
    42181 cggctgtgcc agaaggaaaa gcagttattg gggtagcctc agattacttc atgggtgtag
    42241 gatctacact aaaaattgaa gcttcagatg aataccattt tgttgaagac gaacgcattt
    42301 atctagctaa acaatatgca aacggtcaac ctaaacgtaa cgatagtttc attgtattag
    42361 atattagcgc tttgggaact actactacaa ctacaaaacc aacaaccaca acaactacaa
    42421 cacaagcgta ggtgatcaga atgaagtata ttctttgtca gccggcaatc aatcggttta
    42481 aatgggagct tgaagtttgt ttaactaatc tgaagaaact aggaatcaaa gatatcgtat
    42541 tgcttttcag cagacacgat gatcagattc ctattttttt tgagaaggaa tatggtgttg
    42601 aagttcatgt gtacgatgat ctgcgggacg acaaagagta tattccttcg attaaaccat
    42661 atttatggtg gaaatattta gaagaagatc attcgcgtga ggacgaccga tatttctata
    42721 tcgattcgga tgtcattttc aataaaagaa ttaatttgcg caaattgcct tctaaagatg
    42781 atgtttggta ttgtagcgac tgctgtagtt atctaagtct tgattatatt agaagctgtg
    42841 aaaacggaga aaatattcta aaagatatgg caaacattgt aaatgttaca gtagaatctt
    42901 tggaaactat aaacactaat tcaggaggcg cacagtgggt tattaaccgc cctaaagcga
    42961 attattggaa aaaggtttat ctggattcta atcggctata tcgctacctt agagggcaaa
    43021 aaacaaatat aaaaatctgg acagccgaga tgtgggcaca gctttggaac atgatgtatt
    43081 tcaatattgg tcctaaagtt cacgaggaat tagacttttg ttttgctact gatccaatag
    43141 aaaaagttaa agaagtaaaa atcttacaca atgctggagt aacaacaaac gatgaagatt
    43201 tatttttcaa agggagatac gtgacttcta cgccttttga tgaagattta tcatttgtaa
    43261 acaagaaaaa atgctcttac gcatatgtta aagcaattaa ggcggtggtt agatgacgcc
    43321 tgaacaagtg actgaagaat tgctaatagc tgtgaaggat aatatttacg ttacctggaa
    43381 cgaagaagat gagtcaatta aaaagatgat agctaaaaat gctgtttatc ttcaaagtaa
    43441 agtgagtaca acactttctt tttctcctga aagcttagaa tacggattgc taatcgaaag
    43501 atgtagatac gactggaatc gtgctttaga tgagtttgaa caaaatttcg ctagtgagtt
    43561 attaggtttc attcaacatt atgcgctaca agaatatatt gcaggtgatg tgaatggcga
    43621 ataatcgtag actcgaagaa acattcaacg atggttggtt aaagattttg acgcaaacta
    43681 ccaaaagaaa tgaactagga aaaaagattg gtgtagaaga tacagaaatc acttctttaa
    43741 aatttagaaa tctttccatg agagatagtg atataacagc tatggatgcg atgggatcga
    43801 aattaactaa gaaagtaaag actccatttc atccaatcgc caagaaattt aataaagatc
    43861 aatattttat cgtaatcgat agtatgcgtt acaacgttat ctatgccgat tacgataatt
    43921 tttatatcta tttttatctt gaaagtgtgg gtgaatatgg tgattgataa ttctaaagaa
    43981 aaagaacgtt taaataagca aatttctgct atcaaaactt ccttagaaga gcattttggc
    44041 ctcaaactct ttcaagactc cgtaggcgag gatgagctac ctgatgattt taattacttc
    44101 attctcgaaa caggagaaat agaaatgatc actgagccaa aatatagcgt gggtcaaaat
    44161 ctatatctaa ctttctattc agaaaataga gaagatttaa caggagattc actagatatt
    44221 atttcattga ttcaaaatcg ttcgattcgt tttcagagaa tggatcccaa tcatttaaaa
    44281 ctagagaacc aagatcgcta tatcgatcaa ttggtattta cgtttagacg attattgaag
    44341 agtgattgtc atggctaaaa atagttggga gctaaaaata aatggacatg atgaacttct
    44401 tgtgcggatg gaacgctatt caagcgagag cgaacgactg attaacgaag cattgaaatc
    44461 aaaaggttcg gctattgcag tggataggat tacagaaaaa attcctgttt ctgaagcaga
    44521 tttaagaaga gggcaccaac acgcaaaaaa tagtcgtcca cttaagactc aatacattaa
    44581 tttgggtttc atcattagac ctacaagaaa atttgagtat ttaaaatatc ctgatttggg
    44641 gataggtact tctaaaagaa atcagccgga cgaatttatg agaagaggat taggtcttgc
    44701 acttgatcca attacagaac ttctgattcg tcaattcgat aaattaaata aataggagga
    44761 acaacaatgg ctaaaacaac aacagtaaca acttttgaca atataagtat taaaagaatt
    44821 tcttttaatt ttaagaatgc aacaaatgca atttcaactg attgtaatgg acaattagat
    44881 ggcgaaacag aaatgcagag aattgtaaaa aaatgcggtt caacagaagt aaaatcgaaa
    44941 tctaaaccaa tcaatatgac ggtaacaatt actgcacatg taccgatgga agtttatcga
    45001 cgcttcaatg ggttgaaaca agatgaacgt attaaatcgg gaatttactc ttatggtcct
    45061 gattccgtag gggaagattt ctctcttgct gcagagatcg tggatgattt cgaagaaaaa
    45121 aataagctga ttggtatgtt agcatgcact tcgaatacag gattaacatt ctctattgaa
    45181 aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa aagttatgca agatgaattt
    45241 ggtaaatttt atcatgaagc aattgttgca gaacttgaag aagacttaac agatcaatgg
    45301 atgacgaatc tatctgctga tgtgattaaa aaaacttcag ttgtgacaac tacggccact
    45361 caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa gattactcaa aaattgaact
    45421 aaacgatgga acaattttga atttagaacc taaactgaat atcaagaaat tattgatgat
    45481 caatagagat tttaacacag acgagtttgc aaaaatgtcg atgggaaaag gctctatgga
    45541 tattacagtt attcaaggtg caaaagccgt atatgtagct tatcgtcaag cgaacatggt
    45601 cgattacatt tcattcgatg aatttataga taaatgagat tttgatatgg aggttgcagt
    45661 agctgtatac agtactatga tgttcaaaca agcacgtgat gcctatcaaa aagaattcga
    45721 aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc caaagctctt agttgaaacg
    45781 tgggtcgatg tctattcgat gttgaccgac gttttttcta tgccttcaga tttggtttta
    45841 agcgatatct gtttagatga cattttgcaa atggcttaca acaagagcgc ttatgaagga
    45901 tggaagaact atgcaataaa tcaatcccag aaaaactaaa ggaaaggagg taaaaaatgg
    45961 ctaaaaagag aacggaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta
    46021 cgttaaaaga aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag
    46081 ctcaattgca acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag
    46141 aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac tagccaacgt ttagccaatg
    46201 cgaaaaaata ttatggagaa aattccaccg aagttcagaa acttgaaaga gaactgatta
    46261 atcaacaaac agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatgcactag
    46321 ctcaagcaaa aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagcgaac
    46381 aaaaaaatgt tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagcaa
    46441 ctgctggtca atcagcttct gaagccgaga aattagcgaa agcccaagaa tatgtttctc
    46501 aacaatctga aaatgcggag aaaacgatag atatcctaag acgacagtta gaagctacac
    46561 agtctgaatt tggcgctaca tccacagaag caatgcagat ggaagcgaag cttaatgatg
    46621 ctgaacgtga atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg
    46681 acgatatcgg aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg
    46741 acattggcga taagcttaca gaattaggga aacaagcagt ggactctgct aacagtgtag
    46801 gtagttccca gagtaagata caagctaatt ttggtttgtc taaacaagag gctgaagaat
    46861 taacgaatgt agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca
    46921 cagatgcatt gattttggta aagcgtaatt taggagattt aaataatcaa gatttacaaa
    46981 atatcacgga acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt
    47041 tacgtggtgt aaatggctta atggtcaatt tcggcttgag tgctcaagat gcaatggatt
    47101 taatggtttc gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg
    47161 cagaatatag ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc
    47221 ttcaaaatgg tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg
    47281 aaatgggaat atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa
    47341 gtactagaaa agcttttgaa gagtggaaaa atggcggagg aacacaaaaa gacgttatta
    47401 attccatgat tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt
    47461 cgacaatttg gtctgcgctt ggcgaagata atgcgatgaa agttgtccaa tctttaactg
    47521 atgttaacca tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta
    47581 ctactccgtt acaagaatta aacggaaaaa tagctgaatt aaaggattca ttagctccta
    47641 taggcaacac aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg
    47701 atgcgtttaa taatcttcca caaccagtac aggattatgc cgtagcgatt ggcggattga
    47761 ctgctgcatt tactttatta atgccaataa tagttggctt catggctcta ggtggtccta
    47821 ctacattaat aataggagca gttattactg ctattgctgg agttatagca attataaaaa
    47881 actggggcgc aattactgac tggtttaagg gaatatggag taaattcact gattggttgg
    47941 gtggtacttg ggaaagtata aaagaaggtg cctcatcagt ttgggatgga gttaaagaaa
    48001 cctggtctgg atttgtagat tgggttcaag atatttggca aggagtttct gattggtttg
    48061 gagagctatg gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaaa
    48121 cttggcaaac attcgttgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt
    48181 cgattatttg ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat
    48241 tgattcaagc tggtattaat attatcgtgg gtatttttga tgtagctgga cagttattag
    48301 gcgcagcttg gcaagctgtt tggacaccta tttctgattt ccttaaaaat acttgggata
    48361 ctatgacaca atggataagc atcgcttgga acggaattgt aactacattc catactatat
    48421 ttgatccagt agtggcatgg tggaatggta tatggacatc tattagtact acggcttcaa
    48481 atatttggaa ttcaattagt gcaacagctt ctagtatttg gaacagtatc aagaatacaa
    48541 tcactagctt ggtacaagca gctgctacag taattcaaaa tatttggtca actgtatcta
    48601 gttggttagg tggaatttgg aattcaatca gctctacagc atcaaatatc tggaatagtg
    48661 tgactagtag tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga
    48721 atagtatatc ttcgtggatc agcggaattt ggaacggtat caaaaacact gctttgaatc
    48781 tttggaatgg aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt
    48841 caagcggttg gtccaatcta acaggtattg tttccgacat attcaataat gttaaaagta
    48901 caattgctaa catttgggaa ggtatcaaaa agactgttag cgctccgatt gattggatta
    48961 gagataaaat cagtggcatc tttgataatt tgaatatttc gataccacat attccgttac
    49021 cacattttaa attgagtggg gaattcaatc cattgaaggg aaaaatccca acgttgggtg
    49081 ttgattggta tgcgaaaggt agtgtgttta attctccgaa tattatcggt gtcggtgaag
    49141 caggacctga agcagtttta cctttgaaaa gatctgtttt gcaagaaatt ggtgatcgta
    49201 tcttgagtag cacatcagtt tcatctaggg cacaaacgat tcaacctgtg aataactacg
    49261 aattcaattt cacaattgat ggtaacgcag atgaggttac tatgaagcaa acaactcaac
    49321 aaatcattga tagcattaca aaaattcaaa atgataatgc ttcggcatgg cgttaaacag
    49381 gagagtattt ctcctgtttt tttagtatta aaaaggatgt gaaaaaatga ctgattgtat
    49441 acattctata atcgatggat ttcctgatta tttgcataaa ttggctttag ctgaaagacc
    49501 aaccatacct tctccaaaaa gacagagagt tgaaacttct gttttaggta ggttaggtgg
    49561 cttagtacaa gattactcgt ttgaagatat gtcgtttaca ttgcactata actatttaga
    49621 ggatgtggaa gaccatcaag cgttcaagca atcgttttat atcatgcgtc attggttaaa
    49681 ctatgcaaag aaattagaat tctctgacga tcccaacgtc tattacgtta tccagactat
    49741 cgatattggg gatgcagaaa acgatattgt tgaatgggga gagttcgatg taaatattac
    49801 tgcgaaacca ttcgcaagag ttcaagaaga tgtacctata accgtagata aaccacagtc
    49861 atttaacttg ctgaataata gtttagaaga aagttttcca aagattatca tcactccttc
    49921 agctacttca tgccagttca tcttaaatga ttatgtgttt agtttcgaag gcttagtagc
    49981 aggaactgat gtagtcattg atagtgattt gatgctttgc tacgaagagc aatcggacgg
    50041 agatatttta gatcggtcca acaaaatgaa gaccatgcaa tatccaacgt tgcaagtgga
    50101 tattaatcat tttaattgta ctggtttgag caaaatacaa atttatcgta acgggttaag
    50161 gtaggtgaaa tagatgatcg ataatttaat aactatttac gataaaaatg acgcgaataa
    50221 tttagctgaa catttatatg atacgcaagg tttaggcgct ttgtcagact ggttaacagc
    50281 tactgttagc aataaactaa acggagccga gatatttcag ggtacttatt caataaacgg
    50341 aactaatgca gatttgattg tagaaggacg tattattcag tgttatgtag atgaaaatcg
    50401 agcaaaacag cgtctacgga tttattatgc aaaaacttct gtaataggca atacgataga
    50461 agtaaaagct gaacctattt tcaatgatat aagaaaatcg gtgttgaata aatatgacag
    50521 cggaacagaa aagatcactg ctactcaggc atggcaaaac gcaaaagttt tagcgaaacc
    50581 agctatccct tcgcagtttt ctttctcgtc attagtagat acgcttgcta atgtgaagat
    50641 agaaaaggcg aattttttag aattctttgg tggaaaagag ggatctattc tagatcgatt
    50701 tcatggtgag tttctaaaag ataataacac attacgtcat gaaaaaagtc taggtacgga
    50761 tcataaaatc aaagcgattt atactaaaaa cttaactggt cttgacttag agattgatgc
    50821 tcaaagtgtt ttagttggag tttatccatt cattagcagc tcttcagaag gagaagacga
    50881 gatcactcta ccagaagaag ttattttcac ggattacgtg gatgattatc ctgctggata
    50941 tgtttctttt gttgatttta aagacaaagc gactgatgta gccacattaa gggaagctgc
    51001 taaagactgg ttgaaaacaa acatagataa acaaaaacca caagtgagtg gttcgattga
    51061 attagtacca ttgaggcatc aaagaggcta tgaaaaattt gttgatctag aaaaagtttc
    51121 gatgggtgac ggagtagatg tgtatcatcc acagttaaaa gtgaatatgt cagcgagaat
    51181 tgtggaatat acgtttaatg ttctaaccaa ttcatacgat aaattagttg taggaaacgt
    51241 caaaacaaac ttcttagaaa acacagagaa taatgtcagc aatttgatta atgatgccat
    51301 tgatcaattg aaaaatggtg gcgaaatcag tgatttaatc aatgatattg tagatcatca
    51361 aactgatatg attactggtc aaaatggtgg ttacgtttta ttagatccta aagaagcccc
    51421 tagtcgtatt ttgattatgg acacaccaga taagaatact gcacggaacg ttttgcaaat
    51481 caacaacgct ggtattggtt tctctaaaac tggcattaat ggaacgtatg acaccgcatg
    51541 gacgttagat ggcggattca atgcctcgtt tattacagct ggtgagatag tagggattac
    51601 tattagaggt actacattaa ttagtgatgg cactgattat agaacaagta tagctaatgg
    51661 caaaatgact tggtactcaa aaaaagttaa caaagatatt atggagctag aagcacgtga
    51721 ttatgtaagt gctgatgcag gtattgtatc atacaccatg aaaactggtg gtggtttcat
    51781 gattagaaat ccacagggga atttggtttt tagtacgtgg gataatggta ataacaaacc
    51841 gtttttatct tttggtgcgc ccaatttcag gtatagcaat gctagttata taactgatgg
    51901 cgatggtagt tctttaagca ttaatggtag tgcgggcagt tcatgggagt ttaaagtagc
    51961 tggtaggact atgaaattta ctagtgatgg tatgctaacg ttgccaggtt gtttttttgg
    52021 ttcatgggaa gatggaaaac ttgctaggtt tgaacaatca acggtacaag tatataaaaa
    52081 ttttactgtt agaggtacta aaaactcaac cgtaccaaca gaacattatg gacaacgact
    52141 attaaacgct tatgaaactc cagaatatta tttcgctgat tatggggaag ccgttacagg
    52201 tgatgatggt aaagttcgtg ttgatattga tcctatgttt gccgagacag taaatctaag
    52261 tcgatatatg acacatgtga cacctacaga gctagttttg tgtgctgtta ctcatgaaga
    52321 tattgaccat ttcatcattg aaactagtaa gccaaacgta ttggttagat ggaatttagt
    52381 ggcacatcga ctagggtatg aagatgttag attaaaagag gatacagcat atgatagcac
    52441 agtgcttgac caaaaacgtt tttaaaacga agacaaggag gtatataaat ggctagcagt
    52501 ttatataatt tggctttaga tttcagcaaa gaattaaact acaccaaagc tattatggct
    52561 cgtcaaggtg acaaaggaat tacggtgact gttaaaccgt ttttaaatgg cttgcagatg
    52621 gatacgagtg gcggaacctt tacattaaaa ggaacaacac catctaaccg ttacgtagat
    52681 agtgtcgcta ctagtataac tagcgaagaa gtcacatttt ctcttgatgg aacatttatg
    52741 agtgaagcag gatactataa acactgctac gtagaatata gaaaagataa tcaaatttta
    52801 acgacgcaag atatcatttt tttctcacta ggagtgtctg acatttcgca aggccaagcc
    52861 gatgaatatg tttcgcaatt agaagaattg attcgaaagt ataacgaaac ttttgatgct
    52921 tttctggctg aaattaaagg tagaatggat agcttaaata aacagattac tgatttaact
    52981 ggtcaagcta aaacactaca agacaagtta gatgctctga aagaagaaat ttctaaatta
    53041 ggtaacttac atgtgatgta ctccaacagc atcgacttcg ggggctatga ttatagtggg
    53101 aatccgaatt taatgtccaa actaaaatcg agcgatttta acgttggtta ccacgggtca
    53161 ctaactaatg ttaacggaat gttacatttt atgtctgatg gtacagggga cattgatatg
    53221 tttacgcgta ttaatacacc tcagcttgct agtgggaaaa cctatactct gagtgcgaaa
    53281 gttcgatttg atgaaggaac tacaggagct attgataaat tacgtttggt gtatcgtaca
    53341 tcaccaggag aaaagatatt attggaagca aatagtacaa atattacaac agatgatgta
    53401 gggaaagaaa taacaatcaa aggtacagct aacgttaatt atcaaatcac aaatttagat
    53461 cgattttata ttagtgttag tttttctaac agggataaaa taaatggtgg atttaaattg
    53521 tacgacatca aaatcgaaga aggctcaaca gccactccgt atcagccaaa cttactagat
    53581 gacccttact gggtaggtaa aacatcgttg ggtaagaata ttgcaaataa aggtacgacg
    53641 ttcccaatta aatctagcgc gtacgaaata tataaaggta acacggaaga agagcttata
    53701 ataggtcaaa cgtatactat cacgcttaaa ggaacaaaac ccgcaagtca aacctttgta
    53761 gcatataatt attggaacgt taattttgga gatctaaaac cagttgaggg attgacagac
    53821 gtatggtctc taacattcac accaacgaaa cttgaaccgg gtttgcctaa agaccttcgc
    53881 atttttcagt cgcctaaaga aacggccggc gcatgccgaa ttgactggct caagatcgaa
    53941 aaaggcgaca cacgaacccc gaatattgag caatataaat accgaggaat cggcatgcga
    54001 gactcaaaca atccaaaaga ttatgtatgg gatctagaac ctagctatgt tgagaataac
    54061 ctagctacag atatcaaggt tgctgaaatc acaggtaaag caaacaatta taccgatggg
    54121 aaagtatcgg agattaattc gcagttgact gcttcaatta atgaagtaga caccacagct
    54181 aaggatgctc aaacaaaagc gaatgctaat gcgactgcta tagatgaatt agacaataag
    54241 atcgatgaac gcattaatga tacagctact accacattaa cagttacaaa cgggaatacc
    54301 ggatcagcaa agctttatcg tgaaggaaaa acagtttcta tatattttgt ggctttaaac
    54361 ggaaaacgca gtggtggaaa tgattcaacg atactaacaa ttccagaagg ctatcggcca
    54421 ccaattagtt ttgagcaact ggttggctcg atagaccgtt ctactttgaa cagtgctcag
    54481 ttatctattg gtgcagatgg agccattaaa tggcgaagaa actcaagtta tggatcggat
    54541 tatacctttg caattactta cacgatttag aaaagcgtga atcgatatga aggcagcata
    54601 tagaccaatt gaaccttacg gattcgagca aatcattgtg aatgatgaag aacatttacc
    54661 ggaagaatgc acagaagtcg aaccaccgat tccaaattgg aaaccgaaat tcaattactg
    54721 ggagggaaat aaatgaaaaa catttggaaa tatggacgta ctggcggaga gtacgcagga
    54781 aaagtattgg acgacatgct tgtatccgtt ccttacacag atcagccacc gcttgaagga
    54841 attcgtgctg atggcgaacc gttaacaatc gctgatcaaa tgtttgatcc taaattgaat
    54901 caatggatta ttttagcgaa cgcactagat cacaacgatt taaacaatct caaagcaatg
    54961 tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga tcaatgccaa actcatgcta
    55021 agcgatgtag caattaaaca ggaaaatact gcattgaaag aaaaagcgga tagtttagca
    55081 caaatcaatt caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt
    55141 aaagagcaac taaatccagc ttcaaaggga ggtgagtagt atgtttagtt ttagcgatgt
    55201 gaaaatgatg tatgattggg gctgtttcac taacgaacaa gtaatggttt tcgttccgtt
    55261 gtgcattact gaagaa
//
LOCUS       DAZUJO010000010        55006 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000010 DAZUJO010000000
VERSION     DAZUJO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.010"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..268
                     /locus_tag="ACG8OL_000677"
     CDS             95..268
                     /locus_tag="ACG8OL_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816685.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(334..942)
                     /locus_tag="ACG8OL_000678"
     CDS             complement(334..942)
                     /locus_tag="ACG8OL_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4816686.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACG8OL_000679"
     CDS             complement(948..1976)
                     /gene="vanB"
                     /locus_tag="ACG8OL_000679"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4816687.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            complement(1969..2940)
                     /locus_tag="ACG8OL_000680"
     CDS             complement(1969..2940)
                     /locus_tag="ACG8OL_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4816688.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACG8OL_000681"
     CDS             complement(2937..3764)
                     /gene="vanW-B"
                     /locus_tag="ACG8OL_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS4816689.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACG8OL_000682"
     CDS             complement(3782..4588)
                     /gene="vanY"
                     /locus_tag="ACG8OL_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS4816690.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACG8OL_000683"
     CDS             complement(4759..6102)
                     /gene="vanS-B"
                     /locus_tag="ACG8OL_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4816691.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACG8OL_000684"
     CDS             complement(6105..6764)
                     /gene="vanR-B"
                     /locus_tag="ACG8OL_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS4816692.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(7382..7549)
                     /locus_tag="ACG8OL_000685"
     CDS             complement(7382..7549)
                     /locus_tag="ACG8OL_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816693.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(7707..7892)
                     /locus_tag="ACG8OL_000686"
     CDS             complement(7707..7892)
                     /locus_tag="ACG8OL_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4816694.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            8037..8408
                     /locus_tag="ACG8OL_000687"
     CDS             8037..8408
                     /locus_tag="ACG8OL_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4816695.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            8666..8995
                     /gene="mobC"
                     /locus_tag="ACG8OL_000688"
     CDS             8666..8995
                     /gene="mobC"
                     /locus_tag="ACG8OL_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4816696.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            8956..10284
                     /locus_tag="ACG8OL_000689"
     CDS             8956..10284
                     /locus_tag="ACG8OL_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4816697.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            10281..10652
                     /locus_tag="ACG8OL_000690"
     CDS             10281..10652
                     /locus_tag="ACG8OL_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4816698.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            10835..11071
                     /locus_tag="ACG8OL_000691"
     CDS             10835..11071
                     /locus_tag="ACG8OL_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816699.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(11371..11574)
                     /locus_tag="ACG8OL_000692"
     CDS             complement(11371..11574)
                     /locus_tag="ACG8OL_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFS4816700.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMLVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(11578..12522)
                     /locus_tag="ACG8OL_000693"
     CDS             complement(11578..12522)
                     /locus_tag="ACG8OL_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816701.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(12523..16443)
                     /locus_tag="ACG8OL_000694"
     CDS             complement(12523..16443)
                     /locus_tag="ACG8OL_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS4816702.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(16592..18673)
                     /locus_tag="ACG8OL_000695"
     CDS             complement(16592..18673)
                     /locus_tag="ACG8OL_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS4816703.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(18670..19899)
                     /locus_tag="ACG8OL_000696"
     CDS             complement(18670..19899)
                     /locus_tag="ACG8OL_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFS4816704.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(19889..20140)
                     /locus_tag="ACG8OL_000697"
     CDS             complement(19889..20140)
                     /locus_tag="ACG8OL_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFS4816705.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(20163..22154)
                     /locus_tag="ACG8OL_000698"
     CDS             complement(20163..22154)
                     /locus_tag="ACG8OL_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS4816706.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(22186..24585)
                     /locus_tag="ACG8OL_000699"
     CDS             complement(22186..24585)
                     /locus_tag="ACG8OL_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFS4816707.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(24515..24937)
                     /locus_tag="ACG8OL_000700"
     CDS             complement(24515..24937)
                     /locus_tag="ACG8OL_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFS4816708.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(24951..25487)
                     /locus_tag="ACG8OL_000701"
     CDS             complement(24951..25487)
                     /locus_tag="ACG8OL_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFS4816709.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(25524..26387)
                     /locus_tag="ACG8OL_000702"
     CDS             complement(25524..26387)
                     /locus_tag="ACG8OL_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFS4816710.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(26479..26694)
                     /locus_tag="ACG8OL_000703"
     CDS             complement(26479..26694)
                     /locus_tag="ACG8OL_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFS4816711.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(26771..28579)
                     /locus_tag="ACG8OL_000704"
     CDS             complement(26771..28579)
                     /locus_tag="ACG8OL_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS4816712.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(28576..29052)
                     /locus_tag="ACG8OL_000705"
     CDS             complement(28576..29052)
                     /locus_tag="ACG8OL_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFS4816713.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(29127..29339)
                     /locus_tag="ACG8OL_000706"
     CDS             complement(29127..29339)
                     /locus_tag="ACG8OL_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4816714.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(29361..30326)
                     /locus_tag="ACG8OL_000707"
     CDS             complement(29361..30326)
                     /locus_tag="ACG8OL_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFS4816715.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            complement(30817..31338)
                     /locus_tag="ACG8OL_000708"
                     /pseudo
     CDS             complement(30817..31338)
                     /locus_tag="ACG8OL_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(31354..32052)
                     /locus_tag="ACG8OL_000709"
     CDS             complement(31354..32052)
                     /locus_tag="ACG8OL_000709"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4816716.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(32009..33670)
                     /locus_tag="ACG8OL_000710"
     CDS             complement(32009..33670)
                     /locus_tag="ACG8OL_000710"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS4816717.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            33888..35288
                     /locus_tag="ACG8OL_000711"
     CDS             33888..35288
                     /locus_tag="ACG8OL_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS4816718.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(35540..36619)
                     /locus_tag="ACG8OL_000712"
     CDS             complement(35540..36619)
                     /locus_tag="ACG8OL_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4816719.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(36705..37664)
                     /locus_tag="ACG8OL_000713"
     CDS             complement(36705..37664)
                     /locus_tag="ACG8OL_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4816720.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(37676..39187)
                     /locus_tag="ACG8OL_000714"
     CDS             complement(37676..39187)
                     /locus_tag="ACG8OL_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS4816721.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(39209..39772)
                     /locus_tag="ACG8OL_000715"
     CDS             complement(39209..39772)
                     /locus_tag="ACG8OL_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4816722.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(39778..40641)
                     /locus_tag="ACG8OL_000716"
     CDS             complement(39778..40641)
                     /locus_tag="ACG8OL_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4816723.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(40655..41551)
                     /locus_tag="ACG8OL_000717"
     CDS             complement(40655..41551)
                     /locus_tag="ACG8OL_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4816724.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(41726..43015)
                     /locus_tag="ACG8OL_000718"
     CDS             complement(41726..43015)
                     /locus_tag="ACG8OL_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4816725.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(42987..44504)
                     /locus_tag="ACG8OL_000719"
     CDS             complement(42987..44504)
                     /locus_tag="ACG8OL_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS4816726.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            44702..45592
                     /locus_tag="ACG8OL_000720"
     CDS             44702..45592
                     /locus_tag="ACG8OL_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS4816727.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACG8OL_000721"
     CDS             complement(45665..46489)
                     /gene="nadE"
                     /locus_tag="ACG8OL_000721"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS4816728.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(46501..47973)
                     /locus_tag="ACG8OL_000722"
     CDS             complement(46501..47973)
                     /locus_tag="ACG8OL_000722"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS4816729.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(48610..48972)
                     /locus_tag="ACG8OL_000723"
     CDS             complement(48610..48972)
                     /locus_tag="ACG8OL_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4816730.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            49195..49947
                     /locus_tag="ACG8OL_000724"
     CDS             49195..49947
                     /locus_tag="ACG8OL_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4816731.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(50087..51010)
                     /locus_tag="ACG8OL_000725"
     CDS             complement(50087..51010)
                     /locus_tag="ACG8OL_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4816732.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(51025..51297)
                     /locus_tag="ACG8OL_000726"
     CDS             complement(51025..51297)
                     /locus_tag="ACG8OL_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816733.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            51974..52204
                     /locus_tag="ACG8OL_000727"
     CDS             51974..52204
                     /locus_tag="ACG8OL_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816734.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            52210..52509
                     /locus_tag="ACG8OL_000728"
     CDS             52210..52509
                     /locus_tag="ACG8OL_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4816735.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(52545..52916)
                     /locus_tag="ACG8OL_000729"
     CDS             complement(52545..52916)
                     /locus_tag="ACG8OL_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816736.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(52918..53319)
                     /locus_tag="ACG8OL_000730"
     CDS             complement(52918..53319)
                     /locus_tag="ACG8OL_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816737.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(53333..53740)
                     /locus_tag="ACG8OL_000731"
     CDS             complement(53333..53740)
                     /locus_tag="ACG8OL_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816738.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            complement(54044..>55006)
                     /locus_tag="ACG8OL_000732"
     CDS             complement(54044..>55006)
                     /locus_tag="ACG8OL_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4816739.1"
                     /translation="PINTFAYTINNEFNLGPNEGSSQVANNQYILLHETANETATGRN
                     EAQYMKRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIYRPDVNQAYNTLGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT        13925 a        13338 c        11978 g        15765 t
ORIGIN      
        1 gaggggcaag gctttttttg agtggtggaa gtggattaga gcatggcaat agcaaaacag
       61 catagcggca tcctcctatg ggccgccaca ccttttgctg ccagaaaaga acggcggcct
      121 tgatttctca aaaccgccgc cagcaaaata caaaatgggc gtgggaaagg tctgcccaac
      181 agcggttttt ttcttttctg ccgctgggca gattgaatac tataatttgc aatgtattac
      241 aaaagatgct ttaaccactt tccagtaaaa gattgtccat gaccagcata gattttatca
      301 aatccaaaat ctgatacaaa accaatactt tcattatgaa acggcaaaat taaaataggt
      361 atcggggtat ggctcatcaa tcaacttata gtgccaccat tcaaagcgat aagactgaaa
      421 tccgctgctt tccatgattt tacgcaagca tcttcgattt tgtgcctctg catcccctat
      481 cccttttgct gtatgatgcg accgttcgtc cataaaatcg aagttacttc ccattgaaac
      541 aagttcccct gtatccaggt ggtagagcgt aagatcaatt gcgcttccac ggctatggct
      601 ggattgtgag gccacataac cctttgtaat caactcggct cgctctatat tggggtaaaa
      661 tttttctttt gtgagattgt cctccggttg cgctgcccaa cgcaggaagc agtccaccgc
      721 agttcttggc cgatatccat cccataaaag caagccatag ccaagcgcag ccgcatggat
      781 ttgtgcctcg cgcagagcaa acgccacggc ctttgtgccg atgatgcgat tcacctcata
      841 cccatccacc ggttttcctg tgaagttatc ccatgtggcg tacttggcat cccaacggac
      901 gccatgcaac atttcatcta aaaacaaaaa accattttcc atacttatca cctctttaac
      961 gccaatgtaa tcaggctgtc aatcagtgca ggaagcgtga ttcctgcggc ggccatcata
     1021 cgtgggtagc ggctgtacga tgtaaaaccg ggcagggtat tgacctcatt tagaacgatg
     1081 ccgccatcct cctgcaaaaa aagatcaaca cgggcaagcc ctctgcatcc aagcacccga
     1141 tatactttct ttgccgtttc ctgcacccga tttcgttcct cgaccggaat gtctgcggga
     1201 actgtaatca tcgcattttc tgagcctttt tccggctcgt tttcctgatg gatgcggaag
     1261 gtaccgtggc tcagccggat ttgatccact tcgccgacaa tcaaatcatc ctcgttcccc
     1321 atgaccgcac acccgacctc acagcccgaa atcgcttgct caattaagat ttttccatca
     1381 tattgtcctg ccgcttctat cgcagcgtta agttcttccg taccgtttac tttggttacg
     1441 ccaaaggacg aacctgaccg tgccggcttc acaaagacag ggtaggtaag cgcacccgcc
     1501 tccggcttgt cacctttatc aatcatttga aattcgggaa cggcgatgcc cgcatttttt
     1561 gtaagaatgt aggccagtga tttgtccatg caagctgcgg agctttgaat atcacagccc
     1621 acatagggga taccagacaa tacaaacagc ccctgtatcg caccatcctc cccgcatttg
     1681 ccatgcaaaa ccgggaaagc cacatcaata cgccgtgttt cgtattcgct ttctttcatg
     1741 acaagcagcc catgcgtttt cctatccggg gagagtatgg cggggagact gtcggcttcc
     1801 cattccgtac atggcttctt gcatagcttc catacaccgt tttttgtaat tccgatgtag
     1861 tgcggatcga atttttccgt atcaatgttc gcagcaattt ctattgcgga ttttaccgac
     1921 acatcatgtt cctccgagca accgccgaag atgattgcga cttttattct attcatgctg
     1981 taaactcctt tcaaaattga gacaattcct gattgttttt tctgtagtat cctgcagcac
     2041 acgctcagtg tagtacgccg tatggggtgt gatgatcaca ttcggcatcc ttaaaagctg
     2101 cgacaaaaag ggatggtcaa gcactttctg cgagcaatcg gtataaacaa actgatcctc
     2161 gccctccaac acatccagtg ccgcaccacc cagctttccg ctttccaacg cctccaccag
     2221 cgccccggta tcgacaagcg ctccgcgccc ggtgttgatt aaaaatgcgc cttgcttcat
     2281 ctctccaatt tgcttctgac cgatcagatg gcgggtatcc gcacaaagcg gcacatggag
     2341 cgtaacaata tcgctgtttt ttagaagctc atcaagctgg acataatctg catccatttt
     2401 tcggctgtta tcataggcca gcacatggca tccaaatccc cgcagccttt tgacgaccgc
     2461 ttgcccgata tggccggttc caataactcc cacagtcata tcccccagtt ctttcccccg
     2521 gatacgatcc aatctgaaat cttgttgcgc cacggcgtgc atggtgggtt ttgcaccccg
     2581 tatggccatc agcatcagca tcaaagcata atccgcaacg ctgtccggcg aatacgcaac
     2641 cgtgccaacc gagattccca ttctctcggc ggcagtcgta tcaatgtgat cgcagccgat
     2701 gctgcgggta gaaatgtatt ttaccccgac ctttctcagc gcaagaattg tcgcctcgga
     2761 aacctcggac ttatggccta cgctaacgca ttgattgcca gcggccaatt ttgcgttgtc
     2821 tgccgatatc gcatcgctaa tcagcgtagg gataatatga aaatccggcg ataaagcgcg
     2881 gaaagcattt gcctcatcct gttcgcatcc aaaaacagta atgcccacac tttttctcat
     2941 tggttctcct cctgaatatc cacactttcc gggaggggat aacagatttt gcatttgttt
     3001 gtataaagca gcttgcaatc tattatttcc ccggtaccta aggcaacttg catccgttta
     3061 acctgggcat attcataaat cccgccactt tcacggacat actggatact gccatttgta
     3121 attttgtata atgcttgggg ctctttgtcg gcggacacct gaccgatgat tttctcatca
     3181 tctaaggtca cccatatttg gtaggtgcag tcggtatcgt ttcgcacttt taaatcaatc
     3241 cagccctctg aaatggttgc atccacccct ttaatctcgt cactgtttgg ctctggaaac
     3301 tcctttactt cgtgacctct gcgctggata attgtcaatg gcgtatgcag gaacatccaa
     3361 aatagtaaat tgctcatctg gcacataccg ccgcccgaca tggtggtgag tttaccattg
     3421 gtcaccgtaa ggccgtcttt atagggggta tctttgtccg catggcgtac cagccaccag
     3481 aaagaaaagg gttcccccgg tcttatcaat aagccgttta aggtctttgc tgccagcttt
     3541 aagttgaaca ccttgttttc ctggtatatc atgtcaaatc cggtattgtg gttgtataat
     3601 gcgcaatccg tttcaaagag caaatgggaa agcgattttt cccctatcgt ctgcgcatag
     3661 cgacagccgt caaatctcat tcccgcataa aagcacattt ttctctgcgc ccgtctcatt
     3721 ggaagcagga acgggaaacg ctgtgtcaat ctttttctgt ccataacata acatcctttt
     3781 ctcatttttc tggttttaaa tattcctcaa ggcaaacccc ttggttgtac atctctgtgg
     3841 cagcttcgat gccaacatat cggtaatgcc acggctcgtt gctcacaccg gttatctctg
     3901 tcttgtctgg tgggtagcga cgaataaaac caaaacgata gctgttttca tccagccatc
     3961 tataaacctc gttgcctgtt gaatgaattc cgtccgcatt gatatccaca gcaagaccaa
     4021 gctgatgctc gctcgttccc ggcacggcca cccaagtttc tgcttccgct ttagcctgtg
     4081 cagaggtgta gcctttcgcc ttatattcgg caattttttc atccataatt tcttgctgtt
     4141 tttctgttgt tcggtatccg gatgcgacaa tcgggtaaac tccatcagtt cttgcggcat
     4201 caaacaaatc ttgaagatag ggagaaatcc gaatatctat ccgctcacca tttgatagtt
     4261 gctcaagctc cacatcgtac tgtgcgggga tggggttctg cctgttcact aaaatcaggc
     4321 tccattcctg ttcatctccc tgttctttgg cggtggcaag agcgttttcc tccaaattct
     4381 ccactacgtc agtgctgctg cctgtatgag ttgcagggat gggcggtatc tgtgttgcct
     4441 ccaatacgga agccagtctc cgccccaaaa acagcgtgca gactgtgaac gaaataatga
     4501 acgcacatag aaaagcccgc ttttccacgg attgtttcat atgccgtttg tgatgatccg
     4561 cattggaatg atagttgctt ttttccattt gatcgaatgc ctcctttgca ataaaactac
     4621 gatttgtggc tggtacgatt gttattatgg caagcgcatc tgtactttcc ctgtagccag
     4681 tctgtagttt tcctgtagca gcaaaaaaag accggcaggg ttatacctgt cggtcaaaat
     4741 catttaagtt ttaaatattt acaatgttga tgtgagcggt aaatccagcc agaacaaaac
     4801 gccatccgag gtgttttcca gcgcatactg gaggctcatg gcatccagcg ttttttgtac
     4861 gatggcaagt cctaaaccgc ttcgcccact ttttctgctt tgcgcctgat caatgcgata
     4921 gaatggggtg aacagccttg gaagcgcagt atcatcaatg tgaacgccca tgttcaaaac
     4981 aaaaaggcgg catttttcag caccaggctc actccatatc cgtacctcgc ctccctgcgg
     5041 cgtgttctga actgcattca atatgacatt ggatagtgcc ttttggagca gcctcggatc
     5101 ggacaggaca atttgcccgg ctggaatatc tgtgacgaac cgctggttgt ttgcctctgc
     5161 caaggtttga aaatcgggca gcaactcggc aaccgtgcgc cctatgtcca acggttcagc
     5221 tatgggtacg attctcccat cattcaggct gacaagctcc agtatttcgg aaatgatttt
     5281 gccctgccta tccatcattt tgatgcattc gcgcagatac ttagaatgat ctttgtagtc
     5341 accgatattt tcaagcattc cctccaacag aacgcttgta gccgcgatgg gcgtttttag
     5401 ctcatgagaa gctgccgcaa agaaatatcg ctgtgtttcc tccaactcat gttcccttgc
     5461 gatttcatcc tccagccttg cgatggtttc tttcagcctg acatacatgg aatgcatatc
     5521 atgagccagt gcgccaagct catcctttcg ctccagcggc ggcggtacat ctttcaggtt
     5581 tgccatttta ttcgcactgt ccgctaaggc tttgatcggc gttgtcattt gccgcgcaaa
     5641 gatatacgcg cataaaaggc tgaatacaac cattatcgca atcataacaa ttccccgaat
     5701 tgtcagccct tgataaagca atcccacacc tgccttgctt tgagcaacaa tcgaaatatt
     5761 atcatctcta tgtaccacat aaagaaagtc gggtctaaaa ctatttgatg tattggcatt
     5821 tggtgtggca tagagtacgc ttccctcttt atcttcaata taaaactcaa aggattggtt
     5881 attgtagtga aacagccctg ccacctcttg aatatcaagc ctatcgctat tctgaatcag
     5941 ttccaccaat ggctgatagg attttactgt ttgctgtaat tccatcactc tgaaataaga
     6001 cacaaattgc tgtgcaaaca gtgttgctgt tacaccgaca agcagtagca gggcaatgat
     6061 cgtataggaa aaaaccttaa tgaaaatccc ttttctttcc attattatga ttcctccaat
     6121 cggtatccta caccgcgaat ggttttgatg atattttccg gcagcttggc ccgcaaattt
     6181 ttgatatgag tgtggactgt gctgccatca ccctcaaagt catatcccca tatacgtgac
     6241 aaaatgattt catgcgttaa ggttctgcct ttgttctgca ccagcagcaa aaggatttca
     6301 aattcttttc gtgtcagggg cagctctgta ccgtcacaaa gtaccgtgaa atcctccggc
     6361 agaagtgtca gcctgccgac acggatttcc tttgccagcg caccgctgcg ccgcagcagg
     6421 gcttccaccc gctttagtaa aatctgcatc ttgaatggct ttgttacata gtcgtctgcc
     6481 tctgcatcaa acgcccggat ttggttttcg tcatccgaca gggctgtcat catcagaatg
     6541 ggagtatcat ttttcgcacg aaattcacgc aaaagttcat gcccgttcat accgggcagc
     6601 ataatatcaa gaataaccag ttgataagtg ttttcgtaaa acttggtgta tgcctcattt
     6661 ccatctgtgc aggcatccac ctgatatcct gcctcagcca gaaacgccct tactgtatta
     6721 cagatatgat catcatcctc gacaagtaga attcgtatcg acataaaaat ccctccattt
     6781 taagaagata acataacagt ctgtagtttc cctgtagttt caaagcgatt tcttttcgga
     6841 ttttaatcat ccgtcattgg gatactcgct tcttttcatg gtgtatgtat ctatattatt
     6901 ggtttaaacg aattaaatca agataataat aattaacacc ttcttaatgt ttgggatgtt
     6961 tcgattacag cggttgtcaa aacatcccga taatactggc aagcaatgga tcagaatatc
     7021 ctttacggat attctgatcc accacttgcc cgcctgcggc gttctcgttt tgcttgttgg
     7081 ggacacccca agcctcagca aaatgcgcgg cctctttttt gcgaatgcac cttgcggtat
     7141 ggcttttcac acaggcaggg cctgtgtgta ctccctccgg tcgctcttgc cgcactccgc
     7201 gcagcagatt cagtcctaat accgttcaag acagtagaaa atcagcaagt ctatattcct
     7261 catatcttca tcatccgtgt ccttgcagtt acaggaaatg taataatcgc caaatgtaaa
     7321 tcccagaata ttttcctcaa aaagccgtac ctccccggta ttaaaatcaa tcaccccata
     7381 ctcaaaggcg cattagacca gcagttatcc aatgtctgga gacttcacca taaagaatcc
     7441 agatgccggc cttttcaatt ctaccatata cggctgatac agcagcagct tcctcgtctt
     7501 tgtttcgggc attttccatt tctgtcatga taaagtttcg cttcttcata catccatgtg
     7561 taaatctttt ctttctgttt cagatgagaa tactttttat cggtctggag cagcctgcca
     7621 tctatcatct tatagttttt ccgtgacaaa ataccatgct ttctctctct tcaaaaccat
     7681 atccaaaatg cgccggtttc attatactat tcagtaaccg cctttccttt ctttgtacgc
     7741 ctgtcaatcg gcttttccgc agttttggga atgagccaga tcgtcgccat ctttacagcg
     7801 ccggggatgc gtcccgcagc acaatagtag tttatccatc gtgggggcac accccatttc
     7861 tcgcctgcct ctttcaacgt catgtagtcc atagtacacc tccacagggt acattataat
     7921 tcttttgctc gaagaataaa agaaacaaaa tgtgaaatat aaactttaag aaaggtgcat
     7981 ataccaacat tccacgggca gagtatgaaa tatacaatgt aagtgggagg tgcgatatgg
     8041 cgaatattga agattgtccc ggctttgaaa ctttcggcgc ggatgtgaaa gcggcccggc
     8101 aagcaaagcg cgtttcgcgc aaagcaatgg cagagaaaat aaatattgac tggcgctatc
     8161 ttgccaacat cgaaaacgac ggggccattc ccagtctgcc ggttgtcata cagcttatta
     8221 aagcctgcgg gcttcctgtg gaacgctact ttaacccgga gatcatgcgg gaggaaagcg
     8281 aactgcggca aagggtcagc cacaagctga aactctgccc cgaagaatac ctgccgatca
     8341 tcgagggcgc gattgacagc gccatactaa ttgaaaagcc gaagaagaat gaaacggagg
     8401 atgtgtaact ggcatcctct gttttacttt tagcgggagc tgcacagcgt agcttctatt
     8461 tttttgcact tttttctttc cagagggcta ttttgcgggt agtttggggc tttggaaacg
     8521 tcttgttagt ggagaaacag taacgtagca agcataggga gtgtagccac tcccggaaaa
     8581 acggcctgcc ccgtccctgc cgggccgcgt ccgttttgct tgttggggaa atccccaaac
     8641 ccctgaatgg aaaggacggt gaagcatggc aaacagaaag cgaaaaatcg tgctgcgcgt
     8701 ccctgtgacg ccggaggaac gggctatgat tgaacagaaa atggcgctgc tgcatacgaa
     8761 gaacttttca gcctacgccc ggaagatgct cattgacggc tatatcgtca acatggacac
     8821 cagcgacatc cgggcgcaga ccgccgagat acagaagatc ggcgtcaacg tcaatcagat
     8881 tgcaaagagg ctgaacggta tggggccggt gtacgcacag gacatcgagg atataaaggg
     8941 ggcgctggcg cagatatggc agttacaaag atacatccta tcaagtcaac gctgaaaaaa
     9001 gcccttgact acatcgagaa cccggacaaa actgacggca agctgctggt ggcctccttt
     9061 gcctgctctt atgaaacagc ggacattgaa tttgaaatgc tgctggcgca ggcataccaa
     9121 aagggcaata atctgggcca ccacctgata caatcctttg cccccggcga agccacgccg
     9181 gaacaggccc atgagatcgg gaagcagctt gccgaggaag tgctgcaagg gaaatatccc
     9241 tatgtgctga ctacccatat tgataagggc cacgtccaca atcacattat tttctgtgcc
     9301 gtggatatgg taaatcagcg caagtacatt tccaacaaac agagctatgc ctatatccgg
     9361 cgcaccagcg acaggctgtg caaggaaaac ggattgtccg tggtgaagcc cggccagagt
     9421 aagggcaaga gctatgccga gtgggacgcc cagcggaaag ggacgagctg gaaagcgaag
     9481 ctcaaagcag ccattgacgc cgctatcccg caggcaaaag attttgatga tttcctgcgg
     9541 ctcatgcagg cgcaaggcta tgagatcaag ccgggaaagt ttatttcttt ccgcgctccc
     9601 ggacaggaac gctttacccg ctgcaagaca ttgggggaag cctacaccga ggaagccatc
     9661 aaagagcgta tcaaaggccg cgttatcacc agagcgccca aagagcgcaa ggggatttct
     9721 ttgcggattg atctggaaaa caacattaag gcccagcagt cggcaggcta tgagaggtgg
     9781 gcgaagctcc acaatttgaa gcaggccgca aagactttga atttcctgac tgaacatggg
     9841 atagacacct acccggattt ggaaagcaaa gtggccgaga tcaccgccgc cagcgacgag
     9901 gccgccgcta ccctcaaagc tatggaacac cggcttgcgg atatggcggt gctgataaag
     9961 aatatttcta cctacaagca gctctggccc gtggctatgg aataccgcaa tgcagcggat
    10021 aaggcaaaat tccggcgtga gcatgaaagc acgttgattt tgtatgaagc tgcggcaaag
    10081 gccctaaagg gacagggtgt gaaaaagctc cccgatctgt acgccctgaa agccgagtac
    10141 aagcgattgg ccgaggaaaa ggaacggctg tatgagaagt acggcgaagc gaaaaagcag
    10201 atgcaggaat acggcatcat caagcagaat gtggacggca ttttgcgtat gacgccggga
    10261 aaggaatgga cgcaagagct atgagtattc cacgaatgaa ttaccctcaa tatcgcaagg
    10321 cccggaagct gacgcatgag tgctgcaact attgtgacgg caactgcctg ttgctggacg
    10381 acggcgaaga atgtatttgc gtacagagca tttcctattc gcttctgtgc cgctggttta
    10441 aggccgctgt cctcccgctg gatgcggccc tgtatgccga gatcaccaaa gaccgggacg
    10501 aagtgaaacg ctgctgtgag tgcggggcca cgttcacgcc gaagtctaac cgggccaaat
    10561 actgcgcggc ctgctccaaa cggatgcgcc gccgcaaaga ggccgagcgc cagcggaaaa
    10621 gatactggaa acctacgcac ttagagctgt aaaagccctt atatttcaag gcttttccgg
    10681 catggtcagc ggggtatgtg ggtgttttat cctatccccg ccgaagtgct gttctaaatg
    10741 cgtagaattt accagcagaa aagcgccgtt ttctctggtt ccatgcttcc gaatatggta
    10801 tcatagaaaa aatcagtctg acgggggtat caggatggac ggacaagaaa agctgctgga
    10861 ctatgaaacg atcaaagccg ccgttgccgg tgagaggtgg gccacggaaa aagtgttggc
    10921 ccattatgct gactacatca acgagctttc cacggtggag atcagacagg caaacggcaa
    10981 aaagaagaaa gtcattgacg aagatatacg gcagcgcatt tccctgaaac tgctggaagc
    11041 attgccgagc ttcccgctgg aacagagata gacggcaaga gggctgcacc ctcttacgcc
    11101 gctttgcggc tatcacctgc acacccctga aacagataca cccgctgcgc ggctatatct
    11161 gtttcagggg tctaattata gctgcaacct acgcatttaa gggattgaaa accgcccgct
    11221 gcaaggcttc tcccgcatgt ttgcaggggc aagcgaatac ttcatcctat ccccgacaat
    11281 accgggttct aaatgcgtag gaataggcaa aaagaaaaga cgccggacgc aagcgaccac
    11341 atggccgccg cgcccggcgt ctgctgttcg ttattcgtct gtgaagtgga acttctggcc
    11401 gtcatagtcg gcatccgtca tgcgttccag cttggaaagg gtctgctggg cctgcgcccg
    11461 catatcccca tccataacca gcatgacggc ggtgagcctg tttatcgttc tgcgccggtc
    11521 tgcattgtgg tacatacaga tcaggttttc ttcctccacg gtaaagtgca tcattcctta
    11581 tacctccaat tcattctttt tggttctggc cgccgccttt tttgggctgg cggcctgttt
    11641 gctgtcggat agctgcttgc ggatagaggg ccggggcctg cgaatactca catcccggcg
    11701 ctcgtcccgg ttaatcacgg catacacgcc atgcctgcct ttcatcccgg taaaacacag
    11761 ggatttatag ggcagcatgg caaagagccg gtcattatcc ttggtggaag caatcgccgc
    11821 aaagtgcggg gagatctcca ccatgaaatg cgttttattg gggctgttag gcgcggacag
    11881 atctttcagg ctgtccacaa tacgccgggc ctcggtttca atccggctgt catgcaaagc
    11941 ggtgacgtgg actttgaaat cgccggggac ggcccgcccc cggctgtgct gctccgtccg
    12001 tagcgcctcc tgcagcacag ccgggtcatg gggctgggcc tcatagtaca aaaagtttcc
    12061 caatacccgg tcagggcttc cgtcaaaacg ctggctctcc gggatgtcac gggtgccatg
    12121 ctcataaaag agccggttca cactcaccgg ctgggcggtt tcaatgacgt gccggaaatg
    12181 ctgcggatag tccagctcat agagattgcc ccggatagtg ccgccctgtg tgccggtcag
    12241 ctccaccgcg taggccagta tcttgtcacg ggtctgctcc ccgtaaaatt tccatgtgtt
    12301 gtactgtcgg gtgtctttca ggaaaacatc acgttcccgg aaacagtacg tcccggaggg
    12361 ccgggacatc cagagcaggg tcttgtctgc ggcctgatcg ctggcggcat cccgcctgat
    12421 aatgtctttg tcaatgccaa agtcactttg ataaaacgcg gtgttctgcc gcatgatctg
    12481 ttccagcgcg gccagaacgt ccacattttc aaacttctgc atttactcta cctccaatcc
    12541 ctttgatttt tcccgcgccg gtggctgctt ggcctgttcg gtcttggccg cgttgagctg
    12601 cgcccggata gagggctttt ctttctccct gcgggccgca agctgtaagg cgctgggctt
    12661 ttcgccggaa aggggctgga tgcacttgat acggtcagcg acgcggattg tggtgtcgtt
    12721 taactgccgc tgccatgccc cggccttggg cgaccagcgg aaaccgttgc ttttcagctc
    12781 gtcgcgtaca gcagcatccg gcttcccctc aaagaagatt tgcaggcggt tggccacccg
    12841 gttgatctcc acagtgccgc cgtcaaactc ccatcccaca taggccgcct ccttttggcg
    12901 ggtcaattcc tcaatgcggc cctttaaccg gcgtatctcg gcgttattgt tggagagctg
    12961 ccaggactgg aacggttttt tctcatagtg ccagcccgac gccatatccg ctttcaggtt
    13021 ttcaatgcct ctttcggtca gatgggggca tccgtccagc gtcccatgct tgcggtagta
    13081 ggcattgacg gccttcatca actcctgcga cgcttccagc ttttccagtt tcttttccag
    13141 cttggaaacg gcctgcggat cgtcggcgct gataccaccc atgccggtgc tgcggatttt
    13201 gtcaagcaag ccctgaatgt actgaaattc ctccatgttg cggtcactgg cggcgttctg
    13261 cttttctttc gcccgtaccg ggaaattgga cggcccggca atcaggacag aggggacgcg
    13321 ggcggtaatc tcatagccct tattcatgtt ctcggccagc ttgcgggcgt aggtgtcaag
    13381 gaggctgtct atcttttcgt gatactctgg ctccacacgc tttttctggc gctccgcaat
    13441 ttccacggcc tgatcgacat agtgccggta ttccgccgtg gcgctgccgg gccggtagtc
    13501 agaaaagctc atggcttcct tggcgcggcg ggcggctccc tcattgatgg gataatagcg
    13561 gacagccgtt tgcgggggcg cagcctccgg gggttcctcc tgtgtctggc cggaagcaac
    13621 tgccacatcc tcaaaagata gctgttcggg ttcctccggc tgcggttcgg gcggcgtatt
    13681 gttgataatg ccgtcaatca tgttttcgtt ctgctccgta gacagttcgg cggtacgcag
    13741 gggattttcc cgcaaaaact ccggcacttc catatagccg aagctgtcta catagtgggc
    13801 ggtgtcctgt ccattctggt gcagcaccac cacgtcggaa acggaaaggc tgtgtcccct
    13861 aaaatctgcc gggtggtcga tgttgaatgt gcggtaaata tcctccaaag acgtgctttt
    13921 atccagcggt gccgtataaa caaggtcata gttggcactg tccacggcca gccctgcggc
    13981 ctgtaaacgc tcgtatggct caaaccgata gtcgcgggtt tcctccccgc ctcgaagctg
    14041 gtagatggaa aaggtgtcct gttggggtgc ttcatagcca gccgccgcgt attcctccac
    14101 ggtcataccg gctgcggcgg cctcctgcgc gatctcggcc tgtacctgtg ctttgtagtc
    14161 ctgcaagctc tggtcgaagt cagccagttt cggcggctcc ggcaactgat actggccctg
    14221 attgagcatg gggcggcact cctgcacata agagagaagc gcccccaaat aggccgtttc
    14281 ctccggcgtc agggtttccc cggactgtaa gcggccctgc gccgtctgtt ccatttgggc
    14341 aaggctgcaa tgcagcttca tataggggac aaactcgcct aacagcatat cccgatgggc
    14401 cttgtcctgc tcccagccct ctatgccggt tgtagcgatt acatgctttt tccagttttc
    14461 gtcctgcgcg taaaattggt ggtagctctg gatatgttcg atcagggaac cgtccctatc
    14521 tccaaaatcc tgccgcccct cgtagctgtc cggctcgccc tgaaagatac agtcaatgcg
    14581 gaacgcggtt ttgtcatagc ctgcctctgc cggatatgct gcatccagac gggcaaacag
    14641 cgcgtcggcc tcatgcaacg gcatttgctg gccgtccttt agctgggggc tttcgctcca
    14701 tagcacagtc acaagcggct cggctgccgg atcaagggcc ggggcctgtt cttcctcggc
    14761 ctgcgccggg gccgctgcct ccggggtaac gtcaaccgtg acggcctcct gttcctgccg
    14821 ggcctcgcgc tcctgctgaa gctgggcaaa atggccgtcg atctcggtta tcaattcgct
    14881 ggccgtggcc cggatggttt caagggaggc tttcagctcg gataactccc tgccgctgct
    14941 ccaatcggcc acatagccaa aagaatagtc cgacgtgtcc agcccgtaat actggcagac
    15001 ggtataggcc acgctttcgg cctgtacctc gcgggtgctg cggtctggcc gggccgcctg
    15061 ttcctcggcg ggggcctccc ggtcaatggc gtggagcttg gcatgggcaa tctcatggat
    15121 ggcggtcttt agggtctgca attcgctcat gccctcgttg atggcaatgc gctgttttgc
    15181 gtagaagcag cggcccttgg ccccgtctga aatcggctca atgcttatgg gatagggtga
    15241 tgtccgttcc agcgcggcaa agaaatcctg atactgctgc acgtcgccgg taagttcact
    15301 gatatagggc atgggaaaat ccttgccctc ggtctggctg atgtcaaaga ctgtcaccac
    15361 cttaaaggcc ggaaccgtca cttcctgttc ttcggtcacg ggcttgccgt ccgcgccgat
    15421 cacgggctgc tgggtggccg ggtcgatttt ctccatctgc tgcttaacct tgaacggggc
    15481 cggggccagt atcttaatgc ctttctcgcc ccgcttcaca tggcggtcaa attcatcctg
    15541 ccacctgtta tagcccgcca caatcgaacc gccctgcatg gcgatcagca gcgtattgtt
    15601 cagactgtaa tcgtgcatct tggacatagc ggataagtaa gccatatagc ggctactttc
    15661 aaaaagctcc tgtacgccgg tttccagacg gtcagtaatc actttcatgc gttctttgct
    15721 gttcatgccg tccagcatga tcgggataac cgggggcggc gctttgtcgg ggctttcgga
    15781 tgcctccggc tgctgggcct gcggttcctc ccggtcagct tccggctggg ggtagtgcat
    15841 cacccggtat tcctccggca ccggctggcc ctcatggacg cgctgccatt cgtcgccgct
    15901 tctcaccaga tagccgtact gggtaaaaac gccttgttcc tgctgggcaa tgtggcgtcc
    15961 aaactgcgcc gtatcaatcc cggctttcca ttcctccggc atatccacca tgccggaacg
    16021 gttcaggtag tagtcgccca actgtccatc ccccctaacg tcgggaagat gcacatagta
    16081 atccacattt tcggtgtagt cgatgatctg gccgatattc tcaaagccgc cacggggatt
    16141 ttgcagggcg gcgttcagct ctgcgatctc ggcggcgtca agctggccca gccgggcggc
    16201 aaggaaattc agctcgtcca cgtcggcggc cagcaccatg tcatagggaa tggcaaggtg
    16261 tctgtcctcc gggcaggaat acccgttgat aaaaaaatcc tgcggattgt cggcagtaat
    16321 gccaacatcc cgcagggcgg cttgcagcgt ttcggttgtg gtgggcaaat ccagccagac
    16381 gccttgtttg ccctgttcca aaaactgccg gttatggact tggatagaaa atacttcgtt
    16441 cacgcggtca ctcctttctc tgttctgttt caaaaaatag ggggttgggg acttccccaa
    16501 caagcaaaaa tccccggccc ggcagggacg cggccggggg attttaccgg gagtgtagcc
    16561 acactcccta tgcttgctgt gttactgtcc tttagctcct gtgttcaatg cggtagttga
    16621 cgtacttgcc cccggtatca gccaaaagca ccgtcccgtc gtaggttttg ccggtcttta
    16681 cggaatagag gccctttacc ttgactttgc cggttttgag cagcgcagcg gcgatcttcg
    16741 gggtgaacgc tttcttgcgt tcctcaaaaa atcggtcatt cttccacatg acgaactggc
    16801 aggcccggtt tccgcagtag tagtttttct tgccctcata gacagcctca ccacagcggg
    16861 gacacgtccc gataggaaca cgctcggcct gaaacaattt ctgcttttcc tcactgatac
    16921 aggaataggt ctgcaccagc tcccgcgcct gcgtctcgat gccgtccata aattcgtcag
    16981 gatcggcctg ccccttggcg atctcggtca gccggttttc ccattcggca gtaagctggg
    17041 gagaagtaag cgcctccggc agcaccacag ccagattgat gccgtccttg gtgggaatga
    17101 gctgcttgcc ttttctctgg acaaagccca tctgcaccag cttttccaga atggcggcgc
    17161 gggtggccgg tgtgcctaat cccttgcgct cggcatcctc cggcatatcc tcggccccgg
    17221 cccgttccat agcggacaaa agcgtatctt cggtgtaggg cttgggcggc gtggtgaaat
    17281 gctcggtgac agaggcggcc acgtctgcaa aggtctggcc ctcgcaaagc tccggcagct
    17341 cccgcaccgc ctcggcgtcc tcgtcggcgt cggtctttaa ggaagaacgg aaccggcgtt
    17401 caatctcttt ccagccgggg gaaaggacgg tcttgccctt ggcggtaaac tcctgcccgg
    17461 cacaggtaaa tgtggcggtg acggcctcat agatatgcgg cccggccacg gcacataaga
    17521 gcttgcagca gatcagggac agcagctttt tttcactctc ggccagagtg ccaaagccct
    17581 gcttcacaaa ttcagcggtg gggataatgg cgtggtggtc ggacaccttt ttgctgttca
    17641 ggatgcggtc aaactgcggg gaaaggttca ggcccttggc aaacggcacc agctcccaca
    17701 ggccggacac aaggctttcc acggcgggca gcatatcgtc ggtaagatat tgactatccg
    17761 ttctcggata ggtcagcagc ttctttagat acagtgcctg ggcatagtca agggtctgct
    17821 tggcggtata gccgaaaagc cggttagcct cccgctgcaa ggtggtaagg tcatagagct
    17881 tgggcggctg ttccgtcttt ttctcccgcg tcacggaaac gcaaacggcc tgcgcttttg
    17941 cacaagccgc cttgacggtg tttgcctcgg cagcgtcgga aaagcggctg cttaccgcgt
    18001 ccgcgccgcc cgccgtgata tggacgatgt gatatttctc tttcttgaaa tcggtaatct
    18061 tggcgtccct gtccaccagc atttttaagg tcggcgtctg gacgcggccc acagtcaggt
    18121 tcttgtggta caggacggaa aaaagccggg tggcgttaat gccgatcagc cagtcggcct
    18181 tggcccggca cagggccgat tgatacagcg gatcatagtc gccgcccggt ttcaggtggg
    18241 caaagccgtc cttaatagca ctgtcctcca tagaggaaat ccaaaggcgc tcaaagggct
    18301 tttggcatcc cgccatctgg tagacgaaac ggaaaatcaa ttccccctcg cgcccggcgt
    18361 cggtggcgca cacaaggccg gtcacgtcgg ggcgctgcat cagcgaacag agaacgtcaa
    18421 actgctgttt cttgtcgtcg gggacgatgt actgccatgt ttccgggaga atgggcaaat
    18481 cctcatagcg ccattttttg aaacgctcgt catagcgtcc ggcgtcggca aaggacacca
    18541 gatgtccgat gcaccagctc accagatagc cgttcccctc caaatagccg tcgtgtttct
    18601 ccgtggcccc gatcacgccc gcaatgctgc gggccacgct gggtttttcc gcgattacaa
    18661 gtttatatgt cattcctcgt cctcctgttc actcacaaaa tcagcttccg ggtcaaactc
    18721 ggtttcgggt tcctcgtcct ccaaatcctc gtcctcccca aaatcatact cgcccaaatc
    18781 gtcgctgccg gtggtttcag ccttgggctt tttgaacttg aaatagtaaa gggccgcacc
    18841 gccgcccgcc agcgccacca gcaggaacac gatcaggccg cccgcgccgc ccttgggttc
    18901 ttcctccggg gcttcgggtt cctccggctg gggttcctgc ggctccgggc cgctgcactc
    18961 ggtcaggttg gttttacata ccgggcaatt cgtgttcaca gccccggcct tgcatttcgt
    19021 ggtacaggag cagacagcag cgggggtgac ggtttccccg tcctccatca gcgtcagcag
    19081 atcggcttca tccacctgat ttaagaaatg gacagtttct tttccctcgt cgtcccggtc
    19141 aataatcaga taaaacacat tgccgttttt ggtttccacg gtgataaact gcttgccatt
    19201 tttcgcggcg ctgccgatgt cgtcaatcag tgacaggttc ccgtccgggg acagggcggc
    19261 gctggccccg ccgccattga acagttccag cagagaagca atttcctccg ggtcaatgga
    19321 gagggaaaaa ccgccctcgc cggtagccgc tacttccttt tcgctgacgg tcatatagtc
    19381 ggaatggaca tagccgatgc gctccggcaa gagaatttta atccagtcgc cgtctgtgcc
    19441 gactacctca accgtcgtcc cattgggaag ctgggtgaac gcggtcttgt caagccccgc
    19501 gccggtgcgg acattcagcc tgccgccgtt ggtggtgacg gtgccggtct gttcaggttc
    19561 ttttttgtcg gtgccggtgg tgatctggat gccgtcgtcc gtggcggcaa tgctcacctt
    19621 ggaaccgaac agcgcggaaa acaggtcggt gatgtccttg ccggtcagag ggccggtatc
    19681 tttttcggcc tcggtttctg tcccgtccgt ggcggtgccg gtttcgtctg tttcaggctc
    19741 caaaccgccc gtagcgggtt cggttgtagc ctccggcagc ggggccggtg ccgggtcatt
    19801 gccgcctgca agggcggtaa cgggaaaggc cgtgacgcag agcatcacgg ccagaagtgc
    19861 ggggagaata cgggaaatcc gtctggtctt attcttcatg ggcattgtcc tcctgttcat
    19921 ggtaaggggt tgcggcaggg gcggcctgca ttgcgccgct gcgtaagaac gcggcaaatt
    19981 cctccggctt catgttcatg ccgcgcacaa gctggatgat ctgcaaattc tcctgttcgg
    20041 ttttctgcgt ttccagctcc cgaagctgtt tctgaaactc cgcgatctta gacttggttt
    20101 tctggatgtc cttttcgatg cggtcaagtt tggttgccat aattgaaagc tcctttctgg
    20161 cgttaaggta aacgcccgta actgtaaaaa tgggattgcc agtagtttga agtaaggttc
    20221 gtgtaggaaa tcgggttccc gcaatgcagc atcacattgt tgcccacata caggccgacg
    20281 tgggacacgc cgggggtgtc gtaggttccc ttgaaaaata caaggtcgcc gggcttggcc
    20341 tgcgaactgg aaacaggggt gcagatgtta tataggccct gcgcccccag ccgtcccacg
    20401 ttccatccac tatgattgat tacccacgaa atgaagccgc tgcaatcaaa gctggtgctg
    20461 ggagagctgc cgccccacac atagggataa ccgatatatt tttccgcctc tttgagcatg
    20521 gcggcaaagg tggcatcctc caaagcctcc gggggtacgt cgtagtaggt cggcggcgtc
    20581 agggtggagg cgttggggta ctggctgccg gaaaacagat caggccggtt tcctagcgtg
    20641 gacatataca gggaatagaa acttagcgtt tcctcgtcca tgatatagac tggcacatgg
    20701 gacaggtcga agttttccag cgtgacgtag cagatgtaat agtcatacgg cacttggtag
    20761 gtgtctgtat gggtgttgcc ctccgcgtcc gtccatgtat cggtttcggt gcggtaacgg
    20821 gtttctacca cgacgttttc tgtcaggata tattgcttgt caaagagcat ggacaaatca
    20881 ccctgtacct ccccgatggt aaatatcccg tcatggagcg ccgacagaat ggaaatcagc
    20941 acataggggt catggctgat ctcgtcaagg tcaaaatggt attcgtcata gtcatgggtg
    21001 gcctcgtagt tgtccagata gtcttgcagc tcggcttcca gctccgcgta ggcgtcctcg
    21061 gcggccaaca tatcgctatc ctcggaaaag taagaggacg ccgtgatgcc gcttccggcg
    21121 ctgccgaaca tggcggtgca ggactgtaag ccgccgatga taagcagcat caaaagcccc
    21181 aacacaagga cggccagcgc ccctttccag tggcgggcca caaaggaggc cgcccgttca
    21241 ctttctttgg ctgcggcctt ggccgctttc gtggaggccc cggctgtctt ggccgcgccc
    21301 tgtgccgccg cgcctgcggt ctggcccgct gtccgggcgg ccttggcgta ctggcgcttg
    21361 atctgctgtt tctgccagaa ccgggaaatg gggttgctgg ccgcctgtgc gatctccggg
    21421 ttatcccgca aagatttctg gtagaaatac tccgcgttgg cagacaatag cctgcgctcg
    21481 gccttttccg cgtcccagta gggtttcagc ctgtggtgcc ggatggtgct gcggatggct
    21541 tttccggcct gccgctcggc aagttcctcc cccttatggc cgccctccac acccacgttt
    21601 tcatgctcga cttcatggat tttcccgtgg ccgaatagaa aaacctcgga aacaggacgg
    21661 cctgccggat tgggtttcag ggagggaggc cccttgtcct gtttgtcaaa gtgcaggacg
    21721 gtctttgctt tgccgcttgc ctcgtcaaag acggtttcct tgaccgggac gcgcttcttg
    21781 gggatggcgg cgcgggcctt atccagctta tcggcccgtt tgtccgattt tcggatatag
    21841 ggctgtaaat ccgggtcggc ccgttcttcc tccgacagtt ggagccgggc ggatggccgg
    21901 tgcagggcag cgtccccctc ggcggccaca gccccggcct gcttcgcggc tttcttggcc
    21961 ttatggccgt catggatggc gtcggcccgt tccagcagct tatcggcggt gccgcccggc
    22021 cccggcgcgg acaaatcctg ttcggcctcc cggctggaaa tgttctccac ctcgccggtg
    22081 gcctgatttt ccagcgtcag cccgtcgcgg gtcatggcct gcgtcacttt cgccttggct
    22141 ttcagctctt tcatggtggt tcacctcctt tgtggcgtcg ccgggtcata ctcccgccac
    22201 ctcgtttaag cgggtcgtca taatcttgta aagctgggtg ttgtgcggga tggggttggt
    22261 aaagggcagc accacgtttt caaagatcaa aaggccctcg ccgggttcgg aattgtcgat
    22321 gtacttctgc tgttcggtgg acaaattcaa gcgttccgca aggatttttc tatccccgga
    22381 cgcctgattg agcagcgtaa tgaagtcgct gttttcaagg atattttcaa tttccgggga
    22441 ggaaagcagg tctttgacgt tctgggtggc acctgttgga atgccgcccc atttcctaaa
    22501 ccgcttccaa atctccgcag aataggccgc cgtctgttcc tctttcaaaa gcaaatggaa
    22561 ctcgtcggca aaataccatg tggcctttcc ttggcttcgg ttggccgtga cgcgccccca
    22621 tatctggtct tggacaatga gcatccccag ctttttgagc tgcttgccca gctccttaat
    22681 gtcaaaggac acaagccggt tgccgatgtc cacgttggta cggtggttaa atacgttcag
    22741 ggagccggac acatacaggt caagggcctg cgccacccgg tcagcctccg ggacatgctg
    22801 atctaacaac gctttgtgga ggtcggacag gatcggcata ttctccgggc gggggtctgc
    22861 cagatagggc cggtagatca cgcgcacagc ccggtctatc acggtctttt caatggcttc
    22921 cagcccgttc ttgccgccca tgacaagctc acagaacgac agcacaaagt cggatttcag
    22981 ggccagcggg ttatcgtcct ccgaataatt caggttgatg tccagcgggt tcacaaagtc
    23041 cttgctggta ggcgacagcc ggattacctg tccgtggagc cgtttgacaa gggggtaata
    23101 ttcggcctcc gggtctgaaa taaaaatatc gtcaggcgtc gttaagaaca cactgacgat
    23161 ctcggattta cagctcatgg atttgccgct gccgggggtg ccgagcttca aggcattggg
    23221 gcaacgggcc tgcttcctgt ccagcataat catgttgcgg gacttggcgt tgatgccgta
    23281 atacatggcc gcgccgccct gaaaaagctc ctgcgtcaca aaggggacga acacggccac
    23341 gctggaagtc gtaaggctgc gctgtatctt gatctgattg acgcccagcg gcaggctgct
    23401 tgcaaggccc tgttcctgct ggtagtccag ccggacaagg ggacagttat gcttttgggc
    23461 cacgcctgcg gcttggaaca cgtcgttatt cagcttttgc ttggtgtcgg ccacgttgag
    23521 gacaaggaac gtcaactgga acagccgttc attgcgggtc tgcaactttg tcagcagctt
    23581 cttggcgtcc tcgccgtagg tggcaaggtc gctgggaaga atgtccatat cgtaaccgct
    23641 gcggacggcc tttttctgct ccgcaatctt catagcgtca aggtcggtaa ttttgcgctt
    23701 gatggtcttg atcgccttgg tctgttcaat ggcctgcaca tggagattga ctaaaacacc
    23761 gctttccgta ttgaggaagt cggccagcat atcgtccgac agctccgggg acaggatttg
    23821 caggaaactt accgcgccgt atttgccgcc catgccgaat gtctttgcat tgccgaagca
    23881 cagggaggac ggggcaataa aatccttggt ggagaggccg gacggggcca gccacttcca
    23941 gtcaaaattg aatatctcgc cgtccgggtg gtacacgcca tgcagcaggt tcagccgttc
    24001 ccagccgtcc atcacatggg ctacggcccc catgctcttg aaatagccta acaggtcaaa
    24061 gccgatgcgc ttcaatctgg ctcgcgccgc tttcacgctt tcggcctcga tggtgaatgt
    24121 caggtatttg gtctttacca tgccgttgtt gccgtttgca agctgctttt gcagaatgcc
    24181 ggtgtactcg gcccggatgt cgtcgaagtc gtccccctgc ggcgcgatct caaagctctt
    24241 ggcgtactgc accgggtcaa ctttccgatt gaggaaagaa aactggatat gaatggaggc
    24301 gtccacatag ttatacaggt cgcacagttt ttcaaagata gccgtctgcg tgtccggctg
    24361 ggcaagctga tagctgatgt ccgcaaactc aatgcacttg gaaaagacgc cgggcgatac
    24421 ccggcacacg ccgtccgggt acatgacctc gtaggggatg ctctgctgcg ccgtgtgggg
    24481 cctgccgtcg cccttatatt tacggattac cgcgtcaatt tcttttcgct gggcgcgggt
    24541 cagctttgtt ttttcggttt ctcttgggat ttcctttttg gacaatggcc tttacctcct
    24601 gttccatctg gtattgccgc acaagggcgg tgtaggtgtt gtcggtctga tagggacgct
    24661 cctttggctg gatgaagcag cactggatga tctgcataat caccacttca aggggctggc
    24721 cgtgacgctc gtacatggca agcaaaaagc ccggcagcat ggcaaatacc atgagcaggg
    24781 ccgccgcgct gttggtaaga tagtcacggg tgagaaaata taaggggacg ccgatcacca
    24841 gcgccccgga aaaacagatc agttgccgtt tggtaagccc aaacacaacc ttgcttttca
    24901 catgggttaa gtccttgggg acggttacat aagccatttg gcacctcctt tcacgtcagg
    24961 ataaggtcac attccacctc gttcccccac acgtcccagc cgggggtttt ctgtctggca
    25021 aacagctcca cgcggggcaa atcgcccgcc agctctacga ttttttcacg ggtcacatcc
    25081 ggctttttgc tgtgttgttc aatcgggctg ataataaact gatggacaga ggcagaacga
    25141 cgcttcggct tgccgtgtgt tgccagcagg cagatttccg cgtttcccct tgtccagcgg
    25201 cccagcccgt aaaaccatgt aaggctttta cggttctgtt tcagccatac aaaagccacg
    25261 gtcttgaatg aaaagcccca cgctttgatt aaccgcagag cttccggcag catggggaat
    25321 gtggcccaca aaaacagcag gcagtctttg gacgccagcc gttccaccgg cagggcgcac
    25381 agctcgtcaa tgctcatggt ggaatagtga ttttccgctg cgccctgtac gtttttgcag
    25441 tcataccgcc acggcgggtc agcgtaaatg atgttgtacg ttgaaataag caagttcctc
    25501 ctgtgtatgc ttggattttg ggatcaatgc gccccgaata cggcctttga tatactgccg
    25561 gtcttgaaca ggcagaagca cagcagcacc gtatagccca tacagcccca gattgcgccg
    25621 gatatgtcgg aagcggaacc gatattctgt atcagcaccg cgtagatacc caccacaacc
    25681 atgattaaaa aggcttggaa gcccagcgcc agcagggatt tcagataccc ttggcccatg
    25741 ccgcgccatt ccgcgttgcc cattgtggca agggggatag gccccagcga cgtgacaaca
    25801 tagatttcca ccatgcggcc ataggtcaca aggaaaatgc agatggaaag gatattcatg
    25861 gtgaggccca caacaagggt ttggaaccac agccccaaaa gagcgcccac gctcatagcc
    25921 tccaattggc tttcaaggtc tggtatcagg gtgttaaaat caatggatgt ctgcccgata
    25981 atcacaccgg cgctttgatt gacgacgctt tgggtggcgt cgaatacgcc catgacgatg
    26041 ttccatgtgt tcgttacgat caggattgca aacgcggatt tgaatatcca gcggaagaac
    26101 atggagctgt caaagtcgtg catattgttc ttttccgtta tcatctggat aagctcatag
    26161 cacatgacaa gggcgagtat ggccccggct atggggacga tcacggtttc cgacaggctg
    26221 tgcagcatat tgaaaatacc gctgttccat gcctgcgggg ttcgtcccac gtcggcggcg
    26281 atttctccga ctttggcatt taccgtatcg aacagcccgg acaggttgcc cgtgataccg
    26341 tccatgagga tgccttttat ccattcttca atgatttcac taatcacggg tagcacctcc
    26401 tttcccgcgc cctcccggtc tggccgggag gacgcggagg cgattagtta agggtttgga
    26461 cagggacaaa ggccaggatc agcccagcag cccggacagc aggggaacca gagtgacacc
    26521 gatcagggcc acgccgccgc cagcgacaag ctgcttgaca ccctgcgatt ttgcccccgg
    26581 attgtcctgc ccgtatccct ccaacagatt gatgccgccc catacgcaca gagcgccgcc
    26641 cagtccgatc acgatagtct gcaaagtagt aactgcggaa ttaaaaaatg ccataaattt
    26701 acctctttct gccggtttcc ggctcataat gattttttga ttttgttgtt ggaatgaaaa
    26761 aagccgctgt ttattcagcg gcggcgtcct cgtccgacag gtcgatttca tacaggtcga
    26821 atacttcatc ctgcgttacc acttgctgcc gtttccggcg cgtgatagtc aaaaatctgt
    26881 ccacgtcaaa ggtattcttc ttgtcggcgt cggcaaggta tttgtaccgg ggatgcttgg
    26941 taatatcgaa tttctccgat aaaaacgggc gcacaccccg tagctgcaag atacacttgc
    27001 cgccgtccat cacggcaatt tcgtcttggg acatcaactc ctttcctaat ttttgatagt
    27061 tcaatccgtg ggacgtttgg cttccccggt tctcgctggt gttgaaactg tcaatcgttt
    27121 ctttccccag cgcatcggaa atctccttgg catttttgcc gcgcccacta aggaaaagca
    27181 cactatcgca gttatccgaa atgatctcgg cggcgtcctt gtaaatcgct tttagctgcg
    27241 actggctctg caaaataata gaggccgata tttcacggct tcgaatggtg gcaatgagtt
    27301 tatcgaaatt gggtatttgg ccaatgttgg caaattcatc caaaatcagg cgtacatgga
    27361 caggcagccg cccgccgtac acatcatcag cccggtcaca aagcaggttg attagctggc
    27421 tttggagcat agccagaatg aaattaaagg tcgtgtccgt atcgctcata atgagataca
    27481 gcaccgtctt ttcgtccccg atggtgtcca gctccatttc gtcgtcctcc atcagctcgc
    27541 gtacctcccg gatgtcaaag ggcgcaagtc tggcaccgca tgaaatcagg atggaagaac
    27601 gggtcttgcc tgcggacagc aggaatttct tgtactggcg caccgcaaaa tggtcggggt
    27661 ctttctgctc caaacgctca aacatgagat cgacggggct ttgaaactcc gggtcgtcct
    27721 cgcgggcttc actggcgttt atcatttcca gcagcgttga gaagttcatt tcctccgctg
    27781 gggcctcgta ccagatatag ccgatcaggg ccgaataaaa caaccgttcc gacttcaccc
    27841 aaaaatcctc cgctgacttt tccccctcgc ccttggtgtt ggaaatcagg gtatttacca
    27901 gctttagcac gtccttttcc gaatggatgt agacaaaggg gttgtacttc atggacttgg
    27961 aaaagttaat ggtattcagg attttaatgc ggtagcccgc ctgctggaac atcctgccgg
    28021 tttccagaac caaagtgcct ttcggatctg tcaccacaaa cgaagtcgga tagtccgtgg
    28081 tcggaaagca ttgcatcagg ttgggtttca cgaaaaatct cgttttccct gaaccgctgc
    28141 caccgatcac cagcacgttt ttattccgcg ctgtcttggg gtctttgggc ctgccggtca
    28201 tggtaagccg ttccgtttgg gtgagcagga tattattttc aaacttgggg tcgatgtaag
    28261 gcgctatgtc tttggccgtc ccccagcggg cgctgccata ctccatgcct ttgcggtatt
    28321 tcttcacgtt cttgctcttg cagtacacca ccagccgcac gatcacggca ccggcgatac
    28381 cgatacacag gtcaaacagg tgcaggcttg gcagcgggtt ggcaaaagcc gccgcaaaac
    28441 catccgcaaa atgcaggagc ttccccgaca ggtcagcacc agcggccagc cggtacgcct
    28501 ccccgcattt cgcaaacagg tacacaaaaa tcagatacgg gaggttcagg atcagcaatt
    28561 tctttacgtc aggcttcaca gttccagccc cctttccttg attttctcct tggcccgctg
    28621 cttggtctgg gccgccgcct tttgcttcac agcggacaaa accttgcgga tagagggccg
    28681 ctgctccttg gaaagcgtct tggcggtaaa ctcccgaaac gccagatcaa ggttatctgc
    28741 atccttggcc ttgaaaatca caacgtagtg gggcggctgg gtggtcttat cttttcgtaa
    28801 ggtaaaatcc acgccgtatt tcttggcgca tggcttgaac gctccaatgt tggcctcggt
    28861 gatctcaatg ttggagagat cagccccatg ctttttaagc tgctttaggc tctgctggcc
    28921 gtggtgcagc gtgtttttgc ccttggcccg gtgttgcagg tactttttca gcgccgcttg
    28981 cagcacttga gccgtgagct tgcccgtttt catggacagg gcaatggtct tttgagtaac
    29041 ttcttcctgc aatcgcatcc ctccttgtca gaaagtcaaa agcggcgtct aaggcggaac
    29101 caccagacgc cgcaggtaga tataggtcaa atccgcaccc gcaggccgga aaggtcgtcg
    29161 gcccggatgc cgacaagata ccagttttcg gccatttcaa tgcgggtggg cctccatttg
    29221 ccgccgatca tcacgtcgaa acactccccg caatgcaggc cgccgtaata gtcggccaga
    29281 tcaaagcgga tgtcgtaacg gtctgttcgc tcgtcaaaaa tcaatgcgcc ctgcttcata
    29341 gggtcgtcct ccttttctcg ttacaggctt tcgccctcgc cgcaggaata gtccggcagg
    29401 ccggatttag gctttcccca atcgggctgt gccatgtcgt gcgcgacaag ggccgtgtag
    29461 tagttggaaa tggtgctggg ggcgttgaac agcatagccc gcaggtattg tttgatattg
    29521 cggactttcg ttgtgttctc ccgcagacag tcaaggacaa agcggatatg ttcgccggtc
    29581 agcttcatgt acttggattt caccagctcg gcagggtagt cgtccccggc gacacggatt
    29641 gtctttcggg cggtgcagac ggtttccagc atcaggtcta cgatttcgtc aagctgttca
    29701 cggtcaatgc tcctgtcctg aatgagatag gcatactcga tgttctccaa aatgatttcc
    29761 cgataaatcc gtacagcctc atcctttcct gtccttttcc gttccggcgg cgtagccgct
    29821 gcctcgccct ccataggggc aggggtcggg gatgggatag gaatggaatg ggtactttgt
    29881 ccatctgtaa ttattttttc tttttttgat aagtcagttc tttgtacttc tttatttaat
    29941 tgcgttggat tttccgacgc cggattatcc gatgtcggat tttccaacac cgggttatcc
    30001 gatgttggat tttccaatac cgggttttcc aataccggct gttcgtagat ggtatagacc
    30061 atatcggcca ttttgccttt tgcgtcacgg ccctgctcac gccggatata tccagctttt
    30121 tcaagctccc ataccgctgt cctgatcgcg tcgatgcttt cccggttgat ataggacagt
    30181 ccttttaggg tatagtccca atcctccggc aaggacaaca tctgcgacag caggcccttg
    30241 gcctttaagg tcaggttctt attccgcagg tggtgatttg acatcaccgt atagcctttg
    30301 ttcttttcca cacggaaaac tgccatagcg ttgttcgctc ctttcactgt gaatttgacc
    30361 gctgcatagt cggtcagctt gcccggctga tagggacact ctgcaaactg gcagaattga
    30421 tatttccaat ccgggcggta aaatcggcag gtgccgcaat cctccggcgc gtccgccccg
    30481 ccgccgtcgt agtgatcgaa accgggcttt tcctgcatca gccgttcaaa cgcccggtca
    30541 gggccggaag taaatagcat ggcgtcgcct cctttctcta tttgggcata aaaaagcggc
    30601 gtcctgttct cccatgacgg gaaagaggaa cgccgcgttt acggtgtcgt attcaattta
    30661 tgatgtgccg tatttggggt ggcgggtatt agcagtatgg ttcagcatat atgcggctca
    30721 atccgaaagt agtaaattgg tagtaaattg agttctcata tcctgcgaaa tgcccgtatt
    30781 tccggggctt tcagcgataa cctaaatttt tggaataaaa ctttcattat aggctactgc
    30841 aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac
    30901 gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc
    30961 ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata
    31021 ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt
    31081 tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac
    31141 ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg
    31201 ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac
    31261 ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt
    31321 ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt
    31381 ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa
    31441 tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt
    31501 cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac
    31561 ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc
    31621 tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac
    31681 cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg
    31741 tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga
    31801 acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat
    31861 atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc
    31921 gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt
    31981 atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag
    32041 ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa
    32101 tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg
    32161 aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta
    32221 aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac
    32281 cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct
    32341 gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat
    32401 tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga
    32461 tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc
    32521 cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc
    32581 aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta tctctgctta
    32641 tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg
    32701 aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt
    32761 cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag
    32821 taccagccga aatgttccct gtccgtttgg ctacactgtt cgtagcgacc ataccggttc
    32881 ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga
    32941 tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag
    33001 gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat
    33061 ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat
    33121 ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg
    33181 tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag
    33241 cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt
    33301 cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa
    33361 atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct
    33421 cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc
    33481 aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc cactcataac
    33541 taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc
    33601 caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta
    33661 cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta
    33721 gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa
    33781 gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca
    33841 aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa
    33901 aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat
    33961 atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag
    34021 gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg
    34081 gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg
    34141 cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt
    34201 ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc
    34261 ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc
    34321 aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagtgt
    34381 tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt
    34441 tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct
    34501 tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat
    34561 tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt
    34621 tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc
    34681 aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg
    34741 ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg
    34801 gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag
    34861 gcggtagcgt gatgggactt tcattcatcc taccagctgt tttaggaact gtactggatg
    34921 ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt
    34981 gcacatgggc tccactgaca tgggtaatca taggcgaaat attcccacta gctgtccgcg
    35041 ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc
    35101 tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca
    35161 tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt
    35221 tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc
    35281 attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag
    35341 tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac
    35401 ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc
    35461 cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga
    35521 ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta
    35581 caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc
    35641 gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc
    35701 ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat
    35761 agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga
    35821 tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc
    35881 gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac
    35941 acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga
    36001 acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca
    36061 catcatctaa acaaatataa gaggcattta actcttcata agcagcattg atcatcacca
    36121 tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac
    36181 ttttcgtagg ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact
    36241 ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc
    36301 gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg
    36361 tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat
    36421 ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga
    36481 ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg
    36541 gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa
    36601 tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac
    36661 gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga
    36721 acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg
    36781 tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag
    36841 atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat
    36901 tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt
    36961 aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt
    37021 gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca
    37081 tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc
    37141 ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt
    37201 tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg
    37261 tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc
    37321 attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt
    37381 atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact
    37441 tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg
    37501 cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag
    37561 cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct
    37621 gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca
    37681 ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat
    37741 gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat
    37801 ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga
    37861 aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca
    37921 gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac
    37981 aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata
    38041 agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta
    38101 ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc
    38161 gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc
    38221 aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat
    38281 tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat
    38341 cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg
    38401 tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat
    38461 caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac
    38521 gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa
    38581 ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat
    38641 aagcactctt agcccccact tgccatggac cgtctagctc attccctaaa caccataatt
    38701 taacgccaaa aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt
    38761 ttggaaaatt acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat
    38821 tcaccgtgaa tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc
    38881 caaaagaatt tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt
    38941 tcctacctac agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa
    39001 tgacacctaa ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat
    39061 cagctgtttc atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa
    39121 caaatgagcc aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta
    39181 ctttcacttc ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag
    39241 atacaagttt ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga
    39301 gcagtacaaa tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata
    39361 ctttccatgc actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca
    39421 catatatcca ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg
    39481 aaagtacgat tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta
    39541 acagactaag gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa
    39601 tgatatttcc tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag
    39661 caggcacgat agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt
    39721 aatcagaaac cacaatcaac tgtccataaa atttagtctc aactattttc atcttcctca
    39781 ctctttcatt ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac
    39841 cactagtaat ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat
    39901 gttctgccct gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt
    39961 caatatcaat ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga
    40021 taaaagagta ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa
    40081 tccatcaatg cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat
    40141 catgaagata ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag
    40201 catgccaagc aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat
    40261 cattgtcagc aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa
    40321 tgcatacccc gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat
    40381 cactgtattt ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc
    40441 tagggtatag ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt
    40501 aaaagaagaa ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat
    40561 actaatcgtt cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt
    40621 aatcgagctt tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg
    40681 acatgtacac attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca
    40741 gtgctgatgc atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa
    40801 cagctgtttc tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca
    40861 cttgcatcga accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag
    40921 gtagcgtaat gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat
    40981 acaaagaggg agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat
    41041 tcttccaaac agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt
    41101 gagggatcgg caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa
    41161 gcaacataga aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg
    41221 tcgaacgaca aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac
    41281 caacgataat ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat
    41341 taactgctcg ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg
    41401 atgtaaagaa aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga
    41461 cagtaaataa taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag
    41521 gacgattcca atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa
    41581 aatatacaat cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt
    41641 tgcaataaca cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac
    41701 agtctatcct gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg
    41761 catcgttcat gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga
    41821 tgatatcgtt attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt
    41881 ccatttgttt cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta
    41941 ctgaatccaa gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg
    42001 tattccaata tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg
    42061 gaatggcata tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg
    42121 gtaaggtagt cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc
    42181 cattgatttc aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa
    42241 tcgattctgg accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa
    42301 ggttctcagg agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat
    42361 ttgctaacat ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata
    42421 catttttact actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag
    42481 gtggttgttc agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca
    42541 tgccttgtac ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag
    42601 ccgtttcttt aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt
    42661 gtaccgctcc gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag
    42721 gtgctttttt agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga
    42781 tatccatttc aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt
    42841 gcaatatttc accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat
    42901 cagaatcaga ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta
    42961 ccaacaaaga gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat
    43021 aaacgacatc tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct
    43081 tcagctttta catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt
    43141 gcttttgtat caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca
    43201 gacaagtgaa cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt
    43261 tctttgtcat ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc
    43321 gcatagtttg ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct
    43381 tgcataaatg gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt
    43441 ggtgcaataa cgtttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc
    43501 aataaagtga ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct
    43561 agtaaaggag gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac
    43621 caaatattcc attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg
    43681 actgtatcgc acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca
    43741 agataatgag aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct
    43801 atttgatcgt acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc
    43861 cgattcgaac ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta
    43921 gccgtttcct ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga
    43981 ccgtccattt cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc
    44041 gaacgcctta aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta
    44101 cgagctgcat ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca
    44161 tgctgacacc agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa
    44221 gctaaatcca accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat
    44281 cctgacacaa aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag
    44341 tgaatgacgt cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc
    44401 ccttcatata ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact
    44461 ttgcctacac gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa
    44521 tttatacatg aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta
    44581 cttatttttt atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta
    44641 atataacttt tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata
    44701 tatgcaacta caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac
    44761 aaggatcaag atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa
    44821 agaacttgga gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa
    44881 catcatcaaa acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat
    44941 cgatattagt tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc
    45001 tattggtcac tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga
    45061 ttttatcggg aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg
    45121 cattctctgg tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga
    45181 ggatacattg gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa
    45241 tgacaattgg ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac
    45301 gagccctgga gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa
    45361 tcttaatcaa tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa
    45421 tggagaaccc ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca
    45481 tttgcgaatc gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa
    45541 aggattcggt aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc
    45601 ttttctattt tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt
    45661 attgttattt ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct
    45721 tgttccacca tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat
    45781 aatcatcaat ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc
    45841 catcttcaag atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt
    45901 gaagcaattg tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac
    45961 catcaccaaa tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata
    46021 cggctcctgc tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga
    46081 tatttccttt gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg
    46141 catcaacagc tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag
    46201 cagcttttgc atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt
    46261 gatagttttc atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc
    46321 ctgctaaagt agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat
    46381 attttttcaa atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa
    46441 ttgttggttt gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc
    46501 ctatgcttct gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt
    46561 atggttccag cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt
    46621 atactcttcc catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc
    46681 aggtaattcg taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt
    46741 aaagtctcga acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc
    46801 ttcttctcga gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa
    46861 atttcgagtg atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct
    46921 tgataacttg atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa
    46981 tactgcacca agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac
    47041 atcaatttta gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga
    47101 agcatagatt tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga
    47161 tatataggcc atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa
    47221 ttctttcgcc acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac
    47281 taagaacacg cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc
    47341 ataagattgg actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc
    47401 agcacgtaca ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac
    47461 tgctgcatcc agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat
    47521 gacagatttg atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa
    47581 cagtgctgtt tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc
    47641 atttgcaaaa actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt
    47701 ttgtaaatac tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc
    47761 ggaatcattg aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac
    47821 cgcgtatccg tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc
    47881 agctcgacct aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag
    47941 cgtcaaactg tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt
    48001 catttcctac ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg
    48061 aaaaagcaca tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat
    48121 cctttcttat tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg
    48181 agacacgaag aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct
    48241 actacaatcg tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca
    48301 acataatcaa tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat
    48361 ccccaaagaa acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga
    48421 caagatatac cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat
    48481 acaaagtaag aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt
    48541 ctcgccttgt tttttattgt atacattaga tatggagcag gttaccataa agatgattat
    48601 tagtcgttat taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt
    48661 tggacgtttg aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt
    48721 gacatcttct cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt
    48781 gatttttttc ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag
    48841 cagttgtgtt cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa
    48901 gaattccact actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc
    48961 gattagcttc atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat
    49021 gttaccatct ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga
    49081 tttacctttt atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc
    49141 ttgaccatct cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag
    49201 tacacaataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat
    49261 gatgagattg gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc
    49321 aaaaaagaag tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg
    49381 gatgatatcc aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag
    49441 catcagcaaa tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa
    49501 cagacacttg atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg
    49561 tttaaacaac aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa
    49621 tacggaaaaa aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg
    49681 tatgaagaaa tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa
    49741 gaaccagaag atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct
    49801 tacagctggc ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc
    49861 gatgaacggt tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta
    49921 aatgaaatta tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta
    49981 aaatgaccta ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg
    50041 tctgctccat tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa
    50101 acgtataagg tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt
    50161 ctccgcaagc ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca
    50221 aattatcgcg tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg
    50281 ctgcaaccat atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc
    50341 ctgcttgttt tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag
    50401 atggaaaaag ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac
    50461 ttcgccccaa cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt
    50521 ctgtaattga ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt
    50581 cacaaaaaac tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac
    50641 gagacgtaat gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct
    50701 ctttttcttt ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg
    50761 cttcaatgat cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca
    50821 ctccaatgct tttcgttttt ccataactta actgtctggc atttgcatta gggatataat
    50881 cgacttcttt catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc
    50941 cattgattac acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa
    51001 tgtttgccac tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac
    51061 agctggaccg cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg
    51121 cccgccccaa tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac
    51181 tatattccta ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac
    51241 aaacgcataa aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc
    51301 aagccctcct tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt
    51361 gttttgccaa agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat
    51421 tgaatacatt ataatgatac tgtaaatata tctttattat tagtgaatgg atatttactt
    51481 ttaatagata aaatattata actattaaaa ataactattt tcattataat tacacacaaa
    51541 aaagagcgtg agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaaca
    51601 gcggaagcag aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
    51661 tttccgtgaa tttcttctta tttcttggag ttaaacgctt ctgttccagc ctctttttat
    51721 ctgtctttca ctaatgatca gttgtagata agcaagttgc tttttcttac atcataccgc
    51781 ccatcaagac aaacgttttt agcttgtacg tactggacaa aaatcccatt atatcaagaa
    51841 aaaacaacgt tgataatact taaaattatt aggctagaac ggactagtaa gagttttttg
    51901 cccctttttt gcccctcaaa tatcgcttta cagccagaac ataagttcgt ataatcgttt
    51961 tgaggtgatt tttatgatgg aagactttat tagaaaaaat atcagtgatg aatatgcaga
    52021 cttttatgag caaagcagca aaaaagataa attccagatg gatgtttcaa ttttagctat
    52081 attagctttt tccgaaaata aacaacctgt aactgcaaaa aaagaaacag tattctctga
    52141 aggcaaaata aaaactcgat atatattaga ggtagaaact aagtttaaaa atagatcgga
    52201 gtaatggcta tgctttttaa tgagacgcaa ttatggttta aattcgatcc ttcgaataga
    52261 tttgtcaaag atttttataa ggtgtgggat tcagaagttt tctttttagc aatcgaagat
    52321 agcttattaa tcaatctcta ctattctaat aagaactatt tcaaaattcc tgctgcgaaa
    52381 actagaatga agaaggatgt atactttttg tttgatatcg tgactgacgt gccggacgca
    52441 cgtagcgatc atcggcgtta tgactatata aagtatactt tcgttgatcc agaaagatac
    52501 aaagattaaa taaaaaaata acaaacaaac tcgcttgaaa ttcattaggt gagtttgttt
    52561 attatttttt ctgcttctcg attcatcagc gccatatctg ttttcgtaat ggtttcatta
    52621 ggaatatagt cagcacattt tcttttctct aacattctat acccactatt atgtattcct
    52681 aaggtattca attccttaac tgttgaaaac aaattccaaa cggtttggtg gcttcctttt
    52741 ccgctacttc cattataaat aatatcataa ttttctttca aatactcatc agatttttta
    52801 aaagcactgt agtatgctct tgaaacagca gttcttctat aagattcttt agaattatat
    52861 ttatcatctg attcaatttg tttggaaata gataaatatt cattaaagtc aaacatttta
    52921 ttcccccaaa gttgctacta cagatttccc catatattta tagtcagata atacattatc
    52981 gagagtatcc aaaacatcgt aatctgagga aaacaattca gatgttgttt tgataacaaa
    53041 gaaatacaag tttaaatctt catcatattt tatattgatt tcagcttttc tttttaaaac
    53101 cgtatttatc acatcaaaaa ttttgccatc attacttgaa attgtgttaa tctcattttt
    53161 tgtagtaatt acaagttgat tatcattttt agttttcgga gtagaacttg catccacaac
    53221 aactggagat gctagaatta ccgatgcgca agcaaaaatc gcagcaaaat ttattaagct
    53281 accactccga tgtgtatttg atggtactat atcaagcatc cgtatcgtct ccttaatttt
    53341 tttccactgg tttgttgacc ttgatatcaa agatttgcct tccaatctca gttccattaa
    53401 atttaacaac agcataatgc tctcctgctt cttcaaccaa gacatttctg gcgtttatat
    53461 taaagtttat atcgttaact tgaggaggca agcttaccga tacttcaccc gtagaaaaaa
    53521 tttgtttttc attcttgttt aaaacttcaa tttctactat ttttatcttt tctgtatcct
    53581 ctatctcaga caccacagct gttacagaaa atgataaagc agtaggaatt aaaggcgcat
    53641 taatagaaag agctggatta attactttat taataacatt agtgccaaat tgctgacttt
    53701 ctacatcttc tgaaattatt aattgtactc tataagacat tgatttctcc taatttttac
    53761 atttatttaa taaatttatg tttcactttt gctagtcaat ttagtttaac aatagtaaca
    53821 aagctttgtc aacggaaata ttagctaatt ttgtaagcat atttataagt atatttgtaa
    53881 gcatatctga catttaatca gtagtaaaag tgcctctata taaggaacaa actgtttttt
    53941 tactatctaa attcgtatac agttttacta acaaattagc taacaatatt agcaactata
    54001 tcaacaacta tatgtacttc ccctcaaaaa tgaggggcta tttttatcgt tgcggaatat
    54061 ttaaatacca gcgtttatca tgaaaatctt gtgctccgcc tttagtgttc ccttctggat
    54121 cattcgttgc ccgcatcatt acatagactt tcttattagg gaagttgcgc atgttaaaag
    54181 atacatgata gccaacattt cctaaagtat tataagcttg atttacatct ggtctatata
    54241 ttccatcagc ttttactcga gctaattctt tcccagtatt gtaatccata atgaagatat
    54301 actcgtattt atagttagcg atgtgccaac cagccacatg taagtttgcg ttttcgattt
    54361 ctccgaactg atcaatgtgg gcgtaatttg ttccatctgt cagggtagga tttgcagcac
    54421 ctgctcgagt tggatcaatg actggtttat catctgaagt tgtcggattt tcatcggtaa
    54481 atccatgagc caaatcatag gctaattttt ctttacttac gcccatttca gaaagataac
    54541 cgtaaggatc tgtatgatcg ccccaaatat tttgtgttac ccataaatgc gatttgatcc
    54601 ctggttgatt atacggtgtg tctagtgtaa gtggaatacc atatttcatt gctgaatctc
    54661 tagctaattc aacgtatgcc ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt
    54721 gtaactcaat ctgcacggga ctgttagcat tagcatacga accagcaccg tactgtacat
    54781 aaccaggttg tccgacttgg taaacaattc cgccgtctcc cacaatataa gcagtgtaag
    54841 cactagtcca tgaacgtttc atatactgcg cttcattgcg ccctgttgct gtttcgttag
    54901 ctgtctcatg cagtaaaatg tactgattat ttgctacttg agagctacct tcgtttgggc
    54961 ccaaattaaa ttcattgttg atcgtataag caaacgtatt aatggg
//
LOCUS       DAZUJO010000011        51594 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000011 DAZUJO010000000
VERSION     DAZUJO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.011"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..1722)
                     /locus_tag="ACG8OL_000733"
     CDS             complement(22..1722)
                     /locus_tag="ACG8OL_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816740.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2229..3389
                     /locus_tag="ACG8OL_000734"
     CDS             2229..3389
                     /locus_tag="ACG8OL_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4816741.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3405..3848
                     /locus_tag="ACG8OL_000735"
     CDS             3405..3848
                     /locus_tag="ACG8OL_000735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4816742.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4331..4861)
                     /locus_tag="ACG8OL_000736"
     CDS             complement(4331..4861)
                     /locus_tag="ACG8OL_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS4816743.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4966..5517
                     /locus_tag="ACG8OL_000737"
     CDS             4966..5517
                     /locus_tag="ACG8OL_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4816744.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5670..6143)
                     /locus_tag="ACG8OL_000738"
     CDS             complement(5670..6143)
                     /locus_tag="ACG8OL_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4816745.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6281..7753
                     /locus_tag="ACG8OL_000739"
     CDS             6281..7753
                     /locus_tag="ACG8OL_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4816746.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7750..8460
                     /locus_tag="ACG8OL_000740"
     CDS             7750..8460
                     /locus_tag="ACG8OL_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS4816747.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8812..9096
                     /locus_tag="ACG8OL_000741"
     CDS             8812..9096
                     /locus_tag="ACG8OL_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS4816748.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9413..9796)
                     /locus_tag="ACG8OL_000742"
     CDS             complement(9413..9796)
                     /locus_tag="ACG8OL_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS4816749.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(9798..10382)
                     /locus_tag="ACG8OL_000743"
     CDS             complement(9798..10382)
                     /locus_tag="ACG8OL_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS4816750.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10429..11904)
                     /locus_tag="ACG8OL_000744"
     CDS             complement(10429..11904)
                     /locus_tag="ACG8OL_000744"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS4816751.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12085..12396
                     /locus_tag="ACG8OL_000745"
     CDS             12085..12396
                     /locus_tag="ACG8OL_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816752.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12511..14847)
                     /locus_tag="ACG8OL_000746"
     CDS             complement(12511..14847)
                     /locus_tag="ACG8OL_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS4816753.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            14989..15657
                     /locus_tag="ACG8OL_000747"
     CDS             14989..15657
                     /locus_tag="ACG8OL_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4816754.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15660..16703
                     /locus_tag="ACG8OL_000748"
     CDS             15660..16703
                     /locus_tag="ACG8OL_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4816755.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16940..17821)
                     /locus_tag="ACG8OL_000749"
     CDS             complement(16940..17821)
                     /locus_tag="ACG8OL_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4816756.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17980..18708
                     /locus_tag="ACG8OL_000750"
     CDS             17980..18708
                     /locus_tag="ACG8OL_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS4816757.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18864..19181
                     /locus_tag="ACG8OL_000751"
     CDS             18864..19181
                     /locus_tag="ACG8OL_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4816758.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19174..19782
                     /locus_tag="ACG8OL_000752"
     CDS             19174..19782
                     /locus_tag="ACG8OL_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS4816759.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19837..20628
                     /locus_tag="ACG8OL_000753"
     CDS             19837..20628
                     /locus_tag="ACG8OL_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS4816760.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20766..21254
                     /locus_tag="ACG8OL_000754"
     CDS             20766..21254
                     /locus_tag="ACG8OL_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS4816761.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            21305..22177
                     /locus_tag="ACG8OL_000755"
     CDS             21305..22177
                     /locus_tag="ACG8OL_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4816762.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22353..22715
                     /locus_tag="ACG8OL_000756"
     CDS             22353..22715
                     /locus_tag="ACG8OL_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4816763.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22724..23476
                     /locus_tag="ACG8OL_000757"
     CDS             22724..23476
                     /locus_tag="ACG8OL_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4816764.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23490..25292
                     /locus_tag="ACG8OL_000758"
     CDS             23490..25292
                     /locus_tag="ACG8OL_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4816765.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25569..26609
                     /locus_tag="ACG8OL_000759"
     CDS             25569..26609
                     /locus_tag="ACG8OL_000759"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4816766.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26661..28805)
                     /locus_tag="ACG8OL_000760"
     CDS             complement(26661..28805)
                     /locus_tag="ACG8OL_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4816767.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(29162..30535)
                     /locus_tag="ACG8OL_000761"
     CDS             complement(29162..30535)
                     /locus_tag="ACG8OL_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4816768.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACG8OL_000762"
     CDS             complement(30535..30930)
                     /gene="rbsD"
                     /locus_tag="ACG8OL_000762"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS4816769.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACG8OL_000763"
     CDS             complement(30958..31875)
                     /gene="rbsK"
                     /locus_tag="ACG8OL_000763"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS4816770.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(31983..32996)
                     /locus_tag="ACG8OL_000764"
     CDS             complement(31983..32996)
                     /locus_tag="ACG8OL_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS4816771.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(33077..33676)
                     /locus_tag="ACG8OL_000765"
     CDS             complement(33077..33676)
                     /locus_tag="ACG8OL_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4816772.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            33862..34563
                     /locus_tag="ACG8OL_000766"
     CDS             33862..34563
                     /locus_tag="ACG8OL_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4816773.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            34573..37284
                     /locus_tag="ACG8OL_000767"
     CDS             34573..37284
                     /locus_tag="ACG8OL_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4816774.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            37735..39363
                     /gene="treC"
                     /locus_tag="ACG8OL_000768"
     CDS             37735..39363
                     /gene="treC"
                     /locus_tag="ACG8OL_000768"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS4816775.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(39423..40604)
                     /locus_tag="ACG8OL_000769"
     CDS             complement(39423..40604)
                     /locus_tag="ACG8OL_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4816776.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            40856..42379
                     /locus_tag="ACG8OL_000770"
     CDS             40856..42379
                     /locus_tag="ACG8OL_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4816777.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(42448..43176)
                     /locus_tag="ACG8OL_000771"
     CDS             complement(42448..43176)
                     /locus_tag="ACG8OL_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4816778.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(43189..44469)
                     /locus_tag="ACG8OL_000772"
     CDS             complement(43189..44469)
                     /locus_tag="ACG8OL_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4816779.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(44610..45212)
                     /locus_tag="ACG8OL_000773"
     CDS             complement(44610..45212)
                     /locus_tag="ACG8OL_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4816780.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            45457..45663
                     /locus_tag="ACG8OL_000774"
     CDS             45457..45663
                     /locus_tag="ACG8OL_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816781.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            45975..46694
                     /locus_tag="ACG8OL_000775"
     CDS             45975..46694
                     /locus_tag="ACG8OL_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4816782.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(46775..47458)
                     /locus_tag="ACG8OL_000776"
     CDS             complement(46775..47458)
                     /locus_tag="ACG8OL_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS4816783.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            47677..49863
                     /locus_tag="ACG8OL_000777"
     CDS             47677..49863
                     /locus_tag="ACG8OL_000777"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS4816784.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            50209..50619
                     /locus_tag="ACG8OL_000778"
     CDS             50209..50619
                     /locus_tag="ACG8OL_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4816785.1"
                     /translation="MIKVYTSPSCTSCRKARAWLKANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            50633..50989
                     /locus_tag="ACG8OL_000779"
     CDS             50633..50989
                     /locus_tag="ACG8OL_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4816786.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        16852 a         9073 c        10262 g        15407 t
ORIGIN      
        1 ggatgtattt atcccttttg cctaagcacg taaaagcttg tatctatgat aaataaataa
       61 acatagcaag gtaaaccagc caatcaaagt aagagaaaga atgaaataga aatagattgg
      121 cgtttgataa tgaagcgtga gctcgttgat tcctttttgc tgcttcacga ttggggtccc
      181 gatatctgtt acttcataat cagtcaatgt ttttccattt tgctgtagca cagtccgatc
      241 gtagacgata acagggatat gaacagtttt gtccccagtg tttttccact tgattgtcaa
      301 aagaccgcct ggatgtgttt ttataaaagt tgtttcgttt tctagtattt cttcgttata
      361 ccggtcgtat ttattgtctt ttgtctcctt atagattggc agatagtcag gtgtggtttt
      421 ttggaaaacg aacaagctgg tagacatatc tttgctgaag aatgttttcc ttgcttcttc
      481 cggcgtatcg aatagataag tatgtcgact gacaaatgtt tcattttccc acttttccag
      541 atgctggtat aagttctgga tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa
      601 aagacggaac cacttttgtt cagaccactg gttcagtgaa agggcaaggt aaagtagaag
      661 caatagcgta aatggcacga aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac
      721 atttttttcg atcaatgtcg tccatggaat caagctagta gaaagaaaaa agaaaacaag
      781 acttgtgtac aacacgattt tttggaataa ggtgttcttt tgttgaaaga aaataaaaag
      841 caaaaaacct gataatatca atataggaaa ggccttaggg taatggagcc aataactgcc
      901 atccagatct atcgcctttt gactcatttc tctgttaaca aagggaggaa gaatcttatt
      961 cccactgtaa acaatagcta atcctgccca aatatttgct gtcagaacaa aataaagcaa
     1021 gaccgactta atcagttgaa gcagtgcttt tcctttttca gtctggttaa aaaagtaata
     1081 aagataaaaa gggatataca tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt
     1141 cataagtccc actgagacag cgactttgat aacagggaac tttttatgaa ggacgaaatc
     1201 agcaataggg atcatacata aaggataaaa acaagtcccc cagctagtga atccttggtt
     1261 gatagaccag tattgaatag aatacgtact catgaataaa acggctactg gtacacttac
     1321 ccaatttcga acattgacat atctcagtaa aatcagcata gaaaatcctg aaataagata
     1381 aagaatgaaa tttgaaagaa tctgataatg aaaccagcta cctgcaagca acacgatcaa
     1441 tccttgtaaa taagcgaaaa atgggccgta aacggcattc acgatcctgc cactttgctg
     1501 gaatccataa agggaaggaa aataatggaa atccccatat ttaatttgtt gcgcggcatc
     1561 atagaaccga ttgtaatgga atggggcatc agcacccaaa attagtttat gtgtgaataa
     1621 atgaggcatg agaaatgcca aagcaatgaa aaaaattgct aaaaatgggc catatttttt
     1681 caattgtcga actcctttgc aaactttaga taaattcatc atatatccta tgcgagaggt
     1741 aggcaagaaa ttagaaaatt catgaaaata tttatgaatt ttacaagaaa aacataagtg
     1801 aaagttctaa ggagagagga tgcgacataa gttttgttgc atctccttgt tttgaaacag
     1861 aatttttctt aatactcgaa acaaagtagc tttttcatga cctctcggta aacggtgttc
     1921 aatcagctgc ttctcggtaa taagctcact agcctagaaa atatgagaag ctattttctg
     1981 actagcgctc ttattactca aagcataacg cagttttcac aacctcttcc ttaaacggtg
     2041 ttcaaaagct tgcatctgcg gtaataagcc caacccaaca aaaaatatga gtagctattt
     2101 ttttgggctg tacttattac ttgatgcagg acagcttttt cacaacctca tcggctttca
     2161 ttttctattt ttttcctgaa cccatataat aaaagaaaga acaaaaaaac ttgcaaggga
     2221 ggacacatgt gaagcagagt aagcaaaaac agtctcgtcg acttttgttt cgtttgtttt
     2281 tattaatatg tatcgtcgta gggttactga tcgctttata tccattttat gtggattctt
     2341 taaatagttt tattgatcaa aaacgtatcg aacaagtaca aaaacggacc gcagctgaaa
     2401 ttgaagtgca gcgaaagaaa atggaggaga aaaaccaacg attaactgac caaggattca
     2461 atcctggtac tgatcctttc aatgaaaaga acagaaatga aagtacaaca tccagccaat
     2521 tagaggaatg gctgatcgga agtatcaata tacctaagat ccaattaaat atttcacttt
     2581 atgatcggct caatggtagg atccttgaaa atggagcagg cgtcttgcaa ggaacctctt
     2641 tcccgcttgg tggaaattcg acgcattcag tgatctctgc tcacagcggt ttacctaatc
     2701 gacggttatt taccgaatta gatcgtttag aaaacggaga cacgtttatt ttgactgttt
     2761 tgggggagaa attggcttat caagttaaaa atatacaagt ggtactgcca gatgacacgt
     2821 ctgttctaac gatttcagaa ggagaagact tagttacctt gcttacatgt acgccgtata
     2881 tgatcaacac gcatcgtttg ctagtgacag gacatcggat tccttataac gaatcggtaa
     2941 aaaaagaaga aggaaaagga aatcaggaaa gaaccatgcg gcaatggttt attcttgcag
     3001 gaacgataat agctgtagtc atactgttag tgtttatcgg acgactaatc tatcagtata
     3061 gattaagtaa aaaagtgctt gatttttcat ttattattag tgattcgtca gggaaaccgg
     3121 taaatggtag ttcattcatt ttgaaacata agaaaaaaac acttactaga aatggtgtgc
     3181 cgttttcagt acaaagcgac catcatggga aagtcaagct ggatcaattg cctggtggaa
     3241 cttatcgaat cgtttcagct gatgatccta aagttgctgt ttcttttggt attagaaaag
     3301 taaaacaaga gaagatatac ttttttgagg ggcgaaaact tgtaaaagag cttcaaaaaa
     3361 atggttttta ttttaaaatc aatgaataag taaggagaga aaaaatgatc cgcacaacaa
     3421 caaaattgtc tgccaaagaa ctggaatctg ttctatcgat ttggctgcat gctaacttgg
     3481 attctcatga attcattcca tcaacttatt gggagaatca agtagaatct gttaggaaag
     3541 ctttaccaga tgcagtgatt tacttagcag aaatagatca agaaattatt ggttttgctg
     3601 gggtgattga tcagtttatc gcaggaatct ttgtgaaaag ggaataccgg caacaaagaa
     3661 tcggtacgca actaatgaaa aaaataaaag aaaccaataa aacactgact ttgacagtct
     3721 atcaaaaaaa tcaatcagct attcatttct atctttcaca aggttttaca attcaaacac
     3781 agcaaaaaga tgaagcagta aatgaggagg aacttgtgat gaaatggaat caaaacgctg
     3841 atttttgatg aacagaataa cgaaataatg ctcccagcct aattttttgc tgggagcatt
     3901 atttcatttt gaagtcacta atttaactgt gtgaatatgc agttgttaca atctttgata
     3961 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     4021 tatttttcca tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttgat
     4081 aaacggtgtt caaacggctg cttctcggta ataagcccac tagcctagaa aatatgaaaa
     4141 gctattttct aactagcgtt cttattactc aaagcataac gctgatttca caacctcttg
     4201 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaacatgagg
     4261 aactattttt ccgtttcttt ctctaatact caggtcagaa cgctgatttc acaacctctc
     4321 tttcacaacc ttattttact cgtttataat tttcatcgaa gtattttccg ctacggatat
     4381 catctggcat tccatcgtat ggttcttcgc taataacatc ttcattgtct atacctgcat
     4441 cacccatata agtgatcgta gcaaattcag gaacaagtaa ttttggataa gtttcatatt
     4501 tttcacgagc tgcttccatt tctgcgatat gttcgttttg gaaagtaaca gtgatttctg
     4561 gatgttcttc aacccattta gggaaaaaga tcattccgtg aagtttcttt tcatttggca
     4621 taaagtccaa tgccacgtct gtccctgttg gctctgtcca agtgattttg aatacgccgt
     4681 ccgtcaattt aacgatatca gcttcttggt cttttaccca acggcctttg accattccgc
     4741 catggatacg gtaatcaaca gtatggtcat ttttggcata ccattcatat tcccaaccat
     4801 tgtcatacgt atagataaag tgagttccaa taaaatcttc caattcttta aattctttca
     4861 tgtttaagca tctccttata aattaaaaca tgtaataaac tacatgtttt cgattactca
     4921 tttatgatag aatggattta aagatttgtc aaggagggaa aactgatggc aagaaaaaat
     4981 acacttcgat atattttgtt agggttatta agtaagaaga agatgacagg atatgaattg
     5041 aatcaatcgt tcaaaaatga aataggagaa ttttggcaag caaaacatag tcagatttat
     5101 cctgaacttg caaaaatgga agagcaagga atcattcagc atcaagtaga gatcacaggt
     5161 gaaaaattgg aaaagaaagt ttatgaatta acagaagaag ggaaggaaca attggcagag
     5221 tggattcata cacccaccaa tgaactaccg gttaatcgag acgaatttgt tctgaaattg
     5281 tatttcgtca aagacgtgaa tgatccggca ttagtagaaa ttattcagca gcagcgtgac
     5341 cttcatgaag aaaaacgact tcatctatta gagcgaaaaa aaacgatctt tcctactgaa
     5401 aaagaaaaac atgaaaatta cggacactac ctgattttag cgcatgcgat caatcgggaa
     5461 acagaatatg caaattggct aacagatgta ttagaagaaa gtaaccgaaa aaaataaaaa
     5521 aatatccgaa cagtgatgga gaattccttt ctatatgaaa gaaacgcaga tcaccgttcg
     5581 gatattctta tgttcaaaca ggcctttttt caagtattta tgtagcatct acttattaaa
     5641 aagcttggta cttaggagat tagtggcgtt tagagttctt ttaacaactg ttcgtagtca
     5701 gctttttcat cgacaggaag gagcttgtaa gttaattcgt ctgtctcgtt gtcatgaatg
     5761 atgtacatct tagcattctc ttctttgaag ttaccgattt gtaaaatcgt atagcgttcg
     5821 tcgatcaatt cgatatcttt gattgctttt gaaaaaactt tacggacatg gtgcattttc
     5881 ttttggcgga tttctgcctt ttctgcagct tttgacaaag taaatccgtc atctaaatat
     5941 tttttgatca gttctacttt tatgactgta ggtaaacgat attttctagg acaattcgca
     6001 tcagcttcga tcgaacgaat gaatcccttt tgttcccaat accttaattg acgtggagac
     6061 acacctacga tatcacttaa ttcactgatg ccaacaacca attgatcatt ttccaacaaa
     6121 cgtttaaggg aagccccaac catttttgtc actccttttg tcctcttgct tcccttaatt
     6181 atatccgtat taacttatgt gtcaagtagg ttgacaaata gcgaatttag gactaaagta
     6241 ttctagtagt ttcagaaaaa attcagaaag aaggagaaat atgagtaaca accaacccgt
     6301 agatatttac ggaaaaccat ataatcggac acttttagtt gttgtcttat taattggtac
     6361 attttgtacc gttttgaatc agaccttatt gacgacagct tttcctacat tgatgaaagc
     6421 tttcgatatt tcagcgtctg atgtgcaatg gttaacgact ggtttcttgc tagtcaacgg
     6481 gatcatgatc ccaataacag catggctgat caataaattc agttcaagaa atttatatct
     6541 ttctgccatg acgatctttt tgatcggaac gattacttgc tttacagcac ctaattttag
     6601 tacattacta atcggtcgtt tgatccaagc ttgcggtgtg ggagtatcga tgccattatt
     6661 gcaaaatatc atgctatcta ttttcccgcc agaaaaaaga ggatcagcaa tggggatgtc
     6721 agggatcgtt attggacttg ctcctgcttt aggccctaca ttatctggtt atatcattga
     6781 taactaccat tggcgtgact tgtttggtat ggtactgcca atcgttgttc ttgtcttggt
     6841 tttagcattc ttcctaatga aaagtgttat tccattgtca aatcctagca ttgatatttt
     6901 atccgctatt ttatcaacga ttggattcgg tagtttactt tacggatttt ctagtgtagg
     6961 agatgacggc tggggaagct tgaaagtaat tggtttctta gttgttggtg tgattttcat
     7021 cgccttgttc tcatggcgtc aattacattt ggaacatcca ttcttggaac ttcgtgtatt
     7081 caaatcatct acattcacga ttgcagctgt tttagctggt gtaacaaata tggcgatggt
     7141 aggggcagaa atggtcgtac cattgtatat tcaaaatatt cgtggagaat cagcattcca
     7201 ttctggcttg atgttgttgc cgggtgcatt gatcatgggg gctatgatgc ctgtgacggg
     7261 tgcgattttc gataaatacg gcgcgaaacg tttagccatc acagggatgt tcattttgac
     7321 agcggcaact gcaccatttg ctttcttgac aaaaacgact ccagttttat atatcgtgat
     7381 cctttatgcg atccgtatgt ttgggatctc catggtcatg atgccggtaa cgacttctgg
     7441 tatgaatgca ttgccaaata acttgatcag tcatgggaca gcggtaaata atacattccg
     7501 tcaaatcgca agctcgattg gtacagctat tttgatcagt gtgttgacga atgtgacaaa
     7561 agcaaatatc ccaagcaaat ctgtattgga taatacgcca ttagcttata aagatcaggc
     7621 aatcaatgcc acactgtcag gttaccatgc cgcgtttatc gtagcgatca tctttgggat
     7681 tcttggcttt attattgcct ttttattgaa gaaaaaagaa cctgttctaa tgaaagcggg
     7741 ggataatgca tgattattgg agttttgatt gtcagcttgc ttgcttttgc tttaacgaac
     7801 gtgttcgcta aaaaaacatg gcaaaccttt ttatctttga ttttcggact aatcttcgtc
     7861 gcttcactaa gtttgatcgt cgctaatttg tccaatcact ttggaatgga aaaagtaacg
     7921 gaaacaaaaa cagaaaaaat tgtttccagt gctgattccc aaggagcaga tatgcttttg
     7981 tataaagctt tagggaatgg aaaagaaaaa gtgtatcttt accgaacaaa tgaaaaacaa
     8041 gaaaaaccaa aagcaactgg tacggataac gaaacaaata aagtagaaaa gacagacggc
     8101 gatgctgaaa aagtgacgaa aactacttat tgggaataca aaaacgacat gtataaattc
     8161 tggttcaata ttgctgataa caatcacgaa tatgacagcc gagtgaatac attcaaaatc
     8221 ccagagacat gggtagaatt aagtacggac caagcagcca agttagctga attagtgaag
     8281 aaacagcaaa gtacgatgga aagtgaagca aaagcttatg ttcaagacgg tatggtaaaa
     8341 gcaatgactg aaaatccaaa aatgagcaaa gcagagcaag aacaacgtac gaaagcctta
     8401 gctgctgaat tccaacaaca agcattcgca aaattagtga aagaagcaaa aggcgaataa
     8461 tattttgaac accatgtagg aggttgtgaa aaagctgctc tgcatcaagc agtaagaaca
     8521 atcagataaa aatagcactc catatttttc atctgattgg gcttagtgcc gtagatgcaa
     8581 gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactct gagtcacaag
     8641 gagcaatttc aaaaaacagc ttctcatgtt tgttgactat tgtgacttgt gaccgaggag
     8701 ttggcaggag aacaccgttt attcgaaaaa aggaactgcg acaagacttt tgtcacagtt
     8761 ccttcttttt tttgctatgc tatactagat gtaaaaaata ggagaaataa aatgagggaa
     8821 atttattctt ttgaatcaaa aatctatgct tccgaagtcg gcaagggcgg ggcatatatt
     8881 atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt ccaagcaact
     8941 tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa tccagatggt
     9001 tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg aaaagaaatc
     9061 ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca aaagacgtgt
     9121 cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt caaatttatg
     9181 caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg aagcggcttt
     9241 atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag ctcaaaaacg
     9301 gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca taacgctgat
     9361 ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact ggttatttga
     9421 tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct tctgacatcg
     9481 gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg gaagtgatcg
     9541 tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct aagacatcgt
     9601 ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct gtatcacgag
     9661 aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg atcaataagt
     9721 ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt acttcaacta
     9781 tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt tacaatttca
     9841 aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt ttgaggctga
     9901 ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa tttagtcaag
     9961 ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa ctttcgttgc
    10021 tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc aacagagata
    10081 cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa aaatgatccg
    10141 ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag gctttcgaat
    10201 tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg actgatgatt
    10261 ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat ggcataaatg
    10321 gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc caattgttcc
    10381 attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt atttcaaata
    10441 tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt tcatatcgaa
    10501 aggaactgta ccgtcaaaga tagtttttgc acttatccca cggaaacgaa tagagccagg
    10561 attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg ggatcacgat
    10621 gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt tcatgtcgaa
    10681 aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg ctgataaagc
    10741 aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac ggatttgcat
    10801 gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta cttttcctgg
    10861 aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg ccatagatac
    10921 gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc ggtgagtaac
    10981 tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc caggaaattc
    11041 tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat ttggcatgat
    11101 ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg ctataccatc
    11161 aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat tgtagccaag
    11221 tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta aaccaatatt
    11281 gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt gaccaatgtg
    11341 gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa ccatacgatg
    11401 gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac ccattgtgat
    11461 gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc gaatatcgaa
    11521 gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt tgtctacttt
    11581 cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg ttcgataatc
    11641 atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt tcacacgtgt
    11701 gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag tcggacgttt
    11761 tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt caggatcgat
    11821 ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga tgacttttct
    11881 taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta cctatctata
    11941 gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc tacaatggaa
    12001 tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa aagaaagcgc
    12061 atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc taaagcagta
    12121 aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga agtaaaatac
    12181 gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa aaaaggaaga
    12241 ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac tgaagtaacg
    12301 attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc agaagagttg
    12361 tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa aaacagcgcg
    12421 aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca gcgctcttat
    12481 gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga tagcggcgtc
    12541 ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata tagtagagac
    12601 gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta gatccgtcat
    12661 gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca cacctaatat
    12721 cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga tgtctttttt
    12781 acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca cagatgtata
    12841 agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa aagcaataag
    12901 gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc cagctaaatc
    12961 agtataaatg attttatctt catctgattt gcctttattg taatcatcta gataatccaa
    13021 gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag gcaagctgtc
    13081 tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt cagtagttaa
    13141 gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt tttcgttgac
    13201 gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat tcattgtaga
    13261 agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat tctgatacat
    13321 cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat agtaagtatt
    13381 gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt tttcgtcttc
    13441 tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat tgccgtctac
    13501 gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag aataattgtc
    13561 ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca ctcccacacc
    13621 tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat tttgattcga
    13681 gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc ctgtagtacg
    13741 ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat cgacgtatgt
    13801 ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg cagtaatcgt
    13861 tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac gagtctggtt
    13921 agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt gcgtgttatc
    13981 gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga tgatcccaat
    14041 actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat tgttgaagga
    14101 aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt gatcatcttt
    14161 tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa attcaatgat
    14221 tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca ctaatttttc
    14281 tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat ctaacgctcc
    14341 ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag cgatggccac
    14401 tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat ttgtcaagcc
    14461 gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa caccacttaa
    14521 tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt tgtaactttg
    14581 aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct taaagtcttt
    14641 agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc caccaatcac
    14701 attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga attctttttg
    14761 tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga ctttataata
    14821 tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa atattcggta
    14881 tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta actgaagatg
    14941 aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat gaatcgtatt
    15001 ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt ggaacgagcg
    15061 ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact agaagcgaca
    15121 cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta tgacttgcta
    15181 gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc aaaggccgat
    15241 tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac aaaacccatc
    15301 gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa gatcaattac
    15361 agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac agtgttacaa
    15421 gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa attactgtct
    15481 tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg attagatacg
    15541 aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg ttttgaaaag
    15601 acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt tatttaaaga
    15661 tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc ttcatttcga
    15721 tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga cattctatct
    15781 cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt gcggggttca
    15841 gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg catccaatca
    15901 gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt cgaatggatg
    15961 aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt atggtccaag
    16021 aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc catgaattca
    16081 aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca aatctctctg
    16141 acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag ctttcacaat
    16201 taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa ctggaaaaga
    16261 aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag cctaaatggg
    16321 agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca ggcaatgaag
    16381 agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac aatcaaatca
    16441 atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg gaagtgaccg
    16501 attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa ttttatcaag
    16561 gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt aaaaaaattc
    16621 tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga acgacagcaa
    16681 tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc tattgaaggt
    16741 ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg ttcttttggc
    16801 ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca ttgaaacaag
    16861 gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg ggtggataga
    16921 tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa aatgtatctg
    16981 aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct gttgttggag
    17041 aaatagggag aatcacagta ttttctttta atagattctt gactgttttt tccataacaa
    17101 tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt gctcctttat
    17161 aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt tgatacaagt
    17221 tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga tccaatttct
    17281 ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct gtttcagtag
    17341 gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg atcgtgtcac
    17401 tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc aactggactg
    17461 taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta taattgatca
    17521 ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag gtttcaagca
    17581 cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc aataagcttc
    17641 cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg tctaattctg
    17701 tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt cgatcagcag
    17761 ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta tggatatcca
    17821 tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg aattggtgta
    17881 atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag aaatttttct
    17941 gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg agttttgatc
    18001 aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa taaattaagt
    18061 gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg gatgggaatc
    18121 tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt gataggaaca
    18181 gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc gctgatgcaa
    18241 gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga tgaatttatc
    18301 agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat ctatggaagt
    18361 ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt ccttgatttt
    18421 ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat agcaattccc
    18481 caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc attgactaca
    18541 ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc taccggtttt
    18601 agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat gatcgttatc
    18661 atgccactga gctggatgtg gacaaattgg attatgccac tgttataata aaaatcaaag
    18721 agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt tttgtatttg
    18781 acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat aaaatattac
    18841 acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt cttgatggta
    18901 ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca aaaatcgtac
    18961 aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga ttgaaaaaag
    19021 aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga tattactcgt
    19081 tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt gtttttcgag
    19141 attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa tgaagttgtg
    19201 gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta ttatgaggaa
    19261 tattttttag attctggaaa aacattagat cagatcattg atgaatatgg tacaccaaag
    19321 cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc agaaaacgag
    19381 ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat cggtttattt
    19441 gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg gggactattg
    19501 gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat cttaggtgtt
    19561 ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct gagtggatta
    19621 ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc gccactgttg
    19681 ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg gatgggacga
    19741 cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac acataaattg
    19801 agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg cctcctagca
    19861 ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg tcctcggttg
    19921 atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt catccatgca
    19981 gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac gacttatgat
    20041 aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa tgcagtcaaa
    20101 aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac tattgttgtc
    20161 cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc aattgatatc
    20221 aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt atcgttcaat
    20281 gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg attagatgag
    20341 gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct agaagacagt
    20401 ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt gcatgagatg
    20461 gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa agactaccag
    20521 ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac tgctaaagac
    20581 acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc ctgtactcaa
    20641 ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt ttcatcgctt
    20701 aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac agggtgaaag
    20761 agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg gaaagattac
    20821 atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa aaagtgatgg
    20881 ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat gaagttttca
    20941 aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt atcgatacag
    21001 gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt cgggaagatt
    21061 tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt acagaagaaa
    21121 agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg acacaggcaa
    21181 gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg gcgcgtattc
    21241 gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa ggataaagga gcgcaggaag
    21301 tagcatgaga gaaaaaagag cattttatat caatggttat gcagggattg tcggattggt
    21361 cgtattagct ttagttggat tgtttttctt ctatttaggt atgtggcaag cgaaagtttg
    21421 ggcacttttt ttaagtattt ttctttggtt gatcacgtta ttgttgttaa gttcggcaac
    21481 tgtagtcagt cctaatcaag caaaagtaat tttgtttttt ggacaatatt taggtacgat
    21541 tcgagaaaat ggctttttcc ttacgatacc tttggcacaa aaaatgactg tttcgttgaa
    21601 agttcggaat ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa accccatcga
    21661 aatctcagca gtagtagtct ttaaagtcgt tgatacagca aaggcattat tcgatgtagc
    21721 gtattatcaa gattttgtag aaatccaaag tgagacagcc attcggcata tcgctagcca
    21781 atacccgtat gatacattca atgagaatga tttgacgctt cgaggaaaca cgacggcagt
    21841 gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg tcgaagtaat
    21901 cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc gctagtgcca tgctccaacg
    21961 tcaacaagca aaagccattt tgtctgctcg acaaaccatt gttgaaggag ctgtcacaat
    22021 cactcaaatg gccttagagc aaatcgaaga tggacaagaa atcaatttca ccgatgatcg
    22081 gaaagtccaa ttgatcaata atttacttgt ctcaatcatt acagacaaag gaacgcaacc
    22141 tgttatcaat acaggcgaca tgaaagaccg taagtgacat actttttgta agataggaag
    22201 aaggagttta ggacggatta ttgtcttgaa ctcttttttt agttgtttta aagaaaatga
    22261 caaaattgaa agaagaatga tagttgattc gaaggcagaa ttgttcgctt ttttctataa
    22321 tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc ggtctacttg
    22381 ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt atgggtggag
    22441 aagaatacta tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa tcagactctg
    22501 gacaagaaac agtcaactat agatatatcc tgcctggcta tacgaaagag ggtaaggaaa
    22561 aagaactgga cttcaaggga caattgactc gaccgcttcg taaaggagca tatttaaaaa
    22621 tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa aaagacgtgc
    22681 cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca aaatagtcga
    22741 agtacagaat gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc aagcattaag
    22801 cggcatcagc ttcgatgtag aaaaaggcga atttattggg atcatgggcg cttctggttc
    22861 tggtaagtcg acacttttga atattttatc cacattagat aaaccaacag atgggcatat
    22921 ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat caactagcag attttcgtgc
    22981 gaatgagatt ggctttattt tccaagattt caatttatta gaaagcttga cagcacaaga
    23041 aaatatcgct gttcctcttt ctttacaagg cgtcagacca aaagaaatca agcaacgtgt
    23101 ccaaaaaatt gcagaacgtt tatccatctc tcatattttg gaaagctatc cttctgagat
    23161 ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac aaccaacgat
    23221 tctattaggg gatgagccta caggtgcgct ggattccaaa agtgcccggg atcttttaga
    23281 tacgatggac gaattgaata caaaagatca tgtatcgatt cttcttgtga cgcatgatcc
    23341 gttttctgca agttattgcc aacgtatttt attcatcaag gatggcggta tccatcaaga
    23401 agtgaaacgt ggagatcaaa gccgtgattc cttctatcga gaaatactat ctattcttgg
    23461 aaacttggaa cagtaggggg agattgacta tgctttggaa attatcactt actgggatca
    23521 agagccgttt aagagactac ttggtgttat tctcaggatt agttatggca tctgctattt
    23581 tttacatgtt tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat acgatcgttg
    23641 cggctacagt tatcattttc cgattcggtt ctgttttact agggatcatc acattcgttt
    23701 atattttata tgcaaattcc tttttgatga cgatgcggca aaaagactat gcaatgttta
    23761 tgatgctggg agcaaaaggc agaaaaatcg cacagatgat tttttctgaa acattcttag
    23821 tagggatcgc agctacttta gtgggttcag ctgtcggtgt aggattgaca agtatcgtcc
    23881 accgattatt agtggatcag ttagatctca atatttcgca ttttacacct tttagtattc
    23941 acgggttgtt gatcactgtt ttgttttttg ctgttttgtt tttattagct gctgttgtca
    24001 atgctttttc gattgtcaaa aaaccgattt tagcattgat tcgtgcggat cagacaccaa
    24061 caagaatcaa gcagcataaa ttcttgtttt tccttgaagt agtactggga attgcttttc
    24121 taggctgcgg ttactatatg atgaaacacg tggggaccta tcaagtcttt ggtatagggg
    24181 tagccttagt tacgatcgta ttaggaactt atttcatttt ccattctgtg atcattttct
    24241 ttttatcatt actaaagaaa tcagaacaga tttcattgaa aaaattgaac aatttcacat
    24301 tgtcacagtt aaactttcgt attcgtgaat atacacaaat gctctcaatg gtcgctatgc
    24361 tgtttgcctt ggcactgggc gctttgacgg taggattagg ttttaaaaat gaaattgcag
    24421 acttgacaga aaacaattct ggatatgatc tagtgatgaa taatcctcgg gcagaagatc
    24481 agcaaaaaat caaagaactt tcaccaacac tttctgcttc ttatactcaa aaagaagatg
    24541 cgggaacgat ttactataat gcaagtgaat ttgcggctca accattgatc atgataaaac
    24601 atgaagatgg aacaccgccg aagatcacga aagaaaaagt tcctgctaaa aaaatgaatg
    24661 aactgactgt acaagaggaa cttcgaagct atgaattgcc ggaacagcaa gaaaaagaaa
    24721 tcaaggtagt cagtcaatca gaatttgatc aactgaactt gccacaatcc actttacagc
    24781 tagttcaggt aaaagacttc caagcaaacc tgaaaccaat cgaaacactt gttgctcaaa
    24841 ataaaatgaa caatccatct ttgcaaaaag tagagtacag caaccagaaa tatgaaatgt
    24901 atgaagtgta taatggtcta ttctctggtt ttgaatttat gggattcttc ctaggtttag
    24961 ctttcttgac catgcttgct agctgtttga tgttcaagat cttgtcagga agcaagagtg
    25021 acattgctcg ttatgaaatg cttgaaaaga taggaacacg acgcgggttg cttaaacaat
    25081 cgatccgtag agaagtaggc gtgttattct tagcaccagg aatcttaggt gtagtccatg
    25141 tcttattcgg attacaaatg tttggtctat tcatgcaaaa tccatacaaa gatatttggg
    25201 ttccgtttac gatattcttc gtattgtatt tcatctacta tgtactgacg acatggttat
    25261 atactggaat cgtattgaaa aagagattgt gaaaaagctg tcctgcatca agtaataaga
    25321 acagccaagc aaaaatagct cctcatattt ttcgtttggt tgggcttatt accgcagatg
    25381 caagcttttg aacaccgttt gttcggaaaa agaggttgtg acaatatttt tgtcacaacc
    25441 tttttttcac ggtcttttat tagaatatag taaatttttt ctcgttactc acggaataag
    25501 ctgttaagaa aacggtttcc atgtatactg gaatcagtgc aaatgaaaga aaagagggtg
    25561 caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg attacgatca
    25621 cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt gttcattttc
    25681 aaacaataat cgataatgaa aaacgagagt tgatcttagg ttttgatgaa gcaaaagaat
    25741 atctagaaaa agacccttat atcggtgctt caatcggacg tatggctgga cggattacga
    25801 atggcacatt caaattagat gatgaaacgt tcgaagtaaa aacagacccg gacactggtc
    25861 attgtttaca tggtggtgca cctagctttg aagccaagca gtggcattat acagtcatca
    25921 atggtgaaaa cgaagcatcg gtcattttct atcagactag cccagatatg gaaaacggat
    25981 ttcctggaac tttagatgta gaagtacggt atacgctgac gaacgatgat acatggcgag
    26041 tgacggtcca agggatcagt gaccgcaaaa cagtattcaa tcctacaaat catgtctatt
    26101 tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca tatactttgg ttgaatagcc
    26161 gattatttgc tacgctcaat aaagatacaa cgcctacagg agaagtagtt tcagtaagtg
    26221 gcacagcatt tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc agtgattttc
    26281 gccaatcaat gatagtgggc ggcatcgatc atccattttt gttgaaaaaa caatctggtt
    26341 tactaaatgc tgcggaattg acaagcccag atgaaaaagt atcgatccaa gtaaaaacag
    26401 atgcttcagg aatcgtgatt tttacagcaa attttggaga agatggaccg gaaatgcgcg
    26461 ggaataaatt agtaaatcat ggcggtatca catttgagac tcaagaactg ccaggagcgg
    26521 agcgacttga aagttttgga gatgtcactc tggaacctaa ccaagtacgc gagtttgtaa
    26581 cggaatacaa aataaatata aagaaataga ataaattaaa aagggagtgt gacagtgtca
    26641 catccccttt ttaatttatt ctatttcttt aaaggcagat caagccagaa tgatttgtct
    26701 aaacttaaca aggttagtac aggaacagtt gcagctttaa ctgtacgagt agttgtttct
    26761 ggtatctgtc ttgccgtaaa attgttccac aaatcccaga ttgaagcgac acggaagatg
    26821 gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta atcctgtgat tggcacgcca
    26881 tctgttgtcc atttgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt
    26941 gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct
    27001 tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa
    27061 tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca
    27121 ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa
    27181 gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca
    27241 agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga
    27301 tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc
    27361 atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt
    27421 ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagccatc taatccatct
    27481 aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact
    27541 cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt
    27601 tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg
    27661 gaagaaggtc cccagaagac gaggtcgcca ggttgggcct gacttactgg aatctgcgta
    27721 cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat
    27781 acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat
    27841 ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt
    27901 gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga
    27961 ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca
    28021 ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat
    28081 ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt
    28141 gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc
    28201 ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc
    28261 ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt
    28321 gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca
    28381 gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt
    28441 tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc
    28501 ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct
    28561 gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt
    28621 ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc
    28681 gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc
    28741 gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt
    28801 ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag
    28861 acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg
    28921 tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt
    28981 tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta
    29041 tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat
    29101 cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta
    29161 actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta
    29221 cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag
    29281 gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta
    29341 cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg
    29401 caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac
    29461 cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag
    29521 ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga
    29581 tattactgat catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc
    29641 aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg
    29701 cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag
    29761 gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat
    29821 attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga
    29881 cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc
    29941 cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg
    30001 caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa
    30061 aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa
    30121 gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca
    30181 gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta
    30241 gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca
    30301 atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag
    30361 ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa
    30421 gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca
    30481 gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa
    30541 gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa
    30601 tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt
    30661 atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc
    30721 cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa
    30781 acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat
    30841 gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat
    30901 atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta
    30961 gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg
    31021 cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc
    31081 tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac
    31141 ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc
    31201 ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc
    31261 tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc
    31321 atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg
    31381 tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt
    31441 cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt
    31501 gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact
    31561 attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc
    31621 agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc
    31681 gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac
    31741 tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc
    31801 gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac
    31861 tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat
    31921 tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa
    31981 agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct
    32041 tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc
    32101 aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct
    32161 atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat
    32221 ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa
    32281 tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg
    32341 atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga
    32401 gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga
    32461 ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca
    32521 ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa
    32581 ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct
    32641 cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat
    32701 aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg
    32761 aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc
    32821 atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc
    32881 tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg
    32941 attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc
    33001 atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa
    33061 tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc
    33121 gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac
    33181 gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg
    33241 cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac
    33301 gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga
    33361 tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg
    33421 gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg
    33481 agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca
    33541 aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga
    33601 tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt
    33661 cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat
    33721 cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc
    33781 agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat
    33841 cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac
    33901 aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc
    33961 gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta
    34021 ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa
    34081 ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc
    34141 tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct
    34201 cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat
    34261 aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta
    34321 gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg
    34381 ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg
    34441 atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat
    34501 gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg
    34561 tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa
    34621 tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta
    34681 ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca
    34741 ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa
    34801 gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact
    34861 ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta
    34921 caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg
    34981 gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa
    35041 aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga
    35101 ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt
    35161 tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat
    35221 acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat
    35281 ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta
    35341 gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg
    35401 ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa
    35461 gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa
    35521 gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga
    35581 aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa
    35641 aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt
    35701 tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg
    35761 gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa
    35821 atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga
    35881 acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat
    35941 gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca
    36001 ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg
    36061 cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac
    36121 atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc
    36181 cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg
    36241 gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca atttggaccg
    36301 atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa
    36361 gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa
    36421 accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt
    36481 aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc
    36541 ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca
    36601 ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa
    36661 atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt
    36721 actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa
    36781 gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc
    36841 atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta
    36901 atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc
    36961 aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa
    37021 gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga
    37081 ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat
    37141 gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc
    37201 gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt
    37261 gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcggtt tgacctgagt
    37321 attagagaaa caaacggcaa aatagttttt catattttgc cgttttttga gctaataccg
    37381 aaggacagct gattgaacac catgaatcag aggttgtaaa aaagccgtcc tgcatcgagc
    37441 aaaaagaata ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc
    37501 gtagataaaa gcttttgaac accatttata gaagaaacaa gaatattttg ctattttaaa
    37561 caagaggata gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta
    37621 gaatgtataa agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac
    37681 ttgtatatat aagttgtggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca
    37741 tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat
    37801 ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc
    37861 gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc
    37921 tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata
    37981 gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg
    38041 acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc
    38101 attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca
    38161 tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac
    38221 tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa
    38281 ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa
    38341 aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat
    38401 ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa
    38461 atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta
    38521 tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct
    38581 actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg
    38641 agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc
    38701 aatcgatttg tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg
    38761 atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac
    38821 ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta
    38881 ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc
    38941 gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg
    39001 aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct
    39061 tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca
    39121 atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt
    39181 cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca
    39241 aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat
    39301 actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat
    39361 taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg
    39421 ttttatttaa aataatcttc ttcagaaaat gaagagtttt catactctcc tttatcgcca
    39481 taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact
    39541 aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca
    39601 agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga
    39661 atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc
    39721 ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca
    39781 atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat
    39841 ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc
    39901 ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta
    39961 cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa
    40021 aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa
    40081 aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata
    40141 tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt
    40201 ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg
    40261 atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc atatgcttcc aagccccaat
    40321 aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc
    40381 aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc
    40441 aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa
    40501 taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct
    40561 aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcactctttt tcctccttta
    40621 tctataactt cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca
    40681 aaaatatcgt caatcattca tataaacttt ctcatcaatt aaaataagaa aacgcttgac
    40741 agttctttta accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggtttg
    40801 cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa
    40861 acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc
    40921 aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat
    40981 gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc
    41041 agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac
    41101 agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac
    41161 attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg
    41221 aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata cacatgagct
    41281 agacccacat gtatggctag ctccaagttt agcaatcaag caggtagcaa acatacgtga
    41341 tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta
    41401 taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca
    41461 aagatctttt gtcacgcaac atgctgcatt caactatcta gcgttagaat atggattgaa
    41521 tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga gaattgctga
    41581 actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa
    41641 agatagtatt gccagaacgt tagcaaacga agcaggtgta tctttagaag tattgaatcc
    41701 tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga
    41761 agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgctag aagatacttt
    41821 gacccccaaa aaagaaaaga gcgtctacaa cggttatttt gaagacgagg atgtacaaga
    41881 tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtatg tagaagatgg
    41941 gacttttgat cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga
    42001 atataaagaa tattatacga agggctatca aacagatatt gcttcaatca agatcagtga
    42061 gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt
    42121 agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc
    42181 agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc
    42241 ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag tattgctgaa
    42301 agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca
    42361 ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt
    42421 tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga
    42481 ggctgggatg gtctcgccgt ttgccaacgt gacttctctg gtttttttat taatcgaccg
    42541 cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt
    42601 ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag
    42661 tttatgtggc gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt
    42721 gatctcatca ttaaactcta ttttgaagat atctttttgg atttgggaca tttgaaaatc
    42781 ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa gatcttcttt
    42841 gacgatataa tcagtcggtt cgatgttgct ttttaagatt ttcatcgaca tttccgtatg
    42901 ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc
    42961 tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga
    43021 agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata
    43081 actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg
    43141 ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg
    43201 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
    43261 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
    43321 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
    43381 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
    43441 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
    43501 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
    43561 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
    43621 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
    43681 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
    43741 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
    43801 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
    43861 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
    43921 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
    43981 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
    44041 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
    44101 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
    44161 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
    44221 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
    44281 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
    44341 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
    44401 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
    44461 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
    44521 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
    44581 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
    44641 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
    44701 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
    44761 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
    44821 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
    44881 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
    44941 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
    45001 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
    45061 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
    45121 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
    45181 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
    45241 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
    45301 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
    45361 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
    45421 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
    45481 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
    45541 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
    45601 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
    45661 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
    45721 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
    45781 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
    45841 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
    45901 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
    45961 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
    46021 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
    46081 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
    46141 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
    46201 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
    46261 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
    46321 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
    46381 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
    46441 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
    46501 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
    46561 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
    46621 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
    46681 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
    46741 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
    46801 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
    46861 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
    46921 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
    46981 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
    47041 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
    47101 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
    47161 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
    47221 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
    47281 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
    47341 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
    47401 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
    47461 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
    47521 aaaggaaaaa ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg
    47581 tctgttagta taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca
    47641 aattcaaaga agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga
    47701 ttacaaaaag aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg
    47761 ctacaagaag aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt
    47821 gaggaagaag tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag
    47881 aaacgttcct ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag
    47941 aaacatcttc gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa
    48001 gctgtttatc tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt
    48061 acaaggaacg ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc
    48121 gctgtccata tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga
    48181 aaatttcctg aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa
    48241 aaagtgaatg ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa
    48301 tatggagaaa aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta
    48361 gaagaaatgg ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag
    48421 ccgatatcag aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag
    48481 caattgctag agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag
    48541 atccagcatt atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact
    48601 aatccacttc aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt
    48661 gttgttggag atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc
    48721 aagaactttg aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct
    48781 cgggcagcta ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata
    48841 ccaaaggcga tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct
    48901 gatactaaag cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat
    48961 gggaaatata aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc
    49021 caatctctgc tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt
    49081 atctttggta tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca
    49141 ctgaatctgc aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac
    49201 tgctacctga aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatagcc
    49261 gttgcagatt atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga
    49321 gagtttgagg aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa
    49381 agctatcttc atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata
    49441 cgagaagaag taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc
    49501 caagcgacag ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat
    49561 gcactatgta tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg
    49621 attggttgta acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac
    49681 tttaatgaaa taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat
    49741 gttgcgatga ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca
    49801 agaaaattga aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag
    49861 tgatagaaga gagggtgtga cagaagtggt cagtttcaag aaagaagaag gaatgtacga
    49921 aaattgcttc tcaaattttt gtgaaatttc agcttatttt cgaagaagct atttctggaa
    49981 cacagtttat tcggaaaagg gaggtgtgac aagaattttg tcacacctcc cttatttatt
    50041 ttgaaaatga gaatactatt atcaaaatag agaatatata gaaaaattct cattattcaa
    50101 acgtaaataa tcagttattt tgattttttt tatattttat tagctaataa ttggtatttt
    50161 aaaagaaatt actgtagaat tggattataa ttaaaaagga gtgagaaaat gataaaagta
    50221 tatacttcgc ctagctgtac ttcctgtaga aaagcaagag catggttgaa agcgaatcaa
    50281 cttgaatttg aagagaagaa tatttttgcc gaaccgttaa cagaaagtga gatcaaaaag
    50341 atcttggcag cgactgaagg aggagttcaa gatattattt ccactcggtc gaaaatttac
    50401 gaaaagctgg gcatagattt taacgaatta actttaaagc aaatgatcgt cttattcaaa
    50461 gaatatcctt cattattgag acgacctatt ttgattgatg aaaaaagaat actaatcgga
    50521 tttaatgaag atgagatccg gtcatttgtc cctcgtgaaa ttcgatgtgt tgccagaaat
    50581 ctgatctatt tacaaaacaa taacatctta aattcatagg gagggagcct tcgtggaagt
    50641 gaaagtctat ggatcagcgc actgtgtaac aagtctgaag gcgaaacaat ggttagaaca
    50701 ccatcaattg ccttatcaat ttgttgactt agaagaacgc gatttaagta cagaagaagc
    50761 agaagaatta tgcagttttg aagaagtgca agcagaacag ctatttgcga cttggtctga
    50821 ggcttttcac aacattgcga ttgacctttc ctgtgtagga aaaaaacaac tgattttttt
    50881 atgcacacaa cataccaatc tcctcagacg tcctttgatt attatcgatg aagttctatt
    50941 tattggatac aatgagcagt tgatggaaca gcttatctta aataaataga ctttaactgt
    51001 tttaatcaag tcactgagca tttcatacag ttgtaacagt cgaacactta gcatcaagcg
    51061 tgttatgtgt tctttttttg actttatttt tctactatag agacatgctc aatttatgtg
    51121 gtcaaaattc aaatctaggt ggggatatag agttaaaaat atattgacat gtgtaaattt
    51181 acatgatata ttcatttctg ttgttttaag tagcaaagca ataaaatagt attttaaaaa
    51241 acatataaaa tagttgttga catgttagaa atgacatgat aagataactg agttgttaaa
    51301 aaagaaccac ttgtgaagct tgattctatc gaaaaaaact tcaaaaaagt tcttgacaaa
    51361 taacaaatga taagttatga taaatgagtt gctgtaagat ttcgaaaaac gaaatcaaaa
    51421 aaacttttaa aaaagagttg acaattactt gtctctctga tatgatataa aagttgctac
    51481 ggcagccaaa aacgaatcgg ggaacactcc tgatgaagta gacctttgaa aactgaacaa
    51541 agtaagacaa accaaatgtg tagggcgtct tgattcaatt caagacaaca aaca
//
LOCUS       DAZUJO010000012        49421 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000012 DAZUJO010000000
VERSION     DAZUJO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.012"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..316)
                     /locus_tag="ACG8OL_000780"
     CDS             complement(62..316)
                     /locus_tag="ACG8OL_000780"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4816787.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(720..1502)
                     /locus_tag="ACG8OL_000781"
     CDS             complement(720..1502)
                     /locus_tag="ACG8OL_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816788.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1567..2403)
                     /locus_tag="ACG8OL_000782"
     CDS             complement(1567..2403)
                     /locus_tag="ACG8OL_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4816789.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2421..3830)
                     /locus_tag="ACG8OL_000783"
     CDS             complement(2421..3830)
                     /locus_tag="ACG8OL_000783"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4816790.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3844..5667)
                     /locus_tag="ACG8OL_000784"
     CDS             complement(3844..5667)
                     /locus_tag="ACG8OL_000784"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4816791.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6510..6836)
                     /locus_tag="ACG8OL_000785"
     CDS             complement(6510..6836)
                     /locus_tag="ACG8OL_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816792.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6882..7244)
                     /locus_tag="ACG8OL_000786"
     CDS             complement(6882..7244)
                     /locus_tag="ACG8OL_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS4816793.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7281..7495)
                     /locus_tag="ACG8OL_000787"
                     /pseudo
     CDS             complement(7281..7495)
                     /locus_tag="ACG8OL_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7623..7958
                     /locus_tag="ACG8OL_000788"
     CDS             7623..7958
                     /locus_tag="ACG8OL_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4816794.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(8034..10031)
                     /locus_tag="ACG8OL_000789"
     CDS             complement(8034..10031)
                     /locus_tag="ACG8OL_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816795.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10129..10404)
                     /locus_tag="ACG8OL_000790"
     CDS             complement(10129..10404)
                     /locus_tag="ACG8OL_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816796.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10636..11370
                     /locus_tag="ACG8OL_000791"
     CDS             10636..11370
                     /locus_tag="ACG8OL_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4816797.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11409..12551
                     /locus_tag="ACG8OL_000792"
     CDS             11409..12551
                     /locus_tag="ACG8OL_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4816798.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12646..14037)
                     /gene="sufB"
                     /locus_tag="ACG8OL_000793"
     CDS             complement(12646..14037)
                     /gene="sufB"
                     /locus_tag="ACG8OL_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS4816799.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14042..14512)
                     /gene="sufU"
                     /locus_tag="ACG8OL_000794"
     CDS             complement(14042..14512)
                     /gene="sufU"
                     /locus_tag="ACG8OL_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS4816800.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14499..15734)
                     /locus_tag="ACG8OL_000795"
     CDS             complement(14499..15734)
                     /locus_tag="ACG8OL_000795"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS4816801.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15731..17017)
                     /gene="sufD"
                     /locus_tag="ACG8OL_000796"
     CDS             complement(15731..17017)
                     /gene="sufD"
                     /locus_tag="ACG8OL_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS4816802.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(17030..17803)
                     /gene="sufC"
                     /locus_tag="ACG8OL_000797"
     CDS             complement(17030..17803)
                     /gene="sufC"
                     /locus_tag="ACG8OL_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS4816803.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18000..18848)
                     /locus_tag="ACG8OL_000798"
     CDS             complement(18000..18848)
                     /locus_tag="ACG8OL_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4816804.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18872..19558)
                     /locus_tag="ACG8OL_000799"
     CDS             complement(18872..19558)
                     /locus_tag="ACG8OL_000799"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4816805.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19551..20585)
                     /locus_tag="ACG8OL_000800"
     CDS             complement(19551..20585)
                     /locus_tag="ACG8OL_000800"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4816806.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20648..20904)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20980..21318)
                     /locus_tag="ACG8OL_000801"
     CDS             complement(20980..21318)
                     /locus_tag="ACG8OL_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS4816807.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21320..21676)
                     /locus_tag="ACG8OL_000802"
     CDS             complement(21320..21676)
                     /locus_tag="ACG8OL_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS4816808.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21682..22869)
                     /locus_tag="ACG8OL_000803"
     CDS             complement(21682..22869)
                     /locus_tag="ACG8OL_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS4816809.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(23060..23905)
                     /locus_tag="ACG8OL_000804"
     CDS             complement(23060..23905)
                     /locus_tag="ACG8OL_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4816810.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            24139..25104
                     /locus_tag="ACG8OL_000805"
     CDS             24139..25104
                     /locus_tag="ACG8OL_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS4816811.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25481..26524
                     /locus_tag="ACG8OL_000806"
     CDS             25481..26524
                     /locus_tag="ACG8OL_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS4816812.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26586..27050)
                     /locus_tag="ACG8OL_000807"
     CDS             complement(26586..27050)
                     /locus_tag="ACG8OL_000807"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS4816813.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27296..28243
                     /locus_tag="ACG8OL_000808"
     CDS             27296..28243
                     /locus_tag="ACG8OL_000808"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4816814.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28454..30526)
                     /locus_tag="ACG8OL_000809"
     CDS             complement(28454..30526)
                     /locus_tag="ACG8OL_000809"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4816815.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30459..30836)
                     /locus_tag="ACG8OL_000810"
     CDS             complement(30459..30836)
                     /locus_tag="ACG8OL_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS4816816.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30965..31525)
                     /locus_tag="ACG8OL_000811"
     CDS             complement(30965..31525)
                     /locus_tag="ACG8OL_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4816817.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31675..32967
                     /locus_tag="ACG8OL_000812"
     CDS             31675..32967
                     /locus_tag="ACG8OL_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4816818.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(33027..34145)
                     /locus_tag="ACG8OL_000813"
     CDS             complement(33027..34145)
                     /locus_tag="ACG8OL_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS4816819.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(34159..35580)
                     /gene="arcD"
                     /locus_tag="ACG8OL_000814"
     CDS             complement(34159..35580)
                     /gene="arcD"
                     /locus_tag="ACG8OL_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS4816820.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35826..36947)
                     /gene="mnmA"
                     /locus_tag="ACG8OL_000815"
     CDS             complement(35826..36947)
                     /gene="mnmA"
                     /locus_tag="ACG8OL_000815"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS4816821.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37351..37950
                     /locus_tag="ACG8OL_000816"
     CDS             37351..37950
                     /locus_tag="ACG8OL_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4816822.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(38088..39374)
                     /gene="efmA"
                     /locus_tag="ACG8OL_000817"
     CDS             complement(38088..39374)
                     /gene="efmA"
                     /locus_tag="ACG8OL_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS4816823.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39645..39989)
                     /locus_tag="ACG8OL_000818"
     CDS             complement(39645..39989)
                     /locus_tag="ACG8OL_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS4816824.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(40126..41271)
                     /locus_tag="ACG8OL_000819"
     CDS             complement(40126..41271)
                     /locus_tag="ACG8OL_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4816825.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(41451..42425)
                     /locus_tag="ACG8OL_000820"
     CDS             complement(41451..42425)
                     /locus_tag="ACG8OL_000820"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4816826.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(42505..43380)
                     /locus_tag="ACG8OL_000821"
     CDS             complement(42505..43380)
                     /locus_tag="ACG8OL_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS4816827.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            43510..45417
                     /locus_tag="ACG8OL_000822"
     CDS             43510..45417
                     /locus_tag="ACG8OL_000822"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS4816828.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(45958..49236)
                     /locus_tag="ACG8OL_000823"
     CDS             complement(45958..49236)
                     /locus_tag="ACG8OL_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS4816829.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT        14201 a        10380 c         8706 g        16134 t
ORIGIN      
        1 cgacataaca aagaaacagt gtaatcctta caccattttt caataaattg aacaagcgaa
       61 tttaggtttt ccgagagaat atggctgcgg ctttttttaa gatttcaagt tcctcattta
      121 aacgagcatt ttcttttctc agttcagcta cttcttttcc agtcagtcct gtggactgat
      181 caggtaaata taaattcttc catttgtaaa tcgttgcttc agataagcca tattctttcg
      241 ctaaccctcg aacagaacgt ccagtttgat tcagttcaac aatcatttgt ttaaaatttt
      301 cttcatatcg ggtcataaaa attcatcctc tcgtagataa gtttaattta ttcttgtctc
      361 tacgaaaagc tgtctaagat ttgatactaa caccaagtta tctttagaac catttcagat
      421 acatatgaaa caattacata tgattttttc ataaaaaaac agtactgtct agtagtataa
      481 aatatacaaa tttcttaaaa attttttcac taaaatcact acctgaaaca gtctaaaaca
      541 cttatatatc aacgtttaat gaaactatct ccgtttcatt tcagatacaa ataaatatag
      601 tcgggctcca aatccgaaaa aaaatacccc cagtagctta tcctaagcta ctggagatgt
      661 ataagtacgg tcatataaca acggggtagg gtaaataaat gaaaaagatg gaagaatcat
      721 tattcaataa cttttttaaa tacttcgttg taatcagtta cctgtccgtt cttaaatacc
      781 acagaataat cttttttccc attttcaagt aaccgaagtg gaatgtcttt accaacttgc
      841 tctgtcactg cattactgat acctgttaga ttagctaata aatctgataa agattgagca
      901 tctgtagaaa tctctgtatc agtatttatc gtgattcctt tatcttcatt tacaagcttc
      961 caagtaggtg gtatagctgt gtatgtttcg atgaatgaat caattctttc atctatcgtc
     1021 ttcccagtat ctacttctgt ctcattactc ttagatgaat cgattctttc atccatcgtc
     1081 tgctccgtaa ctgcttctgt ctcattattc tcaccggaaa ctctcttcac acccaaactt
     1141 atatattgtc catctttagt gtgcatagtt aaaattccca cgcttttact aataaattca
     1201 ctataaccag ttctttcaat agagacttca atgtatttca cactatcata ctcaaaagaa
     1261 aaatagacat gtaaaagcat tttgttgttt acatctacat aataatcctt tgactctact
     1321 tgagaccttc ctatacggaa tacagcttta ctgtctgata tctctatacc agcatcctta
     1381 ttattaatct gccaatctcc aagtagctct tctggcaagc tagtagtttc ttgtgaacac
     1441 tttaccactt cactggtaga ggtcgaaaca ttcacctccg cactgttaga agaattttcc
     1501 aaggaatcag gagccttagc acaaccaaca agtctttata tattgtgcag ccgatatttt
     1561 cttcattcag tacacccttg aaatatgcag tgttagataa agcagttcgt cctgatttgc
     1621 tgaggcaccc atctcttctt gaacgtactc cttgattttc aaggccgccc gatactcttt
     1681 cggaaaacgc tgcttcactg aagccagcaa aaaatcatcc ttctccggca actcttcctt
     1741 cttaatcatt cgggcaataa aaaactgtaa atgcgtcaca aatcttaagt aaagcatcga
     1801 atctttatca accttttttt cgggaaacag atatcctagc atagccaaga tatcctttag
     1861 tgctcttacc gagagcatgg tttcttccat atttgcttgt tcactttggg catttacaat
     1921 gtgaaacgca atgtttccag cctcctcttc tggcaactga acattcattt ttttgttgat
     1981 caattggata gcatagcagc cgatctcaaa ttcttttggg taatactttt gaatttcaat
     2041 cagcaggcga ttttgaaaaa caatgccttt ttgactgcgt tcaatggcat gcttcaagtg
     2101 atcaaccaag gtaaaaaata gacttttatt cagcttaaag tcaaacttgg cgttggcata
     2161 atcgataatt cgcttgacgc aaataattag atcatccgga gcagcctcaa acagtttaat
     2221 gtaatccttt tttagagcat cagaaagtga atctaattta aatacttttt ggattttgtc
     2281 agccggaacc aaatcgcctt tctttagttg aaatccaatc cctttcccca tcaaaattac
     2341 ttctttgcct tgcttatcta agcaaagaat taaactgttg tttaacgttt taattactct
     2401 cattagattc accgttctct ctaagctaag ccatttcttg cgattacttt ttgataccaa
     2461 aagaagcttt ctttttttat tcgttttaat tctttaagat tctcatcggt tcggtcaaca
     2521 tatataaaac cataacgctt attgaagcct tgatgggtac taacaacatc aatcgctgac
     2581 caaggtaaat aaccaaaaac atctacacca tctgttaaag ctgcttgaat agccagaata
     2641 tgtttggcta gataatcaat tcgatcctgg tcattaattc gtctatcttc ctcaactatg
     2701 tcgggtgccc catagccatt ttcagtaatt aaaataggta aatcatagcg atcgtacaat
     2761 ttcctcaatg ttaatcgtag tccagtaggg tcaactaccc agccatatgg cgttttatct
     2821 acataaggat tttcagctgc ccggtagaca ccttcttctc ccaacatgat ctgttgatcc
     2881 cccgctctag cggaaacatc tgatccgtca tttttgctgg cagcgatggt ggcagttgaa
     2941 taataattaa tcgcaataaa atcgggttta ccggaggata aaattgcctg atcctctgcc
     3001 tcaattttag gatataatcc acgatcattc aaatagtttt caaacaaata attgtatttt
     3061 ccccgaactg ctacatctaa gaaagaccag cctctcaatg tttcccaatt atgggctgca
     3121 attgcatctt ctggactgga ggtagcttga tacatagccg ttaaattgat tgctggaccg
     3181 atttttcctt taagattcaa ctgatgatac aagtgaatta ctctagcttg agctaataaa
     3241 acatggtgat taatttgaaa gagttctttt tttgaaggca gctctccact tggtggcaaa
     3301 ccaatcgctc caggatgaag gatcatcgtg ttttgctcat taattgtcag ccaatagttc
     3361 accttgtcac catactcagt aagcaatacc tgtacatagt ccacaaaata atcgatagtt
     3421 gctcgattta accaaccgcc ctgctcctct agaaaataag gtaaatcgaa atggtacatg
     3481 gtgacaatcg gttcgattcc ctgattcagg cattcatcaa ttacttgatg ataaaaagaa
     3541 attccctttt gattggtttt attattttca atatccataa ttcgcgtcca agcgatggaa
     3601 aagcgaaaag cttttaagcc taattcttta aacagctgta tatcttcttt atagtgatgg
     3661 taaaaatccg aggcaacttc aaaattagct accccttctg tatgctgata cttgtcaata
     3721 atagacggtt gtttcccatc ctcctgatag gcaccctctg cctggtaggc actaatggag
     3781 gcaccccata aaaacttctc tggaaatgct gaaagctgtt tgtattgcac aaaaatcctc
     3841 tccttaacta atttgcatca atggttcgtg ttcctttaag cttccgcttg cttcactaat
     3901 tcctgcgtaa ttttcactgt tggtcactac cacaatcact gtatcatcgt agccagcgtt
     3961 tctaatggca gcgaaatcgg cgttgattaa taattgtcct gctgacacct gatctcctgc
     4021 ttttacaaat acttcaaagg gttccccttt taaattcacg gtgtcgatcc ccacatggat
     4081 caataattca ataccttctt tagaacgtag accaatcgca tgtttagatt ctaaaatagt
     4141 aatgacttct ccctcaacag gtgaataaat gctgttttct tctggatgaa tggctacgcc
     4201 ctttcccaat agtcccttag aaaagacagc atcgttcact tcagaaatag gacacacggt
     4261 tccttttacc ggattttcaa caatgactgc ttcggctatt gccgtttgct ctgattcatc
     4321 agaagtatcg tcaaatccaa ttagataagc aatgataaag gttgcaacaa aggcgattac
     4381 acacgtaata attgcgtgaa caatattcat tggattttct ccaataaagg ctggtaaggc
     4441 tgctaagcca ggggaaacaa aggcatatct gactaaaccg ctcaagccag catacaaacc
     4501 agcaattgca ccaccaatca tggaagcaac taatggtttt ttcagcttca aggttactcc
     4561 atagagtgaa ggctcagtaa ttcccattaa agcagtgatc ccagtggaca cagctagctg
     4621 cttcaactct ttgtttttag ttttcgcggc tacagcaaag gtagcacctg attgagcaat
     4681 attagaagcc agcattcctg gcccattgat catttcagaa cccgtactgc tcaattgcat
     4741 agtagcgatt ggcgtcattg cccaagctgt acccgtaacc accaaaaatg gctgcaaacc
     4801 acccatcaaa aatggaatta accaagctgc tttgccgtta atccattcag cccccattgc
     4861 tataagatcg ttcagcaacg ttccaaacgg tccaatgaca accaacgcta aaggccccat
     4921 aattaaaaat accagcatcg gcttagtaaa gaatttaata aacgaaggcg aaattcgatc
     4981 cgcaaagggc tctacatagg acatgatcca aacagtaaag ataatcggta aaactgatcc
     5041 cgcataatct gcagctaata ccggaattcc tagaaaagaa acggcatcgc cagtagaaaa
     5101 aagctgtgaa atatttggat gaagcaaaat gccagcaaca gtaatcgcca aaattgggtt
     5161 gacatcaaac tttatggctg ccccataggc taatagtact ggcataaaat aaaaggctgc
     5221 atcagcaata gtattcaaaa tgatgtaagt ttgactactg tctgatagta caccagtcaa
     5281 agtcaagacg gccaaaatgg cttttaccat cccggcacct gttatagctg gaatcatcgg
     5341 tgtaaaggtg gtagaaatga cactaatcaa tcttgaaata atactttctt gattgtctga
     5401 agaggggccc tttttaattg aagttcccac ttccttttga atttccttga aggctgtttg
     5461 gacttcattg ccaatcacca cttggtattg tccaccgcta tccaccacac taattacacc
     5521 aagtgttttt tctaaggctt tcgtatcggc tttcctttta tctttgagtt caaaacgcag
     5581 tctggtggca caatgggtta agctgaccac attttctttt cccccaacct gttgcaatac
     5641 atttcttcct acttctctgt aatccattat tctctctcct taaagcagta aaaaagacca
     5701 agtcaaacca cgccccaatc aacattgaag tgttagtttg tcttggtctt gcctgcataa
     5761 tcagtaacat gccaataacg ttattttgct gtaatccaat aatagcacat tatgatagcg
     5821 caatcaacag ggaatttaat tttttatctt ttcgtttgac cgtatagggt aatataacag
     5881 ttagatcaca tcaactaggg tatatctgcg tatacttact gctaaaataa gtatatctaa
     5941 tattaactat accagtaaaa ccattcctac ataaccagcg ggaaattccc aaaatcgtga
     6001 aagcatcaaa aatgcatata acggatcaca caacttcaac aattctgctc ttgggaaaaa
     6061 ttgatgaatt tggtgagtaa aggatggaat aaacagcatt ccaacaatat aggcaacaat
     6121 agtgatactt gtttttttgg taaaaatcag taagcaatag ccaatttcaa tcattccgag
     6181 atataaaggt atcgatagaa tgatttgggc tagtattctt aaaaattaac tagcaaatgt
     6241 ttaaactgat ttatttctaa tctacatgta gtaagtattt caattccacc aattatcaat
     6301 aaaaatacca aacatatcca taataatttt ttctttttca tttcttttat attgttataa
     6361 cagtgaataa cataaaaatt cccgacaatc attgtaatat ttttttgtca cctgtattag
     6421 tatttaattt ataggaaact ataaaaactc cctagtgaat ttacaccttt gggagttcct
     6481 gtttggacgg tatgcataat acatttaaat caatttaaca ttaacacctc agcatatggt
     6541 atctccacat atatacggtc tttctttgga tagtactcaa tatttatgtt gatagcactt
     6601 ccattgtcta atatagcttt agcccggtat gtgttatctc cattttcatg agtaatcgtc
     6661 acatcatcac atgatcgatt taagttgtac tgttcttgta aaatctgtgt taccaatggt
     6721 ttcgatgact tctctaatcc agatgtacct gtcatttttg aaaaaatgat acctataata
     6781 gccaagacaa cgataactat aatgataaac ttcttgttca tatttacttt attcaacttt
     6841 ttatctccta tctccaaaat aatttgctct tatccctctt atcagtacat tgacatccct
     6901 aaaagtacac caaagattgc tccagctacc cctgctatca tcaaaatcgt acccatttgt
     6961 tcatctttat ctcgatataa gtagcccata ataacagcaa cagcaccaaa tacaacagga
     7021 ataaatgcta acgctactac catagaaatc caaccaataa tcaaatacag cttccctgtc
     7081 tcttgtccat gtacgatttg ttccttcatt tcattaattg tctgctcagt acttttcttt
     7141 actgcgtctg ctccagttgt ctcatctgtt tcttgtagtg ctccgcaaaa ctggcaatac
     7201 ttactttcat ctgttatttc tttgccacat tttctacaat acatgtcttc tcctcctaaa
     7261 aacattcaat tgtttattag ttaactgttt actttattaa atacatcaga aaacagctca
     7321 ttcattggta cacttaatat ctcagaaagc atcttcattt gatagggttt aggaatcgtt
     7381 ctccctgttt gccacgaact tacacattgc tgtgtaacat tcattttttt gctaactccg
     7441 tttgagaaag tcctttttct tttctgtatc ggctgagatt acttactttt ttcatgattc
     7501 tcccctcctg ttttattata tacaacaata tgatgtattt caactcataa aatataaaaa
     7561 aacttcaaat accattacaa atacaacaac ttgttgtact atactgagat gggggtgtat
     7621 taatgaaaca gaaacaatat gcaaaggtca ttagagaaaa gcgaaaagct ctcgggtgga
     7681 cacaaaaaga attagctgaa aaaatcttct ctacacaaca ggcagtagca agatgggaaa
     7741 attctgtaac agagcctaac ctcgatagct taaccgctct ttctagagcc ctaggaaccc
     7801 ctgtaagcca ttttctagac aatgttgtcg tagattatga ggaagagttt ttggctctct
     7861 atcgttctct tagcactgaa gatgcagttc gaacaattga ctatatgaag ctattaaaaa
     7921 ggcaagaaaa tgaacgcaat cagctattga aagattgaaa aataaataac ccaccgaact
     7981 ttaacggtgg gtgtattgag atgaaataag attaagagct atggataacc tgtctagttt
     8041 aatacaattg ggtcttttct cttccttact ctccgtccat catctcttgt tacacttgta
     8101 aattgataaa gaggaactaa ttcaactcct gataatatca attcttttat tacttcatct
     8161 atccctgctc cttttgctag gtagactaaa aaactactac ttgctttcac attgtatagt
     8221 ctcttaatat ccttagaagc tcgttcaaga gtgaatcctt gtttaacaaa gtagcctctg
     8281 ttttctttta aagcattttc cacgctgtta ttcccaacat tacgtagacg ttcacaaaca
     8341 gtgttagtaa gttctttctc tgtcattaga tatgttcctt gccacgataa gccctgtaaa
     8401 acttcatgtg cttcatctaa aatcaattgt ttttccttgc taagttctga tcgggaattt
     8461 tgaggaaata ctcttttagc ctctttttcg ttaaaacata tcttaaccca ttcataagaa
     8521 aaatattgat atgtaaaatg aaatttttcc agaagtggaa ggatatcttc attcaatttt
     8581 tgaatcacag aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgatta
     8641 aagtttccta acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga
     8701 acatcttcat catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta
     8761 ttacatttat ctatgtcata agttttacca tagtaaaaac tcattttttt agctaattgt
     8821 tcccccgaca aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa
     8881 agcaagacat catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt
     8941 ttgtctttca ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga
     9001 aaatgtgtag gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg
     9061 tctttcttta aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg
     9121 gaatgacaat aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta
     9181 tgatcgttgt gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca
     9241 atggttaaca atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag
     9301 tctttaaacc actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg
     9361 gtttcattga ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc
     9421 atggtaaaat caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc
     9481 actgcatctg acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca
     9541 cacccgggga tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta
     9601 attgcttctt ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata
     9661 cttttttgta tcgcaaacgc agaattatga actaaattga tagataaaac cttttctatg
     9721 cttaagtatc tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca
     9781 gtgttatcga catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct
     9841 ttattctttg tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca
     9901 tctcttgtaa cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt
     9961 tttgtaactg gcttctctaa taatttttct ttatctacat aaattggaat attaatcatt
    10021 gatgtttcca tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata
    10081 ataaaaacaa actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata
    10141 ccgttccatg aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat
    10201 aacaggtaac ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata
    10261 ttcacttgcc tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag
    10321 cataggcttt agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat
    10381 gctatcaatg atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt
    10441 tctaagaatt atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta
    10501 atcaaacaaa aaatgattac cagttcatcg acatacacat aatacctcga taaaaaagag
    10561 gtgtcaatat ctttgtaaaa tttatttaca acaccccttt ttcgaggtat actaatttta
    10621 ggaggtgtgg tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata
    10681 ctcggaacaa aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt
    10741 cttatgatta acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga
    10801 acctatttac caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata
    10861 gaggagttag tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat
    10921 agcattgtac tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa
    10981 cagttcccac cagtacactc taatttagat acatggctgg atagatattc caaattagaa
    11041 ccagagatgc aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt
    11101 agtcttttca atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt
    11161 gacaataaca ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat
    11221 gaatggctac ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt
    11281 actgataata taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag
    11341 aaaaaaatga agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag
    11401 atgatttcat ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt
    11461 ataaaggaca cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag
    11521 gttttagtag caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat
    11581 acggtgttaa aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa
    11641 ctcatcagcg taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata
    11701 atcaaatact tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc
    11761 aaaaagtggt agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga
    11821 ctgcacaaat tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa
    11881 aaacacttct tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg
    11941 agcttaataa tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct
    12001 tcagattatt ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag
    12061 acatagacac gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg
    12121 gtaaagttat aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata
    12181 ctgagactct ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac
    12241 ttggatacaa tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat
    12301 acattccaaa cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc
    12361 gtattacacc tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat
    12421 ctgtcaaggt agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct
    12481 atgcacatat tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg
    12541 ctcatcagta aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa
    12601 agctagaacc cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc
    12661 ttccatctca taactgatca atcgattgag ttcgactgca tattccattg gaagttcttt
    12721 ggtgaatggt tcaacaaagc ccatgacgat catttcagtt gcttcagatt cggtcaggcc
    12781 tcggctcatc agatagtaga gttgttcttc tgagattttt gagactttgg cttcgtgttc
    12841 taaagagact tggctgttgt ggatctcatt gaatgggatc gtatcggatt tcgaccattc
    12901 gtccatgatg atcgtgtcac attcgatatg agagatcgaa cccgcactgt ttttggcaaa
    12961 ggttacttgt cctcgatagt ttacttctcc gccgtctttt gaaattgatt tagagacgat
    13021 agagctagat gtatttggtg cattgtggat catttttgca cccgtatctt ggatttggtt
    13081 tgctcctgca aaggcgatgg agagcatggt gcctcgtgca ccttttccat ctaagtaaac
    13141 acttgggtat ttcatcgttg tttttgcacc taggtttccg tcgatccatt caacggttgc
    13201 gccttcgtat gcttttgctc gtttggttac taagttgtac acattgtctg accagttttg
    13261 gatcgttgtg taacggcaat acgcatcttt tcttgtaaag atttctacga tggcggcatg
    13321 caggctgtta cttgaataag taggtgccgt acaaccttcg acatagtgga tgcttgctcc
    13381 ttcgtcaaca atgatcagcg tacgttcgaa ttgccccgtg ttttccgcat tgatccggaa
    13441 gtacgtttgt aatggaacat cgactttgac gccttttggc acgtaaataa aggtcccacc
    13501 tgaccatacg gctgaattca gcgctgctaa tttgttgtcg gttggcggta caagttttga
    13561 aaagtactct ttgaataatt cagggtattc tttcaaagca gagtcagtgt cagtaaagac
    13621 gattcctaac ttttcgaatt catctttcat attatggtac acgacttctg attcatattg
    13681 tgcggaagca ccggctagat aagctcgttc agcttctggt ataccaatct tttcaaatgt
    13741 atctttgatt ttttctggta cgtcttccca gtcacgagcg ggtttgtcac ttggtttttg
    13801 gtaatatttg atggcatcga aatcgatgtc tgaaaggtcg gggccccatg tttgcattgg
    13861 cattttgtta aatgtttcta gagatttcag acggaattca agcatccatt ctggttcgcc
    13921 tttgattctt gacatctcac ggatcacttc ttctgtcaag ccttctcctg tactgaaaac
    13981 cggttcgaca tcatcgtgaa acccgaattt atattcttct aattctggta cgcccatgtt
    14041 ctcactcctc ttccttgtgg taatgatgga aagctgcggt accatcgttt tccattgctt
    14101 ttcctagtgc tttccaggca agtgtcgcac atttgatccg agcgggaaat ttagctacac
    14161 caccaagcat agcagcatcg cctaattggt cgggatcttt gacttctttt ccttggacaa
    14221 gctgtagaaa gtctacagtt aaatgttctg cttcttctat tgattttccg atgacggcgt
    14281 ctgtcatcat cgaggcactt gccgtactga tggagcagcc actgccgtca aaggcgatat
    14341 ttttgatgat tccgtctttt acatcgactt gcaattcgat cacatctcca catgtggggt
    14401 tgttcaattc gatcttgctt gtcgagtcag tcaagcttcc acgatgatgc ggatgggaag
    14461 aatgatccaa gatcacttga cggtataaat tgtctaattt agatagtccc atgttggaaa
    14521 aactcctttg ttgccatgat tgcttcaact aaacggtcgg cgtcttgcgt tgtgttgtag
    14581 aaataaaagc tcgcgcgagc agtcgctgat acttcaaggt acttcaatag cggttgcgca
    14641 caatgatgac ctgctctgac ggctacccct tccatgtcta aagctgtcgc gacatcatgg
    14701 ggatgtaacc catccaaatt gaatgcaacg actcctgtcc gaaaattcgg gtcttgtgga
    14761 ccataaacag tcaatccatc aattgcttgt aattttggca acacataagc ggctagttct
    14821 tcttcatatc gatggatatt gtccataccg agtttttgca aataatcgat tgcatggcct
    14881 aatgcgatag cgccggcgat atttggtgtc cctgcttcga atttccaagg tagttctttc
    14941 catgtactgt cgtataattc gacaaaatcg atcatttcgc caccaaattc aacaggttcc
    15001 atttcttcta accattttcg tttaccatat aaaacaccaa tacctgttgg tccgcacatt
    15061 ttgtgtccgc tgaaagcaaa gaaatcacag tctagtgctt ggacatcaat ggccatgtgc
    15121 ggggcagatt gagcaccatc tactacgatg accccgccgt tttcatggac aagttcagct
    15181 aattcttgaa caggattgaa gacacctaag acattggata catgagcaat ggagacgatt
    15241 ttggttttgt ccgtaatctt tttcgctgca tcctccatat ccaggaagcc ttcttctgtt
    15301 agttcgatat atttcaagac agcacctgtt cgttttgcca actgctgcca cgggataaca
    15361 ttagaatgat gttccatgta agagagcact atctcgtctc ctgctttgat atagcggtcg
    15421 ccgaaggttc tggctaccca attcaagctt gtcgtcgtac cacgagtaaa cagcacttct
    15481 gctgtttctt ttgcatggat gaacgcccgt actttttctc ttgcttgttc ataatcatgg
    15541 gtcgcgcgtt ctgctaacgt atggacaccg cgatgaacat tggcgttatc gtgttcgtaa
    15601 tagtacgcta atgcctggag tacttgtttt ggcttttggg tcgtcgctgc attatccaga
    15661 taaactaaag gttcatcatt gacgtcttga aataagatag gaaaatcatt gcgtagcttt
    15721 ttagcatcga tcatccattc aacttccctt cgattacttc gactaattct ttccgaactt
    15781 gttctacagg gattgctgtt aagacagaac cgaggaatcc tcgaatcact aagcgctctg
    15841 cttcttcttt atgcaatcct ctactcatca gatagtacat ttcttctgga tcaactcgtc
    15901 ctacactggc agcatgtcca gcagttactt cgttttcatc gatcaaaaga ataggattcg
    15961 catcaccacg tgctttatca gaaagcatca agacacgact ttcttgttgg gcatctgcac
    16021 cttttgcccc tttcaagatg tggccgatac cattaaaggt caatgttcct ttttctcgga
    16081 tcactccgtg ttgcaaaata tgcccgatcg tatgaggggc tttattggtt actcgtgtat
    16141 cgattccttg gatttgtctg cccgagctga ttgccacgat tttgacttct gcgtgtgcac
    16201 cttctcctgc taaatctgag tcaaagtcag cgatcacatg accgtcattc atgacaccaa
    16261 ttgcccagtc gacagaagca tctcgtaaaa tgtgcccacg gcgattcata taagaagtaa
    16321 tattttctcc taattgatct acggctgaat atttgatttt tgaaccagct ttagcgatga
    16381 cttccacgat gatattacct gatgattttg ctacttgttc tttggttgtc tggaaacgtt
    16441 ccagataaga aaattcacta tgttcatctg ccacgatcaa cacatgcttg aagaagtgct
    16501 catcggatgc gccatcttga atgaataagg actcgagtgg ttcatcgatc acgacatttt
    16561 taggcacata caggaaaata ccgctgttca taaaagctac atggagtgca gtcaattggt
    16621 cttcgttcat gtttactgct tttttcatat aatattcttg gactagttct ggatattcga
    16681 tcattgcagt gaacaagtcg gtaaaaatga ctcctttttc tgctaattcc acaggcagtt
    16741 gttcaaaaat cgtccatgaa ccttgttgga caagcacagg attgtctttc attgcatcaa
    16801 atgccggaat cgtgcctgtt tcactgatgg tttgtgtttc tttcacatca aataacgacc
    16861 agcgatggaa tttcacccga tcgatatgcg gcaatggcag ttcatctgct ttcgctagtg
    16921 cgtcttgacg gaaggttctc atccaatcag gttcgccttt acttaaagaa aaatcattta
    16981 ccgcgtcaag ataatcgagc cagttttttt ctttcattct tgctccccct tacgcttctt
    17041 cttctttata gtcgattcct aattctttac tgataccagc gtagccttct gcttccagac
    17101 gtttagctag gtcagcattc cctgtcatga ctactcggcc ttccatcatg atgtgaacga
    17161 catcaggtgt gatatagttc aataatcgtt gatagtgggt gatgatcaat gcaccgaaat
    17221 tgtctccgcg catttcattt actcctttag caacgacttt caaagcatcg atatccagac
    17281 ctgaatcgat ttcgtctaaa attgcaaatg taggttctaa catcaacaat tgcaggatct
    17341 cattccgttt cttttctccg ccagaaaaac cttcgttcag ataacgttca gccatttctt
    17401 ctggcatatt tagtaaagcc attttttcat ctaacttttt caaaaatgcc ataacagaaa
    17461 ttttgtcgtt ttcatcacgt ttcgcattga tcgctgcacg catgaattcc gcattcgtga
    17521 tacctggtat ctcacttgga tattgcatag ctaaaaataa gccaagtcgc gcacgttcat
    17581 ctacttccaa gtctaaaaca tttttcccgt caaacaaaat ttcgccttct gttacttcat
    17641 agtttggatt gcccatgata gcggcagata aggtagattt tcctgtgccg ttaggtccca
    17701 tgatggcatg gatttctccg gttttcattg ttaggtttac gcctttaagg atttcttttt
    17761 cctcgatcga cacatgtaga ttttttattt ctaagacaga catgcattat acccccaaat
    17821 tctctttcgt tctcactcat tttagactaa ttgagaatga agcgcaatga ccttccttac
    17881 taaaatcaaa tatcttttat ttattattaa acaattttga tttctaaatt agaataggta
    17941 taaaaaagat gagaaataag ccattacaga cttatttctc atcttttttg ttccatacct
    18001 tattttgcat cagcctttac tggtttaaca gagccgcccc atttgtctgt gatccattct
    18061 tggacttctt tgcggtgaag cacttcaatc agttttttga ttttgtcact gtcttcatca
    18121 cctttacgag tcgcaataat attcacgtaa ggagagttgt ccttttcaag caatacggca
    18181 tcttctaatg gattcaaccc tgcgccgtaa gcgaagtttg cgttgattgc aactaggtca
    18241 ccttcatcat tttggtacgt agaagctaaa agtgatgggt cgatcgtatg gttgaacttc
    18301 aagttcttag ggttttcttc aatatcatcg aatgttgctg tttccagatc tactccatct
    18361 ttgactttga ctaagccagc atcttgtaga attgtgatga tgcgtcccca gtctgattca
    18421 gaatttgaag taatgattgt tgcaccatct tttaattcgg aaacatcttt gactttttta
    18481 gagtacaagc ccattggttc gatgtggaca gcaccggcat tgacaaagtc atatcctttt
    18541 tctttgattt ctttgttcaa ataaggaata tgctggaagt aattggcatc aatgtcgcct
    18601 tcttccaaag attggttcgg taatacatag tcatcgaatt tgacgatttc aagatcgatt
    18661 ccttcatctt tcaataatgg tttcacatgt tccaaaatct ctgcgtgcgg tgtcggtgaa
    18721 gcgccgactt tcaatgtcgt gtcgtctgct ttatttgctg aatctttgct gtcgctgttg
    18781 ttaccgcatg ctgctaatcc tacagtcagc aaaagcgctg ctgcaaatcc aaaaattttc
    18841 tttttcatat cctacatccc cctatatcct attatctttt gtctgttttc ttagttaaga
    18901 aatcaccgat tccttgaacg ataaatacga ttagtaaaat aattaaagtc gcaacgaatg
    18961 tcactgttag attaccacgc tggtagcctt cctgccaagc aagacctcca agtccacctg
    19021 cgccgatcgc gccggccatc gcagtaaatc cgatcatcga gatcgttgta actgttaatc
    19081 cagaaatcaa tgcgggaata ctttcaggta tcaagacctt ccagatgatt tcccagtaac
    19141 ttgctcccat tgcatctgct gcttccagta caccagggct gacttcacga aaagcaatct
    19201 ctactagacg ggcataaaat ggtgctgcgg ataaaacaag agctggaatg gctgcttgcg
    19261 cacctagcat agtgcctacg attgctttag taaatggaat gatcaaaacc atcaggatca
    19321 taaatggcgt tgaccggaaa atattactga tgatcgatac gattccataa atgattcgag
    19381 catacggttt gtttttgcgt ccaatagagt aaaggatcaa tcccaaaact aatccgatga
    19441 tcgtcactaa gaccattgag attaatgtca tatatagtgt atcaaggata gcctgattca
    19501 tcgtttccgg gttgatttgt ttaaaatcta gatatgtttc tgaaaagctt ttatccacga
    19561 tggatcacct caattcctac tccttgttct ttgatgaaca cttctgcttg atctaattct
    19621 ttggtttcac cagtcatcaa gacagtcaat gaaccaaagg aaccttcttt cacttgtttg
    19681 atctttccat acacaacgtt gacatcgatg ttatagcgtc gaatcgcttg ggaaatgact
    19741 ggctcgttgg catttctttc gctaaaggtc aaagttgcca aacggccgct tggattttct
    19801 ttgatgagtt cagctaatag ttctgtcgaa tcagcttctg gttcgatgtc ttgttgtaca
    19861 aagcgtttgg ttacttcttt tttcggttgt ctgaagacag tcaatacgtc tccttcttct
    19921 acgactttcc ccaactccat cacagcgact ttgttgcaga ttttgcggat cacattcatt
    19981 tcatgtgtga tcaagacgat ggtcaaattc aatttcttat tgatcgctaa caataagtct
    20041 aatacttctt ctgttgtttg cgggtccaat gcgctagtag cctcatcaca aagcaggatc
    20101 tcaggatcat ttgccaatgc tcgtgcgata ccgacgcgtt gtttctgtcc gccggaaagt
    20161 tgtgaaggat atgcttgtcc gcgtccttct aacccgacta aacgcaacag ttcttctgcc
    20221 cgttctttac gtttgctctt tggtacgcca gctaattcca gcggcagctg gatattttca
    20281 agtacagttc ttgaccataa caggttgaaa tgctggaaga tcataccgat tttctttcgg
    20341 aatgtacgta attctttgtt ttttaatttt gtaatatctt gatcgtttac gatcacttcg
    20401 ccgctcgtgg gaagctcaag tccattcagg agacggacta atgtactttt tcctgcgcca
    20461 gaatagccga cgattccata aatatcccct ttttcaacgt ttagtgaaac gtcattgacg
    20521 gcttggatgt ttccgttttt cccattgaat gtctttttga cattcgttag ttggattaat
    20581 gacatgccat tcacatcttt ctcttataat ttttcaaaca aaaaagcctt cgccctcttg
    20641 ccttaagcaa aaggacgaaa gcttgaactt ccgtgttacc accttgattt accgttactt
    20701 cgcaataaca gcctcttcca gtacttggac ttagcttctt tccgtatact gtcgcgctgt
    20761 aacgggcact cccgtagtaa gtttgcttac gctcgcttac tctgctctaa gaccatcttc
    20821 catctcattt tcgttaccca ttttcagcaa acagggctct cttcaaacgt ccgtgacatg
    20881 tactcttctt ttcaaagctt tctcgtttca tataggattg cttatgatta tagcaactgg
    20941 aagaatcagt gtcaactttt ttcaagtgat tttctgattt tataattcgt caaattcttt
    21001 tggatcaaca tctttgaaac ttaagatcca cgcattcatt tcatctgaat cattcaagat
    21061 attgatatct tcaccgattt tatcattaac agaagaaact gtacccgtta gcgggctggc
    21121 aaattcattg accgcttttt ccgcttcaac ttcgatcaga ggttcatctt tttttagcga
    21181 ttgtcctgct tttggcagat tggcaaacgt gatgtctcca agttcttctt gcgcttcatt
    21241 tgtcaaacca acacagtatt cttttccatt aaataagatc caaaggttgt cctttttctt
    21301 caaatgcttg cctgccattt tatatcagtt ctccttcata aattttttca ttgaatccaa
    21361 ttgcaacaag ctggtcctct cgtaagatca gtggtcgctt gatcagcata ccatctgtcg
    21421 ataagacttg gctcgctttt gaagccgtaa actcgcctac ttgatctttc agtccaagtt
    21481 cccgatattt catcccgctt gtgttgaaca ggcgacggat tggtacttga ctcgtttcta
    21541 gccaagtctc aatcgttgag gccttcggta cttctttgat catatcaacg gtttgataac
    21601 taacgccatg ctggtctaac cacgctttgg cttttttgca tgttgaacat ttcgggtacc
    21661 agtaaaaggt gtacaatttt cttacctcct tgaagatctt gcaggtgctt cattttggta
    21721 tctcatcgat aatatcaacc cgataccgat catgttccct aaaatcgagg atcctccctg
    21781 actaatgaaa ggcaatggga tccctgttaa aggaagtaat ccgatatttg ctccaatatt
    21841 ctcaaagaca tggaacagca gcatcatgat caaaccgctt gcgatatagg cgtaaaattc
    21901 attattcgta tcgaaacaca cccgaatcat acgatagatt aaaataaaat acaagaaaat
    21961 gacgaatgca ccaccgataa agccgaagtt ttcaccgata acggtaaaaa tcatatcgga
    22021 ctcacgaaca ggcacataga tattgcttac gttgaatcct ttaccaaaca agccaccggt
    22081 accaattgcc aaaagtcctt gggccggctg ataactcatt cctgacgtat catggaaagg
    22141 gtccagccat gaatcaatac gtgcaaactg ataagatttg aatccaactt tataaagcaa
    22201 gtctcttccg ccttcagtcg taaccaagaa gatcgttcct gctccaacga ctactgccaa
    22261 gattactaca ggtgcgatga tgcgccaaga gattccagac atcaggaaaa tcccgccaaa
    22321 gatcgcaaga aagacaagca tcgttccaaa gtcatcttgg gccatgatca atgctaaaac
    22381 cggtattgtc acgatcaaga gcttcccaat cagccaaaag tccgatttca tcgtccgttc
    22441 ttttacttgc gtattatgtt tagttacgat cagtgccatc atcaagatgt acgcaatctt
    22501 catcaactct gctggctgga aagtaaacgc accgaatcga aaccagtttt tagaccctgt
    22561 tgaagcagct aaattctgat catagaattt gagcaagagt cccattacca ctaaccccaa
    22621 accataaagg taaggagtca gtttccagat ccatttggaa ttcaaatgca tgatgacaac
    22681 gattgctgcc gctccaacta aataccagac aacttgcatc cctacgcctt tcaagactgc
    22741 tggattgttc ggatcatggc taagtgctac atataatgag agcagaccaa tcaaacataa
    22801 cagaaagact ggtaaaatca cgccataatc aatacgatta tcgttcgaag cttttaattt
    22861 tttttccatg tttcatccct tttcttaaaa accatactgc tgattattat agcggtccag
    22921 ttagataatg gaaagtcttt ccatagacat ttgggaaaaa ttaactttcc gtttgtgcag
    22981 ttgcttgagc aaatgcagta caaaagccta ctgcttatct ttagtgtatc atagaacttt
    23041 ttttgataca tgcaattatc taccagctga gattctcctc gctgtgagaa gtttctgtgc
    23101 tttcattgta tcgaaggatc tcttcggcaa tctctagcag ctgatcagaa accagattgg
    23161 ttcgttggat gaaataaaaa tatcgatgga aattcaacgg agtcattggc acattttctg
    23221 gaacagagcg cttagacaaa atcgagcgac cgattccttc cttcagcaac gcaacaatta
    23281 catcgttact tttcacgatc atcttcttat cttggatatt catctcttca aaataacgct
    23341 gcgtgtaatg aaaaacacca gagtttgctt ctcttacaag ccacaatttg ctttttgggt
    23401 ccccagcgat gactagctgg tcttccatca aagagtatct ttgtacgccg gaagtctcta
    23461 atggcttttc gataaaacca aaatcggatt catgtttcgc tacttgttct aatgattcat
    23521 gagaattcgt gatttcgatc tcaaaatgca ctttaggaaa ccttttgatc agatgtttca
    23581 tcaactgagg caaaagatag acagcaaagg tgtgggaagc aacgatccga catacttctt
    23641 cttcattttg ttcattgagc aatttttgct gagtttcttc ccattcttct aacaaaacca
    23701 gtgtccgttc atataataag tcggcttgct tagttggaag catctctttt cttccgttac
    23761 gttgaaaaag atcgatattc aattcccgct ccaactgttt gatttgattc gagactgctg
    23821 gttgagataa aaacagtact tctgctgctc tggaaaaatt tcttgtttca tatactgctc
    23881 gaaaagtcgt taataactta aacatggcac cacttccata actttttatg ataattacta
    23941 taaaaactat ttgatttaaa tatacaaaaa accatctata atagaaatgt acaacaaaac
    24001 agttgctgat ccaactagta acttagatga agacaatcag ctgtctgctt gctcgtaatc
    24061 atacattcct attcatttta caatgtattc gttcatcttg gtactaaaat ctattaaata
    24121 aaaaggagaa tattatatat gaagaacttt ttgaaaatta ttccgatccc gatctgcggg
    24181 ttgatccttg ggttagcttc ccttggaaat ctgtttaaag actatcacca tgttgcttta
    24241 ggcaatctaa ctggcggtat ctctatgatt cttatgatat tcatttttgt taaattgttt
    24301 atcttattcg aacacacgaa acaaacgttg catgatccaa tcatcgcctc cgtttcacca
    24361 acttttacga tgtcactcat ggttatttgt acttattttg tttcatatga aagtattgca
    24421 ccaattgtca aattcatttg gctgtgcgct gttatcttcc aagtgatcct tgtcttttat
    24481 ttcaattatc accatgttat caaagctgat ctttcaattg aagcaattta tccaagctgg
    24541 tttatcattt acgttggatt tggggtcatc actgtaactg ccgggaactt cttcccactt
    24601 attggaaaaa ttttcttttg gatttcattg atctgctatg cgttcttatt gccgatcatc
    24661 atcaaacgtg tctttttcgt aagaaatatg gctcatccga cacgtccgct gattacgatc
    24721 gttgcagcac ctggttctct ttgcctaaca ggttatctta aaaactttgc tgaacctaat
    24781 ttttatcttg tcgtagcatt gttcgcttta tctcaagtat tgtatttcgt tgtgctgtct
    24841 atgcttccac gtttgttcaa gcttcgtttt tatccaagct atgcagcctt tacattcccg
    24901 cttgttatct ctgcaacagc tatatttact actgttcatt acttcaacag tttaggtatc
    24961 tatagtagcg tattggattt cttaaaagtt gcggaatgtc tgtttgcaag tgtagttgtc
    25021 ttctacgttc ttgctcatta cgtacgtttc ttaattaaag aacatagcaa acatggaaaa
    25081 tttgtttcta aaaaaatcgc ttaataatcg attcaatagg ttgtgaaaaa gctgttctgc
    25141 atcaagtaat aagaacggcc aaacaaaaac agctgttcat gtttttgtac tggttgggct
    25201 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggtcgtaaa agaagtggtc
    25261 agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg tgaaatttcg
    25321 gcttatttcc gtagaagcta cttctggaac accgtttatt cgaaacaaga ggtcgtacaa
    25381 gacttttgtc acgacctctt gttcttttgt caaaatactg gtttagtgct actatgttcg
    25441 agtatgcttt tagtatttat tgacgaaagg aaccgatcaa atgaaatcat tgtcacatcc
    25501 aataaaatct gtaaaggatt cagcggtccg cgaaatctta cccggtttac tgctttcttt
    25561 tgccatcgct gccttcagca ggcttcttgc tatttatctg ccacagttag gtggagcgac
    25621 tattgctatt ttattgggta ttttattagg aaatactttt tttaaacagc ctgttctagc
    25681 aatcggcaca aaatttgctg aaagcaaact attagaattc tctgtttttt tactaggtgc
    25741 aactgtcaca gctcaaacga ttgctcaaat tggtgtaaaa ggaattgttt ttgtgttact
    25801 gcaaatgtca gtcactatca tcggtgctta cttgattggt aaaaaactgc aattcagcga
    25861 tacttcttct ttgatgatgg ctggaggaaa cgctgtttgt ggttcttctg ctattgcttc
    25921 aatcgcccct tccatccatg cgaaagagga agaaaaagga cagatcatca cactagtcaa
    25981 tctactgggt actgtgatga tgttgactct cccttttctt ggattacagt tgtttggtgg
    26041 aaatctcatt gaaaaaagtg cacttttggg tggtacactc caatctgttg gtcaagtagt
    26101 cgctggggca agcttacttg acaattctgt tgtcgaattc gccatgctat ttaagattct
    26161 acgtattatg atgctcgttg ttgttgtcta tctgtttgaa aaattcatgc acaagaaaca
    26221 accagctctg tttgaaacgc caactgatac gcagaaaaaa cgaaaattac cgcttccttg
    26281 gtatgtatta gggtttgtga tcttttgttt ggtcaacagc ttgttttctt tacctgtagt
    26341 tattgaccaa tcggcacatt ttatcagcag ctggtttgag atcactgctt tagcggcaat
    26401 tggtctgcgt ttagactttg caaaattcat gaaagaaggt acacgcttct tatcttatgg
    26461 attagctgtc gggcttctcc aaacagtgat ggctttacta tttatttggc tgatcaaagt
    26521 ttagaatgaa catgaaaaaa cagttgtgat tctcgcatgg aacaatgtcc gaatcacaac
    26581 tgtttttaat tttgcagata tccttctaac aacaacaatt ggcgtttgat ttcaacccca
    26641 tctattgcgt ttcccatata tcctgtcagt tttccatttt gcccgatcac tcgatgacaa
    26701 ggaataacga ttggcaatgg attggttcga tttgcctgcc caatcgctcg cacagctttc
    26761 ggactatcaa cagcaacggc tacttcttta tacgttcgga tttctccata aggaatggtc
    26821 gacaaagctt tccagacatt ttgctgaaaa ggcgtacctc tctttataga taaaggaaca
    26881 tcaaattctt ttctttttcc cgcaaaatat tcttcaaact gttcttgtgt gcgcgttaaa
    26941 attcgctgat tcgctgattc gctttctgaa attggctgaa aagatacagc agtcaatccg
    27001 gattcatctg catctaacca taaaaagccg ataggtgtgt gtattttcat tttgtttgct
    27061 cctagtcaat agactggaac ttcctccttt ttctctatcc aaaactactt gccaactaat
    27121 aaaaattgtt cttaaaagtt tgataaaccg aggaaacatg gtaaactaaa tacgttagtt
    27181 tacaaaaaga atacctgttc ttttacgtaa aatcaaattt taaaggtgtt atttaaggtt
    27241 tattaaattt tgaatgtaaa ccatatataa acatacgatt gaaaggatag aaacgatgaa
    27301 agtatcaatt gttgtcccat gttttaatga agaaaaaact gttcctcttt tttttgatga
    27361 ggttgaaaaa ttcaaaggtg cccatgagtt cgaatatttg tttattgacg acggatcaaa
    27421 agacggtacg ttagcagcaa tcaagaagct agcctcagct tatccttatg tccgctttgt
    27481 ctcattttcc agaaattttg ggaaagaagc ggctctgtac gctggattgc aagcttctac
    27541 aggagattta gtgaccgtga tggatgttga tctgcaagat ccgcccgaac tgcttcctga
    27601 gatgatcaac cggatcacta cttctgatat cgattgtgtc ggcacccggc gttcgactcg
    27661 tgaaggcgag cctgcgatac gcagtttctt tgcgaaaaaa ttctaccagt tgatcaataa
    27721 aatcagcgat acagaaatgg tcgatggcgc tcgtgatttc cgcttgatga ctcgtcaaat
    27781 ggtggatgcc gtcctagaat taacagaata caaccgattt tcaaaaggat tatttagctg
    27841 ggtcggattt aaaacagaat atatttctta tgaaaaccgc gaacgtgtgg ctggcgaaac
    27901 atcttggtcc ttctggaaat tattcaacta ctcgattgat ggtatcgtga acttctcgga
    27961 tgcaccgtta acgattgctt catttatcgg cgctttgtct tgtgtcggtt ctggtattgc
    28021 aattttgttc attattttgc gggcattgtt gtttggtgat cctacatcag gatggccttc
    28081 catggtctcg atcttcctgt tcattggtgg aatccaattg ttatgtcttg gaatcatcgg
    28141 gaaatatatt gggaaaatct tcttagaaac gaaacagcgt cctgtttata tcataagaga
    28201 aacagaaaaa gtgaagccaa atgaattgaa gcctcaccaa taatatacgt caaatgaagt
    28261 aaacattttt ttaaatgaaa aaggactgtg acaaaagtct tgtcacagtc cttttttccg
    28321 aataaatggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
    28381 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct atcacaacct
    28441 ctttcatatc ttattacttc ttcattgctg cttttagtgc tgctgctaat gggctttctt
    28501 ctggttcttc tgcttgtgca tattttttca gcaagcgtcg ttcttcatgt ttggtcattt
    28561 tcttcttctg ctctttttta tcaagcaatt tttctgtcgt tccatcgtat ttacaacgga
    28621 aataagcacc gtttttacct tcgatgattt ccattttccg gtgacattgc gggcatcgat
    28681 gattagagac ttttggatct tttcttctgc ggtagttgca atctgaattg gtgcagacat
    28741 agattttgcc gtcacgtgtg tttttctcgc gcagattggc cccacattct gggcatttct
    28801 tttgggtaat cgagaaatct tgatattctt gtttgcttga tttgatctca ttcaccaagc
    28861 gttttgtttc tttttcgatt ccattcaaga aggttcctgc ttgtttcttt cctgcagcaa
    28921 tagcttcaag gtctttttcc cagctttctg tcaaatctgg tgttactaaa gagggattta
    28981 ccaatactaa taattgctta cctttaggag atacttgcag tccttgaggg gtacgttcca
    29041 tcagttcaga tttgattagt ttctcgataa tctctgcacg cgtagctggg gttcccagac
    29101 tgtatttttc catttgacca agcaagcttg cttctgacaa tggtttaggc ggtgcagata
    29161 gttctttttt gatggcgaaa tcgggattga ttctcattcc tttttcccat ttgactgttt
    29221 ccgtctcagt ttcagtttcg cctttccatc ctgcttggac gacacgattt tgccgaaaaa
    29281 tgaatcgttc tttaccaaat tcaaccgtaa cttttgtttg gctgacttta tggggttgag
    29341 caaacagtcc taaaaaacga gagacaatca tttgatagat ttttcgctct tcattactta
    29401 atttttcata acgtgctctg ttttcagttg gcaaaagtgc atggtgatcg gtcactttac
    29461 tgtcttggaa tacttttgtc tgttggacga tggctccgtt tttcaaatac tgcttgacct
    29521 caggagcaaa gtcgctgact gcttgcaatc gttctttcat cgtacttttc atgtcatttg
    29581 tcagatattt gctgtctgta cgcggataag taacgatctt atgggtctca tacaaacttt
    29641 gtacaagact taatgttttc tttgcagaat agccatagcg ttgattggct tctctttgga
    29701 tctctgttaa atcataaggc aatggcgcag attccgtttt taatttttct tggacttctg
    29761 tgacagttcc tttgcttgta ctgatcaatt gaacaagttt ttgtgcttct tcttgtgttt
    29821 ttaaagcaaa ttgatttttt tgaagcattt tggcttgttc ttttccagct tgcaacgtga
    29881 tcgtgaaata cgtttgtgga cggaactgct cgattttctt ttcttggtct ctcactaatg
    29941 ctagtgtcgg tgtctgcaca cggcctgctg ataaattatc cgcgtacttc acagttaagg
    30001 ctctggtcac attcaatcct accagccagt ccgcttttgc ccgggcaaga gcagattcat
    30061 acaatgcatc atattctttc gcaggtctta gttgcttgaa tccagttttg attgctttat
    30121 ctgtttgaga agagatccat aaccgcttca ctggtttatt gaaatgcaca tactctaaga
    30181 tccagcgagc aacaagttcc ccttctcttc cggcatctgt cgcaatgact gcttctgaga
    30241 catcttttcg ctgagccagc tgcttgattg cttttaattg atgtcctgtt ttaggaagtg
    30301 gtttgattcc tagtttttta gggatcattg gcaacgtgtc catttgccag ctctgccatt
    30361 ccttattcag atcttccggc attttcaatc ctaataaatg accaagggcc caagtaacga
    30421 tcaccttggg tccttcataa taatttttgt gcttttgatt agcaccaagc accttgctta
    30481 ggtctttcgc aacgcttggt ttttccgcta gaattagttg tttcataaaa ttcctttctg
    30541 ctttctactg tgatgggctg atgatttttc aacaacgcaa gtcccttgta ttgctgtaaa
    30601 tcatcgtcac actcttcaca ccagcgctca tctccgtcat tccaaaaggc gctgagaaag
    30661 ttactttttg cattgatata ttcataattt tgatggattt cgttccattg tttttcgtac
    30721 gcttgtcgtg cttcttcgaa agactcgaat gtttcttctt cttcaatatc ttcttcccag
    30781 tcttcaaaaa accaccaagg ctcattgtcc ccgaacatgg taattacttg atacatactc
    30841 gtgctccttc tttcagcttt tctagactca tcttagtcac ttttcaagaa agttgcaaga
    30901 attttctctt ataaaagggt catttaatga acatcttaaa attgttacaa atgaatctga
    30961 ttgtttaagg caaagtagcg ataaagttat tgatcatcca aaaatagctg tcttctggat
    31021 cgattgttgg ttcttttgta aagtatccta aaaaacgtaa ggaaagataa ccgtggatca
    31081 tactacgaag ttccctgctt cgatgaactt tttcttcttt cgttaattga aaagcctcaa
    31141 atatctgcaa aatgatctga ttcgtttcat ggattccatc taataaaaca gcttcattgg
    31201 tatgcggtac actgatcagt aactcataca ctgcttgatt tgaaaaagca aaggatcgat
    31261 aagtttctgc atagatctgc agtgcttcct gaccactttt tcccacaaga gcccgtcgca
    31321 gctggtcatt aagttcctgc agaagtttcg ctgttagcgc tgttttgaca tcacgaatat
    31381 ttttgaaatg gttatacagc gagggagcct tgatatccaa gcgtttggct aaatgctgaa
    31441 aggttatttg ctgaacatcc atctcttctg ctagttctcg aaagcagtcg atgattttct
    31501 cttgagacag atttcttttt ttcatttcca tgtttcctct cataaaataa atccttcact
    31561 ctttcagttt accacgaaaa agaaagagaa aaaagagact ttcattgata aactacatat
    31621 tgtagtttat aataaagagc tgtagatgaa aattttcaga aagaaggagt ctaattggct
    31681 gcaagaataa aggaaaaatt atttaggaaa agagaaactt gggaacaaaa cttgtttgtc
    31741 ttatggtttg gaacatttat ggctggtatc ggatttagtt tagtcatgcc ttttatgtca
    31801 ctttacatag atacgttagg taattatagt acgtcacaat tgaacttttg gagcggattg
    31861 actttttcat ctactttctt ggtgactacg cttatctctc cttggtgggg aaaattagcc
    31921 gaccaaaaag gaagaaaatt gatgctgttg cgtgcttcat taggaatggc aatcgtcatt
    31981 tctttgatgg gttgcgtgac tagtgtgtat caattagtcg cattgcgtct gcttcaaggg
    32041 attttttctg gttatatcag taatgccact gcccttgtcg caactgggac gccaaaagaa
    32101 aaaagtggtc aagttcttgg aaccttagca actggttctg ttacaggaac attacttgga
    32161 ccgcttcttg gcgggatctc tgcttcagcg tttggctatc gccctacctt tttcattaca
    32221 gggatcatct tatttttcgt cttcttgttg agtctgtttt tcgtccatga aaaatttgtc
    32281 ccagtgaaaa aagaaaacat ggtttctgcc aaacagattt tcaaagactt gaaatatcca
    32341 cacgtcgtca tcggtatgtt cgtcacaacg atgatcatcc aagcatccaa caattccatt
    32401 agtccaatca tcagtctgta cattcgccaa ttgctgcatg gacatgggaa tgttacgttg
    32461 atcagcggaa tcattgcttc gatacctggg attgccactt tgatagccgc tccaagattt
    32521 ggtcgcttag gtgacaagat tgggagtgaa agaatacttg cgattggttt gggttttgcg
    32581 attctggttt atatcccaat ggcattcgtc acgaatgttt ggcaattagc tgccttacgt
    32641 tttttgattg gtatctctga tgcttgtctg ctccctgctg tccaagcatt gatcacaaaa
    32701 tattcgcctc atcaagctgc tggacggatc ttcagttata accaatcttt ccaagcaact
    32761 ggtaacgtag tagggcctat gatcggctcc agtgtttcca gtgcatttgg ttatcgcggc
    32821 gtctttcttt cgacttctct tcttgttttg gtcaacttct tgcttgtacg acacaataca
    32881 caagagatcc atcagcaaga aactgcagaa gaaaaagctt caggagtctc catagcttca
    32941 gggcactctc cttctcatag acaataagcg taaatgaaaa acactgcttg aacagttgtc
    33001 acagactgtc cttgcagtgt ttttgtttag gcctgttttt tcaagaactc agataagagg
    33061 aataatccct tttccaaggt ttctttttta caacagtaac ctagtcgggc atgcttcggt
    33121 aaatcaaagg cacttcctgg tactaatagt actcctgttt cttctaataa attgatacaa
    33181 aatgtctggt catcttcttc gatggctaat ttaatgaaag aagtagagac gtaattggga
    33241 gcgacaagtt caactttcgg ttcattggct acccattgtt ttaaaatcac aaggttctct
    33301 gtaatgatct ttttgtttcg cgccaatatt ttttctttat tcttcaatgc atgtacagca
    33361 agatcatctg acaggactcc gccgcaaatc atcgtgtagt ctcgatactt tcgaaaaact
    33421 tctgccaact caggtccagt agctgtccag ccaatacgaa tacctggaat ggagtaggtt
    33481 ttggataggg aattggttgc tatacctttt tcatagacat ctacgatcga caagaattca
    33541 cctttatccg ttaaaggcgc atatacttca tctatcaata catatgcatc tactgtacga
    33601 gcaatttctg cgattttttc taaagttttc cgatctagca atgtgcctgt aggattgttt
    33661 gcactattca aacagatgat ttttgtttca ggtttgacta aggcttctag ctgttctaaa
    33721 tcaaactgcc aatcttcttc ttccttcaaa tgaacaaagt caacagcggc acctaaagat
    33781 ttggggatgt catataattg ctgataagta ggaagcatcg aaacaacatg gtctcctgct
    33841 tccactaacg cgtatagagc taaaagatta gcaccagtcg ctccattcgt ttgtaagatg
    33901 ttttccggat cgacggtttg ataaagagaa gcgacttctt gtttgaaaac atctgatcct
    33961 tcgatccagc catagtccaa tggttcgttt ttgcgttttt cgaaaaattt acttacgcta
    34021 gtcccatcta agccaacgag ttcgtctaat gtcaatgctt cgatcgagct ttgtgcaata
    34081 tcatataccg ctttcttttc ccatttattt agccattctt ctacaccgaa atttgcgatt
    34141 ttcatgtttc tttccccctt atatcttgat ccagcctaca gcaagcaacc cgattgaaag
    34201 cagtgcggcc acacaaatca gcccaatcat tactttttct tttcccgtaa tttgatgttt
    34261 ggtttcacga caagcgatcc agtagaagca aaaaccagga agataggcaa tactacataa
    34321 aaggacataa gaaaatcctg ctaaaatgat gcccatcaat tcaaaaacta cagcaatccc
    34381 accgatcact acttgttttt tgattccttc ttttaaagaa aactggactt gatagatccc
    34441 gacaagtaga taacaaatca aaattgccgc tgtacataaa gaataggcaa attgataggc
    34501 ataatcagta actaagaaag tcaataagaa tagattagtc aagattgcag taacaactaa
    34561 cgaataagtt ggggcatttt ttttattcgt ttttccccaa cctttcggca acagaccgtc
    34621 tcgagccatc aacatcgttg tttctgctgg gagcatcgtc caagacaacc aagaaccaat
    34681 gatcgaaata atcaagccga tattgatcag cgctgctccc caatttccca cgattccttt
    34741 taaaatattt gccatagcag gttgtgaaat agttgctaac tcttcttgag ataagacgcc
    34801 gtatggcaag atcgaagcaa ggatatagat aaacaataaa caaagcagac caatgatcga
    34861 tgcttgctgg gcttctgaac gtttcttcgc gcggttggct aaaacacttg cgccttcgat
    34921 ccctacaaag acccacatca taaccatcaa acaaccttta atctgcaacc agatcgttcc
    34981 ttgctgccct gttccattca ctagattatc agatacgtta ccccaaaaat cagcagtgaa
    35041 tatattcact ttaaatgcga taaatacaaa tattaaaaat aggaataatg gcactaattt
    35101 acagatcgtc acgattgtat tgatgacgct cgctccttca actccattgt ttactaataa
    35161 agtcagaccc caaataaaaa tacttgcaaa taaaatagaa ggaacatttt gtccaccttt
    35221 gaatatcggg aaaaattctc ccatggcgct catcagcatc gttgcaaaag caacatttcc
    35281 cagccacgca gacaaccagt aaccccaacc actgatgaag ccacctaatt taccaaatga
    35341 agcttccgcg taaccgaaaa taccaccatc tagatctgga cgcttttctc ctaaatgatt
    35401 cagcgataat accaaagcta aaataccaat cccaacgatt atccatgaaa ggatcgctgg
    35461 ccctggagct gcagaactag ccagatcact tgtgatccca aacacaccgc ttccgattga
    35521 cgaactgaca accagagcaa ccagtcccca tttgctaata cccgtcgatt tcttttccat
    35581 ttccatcacc ctcacttata aaattgaaca aatcacttgc aaaagattcc cttctatttt
    35641 gcaactgctc ttgcttaaca aaaagcaact ttattcatat tattggatat tatgaacttt
    35701 ttacacaagt atttcaagta taaaatgaat aaaaattaat tttattgcaa aaaaacagct
    35761 atgctatcca aaagaaaagg tgtttctttt gttagccata gctgttttga ttcatttaat
    35821 atcgcttaaa cgtattgtaa aacttttgtt tcttgatatg cgtggtcgat caaaccacca
    35881 cccagacatt ccattccatc ataaaataca actgcttgtc caggagtgat tgcacgtaca
    35941 ggttcgtcga atacgacttc tgcacgatta tcatccaata agcgaactgt cacaggaacg
    36001 tcttgctgac gataacggaa tttcgctgta cacttgaatt cttttggcat tggttcattc
    36061 gtcgtaaagt ggatttcact tgcatccaag cttgtggcat acaatgcagg atgatggaac
    36121 ccttgcccca catataatgt gtttgtcgct agatcttttc ctacaacaaa ccagggttct
    36181 tgcgaatctc ctccgccacc gatccctagt ccttgacgtt gaccaatcgt ataatacatc
    36241 aagccagcat gttgtccttt gacttcacca tcaagagtga ccatatttcc tttttttgct
    36301 ggcagatagt tgctcaaaaa ttgtttgaag ttcttttcac caataaagca gattcccgtt
    36361 gaatcttttt tcttagcagt cgcaaggcct gcgcgttcag caattgcccg tacttctgat
    36421 ttttccatgc cgcctagagg gaacatcgtt tttgaaagct gttcttgtga taattggcta
    36481 aggaagtaag tttgatcttt attattgtct actccacgaa gcatgtggac cacgccgttt
    36541 tcatcgcgag tgacttgcgc ataatgtcca gtagctacat aatcggcgcc aagatccatt
    36601 gcataatcca agaatgcttt gaatttgatt tctttgttgc acatcacgtc tgggttaggt
    36661 gtacgtcctg cgcggtattc ggctaggaaa tattcaaata cacgatccca atattctttt
    36721 tcaaaattca cagagtaata aggaatcccg atttgagcag ctactttcgc tacatcctta
    36781 taatcttccg tggccgtgca tacaccgttt tcgtccgtgt catcccaatt tttcatgaat
    36841 attccgatca cgtcgtatcc ttgttccttt aacaaaagtg ctgttacaga tgagtcgaca
    36901 ccgccgctca tccctacgac aacgcgtgtt ttgctgttgt ctgtcatgat atcaccatca
    36961 tttcaattag attctgaaac atctacgagt tgaaggtcgc aacttttcag tattttacat
    37021 tatacgtcat atttctgaaa atttcatccc ttaattatca agtcgtaaaa atactgttct
    37081 actggaccta ttcagtacac aaacatgata gtcaaccaaa aaatcactca tcttttgagc
    37141 attcgatata aaaaattggc atactcataa aaagggccag cctttcggca ataaggaata
    37201 attttataaa ttgtgaaaaa ctatttttca aaattgcttc ttatcgctta aagctgcacg
    37261 tctcatttac aaccttttct ttttagatgt tatgagttgc tcagataagt gattttgttt
    37321 actctaagaa aaaagggagg caaaagatac atgaattact ttatcagtga tatgcacttt
    37381 ttccatgaac gcttattagg aaaaaacgac tttgctccgc ggctctttgc aaatgtggat
    37441 gaaatgaacc aacagttgat cactagctgg aatgcagtaa taaaagaaac cgaccatgtt
    37501 tatcatctag gcgaccttgc tatgcatccg caatatgaaa aagcaaatgc agaaattttg
    37561 gaactggtca ctcaactgca aggaacaatc cacttgatca aagggaacca tgacagtcgc
    37621 gcttttttcc gttatttaga aaaaaatgat cctaggctta ctgacggaag tcccaaattt
    37681 tatttttatg atgtaggtgc catcgttaaa ttcgaccatc aacaatatta cttgactcat
    37741 tatccgatgt tgctcgggcc taacgacact atccgcaatc ttcatggaca tatccatcat
    37801 tacagcgtac ctatcgagaa cgatgtcaat gtaggcgtag acgcaccgga aagagaactc
    37861 ttaaaggaaa aactaccatt tggcactccg ttatctgagc aacaaatcaa agaaatctat
    37921 gaagcaaaga ggaagtacga taggtcgtga aaaaactgcc ctatatcaag tattaaacaa
    37981 ataaagataa ttactcatat ttttcatttg agtaggctta ttgccacagg aacattagga
    38041 tcagaaatgg agttgtaaca agacttctgt cacaactcca ttccatacta ttcgtctttt
    38101 tgctcatggt gttcttttac cagttttttc tgtaattctt tatcataatt tcgggcagct
    38161 gacgttaaaa ataaaacgat tccgcataat aaaatcccga ttccggaaaa caagaacatt
    38221 ttttctaccc ctaaacgatc agctaatggt cctgcaaata ataatccaat tggacctgcc
    38281 aggttcatca aagagttgaa gttccctaag acacgaccta aaacattcga ttcataactc
    38341 tgctggatca ttgccatcag cagtgtattg aaatatggcg ttgcacaacc tgctcctgca
    38401 ttcaagataa gaaagtacaa gaacccttga ctatcgtttg gaaccaatcc gcttgcaccg
    38461 atcgtcaggc ccataatgat gtaggctata ataataggtt tcattcggtc tttccatgtt
    38521 cctataaaac tgatcagtgc accgcctaag agcattccga ctgcgtagac tacttccact
    38581 gctcccgcct gtccaacgga accaccaaag taatctagcg tcatcaatgg atacagactg
    38641 attgctggca tgaacaacag catcacaaat gcaccattga tcgttatgta ccataaccct
    38701 ttattatcca tcaattgctg cagcccaaat tttgcatctt taaaaatagt aagcggaact
    38761 tccaatattt caggagaaac ttttgggatc ttcacaaaaa tcaataagcc gacaccaaaa
    38821 actgccccca atacatctaa taaaataagg taattgacag gaacgactga aaacaaagcc
    38881 gcacctagag ctggcgcaat gatgaaattc gctgattgga ccattcctaa ctgtccattc
    38941 gtccttgtta tgtgagaaga tggaacgatt gtcggcataa tcgactggat cgttggcatt
    39001 tggaatgttt gtgcgaccga acgcataaac aaagacacaa aaactagcca aatcgggaag
    39061 gattctgaaa tcgttccgac tactgctaaa ataagcgcga aaatcgctac aataatatcc
    39121 gtgacaatca aaagagcttt tttatcccag cgatccacta gcggtcctac aaatgggctt
    39181 agtaaaacca tgggaatcat tcctagtaaa gtagcaaaac tcaaaattgt cgccgaaccg
    39241 gtttctcttg tcaaatacca gatgatcgca tattggacga ccatactagt gattcctgac
    39301 aaaaactgtc ccgataaaaa gaacaaataa tttctcttcc aattcgttaa aacgaccgac
    39361 tgttcatttt ccataaacat ccctcttttc tttttcattt tgtatagcgt ttgcacaaca
    39421 atcattatac gggattcttt tttcaaaaaa tacctttttc cctaaataac aatcgtttca
    39481 atttaaattt ctttacttag aaacaataaa tcagcaaaat attaaaaata acaacatgtg
    39541 ttaaatttaa aaataaaaaa gacaactaat ttgcgtcgtc cttacaaagt tagtcatcta
    39601 acaataggcc gtttcaaaac tagttgtctt tttatatcca cagatcattc gactttttcg
    39661 aataacccac ggctaatcat cccatccatc agaaaataaa atttatcttg gctcattttc
    39721 tgttttggat ctttgaaaat attgactgct ttcaatccat ttgctgtagt gatcgacatt
    39781 ttcaatgttt gaatatcttt tgatacagtg atttttaatt tgaatccttc tttgctttgc
    39841 ggcgtggcat ctaatggtcg gatcaattga taattgcctc cgtttgtttc tgtaaatcca
    39901 ttatcgcgca atgtatatct ttttgcatca ggatgcaaac gatagctaat tggtgcgccc
    39961 agtaccgttg ctgttgtttc aaatgccatg attttcacct cgataaaatt agtttctctt
    40021 tattataaaa gtttatcctg atttttacta ggtttttcag ttaagttccc taaaaaatga
    40081 aggcgttctc tcagacatct gttcatataa gaatgacttc actagttatt ttttgacagc
    40141 acgaactaat tgatcaagga aatactggat gtcgtcttcg gtatttccca aaccaaagct
    40201 gatacggatg gattctttta gtatcggtgt atcttctcca tacattgctt ctaatacgtg
    40261 tgaaggctcg atatttcctg ctgtacaagc tgaaccagta gaaatagcga ccccattaag
    40321 atcaagcttc atcagtagca aatcatttgc ttcgccaatt aagcgaagat tcaacacatg
    40381 agggagttta ttcgttaaat caccatttac ttgataaggg atttcgtttg cttctaatgt
    40441 atcaagaatc aattgctgga atcctttata tttgttgtta cgtgcttcac gttcttcagg
    40501 tgacagcagt tcaactgctt ttgccatacc ccagatccct gctaaatttt ctgtacctgc
    40561 acgccgcttt tcttcctgct ccccgcctaa aagcaatgca gggatattta ctccgtctcg
    40621 tttatataag aaaccgacgc cttttggccc attgatttta tgagcagaga tacttaataa
    40681 atcgattcct aattcttctg gatggatcgt ttggtttccg tacgcttgta ccgcatctgt
    40741 atggaaataa gctggatgat ccgctaaaag ttcaccgatt tctttgattg gcatcaaatt
    40801 tccgatttca ttgttcccgt acatcacaga gaccaagatc gtatcttctc gtaacgcttt
    40861 tttgacctct tcgatcgaca gattcccttt ttcatctaca ggcagatatg taacgtcaaa
    40921 cccctttgtc tctaaatatt tcatggtatt aagaacagca ggatgttcga tagctgtagt
    40981 gattagatgt ctgccttctt tttgacgcga gaacgctgtt tctaagatag ctgtattatc
    41041 cccttctgtt cccccactgt taaagatgat ctcgtgaggt tttgcttgca gactgtctgc
    41101 aatgatttgt cttgctgttt ccaatttttc atgcgcgtgg cgaccaaatc catgtatgct
    41161 tgaaggattt ccaaacgtgt cttgcatgat tgccgtcatc gcttcaatta cttgtggatg
    41221 catgggggta gttgccgcat gatccagata aatagttttc acttcattca tcccttccaa
    41281 attattgcta ggcacattct agcataaaat agcgaacaaa ccaaagaaga gctcattttt
    41341 gtctagagga cgtttcatgg gcatagaaaa aaggagctgt gacaaaagtc ttgtcgcagc
    41401 tccttgaagc tgctcacttc tgtcacaact tctttccctc ttttttttgt ttattcgaat
    41461 tctttttctt ccagacggaa taacggactc atcggtgtat tttcatggat gcgtttgacc
    41521 gcttcaccca ttaattctga acatgtaacg attgttaagt tttctggatg acgattttct
    41581 tctgtgaaaa cagaatcagt gatacaaata tctttgattg gtgcattgtc taaaatactt
    41641 ttcgcatgat cggacaataa tccatgtgac gcacaggcat atacttcttt tgcgccgttt
    41701 tcttttaata catttgctgc tcgtgcaagc gttgctcctg tgttcaagat atcatctacc
    41761 atgatacatg tctttccttg aacatcaccg atcacataac caccctcagt tgaagcatca
    41821 tctgcatgat ctacgatggc taatgtgctg ttcaaatatt ctgaaagact acgtgcacgc
    41881 tgaacaccgc tgttttttgg cgaaacaact acgatgtcat ctcccgtcag acctaattga
    41941 cggtaataat gagcaaacaa cggcatcgta aataaattat ctacaggaat atcaaagaat
    42001 ccttgtactt gaaccgtgtg cagatctaaa gtcagtactc gagttgcccc tgcttcttcg
    42061 atcaagttag cgatcaattt cgcagtgatc ggttcatgag gtttcgccgt gcggtcttgc
    42121 ctagcatacc catagtaagg caatacgaca ttgatcgttt tcgcacttgc tcgcttcatt
    42181 gcatcgatca tgatcagtag ttccatatag taatcattga caggtgcatt cgttgattga
    42241 acgatataca catgatcccc tcgaatactt tcttcgatat tgatggaaat ctcgccatca
    42301 ctaaattgct tcacgacgct tttacctaat tctgttccga acactttcgc gattttttca
    42361 gctaatggac ggttgccatt caaactaaaa atcttcaacg tgtcatcctg atacttatta
    42421 ctcatgtagt cctccccaaa acttctctat tcttcgatag tttaacatat ttttccaaga
    42481 ttttctgtta atttcagcaa attcttaagg aagtgaaact gttccgttac tccaactggc
    42541 aggaatgact ttatgataat cgccccagtt gtcgtgtact ttgtaataac gttctcctgt
    42601 ttcagtttca aaataagcgt acgctgtcac ccaatgattg ccatatgtac tgccaagtac
    42661 tttcaaaata ccaatcatca caggtttgcc ttcacgtata cgttttgtag ctcgctgcca
    42721 tgaacctaga agagttgctc gtgcacgctc atgattgcca ttcttcttta aaaaactaga
    42781 aatccctgta ctaacctgaa aaggaatcgt aggaagtcct agtggttgga tctggctacg
    42841 caatgccttc gtcaaaacca acgaatcagc cgtatttttc ttgcgaatct catttggaag
    42901 catccacccc ttacgatagt cttgataata ggcaaggaga actgctgccg cgtaagtacc
    42961 acaaagatag cctgaggggg tagcccattt cttatacctc aaaaaacgtt cttctggcaa
    43021 tcccacccag caatttgcga gttcctttcc tgaccttttt tcttcgatca aaacgggaat
    43081 atcttctttt ctgttgatcc atctagataa ctcgattggc tcttcattga tcagcttcca
    43141 ttttttacaa gaaagaccct cttgataagt atattttgct ggagaatatt ctaccggatg
    43201 tcctgattga tcgataatca cataactttg atcttcaaaa accagagcta aaaaaagcaa
    43261 tttacttttt cttgactgca gctgctgagt gctggctaat tttttaattt ttttatctac
    43321 tttgaaatat aaaggtttat ccactacttg attcagttgg gatcgcctcc tttttttcat
    43381 tataagtgaa ggattgaaaa aaacaaatca gtttgattgt tcgaattttc aattttttat
    43441 gttctatttg ttaattcctt cattttgtaa gataatgggc acacgaaaca aaaaaggagc
    43501 gatcgatgaa tgaacaaaga acgattgaaa caagatttct atgaagcagt caatgaagaa
    43561 tggcaaaaaa cagcaaaaat ccctgcagat aaaccagcta caggcggttt ccaagattta
    43621 gttgacggga tcgaccaact tttgatggga gaaacagacg atctgcttgc ccatccagaa
    43681 aaagtgtctt cagaaaaaat gaaacatttc cttgcatatt atcaactggc aaacgattat
    43741 gaccgaagaa atgagttgca tgctcaaccg cttcaaccta taatcgaaaa agttgaatca
    43801 ttgcagtctt tcgacgaatt gaataagcaa tttccagaat ggctgttgga tacattacca
    43861 ttgccattta atctggatgt agatgcagat atgaagaatg cgcaaacgaa cgccttcttt
    43921 gcttatccgc cttctttatt ccttccagat aagacgtatt atgcatcaga ccatcccaac
    43981 ggtgcacaac taataggcgt tttctttgat atggctgttc agttattaga gcttgccggc
    44041 aaagaaaaaa gtcaggcaga agcaatcacg gaacaagcag tgcaatttga taaattgatt
    44101 gctcctcatg taaaaagtgc agaagaaaac gcagattaca gcaaaatgta taatccgcaa
    44161 tcttttgata catttattaa atacacagat aaactggact tgaaaaaact tgtcacgggt
    44221 ttgattggga caactccaga taaagtgatc gtaactgatc cagtgtactt cgatcatttg
    44281 aatgacatcc tgacagaagc tcattttccg ttattcaaaa gctggatgat cattaaaaca
    44341 gtacgttctc ttagtggtta tctatcagaa gaattccgcc aagtcagcgg aatctattct
    44401 cgcgtattgt ctggaacaga cgaagcaatg cctcagaaaa aagcagcgta ttaccttgcc
    44461 tctggacaat tcgatcaagt agttggcgat tactacggaa aaaaatattt tggcgaagct
    44521 gctaaaaaag acgtagaaca aatggtgaaa aaaatgatcg ccgtttataa aaaacgtcta
    44581 gaagaaaatg actggcttgg ggatgcaaca aaagaaaaag ccgtcataaa attaaataac
    44641 ttaggtatcc aagtaggcta ccccgataag atcccagcat tattcgacca gttcaaaaca
    44701 gtacctgcag atcagggtgg tactttgctt tcaaatactc ttgttttcag caaactcaca
    44761 ttaaaagatc gtttcagcaa atggaacaaa ccagtggatc gttcagaatg ggaaatgagt
    44821 gctgacacgg tcaatgctta ttatcatcca tttcggaatg tgatcgtctt tccggcagcg
    44881 atccttcaag caccatttta cagtctagaa caatcaagct ctgctaatta tggtggaatc
    44941 ggagctgtta tcgctcatga gatttcccat gcatttgata ataacggcgc tttatttgac
    45001 gaatatggca acttgaacaa ctggtggaca gaagaagatc ttacacaatt tcaaaacaaa
    45061 gcacaagcaa tgatcgaaga attcgatggt ctgccttttg ctgacggaaa agtaaacgga
    45121 aaattaaccg tttcggaaaa tattgctgat gcaggcggtc taagctgtgc attggaagca
    45181 gcaaaaacgg aatcagatct ttctatcgag gaattctttg tcaattgggc aacgatttgg
    45241 cgtacaaaag caaaaaaaga ataccaacaa cttttactgc aaatcgatgt ccatgcccct
    45301 gctaaactcc gagcaaacgt gcaaccaaaa aaccttgacg aattctatga aacatttggt
    45361 atcacggaac aagacgctat gtatctagcg ccagaaaaaa gagtgcgaat ctggtaagag
    45421 gttgtgccag aagtggtcag cttcaagaaa taagaaggaa tttgggaaaa tcgcttctca
    45481 aatttttgtg acatttcggc ttatttttcg aagaagttac ttctggaata ccatgtatta
    45541 gaggttgtga aaaagctgct tagtttcaag tagtaagaaa aattttgtga aaatggcttt
    45601 ccacattttt gcataaattt gacttactac cgagaaactg gcttttgaac accgtttatc
    45661 gagaggttgt gaaatcagcg tcctgacctg agtattagag aaaaaaacgg aaaaatagtt
    45721 cctcatattt tgccgtttgt tgagctaata ccgagggtca gctgattgaa caccgtttag
    45781 tggagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaagcag
    45841 caaggagaac ctcatctata aaaagataga aggaagtgag acatctattg gtctcatttc
    45901 cttctatttt tactttcctc agtcaattgt tttacatcat aaaaggagaa attccactta
    45961 tttttttcgc tgtttcatga cataaacaac tacaccgatt aacaaaattc ctccagctaa
    46021 tgctagcaac gggttattaa attctcccgt ttttgggagt ccttgagtgc catttttttc
    46081 actttctttt gctgtcctat ccggatgaga aggttttcca tttggttttc catttggtga
    46141 ttcatttcct ggattttctc tttctggttt gtcatttccc gggggcacat cggcagtttc
    46201 tcgcacagtg atcgcttttt gctgggtaac agattctgta tgagcagacc atttttcccc
    46261 atcaaatatt tccgaacgat aagtgacttc tagcgtatat tctccttttg aaggtgctga
    46321 aaaaatagcg gtatgattag aaaattgagc acttgactgt gtttcaacga tttggatctt
    46381 ttctgggaaa taacgcatat cttcaaagac aggcgttcct tgatttggtg tgaaagttga
    46441 cacaaaaact gtaaaaggct tcagttcaat gatatttcct tctgcattct ccgccacacc
    46501 atttgcttct tgtataataa tcggattatt ttgttcattt ggttttgtct gatttggttc
    46561 ttgtacgatt gcatactctg gatattccga agacggtgag gtttctccgt caaaaatagc
    46621 acctacagat aatggaagac catccactct ttccttttct gtttgacgat tccccggttt
    46681 aatctgcatc agtccatgaa catggatact tccattttca tagcgcgtaa caggctgctg
    46741 accttctttc gaacatgtat ttagagactc gatccaatct tttgatatgg tatgtccaag
    46801 agtattttct gattgtttct ctcgttcatt ccaatagtag ttcaacgagt ggtagatttg
    46861 atcttcgttt tgattcaatg ggcggatctg ctctctcatc tcaagattct ctgttcggaa
    46921 agataagata ccagaagcat ggtagtctgt gtttcctgct gaagaggtat aaagagccac
    46981 attatatgaa ccgttattaa aacttgtact gctattgacg ataatatctg ttccgctatt
    47041 ggaatcgatt cctttcgcaa gattttcgaa agacaaacta ttgatcaatt catgagggcc
    47101 cgttaaagaa gaaccgccca ttttgaatcc attaccgtta ccttctccct caattcctgg
    47161 tacccagccg tttcgataag caactgagtt tttgatgatt acttttccga tcgaacctgt
    47221 ttcattttta gcaaaaaggt cccaaccgtc atctgcatta tgatacgcga tacaaccgtc
    47281 aaatacattt ccttctccta ttgtcagttt ggccgcaaat ccatcggcat cttcaaatcc
    47341 tggatcggca ttttcaaaag atgtacagtt cttgactaag ttatgcgcgg gccatgcttc
    47401 gaaaggatca tttgagctac cgctgatttg gattcctgta tttccattcc gataagcatg
    47461 aatctcttcg atcaaattat gatttccaga aatctgtaat cctttttgca tagctgcact
    47521 atttgtaatg tcaaaccctc gtaaacccca gtagtgactg aacagtgtca tcccatttcc
    47581 agttccttta aaatcaagta caggacgctt gttttctgga tttttctctg caatcaactg
    47641 aatcgggttt tctcttgtcc cgttaacgcc ttttggaatc gtcaatcctc tttcgaagga
    47701 ataagttcca ggcgccatga cgattgtctg cccagcatat gcgtatctca atgcttgtgt
    47761 cagagataat ggattttgtc tgcttccgtt tcctgcatct gacccttctg gtgttacata
    47821 aactgtcgaa tatttaggtt tacggtagtc cactgtatga agaatctctt ttgtttcata
    47881 actagtcatc tctacgtatt cttctggcgg atagttttta tctggcgtaa aactgaaagt
    47941 aaattcgttc gttccttcca tcagttttgt ctgataatca aattcccgat tcgcagtgat
    48001 agccaagttt tttgcgttct taagcacctc tcctgttgct ttatttttaa ttgtcaataa
    48061 tccgtcagca tttgctctaa aggacatagt atgatcttgg tttcctgaat gattactaga
    48121 ggtcacagtg taaatcggat caatcaagcg agtaggtctt tcttgcccag gaggatctgt
    48181 cgcaggatct gagtaagcaa cttcgattcc ttctaccttg atcgtcatat tccttgctgc
    48241 agcaaatcct gcataaataa ccgattcatc tgaagcgtat aatttttccc agtcatacat
    48301 gatgtcagaa gccatttctt ctcctgtttc aggatcgtaa taatatgcct ctaaaccagt
    48361 attcgttttt ttcatctcta cgtccagttt agtaatcgag ttcgtggccg gaaacagatt
    48421 cccatgaccg tttccgaata tattataaga acctgtttct ttttccaaga accctgctga
    48481 agtctctaaa ggtgttgttt ccactgagac actcccagga gctggcgcag cttgcggatc
    48541 ttcagatgat attcctgtga ttgcccgtgt ccccaatcca agtcgcatcg tatatttgct
    48601 gccgctcgtg ttgaccactt cttgtctatc cgagtcccag acatattcga tccgactgcc
    48661 tagtatggca aatgaatttg aataaaatgc ctgcccaaac aaatgatttt ttggtactgc
    48721 gtctcttacc attaaagcaa atccttcttg ggcattcgta taggtccaag attctatttc
    48781 gatcgtcgcc cgcatcctaa agttgatgtc tttggggatt gcttgataat aataggtcat
    48841 tccatctgat ccgcttgact ggaattttcc gccgttattc gtggaagaga gccgcacgcc
    48901 gtcttctaat ggttcaatgg catacctact agcacttgtg ctttctccga aaacaacccc
    48961 tttccactct tcatttgctt cttcagattg cttttttgtc ttttcatttt ctgattcttt
    49021 catcatagga tcatttcctt cttccgtatc catatcttca ctagttcttg gtatttcctc
    49081 cattgttgac atcccttcaa tgttcacgtg tgcattttct tcgttcacag actgttgttc
    49141 gttagcagca acttcaacga cagaaattaa aaccggggta ctgagcataa ggagattcat
    49201 cattagcgca ctgtatttta tacgtttttt taacaagtgt attcctcccg taacaaatta
    49261 gaaagcgttt acttaaagta ttacctaatt tatttttact gtcaataaaa aacaaaatta
    49321 taataagtgt attttataaa aagaaaaaat ataacttaag ttgttgataa taaagagatt
    49381 tattaacgta aaaagaataa gtttttttaa aataacgtat a
//
LOCUS       DAZUJO010000013        44828 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000013 DAZUJO010000000
VERSION     DAZUJO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.013"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1008)
                     /locus_tag="ACG8OL_000824"
     CDS             complement(<1..1008)
                     /locus_tag="ACG8OL_000824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4816830.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1188..1532)
                     /locus_tag="ACG8OL_000825"
     CDS             complement(1188..1532)
                     /locus_tag="ACG8OL_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4816831.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1525..1959)
                     /locus_tag="ACG8OL_000826"
     CDS             complement(1525..1959)
                     /locus_tag="ACG8OL_000826"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS4816832.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2097..2834
                     /locus_tag="ACG8OL_000827"
     CDS             2097..2834
                     /locus_tag="ACG8OL_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4816833.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2827..4038
                     /locus_tag="ACG8OL_000828"
     CDS             2827..4038
                     /locus_tag="ACG8OL_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4816834.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4242..5024
                     /locus_tag="ACG8OL_000829"
     CDS             4242..5024
                     /locus_tag="ACG8OL_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS4816835.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5094..5756
                     /gene="trmB"
                     /locus_tag="ACG8OL_000830"
     CDS             5094..5756
                     /gene="trmB"
                     /locus_tag="ACG8OL_000830"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS4816836.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5854..7608)
                     /gene="ade"
                     /locus_tag="ACG8OL_000831"
     CDS             complement(5854..7608)
                     /gene="ade"
                     /locus_tag="ACG8OL_000831"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS4816837.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7740..8669
                     /gene="comGA"
                     /locus_tag="ACG8OL_000832"
     CDS             7740..8669
                     /gene="comGA"
                     /locus_tag="ACG8OL_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS4816838.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8626..9675
                     /gene="comGB"
                     /locus_tag="ACG8OL_000833"
     CDS             8626..9675
                     /gene="comGB"
                     /locus_tag="ACG8OL_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS4816839.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9672..9950
                     /gene="comGC"
                     /locus_tag="ACG8OL_000834"
     CDS             9672..9950
                     /gene="comGC"
                     /locus_tag="ACG8OL_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS4816840.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9925..10395
                     /gene="comGD"
                     /locus_tag="ACG8OL_000835"
     CDS             9925..10395
                     /gene="comGD"
                     /locus_tag="ACG8OL_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS4816841.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10367..10714
                     /locus_tag="ACG8OL_000836"
     CDS             10367..10714
                     /locus_tag="ACG8OL_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816842.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10677..11102
                     /gene="comGF"
                     /locus_tag="ACG8OL_000837"
     CDS             10677..11102
                     /gene="comGF"
                     /locus_tag="ACG8OL_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS4816843.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11074..11433
                     /gene="comGG"
                     /locus_tag="ACG8OL_000838"
     CDS             11074..11433
                     /gene="comGG"
                     /locus_tag="ACG8OL_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS4816844.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11605..12612
                     /locus_tag="ACG8OL_000839"
     CDS             11605..12612
                     /locus_tag="ACG8OL_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4816845.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12637..13821
                     /locus_tag="ACG8OL_000840"
     CDS             12637..13821
                     /locus_tag="ACG8OL_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS4816846.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="ACG8OL_000841"
     CDS             complement(14258..15841)
                     /gene="abc-f"
                     /locus_tag="ACG8OL_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS4816847.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15946..16074)
                     /locus_tag="ACG8OL_000842"
     CDS             complement(15946..16074)
                     /locus_tag="ACG8OL_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816848.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16419..16562)
                     /locus_tag="ACG8OL_000843"
     CDS             complement(16419..16562)
                     /locus_tag="ACG8OL_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS4816849.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16785..17264)
                     /locus_tag="ACG8OL_000844"
     CDS             complement(16785..17264)
                     /locus_tag="ACG8OL_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4816850.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17337..17621)
                     /locus_tag="ACG8OL_000845"
     CDS             complement(17337..17621)
                     /locus_tag="ACG8OL_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816851.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17699..17893)
                     /gene="ssrS"
                     /locus_tag="ACG8OL_000846"
     ncRNA           complement(17699..17893)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8OL_000846"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17965..19251)
                     /locus_tag="ACG8OL_000847"
     CDS             complement(17965..19251)
                     /locus_tag="ACG8OL_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS4816852.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19389..20060
                     /locus_tag="ACG8OL_000848"
     CDS             19389..20060
                     /locus_tag="ACG8OL_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS4816853.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20026..20514
                     /locus_tag="ACG8OL_000849"
     CDS             20026..20514
                     /locus_tag="ACG8OL_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS4816854.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20527..21474
                     /gene="prmA"
                     /locus_tag="ACG8OL_000850"
     CDS             20527..21474
                     /gene="prmA"
                     /locus_tag="ACG8OL_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS4816855.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21475..22239
                     /locus_tag="ACG8OL_000851"
     CDS             21475..22239
                     /locus_tag="ACG8OL_000851"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS4816856.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22472..24685
                     /locus_tag="ACG8OL_000852"
     CDS             22472..24685
                     /locus_tag="ACG8OL_000852"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS4816857.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24696..25142
                     /gene="dtd"
                     /locus_tag="ACG8OL_000853"
     CDS             24696..25142
                     /gene="dtd"
                     /locus_tag="ACG8OL_000853"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS4816858.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25313..26572
                     /locus_tag="ACG8OL_000854"
     CDS             25313..26572
                     /locus_tag="ACG8OL_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS4816859.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26661..27608)
                     /locus_tag="ACG8OL_000855"
     CDS             complement(26661..27608)
                     /locus_tag="ACG8OL_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4816860.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28002..29294
                     /gene="hisS"
                     /locus_tag="ACG8OL_000856"
     CDS             28002..29294
                     /gene="hisS"
                     /locus_tag="ACG8OL_000856"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS4816861.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29309..31078
                     /gene="aspS"
                     /locus_tag="ACG8OL_000857"
     CDS             29309..31078
                     /gene="aspS"
                     /locus_tag="ACG8OL_000857"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS4816862.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31187..32062
                     /locus_tag="ACG8OL_000858"
     CDS             31187..32062
                     /locus_tag="ACG8OL_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4816863.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32328..34211
                     /locus_tag="ACG8OL_000859"
     CDS             32328..34211
                     /locus_tag="ACG8OL_000859"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS4816864.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34286..34570)
                     /locus_tag="ACG8OL_000860"
     CDS             complement(34286..34570)
                     /locus_tag="ACG8OL_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4816865.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34560..35294)
                     /locus_tag="ACG8OL_000861"
     CDS             complement(34560..35294)
                     /locus_tag="ACG8OL_000861"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS4816866.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35423..36046
                     /locus_tag="ACG8OL_000862"
     CDS             35423..36046
                     /locus_tag="ACG8OL_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS4816867.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36238..37359
                     /locus_tag="ACG8OL_000863"
     CDS             36238..37359
                     /locus_tag="ACG8OL_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4816868.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37366..40494
                     /locus_tag="ACG8OL_000864"
     CDS             37366..40494
                     /locus_tag="ACG8OL_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4816869.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40513..43707
                     /locus_tag="ACG8OL_000865"
     CDS             40513..43707
                     /locus_tag="ACG8OL_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS4816870.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43853..44560)
                     /locus_tag="ACG8OL_000866"
     CDS             complement(43853..44560)
                     /locus_tag="ACG8OL_000866"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4816871.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14380 a         7959 c         9408 g        13081 t
ORIGIN      
        1 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
       61 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
      121 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
      181 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
      241 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
      301 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
      361 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
      421 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
      481 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
      541 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
      601 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
      661 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
      721 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
      781 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
      841 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
      901 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
      961 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     1021 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     1081 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     1141 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     1201 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     1261 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     1321 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     1381 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     1441 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     1501 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     1561 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     1621 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     1681 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     1741 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     1801 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     1861 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     1921 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     1981 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     2041 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     2101 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     2161 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     2221 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     2281 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     2341 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     2401 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     2461 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     2521 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     2581 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     2641 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     2701 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     2761 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     2821 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     2881 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     2941 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     3001 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     3061 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     3121 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     3181 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     3241 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     3301 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     3361 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     3421 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     3481 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     3541 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     3601 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     3661 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     3721 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     3781 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     3841 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     3901 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     3961 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     4021 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     4081 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     4141 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     4201 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     4261 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     4321 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     4381 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     4441 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     4501 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     4561 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     4621 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     4681 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     4741 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     4801 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     4861 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     4921 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     4981 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     5041 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     5101 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     5161 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     5221 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     5281 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     5341 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     5401 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     5461 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     5521 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     5581 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     5641 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     5701 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     5761 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     5821 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     5881 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     5941 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     6001 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     6061 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     6121 attgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     6181 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     6241 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     6301 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     6361 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     6421 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     6481 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     6541 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     6601 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     6661 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     6721 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     6781 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     6841 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     6901 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     6961 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     7021 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     7081 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     7141 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     7201 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     7261 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     7321 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     7381 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     7441 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     7501 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     7561 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     7621 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     7681 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     7741 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     7801 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     7861 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     7921 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     7981 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     8041 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     8101 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     8161 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     8221 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     8281 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     8341 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     8401 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     8461 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     8521 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     8581 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     8641 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     8701 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     8761 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     8821 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     8881 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     8941 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     9001 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     9061 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     9121 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     9181 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     9241 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     9301 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     9361 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     9421 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     9481 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     9541 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     9601 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     9661 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     9721 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     9781 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     9841 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     9901 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     9961 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    10021 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    10081 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    10141 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    10201 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    10261 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    10321 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    10381 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    10441 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    10501 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    10561 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    10621 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    10681 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    10741 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    10801 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    10861 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    10921 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    10981 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    11041 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    11101 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    11161 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    11221 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    11281 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    11341 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    11401 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    11461 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    11521 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    11581 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    11641 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    11701 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    11761 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    11821 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    11881 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    11941 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    12001 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    12061 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    12121 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    12181 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    12241 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    12301 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    12361 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    12421 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    12481 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    12541 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    12601 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    12661 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    12721 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    12781 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    12841 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    12901 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    12961 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    13021 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    13081 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    13141 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    13201 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    13261 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    13321 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    13381 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    13441 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    13501 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    13561 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    13621 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    13681 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    13741 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    13801 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    13861 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    13921 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    13981 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    14041 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    14101 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    14161 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    14221 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    14281 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    14341 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    14401 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    14461 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    14521 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    14581 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    14641 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    14701 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    14761 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    14821 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    14881 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    14941 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    15001 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    15061 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    15121 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    15181 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    15241 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    15301 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    15361 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    15421 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    15481 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    15541 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    15601 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    15661 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    15721 tgccagttcc gttgataccg actaaggcaa ttttttcacc agcttcaatt tgacaggata
    15781 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    15841 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    15901 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    15961 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    16021 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    16081 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    16141 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    16201 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    16261 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    16321 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    16381 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    16441 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    16501 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    16561 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    16621 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    16681 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    16741 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    16801 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    16861 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    16921 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    16981 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    17041 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    17101 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    17161 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    17221 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    17281 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    17341 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    17401 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    17461 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    17521 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    17581 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    17641 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    17701 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    17761 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    17821 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    17881 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    17941 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    18001 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    18061 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    18121 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    18181 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    18241 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    18301 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    18361 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    18421 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    18481 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    18541 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    18601 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    18661 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    18721 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    18781 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    18841 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    18901 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    18961 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    19021 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    19081 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    19141 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    19201 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    19261 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    19321 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    19381 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    19441 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    19501 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    19561 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    19621 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    19681 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    19741 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    19801 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    19861 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    19921 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    19981 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    20041 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    20101 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    20161 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    20221 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    20281 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    20341 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    20401 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    20461 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    20521 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    20581 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    20641 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    20701 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    20761 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    20821 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    20881 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    20941 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    21001 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    21061 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    21121 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    21181 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    21241 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    21301 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    21361 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    21421 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    21481 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    21541 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    21601 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    21661 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    21721 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    21781 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    21841 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    21901 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    21961 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    22021 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    22081 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    22141 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    22201 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    22261 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    22321 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    22381 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    22441 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    22501 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    22561 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    22621 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    22681 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    22741 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    22801 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    22861 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    22921 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    22981 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    23041 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    23101 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    23161 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    23221 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    23281 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    23341 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    23401 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    23461 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    23521 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    23581 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    23641 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    23701 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    23761 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    23821 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    23881 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    23941 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    24001 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    24061 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    24121 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    24181 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    24241 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    24301 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    24361 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    24421 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    24481 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    24541 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    24601 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    24661 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    24721 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    24781 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    24841 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    24901 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    24961 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    25021 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    25081 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    25141 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    25201 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    25261 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    25321 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    25381 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    25441 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    25501 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    25561 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    25621 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    25681 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    25741 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    25801 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    25861 aggtgtcgag aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    25921 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    25981 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    26041 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    26101 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    26161 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    26221 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    26281 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    26341 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    26401 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    26461 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    26521 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    26581 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    26641 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    26701 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    26761 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    26821 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    26881 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    26941 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    27001 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    27061 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    27121 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    27181 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    27241 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    27301 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    27361 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    27421 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    27481 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    27541 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    27601 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    27661 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    27721 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    27781 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    27841 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    27901 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    27961 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    28021 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    28081 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    28141 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    28201 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    28261 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    28321 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    28381 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    28441 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    28501 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    28561 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    28621 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    28681 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    28741 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    28801 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    28861 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    28921 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    28981 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    29041 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    29101 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    29161 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    29221 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    29281 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    29341 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    29401 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    29461 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    29521 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    29581 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    29641 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    29701 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    29761 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    29821 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    29881 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    29941 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    30001 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    30061 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    30121 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    30181 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    30241 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    30301 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    30361 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    30421 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    30481 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    30541 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    30601 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    30661 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    30721 tgcagaagct tatgatatcg tcttgaacgg ttatgaatta ggcggaggtt ctatccgtat
    30781 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    30841 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    30901 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    30961 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    31021 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    31081 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    31141 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    31201 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    31261 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    31321 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    31381 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    31441 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    31501 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    31561 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    31621 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    31681 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    31741 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    31801 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    31861 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    31921 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    31981 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    32041 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    32101 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    32161 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    32221 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    32281 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    32341 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    32401 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    32461 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    32521 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    32581 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    32641 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    32701 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    32761 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    32821 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    32881 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    32941 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    33001 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    33061 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    33121 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    33181 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    33241 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    33301 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    33361 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    33421 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    33481 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    33541 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    33601 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    33661 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    33721 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    33781 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    33841 taaaggaaca agatttgttg aaagacactg ttctgattgg actaggaacg aatggctcat
    33901 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    33961 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    34021 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    34081 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    34141 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    34201 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    34261 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    34321 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    34381 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    34441 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    34501 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    34561 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    34621 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    34681 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    34741 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    34801 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    34861 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    34921 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    34981 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    35041 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    35101 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    35161 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    35221 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    35281 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    35341 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    35401 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    35461 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    35521 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    35581 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    35641 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    35701 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    35761 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    35821 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    35881 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    35941 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    36001 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    36061 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    36121 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    36181 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    36241 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    36301 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    36361 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    36421 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    36481 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    36541 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    36601 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    36661 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    36721 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    36781 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    36841 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    36901 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    36961 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    37021 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    37081 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    37141 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    37201 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    37261 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    37321 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    37381 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    37441 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    37501 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    37561 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    37621 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    37681 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    37741 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    37801 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    37861 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    37921 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    37981 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    38041 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    38101 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    38161 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    38221 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    38281 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    38341 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    38401 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    38461 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    38521 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    38581 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    38641 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    38701 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    38761 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    38821 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    38881 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    38941 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    39001 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    39061 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    39121 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    39181 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    39241 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    39301 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    39361 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    39421 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    39481 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    39541 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    39601 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    39661 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    39721 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    39781 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    39841 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    39901 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    39961 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    40021 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    40081 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    40141 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    40201 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    40261 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    40321 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    40381 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    40441 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    40501 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    40561 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    40621 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    40681 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    40741 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    40801 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    40861 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    40921 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    40981 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    41041 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    41101 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    41161 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    41221 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    41281 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    41341 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    41401 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    41461 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    41521 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    41581 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    41641 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggt
    41701 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    41761 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    41821 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    41881 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    41941 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    42001 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    42061 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    42121 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    42181 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    42241 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    42301 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    42361 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    42421 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    42481 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    42541 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    42601 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    42661 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    42721 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    42781 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    42841 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    42901 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    42961 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    43021 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    43081 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    43141 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    43201 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    43261 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    43321 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    43381 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    43441 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    43501 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    43561 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    43621 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    43681 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    43741 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    43801 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    43861 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    43921 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    43981 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    44041 tatgaaattc tttctcagcc agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    44101 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    44161 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    44221 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    44281 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    44341 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    44401 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    44461 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    44521 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    44581 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    44641 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    44701 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga acaaattaat
    44761 cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt ttcgaaagat
    44821 ttattttt
//
LOCUS       DAZUJO010000014        43651 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000014 DAZUJO010000000
VERSION     DAZUJO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.014"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..907)
                     /locus_tag="ACG8OL_000867"
     CDS             complement(32..907)
                     /locus_tag="ACG8OL_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4816872.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1055..1387
                     /locus_tag="ACG8OL_000868"
     CDS             1055..1387
                     /locus_tag="ACG8OL_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4816873.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1499..2707
                     /locus_tag="ACG8OL_000869"
     CDS             1499..2707
                     /locus_tag="ACG8OL_000869"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS4816874.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2861..3316
                     /locus_tag="ACG8OL_000870"
     CDS             2861..3316
                     /locus_tag="ACG8OL_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816875.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3678..3953
                     /locus_tag="ACG8OL_000871"
     CDS             3678..3953
                     /locus_tag="ACG8OL_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816876.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3966..6044
                     /locus_tag="ACG8OL_000872"
     CDS             3966..6044
                     /locus_tag="ACG8OL_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS4816877.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            6207..8090
                     /locus_tag="ACG8OL_000873"
     CDS             6207..8090
                     /locus_tag="ACG8OL_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4816878.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            8102..9049
                     /locus_tag="ACG8OL_000874"
     CDS             8102..9049
                     /locus_tag="ACG8OL_000874"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS4816879.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            9069..9596
                     /locus_tag="ACG8OL_000875"
     CDS             9069..9596
                     /locus_tag="ACG8OL_000875"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4816880.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(9659..10312)
                     /locus_tag="ACG8OL_000876"
     CDS             complement(9659..10312)
                     /locus_tag="ACG8OL_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS4816881.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            10444..11286
                     /locus_tag="ACG8OL_000877"
     CDS             10444..11286
                     /locus_tag="ACG8OL_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS4816882.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11444..12340
                     /locus_tag="ACG8OL_000878"
     CDS             11444..12340
                     /locus_tag="ACG8OL_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4816883.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            12343..13056
                     /locus_tag="ACG8OL_000879"
     CDS             12343..13056
                     /locus_tag="ACG8OL_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS4816884.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            13076..13594
                     /gene="msrA"
                     /locus_tag="ACG8OL_000880"
     CDS             13076..13594
                     /gene="msrA"
                     /locus_tag="ACG8OL_000880"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4816885.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            13591..13818
                     /locus_tag="ACG8OL_000881"
     CDS             13591..13818
                     /locus_tag="ACG8OL_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS4816886.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            13970..14521
                     /gene="lepB"
                     /locus_tag="ACG8OL_000882"
     CDS             13970..14521
                     /gene="lepB"
                     /locus_tag="ACG8OL_000882"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4816887.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(14604..15146)
                     /locus_tag="ACG8OL_000883"
     CDS             complement(14604..15146)
                     /locus_tag="ACG8OL_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4816888.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(15383..15781)
                     /locus_tag="ACG8OL_000884"
     CDS             complement(15383..15781)
                     /locus_tag="ACG8OL_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4816889.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            16041..16901
                     /gene="ylqF"
                     /locus_tag="ACG8OL_000885"
     CDS             16041..16901
                     /gene="ylqF"
                     /locus_tag="ACG8OL_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS4816890.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            16894..17661
                     /locus_tag="ACG8OL_000886"
     CDS             16894..17661
                     /locus_tag="ACG8OL_000886"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS4816891.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            17717..18574
                     /gene="dprA"
                     /locus_tag="ACG8OL_000887"
     CDS             17717..18574
                     /gene="dprA"
                     /locus_tag="ACG8OL_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS4816892.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            18696..20774
                     /gene="topA"
                     /locus_tag="ACG8OL_000888"
     CDS             18696..20774
                     /gene="topA"
                     /locus_tag="ACG8OL_000888"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS4816893.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            20743..22119
                     /gene="trmFO"
                     /locus_tag="ACG8OL_000889"
     CDS             20743..22119
                     /gene="trmFO"
                     /locus_tag="ACG8OL_000889"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS4816894.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            22317..23222
                     /gene="xerC"
                     /locus_tag="ACG8OL_000890"
     CDS             22317..23222
                     /gene="xerC"
                     /locus_tag="ACG8OL_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS4816895.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            23259..23807
                     /gene="hslV"
                     /locus_tag="ACG8OL_000891"
     CDS             23259..23807
                     /gene="hslV"
                     /locus_tag="ACG8OL_000891"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS4816896.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            23821..25221
                     /gene="hslU"
                     /locus_tag="ACG8OL_000892"
     CDS             23821..25221
                     /gene="hslU"
                     /locus_tag="ACG8OL_000892"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS4816897.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            25239..26030
                     /gene="codY"
                     /locus_tag="ACG8OL_000893"
     CDS             25239..26030
                     /gene="codY"
                     /locus_tag="ACG8OL_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS4816898.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            26112..26987
                     /locus_tag="ACG8OL_000894"
     CDS             26112..26987
                     /locus_tag="ACG8OL_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS4816899.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="ACG8OL_000895"
     CDS             complement(27119..27757)
                     /gene="plsY"
                     /locus_tag="ACG8OL_000895"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS4816900.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            27884..28318
                     /locus_tag="ACG8OL_000896"
     CDS             27884..28318
                     /locus_tag="ACG8OL_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS4816901.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            28484..30529
                     /gene="parE"
                     /locus_tag="ACG8OL_000897"
     CDS             28484..30529
                     /gene="parE"
                     /locus_tag="ACG8OL_000897"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS4816902.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            30541..32991
                     /gene="parC"
                     /locus_tag="ACG8OL_000898"
     CDS             30541..32991
                     /gene="parC"
                     /locus_tag="ACG8OL_000898"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS4816903.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            33268..35499
                     /gene="pflB"
                     /locus_tag="ACG8OL_000899"
     CDS             33268..35499
                     /gene="pflB"
                     /locus_tag="ACG8OL_000899"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS4816904.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            35743..36504
                     /gene="pflA"
                     /locus_tag="ACG8OL_000900"
     CDS             35743..36504
                     /gene="pflA"
                     /locus_tag="ACG8OL_000900"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS4816905.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            36589..37515
                     /locus_tag="ACG8OL_000901"
     CDS             36589..37515
                     /locus_tag="ACG8OL_000901"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS4816906.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            37697..38590
                     /locus_tag="ACG8OL_000902"
     CDS             37697..38590
                     /locus_tag="ACG8OL_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS4816907.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            38724..38849
                     /locus_tag="ACG8OL_000903"
     CDS             38724..38849
                     /locus_tag="ACG8OL_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS4816908.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(38983..39615)
                     /locus_tag="ACG8OL_000904"
     CDS             complement(38983..39615)
                     /locus_tag="ACG8OL_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816909.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(39763..40305)
                     /locus_tag="ACG8OL_000905"
     CDS             complement(39763..40305)
                     /locus_tag="ACG8OL_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS4816910.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            40440..40829
                     /locus_tag="ACG8OL_000906"
     CDS             40440..40829
                     /locus_tag="ACG8OL_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816911.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            40833..41597
                     /locus_tag="ACG8OL_000907"
     CDS             40833..41597
                     /locus_tag="ACG8OL_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4816912.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            41726..42673
                     /locus_tag="ACG8OL_000908"
     CDS             41726..42673
                     /locus_tag="ACG8OL_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4816913.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            42803..43543
                     /locus_tag="ACG8OL_000909"
     CDS             42803..43543
                     /locus_tag="ACG8OL_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816914.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT        15197 a         7497 c         9136 g        11821 t
ORIGIN      
        1 ctttcctcat agcctatctt tgtctttttt cttatagttt tttcagtaat cttgtctgtg
       61 gcttcaaata agtaaagcct tgcccttcca cttcatgcac attgatgatt gaaaggaaag
      121 ctttttcgtc aatctcgtgg acgatcctct tagcttcagt taattcacta gagctaacga
      181 ccatatagat gatttttttt gaattaccag agaatgcgcc ttctccagaa aggaaagtca
      241 cacctctttg cagaccggtc atcagtagtg gtgcaatctc ttcgcttttg ttggagacga
      301 caagtagacc ttttgcagaa tatccgccat ctaagatcga atcaatcaca cgactgaaaa
      361 catacgaaac aatcaacgta tacatcatac gtttcaagtc gatataggtt aatgaagcaa
      421 gtaagacgat gatatcaaat gccaataggg aacgccccat agtgatgccg taattttgtt
      481 ctaaaatccg agcgataata tcactgccgc ctgttgttcc acccatgcgg tagacgatcc
      541 cgcttcctac acctgcaact aaacccgcaa gtagagaaac gatcagtaga tcatgctgta
      601 aattgatttc tagtggaacc ctttgccaga tccataaaaa gaatgacaaa gaaagtgtac
      661 ctagaacagt ataggccaag gaacgtttac cgaggatctt tcctcctagt aaaatcaatg
      721 ggatgttgat taataaagtc gtataagctg gatcaatacc aaataaagct cttaagatca
      781 aggtgatccc tgagactccc ccttctgcta aatgattagc gatgttcaag taaacaagac
      841 caaatgcata aatacttgtt cctataatga taaaaagaac ttcttttact gtttttcgtt
      901 tgaccatatg aattccagct ttcaattatg tattttcaca gaatttccat ttagaaattc
      961 cttcctaaat gtagcatgga aattcgattt tctcaattgc tcttatctag gatttgcgtt
     1021 aaagttttgg taagatagtt ttggagggaa aaagatggat aaaaagcgtt ctttatacag
     1081 tatgcagcag gaagtggatg aatatatcca gcaattcaag gtggggtatt tctctccatt
     1141 agctcagatg gctcgattaa ctgaagaagt aggtgaattg gcaagagaag tcaaccacac
     1201 ttatggagaa aaaagcaaaa aggcttctga accagttaac tcggtagcag aagaacttgg
     1261 tgatgtttta ttcgttacga tgatcatggc taattcattg aatatcgact taactgaagt
     1321 ttttgaaaaa aacatggaaa aattcaatca aagagaccat aatcgctttg agcgtaagga
     1381 cgagtaaaca agatgaagtg gtcctggcct tgaagactct gaaacggact tgtcaaacag
     1441 tcaccatgca actgagtctt tatctcagga caagaaacac gcaggaaagg ttggaaaaat
     1501 gaaattaaaa caattacctc tggaatacca aaaggcaatt ccagttctga aaaaattaga
     1561 gaatgcagga tatgaagctt attttgtagg tggaagtgtt cgtgatatct tgctgaacca
     1621 gccaatccat gatgtggata tcgcaacaag cgcatttcca gaagaaatca aacagctttt
     1681 tccaaaaacg atagatattg ggatcgaaca tggaactgtg ttggtattag aaaatgatga
     1741 acaatatgag atcaccacat ttcggacgga atcaacttat caggactacc gtagaccaga
     1801 tcatgtcgaa ttcgttcgtt cgttggcaga agatttgaaa agaagagatt ttacgatcaa
     1861 tgcgtttgct ttgaaagaag atggagaaat catcgatttg ttcgatggtc tgcaagatct
     1921 agaagaacag atcttgcgag ctgtaggcaa tcctcatgag cggttccatg aagatgcctt
     1981 acgaatgatg cgcgggttgc gttttgtcag tcaactaggg ttcaaattag aggaagaaac
     2041 cttctacgcc atcaaagaaa accacgagct attaggtaaa atatctgtag aacgtataaa
     2101 tgtggaattc atcaagatgc tcttgggaaa tgaacgggcc gcgggaatcc atgcatttat
     2161 tgaaacagag tgctacattt gctgtcccgg tttgaaagaa gaaggcgaag cacttttacg
     2221 ttttgctgaa ttaccatccg tcccattgaa agaagagagc caagcatggg cattattgat
     2281 cagccaactg gatctaaaag aaaatgagat tcgttccttt ttgaaatcgt ggaagtgttc
     2341 taaccaaatg attaaagacg ttcaagcact tgtccaagga ctacgacaac gagaaactgg
     2401 agctttggaa ccattcgaat tgtacttctt aggcaaaaaa catgcactgc aagtagaaga
     2461 attgatgatc tattttgacg aaacacctca gatggatgcc gtaggagaat cctatgatcg
     2521 tttacctatc aagtcattaa gcgaattagc agtagatggt aaaatcttgc tagaagaaac
     2581 tggaaaaaaa cctggtaagt gggttagtga agctttgcaa ttagctgaaa aagccgtcgt
     2641 agaacaaaga ataaaaaatg ataaaaaaca ggttttggat tttttatcga aagaaaaatt
     2701 gatttaaaaa acactagact gtatctgctc agctttgttg gctacttaaa gtgggcagat
     2761 acgtcattag aagaaaaaac ataagatgtg accacttaca taaatttgtg ctaaactgag
     2821 gtagtacaag aataaattac atcataaagg agtcttttta atgagtaaag aaatgacagc
     2881 gctaaaattt tattttagaa atggagaaac ctggacgatc aaccgccgtc atatcggcga
     2941 tctttggatc aaacaaatca caacaagttt tggacgaatc aatggcagtg aatttgttga
     3001 aatccatccg tgtgctggat ttaaaatcga aatcttccat gaaggggacg cagttgcgac
     3061 acatgatatc aacctaggcg gactggaaat gggcatgttt aatcgcgcat tgaaatacga
     3121 agatatcgag cgaatggaaa tcctttaccg aaatggcaca cctgatcttg tgtacttccc
     3181 atatctagat aaaggaacag aaggattaga caatcaatat caatcaacaa aaatcagcga
     3241 aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa cgtgtagaag atgtatacgg
     3301 ggaatttttc gaataaatcg atttttacgc aaaaatgagg ctgtgaaaga gatgtgtctt
     3361 attactactg ataggctgtc tcttccacac cgtttattca gaataaggga ctgtgacaag
     3421 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
     3481 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
     3541 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
     3601 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
     3661 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
     3721 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
     3781 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
     3841 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
     3901 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
     3961 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
     4021 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
     4081 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
     4141 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
     4201 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
     4261 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
     4321 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
     4381 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     4441 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     4501 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     4561 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     4621 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     4681 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     4741 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     4801 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     4861 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     4921 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     4981 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     5041 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     5101 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     5161 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     5221 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     5281 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     5341 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     5401 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     5461 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     5521 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     5581 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     5641 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     5701 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     5761 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     5821 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     5881 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     5941 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     6001 ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc
     6061 aagttttata aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg
     6121 agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac
     6181 aatatccaag ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa
     6241 cttatggtga aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta
     6301 tcgggttgat tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta
     6361 tcgatagcag cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt
     6421 atctttctca agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag
     6481 gaaacagccc cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg
     6541 aaaacggaga agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata
     6601 aagaagatgc ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca
     6661 gtgaattaga taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg
     6721 cgcaagtgtt gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag
     6781 attatgaagc gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga
     6841 tggttaccca cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat
     6901 ttggaaaatt atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac
     6961 gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag
     7021 cttggatgag ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt
     7081 tcgaagatct aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc
     7141 tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa
     7201 atgaccaaac actgctagaa catttagatt tattgattca atctcgggaa agactaggta
     7261 ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc
     7321 ccctggatag cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag
     7381 aaaatgaaga aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag
     7441 aagcaaaaac agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat
     7501 ttccaagatt tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt
     7561 tatttttgtt gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca
     7621 atgatttgga catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg
     7681 ctgtgatcac tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag
     7741 tattccaagg aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga
     7801 agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac
     7861 ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg
     7921 agattgcaga acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag
     7981 ataatttcac tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg
     8041 aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag
     8101 gatggaacaa gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga
     8161 agacagaaca ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca
     8221 aaaagggttt cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt
     8281 gctttggttt ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat
     8341 ttgggatgaa tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat
     8401 gactgatttt ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca
     8461 gagcaagctt ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga
     8521 tcttggacac atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat
     8581 cgatcaaatc aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat
     8641 cgtttctgca tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat
     8701 gtttcaattt tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga
     8761 tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc
     8821 acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta
     8881 tatcaatcat atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac
     8941 tttagttttg aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt
     9001 agaaggatat gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg
     9061 atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag
     9121 accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa
     9181 atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc
     9241 gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc
     9301 atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg
     9361 gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca
     9421 tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac
     9481 tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa
     9541 cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa
     9601 gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt
     9661 aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc
     9721 aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag
     9781 aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa
     9841 cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat acttttgata
     9901 ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc
     9961 agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag
    10021 tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt
    10081 ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca
    10141 tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta
    10201 ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat
    10261 taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg
    10321 ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa
    10381 attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa
    10441 ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc
    10501 gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc
    10561 gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg
    10621 cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa
    10681 gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca
    10741 gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact
    10801 gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca
    10861 gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga
    10921 atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg
    10981 tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta
    11041 actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg
    11101 aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg
    11161 caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat
    11221 acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta
    11281 gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc
    11341 tttataaaat taggaaaata attgttttct tttttcataa agactacctt tttgataaaa
    11401 tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg
    11461 acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc
    11521 tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc
    11581 aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg
    11641 agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta
    11701 taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat
    11761 cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat
    11821 cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt
    11881 aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt
    11941 ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc
    12001 attttatttg aactttcctg agatcactga catgcagaca attgttaaca attggaacaa
    12061 aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt
    12121 gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac
    12181 tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt
    12241 ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga
    12301 gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac
    12361 gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt
    12421 ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca
    12481 gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat
    12541 tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat
    12601 agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa
    12661 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
    12721 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
    12781 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
    12841 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
    12901 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
    12961 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
    13021 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
    13081 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
    13141 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
    13201 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
    13261 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
    13321 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
    13381 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
    13441 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
    13501 tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
    13561 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
    13621 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
    13681 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
    13741 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
    13801 aataatcatg acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca
    13861 atagcctaat tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt
    13921 ttcttataat aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa
    13981 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
    14041 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
    14101 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
    14161 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
    14221 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
    14281 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
    14341 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
    14401 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
    14461 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
    14521 agaaacaata aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca
    14581 gtttactacc ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa
    14641 gttttgataa atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg
    14701 aatctaccta gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa
    14761 ttgataaaaa ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg
    14821 tttatctttt tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata
    14881 agaaaagcag cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac
    14941 atttcaagca aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa
    15001 aatccgaatg tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca
    15061 agggctaata aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa
    15121 acgtcttttg caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg
    15181 ataccataaa atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa
    15241 caattaaaaa aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat
    15301 aaaaaaaata gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa
    15361 aaacgtcaga ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt
    15421 ctagatcttg gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt
    15481 ggaaagtacc accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt
    15541 caacactaaa cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt
    15601 ttataaattt tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata
    15661 ccattgaacc agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat
    15721 aaaaagcaac ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca
    15781 tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca
    15841 aaaatttaga ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga
    15901 tgtgatagaa tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat
    15961 aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta
    16021 tgaaaggaat ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc
    16081 gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac
    16141 gattaccttt atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc
    16201 ttgttttatt gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat
    16261 attttcgtaa acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta
    16321 aaggtctagt acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc
    16381 gggggatcaa gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat
    16441 ctacgttgat gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg
    16501 taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag
    16561 ggattttatg gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg
    16621 cgatcaaaga ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg
    16681 cccgatatta ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa
    16741 aattggcacc ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg
    16801 atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac
    16861 tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa
    16921 gaacgattga aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat
    16981 ccacgcaaag gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca
    17041 ttaattaagg aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt
    17101 caccaatgga ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg
    17161 gcagctgcag ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa
    17221 ttatcagaga aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc
    17281 gggatcggga tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa
    17341 ttagcgatga tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat
    17401 gcgatgacct tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct
    17461 gtgtcgattg ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa
    17521 tatgacgtgc tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa
    17581 cacttagaag gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct
    17641 gtaaaaaaat atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac
    17701 gaaaggcggt tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa
    17761 aggaatcagt agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa
    17821 agaaatcgga tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt
    17881 tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt
    17941 tatcacttgc tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat
    18001 tgtactgttt tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg
    18061 tggaagacac acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat
    18121 agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca
    18181 agcagcaatc tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt
    18241 ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag
    18301 tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat
    18361 cgcagctttg tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat
    18421 aacagcacag caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt
    18481 aacagaacat tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc
    18541 gggtgaagat atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt
    18601 gacaaacgtt ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag
    18661 taggaacacg acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg
    18721 tagaatcacc tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa
    18781 tggccagtgt tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa
    18841 acaattatga accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga
    18901 aagccgcagc aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag
    18961 aagcgattgc ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg
    19021 tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga
    19081 tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt
    19141 attcgatcag tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc
    19201 aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag
    19261 aagaatattg gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt
    19321 tctggggaat cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca
    19381 ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa
    19441 gaaaccctgc gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa
    19501 actttcgaac aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg
    19561 gtaaacaagg aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg
    19621 atacagcaaa agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta
    19681 gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc
    19741 gtccaacgag tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat
    19801 tgaaacttta tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt
    19861 tagatacaat gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa
    19921 aaatcaaatt caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag
    19981 agaaagaaaa tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg
    20041 aaccaaaaca acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg
    20101 cactagaaga aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc
    20161 aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa
    20221 ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag
    20281 aaatggaagg agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg
    20341 atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa
    20401 ttcaaatcaa agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga
    20461 taaaattagg gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata
    20521 cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca
    20581 ttgaacgaaa atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg
    20641 aattcacttc ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg
    20701 tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag
    20761 acatccaaaa ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca
    20821 gggcttgccg gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta
    20881 tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta
    20941 gtatgttcca attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa
    21001 gaaatgcgcc gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct
    21061 ggtggtgcct tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa
    21121 gagcatccat tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc
    21181 gtagtagcaa caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat
    21241 ggttcagatg gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc
    21301 gatatggata aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac
    21361 tgtccgatga cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg
    21421 gcagaactta aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta
    21481 atggcacagc gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa
    21541 gatcctaaaa ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca
    21601 gcttctttat acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt
    21661 gttttccgga tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac
    21721 cgaaacagct ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc
    21781 gaagatctgt tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg
    21841 agcggactac ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag
    21901 tttccacaag aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa
    21961 catttccaac cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga
    22021 gacaaaaaag caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata
    22081 aaaacagaat tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg
    22141 agacggatag tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg
    22201 aggaattgcg ccaattgtga acaaacttag aaatattggt taaattctga caatttattt
    22261 gtctttttat aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga
    22321 cagaagccac gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag
    22381 aaaaaacgaa agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat
    22441 ctggagacgc tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat
    22501 tagctgatca tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac
    22561 gttcgtttta tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg
    22621 ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag
    22681 cactttttga cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg
    22741 aagtccttta cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca
    22801 ttgattttga ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc
    22861 ctctaggttc ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat
    22921 tgatgacaaa atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa
    22981 tcacgcctac tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata
    23041 gtgatatcca tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg
    23101 cagatttgcg gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct
    23161 atgcccatgt aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt
    23221 aaaaataagg aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt
    23281 caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag
    23341 taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca
    23401 atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa
    23461 aatttgaagg caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag
    23521 ctcaagaatg gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga
    23581 atgataaaga aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta
    23641 tcttagctat cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact
    23701 atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag
    23761 atatctgtat ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat
    23821 atgtatgaat taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta
    23881 ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg
    23941 cagttagatg aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct
    24001 acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt
    24061 gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca
    24121 atggttcggg atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt
    24181 gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt
    24241 atcaaaaaag aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat
    24301 gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat
    24361 cgccgtacag tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt
    24421 gaagttgaag accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg
    24481 atcgacctaa atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta
    24541 acagtcaaag aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat
    24601 gctgatatcc atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat
    24661 gaaatcgata agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc
    24721 caaagagaca tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc
    24781 caaacagatc acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat
    24841 ctaattccag aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct
    24901 gaagattttg tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg
    24961 ttgatcggta cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct
    25021 caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc
    25081 attttggaaa aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa
    25141 ataacgatca cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat
    25201 ttaagtcgct atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa
    25261 cccgcaagat aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc
    25321 cttacgataa aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag
    25381 gaagtgacgg agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca
    25441 aaacaatgtt tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga
    25501 tgaaaactga agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa
    25561 gagagaaata tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt
    25621 tgggtacgat tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag
    25681 cagaatatgg agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg
    25741 aagcagatgt acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg
    25801 aattgaaggc tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg
    25861 cttctaatat tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa
    25921 aattggaatc agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa
    25981 aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg
    26041 actggggcag atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg
    26101 gaggaatggc aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac
    26161 acggtgcgga attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag
    26221 gtgatccagc gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa
    26281 aagatgatca gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc
    26341 gggatatgga ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga
    26401 gtacgccgga gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat
    26461 taggaggaga cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt
    26521 tcttttctat cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg
    26581 aagatttcta tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc
    26641 cattcatcga tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc
    26701 gtgaactttt tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca
    26761 ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag
    26821 ggatctggtc tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa
    26881 tcgctgacac ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag
    26941 gtgcccatga aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg
    27001 agaagttagc agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata
    27061 agggagtgtg acatcgattt gtcacactcc cgttttatct ctttggaatg aaaaagtatc
    27121 aggatttttt cttgctgcta ttcaaaccaa acgggattcg actttcagta tggtttctga
    27181 tacgcttgat attgtcttta tgccgataaa agataaagga agaaactaaa aaggcaatga
    27241 tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc tacataagga aggacgatcg
    27301 tagataaagt gatcaatact gcactgatca tactgctcaa actcaccata cttgtacaaa
    27361 ataaactaac gataaagatg atacaagaat aaacaaaaaa tacaggatta aatcctaaaa
    27421 gcattcctgc acttgtcgca acagcttttc ctcctttgaa cccagcaaaa atcggaaatg
    27481 tatggccaag aatggcgcat accccaaacc aaagagggtt aatcccagaa aggtggaaga
    27541 tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc cataagcagg acaacaatcc
    27601 cggctgtttt gcctaataca cgaaatgtat tggtcgttcc agtattaccg ctcccgtgtt
    27661 gtcggatatc ttttttgaaa aacaatttgc caatccagac accggaagga atagaaccta
    27721 aaagatatgc taaaataagt aataagatca atttcatttt ttaccctctt tcaagttgaa
    27781 aactatcctt tattttagca tgaataggta acaggaacaa ttgaaaaaat gaaagataca
    27841 agtataatag atggaataaa aagaaaagca aggggtggca gaaatgtctg taaaaaatcc
    27901 aacacaagaa acgattttca actatttgga agaagcaaaa aatattgccg tcgtaggctt
    27961 gagcaataaa ccagaacgaa caagttacat ggttgcacag gtattgcaag acaatggata
    28021 taaaatcata ccagtaaatc cattattggc tggtgaagaa attctaggcg agaaagtcta
    28081 tgcgaatttg acagaaatcg atgatccaat cgatatcgta gatgtattta gacggagtga
    28141 atttttacct gaaatagcag gtgatttttt gaaaactgat gctaaagtgt tctgggcaca
    28201 attagggatt gaaaacaaag aagcagctga gatgctaaga aaagcaggac gtacagatat
    28261 cgtaatggat cgttgtatta agattgaact tggtaagttg aaagaaaacc aaaaataaaa
    28321 atgaccacaa agaagagatg atcgatagag gggaatgtcg aaaaagcggt taggagataa
    28381 ttaaaattga tttctcgtct accctctttt ttttcacgac cttttctagt atgataatcg
    28441 tggtgcggtt ttgccgcaaa gtattattga aggagcgttt gccttggcaa aaaaagtaaa
    28501 caacgaatac aatgactcgt ccatccaagt attggaagga ttagaggcag ttagaaaaag
    28561 accagggatg tacattggat ccactgacag ccgaggcttg catcatttag tctatgaaat
    28621 cgtcgataat gcagttgacg aagcattatc aggttatggc agtgagattg atgtgaccat
    28681 ccatgaagac aatagtataa ctgttgcaga tagcggacgt gggatgcctg taggaatgca
    28741 tgcttccgga ataccaacag ttgaagtgat ttttactgtg ctgcatgctg gcggaaagtt
    28801 tggtcaagga gggtacaaaa cttctggtgg actgcacggt gtaggagcca gtgtggtaaa
    28861 tgccttatcg aaatggctaa ttgtaacgat cgttagagat ggcgtagagt atcagcagaa
    28921 atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag ataggaaaaa caaaaaaagc
    28981 aaacgggacg actgtccatt ttttaccaga tgatacaata ttcagtacaa cgaaattttc
    29041 atatgaaatt ttagccgaac ggttaagaga atctgccttt cttctaaaag gcgttaaaat
    29101 ctctctttct gatcttcgtg gagaagaacc agtcaaggaa atttttcatt acgaagaagg
    29161 aatcaaagaa tttgttgatt atctgaatga agaaaaagat acattgacac cagttgttta
    29221 tttttcagga gaaaaagaag gtatcgaagt agaggtcgct tatcaataca atgacggtta
    29281 ttcagaaaat gttttatctt ttgtcaataa cgttcggaca aaagatgggg gaacccatga
    29341 agctggtatg aaagcggcga tgacgaaatc ttataatgaa tatgcgagaa aagttggttt
    29401 gctgaaagaa cgggacaaga atctggaagg tagtgatttc agagaagggt tagctgctgt
    29461 actttctatc cgtgtacctg aaaatcttct gcaatttgaa gggcaaacaa aagaaaaact
    29521 tggtacgcca gttgctcgta ccgtagtaga taacgtcatc agtgaacaaa tgggctttta
    29581 tttgcaggaa aatagcgaaa tgagccagat gttggtccgt aaagcaatca aagccagaga
    29641 agcgagagaa gcagcacgta aagccagaga agaaagccga aatgggaaga agcgtaaaaa
    29701 aggcgaatct cttctttcag gaaaattgac acccgcacaa tcccgaaacc ctaagaaaaa
    29761 tgaattgtat ttagtcgaag gggattctgc tggcggatct gctaaacaag gaagagacag
    29821 aaagttccaa gcaattctac ctttacgagg aaaagtcatc aataccgaaa aagctaaaat
    29881 gcaagatatc ttgaaaaatg aagaaatcaa tacgatgatc tatacgattg gtgcaggagt
    29941 cggtcctgag ttttctatcg aagactgtaa ttacgacaaa gtaatcatca tgaccgatgc
    30001 ggatacagac ggcgcccata ttcaagtatt actgcttact ttcttttatc gttatatgaa
    30061 accgctgatt gaagctggga aagtatatat cgcgttaccg cctttatata aagtatccaa
    30121 aggacaagga aagaagcaag tcattgaata tgcgtggaca gatgacgaat tggcagcaat
    30181 gattaaaaaa gtcggcaaag gatatatgct gcagcgatat aaaggacttg gagagatgaa
    30241 tgcggaacaa ttgtgggaaa caacgatgga tccgacttcc cgtacgttga tccgcgtccg
    30301 tatcgatgat gctgcgcaag ctgaacgtcg agtaaccaca ctgatgggtg acaaagtaga
    30361 acctcgaaga aaatggatcg aaaatcacgt gcaatttacg ctcgaagaag atggcagtat
    30421 cttagataaa aaagaggata ctgaaatctc tccttcagtt tctaatgatt tattagatga
    30481 agaacgagca gataaaaatg aaaacaatca gttatttgaa gtagaatagg aggtcacctg
    30541 atggaaaatc gacaagaagt acaagaatta actttagaag aagtgatggg tgaccgcttc
    30601 ggacgttatt ccaaatatat tatccaagaa cgtgcactgc ctgatatccg tgatggattg
    30661 aagccagtgc aacgccggat attatttgcc atgaacaaag atggcaatac gtatgacaaa
    30721 ggattccgta aatcagcaaa atctgtcgga aatatcatgg ggaattatca tcctcatggt
    30781 gacatcagta tttatgaagc gatggtccgc atgagccaag actggaagtt gcgggaagta
    30841 ctgatcgaaa tgcacgggaa taacgggagt atggacggtg atccacctgc tgcgatgcgt
    30901 tatactgaag ccagattatc caagttaagt ggagagatgc tagcagatat cgagaaagaa
    30961 acagtcgatc tcgtgtggaa ttttgatgat acagaaaaag aaccaacagt ccttccagct
    31021 cgatacccaa accttctcgt caatggatcg acaggtatat ctgctggata tgcaacagaa
    31081 atcccgactc ataacttagc tgaagtgatc gatggcacaa tctatatgat cgatcatcca
    31141 caagcgagcc ttgaaaaatt gatggaatat attcctggac ctgactttcc aacaggaggg
    31201 atcttgcaag gaaaagatga aatcaaaaaa gcttatgaaa caggacgagg aaaagttatt
    31261 cttcgttcga aaacaaagat agaggcaatg aagggtaata aacagcaaat cgtgatcaca
    31321 gaaatcccat atgaagtaaa taaagcgacc ttggtgaaaa aaatggatga gatccgtttg
    31381 aataagaaaa tcgatggaat agctgaagta cgagatgaaa gtgatagaac aggtctgcaa
    31441 attgtcgtcg aattgaaaaa agacgccaat gctcaaggaa ttttgaatta tttgttcaaa
    31501 aacactgagc tacagatcaa ttacaatttc aatatggtcg ctatcgacca tatgactccg
    31561 catcaagttg gactgaaaga cattttaaga agttatatcg agcatcgaaa gcaagtcata
    31621 acaaaacgta gtcaattcga tttagccaaa gctcaaaaaa gacagcatat cgtagaagga
    31681 ttgatgaaag ctttatcgat tctggatgaa gtaatcgcaa cgattcgtga aagtaaagac
    31741 aaaaaagacg ccaaaaagaa tttagtcgac gtctttcaat ttacagaaga acaagcagaa
    31801 gcaattgtca cattgcaatt ataccgtttg acgaacacgg atatcacaga gcttcaaaag
    31861 gaatccgaaa gcttgattgc gcaaatcaca gagttgaata aaatcctttc aaatgacaaa
    31921 gagcttttct ctgttatgaa aaaagaattg cgagaagtga aaaaaaatta ttcttccgct
    31981 cgtctaacga caatcgaaga cgaaatcgaa gaaatcaaaa ttgatacgca agtcttagtg
    32041 gcacaagaag acgtaattgt cagtgtgacg cgagaaggct atgtgaagcg aacaagcctt
    32101 cgttcttata gtgcttccaa accagaagaa atcgggatgc gagaaggcga ctacttgcta
    32161 tactcaggtg aattgagtac attggaccat gttcttctga tcacaaataa agcaaatgtg
    32221 atctaccgcc ctgtccatga actacccgat ttgaaatgga aagatgctgg tgagcacatt
    32281 tctcaaacaa tctccaatct gtctgtagac gaatcgattt tagctgtttt cccatatcaa
    32341 aaaattcaag cagagaaaac atttgtattc atcagcaaga atggattgat caagcaaaca
    32401 cgaatgacag agttcgaacc atggcgcacc tataagagcc gtccgctcag ctgtatgaag
    32461 ctaaaggcgg aagaagatga actgattgct gtgtatctga aagaagatca gccaggctta
    32521 gatgttttct tagtaacgca tcaaggaatg ggtttgcgct atcctttaac agatgttccc
    32581 gttgttggag caaaggcagc tggagtgaag tctatgaatt tgaaagatgg agataggatt
    32641 atcaacgggc tgcttgtgtt agcagaaggt gatacgccga tcgtaatcgt tactcagcga
    32701 ggagctgtta aacgcatgct tgctcaagaa atcagccaaa cttcacgtgc gaagcgggga
    32761 gtgacggtca tgcgtgagct gaaaaaaaat ccgcaccgta ttatctatat gtcggaagga
    32821 agatcaaaag aaataacctt gatgaatcaa aaagggcagc agttaacgat tgacccaact
    32881 gatttcccta taggtgatcg aacatcgaat ggatcatttg cgatggatga gaaaaaaggt
    32941 ggagaagtca tcgaagttat cgatgctcct gagattttca tcgagtcgta actgttgtag
    33001 tattttaatg aagagacgcc atctgtatta atacaggtgg cgtctctttt tcattcattt
    33061 ttaataaatc cttatacgtt tgcaaaaaaa gatgaaagtg ttcacaatgc ttaaatgttg
    33121 gtatgtcaac gttgatttaa atatatgtaa ataaaacata aaaattgtta aataaaaaac
    33181 aagaaaaccc ttgcaaagat agggtgctgt gttatactaa tcacgtaaaa actgttacat
    33241 aaaacagaaa tttaggaggt ccatacaatg gagcaatgga atggatttaa aggtaccaaa
    33301 tggaaagaag gcgtagacac tagagacttt attcaaaata actacactga atatcgcggt
    33361 gatgacagct ttttggaagg cccaacagaa gcaacagaaa aattatggga tgaattgcaa
    33421 aagttaaatg atatccaatt cgagcgtaac ggtgtctatg atatggacac agaaatcgta
    33481 tcaactatta cttcacacga tgcaggctat ttggataaag atttggaaaa aatcgtcggt
    33541 ctacagacag acaaaccttt gaaacaagca ttcatgccgt tcggcggaat caatatggct
    33601 aacaatgcgc taaaaagcaa tggctataca ccaaacgatg aattgacaca tattttcact
    33661 gactggcgca aaacacataa tcaaggtgta tttgacgctt atactgctga aatgcgtgct
    33721 gctcgtaaaa acaaaatcat tacagggctt ccagacgctt acggtcgcgg acgtatcatc
    33781 ggtgactatc gtcgtgtggc attgtatgga atcgactatt tgatggaaca aaagaaaaaa
    33841 gatcatgata tgactggtta tcatacaatg agtgaagaag tgattcgttt acgtgaagaa
    33901 atcactgaac aatatcgtgc attagcgcaa atgaaagaaa tggctgcgaa atatggctat
    33961 gatatttcta aaccagctgc aaatgcaaaa gaagctgtcc aatggctata cttcggatat
    34021 ttagcagcaa tcaaatcaca aaatggtgct gcgatgtcaa tcggacgtat ctcagcattc
    34081 cttgatatct atatccaacg tgatttggat aatggagtaa tcacagaaaa agaagcacaa
    34141 gaattgatcg accacttagt aatgaaatta cgtatggtca aattcgcgcg tacacctgaa
    34201 tataaccaat tattctcagg attccctatc tgggctactt tatccattgc aggtatggga
    34261 ttagatggtc gttcattagt tacaaagaat gatttccgtc tgcttcatac attaacaaat
    34321 atgggacctt caccagaacc aaacttgact gttttgtatt ctgaacacct tccagaagga
    34381 ttcagaacat atgctagcag aatcgctatc gaaagttctt cgattcaatt cgaaaatgat
    34441 aatcagcttc gtgaacaatg gggttctgat gattgtgcga ttgcttgctg tgtatcagga
    34501 acagttgtcg gaaaagacat gcaattcttt ggcgctcgtg ctaacttggc taaaacacta
    34561 ttatatgcca tcaatggcgg agtcgatgaa gtgactggcg cccaagttgg accagaatat
    34621 cgtccaatca catcagaaat actagacttt gaagaagtga aacataactt catgaatatg
    34681 atggattggt tagcagaact atatgtcaat acgttgaaca tcattcatta tatgcatgat
    34741 aaatattctt atgaagcagc tcagctggct ttgatggatt ctgacttgca acgcacattt
    34801 gctacaggta ttgctgggat ctctcatgcg gcagacagct tgtcagcgat caaatatgca
    34861 aaagtaaaag caatccgtaa tgaaaaagga atagcagttg attttgaagt ggatggcgac
    34921 ttccctaaat acggaaatga tgatgaccgt gcagatgaat tagtttcatg gatcttggaa
    34981 tacttcatgg gacaaatcaa acgtcataaa acttaccgca actcaaaacc aacaacttca
    35041 ttattgacga ttacttcaaa tgttgtttac ggtaaaaata caggaaatac tcctgatggc
    35101 cgtgaagctg gaaaaccact tgcgccagga gcaaatccaa gttacaatgc ggaacaaagt
    35161 gggttattag ctagcctgaa ctcaacagct aaaatgaatt acggctgtgc attggatgga
    35221 atttcaaata cccaaacaat caatccttca ggtcttggaa aagaacaaga tacaagaatc
    35281 gaaaacttgc gtaatgtctt agatggatac ttctcaaaag gagcttatca tttgaatgtc
    35341 aatgtattca gtaatgattt attgcgtgat gcaatggaaa atccaatgaa atatccgaac
    35401 ttgacgatcc gggtatctgg ttatgcagtt aaattccgtg acttgacaag agaacaacaa
    35461 ttagatgtct tgatgagaac ttcacacgac agaatgtaaa aagttcgtga aaaaagtttt
    35521 tcgacttagc gtcgaaagga cgttgaatag ccatcgcatc aaaatcgaat aatcagtatg
    35581 cttatttgaa agaaacgatt tacaggggca ggacttgtat gttccgtccc tgtaaatttt
    35641 acacataacg aagcggcaat tgcgaaaaat tttgttcaga aagcttcgct tcgacagcta
    35701 acttgtactt aaaatgaaat caaagaaaag atgtgaactt aaatggaaga aaaaacaatc
    35761 ggctatgttc attctatcga aactttcggt tcagtagacg gacctggact gcgctttgtc
    35821 gtgtttatgc aaggatgccg catgcgttgc caattttgtc acaatccaga tacttggaat
    35881 attggcggag ggaaagaata cacagcagat gaacttttag ataaagcaga acgcttcaga
    35941 ccttactggg gagataaagg tggtattact gttagtggtg gagaacctct attgcagatc
    36001 gattttttga tcgaactatt taaaaaagca aaagagcgga agatgcatac tacattggat
    36061 acatgcggaa aaccttttac gtatgaagaa cctttctttt cacgttttca agaattgatg
    36121 aagtatacag atttactgtt atttgacatc aaacacatcg ataatgaaga gcataaaaaa
    36181 ctaacgcatt gggacaatga aaatatttta gaaatggctc aatatttgtc aaaaatcaac
    36241 aaaccggttt ggattcgcca tgtactagtt cctgagcgaa gtgattatga tgaatatttg
    36301 atccgactag acaactttat tcaaaccttg tcaaatgtag acagagtaga gattcttcct
    36361 tatcatacga tgggaaaata caagtgggaa acacttggtt tgaaatatcc attagaaggg
    36421 atcgaaccac ctacaaaaga acgtgtcgaa aatgctaaac gattgcttca tacagaagag
    36481 tatacaaaat atttaacgcg ataggtcgca tcggaaagaa gataagtatt gcttctttcc
    36541 gtttttttgc tattattaaa taggagaaat cacaaaggag cgaatgacat gtcaaaaatt
    36601 ttagtttttg gtcaccaaaa tccagataca gatgcaatcg gggcagccat tgcgttttca
    36661 cacttgcaaa atgaattagg taaagaaaca gaagcagttg ctctaggtac acctaatgaa
    36721 gaaacacaat acgcattaga ccactttcag ctatctgcac cacgagtagt agagtcagct
    36781 aaagcagaaa atgcagatgt catgcttgtg gatcataatg aattccaaca aagtatttca
    36841 gatatcagcg aagtaaatat tttagctgta gtagaccacc atcgtattgc aaactttgaa
    36901 acagctaatc cattgtacta tcgtgcagaa ccagttggat gtacaagtac gattatttta
    36961 aaactatata aagaaaacca agtaacaatt cctaaagaaa tcgcaggaat catgctctca
    37021 gctatcattt cagatacatt gttgttcaaa tcaccaacat gtacgcctga agatgaagca
    37081 gctgcaaaag aacttgcagc aatcgctgaa gtcgatctaa acacttatgg acttgaaatg
    37141 ttgaaagcag gaacaaatct aagtgataaa tcagctgcgg ttcttcttga tttggatgca
    37201 aaaagtttcc caatgggcag ttccaacgtt cgtatcgctc aagtcaatac agtggacctt
    37261 aatgaagtga tggatcgaca agcagagcta gaagcagcaa tggaagatga aatgaaagca
    37321 aatggctacg atctgttcgt attagttgtc acaaacatct tagacagtga ttcagaatta
    37381 cttgttgctg gtaaaggaaa agaaaaagta gaagaagctt tccaaacaac tttgaccaac
    37441 aaccgtgctt tgcttaaagg tgtcgtttca cgtaaaaaac aagtcgtacc tcaattgacg
    37501 gctgtattta gctaatcaaa tggtagtatg aagcattcaa aatgaatgga taagcgattg
    37561 atataaaaag aaacatagaa catactaaag gccgaagaaa tggaggagaa taacagtcaa
    37621 ttatgactct tgttcttcct cctttttacg taaaagcctt ttaagtaaaa taaaacggat
    37681 agaaaagaga acatatatga actattattc aaaaaataag gaaaactttt atcaaaaact
    37741 gaccgatgat ccgctttttt cttcattgac cgattatcta tatgaacatc gagaacaaga
    37801 aacgattcta agagaattga aaaaagaatt ttcgcaaaat aaattttccc attttcttga
    37861 tttattgatc gatgcaggat taatcaagag ggaagaacga agatatcatt tgaactttcc
    37921 aatatttgat cctaaagatt acttgcaaca agccacttcg gctgctgaaa ccatagctga
    37981 acaattgaag agactctctg ttgatgaaca aaaattagcg atgggagaag ttatttgggc
    38041 gtattgtttc gaagatgaac ggaaagaagc gtatttttat ggagtaagaa acagccgaga
    38101 gacagaattg ctgaggacaa ctgcaggaaa tgagaagtac cgttttatca cactttcctc
    38161 tatagaacac ttcccgctta ctttggctaa ttactttttt gtacagaaga atcagcttcc
    38221 tgtaacgaag gcattcaaag aattagccga acttattgga gacgtaaatg aatcgtactt
    38281 ttttgatcaa atagaagtta tcgttgacag gatacgtaag aataaatata aaaatcgccg
    38341 gcctagtatt ttccatcagt ctctacttgt gacgaatact ataaaggaag aagaatcatt
    38401 cacattagaa ttacctattg tcgaaaaaaa caattttgag attgaattac caacattgga
    38461 tccttcattg acaatggagg aaatggcctt tcttaaacga caaatctttt ctgagctgag
    38521 caagaaattt ataccgcatg cattttctta tatcaaagaa tatggaacag ttttagtgag
    38581 taaaacctaa gagagcagta agcaaaaatt atactgactc ttgcaggagg agcctttctc
    38641 ttcctttatg gttaaaagaa ttatttgagt attttgccta tctaccacct attagattca
    38701 ctacatggtc tggaaacaaa ggaatgcaaa aaaatggtga gaaatgtgga atgacaaagg
    38761 aagtagtgat aagaaaagtt agatttttaa ataatcaata ttatgattca gtcaagtatg
    38821 gcattttatg ggaagaacta gctgattaga ttaggaagtc catttgataa tccagaatca
    38881 ctaagaaacg aatggcgagt cattaataat ttatccaatc ttgggttatt cttttagatt
    38941 gaagtgttta aaactaaaaa agcatttatg aagtcgtaat agtcaggctt caaaatgctt
    39001 tttattttgt ttgttttttc tttgccacat aaatacacct agactgacga tgaagagcat
    39061 atccaacaga tttcctgcga cttggatcgt cgaagggttg gtcagtggca ttaaagcaag
    39121 gacaaaaaag gaaaacaagc tgtaaataaa acaggtgaca ggaactaatc gaaagaagag
    39181 cggacatttc aattggctaa aaatgagtga aaaaataaaa taagtaaaaa tggataaaat
    39241 cacgactaaa ccattcatga taaagaagaa aagcgtattg tgattaaacg aataaaaaag
    39301 aattgctaaa aaagaaaacg ttttaaatgc aatcaatacg cttgaagctt cgtttaacgt
    39361 accaggatgt cgatgaaaaa aataggcacc gaaataaaca gaaaacagta agccgcaaag
    39421 agaagacaaa atcaaaaaaa acggattttc ttcggcttct gtgatatcag aatgatccaa
    39481 aagcaaccag cctaatgcac tagaaggcgc atgtgaggtc ttaaggtatg aataacatag
    39541 aaaaaataag atcgtcaaag ttgcaaaaaa agagcctaaa tgtaaaaaat tagtggttct
    39601 acgttgaatt tccataatat ctccaaatta aattgataag tatataaagt ataataacaa
    39661 attgatgagt atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt
    39721 taaaatttaa tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt
    39781 tctcactttt ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa
    39841 agcacgctct tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga
    39901 aagtatttcg agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc
    39961 ataaccgtgt tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa
    40021 aatcgttgtg accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact
    40081 gatacctact gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc
    40141 taaaacaagg gataagccga cgatataaat cgtgatctcg accatactga caagtggagc
    40201 aatcagtcga tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc
    40261 aaaattaatg aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt
    40321 catttatata acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac
    40381 atacaaaaaa atgataaaat gattataata gaattataaa gataggttag gtgattaaaa
    40441 tgaaaacggt caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat
    40501 acttaggtgt ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt
    40561 cgctgacaga tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata
    40621 ttttcggtag cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat
    40681 taggagtggt gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta
    40741 tattatttgg tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat
    40801 caggaaattt aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg
    40861 tccatcatat tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg
    40921 ttgtcatcta ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa
    40981 aaattgcgca gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca
    41041 aacagccggt ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt
    41101 ttgaatttga cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg
    41161 cagttggcgg ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc
    41221 atatagtagc gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa
    41281 tcgtaaggcg cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt
    41341 catgggttaa gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt
    41401 tcttttggca tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag
    41461 aaaaaaatcg gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc
    41521 gacatatggt acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga
    41581 taaaaaagta taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt
    41641 tttattttgg gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg
    41701 acaacatgtt agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa
    41761 tgtttcaaaa atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt
    41821 gaaagagtta gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa
    41881 agcacttgtc acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac
    41941 aactgagccc tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca
    42001 ttattctctc caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat
    42061 catcacaggt gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt
    42121 cttgtttggg gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac
    42181 agcaaaagca acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat
    42241 cgaagtggac gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga
    42301 agttgatctt cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata
    42361 tatgacagaa aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg
    42421 gctagcacta ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt
    42481 tggtgtgatt ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac
    42541 gatcaagcaa gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat
    42601 agaagaaaaa ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga
    42661 gacaacgaat taagtcttat tactatatat atatttatga aattcatcgc tattttctta
    42721 aaataatcag cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc
    42781 atttttgaga aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc
    42841 gtacgatgat gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg
    42901 acaagaagat cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca
    42961 tttcacgaac acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa
    43021 gtcagctccg cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg
    43081 ccattaccaa tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg
    43141 ttctgtcaga caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt
    43201 tgacacgaag gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat
    43261 cccagaccct agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac
    43321 ggatgactct tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg
    43381 tgagccgtct gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga
    43441 agaaaaaaaa tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga
    43501 tgatcaagga aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat
    43561 attcggctac agttggaaag gaaacgctgc ggcgtttcct tattttatac tctatccgaa
    43621 agtttatatc gtatcctcaa atttttttga g
//
LOCUS       DAZUJO010000015        43375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000015 DAZUJO010000000
VERSION     DAZUJO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.015"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..2714
                     /gene="adhE"
                     /locus_tag="ACG8OL_000910"
                     /gene_synonym="adhC"
     CDS             117..2714
                     /gene="adhE"
                     /locus_tag="ACG8OL_000910"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS4816915.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2943..4049
                     /locus_tag="ACG8OL_000911"
     CDS             2943..4049
                     /locus_tag="ACG8OL_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4816916.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4066..5094
                     /locus_tag="ACG8OL_000912"
     CDS             4066..5094
                     /locus_tag="ACG8OL_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS4816917.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5100..5579
                     /locus_tag="ACG8OL_000913"
     CDS             5100..5579
                     /locus_tag="ACG8OL_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS4816918.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5595..6890
                     /locus_tag="ACG8OL_000914"
     CDS             5595..6890
                     /locus_tag="ACG8OL_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS4816919.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6901..7725
                     /locus_tag="ACG8OL_000915"
     CDS             6901..7725
                     /locus_tag="ACG8OL_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4816920.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7890..8789
                     /locus_tag="ACG8OL_000916"
     CDS             7890..8789
                     /locus_tag="ACG8OL_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4816921.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8915..9784)
                     /locus_tag="ACG8OL_000917"
     CDS             complement(8915..9784)
                     /locus_tag="ACG8OL_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4816922.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9937..11397
                     /gene="rhaB"
                     /locus_tag="ACG8OL_000918"
     CDS             9937..11397
                     /gene="rhaB"
                     /locus_tag="ACG8OL_000918"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS4816923.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11394..12650
                     /gene="rhaA"
                     /locus_tag="ACG8OL_000919"
     CDS             11394..12650
                     /gene="rhaA"
                     /locus_tag="ACG8OL_000919"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS4816924.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12664..13509
                     /gene="rhaD"
                     /locus_tag="ACG8OL_000920"
     CDS             12664..13509
                     /gene="rhaD"
                     /locus_tag="ACG8OL_000920"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS4816925.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13511..13822
                     /gene="rhaM"
                     /locus_tag="ACG8OL_000921"
     CDS             13511..13822
                     /gene="rhaM"
                     /locus_tag="ACG8OL_000921"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS4816926.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13904..15055
                     /gene="fucO"
                     /locus_tag="ACG8OL_000922"
     CDS             13904..15055
                     /gene="fucO"
                     /locus_tag="ACG8OL_000922"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS4816927.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15182..15940
                     /locus_tag="ACG8OL_000923"
     CDS             15182..15940
                     /locus_tag="ACG8OL_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS4816928.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16040..16684
                     /locus_tag="ACG8OL_000924"
     CDS             16040..16684
                     /locus_tag="ACG8OL_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS4816929.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16691..17698
                     /locus_tag="ACG8OL_000925"
     CDS             16691..17698
                     /locus_tag="ACG8OL_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS4816930.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17714..18556
                     /gene="kduI"
                     /locus_tag="ACG8OL_000926"
     CDS             17714..18556
                     /gene="kduI"
                     /locus_tag="ACG8OL_000926"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS4816931.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18576..19379
                     /locus_tag="ACG8OL_000927"
     CDS             18576..19379
                     /locus_tag="ACG8OL_000927"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS4816932.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19628..20692)
                     /gene="fni"
                     /locus_tag="ACG8OL_000928"
     CDS             complement(19628..20692)
                     /gene="fni"
                     /locus_tag="ACG8OL_000928"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS4816933.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20689..21774)
                     /locus_tag="ACG8OL_000929"
     CDS             complement(20689..21774)
                     /locus_tag="ACG8OL_000929"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS4816934.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21787..22764)
                     /gene="mvaD"
                     /locus_tag="ACG8OL_000930"
     CDS             complement(21787..22764)
                     /gene="mvaD"
                     /locus_tag="ACG8OL_000930"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS4816935.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22757..23701)
                     /gene="mvk"
                     /locus_tag="ACG8OL_000931"
     CDS             complement(22757..23701)
                     /gene="mvk"
                     /locus_tag="ACG8OL_000931"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS4816936.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24017..24676
                     /locus_tag="ACG8OL_000932"
     CDS             24017..24676
                     /locus_tag="ACG8OL_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4816937.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24761..25666
                     /locus_tag="ACG8OL_000933"
     CDS             24761..25666
                     /locus_tag="ACG8OL_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4816938.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25668..26300)
                     /locus_tag="ACG8OL_000934"
     CDS             complement(25668..26300)
                     /locus_tag="ACG8OL_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4816939.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26451..26576
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26620..27144
                     /gene="infC"
                     /locus_tag="ACG8OL_000935"
     CDS             26620..27144
                     /gene="infC"
                     /locus_tag="ACG8OL_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS4816940.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27216..27416
                     /gene="rpmI"
                     /locus_tag="ACG8OL_000936"
     CDS             27216..27416
                     /gene="rpmI"
                     /locus_tag="ACG8OL_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS4816941.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27469..27828
                     /gene="rplT"
                     /locus_tag="ACG8OL_000937"
     CDS             27469..27828
                     /gene="rplT"
                     /locus_tag="ACG8OL_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS4816942.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28080..29417
                     /locus_tag="ACG8OL_000938"
     CDS             28080..29417
                     /locus_tag="ACG8OL_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS4816943.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29465..31594
                     /locus_tag="ACG8OL_000939"
     CDS             29465..31594
                     /locus_tag="ACG8OL_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS4816944.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31569..32258
                     /locus_tag="ACG8OL_000940"
     CDS             31569..32258
                     /locus_tag="ACG8OL_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816945.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32666..33279)
                     /locus_tag="ACG8OL_000941"
                     /pseudo
     CDS             complement(32666..33279)
                     /locus_tag="ACG8OL_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(33415..33609)
                     /locus_tag="ACG8OL_000942"
     CDS             complement(33415..33609)
                     /locus_tag="ACG8OL_000942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816946.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33659..34099)
                     /locus_tag="ACG8OL_000943"
     CDS             complement(33659..34099)
                     /locus_tag="ACG8OL_000943"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS4816947.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34103..34948)
                     /locus_tag="ACG8OL_000944"
     CDS             complement(34103..34948)
                     /locus_tag="ACG8OL_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS4816948.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35097..36515
                     /gene="pepV"
                     /locus_tag="ACG8OL_000945"
     CDS             35097..36515
                     /gene="pepV"
                     /locus_tag="ACG8OL_000945"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS4816949.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36572..37519)
                     /locus_tag="ACG8OL_000946"
     CDS             complement(36572..37519)
                     /locus_tag="ACG8OL_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4816950.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37742..38092)
                     /locus_tag="ACG8OL_000947"
     CDS             complement(37742..38092)
                     /locus_tag="ACG8OL_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS4816951.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38292..39371
                     /gene="pepA"
                     /locus_tag="ACG8OL_000948"
     CDS             38292..39371
                     /gene="pepA"
                     /locus_tag="ACG8OL_000948"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS4816952.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39515..39835
                     /locus_tag="ACG8OL_000949"
     CDS             39515..39835
                     /locus_tag="ACG8OL_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS4816953.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39857..40321
                     /locus_tag="ACG8OL_000950"
     CDS             39857..40321
                     /locus_tag="ACG8OL_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4816954.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40526..41131
                     /gene="ytpR"
                     /locus_tag="ACG8OL_000951"
     CDS             40526..41131
                     /gene="ytpR"
                     /locus_tag="ACG8OL_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS4816955.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41170..42459)
                     /locus_tag="ACG8OL_000952"
     CDS             complement(41170..42459)
                     /locus_tag="ACG8OL_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS4816956.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42887..43366
                     /gene="rlmH"
                     /locus_tag="ACG8OL_000953"
     CDS             42887..43366
                     /gene="rlmH"
                     /locus_tag="ACG8OL_000953"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS4816957.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT        13719 a         7925 c         9299 g        12432 t
ORIGIN      
        1 atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt acatagaaaa ataaatattg
       61 tatgatatac atgtgaatta tttaacaaat aaccatgacc cattaggagg ataatgatgg
      121 ctaaagtaat agaaaaagaa aaaacgaaga caattgacgt acaagcaatg attgatgatt
      181 tggcagaaaa agcaaatgca gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag
      241 accatatcgt gcatgaaatg gcaatggcag ctttggatca acatatgcca ttagcgaaga
      301 tggctgtaga ggaaacaggc cgcggtgttt acgaagataa agcaatcaaa aatatgtatg
      361 cgtccgaata tatttggcat aatatcaaac atgataaaac agttggtgtg atcaatgaag
      421 atgtccaaaa agggttgatt gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc
      481 caacaacgaa tccgacttcc acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa
      541 atccaatcgt ttttgctttc catccaagtg cccaaaaatc ttctgcagaa gcagcacgag
      601 tggtacgtga tgctgctatc gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac
      661 atccttcgat cgaagcgact tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag
      721 cgacaggtgg agctggaatg gtcaagtctg cttactctac aggaaaacca gctttaggtg
      781 taggtccagg taacgtacca gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga
      841 acgatttgat cgtttcgaaa acatttgata atggaatgat ttgtgcttct gaacaagctg
      901 tgatcgtgga taaagaaatc tatgcagcag taaaagcaga attccaagca caccaagttt
      961 atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat
     1021 atgcggtcaa tccatcaatc gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa
     1081 tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt
     1141 atccgctatc aagagaaaaa ctttctccag ttcttgccat gatcaaagct aacaacacgg
     1201 accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg aggattagga catacagcag
     1261 ttatccattc agaaaacgaa gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc
     1321 gtattttagt gaatacacca tctgcagaag gcggcatcgg tgatatctat aacgaaatga
     1381 ttccgtcatt gactttggga tgcggttctt atggtaagaa ctctgtttct cgtaacgtct
     1441 ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg gagaaataac atgcaatggt
     1501 tcaaattacc gccaaaaatc ttctttgaaa agaactctct attgtactta gagaaaatgg
     1561 aaaacgttga acgtgtcatg atcgtttgtg accctggtat ggtccaattc ggttactgcg
     1621 atacagttcg tgaagtattg gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag
     1681 atgttgaacc gaacccatcg acaaataccg tatacgcagg aacgaaactg atggcagact
     1741 tcaagccaga tacagtaatc gctcttggag gcggttctgc aatggacgca gctaagggaa
     1801 tgtggatgtt ctatgaacac ccagacacat ctttctttgg tgcaaaacaa aaattcttgg
     1861 atatccgtaa acggacatat aagatcgaca aaccagaaaa aacacaattc gtatgtatcc
     1921 cgacaacatc agggactggt tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa
     1981 cacatgtgaa atatccgtta gcagattatg cattgacgcc agatgtcgca atcgtcgatc
     2041 ctcaattcgt catgtcagtt ccagcatctg tcacagcaga taccgggatg gatgtgttaa
     2101 cacatgcaat cgaatcctat gtttcagtca tggcttcaga ttatacacgt ggattgagcc
     2161 tacaagcaat caagttagtc tttgactatt tagaaaaatc agtaaaaaca cctgatatgg
     2221 aatcacgtga aaaaatgcac aacgcttcta caatggctgg tatggcgttt gctaacgcat
     2281 tcctaggtat ttgtcactca gtcgcacata aaatcggcgg ggaatacggg atccctcatg
     2341 gacggacaaa tgcgattctt ttaccgcata ttatccgcta taatgcaaaa gatccacaaa
     2401 aacatgcgat gttccctaaa tatgactatt tccgtgcaga tacagattac gcagatatcg
     2461 caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg
     2521 ctgtttatga attaggaaaa tctgtcggca tcaacatgag tctaaaagct caaggagtca
     2581 cacaagaaac actagataca accgtagacc gtatggctga attagcttac gaagaccaat
     2641 gtacgacagc taatccgaaa gaaccattga tctctgaatt gaaacagatc atcattgatg
     2701 cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa
     2761 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
     2821 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
     2881 tggacggtat tactgctata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
     2941 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
     3001 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttggactttg
     3061 atggactgaa agtagatgac aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc
     3121 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
     3181 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
     3241 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
     3301 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
     3361 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
     3421 tgacagtgct tcctttggca aaaatcggga aactgttgaa tcgcccagaa tatttagaag
     3481 aagctcgtta ccagtttatg atccatatca aatacttgat ggataaacga acaggattgt
     3541 ggtatcacgg ctggacgttt gaaggaaatc ataactatgc cgaagcattt tgggctcgtg
     3601 gaaactgctg gttgacgatt gctattccag agatcattga aatcttagaa ttatcgaaag
     3661 aagatgcatt gcgcaaatta ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa
     3721 cttatcagtc tgaaagcggt ttatggcata cgttgttgga tgatccttct tcttacgtag
     3781 aatcttctgc aactgcagga tttgcttacg gtattttgaa agcggtccat aaacgttatt
     3841 taccacaaga atataaagaa gtcgcttatc gtgctatcca aggattgctt gaacaaatcg
     3901 atgaaaaagg agaagttcaa aacgtttcaa tcgggacagg aatgggggac agtttagatt
     3961 tttatcggaa tatcggaatc acagcaatgc cttatggaca gtcgttaacg gtgctttgtt
     4021 tgacagaatt gctcgtctct tattgttaaa agaaaggaaa cccatatgaa aaaaacatca
     4081 ctgctgtttg cagcaagttc gcttagccta ggtctgctag gtctaagcgg ctgcggaaat
     4141 aattctagtg tggcaactgt cgcagatgca gatgtaaatg agatagttac cttgcgcttt
     4201 gcttatgcaa gcaacagcca gccagtaatt gatgcaatga atgaatttgg gcgtttagta
     4261 aaagaaaaaa cagatggttc cgtacagatc cagtatttcc cagatggaca actgggtgga
     4321 gaacgtgaac tgatcgaatt gacacagtca ggagcagtag atttcacaaa agtaagtgcc
     4381 tcagcgttgg aaagtttctc taaagattat tcgatttttt cagttcctta tctattcaat
     4441 gatgaagaac atttctttaa ggtgatggat aatgaagaaa taatggagcc aatctaccaa
     4501 tcgaccaaaa atttaggatt taccggattg acctattatg attctggtca gcgcagcttt
     4561 tatatggtag acggtccgat ccatacgccg gaagatttgg agggaaagaa gatccgtgtg
     4621 atgcaaagcg aaaccgctat caaaatggta gagcttttag gcggttcgcc cgtacctatg
     4681 ggaagtgatg aagtgtacac gtctcttcaa tccaatttga tcaacggatc agaaaacaat
     4741 gaatttgtct tgtatacagc tggacatggt ggtgtggcta aatactattc ctatgatgag
     4801 catacacgtg tgccagatat catcatcatg aatgatgcca tcaaagaaca gctaactgat
     4861 gagcaggaaa aagccattga agaagcagcg aaagaatcaa cggtttttga aattgaagct
     4921 tttgcaaaag caattgaaga agaaaaagct ttagcacaag aagaacatgg agtccaattc
     4981 aatgatgtag ataatacacc gtttcgagag gcagttgccc cacttcatga tatattcaaa
     5041 aacgatccta cctacaaaga cctttatcaa aagatccaag agcaaggagc gtagctgtga
     5101 tgaaaatgat tttaaacaag gtattagaga tcctgggagc tgttttgctt gttgggatgg
     5161 tcgctgttgt tttatggcaa gtattttcca gaacggtttt aggaaatcca aatacggtca
     5221 cggaagagct tgttcgattc ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg
     5281 tgggacaaaa agcacattta gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa
     5341 gtaaaatttt agaagttgtt gttcaaagtt tatttctggt atttgctgcg atgatcatgg
     5401 tgtatggcgg atggaatgct gtcacattga caatggggca gatctcgcct tctttaagta
     5461 ttcctatggg atatgtttat ctgtcagtac ctgtatcggg ggtactcatc attatctata
     5521 gcctgatcaa tctaatcgat acattaagca acaagccagc aattcaagaa aaaatataaa
     5581 aatggaggaa taaaatggca atagaagccg gattagtgtt attttttgta tttgttgtgt
     5641 tgcttgtatt agggatgccg attgctataa gtattgcatt ctcatcgatg atgactttat
     5701 tgttagttat accgtttgat gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta
     5761 tcaatagttt ttcgctgatc gctattccgt ttttcgttct ctcgggcatt atcatgaata
     5821 atggagggat agctaagaaa ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg
     5881 gggctttggc tcatacgaat gtgttaggga atgctttatt tggttccatc tctagctcgg
     5941 ccattgccgc ttctaccgcg attggcggag tgttgatccc tcagcaagtg gaagaaggat
     6001 atgaccggaa gtttgcgaca gcggtcaata tcgcgtcagc tccgacaggt atggtcattc
     6061 ctccaagtac tgcttttatc atgtattcac ttgttgctgg tggtgcttcg atctctgctt
     6121 tgtttatggg aggttatctc gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat
     6181 tcatcgttgc taaaaagaat aaataccctg ttgtcgaaaa aggacagatg gctcatgctg
     6241 gaaaaataat aaaagaggcg attcctagcc ttctattgat tgtcatcatt atcggtggta
     6301 tcttgacggg gatatttaca gcagtagaag cttcagcgat agctgtcatt tactccctgt
     6361 tgattgctat gttttactat aaaacggtaa aactaaaaga tatacctaat atgttgtttc
     6421 aatcagtagc aatgtcaggg acgatcatgt tcttattggc tacttcttcc atgatgtctt
     6481 tcgccatggc gtttaccggt attcctcagg caattagttc gttgattatg ggcgtaacgg
     6541 ataataaatt tattattctg ttattagtca atcttgtgtt attactggtg ggtatgttta
     6601 tggatgtggc gcctgctatc ttgattttta caccaatatt tttaccaatc gtcacaagtg
     6661 taggggttga tcctgtacat tacgggttgt tttcaatcat gaatctgtgt gtaggctcca
     6721 tcacgccgcc agtaggtaca ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag
     6781 aggcaatgct gaaaccgctt cttccttttt atggggttat cttagtcgtc ttgtttttga
     6841 ttacttactt cccgcaaatc gtcatgtggc tacccaatat gctgagttag gagaatagtt
     6901 atgttgaaca ttacaattag aggacatgat ctttccgaag tccaaacgat agaagatctt
     6961 gcagaaaaaa caaaagaaca agggattcat acactgcagc tcgccttagg tctttcgttt
     7021 cctgaaacac cttctggcgc aaatgagatc aattctggga tgggaaatta tgtcaaacga
     7081 gttttagaaa agcaagaagt gaccgtagga attttaagtt gctatatcaa tatgattcat
     7141 ccagatttaa cgatacgaca agagttgctg acaaagttcg agcagtatct tcgctatgct
     7201 tcttcctttg gtgcgtcgat ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa
     7261 tatacggaag aaaatttcac agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc
     7321 ttggtaaaag ctggagaaaa ataccaaatg atggtgggga tagaaccagg tttgaatcat
     7381 ccggtgtatt ctttagcaag ggtagagcag ctgatccaag cagtagattc ggactatcta
     7441 ggaatcattt tagatccgac caatttgatc acaagtagtg attatcaaga acaagtccaa
     7501 ttggtagaag aagcttttga acgttttggt gaaaagatat gtgcggttca tttgaaagat
     7561 tttcgtgtcg aacaggaaaa aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac
     7621 acaaaaatca aagaaatcat catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa
     7681 acaaaagatg atgggattcg ttatggaaga agtcttcttg aataatgtaa agaagacgtt
     7741 ggttaactaa tatttagaat ggagggggcg gggctagaag atgccacgtc tcttcttttt
     7801 tataagagta tataaaagca gctctttttg attacaaaaa acgtggtata ataaaattgt
     7861 gcataagtga acaaaaggag gtgcggcaca tgctccaaaa gaaaatcagc ggtgaacaag
     7921 tcaatacgat ggtttctgat cagtatgaaa tctatcatgt caaagatgtc gtcagccaaa
     7981 ataaaacgat ctatcatcat catgatttct atgagatcca tgctacatta aagggagaag
     8041 cgacttttta tttgaacgga cgacaattta cgatcaaagc aggaagtgtg ctgctcatcc
     8101 attccaatga tttgcatcgt atcgtccgcc aatcttcaga tgtattcgag cgtgtctata
     8161 tctttgtcac cccttctttt ctagaaagtc gttccacgcc atggtctaat ctatcggctt
     8221 gttttcaacc aatgggagag aagaggagtc gtgtattgca aatagaacag gaagtattga
     8281 agcaaaaatt atcatttatc gaccatcctc ctgataaaaa aatttacggt gaagacataa
     8341 aatatgaaca agctctggta gaatacttga tttttctcaa tcaagttgta caaaaagaag
     8401 agagtgcgca agaacagaat gctgtagttc ctaatgagcg tgtggaacaa atgttacaat
     8461 atatctcaaa aaatttatct catccgcttt ctttagagga aatggaaaag cagttttttg
     8521 tcagcaaata ccatatcaca cgagaattta aaaagtacac tggatttacg tttcatcagt
     8581 atgtaatgaa gaaaaagctg atctatgcca agcacttgtt gcgagaatac cgcagctcta
     8641 gtgctgtata tagtaaaagt ggtttttctt cttatccgca ttttctgaaa gcattcaaga
     8701 gagagttcgg cataacgcct aaagaatttt tgaaacgtga tacacagaaa cagttggttc
     8761 attatgaaca ttttgaagat gaacaatgag aatgatcact aaaaaataac cgcagtagct
     8821 gctgttgttt acatagaagc agaaactgcg gttattttat ttttatctat atatttcaaa
     8881 atattttgaa aaaatgacgt acatattata tgaatcagct gaatttacga aagtctctcg
     8941 gcgatagtcc tgtttttgct ttgaacaggc ggctaaagta agcaaaattc tcaatcccta
     9001 tttcttcgga aatcattcga accgtaaatt ctgtataccg aagaaggtct ttagcgtaag
     9061 aaagtttttt gctgatcaaa taatcgattg gtggagaacc gataaatttg gaaaaatctt
     9121 tgctcagctg atatttattc aataagaatt gttgttctaa ttgatcaagt gtaatcgttt
     9181 ctttgaaatg ttcgtctaag tattgcttca gtttgtaaac gtaggcggga atatcctctt
     9241 cttcgaaatc tagctgatac ttttgcaaaa tcagttcatt cagcaactcg tggatagcta
     9301 cagaggactg gaaatcggta cgggcattcg tgccttgttg catgtggatc aattctgtga
     9361 tcaaatggtg gatcttgttt tctgatggat cccctgttga atggaaggtg ttatggccgt
     9421 cttttgtgaa ctcctgataa aatgattgga cgttctgacc attcaaatgg atccaatcca
     9481 tttcccatgg ttcttcgctg atcgtttgat aacgctgata ttcttggcag tcaataaaaa
     9541 ataagtctcc agcttttagc tgttgctgat tatcattata tgtaagcgaa cctttgccag
     9601 acaaagtaaa ctttatcaag taagaaggta aatgtgctcg ttcagtatag taagggattg
     9661 gtgctttgaa gtaaccgatt tcttggatat aaaaaaataa attcctgctt ctttgtgtag
     9721 gcgtcaagat cactcttttt gagtcttccg accatgagtg gttggctttt tcccagtaat
     9781 ccatgtaaac actcccaact tgtcaagata atgcaattat ataccaaaat agtgtgaagc
     9841 aaaatataag attctgtatt atgatagcat tgttaataaa tgataacgct ttattaataa
     9901 agtgctaatc aacaataaag taaaagaggt ggagaaatga atccaacatt tgttgccatc
     9961 gatatcggtg catcaagcgg acgactaatg aaaagtacga gggatgcaaa aggttctttg
    10021 atcttggaag aagtacaccg gttcaaaaac ggctttcgga acaaagacgg tcacgatcgc
    10081 tggaagatcg attatcttat caaagagatt ttgattggat ttgaaaaatt aaagcagaca
    10141 gggattgatc gcctttttgc ggggatcgat acatgggcag tcgattactg tctgattgat
    10201 gaacaagggg atcggataaa tgatccgatt gcttatcggg acaagcgtac caatggagca
    10261 gtagaacagt tttctgaaat ctattctcta gataagttgt atgagcagac agggattcag
    10321 atccagcctt tcaatacctt gttccaattg tttgctgaag aaaaagaaaa tttggcaaaa
    10381 gcacataaat tgttactgat acctgattat ttagggtatg tctttactgg taacatggtg
    10441 acggagaaaa ctaatgcatc tactatgcag ctgctgaatg cagggacaaa agaatgggaa
    10501 atagaacttc ttgaaaaaat cggtgtctcg aaggaattat ttccaccatt gacagagccg
    10561 ggaacgatac taggagatct gcaaaaagaa caattttcaa aatatgattt gccagatgtg
    10621 acctttatca cagtagcaag tcatgatacg gcgtcagctg tattaggaac gccaggtcaa
    10681 ggagaacgtt ggggttatat cagcagtggg acatggtcgc ttttagggat tgaaacaaag
    10741 atcgccaatg tttctcgcaa tgctttttct gaaaattata cgaatgaatg gggtgcccat
    10801 aacacggtgc gttttttgaa aaacatcatg gggatgtggt tgatccagga agttgcccga
    10861 atgcaagagt acgagtacag ttatgcggaa ttggcagagc ttgctgcaaa agaaaaaccg
    10921 ctgcagcaag tgatggaaat caatgaccct cgatttttga atcccccaaa tatgatagaa
    10981 gagattcagc aatactgttt tgaaacagag cagccagtcc ctgtctcatc gggagctatc
    11041 gctcgttgtg tgtatgataa tcttgctctt tgttacgcac aagagctgat caaagtagaa
    11101 cacttaactg gaacgaaatt ggaaaagctg cacatcgtag ggggcggatc gaataatcgt
    11161 ctactgaatc aattgacagc agattatgcc ggaatcattg tagaagcagg acctagtgaa
    11221 gccacagcaa tcgggaatgt tgccatgcaa atgctcagta caggtcagtt ttcgaatata
    11281 gaacaagcaa gaaatgtaat caaacagtcg tttccatgtg aaacttttta cccagatgaa
    11341 aataagcaaa acacgatgga atggtacgtg aataaaatca aaggagtaat cgtatgaaca
    11401 cgattgaaag aaattacgaa caagcaaaag aaaaatatgc aactatcggt gtagatacag
    11461 atgctgtact agaaaagata caggacatca aaatttctat gcattgctgg caaggagatg
    11521 atgtcaaagg tttcttgaca ccggatggcg aattgaccgg tggaatcatg gcgacaggga
    11581 attttccagg agccgcacga acacctgaag agcttagaca agatctggag aaggcttatt
    11641 cgttgatacc gggcaaacac aaattgaacc ttcatgctat ctatttggat acagaagaat
    11701 ctgtcgatct aaatgaaatt gagccgaaac attttgaaaa atgggtagag tgggcaaaaa
    11761 aagaagaaat cggtcttgac ttcaatccga ctttcttttc gcatccaatg atgaaagacg
    11821 gctttacatt ggctcatcca gataaagaag ttcgtgaata ctggatcgaa cacgggaaaa
    11881 gaagccgcaa aatcgcggag tactttggga aagaaactgg gcaaacaagt atcaataact
    11941 tttgggtacc tgatgggatg aaagataatc cgattgatcg ttttacccca cgggaacgtt
    12001 taatggaatc actcgatgaa atttttacag aagagttaga tgaacggtat gtacaagatg
    12061 cagtagaaag caaactattt ggtttaggcg ctgaagctta tacagtcggt tcccatgaat
    12121 tttatatggg ttacgggttg actcgtgaca aacttgtttg tctagatgct ggacattttc
    12181 atccaacaga agtgatttcc aataaattat cttcattagc tttattcagc aaaggaatca
    12241 tgttgcatgt ttcccgtcca gtgcgttggg attcagacca tgtggttttg atggatgatg
    12301 aattgcagga aatcgctaaa gaattagtcc gtaacgaatt gttggaaaaa acaaatatcg
    12361 gtctggattt ctttgatgca acgatcaacc gtatcgctgc ttgggtcatc ggtacgagaa
    12421 atacacaaaa agcactgctg aaagctatgc tggaaccagt tgagcgattg aaagaaatgg
    12481 agctggcttt tgactttact tcaagaatgg cttatacgga agaattgaaa gatttcccgt
    12541 atgccgatgt atggaattat ttctgttaca aaaatcaagt tccagttggt ttggattggc
    12601 tggaagaagt ccaggaatat caaaaagacg tgttggaatt aagaaaatag gagtgtggct
    12661 atattgaaaa aaatagatat tttacaagcg ccttatgtga aagaaatgat cgaaacgacg
    12721 tcgaatcttt atcgcttggg atgggatgaa agaaatggcg ggaatatttc ttatttacta
    12781 aaagaagaag agatcacacc gttcttggat cctgaaaaag tgatccgccg catctcgatg
    12841 atttttgatg ccagccgttt agcaggatgc tattttattg taactggttc aggaagatat
    12901 tttaaaaatg tagcgagcga acctgccgag aatcttggaa tcgttcgagt tgctgaagat
    12961 ggcaaaacac tggaactttt gtgggggctg gaaaacgaat gccttcctac aagcgagttg
    13021 ccaagtcatt tcatgagtca tatcgcccgt ttagaagtag acccagataa tcggatcgtt
    13081 atgcattgcc atgctagtca tctattagcg atgagtttta cgcatgaatt agacgaacgt
    13141 tcctttagtc gtacgctatg gcaaatgtgt acggaatgct tagttgtttt tccagaagga
    13201 atttccatca ttccgtggat ggttccggga acaaatgaga ttggggaagc aacagctgaa
    13261 aaaatgaaag aaactcgatt ggttttatgg ccacaacatg gaatctacgg gtcaggaaaa
    13321 gacatggatg aagtctttgg tttgatcgag acagcagaaa aggcagcaga agtctatacg
    13381 tacgtcaaag cgcaaggacc aatcttgcaa acaatcacgg acgaaaacct gtggcattta
    13441 gcagatgcgt ttggtgtcac tccaaaagca ggttatttgg aggaagttca tacgaaagca
    13501 ggtgtctgac atggtgaaaa aagctttttg catgaaagtt tacccagaaa aacaagaaga
    13561 atacaaaaaa agacatgatg aactttggcc agaaatgcgt acagcacttc gtgaacacgg
    13621 ggtcatcgac tattcgatct atcttgatcc gagtacaagt ttgttatttg gttacttagt
    13681 gatcgaagat gaaaagttat gggaaaaaat ggcaaataca tctatcaatc aaaaatggtg
    13741 gaagtacatg gaagatatca tggagacaaa tccagatgcc tctccagtca gttgggaatt
    13801 gatgccagtc tttgaactgt agcaacaagc aactgggcaa ccggttgctt ggggttcttt
    13861 cgtttttatt gtgaaaaaaa atacaaatta ggaggaatag aaaatgagta atcgaatgat
    13921 tttaaatgaa acatcgtatt ttggaaaagg agcgattgag tcgatcgtcc ctgaatttca
    13981 gcagcgagga ttcggcaaag cagctgtcat tactgacaaa ggattgatcg agcacggtat
    14041 agcaacaaaa gtaacagaac ttctagataa agcagcaatc gcttatgctt tatacgacgg
    14101 gatcgtacct aatccaacga tccaaaatgt aaaagatgga gtggcttttg ttaaagaggc
    14161 agaggcagac tgtttgatcg caatcggtgg aggttcgcca atcgatacgg caaaagccat
    14221 tgggattatt ttgacgaatc cagagttttc agacgtcatt agtttagaag gtgttgctga
    14281 tacgaaaaat ccatgtttac ctatattagc tataccaacg acttctggaa cagcagcaga
    14341 agtaacgatc aactacgtca tcacagatga agtaaatcac cgaaaatttg tttgtgttga
    14401 tcctcatgat atcccaatcg tcgcgttcat cgacagcgat atgatgatgg gaatgccgaa
    14461 aaaattggct gcatcaacag gtatggatgc tatgacgcat gctattgaag ggtttatcac
    14521 aaaaggcgct tgggaaatga ctgatatgct gcatttaaaa gcaatcgaaa tcatcggcca
    14581 ttctttggaa gtttctgtag atggcgatca aaatgggcga gaaaaaatgg cattaggaca
    14641 gtatattgcg ggaatgggct tctcaaatgt cggtttagga ctggtacacg ggatggctca
    14701 cccgttaagt gcatggtaca acattcctca tggcgtagcg tgtgcagcac ttttgccaac
    14761 tgtcatgaaa tataacaaag aatatactgg agaaaagtac cgtgagatcg ctcttgttct
    14821 agggattaaa ggagcagctg aaatgtcttt agaagatgta agagaagcgg cttgtggtga
    14881 gatcgatcgg ttgagcaaag cggttggaat cccagaaacc atttctgaat taggtgtaaa
    14941 agaagcagat attccagcaa ttgcagaaga tgctttgcga gatgtatgca caccaggaaa
    15001 tccaagagaa acgacagtag aagaaatcat tgctttatat caaagtttga tgtaaaaacg
    15061 aagcggatct tcaacattaa attgcctgta aagagaaatg gtagtttata tctggatatc
    15121 tcttttaaat tgatggtatt tccgctataa tttttcttat aaagaaatag gagaatatta
    15181 tatggaaaat aaaaagcctt atggaactgt attgatcaaa gcctctaaaa tactagacta
    15241 tttagcggaa aaccaagatg tctctttgca gacgatcgca aagggtgtgg gaatgacctc
    15301 ttctacagtc ttgaaaatac tagatacttt gttgatgatc ggctacgtaa ataaaaattc
    15361 agagaaaaac tatcgtttag gtgcaaagct tattcgctac gcgaataaaa acattgaaca
    15421 aatcgatttg gtagagttga ctttaccgtt tttagaacag ctgcagcaaa agatcgatga
    15481 gactatccac ttaggggtat tggacaacaa tgagatcttg tacgtgaaca aactagatcc
    15541 aaaacatcaa acgatccgca tgtcttcaaa aatcggaatc acccgaccat tatacaattc
    15601 tgcgatgggc aaagccgtat tagcagaatt cagtgaagag cagtacactg attatcttga
    15661 aaagcatccg ttgatccctt acacagaata tacgatcacc aatgcattac ggttgaaaaa
    15721 ggaaatccaa acggtaaaag aaacccacat cgcatttgac gatgaagaaa tcgaacgtga
    15781 tattttttgt ataggagcct caatcgtgaa aaacgaacaa atcattggtg catttagtgt
    15841 aagtatgccg aaatatcgtt taacagatga gacaaaagaa caaataatca gtgccctcaa
    15901 gcaaacaaaa agcgaaatcg agcaaagaat tcaaaaataa tgaaaaaagc gtttgcaatt
    15961 tttcttttct gccgtataat atgggtatag atttccaagt tataaaataa aatttctaaa
    16021 taggaaatag gtggagaaga tgaaaaaaat tgatatttta tctcgattga aaaacgccgg
    16081 agtgattgcg gtggtacgcg gaaaaagcaa agaagaagct ttgaatgctt gccacgcaat
    16141 catcaaaggt ggtctaactg gtattgagtt gacttttaca gtccctcaag cagatcaagt
    16201 aatcaaagaa cttttgtcat tctacaaaga ccaaccagaa atagtgattg gcgcaggcac
    16261 agtattagac gctgtcacag ctcgcttggc tatcttagct ggcgcagagt atattgttag
    16321 cccttccttt gatcaagaaa cggcagagat gtgcaatctg tatcaaattc cttacttacc
    16381 aggatgtatg actattaccg agatcaaaac agcgctgaaa agcggggtag atatcgtcaa
    16441 actatttcct ggaagcgctt atggaccaag tatcatttct gccttcaagg cgccgatgcc
    16501 tcaagtcaat atcatgccaa ctggcggtgt gagtctggac aatatgaaag agtggttcga
    16561 tgctggtgtc gtaacagtcg gagtaggtgg gaacctttta gcacccgcag ctacaggaga
    16621 ttttgacaaa gtaacggaag tcgcgcaaca gtatgcagca aaaatgaaag aaatcaagag
    16681 gtagctgctt atgggaaaag tagtgacact cggagaaatc atgttgcgat tgtccacaga
    16741 gcagggaacc cgtatcgatt caagtgaacg attcgctgcc cattatggcg gaggggaagc
    16801 aaacgtagca atttctttag ctaattacgg acatgaagcg atttttgcta caaaagtacc
    16861 tgataacgca ttgggaaaag cagtcaaaaa gcacttgcaa tgttatggag tagatacgaa
    16921 acaggtatta tctggtggat caagactagg tacttattat atggaaactg gtagtggcga
    16981 acgcgccgcc tcagtgatct atgatcgtgc cggatcaagt tttgctgaaa tggatcattt
    17041 ggaatggtcg ctagaggagt tattccaagg aatagatata tttcatgttt caggaatcac
    17101 cccagctttg tctaaaaaat ggcaaaaaat gacgaaacag gtgatagaag ctgcaaaaaa
    17161 agctggctgc ctgatcagct tcgatatcaa ctatcgtgga aaattatgga cacagcaaga
    17221 agccagcgaa gtaatccatc agcttttacc tttagtagat atctgctcag ctggaaaaat
    17281 ggatgctctt tatttgttag gtatcgagaa agcgccagaa gcagaagaac aacctctaat
    17341 ttattattat caaaaaatgc aggaaaagtt cccgaatatt caggtgtttt attcgaccaa
    17401 gcgtaccgtt cattcagcaa gtgaaaatga attgaagggg acattgtgga tgaaccatga
    17461 atatgtggaa tcccaagtcc atagaatcac cccaatcgtg gatcgagtag gtggcggaga
    17521 tgcttttgca ggtggcgtgc tccatgggat tttatccaat atgtctccgc aagaaatcat
    17581 tgattttgct acggcagctt ctgccttgaa gcatacgatc catggggact gcaaccaatt
    17641 tagtcaagaa gaagtcgcac aatttttagc atgcggctct ggaaaaatca ttcgatagaa
    17701 ggagtagaag aaaatgcaag aaatggaaac acgttataca cacagtccag aagatatccg
    17761 tcattattca acggaacaat tgagaaaaga atttttagta gaaaaggtat ttgaaccagg
    17821 gaaaatcagt ttgacttata cgcacaacga tcgaatgatc tttggtggag tgacacctac
    17881 tgaaaaagaa ttagaaatca cattagacaa agaactaggt gttgattact tcttagagcg
    17941 tcgtgaacta ggtgtgatca atatcggtgg accaggattc atcgagatcg atgggcaaaa
    18001 agaagaaatg aaaaaacaag acggatacta tatcggaaaa gaaacacgac atgttgtatt
    18061 ttcttctcaa gatgctgaaa acccagctaa attctatatt agttctgttc cagctcatca
    18121 taaatatcca aatgtaaaaa tcagtatcga ccaagtaaaa ccaatggaga ctggtgaagg
    18181 cctgacactg aatgaacgta aaatctacca atacatccat ccaaatgtat gcgaaagctg
    18241 tcaattacaa atgggttata cgattttaga accaggaagc gcttggaata cgatgccttg
    18301 tcatacacat gaacgtcgta tggaagctta tgtctacttt gattatgcca atgaagatac
    18361 acgtgtgttc cacatgatgg gcaaaccaga tgaaacaaaa catttagttg tagacaatga
    18421 acaagccatt atttcgccaa gctggtcgat ccattcaggt gtaggtacaa gcaattactc
    18481 attcatctgg gcaatgtgcg gagaaaatat cacctataca gacatggata tggtgcaaat
    18541 ggatcaatta aaataagcat aagggagaga aaatgatgga atttagtatg gatatgtttc
    18601 gtttagatgg aaaagtagca ttagtcaccg gtgctgtcta tggtattgga tttgagattg
    18661 cacgctcttt agctacagca ggagcaaaaa tcgtatttaa taacttgaat caagaatcag
    18721 ttgatgaagg tatagcgcat tataaagaag caggcatcga tgcaaaaggt tatgtctgcg
    18781 atgtgaccga tgaagaagcg gttcaagcga tggttcaaca gatcaaagaa gatgtaggtt
    18841 cagttgatat tctagtcaac aatgcaggga tcatcaaacg gacaccgatg atcgaaatgg
    18901 atgcggcaga cttccgccaa gtaatcgacg tggatttaaa tgccccattc atcgtttcaa
    18961 aagcagttat tccggatatg atcgaaaaag gcggcggtaa aatcatcaat atctgctcca
    19021 tgatgagtga attaggacgc gaaacggtca gtgcttatgc tgcggcaaaa ggcggattaa
    19081 aaatgctgac gaaaaacatc gcttctgaat acggtcaata caatatccaa tgtaatggaa
    19141 ttggaccagg ttatattgct acaccgcaaa ctgctccatt acgcgaaaca caagaaaacg
    19201 gtgaacgtca tccattcgat caatttatcg ttggacgtac gccagcagca cgctggggcg
    19261 agccaatcga tttggctgga ccatctgtct tcttggcttc tagcgcatct gatttcgtta
    19321 acggccatgt cttgtatgtg gacggaggca tacttgccta tatcggaaaa caaccttaaa
    19381 agagctttag tacactgttt agtggagagg ttgtgaaatc agcggattga acgaataccg
    19441 aaggacagct gattaaacat catttattcg aaaaaagagg ctgggacaga agcatttaac
    19501 tccaagaaat aagaaggaat tcacgaaaat tgcttttcaa atttttgtga atttcagctt
    19561 atttccgaag gagttgcttt tacttccgcc gtttgtacgt gtttagagcg tgagacaaaa
    19621 gtgttcttta cttttgtccc acgctctttt ctgtgcgtct tccatacttc actaagtcga
    19681 tccctcttgc ttcgcaccaa tccttcactg gtccggaaaa gatcagagat tgttggtgta
    19741 gggccgcggt attcgttgcg ccgaccatag tataaagcag acgaagctct tcttgccatt
    19801 gcttcatcag tataatagtt tcttctacgc cgtgattcat cagatgcgtc agtacggttc
    19861 ctgaagtacc cacggcattt gcgccgagac aaagggcttt aaatatgtca taggcattgc
    19921 ggatacctcc ggaggctagg atttccatag aagtgtctgc ttcatttgct tcaagcaatg
    19981 aagagactgt cgattgcccc cagtcagcta aataactcag ttcccgcttg gagcgacggg
    20041 cgttttcgat ctgggtgaag cttgttccgc ttcgaccgct gatatcaact gtatgaactc
    20101 ctaaagcagc tagatcattg agcgtttctc ttgtcatgcc aaaaccgact tcttttacga
    20161 tgagggggac gtcaatggct gtttgagttt cttgaataag tactttccaa ttggtaaaat
    20221 cacggtctcc ctcaggcata accaactctt gaggtgcatt caaatgaatc tgtaaggcat
    20281 ctgcgtgaaa caatcggata gcttctttgg ctctttcgac agaagtccct gcaccgatgt
    20341 tggcaatgat tttcccgtga ggatattctt gtcgcataat cgtataagta tcggctaaag
    20401 aagggacttt caaggcagcg ctgactgaac cagtggcaat catcaaatct gcttctcttg
    20461 cgacgattgc aagatcttgg ttgatttttt ttgttttttc gcttccgcca gtcatcgcat
    20521 tgatataaaa tgggctgctt agtgtaaacc caactgcctg tgtacttaga tcgacatcag
    20581 aaactgctat ttgcggcaaa gggttatgga tgatccgtac gaagtcaaat tcgttttttt
    20641 gtttatcatg aaaggcttta gctagtgaaa catgttcgtc ttttcgattc atcggttttc
    20701 ctttctttga tcgctataga catgtaacgg cagtggagtg atttctgctt tttcccatgc
    20761 actcattaaa ggaagaatgc cagatttctg gtcaacaatt acgattccgc aatcgccccc
    20821 acctgcacca gaagattttg cggctccttc atactgttca gctaaattac acaatttgtt
    20881 caaagcaggc gtttcgatga cgaccccagt gattgcagat aaatcatgca gtaattgtcg
    20941 gttttttcga atcatctgtt ggatcaacgt tacattattt tctttaaacc ctttgatcat
    21001 ttcgttgaca cattctgtac tattttttaa gaaaagctga taagccacca ttttatcttc
    21061 tctcgaacgg tgaacttgat cgaccaaatc agaagtagag gcagggctac ccgtccaacc
    21121 aatcaataaa cgtaaatctt caggagcaat caatggctca atggatagac ctggccaatc
    21181 cagggcaagt aactcactga tagaatgctg agtttcttgt tcttggagcc aaggatggtc
    21241 gaaggttgag aaagcgatcc agccaccata acagctagca gcgatgtcgc cgcaggaacc
    21301 attatcttga actgctaaat tggctagtgc tgcaatcttg aaaatctcca actgagataa
    21361 attcaacgcg taaaaaacat tcaaggcttt gaccgttgca acggtcacgg caccgctaga
    21421 acccaatcca tatttccggc catttgaact gtctaactca cttgttactt tcagatcata
    21481 aaaggataaa agaatgtttt tttcttgtgc atacttttca gtcaagcgaa tagcagcaag
    21541 gatataatga aaaggatttt cccgaatatc taaaactaat tctccgttgc gtcttgtcca
    21601 acgtacaggc atcccactat attgtgcaga ttggatactt ccgacttttc gtgcagattc
    21661 tacagtgact gtcacgaatt gatcgactgc agcgataact gctggatggc ctgtttcaac
    21721 aactgcatat tctccggcaa tatagagttt gcctggtgca gatacttcaa tcataaaaat
    21781 ctccctttat tcaataatcg caattcctgg accagcatgt gcagtaatta cttgttcttt
    21841 gctaaataat ttggtaaatg tttcttgtac ctcagataaa tggctgtttt ccactaaaac
    21901 tttgacatta gggccagcat ccatcgtaaa gtagcatgga atgccttgct ttctcaattg
    21961 tcgcaccgca tccatcgctt ttaagctgtc aggagaccag taagtgaatg gaggctgggc
    22021 agctaaagtc gttccatgca tttttagtcc gtttctttcc atcgtttctc cgagaagttg
    22081 gaaatctttt gttttgattg cttgtttcaa ttgatataga tccccttcaa cggaatctaa
    22141 ccagccttga taaaaactgg atgtttcgac tgtccgacgc atcccatttc tgctggacac
    22201 ttcttttttc tggtcgttga tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc
    22261 ggaagggact ggcttagcgt aagaacttaa gtcgtcatgg cctttttccc attcgacgaa
    22321 accaccgaaa atacttcggc aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga
    22381 gagatcatct aatccaagct taagagcagt attgcaagcg cctgctaaag cagctagccc
    22441 gctggcagag gaggctaatc cagctgcggt aggaacgaaa ttctgactga ttactgaagc
    22501 ttttagtgag aggccggctt ctttacgaaa aaggtcaaga aactgactga ctttttttgt
    22561 tgctttttcg tcttgcaagg tgccatctag ataaaattca tctaccatat agctatcaga
    22621 aaagatgact tctgtctctg tataaaatgc atccaatgtt aacgaaaggc tgttgttcat
    22681 tggaaggatg agttcttcat ttttcttacc ccaatatttg attagagcaa tgttggtata
    22741 tgcgcgtgct ttgcctttaa acataggtat gtactcctaa tccttgtatc caagtttcag
    22801 cagcacccgc atcttcaagt gcttgactga tttcttgggc ttttgttttt gtttgtgcta
    22861 atgcaatcat acagccaccg cgtccaccgc cagttaattt ggcacccaat gccccggtat
    22921 cttttgctgt ttggatcaat agattaagaa aatcattgct gattgtcagc ttttccagaa
    22981 gtgattgtga ttcatccatt gtctgtgcca acgtttctgg tgaattttca atgattgctt
    23041 gttttgcttg cttcgtcaag tatcctaatt tttggatcat ttgtccagtt tcatgaggct
    23101 gggtttcaaa gaggtgagcg acatctttga cggcttctct tgtttgtcct ttgattcctg
    23161 tatcagcgac aatcaaaaaa gcatcgatgt tcaaagaaaa gtagtcgaaa ggatgccctt
    23221 ttgtaaaata aatcggctgc aagctgctag tagcggctgc atcgattcca ctaggattac
    23281 cgtgggcgat tttttccgaa agctggacat tttctaatag tatttcacga gacaaaggaa
    23341 atgctaagta atcataaaaa gcacgagtga ctgctgtggc gactgcagcg cttgatccca
    23401 ttccacgttc agcaggaatc gtgctttcaa tggtcagttg caagtttttt tggatgtcat
    23461 gctgtttttt taagtgaatg aataattttt tgatattttt taatgcatga ggagcttcgg
    23521 ctaaatttcc tgaatagtag gaagaaacca gttgatcgtc cattgcatcc agctcttcaa
    23581 ggaatgcggt gacttttgtt gcatagaaag gaaaggcaat cgctggttct ccataaacaa
    23641 ccgcgtgctc gcccatcaat atgatctttc cgcttgattc tccttggcca tagtttgcca
    23701 taatctaact ccttctcacg taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg
    23761 agttgcgctg cttcacgaat ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt
    23821 attttactaa aaccatctca gaagtaaaac taagaatacg ttttctaaag aaaaaacact
    23881 tttttcttaa gaattttttg actttttttc ttctttgact agaaaaaaat acaattgaca
    23941 ctactagata ttgggggtat atttaaaaag gttggagtta aacacaatat ttagtgatga
    24001 agtgagctgg aggtaaatga gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc
    24061 aagtttaaca gaaatgatgg ctgaacactt tgatagtcag gcattttatg aatcgattga
    24121 tgataatgaa gtcttgccct tattttatgc aaaccctgaa cagtatgctt ttttgctgca
    24181 aatttatttt ttgaataaac gttttgcaag catcaaacaa gcaatgaaag atgacaataa
    24241 tgtattggac cgttcgattt atgaagattc actgcttttc catctaaatg cagatctagg
    24301 aagagcaaca gaaacagaag ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga
    24361 acttccctat gctgcccata aaaaacatcc ggatttatta gttcatattc gtgtatcttt
    24421 tgatacgatg ttggagcgta tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga
    24481 tccaagtcta tacgattatt acaaagaatt gaacagacgt tacgatcaat ggtatgaaga
    24541 atacaaagaa agtccaaaaa tccaaatcga tggagaccgc tataattttg tggaagatcc
    24601 tcaagcaaaa gaagaagtct tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac
    24661 caaagtagcc atttgattta cagttgctca aataaagggt aaacctttat ttgagcgact
    24721 tttttcttta tactggaaac aaatgtcagg aggatagagt atgcgggaaa aacactattt
    24781 taatcaagac cgtaattttt ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa
    24841 cttaaaagaa gaatatggca ttagtgacga gatgctgact tattctctag ataaaaatga
    24901 acgggcgcgc gtagagtacg attcttttga tgaagcgtta ctgcttgtat caaatgttcc
    24961 gcaccagcaa aaagtcgata atcattatga aactagtcct atcgctttta ttttaaaaga
    25021 agacggtttg tttacattta ccacgccgaa taccgagtac gtgattcgac taattcgatc
    25081 gcttttggaa cgaatgccag acatgagtgt atacagttta ttgtttcgta cattgttttt
    25141 gatttctgat tccttttttc ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa
    25201 tctgaaatta agagaaaaaa caacgaacaa gaatctgctg caactttcag atttagagat
    25261 cggtttggtt tacttagtta caggaacaaa acaaaatgta gtacttttag aacaaatcaa
    25321 ggcattggct atttatcgca agttgtcaga aaaggaaaaa gaagagttgg acgatgcgct
    25381 gatcgaagca cgacaagcag ttgaaatgac gaacttagct tcgcagattt tagaccagct
    25441 ctctggcact tataataatt tgctgaataa caacttgaat gacacgatga aatttttgac
    25501 ggtctggtcg ctgatcttga ccgttccaac tattgtgaca ggattcttcg gaatgaatct
    25561 ccagttaccc tttacccatt ccgttttcgg atggggaatc gccttgatca tcagtttggt
    25621 cttatctatt tggatgctta ttgcactgtg gcggcggatt cgttgatcta gtctttgatc
    25681 tgcaaaccaa gggcttgggc gatcacaagt gcttgctctt ggttgacgat tagtcccgcc
    25741 aaagaatctt gagataaagc gattgctgtg aagaaattag tcgtaaagtc taatcccttc
    25801 agagaagttc gaaaccagtt cgaaccggtc aactgattgt cagacaaaaa aaggtttttc
    25861 caagtgacct cataaaattc gctgtcttct aattgacaat gatcgaagga gacagctttc
    25921 aagtttgtac tactaaagga agccatcgaa gcaatacaat cagtgaaagt acagtctcga
    25981 agataacttt cggcaaaatt cgtacctgtt aatttacatt gatgaaacac gacttggtgg
    26041 aaactgccac caatccactc cagattggaa aaatcacatt tttcaaaaat gacattactg
    26101 cattcaaaat gacgtaaaac tgttttttgc atcgtaagtt tttggatatg actctctctt
    26161 aataccatat ttttggcatc gagatagctt tggtcacccc cgttgatcag aagaccagag
    26221 aagatctcct catcttctaa ataaaactct ccctttgtaa gaagaggaag agaaggtgga
    26281 ataggttcga tttttttcat gtgggttcac ctcgtttttc tttagtatag caaagaagcg
    26341 ggaatcactg ctagaaagtc tgctaatcta atcattttca gaaaaggaag acaaactgta
    26401 tgaaaaacat tgacaaaaag attagttttt gctatactat gatggttgag aaataaaagc
    26461 agaagcaccc gcttctcgcc ttagttgata atgtcactgg gctagatagt tttcactggt
    26521 tctattaggt gaagtacgcg cgggttctgt aagagaactc gtttttttat tgccgtttat
    26581 tcggtaagct tttctttctc tcataatatt tggaggtgaa tgaccatagc aaaggatatg
    26641 atggttaacg acggcattcg tgcacgagag ttacgattga tcggttcaga tggtgagcaa
    26701 ttaggagtta agacaaaagc agaagcattg aatattgcag aacaagcaaa cttggatctt
    26761 gtgttagttg cccctggtgc gaaaccacca gttgcgcgaa tcatggacta cggtaaatac
    26821 cgtttcgaac aacaaaagaa agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta
    26881 aaagaagttc gcttaagtcc tacgatcgac ttgaatgact tcaatacaaa acttcgtaat
    26941 gcacgtaagt tcttggaaaa gggcgataaa gtgaaagctt ctatccgttt caaaggccgt
    27001 gccattaccc ataaagaaat tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg
    27061 gatattgcaa cagtggaaca aaaagcgaaa atggacggac gcagcatgtt cttgacgctg
    27121 gcaccgaaaa acgacagtaa gtaatttgaa tgactaactg gctgttggac agccgatagt
    27181 cggataacga aagattttga ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg
    27241 gatcagcaaa acgtttcaaa cgtactggta aaggcggatt gaaacgcttc cgcgcgttta
    27301 caagtcaccg tttccacggc aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca
    27361 tggtttctag tggcgacttc aaacgtattc gccaacaact agcaaaaatg aaataagaag
    27421 tacaaataac agaatctgaa gatttatttt tattaggagg aattatacat ggcacgtgtt
    27481 aaaggtggaa cagtaactcg taaacgtcgt aaaagagtgc taaaattagc aaaaggttac
    27541 tacggttcaa aacacacttt atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac
    27601 tacgcatatc gcgatcgtcg tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt
    27661 atcaacgcag cagctcgtat gaatggcttg agctactcaa aattaatgca cggtttgaaa
    27721 gttgctgaaa tcgacatcaa ccgtaaaatg ttagctgact tggctgttaa cgatgcagca
    27781 gcatttactg cattggctga ccaagcaaaa gaagctttgg ctaaataagc taaacaatta
    27841 taaagaggaa agaagataac gaaaagttat gttctttcct ctttttttat ttacaatcct
    27901 ccatcaaatg aaacaggaca ataacgtata agtctaaaat agcataatta aggtttttta
    27961 attgaaaact gaacatttat ggtcaccctt taatgaaagc gctaactaca taagttagaa
    28021 tgagtttgac tgatatatta gtttgcttat cagaaaaaca aaatggacgg aggaataaca
    28081 tgagtgatat tattattggt attttgttag ttatctcatt ctttttcatg gtctggtact
    28141 gtgtaaaagg ctacaacttg atggtcggct ttgccatcat ggctactgta tggatgggat
    28201 tagcgctagt cggaaacacg ttttcaccaa acccggcgat ggaagggcaa ggagtaattg
    28261 atgtgttgac gcatatctat acgacggggc cagctgagta cgccaaatcg atcttggtaa
    28321 atgtcttctt tggtgccttt ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc
    28381 tgattcgaaa ggtagttgaa cttggagggg acaagccaag aatcacgatg agcttactat
    28441 gtattgtaac cgctgttata ttcatgtcga tgacaggaat cggaccagtg atttctattg
    28501 cagttattgt gttgccaatc ttgatgtcat taggtatttc agtaccggtt gcactgttct
    28561 catttatggg ctcgattatg gctggaatct ttgctaatat cgtcaatttc aaacaatatc
    28621 aaacgattta tgctggtttc aatccagcgg ctgaaagcta tacttataat gactatttcc
    28681 aaattggtat gattgggatg attgtaagct tagttgtggt actaacggtc gctaatatat
    28741 caatgaacaa aaagaaacgc tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg
    28801 caccaatgat ctcatggtta gccgtattat taccagtttt aggggtcgtt cttttagatc
    28861 ttccaatcat cttaggtttt attctagcag gtatctgggc attgctgttt actggaaaat
    28921 tgcgcggagg ttataaagaa atctgtcgtc agtttgcgaa attatttaca gatggcgcag
    28981 tagatgttgc accaatggtt ggtttcttga tgaccttagc gatgttcaat aattcagctg
    29041 cgtatgcttc tccttatttt tcagctattt ttggagattg gataccgcaa tcaccattag
    29101 tcttggcaat cgtatttgct atattgacac cactaggatt tttccgcggt ccaatgaatc
    29161 tagtcggttc aggctcagct attttagctg tcgtattagc tgttaatcca acgatgtcgc
    29221 cagccttttt gttcccgtta tttgcaatca caacgattgc tcctcagcat ttagatatta
    29281 cacaatcttg ggttgcttgg ggattaggtt atacaaaagt aacttctcga gaatatatga
    29341 agaaatccat cccaactggt tggatcatcg gagcgatctt atgcctgatc acgttcttat
    29401 tgtacgggaa cgcctagtaa acaaaacgtc gataaaaacg attagatgaa tggaggaaaa
    29461 gaaaatgaaa cgttttgtgc gaatgggaat tgatgttggc ggtacacata cgaaagcagt
    29521 cgcaatcgat aatgcgacgc acgaaatcat cggaaaatct tcagtgaaaa caacccacga
    29581 tgatgtccgt ggagtagccg caggtgtcgt tcagtctttt caaaattgtt tgcgggaaaa
    29641 taatatcagt ccagaagatg ttgtgtttgt tgcacattct accacacaag cgactaatgc
    29701 actgattgaa ggagacgtag caaaagtcgg cgtaatcggg atggcaaaag gtggattaga
    29761 aggtttttta gcaaaacgac aaactagatt gaacgatatc gatttaggga ataagaaaaa
    29821 aatagagatt gtcaatgcat tcttacctgt caaacactta aatgtagacc gtgtttcaga
    29881 aacgatttct tctttagaaa gagaaagagc ggaggtcttg gtttcttcta tggcatttgg
    29941 agtagataat ggggaaccag aacgagttgt ttatgaagca gcatcggtaa aatcgatccc
    30001 tacaacgatg gcttctgaca tcacaaaatt atatggattg acaagaagaa ctcggacagc
    30061 agcaatcaat gcatctattt taccaaaaat gctagatacg gcaacctcta cagaagattc
    30121 cgtacgagaa gcaggggtca atgtttcttt gatgattatg cgcggagacg gcggagtaat
    30181 ggaaatcaat gaaatgaaaa aacgacctgt tttaaccatg ctttccggtc cagcagcatc
    30241 agtcatgggc tctttgatgt acttgagagc gtctaatggt gtttattttg aagtaggcgg
    30301 aacaacaacc aatatcgggg tcattaaaaa tggacgtcca gcaatcgatt attcaattgt
    30361 aggtggccac ccaacatata tctcttcatt agatgttcgg gttctaggtg tggcaggcgg
    30421 ttcgatggta cgggctaatc agtcaggaat catcgatgtc ggtccacgtt ctgcacatat
    30481 tgccggtcta gattatgcag tatttacgga gacagaaaaa atcaaaggtc ctaaggttga
    30541 atttttctct cctaaagaag gagaccctgc tgattacgtc aaggtagtga tggaagacgg
    30601 agaagaagtg actattacaa atacatgtgc agccaatgtc cttggattag tgcaagaaga
    30661 acatttttct tatggaaatg tcccatctgc acgaaaagcc atccaagcat tagctgatta
    30721 ttgtcatacg acggttgaag acatcgcaga acagatcatg gaaaaatcct atgctaagat
    30781 tgaaccggtg attttagaat tggcagacaa gtatcacttg gagaaagatc agatttctct
    30841 tgtaggtgta gggggcggcg ctgcctcatt gattacttac tttagtaata aaatgggtgt
    30901 gaaatattcg attcctgaaa acgccgaagt gatttcttct attggtgtag cacttgcaat
    30961 ggtccgagat gttgtagaac gtatcatccc ttcgccaagt aaagaagata tacgatcgtt
    31021 gaaaaatgaa gcgatgaata aagcgattga atcaggcgct acaccagaat cgatcgaagt
    31081 ccacgtagaa atcgatccac agacttcaaa agtaacggct atcgctactg gttctacgga
    31141 agtgaaagca accgatctaa ccaaggagat cacaacagaa gaagcactag agcttgctgc
    31201 agaggatatg cgattaaata aaaatgaagt ttgcttattg gaaaatacac ctttctttta
    31261 tgtatgcggc gaacagaatc gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa
    31321 aggatttatc aaggtccaac gtggacatgc ttcatgtatg aaaacaactg cagccaatta
    31381 tatgacagct gtcgaacaac tttgggaaga tatggcagtt tatcaaactg aattgattgc
    31441 ccgaccagaa ttttatctct gcttaggagc aagagtctct gattttacag cgacggatct
    31501 ggaacaacta cagcttttga tggatctgga agtatctact atggaaccgg aggaagaagt
    31561 gatcgtcgtt gccggaaata tcaaacaaac ataagttaga agagttacta aacgaattac
    31621 aacagatccc cgaagaaatt tggggattct accagtttcg gcgagattta ttttggaaaa
    31681 agattccttt gtccaagcag aaaattctta tacaacaatc aatcgattgt ggtatcgaaa
    31741 cggcatgtag catcaaaaaa aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa
    31801 tggctgttcc aatcgtttcc tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa
    31861 cttatgcaga agatgaaggg attcgattga tgacggagcc gttagaaaaa ttaaaatgtt
    31921 caggactagc ttcgatctct aaagaaacag cgcaagcttt gattattgga catgaactat
    31981 ttcaccacat cgaagcttct gttaagggaa tttatacgca aagcgaaaaa attgtgctgt
    32041 ggcggttgcc tttttataca catcaatcaa acattcgtgc attgagcgag atagcagcga
    32101 tgagtttttc aaaagagatg aatcaaagcg atttttcgcc ttatgtatta gaggcagtct
    32161 tactttggcc atataatgaa attcgtagcc aagggatctt agaagaaata aaaaaaatag
    32221 aaaaaagatg tacggagtat gatttagatt acaaataaag agactgtgac aaaagtcgtg
    32281 tcacagtcct ttttttccga ataaacgatg ttctcctgtc aactcctcgg tcacaagtca
    32341 aaatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagatga
    32401 acggcaggct cacgacctct tggaaaacgg tgttcaaaag cttgcatctg cggtaataag
    32461 tccagccaag ttaaaaacat aagaagctat ttttacttgg ctgttcttat tacttgatgc
    32521 agggcagctt tttcacaacc tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta
    32581 gctcaaaaaa aacggcaaaa tatgaggaac tattttgccg tttctttctc taatactcag
    32641 gtcaaaacgc tgattccaaa acctcttatt tgatgggtct gtcctgaata tcttcaaaat
    32701 aatcggggta attatgaatc acgtattctt gctcctcgat aatcatatgt aaatgcatcg
    32761 tgcaaagaaa tccgacttca tcaaagttat gatcaatcat tgattgaagc attgtctgat
    32821 gttcatctaa tactcgaccc cagtctaatt ctgaaatcgt caaacgtaac catctgaaac
    32881 ggttcaagtg cgtactataa ctttctagcc aatcccaaat ttctcgtttt ccagcaattt
    32941 cataacaagt tcggtgaaaa tcagtatcca aatcaaaaaa agctttttta ttttgagcga
    33001 ttactgcctg atcggttttt ccaacttttc ccgcgtataa cgggctaatg aagcagatcg
    33061 gatatcgatt tttgaaacat aagtgccgga ctgtggaatg gtttttacaa gctcttgatt
    33121 gcgcagttgg atcagtgctt ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa
    33181 cactttatcg gagattttac ttccaggtgc taactcggaa taaatgattt gtttacgtat
    33241 agattcataa gcttgctgtt gcaaattatt gataaccatt taaccttcct ttctatgcag
    33301 atataatcta ttataacata aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc
    33361 aaaaaaagcc ctgataggca tgcctattag agcctcgaaa agaaaaatat tgaatcaatc
    33421 tggtcgtacc tcttgtatcg gtttgacacg accgccgatt cttttagcaa aagcttcgga
    33481 ttcttttttg tctttgaaaa tgagcagatt gtctaagttt ttttcccgag tgcgacagcc
    33541 agttttatcg atgtaaccgt gcattacttt aacgacatac atggggatca cctgcttttt
    33601 tcttttcatt atacgaacta ctcgacacaa tacaagaaaa atgcttagac ggtctttatt
    33661 aagaatttac atcatccaaa gaagcgtacg cttggaataa ctgatctaac tccggattga
    33721 ctttgctcag cttcactaat cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc
    33781 cagtagtttt taattttttg tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg
    33841 tccccgtata cttttggata gttggatgga tgctcacttt ttctccgtaa tggatcattt
    33901 ctttatagct gcaatttact tccagaacgg ggatgatgat cccagattct tcgatttttt
    33961 tatagggaaa ccctagatgt accaataagg ctacccgagc ctcttcaaac cagcggatat
    34021 aattgctgtg atgaataatc cccatttgat ctgtttcata ataaaaaggt tctcgcacat
    34081 aacctgtaaa tttctccatg actcatgcct cgttttccat tgatttttta caaaaataat
    34141 tcatccattt cggaagtgct tcgctgattt gtgtcggcaa aacaatatat tgttcttttg
    34201 ccagctggtc tccaatgaaa ctatgaagat aaatagctgc tgcaatcgtt tggtcatttt
    34261 gaggaaactg ggctaaaaat cctgtgatca tcccagctaa agtgtcaccc atgccgcccg
    34321 ttgccatagc agcagaacct aaaggattgg tgtagaagtg ctggtgacca taaatttttg
    34381 tctcatgact tttcaaaacg agcacagcac cgatttcctg ttgcttcttt ttgttttctt
    34441 tatcccgttg ttcttgtatc ggtagagaac tcaaccgttg ccactccatt tgatgaggtg
    34501 taagtacagt ttgttcggga tagggtagca aaagatgatt agcagcaagt aaacttaaag
    34561 cagagccatc aatcaccagc cattgttctt ttgattggtt ttctagtact tgtttgaaac
    34621 gttccaaact cttagaagaa gtacccaatc cgggaccaat caagagtaca tctgcttgct
    34681 ttattacgga aagaaactgg tcagtatccg tccaaactac aatcattgct tctggcagat
    34741 gactgtgaag agcactccga tttttttcat cagttactac agtcgtgaga cccgctccgc
    34801 tgtgtacggc agctaaagca ctcataatga ttgctccgcc atattgttca ttgccgccaa
    34861 tcaaaacgat acgtccaaat gttcctttat gtgaaatttt tggacgaggt gtgatgactc
    34921 ttttcactag ttgttcgttt atcagcatct aaaaacctcc ttttactcat tatagcgtat
    34981 atttttttta gagaaaaaca gataagaaaa ggaattgtca gattttttga actatttttt
    35041 tctgtaattt gtggtatcat gaacacgaag acttaaataa gtggaggatt aactttatga
    35101 caattgattg gcaaaaagaa gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa
    35161 atcttttacg tgtcaacagc gagcgcgatg actcaaaagt aacaccagat gcaccatttg
    35221 gacctggacc tcgcgacgca ttgaaacata tgttagctta tggcgaacgt gatggattta
    35281 aagtaaaaaa tgtcgataac tatgcagggc atattgatct tggcgaagga gacgaaacgc
    35341 tagggatatt tggacacatg gatgtcgttc ctgcaggaga tggctgggac acagatcctt
    35401 atgaaccagt aatcaaggat ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc
    35461 caagtatggc agcttactat gcgatgaaga tcattaaaga acttgacttg aaactatcaa
    35521 aaaaagttcg cttcgtagtc ggcagcgatg aagaaagcgg ctggggcgat atggcttatt
    35581 atttcgaaca tgaagaagaa ccagattttg gtttctcacc agatgctgaa ttcccaatca
    35641 tcaatggtga aaaaggaaat gtttctttag ctttacgctt caaaggcgac aatgcaggag
    35701 attatgtctt gaaatctttt gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa
    35761 ctgcagcact tcaagtacca agtgcagatg cagctatcgc gatggaagaa gcattttacc
    35821 aattcatcga agcaaatcca gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa
    35881 tcgaattagt tggtaaaggc gcacatggtg caagcccaca atctggtatc aatgcaggtt
    35941 ctttcttggc attgttctta gataactatg aattcctagg atctgctaaa caatttatcc
    36001 atgtagcagc tgcttatgtc catgaagatt tttatggaga aaaactagga gtcgcttatg
    36061 aagatgaaaa aatgggcaaa ttgacaatga atgctggact atttgctttt gaagaaaatg
    36121 gtacagagga agcgaacttc atcaatatga atttccgttt tccaaaaggt gtaacagtag
    36181 acggattgca aagtgacatc gaacaaacag tggggcaaga aggcgcaaca gttactcgag
    36241 gtgctcgcgt aatggaacca cattacgtac caatggatga tccattggtg gctacattat
    36301 tgcaagtcta tgaagaccac actggagaaa aaggctacga acaaatcatc ggtggcggaa
    36361 cttacggacg tttgttgaaa cgtggcgttg cttatggagc gatgttccca ggatatactg
    36421 atacaatgca tcaagcaaac gaatttatga gtcttgatga cttgttcaga gcaacagcaa
    36481 tctatgcaga tgccatctat cgtttggcga aataattaat taaaaagagg atcaagaatt
    36541 gcaaattatt tttgcagtct tgatcctttt tttactttct actgtggtat tcatgataaa
    36601 catgatatag attcaattca tattctttct tagcgttcgt gacgactgta tccaaaatca
    36661 accctgttac taatgaaaga agtgctaaag tcatcaatcc agtacttaaa atcgcagaag
    36721 ggatgcgtgt aataaagctt gtcagcatat attcgcgaat cacaggaatc cccgtgacca
    36781 atccaaataa aaagaagaag atggtccaaa tcccaaagaa aagcaaaggc ttataatctt
    36841 taaacatttt aataatcatc atgatcactt tgaatccgtc agagaatgta ttcaattttg
    36901 attcgctgcc ttcaggacga tcacgataat cgattgggat ttcaacgaac ttgaattttt
    36961 tatctaaagc atggatcgtc aattccgttt cgatttgaaa accagaactc ataataggaa
    37021 aacttttgac aaagatacga ttgaatcctc gatagccggt catgacatca agaatttttg
    37081 ttttatacat tctagtaatt gtatttctaa caaggttatt tccaaaatcg tggaaaggtc
    37141 tcttattctc atcaaagtag gttccatttg ataggcgatc accaatgacc atatctgctt
    37201 caccagaacg taatttctcg agcaaaccat gtacagcttc tgcaggataa gtatcatcgc
    37261 catctaccat taaataatag tcggcatcga tatcaaagaa catttggcgg atcacatttc
    37321 cttttccttg acgaggttct ttttttacga ttgctccacc ttgaacggcc aattcgtatg
    37381 ttcgatcggt cgaattatta tcatagacat aaatatctgc ttctggtaat tctcgtttaa
    37441 agtctgcaat gactgttgaa attgttgctt cctcattgta acaaggaatc agaacagcaa
    37501 ttttttcttg ttgttccatt tttcactctc cttaactata gaaaacaaaa gctaaattaa
    37561 aaatacctca taatcaaagg aaatgctagt ttttcccgct aaatatcatt tatttaaaaa
    37621 gaaaatactg gaatatatgt ttggaaagct ctatctaaaa aaccacccac tacaaaagaa
    37681 ggacatgata cctctctttt gaatagtggg tgcgtattcg ttctctcact tttttgacgt
    37741 attaatctgt tgcacctttt tttcttgaaa tttctaatac ggttcctgta tgagcatctg
    37801 ctaaaaattc ataataaaca gtttcgccat cttctattcg agaaagacct ccccgataag
    37861 cactcgaccg aacggcgaat ttcctaatag gttccttttc aaaagagatc caagaacctt
    37921 cgattgcacc ttcttttaga aaagatgact tgatattttc aagtacttgg tctgctgaca
    37981 atactttttt gcgatgatac caaatcgttg aagctacccc gctcaataat cctagaccga
    38041 ttccaactgc taacccgcct ttaaaatagc ttagttcgct ttcttcatac atgtggacat
    38101 acctccttct tctcacttgt tactccttgt gcttatttta ccacactctc atattttctt
    38161 tgaaataaat aaccgctacc agaaaaaata agctataata aatacgatcg gtctgcagaa
    38221 aaaatcatat tttatccgca gatcaatata agtctgagag gagtttagtc tgaaatttag
    38281 acgaatagca tatggaagaa aaaacatttc aacgaatcaa agagttaaca gaattacaag
    38341 gaacaagcgg ttttgaacaa gatgttcgcg cttatatgga aagtcatatg gaaccgctgg
    38401 tagatgagtt acagttagat ggtttgggcg gcatttttgg tctgcgccat catgaagaag
    38461 cggatgcacc ccgtgtaatg gtcgcagcac atatggatga agtcggattc atgctgacac
    38521 aaatccaaga aaacgggctt tttaaagtcg tacctctagg cggctggaat ccttacgttg
    38581 tttctgctca gcgttttaca cttaaaacct ctactggtaa gaattatcca tgtatttctt
    38641 cttccgttcc cccacattta ttgcgaggaa caagcggaca aaaatcagta gaagtaaccg
    38701 atattttgtt cgatgctggg tttgaatcaa gagaagaggc aatgagttac ggtgtccttc
    38761 caggagacac aatcgttcct tacgctgaaa cgatcaaaac agccaacgga aagaatatta
    38821 tcagtaaatc atgggacaac cgctatggct gtacaatggt attagaagct ttagaagcgt
    38881 tgcaaaatga aacattaggt catacattga ttgcaggcgc gaatgtccag gaagaagtag
    38941 gcttgcgtgg ttctaaagct tctgtcaata aattcaagcc agatctgttc tttgcagtag
    39001 actgctctgc agcagatgat acagtgacga aaaatggcac attcggacat ttaggagaag
    39061 gtacattgat gcgtatccaa gatccaggtt tgatcatgct tcctcgtttg cgcgagtact
    39121 tgctggatat cgctgaaaca aacaatatcc cttatcaata ctttgtttca aaaggcggta
    39181 cagatgcagg tgctgctcat acacaaaacg aaggaatccc aagtacagtg attggcgtag
    39241 tgggacgtta tatccatacg catcaaacga tgttcagtat tcgcgatttt gaagctgcac
    39301 gagaaatgtt gattcaaaca ctgaaaggtt tggataaatc aacagtgaat acgatcgtat
    39361 acggaaaata atgcgatagt gaaactataa aatctggcaa aagattgctt tttgccagat
    39421 tttttttata aaaagaggat caatgtttgt tagttcgaaa gaaaggtgta taatttaatt
    39481 gaaaaataca taaacagaaa gtagaggaca aagaatgatt atcccaaata gtttagaaga
    39541 attagcaggc tacgtagaaa ctggaaagag tgtatttttc tttactgccg gctggtgcgg
    39601 agactgccgt tttatcaaac cgcaaatgcc tgaaattgaa aatgactttt ctgattggca
    39661 atttattgaa gtagaccgtg acaagtacat agatgtagcc gctgaatgga acatttttgg
    39721 tatcccaagt tttgtcgtga tccaagacgg aaaagaatta ggtcgtctag tgaataaaga
    39781 tcgtaaaaca aaacaggaaa tcgaatcgtt cctagaaagt gtaacaaaag gctgacatct
    39841 gttaggaggg gtcaagatga atcaagaata taagaatatt ttagtaggta tcgacggtag
    39901 tgaacaagca cttgctgctt tcaaaaaagc ggtagaagtg gctcgccgta ataatgggaa
    39961 agtatatgta gcaaacgtaa tcgatcagca attttataac tttatgggct atgcacctat
    40021 tgatcaaaat ctagtcgatc agcaaactga aacagccaag gaaatgatcg aagaatgtaa
    40081 agaatatggg aaatctgttg attacacaaa tatagaagga attattgcat atggttctgt
    40141 tcgtgaatcc atggcagtca aattgccaaa aaaatatgag atcgatctaa tcatggttgg
    40201 gcagtcaggt ctaaatgcag tcgaacgctt tatgacagga agtgtagcca gctacgtgat
    40261 acgtcattca ccttgtgatg tcttgatcgt ttctgacgaa gaagaacagc caagtaatta
    40321 agcaagtgga acttgtacga cctttttaat caattaacta aaacagtaaa gggactgtga
    40381 caaaagtctt gtcacagtcc cttgttacga ataaatggtg ttccagaaga ggcttcttcg
    40441 gaaataaagc atgaaaaaaa tttcatgcag tgaatgcaat tttttgttaa gataaatctc
    40501 agcaaaggat aatggaggaa agaacatgat ttttgcttat aacaaagcac acgtcggaga
    40561 caccttgatg gttattatag ccgacgataa aggaacagaa aacactgttg aacgcaaatg
    40621 gaatgttgct cggatcaaag atgaacaagg tcaagttgtt gcttggaact ttttccatat
    40681 ctccgatcat ctaacaatcg aagggaacgg ccaagtaagc ttaacagatg aggaattagc
    40741 gaaattgaat cgattgatca aagaagcagg attcgaagaa cagcttgaag ctgatcatga
    40801 accgaagatc gttgtcgggt acgtaaaaac atgcgtccca catcctgatt ctgaccattt
    40861 atcgattact gagacagaag tagacaatgg tacagtattg caaatcgttt gcggtgcacc
    40921 caatatcgaa gctggacaaa aagttgtcgt tgcaaaacca ggagctatga tgccagatgg
    40981 aatgatgatc tggccaggaa ctttgcgagg ggtagatagc tatggaatga tctgttccgc
    41041 tcgtgaattg catttgccaa atgctccagc aaaaaaagga atcttagtat tgcctgagga
    41101 tgccgtagtc ggagaagcat ttccaaaata aagaggttgt gacaagtctt tgtcacaacc
    41161 tcttttttat taattttcac tattttgatt ttgttttaat gccgattgat cgatcgtcaa
    41221 ttcaacagta gttgtttttt ctttcgagcc ttcatagtaa gtgattttga cagaatctcc
    41281 cacttttttc ttatacaaag cagattgaag ttctacacca gaagaaactt ctttgtcatc
    41341 gatcttagta atgacgtcgt attgtttcaa tccagctttt tcagccggtg tagctgtttg
    41401 gacatttgtc acgatgacac cgttagttac agatgaagga atcttcaaga tttgttcttg
    41461 ttgctgagta gaaacggcag ataagtcgac cattgtgata cctagagctg gacgtgtcac
    41521 ttttccgtct ttttccagtt gattgatgat attcactaca tcgttacttg gaatagcaaa
    41581 tcccattcct tctacgctta cattagaagt agattcagaa gtacttgcga tcttacttga
    41641 gttgataccg ataacttgtc cttcgatatt aactaaagga ccaccagaat ttccagggtt
    41701 gattgctgca tctgtttgga tcgcattgat attgactgtt tcgttcgatt cattcgtact
    41761 tgtcacttgt cggttcaagg aagaaatgat tcctgaagtg acagaatttg catattcaga
    41821 tcctagtggg gaaccaatcg caatcgcagg ctcaccaacc ttcagagcac ttgagtcacc
    41881 aaaagaggca actgtttcga ctttgtctga attgatcttc aacaccgcta aatctgaata
    41941 agcatctgtt ccaactaatt cagcttttac cttcgtaccg tctttcatca agacttcaag
    42001 accttgctgg ccatctacta cgtgattatt cgtgactaca taagcagtat tcccgtcttt
    42061 tttgtagatc acgccgcttc cttcactata agcttccaaa ttactatcat ctgagctgct
    42121 actttcttct tgctgcccga atagtccgcc aaaaccgctt gattggtttt gactttgtaa
    42181 gttgataaca gaaacgacag cgtcttgaac tttatctacc gctttggtga tatcagaatc
    42241 tacattgact ttgacatttt ccacaactgt ttcaccggca gaattctgat tgccgcttgt
    42301 agcagtagaa ctattgttgc cagtgcccat cgctgcataa aaaatgccag ctgtcagtag
    42361 tcctccaacg atccctccaa caagccccag acctaactta cgccagaggc tattattttt
    42421 actttttttc atctttggtg tcacattttt tctatccatg tgttcctcct ccaattacat
    42481 tcatttatct actgttagta tagtcttatt tgttgacttt agtctacttt gagactgtga
    42541 actttttatg aaaaacttta ggaaaaatca ttgttttcag atgaaaatat ttgattttct
    42601 ttttccacag atactgtgta aaactacgcg gataggattg gaaaaataag ggataaaaca
    42661 tatccacagc ctgtgtataa agtggataaa ttcttataaa acgtatgttt gcctaatgaa
    42721 tgtgatgaaa taaggtaaaa taacactgtt ttctgtggat aaacctgtgg atgttgtgca
    42781 taactgttgg ggaaatcttt ttaacaaaaa tagccaaaat aaacatatag acgaaaaaat
    42841 tgattataat aaacaaagtg agcatgaaga ccaaaggaga gaaaacatga atatcaaaat
    42901 tatctcagta ggaaaattaa aagaaaaata cctgatccaa ggtatcaatg agtatgtcaa
    42961 acggctgaat gcctatgcaa aaatcgaatt gatcgaagtt cctgacgaaa aagccccgga
    43021 gaatttaagc gaagcgcaaa tgagacaagt aaaagaaaaa gaaggggaac ggatcttagc
    43081 gaaaatcaaa gaacaagaat acgtctatgc cttagcaatt gaagggaaaa atcctacaag
    43141 tgaatctttt gctaaacaaa tcgaccaatt aggtatccaa ggaaaaagcc accttgtttt
    43201 tgttatcggc ggctctttag gactcagcga agccgtcatg aaaagaagca atgctcagat
    43261 ctcttttgga aaaatgacgt atccacatca attgatgcgt ctgatccttg tcgagcaaat
    43321 ctaccgtgcc ttccggatca atgcgggggc tccgtatcat aaataatgat attta
//
LOCUS       DAZUJO010000016        41513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000016 DAZUJO010000000
VERSION     DAZUJO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.016"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..756)
                     /locus_tag="ACG8OL_000954"
     CDS             complement(<1..756)
                     /locus_tag="ACG8OL_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4816958.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
     gene            complement(1014..2291)
                     /locus_tag="ACG8OL_000955"
     CDS             complement(1014..2291)
                     /locus_tag="ACG8OL_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816959.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(2370..3014)
                     /gene="pcp"
                     /locus_tag="ACG8OL_000956"
     CDS             complement(2370..3014)
                     /gene="pcp"
                     /locus_tag="ACG8OL_000956"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS4816960.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(3213..4157)
                     /locus_tag="ACG8OL_000957"
     CDS             complement(3213..4157)
                     /locus_tag="ACG8OL_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS4816961.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(4159..4845)
                     /locus_tag="ACG8OL_000958"
     CDS             complement(4159..4845)
                     /locus_tag="ACG8OL_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS4816962.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            5319..5639
                     /locus_tag="ACG8OL_000959"
     CDS             5319..5639
                     /locus_tag="ACG8OL_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS4816963.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            5793..6698
                     /locus_tag="ACG8OL_000960"
     CDS             5793..6698
                     /locus_tag="ACG8OL_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4816964.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            6695..7468
                     /locus_tag="ACG8OL_000961"
     CDS             6695..7468
                     /locus_tag="ACG8OL_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4816965.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7649..9073)
                     /locus_tag="ACG8OL_000962"
     CDS             complement(7649..9073)
                     /locus_tag="ACG8OL_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS4816966.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            9284..9730
                     /gene="nrdI"
                     /locus_tag="ACG8OL_000963"
     CDS             9284..9730
                     /gene="nrdI"
                     /locus_tag="ACG8OL_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4816967.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(9795..11777)
                     /locus_tag="ACG8OL_000964"
     CDS             complement(9795..11777)
                     /locus_tag="ACG8OL_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4816968.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11791..12687)
                     /gene="murQ"
                     /locus_tag="ACG8OL_000965"
     CDS             complement(11791..12687)
                     /gene="murQ"
                     /locus_tag="ACG8OL_000965"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4816969.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(12690..13751)
                     /locus_tag="ACG8OL_000966"
     CDS             complement(12690..13751)
                     /locus_tag="ACG8OL_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4816970.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(13962..14489)
                     /locus_tag="ACG8OL_000967"
     CDS             complement(13962..14489)
                     /locus_tag="ACG8OL_000967"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4816971.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(14556..16058)
                     /gene="eat(A)"
                     /locus_tag="ACG8OL_000968"
     CDS             complement(14556..16058)
                     /gene="eat(A)"
                     /locus_tag="ACG8OL_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4816972.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(16316..17437)
                     /locus_tag="ACG8OL_000969"
     CDS             complement(16316..17437)
                     /locus_tag="ACG8OL_000969"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4816973.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            17563..18504
                     /locus_tag="ACG8OL_000970"
     CDS             17563..18504
                     /locus_tag="ACG8OL_000970"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4816974.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(18654..19550)
                     /locus_tag="ACG8OL_000971"
     CDS             complement(18654..19550)
                     /locus_tag="ACG8OL_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS4816975.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            19819..21159
                     /locus_tag="ACG8OL_000972"
     CDS             19819..21159
                     /locus_tag="ACG8OL_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4816976.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            21377..21820
                     /locus_tag="ACG8OL_000973"
     CDS             21377..21820
                     /locus_tag="ACG8OL_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS4816977.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(22324..23742)
                     /gene="nhaC"
                     /locus_tag="ACG8OL_000974"
     CDS             complement(22324..23742)
                     /gene="nhaC"
                     /locus_tag="ACG8OL_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4816978.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(24145..25368)
                     /locus_tag="ACG8OL_000975"
     CDS             complement(24145..25368)
                     /locus_tag="ACG8OL_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4816979.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(25399..26238)
                     /locus_tag="ACG8OL_000976"
     CDS             complement(25399..26238)
                     /locus_tag="ACG8OL_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4816980.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(26255..26935)
                     /locus_tag="ACG8OL_000977"
     CDS             complement(26255..26935)
                     /locus_tag="ACG8OL_000977"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS4816981.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(26937..28010)
                     /locus_tag="ACG8OL_000978"
     CDS             complement(26937..28010)
                     /locus_tag="ACG8OL_000978"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS4816982.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(28066..28304)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(28347..28580)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28657..29328)
                     /locus_tag="ACG8OL_000979"
     CDS             complement(28657..29328)
                     /locus_tag="ACG8OL_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4816983.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(29415..32063)
                     /gene="mgtA"
                     /locus_tag="ACG8OL_000980"
     CDS             complement(29415..32063)
                     /gene="mgtA"
                     /locus_tag="ACG8OL_000980"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS4816984.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(32405..32579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            32752..33189
                     /gene="ndk"
                     /locus_tag="ACG8OL_000981"
     CDS             32752..33189
                     /gene="ndk"
                     /locus_tag="ACG8OL_000981"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS4816985.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(33211..34077)
                     /locus_tag="ACG8OL_000982"
     CDS             complement(33211..34077)
                     /locus_tag="ACG8OL_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4816986.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(34237..36330)
                     /locus_tag="ACG8OL_000983"
     CDS             complement(34237..36330)
                     /locus_tag="ACG8OL_000983"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4816987.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(37079..37702)
                     /locus_tag="ACG8OL_000984"
     CDS             complement(37079..37702)
                     /locus_tag="ACG8OL_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816988.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(37861..39258)
                     /locus_tag="ACG8OL_000985"
     CDS             complement(37861..39258)
                     /locus_tag="ACG8OL_000985"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4816989.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(39290..40261)
                     /locus_tag="ACG8OL_000986"
     CDS             complement(39290..40261)
                     /locus_tag="ACG8OL_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4816990.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            40635..41060
                     /locus_tag="ACG8OL_000987"
     CDS             40635..41060
                     /locus_tag="ACG8OL_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4816991.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        11982 a         8667 c         7292 g        13572 t
ORIGIN      
        1 tttttgttcg agtaaatctt ggagaagttc ggcgtaatag atcgtttttt gacagtggat
       61 actgtctttt tcaacagctg gataggcaca ctgtgcatag tggatgattt gatctgcctt
      121 ttgctcggca cgattcgcgg agattttttt ccgtgtttcg ttgatcagtt tattttttat
      181 ttttgtccga gtagatgcca atacaaaatc tggatgggga aaaagacgga tcaatgtcaa
      241 tgcgtaaggg gtcagcctgt ttgaaaagaa ctgttcaagc tctggaaaag tcaattgaag
      301 gcagttgtgt aaatccatgc gcagacgagt gattttcttc tcaatttctt gataaaaacg
      361 ggctaaatcg cgcatctcta agtaaaaatt gctttgtact tcttttggct gacgttgatt
      421 tttaagatga gtctgtgcga gccggtgggc atccgattta tctgttttcc aactgcgtaa
      481 ggtgctttct tccatctgtt ttttcgcctc taacgggttt aacaaacagt aagaaaagtg
      541 atgatcttga aagaactttt cgactggtct ggaataaatc cccgttgctt caaaaacaat
      601 caatggacgc tcaggcaagt gctggatctc ttcgagtaat gcatgaaatc ctctttgagt
      661 gtgttcaatc tcgtcttcaa ataagcaggt gtcgtctttg tataggacac tgtagctgtg
      721 tcccattgaa acatctaatg caagaatgta aggcatttgt tttcctcctt tttaagtttt
      781 gaaagaactt tactgcttca tttcgattcc aatttcctat acacgggctc atggccccaa
      841 catacgtaaa cctgattcta aaatgacagt aaagaagttc ggtttttgat agggactcgt
      901 agtcctcaag gctttgttcg aactttcttt cactcttcac tataatctaa aaatagaaaa
      961 tagaggagaa catcattcgt caatgatgtt cccctctaat cttagtatgt tttttatagt
     1021 aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc taacttctga
     1081 taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc tttccatttg
     1141 tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg ccatttttct
     1201 tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc tttacgcaat
     1261 acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc ttctgtaaaa
     1321 ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat gaatcgttgg
     1381 tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg ttttgtcttt
     1441 ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac ttcttcatat
     1501 acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa ttcttctaca
     1561 tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg tcgctttttt
     1621 gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc ttgataatcc
     1681 gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc taattttata
     1741 tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca gtatttttgc
     1801 catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc gtctaaggcc
     1861 agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga gacatgcagg
     1921 tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg cgatttaacg
     1981 atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg gcttcgtttt
     2041 gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc agctaaataa
     2101 tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac tagttcccgc
     2161 tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta ttgtttagaa
     2221 attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa gacggattgc
     2281 cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac taacataacg
     2341 ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt tccggaaata
     2401 tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc aatgtcttct
     2461 aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga gaatggcacg
     2521 tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag caaacgatac
     2581 atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg tagaccatga
     2641 tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc ggctggaccg
     2701 ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat tcttgcttct
     2761 gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc ttgtccgaca
     2821 cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg actgcttttt
     2881 tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc atctggcatt
     2941 ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc aaaacctgtt
     3001 actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt tatcttttat
     3061 cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata acataacaga
     3121 tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag aagaggttgc
     3181 gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat ataagcaatt
     3241 tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc gtatttgtct
     3301 ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg tgtcagcagg
     3361 gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt cgctccatga
     3421 ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc aaatgcattc
     3481 cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc gatgttcaca
     3541 tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac tccagcgctt
     3601 gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg ccctactgtg
     3661 cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg agaaaagaac
     3721 atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc acctaaattg
     3781 gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat aaaaattttc
     3841 attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg gctttctcct
     3901 ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc accgttcacg
     3961 attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca gaaaaaggct
     4021 gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt gtaaatcaat
     4081 acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa aatatcagga
     4141 tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg ctaatttctt
     4201 ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca ctggaacttc
     4261 cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg ttgattggac
     4321 tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc agacattttc
     4381 catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt ctaacccttt
     4441 tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc cagcaactcc
     4501 accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa tatctacaat
     4561 cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt ctttcagacc
     4621 atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca tcccgcgatt
     4681 gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac cgccagtcaa
     4741 agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa atgcaaaccc
     4801 gagaataaca atcaagacgc ctaaaaggct aagataattt tccatatatt ttcctccata
     4861 aaatgacaat atttttttaa ttataaagca aaacacataa aaaatatagt tgttttctca
     4921 cgaaatagac actattttta aatatttttc gttatttttt tatcacaaaa attatcttac
     4981 ttattgtaag gtaaagcttt ttttaattta ttgcccgata atatatagaa ataaggtcta
     5041 gttactgttt tttttaaatg attaataaat aacaacttct ttctatgaaa ttgcaaagca
     5101 ttcatcttct tttccgctaa cgatatatcc gtcagtgctt ggtacacttt actaaaaagt
     5161 atgagattgc cgatatctgg ataggtgtgc tatactacag aaaaagatat acaatctgcc
     5221 accgggtagg ataaagagga tgatcattcc attaggtctt tgaagggatg attttcctct
     5281 ttttgtgtat ttttttatag ttaaggagga atgtaagtat ggcatggatg gaattaatca
     5341 tagcaggaat tttggaagta ttctggtcaa cgatgatgaa atggagtgat ggtttttcta
     5401 aaatcaatta ttctttttac acagtgattg gcatgattgc tagtttttac tttttgtcaa
     5461 aggcaatcaa aagtttgccg atgagtctct cttatcctat ctggacaggg attggcgcag
     5521 ttggttcggt cataattggt gtggtcttgt ttcatgataa gctgaatctt tccacttggt
     5581 tctttgtggg gatgctactc atcagtatca ttggcatcaa gattactagc ggccactaga
     5641 cacttggaat aagtactgat cagaaaaaat aagccttttt atttaatatt caattttttt
     5701 atgaaaaaat aagaaataag tccaatgacc tagcgacttc tccactcatc aggtgtatgc
     5761 ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc caagaattat
     5821 ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta tcccctggca
     5881 aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg aaagctattt
     5941 taggactgat ccattactca gatggaacaa tctgtattgg tgaaaatgag atttctccct
     6001 ctagtcataa agggttggaa caagtaggag cattgatcga atacccgggt atctatccat
     6061 tcttaacagg ctatgatcat ctgaagttat tctctgaaac caacgatgta tctgctatcg
     6121 acactatcgt gaatcagctc aagatggaaa agtacattca caaaaaagcg aaatcctact
     6181 cgttgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat ccgtctatcg
     6241 ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat gtgcgaaaaa
     6301 tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac ttactcagcg
     6361 agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaagatc atcaaacaat
     6421 gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct acaacaaatg
     6481 atctagctgc tcgaacgatc ttgacagaag ctggatatac tttggaagat cctgataaaa
     6541 tcaagctttt aaaagaaaac gaagaattat taggaaccgt gatccagcta ttgtctgcac
     6601 aaaatatcca tgtgacagac gtccagcatc tgacgaatga tttggaatct tctctattgg
     6661 aaattctgga ttcagtaaag ggggaagaag aaaaatgaca ttgcttgtaa aacaagaatt
     6721 atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag ttgttttgct
     6781 cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag ttgaaatgac
     6841 tgctcagctt tttagtgcca catcttggat tgtctttatc atgattgcag ctgcaagcac
     6901 gatcatttcg atggaagcac aatatggtac attgaaaaat ctcctttatc gaaagtattc
     6961 tcgaggagaa atattagtaa gcaaatggat cacattagtt atttattctg tttatttata
     7021 ccttttagct attattgtca ctgtgttgat gaaactgatt cttttccctt cgatttcctt
     7081 cacggagaag gtttctactg gtcaaacact gatccaaagc ttgttcactt atactttggg
     7141 tagttatatc ggtttatggc tgattttaag tttagtgttg atgattgctt gctttatcaa
     7201 tagttccggc gcatccatct cagcaggaat cgtcttttat tttgcttcgt caatcatatc
     7261 tggtatcctt attgcactca tccaaaaatg ggagtggatc aaatggaatc cgatcagtat
     7321 gttgaatctg caaaaccaaa ttggaaacga agagatcatg aagcaattga ctcatctttc
     7381 tactaatcaa atgctattcg gtaatttagc ttacatcgtt ttgttcttag cattaggata
     7441 tctcgtattt aagcgaaaaa atgtatgatg actcatcaaa aaagaggctg tgaaagagac
     7501 gttcagcctt attgccgatt ggctgtctct taaacaccgt ttattctgaa aaaggagctg
     7561 tgacaacatt tgtgtcacag ctcctttcct agtatgtttt tgaataaacg acaggctcac
     7621 aacctctctt tatacttctt ttatacttct atacgtgaag catcccagtg gcaaataaaa
     7681 ccattgcaat cactccgacc atcgtcacac ctataagtaa aatcttatct aagtggatat
     7741 gttgtacagg ctcgttttgc tccttacgtg ctttgaggta aaaataaatg cctggcaaga
     7801 aaagaacaag aacaagcatt acttcattaa aggctacgac tagcatagcc catacttgaa
     7861 aggcacttgc taaaagccct actaaaaatt ggacccattc tttccgctga taagaaagtt
     7921 tcatttggta caatcctaag aaaagccacg tgaagaaaat agctgctgta ccaagtgtgt
     7981 aagcaaagtt ataagcactg tcagtgaata ataatgaaag taaaaagaca ttcgtacata
     8041 acgtcgtcaa tacgatagca taagttggtg ccccattttt gttgagtttt ccccatgctt
     8101 taggcaaaag tccgtcttca gccatgtttc ccattgtttc aatcggtaag atcgtccatg
     8161 agagccaaac accaactaaa gaaatgatca aaccgccgtt gatcacgact gctccccatt
     8221 taccaaccat catttcaaac acatagccca tagcaggctg cgccttgatc gtttgcagct
     8281 gattcatcgt cattgtccca tatggaagca tggaaaccaa gacgtaaata ataatcaagc
     8341 cgaatatccc tagaatcgtt gctttcccaa catctgattg tttttttgct cgtgaaccta
     8401 cgacagaggc tccttcaata ccaacaaata cccaaagcat caccatgatg caactcaata
     8461 cttgactgga aaacgggatc ggctgtccat tcgcattcaa tataatgtcg ctagaaaagt
     8521 tatcaatgaa ttgttggatc gaaaacgtgc tgatagctac tacaataaat aaaaataatg
     8581 gcactaattt acagaatgtg acgatagcat tgacgaaact agcattttcg actccccgat
     8641 tcactagcca tgcatatccc cataaaaaga ctgtactcat cacgatcgat gccagatttt
     8701 gcccaccttc aaatactggg aagaaatatc ctaatgcgct catcaacaat gttgcaaaag
     8761 ctacgttgcc taaccagcat gtgagccagt aaccccaacc gctgataaaa ccagcgaaac
     8821 ggccaaaacc ttcttctgcg taactaaaga tcccgccttt taaatctgga cgttttttat
     8881 tgatattatt gatcgacaag gagagcatca acattgctgt tcctacgagt acccaactga
     8941 aaatagctgg gccgccagcc gatccggaga tgtccgtcat aataccaaat acgccacctc
     9001 caatcgctga accaattact aaggcagcaa gtggaattaa accaattcct tttttctgtt
     9061 gctctgtatc catgtaagca tcctccaaaa taaaaccgct tttatcaaaa agtataacaa
     9121 acctttttta tttttactta attaataaaa actttaatag aaatataatt ctaccggctt
     9181 ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac caaaaaactt
     9241 aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca ttttatatat
     9301 atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg ctgaaaaaat
     9361 gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg aaaatagtga
     9421 ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag acggcggaaa
     9481 tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat atctggaaca
     9541 tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa acttcaataa
     9601 tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt tcttggcaga
     9661 ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt tagcagaaaa
     9721 caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct ttcgtttcta
     9781 ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt tgctttacct
     9841 gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac gatcatggat
     9901 gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag cgtatcaggt
     9961 gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa gtcaactgtg
    10021 ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc aatagcgtgt
    10081 ttcgttggga agacagagct gactacccca gtaactggag aatagatatg tccgttttct
    10141 ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc acttgcttta
    10201 tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact ttcagctgaa
    10261 gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt cgctaattct
    10321 tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa gccaccagca
    10381 taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat caatgggatc
    10441 aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat tccgatcact
    10501 gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag aggtaatgtt
    10561 actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc tcctttgatc
    10621 atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc tgcacccact
    10681 tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt ttcatcaatc
    10741 atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat cggcaagaat
    10801 aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca gttgatccca
    10861 ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat ttcaatcaga
    10921 ccgatggcaa tcaatgaaag agttggtgta acgatgatat ctaccgcatc aggtactact
    10981 ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac acctaggatc
    11041 ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg tgcttccgga
    11101 ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg tgttgcacca
    11161 aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat cccgtttttc
    11221 aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt tccggctgtg
    11281 actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac aaatgcagga
    11341 atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt agatggtttt
    11401 tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg gtcttttcca
    11461 gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc taatttcacg
    11521 cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc gatgatgact
    11581 tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa ttcagagaca
    11641 tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg gacaatgctt
    11701 tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc ttccaaagtc
    11761 agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa atccgtttgc
    11821 ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca tgacgatagc
    11881 cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt aatcagcttc
    11941 tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg ttggtttgac
    12001 atcgaccatc aagttattga atactttacc tataccaatc atactgatag tagaaatcat
    12061 attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg tcaaaaattc
    12121 tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg cttgtttatt
    12181 gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc caccgatcac
    12241 atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg tcaaattcag
    12301 cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga cagctacagt
    12361 cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta caccgaatgt
    12421 cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg ctcccatata
    12481 aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg gttcgatact
    12541 tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca ttagttgaac
    12601 ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc gttccgttgt
    12661 taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat aaattgatag
    12721 ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac tttagctact
    12781 acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc acccatatag
    12841 cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc tgtatttctt
    12901 gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac atctcttgca
    12961 tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc atctacaggt
    13021 tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc ctgaacgttt
    13081 tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa tagatctaga
    13141 gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc ttgatatagt
    13201 gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc ttgaattttg
    13261 agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg acgtggataa
    13321 taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa ttcatcgata
    13381 tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga ccagtcggca
    13441 atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat tgctttcaaa
    13501 ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga aatatcgacc
    13561 atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg cttttgatat
    13621 gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa gccattggat
    13681 gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat aaatattgaa
    13741 aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga ctagagtata
    13801 tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca tttctaaagg
    13861 ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct aagcaaaagc
    13921 agtgccttga aatattttat ttaaataaca caaaaacata attactcata agaaaccaat
    13981 tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg aaaataagca
    14041 ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc ttcgtcttcg
    14101 atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga ctccccattt
    14161 gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt gcttggcagt
    14221 ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag cggcaagctg
    14281 agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga atggatgatt
    14341 ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc ctgcatctgt
    14401 cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc gttccaagtt
    14461 gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc aaagttcccc
    14521 ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag agaaataatc
    14581 tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat ggcaggtttc
    14641 acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata attcaatggt
    14701 tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc cacttttttc
    14761 cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc catcccaagc
    14821 tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc aaattccgcc
    14881 aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag atgttttggc
    14941 tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg gataatggat
    15001 gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc gattgtaaaa
    15061 tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac gcttaaaagt
    15121 cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc gatatctttt
    15181 aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc tttggatcgt
    15241 ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct agattcagta
    15301 tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga ccattcggct
    15361 ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc attttgagcc
    15421 atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt cccttgataa
    15481 agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc gataaatccc
    15541 cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc cgctacttgt
    15601 tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa atgagtgtca
    15661 tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag tgtgctgaat
    15721 tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc gatttcccat
    15781 atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt atcttttgtc
    15841 tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata aggtagtttg
    15901 ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat aagtcctagt
    15961 ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc ttggctgtcg
    16021 tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct cgtaaaaaaa
    16081 gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac acacaaaaag
    16141 acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt ccttgtctcc
    16201 acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat gcacggatcc
    16261 ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt gtcaactact
    16321 tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt ttttctaatg
    16381 tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata cttaagacaa
    16441 ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa atagcgatta
    16501 ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt ccgtggatcg
    16561 tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg atcttttttt
    16621 ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct cccaacaaaa
    16681 aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct aaatccacct
    16741 cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag aaagctagcg
    16801 tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt tgacctgtat
    16861 atggattggt aacatctgca gcgataatca attttacttt agaaacaaga acgtttcttc
    16921 gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac ccttctaaag
    16981 aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt cctcctaaag
    17041 tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct cctaaggcat
    17101 aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct aaccccaatg
    17161 cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat aaatagctga
    17221 tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt cccctgagcg
    17281 cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca ttgatttttc
    17341 ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat gaagaagcac
    17401 ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac tttcttctta
    17461 agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt ccatttttct
    17521 atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat cgcaatagct
    17581 ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc
    17641 ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg gttgattgct
    17701 gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc
    17761 gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca attagatgat
    17821 atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg tttactaaat
    17881 ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat cttatttggg
    17941 atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt aggtgatgga
    18001 gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa
    18061 gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta ttccatttgg
    18121 cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga ttgtaatatt
    18181 gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt atctgaattt
    18241 gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta ccaacatatt
    18301 gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta ccaagacctg
    18361 atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga
    18421 caaaaatatg atattgctac accatattgt gcctttttga cccaattagt ccatgcaaaa
    18481 gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga atcagcagct
    18541 ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct catgatgcca
    18601 taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt tttttatttg
    18661 ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc gttagtgata
    18721 aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag gattggctta
    18781 aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag caaaaaaata
    18841 ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac tagtttcttt
    18901 tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt
    18961 tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt aagatatcca
    19021 ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc tctttttata
    19081 aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc accaaaaaat
    19141 agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta
    19201 tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc atcgattgcc
    19261 ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa
    19321 aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc catatcaaat
    19381 ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc tgatttaatg
    19441 aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat ggaaggcaaa
    19501 aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat cttttccgcc
    19561 tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa
    19621 cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga aataaatcat
    19681 tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttttt
    19741 atcattagtc attgttaaat attgctattt attcttaata taaattttga ttgtgaattt
    19801 aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat
    19861 atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa
    19921 gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg gctgatttta
    19981 tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga
    20041 ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc
    20101 tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg
    20161 cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact
    20221 acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt
    20281 gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt cggtcagcaa
    20341 atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact
    20401 cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt
    20461 tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa
    20521 gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt
    20581 ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgatggta
    20641 tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat tgtccttgct
    20701 ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg
    20761 aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa
    20821 cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc
    20881 ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg
    20941 atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa
    21001 ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg
    21061 aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat
    21121 caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt
    21181 tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag tcttgttgca
    21241 actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc
    21301 tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa
    21361 aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac
    21421 ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag
    21481 tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt
    21541 gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct
    21601 tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc
    21661 ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta aacgagctga
    21721 tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga
    21781 gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa
    21841 aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta cttctggaac
    21901 gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    21961 aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
    22021 acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag agagacaaat
    22081 ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg
    22141 aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt
    22201 tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa actggctttt
    22261 gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc cctttttatg
    22321 tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttttt
    22381 tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa gcaaactgaa
    22441 aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta cgaaacttcc
    22501 ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta gcgttctacc
    22561 taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat taccaggcag
    22621 aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg tcatcaatgt
    22681 tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca tcacggttga
    22741 gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa tcaatgacac
    22801 agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt gttggtttcc
    22861 agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg aagtttgtgg
    22921 gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg ttaaaattgc
    22981 cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg accagattga
    23041 aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa gacttgcggc
    23101 tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccgggacagt
    23161 tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctag
    23221 atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg aaacaatagc
    23281 tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa atgcaatccc
    23341 aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg cacacacaac
    23401 aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga ccattagagt
    23461 tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa
    23521 gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg
    23581 gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga tcgtgatatt
    23641 aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa ccattaatac
    23701 aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct ctcctttttt
    23761 agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca attcatccgc
    23821 gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat gactccgcgg
    23881 taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc aaatgtatgg
    23941 actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga
    24001 acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta ccttccataa
    24061 gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac
    24121 tttcacacac aagattattt tttactagaa agtagcgccc tcttgttttt ttcttgctac
    24181 tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca acagatcaag
    24241 agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt
    24301 agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt
    24361 aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac cgagacttgt
    24421 tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat gctggaagaa
    24481 taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga acaatccaaa
    24541 ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca ctttttcata
    24601 tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag cagcgtaatc
    24661 tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat tgacatcttt
    24721 tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg cttccaaagc
    24781 atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc
    24841 gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg gatggacgca
    24901 taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc
    24961 gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac cagtttcatc
    25021 aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca aattatgccc
    25081 gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc
    25141 gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc
    25201 tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat aaaactcata
    25261 gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt ctggtaaacg
    25321 atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa aaaattcctc
    25381 ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca cgcttgatcc
    25441 ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt ctactatttt
    25501 ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca cataaggacg
    25561 tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa atccagcatc
    25621 tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac gtgctgtttg
    25681 ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat cagaaacagt
    25741 gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca ataacagaag
    25801 agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt ctttgatatc
    25861 agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag tatttccgat
    25921 cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt gatgttggaa
    25981 agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt aatctgtaaa
    26041 tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt tgtcccatac
    26101 atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt cagttgtatc
    26161 tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa tgactacaaa
    26221 acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt ttaatcagtt
    26281 gattactgat aaattgactt aaaaaaacta agattagaat caataatgta gccacaatcg
    26341 ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct aatccgcctg
    26401 cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga
    26461 cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata ccgattggac
    26521 tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc agtgcgttct
    26581 cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa gctgctttcg
    26641 caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa agcaagataa
    26701 tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat agcagacggt
    26761 tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg attccaatca
    26821 tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa
    26881 attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca gtcattttat
    26941 cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa aatatttatt
    27001 tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt ttctccagct
    27061 aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt gccatataaa
    27121 atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc gttcgtctga
    27181 tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc aaaagcagaa
    27241 tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat aaaatctttg
    27301 gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc aacgattctt
    27361 ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg
    27421 atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc aaggatctga
    27481 agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac cttaggttca
    27541 ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag ttgtttagga
    27601 taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac tttttgacga
    27661 cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc aaaaatagtt
    27721 cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc
    27781 aattctttgg gagagagctg actcagattc gtctgattga caatgacttc tcctgctgtt
    27841 ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc tgagtaaccg
    27901 acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac tgcctgtatc
    27961 tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat taaaattcct
    28021 ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt
    28081 ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtacgat
    28141 catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa
    28201 ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc
    28261 tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaagt
    28321 ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt
    28381 cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac
    28441 ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag
    28501 cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact
    28561 ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactgaa
    28621 aagtcaagta cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatttaa
    28681 catttcaata acagttcgaa gatcttttcc ttccgcaaga agtttcaatt tttccttttc
    28741 ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc
    28801 tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc
    28861 taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac
    28921 ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat ccaagatatc
    28981 tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa
    29041 gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg
    29101 ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc
    29161 tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc
    29221 ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt
    29281 ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct atacattaat
    29341 cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca gaagcaaaga
    29401 accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt cttcacagct
    29461 gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga
    29521 agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata
    29581 attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat aaatggtact
    29641 ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat
    29701 cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt atggaaactt
    29761 ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat
    29821 atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc attccatttt
    29881 tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca
    29941 tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac
    30001 gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata
    30061 tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc
    30121 aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct
    30181 acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa
    30241 aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga
    30301 ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt
    30361 tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact
    30421 gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc
    30481 agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc aagataacca
    30541 atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc
    30601 ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt
    30661 acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta
    30721 gaaatgttca gcatttcttc aatcgcacct tttgtgatca tttgtgtttt accagctgga
    30781 tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt
    30841 tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct
    30901 aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga
    30961 tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat
    31021 gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat ggcattcaaa
    31081 tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta
    31141 acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa
    31201 gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat
    31261 gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga
    31321 attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa
    31381 acaatgattc cttccgcact tccactgatg acattacttc ccataaagac aagattttcg
    31441 taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag
    31501 ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata
    31561 tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc
    31621 ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat
    31681 ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg
    31741 acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct
    31801 aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc
    31861 gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata tgaaatcgca
    31921 ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat
    31981 gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt
    32041 tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct
    32101 cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga
    32161 gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga atgactgtca
    32221 tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc
    32281 gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg
    32341 gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa
    32401 agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc
    32461 ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc
    32521 tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc
    32581 ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt
    32641 ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc
    32701 attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga
    32761 acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcatccaa
    32821 cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa
    32881 ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat
    32941 tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc
    33001 gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac gatccgagga
    33061 gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg
    33121 gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg
    33181 gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag
    33241 ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa
    33301 ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc tttgataata
    33361 atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgcta agctccgttt
    33421 catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt
    33481 caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg
    33541 cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg
    33601 aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc
    33661 ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt
    33721 ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct taaaatgcct
    33781 acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat
    33841 tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc
    33901 tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt
    33961 cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt ccgctaattg
    34021 ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat
    34081 agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt aaaaaggaaa
    34141 aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct tcaagcatta
    34201 gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt tttgctgttc
    34261 aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat tttttgaaaa
    34321 gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt ccgcttcatt
    34381 tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa
    34441 gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt tttcctgatc
    34501 tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt
    34561 attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc catttcgaaa
    34621 agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa gataatcttt
    34681 atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc caccgtattc
    34741 atgattgact gtccctttag tataaccagg catatggatc atcaatggca ctcgttgcat
    34801 ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc ttgctaaatc
    34861 tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga ttgaattttg
    34921 gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat ccagataatg
    34981 tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg gaaagtcgaa
    35041 attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa
    35101 cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt tatctaatat
    35161 cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga aattgtcata
    35221 acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc catggaaaac
    35281 agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa agacattgtc
    35341 tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag tttccaacat
    35401 attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt caaagctgat
    35461 cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc cgtctacctt
    35521 catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat ttttcccttt
    35581 agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat aatctaaaac
    35641 ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat
    35701 tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg aacggtcaaa
    35761 tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc ctgaaaaaat
    35821 aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt tgcttttgat
    35881 aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga tatccagcca
    35941 gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc cttgcgacac
    36001 tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc gataaaaaat
    36061 aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc
    36121 tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag tcgcaattgg
    36181 gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa aatctgcata
    36241 gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg
    36301 attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt cacatttttt
    36361 cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta agattttttt
    36421 attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc gatgtaagcg
    36481 attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa
    36541 gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc
    36601 ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg ttctcctgcc
    36661 aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt ttgaaattgt
    36721 tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca
    36781 gctgaccctc ggtattagat tatgtcagga cgctaatttt acaacctctt ggtaaacggt
    36841 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata aggagctatt
    36901 ttttgaaatt gctccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac
    36961 ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    37021 tttgccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctgtttttc
    37081 attgtccata ggattgatat tgtccttgtg atgactcatt cgtaataatt gaggcttgat
    37141 tttcttcact tcccgttatc gtttgtccac tgccagaatc tgaaggactt gttcctgtcg
    37201 actcgttttt ttgagaagag acagttgttt tcttgacttg ttcatttgtc tttttccatt
    37261 ccgctaaacg atcggcgacc acttttttgt ctactttttc agaataagcg gtgatcactt
    37321 ttccattttt caaacgggct actttgtcgc catcatgagt caaaagcaaa acggtcaaat
    37381 gcttgtcttt aaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattaa
    37441 taaattgatt ttcttctttt ccttttggca aataaatgtc caattcgcct ttttcgtaat
    37501 tggcagttat tttatactcc gcccaccaat aagttgagaa ttcttttttg aaattatcta
    37561 ctgattcttt gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt
    37621 ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa
    37681 gaaacggtct ttttttcctc atgctttcct ccactcctat ttaattttga ctattctatt
    37741 atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact
    37801 tctttttcac aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa ctattctttc
    37861 ttaagcctca aatccgtttt tatctgatac gtctttgaac caatgacccg attttttgat
    37921 tgttcttttt ccatcttgtg ctaagtctac tgaaataaag ccatatcgat tcttatatgc
    37981 attggtccac gaccagttat ccatacatgt ccacatatga tagcctaaac aattggcacc
    38041 ttcttgaatg gcttgatgga catatttcag atgagattgg acaaattcga tccgataatc
    38101 atcgtggatc atgccatctt ctttgataaa tcgtgcttcg ttttctacac ccataccgtt
    38161 ttcagaaata aagcagtcga tatttccata atgatctcgt acattgatca aaatatcata
    38221 gattccttgt tcatagatct cccatccgcg ataaggattc attttacgac caggcatttc
    38281 ataattgtcg aaataatcct caggcattgg accgttttta gtatcgattg gtgtttcttt
    38341 tgcttttacc cggcgtggct ggtaataatt gatacctaaa agatctactg tattttcttt
    38401 gataaccgtc aaatcctctg catggatcac aggcatgaga tcaagttctt ttactatctc
    38461 aatcaattca cttggaaact cgccttttac agctggatca aggaatgaac gattgaagaa
    38521 cgcatctgct atttgcgctg ctttaacatc ttcagggttt gattcgtctc ttggatagct
    38581 tggtgtcaga ttcaagatga taccaataac accaccttgc tccatttcat ggtaagattt
    38641 gatggctaac gctgaagcca acgcttcatg atagccaact tggatcgctt ttttcatatc
    38701 tgactcttct ggataatgcc atcgatataa ataacctgct tcgactggaa cgattggttc
    38761 attatgagta aaccattttt ttactttcga accaaacaat tcaaaacaac ttttggcata
    38821 tttttcatag gcttcaaccg tttctcggtt caaccatccg cctttttctt gcagatacat
    38881 cggcatatcg aaatgataca gattcacaaa gggttcgata ccgttttcaa tcaactcatc
    38941 aaaataacga tgatagaaac gaacggcttc ttcgttgatt gttccagatt catcaggtat
    39001 caaacggctc cattggatag atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg
    39061 tacatcctct tgatacttcg tataaacttg cgaggttttt tcaggtccta cttgatcaaa
    39121 gaacttttgc ggttcttttt tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc
    39181 tgaaatcctt ccttctgttt gtgggccact tgctgcagaa ccccaccaaa aatttgctgg
    39241 aaattgatat tttttcatca aagcacctct ttttcttttt tagttcgttt tagttgattc
    39301 catgtatgat attttccatc acaatcactt tttcattttc ttcctcttct tttttgattc
    39361 gttttaacag ctgttctgct gctcgttccc cgattttatc tgttggtggc gtgatcgtga
    39421 ttttcggaag gatactcaga tcgtttttat ctaaataatc atagtgactg aacgcaatat
    39481 ccttcccgat tctgacacct tcttgattga aacattcgat ggcaccaatc gacatattgt
    39541 aattgctgac aaaaatagca gttgcccctt tttctaataa tgcttttgcc ccgtaatagc
    39601 cgctttttct agaatagtct cctgtaaaga cctctaattg gatcgatgga tcattttgga
    39661 ccgcttcttt ggctcctgct aaacgttctc ttgctacgta gtctgtttga gtggcagcaa
    39721 taaatccgat ttttttatgt ccttgttcac catgatgagc gatcactttt gctacacttt
    39781 ctcgatcatt cactaatatt gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga
    39841 taactggaat actcattttt gtgagcattt caaatccaga ccactcttct tcggataaga
    39901 agacaatcag cccatcgatc acatgcttca tcaggtattc aattttttgt tcaatttttt
    39961 ctggctgatc ggcaaacccg cttaataata agctataccc attttcttca gctactcttt
    40021 cgatccctga gacaacttcc gacccaaaac ggctggaaat cccgtttatc aaaagtccaa
    40081 tagagaagct tcgattattc ttcaaccctt tagcaatgat gttttcttta tagtccaacg
    40141 ctgaaatcgc ttctgctatt tttttcatgt tcttttcctt cagttgctga ccattcaagt
    40201 aacgggaaac tgttcccact gaaacaccag ccatcttagc cacatcacgg atcgatgcca
    40261 tacattctca ctcctgccag ttacatttga tttctctttc ttgttgttcg attatgatga
    40321 ccgcattctt tgcatagtgt tgcttgtcat caatatccat ataactaaag tcttcagtag
    40381 agcaacttcc tctcactatt aattatactt aaacaagtat ttgaaccggt tcatttttaa
    40441 tatagcattt ttttaaaaga ttgcaacact tttttaaaac gctttctgaa agtggcggat
    40501 tttttgcctt tttaaaattt tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt
    40561 tttctcttcc atccgtttga agttttacat tccaatggta taatttcttt atcctatcaa
    40621 atgggggaag tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga
    40681 cattgactgg ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt
    40741 cttctgtctt aaaagcggaa aaagcagata ccaccaacag tgtatatacg ctaaaagtac
    40801 gtaaagatat ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga
    40861 cacgcacgta tcagacacaa ggaactgatt cttctgaatc atctgaagaa aaaacagtgc
    40921 tgacagatat caagatcact accaaagatg acgaatatat tatttcagga aaagaaaata
    40981 aaaaagaaac aacagtcata tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg
    41041 gcaacatcta cagtctatag tgccgtctgt tggatcttct tttgtttctc cccttctttt
    41101 ctgctatagt aaagaagcaa gatgattgta aaggagacga aatggatgat cgatagtcct
    41161 gattattgta catgtaataa caacaaaaat atccatacag aatttaatga ccaaggaagc
    41221 ttgagcatat gcgacaactg tcacaaacta gtcaaagatt catttaaaac atttgaatct
    41281 tatcctgttc aaaattatgt aaaaagtaga agaagataaa caaatccaag caaaactcac
    41341 tatggctgat ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcatcagta
    41401 aaacaaacag tggatcattt cagtttacaa atcaaagagt aaagatttat ttatttcaaa
    41461 tatttgaata taaaataacc aaacgataga aaggcttatt ctacatcacg tag
//
LOCUS       DAZUJO010000017        39635 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000017 DAZUJO010000000
VERSION     DAZUJO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.017"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..576)
                     /locus_tag="ACG8OL_000988"
     CDS             complement(31..576)
                     /locus_tag="ACG8OL_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4816992.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(588..1280)
                     /locus_tag="ACG8OL_000989"
     CDS             complement(588..1280)
                     /locus_tag="ACG8OL_000989"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS4816993.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1299..1583)
                     /gene="macP"
                     /locus_tag="ACG8OL_000990"
     CDS             complement(1299..1583)
                     /gene="macP"
                     /locus_tag="ACG8OL_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS4816994.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1596..2159)
                     /locus_tag="ACG8OL_000991"
     CDS             complement(1596..2159)
                     /locus_tag="ACG8OL_000991"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS4816995.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2387..3049
                     /locus_tag="ACG8OL_000992"
     CDS             2387..3049
                     /locus_tag="ACG8OL_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS4816996.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3173..4369
                     /locus_tag="ACG8OL_000993"
     CDS             3173..4369
                     /locus_tag="ACG8OL_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS4816997.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4470..5846)
                     /locus_tag="ACG8OL_000994"
     CDS             complement(4470..5846)
                     /locus_tag="ACG8OL_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4816998.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5883..6749)
                     /locus_tag="ACG8OL_000995"
     CDS             complement(5883..6749)
                     /locus_tag="ACG8OL_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS4816999.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6838..7863)
                     /locus_tag="ACG8OL_000996"
     CDS             complement(6838..7863)
                     /locus_tag="ACG8OL_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4817000.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7881..8555)
                     /locus_tag="ACG8OL_000997"
     CDS             complement(7881..8555)
                     /locus_tag="ACG8OL_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4817001.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8739..9632)
                     /locus_tag="ACG8OL_000998"
     CDS             complement(8739..9632)
                     /locus_tag="ACG8OL_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4817002.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9706..11403)
                     /locus_tag="ACG8OL_000999"
     CDS             complement(9706..11403)
                     /locus_tag="ACG8OL_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4817003.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11396..12709)
                     /locus_tag="ACG8OL_001000"
     CDS             complement(11396..12709)
                     /locus_tag="ACG8OL_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4817004.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12709..16650)
                     /locus_tag="ACG8OL_001001"
     CDS             complement(12709..16650)
                     /locus_tag="ACG8OL_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4817005.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16759..17217)
                     /locus_tag="ACG8OL_001002"
     CDS             complement(16759..17217)
                     /locus_tag="ACG8OL_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4817006.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17515..18033)
                     /locus_tag="ACG8OL_001003"
     CDS             complement(17515..18033)
                     /locus_tag="ACG8OL_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4817007.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18035..18652)
                     /locus_tag="ACG8OL_001004"
     CDS             complement(18035..18652)
                     /locus_tag="ACG8OL_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4817008.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18649..19503)
                     /locus_tag="ACG8OL_001005"
     CDS             complement(18649..19503)
                     /locus_tag="ACG8OL_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817009.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19500..20411)
                     /locus_tag="ACG8OL_001006"
     CDS             complement(19500..20411)
                     /locus_tag="ACG8OL_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817010.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20452..21786)
                     /locus_tag="ACG8OL_001007"
     CDS             complement(20452..21786)
                     /locus_tag="ACG8OL_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4817011.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21994..22824
                     /locus_tag="ACG8OL_001008"
     CDS             21994..22824
                     /locus_tag="ACG8OL_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS4817012.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23068..23317)
                     /locus_tag="ACG8OL_001009"
                     /pseudo
     CDS             complement(23068..23317)
                     /locus_tag="ACG8OL_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23508..23702)
                     /locus_tag="ACG8OL_001010"
     CDS             complement(23508..23702)
                     /locus_tag="ACG8OL_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817013.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24207..24392)
                     /locus_tag="ACG8OL_001011"
     CDS             complement(24207..24392)
                     /locus_tag="ACG8OL_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817014.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25242..25583)
                     /locus_tag="ACG8OL_001012"
     CDS             complement(25242..25583)
                     /locus_tag="ACG8OL_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4817015.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25583..25813)
                     /locus_tag="ACG8OL_001013"
     CDS             complement(25583..25813)
                     /locus_tag="ACG8OL_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4817016.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26100..26438
                     /locus_tag="ACG8OL_001014"
     CDS             26100..26438
                     /locus_tag="ACG8OL_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817017.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26643..27205)
                     /locus_tag="ACG8OL_001015"
                     /pseudo
     CDS             complement(26643..27205)
                     /locus_tag="ACG8OL_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27937..28518)
                     /locus_tag="ACG8OL_001016"
     CDS             complement(27937..28518)
                     /locus_tag="ACG8OL_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4817018.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28701..29327)
                     /locus_tag="ACG8OL_001017"
     CDS             complement(28701..29327)
                     /locus_tag="ACG8OL_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS4817019.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29349..30680)
                     /locus_tag="ACG8OL_001018"
     CDS             complement(29349..30680)
                     /locus_tag="ACG8OL_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS4817020.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30835..31236)
                     /locus_tag="ACG8OL_001019"
     CDS             complement(30835..31236)
                     /locus_tag="ACG8OL_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS4817021.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31322..31528)
                     /locus_tag="ACG8OL_001020"
     CDS             complement(31322..31528)
                     /locus_tag="ACG8OL_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817022.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31623..33125)
                     /locus_tag="ACG8OL_001021"
     CDS             complement(31623..33125)
                     /locus_tag="ACG8OL_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS4817023.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33143..33598)
                     /gene="argR"
                     /locus_tag="ACG8OL_001022"
     CDS             complement(33143..33598)
                     /gene="argR"
                     /locus_tag="ACG8OL_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS4817024.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33696..34313)
                     /locus_tag="ACG8OL_001023"
     CDS             complement(33696..34313)
                     /locus_tag="ACG8OL_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4817025.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34534..35907
                     /locus_tag="ACG8OL_001024"
     CDS             34534..35907
                     /locus_tag="ACG8OL_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4817026.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35982..37094
                     /locus_tag="ACG8OL_001025"
     CDS             35982..37094
                     /locus_tag="ACG8OL_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4817027.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37166..38803)
                     /locus_tag="ACG8OL_001026"
     CDS             complement(37166..38803)
                     /locus_tag="ACG8OL_001026"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS4817028.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38971..39579)
                     /locus_tag="ACG8OL_001027"
     CDS             complement(38971..39579)
                     /locus_tag="ACG8OL_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4817029.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11374 a         8438 c         6885 g        12938 t
ORIGIN      
        1 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
       61 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg aatctttgaa
      121 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
      181 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
      241 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
      301 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
      361 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
      421 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
      481 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
      541 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
      601 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
      661 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
      721 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
      781 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
      841 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
      901 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
      961 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
     1021 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
     1081 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
     1141 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
     1201 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
     1261 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
     1321 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
     1381 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
     1441 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
     1501 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
     1561 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
     1621 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
     1681 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
     1741 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
     1801 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
     1861 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
     1921 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
     1981 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
     2041 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
     2101 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
     2161 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
     2221 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
     2281 tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa
     2341 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
     2401 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
     2461 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
     2521 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
     2581 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
     2641 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
     2701 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
     2761 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
     2821 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
     2881 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
     2941 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
     3001 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
     3061 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
     3121 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
     3181 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
     3241 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
     3301 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
     3361 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
     3421 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
     3481 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
     3541 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
     3601 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
     3661 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
     3721 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
     3781 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
     3841 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
     3901 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
     3961 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
     4021 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
     4081 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
     4141 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
     4201 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
     4261 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
     4321 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
     4381 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
     4441 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
     4501 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
     4561 acagtttgca gacgaagcat caatacaata caagtcaaaa tggagacgat tggcaatagc
     4621 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
     4681 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
     4741 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
     4801 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
     4861 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
     4921 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
     4981 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
     5041 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
     5101 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
     5161 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
     5221 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
     5281 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
     5341 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
     5401 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
     5461 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
     5521 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
     5581 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
     5641 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
     5701 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
     5761 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
     5821 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
     5881 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
     5941 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
     6001 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
     6061 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
     6121 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
     6181 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
     6241 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
     6301 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
     6361 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
     6421 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
     6481 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
     6541 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
     6601 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
     6661 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
     6721 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
     6781 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
     6841 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
     6901 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
     6961 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
     7021 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
     7081 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
     7141 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
     7201 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
     7261 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
     7321 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
     7381 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
     7441 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
     7501 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
     7561 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
     7621 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
     7681 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
     7741 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
     7801 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
     7861 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
     7921 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
     7981 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
     8041 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
     8101 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
     8161 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
     8221 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
     8281 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
     8341 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
     8401 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
     8461 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
     8521 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
     8581 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
     8641 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
     8701 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
     8761 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
     8821 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
     8881 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
     8941 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
     9001 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
     9061 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
     9121 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
     9181 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
     9241 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
     9301 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
     9361 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
     9421 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
     9481 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
     9541 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
     9601 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
     9661 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
     9721 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
     9781 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
     9841 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
     9901 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
     9961 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    10021 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    10081 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    10141 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    10201 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    10261 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    10321 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    10381 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    10441 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    10501 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    10561 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    10621 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    10681 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    10741 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    10801 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    10861 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    10921 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    10981 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    11041 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    11101 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    11161 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    11221 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    11281 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    11341 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    11401 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    11461 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    11521 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    11581 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    11641 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    11701 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    11761 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    11821 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    11881 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    11941 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    12001 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    12061 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    12121 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    12181 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    12241 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    12301 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    12361 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    12421 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    12481 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    12541 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    12601 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    12661 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    12721 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    12781 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    12841 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    12901 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    12961 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    13021 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    13081 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    13141 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    13201 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    13261 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    13321 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    13381 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    13441 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    13501 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
    13561 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    13621 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    13681 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    13741 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    13801 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    13861 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    13921 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    13981 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    14041 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    14101 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    14161 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    14221 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    14281 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    14341 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    14401 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    14461 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    14521 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    14581 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    14641 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    14701 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    14761 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    14821 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    14881 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    14941 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    15001 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    15061 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    15121 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    15181 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    15241 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    15301 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    15361 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    15421 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    15481 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    15541 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    15601 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    15661 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    15721 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    15781 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    15841 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    15901 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    15961 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    16021 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    16081 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    16141 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    16201 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    16261 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    16321 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    16381 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    16441 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc
    16501 gttttcaact tctccgctag gatagatata ttcatagggg tcttgcgtag ctgttggccc
    16561 aagacgctcc aaaataccgt tgaataactg gcgttgaatg cgctggagtg cttgaccgct
    16621 gggtacgatt ggttcgtcca gcttttccat ctcattttct tcgctacgtt gaatttcagc
    16681 ggaatcttct gtatcagttt cattttcttg taattcttgg ttatctattc tttcatcaac
    16741 caccacttga agaggtagtt acccttcgtt cacatcgggg atcttttctg tttcaaaaat
    16801 caatgtgccg gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc
    16861 gatcgtctcg acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg
    16921 aacgtttttt acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc
    16981 ttcattggca ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc
    17041 aatatacttc ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt
    17101 ttctgttgac tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg
    17161 tccaaatagc aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc
    17221 cttatactcc tttcaaaaat atttgtcttc tttcacaaca atcaacaaaa caatgcccat
    17281 ctaattataa tttaatttaa ttttaatttt aaatcaaatg atatcctttt tagttaaaaa
    17341 acaccaatta tacaacattt attattaaaa ttaaattata aaattttata acgttctcat
    17401 ttaacaaaac ttaagttatt aagacaaatg aataaacaac tacgctttcc tcgaaacaaa
    17461 aaaataaagc acaaatgcgc atttacgcac tcatgcttta ttcgtacaat taattcagat
    17521 aacggcagcg cataggatgc caataacact tcctaaaata gctcctccta aaatgtcagt
    17581 aggataatgt aaaagtaaaa aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc
    17641 taatattcct gcaactggac ttaattcgaa caacgcagct actgataaaa aggctagcgc
    17701 tgtatgtcct gaaggaaaag aagaagtctt cggtttactt gtgtaaagga caagccgacg
    17761 atttttttca taaggtcttt tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa
    17821 aaaatagatc aaactgccga taagaatggc tagaccatat tctctagtat caggtatcaa
    17881 gactaaaaca gcagcaagga gcaaccaaat aaaaccacca ttagagatat tactgataaa
    17941 tttcaaaaaa ttgatttttc gactatccgt tttccgttgc ttggcgaact tgttgataat
    18001 actatcatcg attttagcaa tcaaacgcaa catgttattc ctccttttta agaacctttt
    18061 cttgcgtacg atggataaaa agcaggactt gaaaataaaa caagaaactg aaacctaata
    18121 aagcaaaata gattggtcct gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca
    18181 aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa aaatgcagta atccaagaag
    18241 cttctttttg ttccagttga agcaagattt tgtaactaaa gagaagcgcg cttaatagaa
    18301 gcaaaaacaa cgtagtatac atcaatgtat agccaattcc cccaccccaa tagcggaaaa
    18361 tctgataatc gatcaataga agaagaaaat aaacacttgc taaactatct tgctttccat
    18421 aaagagagaa gttctcacga aactttttcc agaatagtga accgagcttt ttctcttcat
    18481 tttttttgta atgataaatc atatacagaa ctgttcgact tgcagttcct aatgtgaaaa
    18541 caggcaagac gcacaggaac catagtagct gtaaatggat aagacgtgga atccattcaa
    18601 tgatttctaa gaaataatca ttggcttttt taccaatcga tcgattcatc atccttttga
    18661 acctcctgtc aaatccatcc cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat
    18721 cgtcgggatc aagacaagtg ttgctcctgc catcagataa ttccattgtg tcgttgcttg
    18781 gttctgaaaa acttgcaagc caatctgtaa cgtgtatttt tcttgatctt ggatatataa
    18841 cagaggacct aagaaatcat tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa
    18901 ggcaggtttc gtcaatggaa gcatcaaatg actccaaata tacaaatgat tcgctccgtc
    18961 gatttcagca gcttcgatca attcattatt gatcgataag taaaattgac gcatcagaaa
    19021 tatattgaac gcattgccga agaacgatgg aacgatcagg ggcaaataag tacctaccca
    19081 gccgatcttg gagaataata catattgagg gatcatcgtt acaaaccccg ggatcatcat
    19141 cgtagaaaga accaaagcaa acatcagctt tttcccagga aaatcaagct tcgcaaatcc
    19201 ataagcaata aaagaatttg aaaggacatt accgatcact actagcacag tgatgaataa
    19261 tgtatttctt gcatagcgcc aaaaaggaaa ggcggcaatc gtatccagat agttttccca
    19321 tacgattttt tctgggaaaa aggtcggagg ataacgaacg atttcttcca ttggtttcaa
    19381 tgaagtaaac accatccaaa gcaatggaat gatcaaaatg attcctatta ctgtcatgag
    19441 gacataactt agtatttttt ttgtttgccg attcacactg gtctttttga aataatccat
    19501 catttgccgt cacctccaga ataatagacc cacttaggtg caagcttcaa attgacaaac
    19561 gtcagaacca acacgatcag aaacaggatc attgacatag ccatcgcata acccatatca
    19621 aaagctacga acgccttgtt ccacatatgc agattataaa aaagcaaaga atttgcaggt
    19681 ccgcctgttc catttttcgt catgacataa gcttcttgaa aaatttggaa agaaccgatc
    19741 gtagaggtga tcacatcgta aaaaataata ggagtgatta atggcaaagt aatacttttg
    19801 aactttcccc aaacgcctgc tccatcgatt tctgctgatt catataaaga tctaggaaca
    19861 ttttgtaatg tagctaagta aagtagcatt gctcctccag aactccatat tttcatcaaa
    19921 atcaacgcag gtttcgtcca ttgtggatca aacagccaag ctggtccagg gatatggaac
    19981 cagcccagaa atgtgttgat caatcctgca gatggagaaa gcagctgcat ccataagatg
    20041 tagactgcta ctcctgataa gactgccggt aaatagaaag ctgtacggaa tacactgatt
    20101 cctttgattt tttgattgag tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt
    20161 acattgaaga tgacgaaata aacggtgttg tataacgatt tccaaaataa atcatcgtga
    20221 acgaacattc ttttgaaatt tgctagacca atgaaattca tcttggatgt caaattataa
    20281 tcggtgaaac taccaataac tgaaaaaagc ataggcccta gcataaatac caagaacccg
    20341 ataataaaag gcgagataaa taaataggcc catcttgctt cttttttctt tcggatagat
    20401 aaacgcttca aattttcttg cctcctctgt acctagacag tcaaaaatgg attattgatt
    20461 ttgcttgact aagttttcta ctgctttttg tgcatctgcc aatccttctt ttggtgattt
    20521 tgttcctaaa agaatctggt caatctgttc attcactaat gtcgtgtaat ccggagcatc
    20581 taaaggaact ggtgtgatca ccgtattttt aaagttttcg tccgccattt gatagatcat
    20641 ttttccttta tccgttaggt tgtcattatt cactaaattc tcattggctg tcttgctcgc
    20701 catgatatca aaactttttt cgccgaattt ttcttgtact tctggagaag aaagatattt
    20761 gatgaattca taagaggcct caggatcttt tgctccgtat ggtacttcca agacgaatcc
    20821 gccaccccac gaccaatggc ctgaaccttc ttctttttct gggagctgca tcacgccaaa
    20881 atcaaaatca tccggtgcat tttccatcaa actagagtaa taattgatat tttgggcacg
    20941 catggctact aatccggaaa taaatggatc agatacacca gacccaaatt cggcttctaa
    21001 tttattgatc gtatttttcc cataataatc ttgccagtct tggatccact ccaaggcttc
    21061 cactttattt ttagtatcaa tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt
    21121 atctgcgttt agcgcccata catcagcccc taaattccat aatggataaa aaccgatgcg
    21181 tgtaaattct tttccttctt ttttatctag tttacgtgct gtttcttcta attcatccca
    21241 agttgtcggt agatcttgtt cactgattcc agcttcttta aataactttt tattataaaa
    21301 aatgacttgt gtatctgtat tgaatgggat cgcataagga tcaccttcat attctactgt
    21361 atcccaaagc tgcggataaa aatccttaga agtgtctttt tctacatatt tggagagatt
    21421 agttgcttgc tgtgcttctg ctctttgggc aacggaattt atgtcctgta caataacatc
    21481 gggtgcattt ccagctgtta ttgcagataa agatttagtc cagatgtcac cccatggctg
    21541 atacttataa tttacatgga tctcgttttg tgattgattg aaatcctcaa tgatttcttc
    21601 gatagcttct tttcgtgcac cggatcccca aaaggaccag aaatcaatat tttttcttcc
    21661 atcacttgtt ttttcagaag aatttgctgc acttgaactg gcatcgcttg cattacttga
    21721 tccgcatcct cctaataaaa acaatgctga acttgccagc aaccctgtaa acaataattt
    21781 tttcatattt tacctccaaa ttttaatgta accgatgccg gttttaatat aacgatatca
    21841 ttttgttctg aaaataacaa tttataaggt atgattctaa aaaaattatt aatgatacca
    21901 atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt agcagaatga
    21961 gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa agaattgcag
    22021 tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca ggagctgatc
    22081 ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg ctggagctgc
    22141 cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga aatcggccac
    22201 gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa
    22261 gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat gaatccacag
    22321 cacgcgatcg tagctggctt aggacctcgt cctgctgaca gcgtgggcaa tccatggagc
    22381 ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa tttaaatgcc
    22441 gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga tcgtggcgta
    22501 aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct atgggaatgt
    22561 gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt
    22621 gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg agaccaaagt
    22681 agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata tcaatggttg
    22741 aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc aaaaatgtat
    22801 gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt tttgttccac
    22861 acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca aaagggtggt
    22921 tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg gctttcatat
    22981 tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag gtttatgtac
    23041 tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat ctaacgcact
    23101 atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt gtcctacttt
    23161 tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt ttagatttgc
    23221 acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt atttttcttc
    23281 tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg gctaaactat
    23341 ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc tcggcgcgct
    23401 caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag aaacaacggg
    23461 ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta tttttttgat
    23521 tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata aaaacaagaa
    23581 cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact agccaacaat
    23641 atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag tgttgttttc
    23701 atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt actagaaaaa
    23761 ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc aggccatctt
    23821 gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat ttgtggcggt
    23881 gacctccaat ttttaactct tctatctccc atccagtata cataaccttg atacttcgac
    23941 cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct aacttagttg
    24001 ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa gtatctgaac
    24061 tattaatttt taaactatca tttttatcct caactacatg cacatctgtt gcaacttcac
    24121 tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct tcagctgacg
    24181 ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc aacagcaaaa
    24241 aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc
    24301 aactcattac tgtatgattt tagcagccca accaacatat accctataaa agctaataag
    24361 atggttagga atcctttaga atttttactc attcatagac acttccttaa ataactttta
    24421 ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata aaaaaaacct
    24481 tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact gacataagat
    24541 cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta gagaataaac
    24601 taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac tttggttcac
    24661 tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa
    24721 ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga tgttccagtg
    24781 atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt tgtgttattc
    24841 agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta cgtttttgag
    24901 catcgatata gtttaaaatg acattactca tatctaaaga gtttagattt tttaggacta
    24961 aaactgtcag ttgttctttt tcttctttct gttaaagtat tgtcgcgact tttcgtatac
    25021 gttaagatat gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa
    25081 atcacctcaa tcattcgttt aaaatgctat atattcttta gtttaagata cggaaattaa
    25141 tgccatcgac cacactagta gaatgaacga catttacttg gccttttttt aatttcgtta
    25201 tgtttagcat ataaaaatat tatagagttt acttacaatg cttaaaacat atactgtata
    25261 atctgatcaa ttttagccat gtcttgcaat ggtaagtttt ctattttttt gagttttcgt
    25321 tccttaaaat caagagattt taattgtgta ataattactt gtccttttat atctagctct
    25381 tcaggtaatg agtaacgtgt cggtaaattc tttatagtgg aagtaattgg acatatgacc
    25441 gaaaacattg ttttacggtt aaattcataa cgagagacga ctagtcctgg tcgtcttttt
    25501 tgaatttctt ttcctacgga gggatcaaaa tcaatccata caatatcacc ttttttagga
    25561 atataaattc tttcttctga cactataaaa tttctctccc ttcaggagtt aaatcttccc
    25621 attcgtcttt ctcataatac tcggcctctt ccccaccact aaatggatct tcaatttttg
    25681 gaactaacgt aattgttcca tcatcagaat acacaacgat atattcttgg ttttctgaag
    25741 gtttctttcc gttatcagaa ggtaaagtta ccactacaga acttccttgt aatctagatt
    25801 ttgtaacaag cattatcatc actcccttaa taaaagtata ctatataaca ctaagtaacg
    25861 caaaattccc ctttgtaaac tatctagaaa attgttttgt ttaaagtata tcttattgga
    25921 tataaataga aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag
    25981 aaacattgaa cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct
    26041 tcaattttgc agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca
    26101 tgcccgatta taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa
    26161 agttcacgaa actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc
    26221 aaatagaaac attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg
    26281 agatcgtagc tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg
    26341 ataaatggca acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa
    26401 ctttgaattt tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa
    26461 ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga
    26521 ctgtgaccct atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt
    26581 ttgggatctg tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta
    26641 tgttatacct tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa
    26701 gaaatatctg ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca
    26761 tggattggtg cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt
    26821 tcatcctgat ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac
    26881 ataaatttgt ctttttcttc attccactca ttgggaaaac acaacgattt tgcaaaaatg
    26941 gttgatgaaa ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc
    27001 cttttttaaa tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct
    27061 cttttcttaa cgtcacaaca agatttgacg gaaaaacttc tccagctgct aattctgctg
    27121 cctgcttcgg ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta
    27181 gatcaaaaaa ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac
    27241 tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt
    27301 gacaagtgac aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca
    27361 caatattcaa caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa
    27421 acggcaggct tacgacctct gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc
    27481 caaatttatg caaaaatgta gaaagctatt ttcacaaaat ttttcttaat actcgaaact
    27541 aagcgacttt ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat
    27601 aagcccaacc aaacgaaaaa tatgagcagc tatttttatt tggctgttct tattacttga
    27661 tgcagggcag ctttttcaca acctctccac taaacggtgt tcaatcagct aaccttcggt
    27721 attagctcaa aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc
    27781 aggtcaaagc gctgatttca caacctctaa tacacggtgt tccagaagta gcttcttcga
    27841 aaaataagcc gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    27901 cttgaagctg accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa
    27961 caatctttct ttgatcaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt
    28021 caacaataag atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc
    28081 ttcatcggac agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca
    28141 ttgattgact aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat
    28201 aatcaaatta cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat
    28261 ccagtccaac atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc
    28321 gaacaattct gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt
    28381 ttttgaagaa aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc
    28441 tcggattgac gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat
    28501 cttttccttt gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca
    28561 gtaaattcat cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca
    28621 cagccctcac tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga
    28681 ggacaaaagc aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact
    28741 tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca
    28801 ccaattgcat ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg
    28861 gaatcgctac acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt
    28921 ctcaattgga gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg
    28981 atcgtatttt catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct
    29041 tccactgatt catattgact ttttctagca aacatctgct gctcaagcat catatgttct
    29101 cctgcaccgc caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag
    29161 agtttgtatc ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg
    29221 tatttttcta agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg
    29281 acttgcgggt cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca
    29341 ttcgttattt attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt
    29401 tgatctctca ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg
    29461 acttcatgag aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg
    29521 actaaaactt tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt
    29581 gtcatatact tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa
    29641 cctacattgg ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc
    29701 gttgttcgtt tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag
    29761 atataagtga actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct
    29821 gtacgaaggt attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt
    29881 tctaatccaa gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct
    29941 tggattgtta cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct
    30001 gctacttgat ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct
    30061 aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga
    30121 acgacaactt tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca
    30181 gccccgatga tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca
    30241 atcaagtagc gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta
    30301 ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt
    30361 tgatggttag aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg
    30421 atcatatgat aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc
    30481 tctgtaaagt cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg
    30541 atgttgatac atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca
    30601 ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata
    30661 ttttgatatt tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa
    30721 atatgataac cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc
    30781 agacaaaaag cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac
    30841 aatcgttact tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg
    30901 cgcatcttct gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa
    30961 tactacaccg atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac
    31021 tgtggactct gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca
    31081 agaagcataa cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg
    31141 aggagtgatt ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc
    31201 atggttgatc attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca
    31261 tttgctactt ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg
    31321 cttattcgaa taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac
    31381 tcgtgaacaa cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt
    31441 ttatatgatt gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg
    31501 ttaaaaaaat aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc
    31561 actcgattta accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg
    31621 gtttattctg ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt
    31681 actaacgcca acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc
    31741 tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat
    31801 cgaatacctt cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc
    31861 ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat
    31921 cggatttctt gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata
    31981 ccttttcttt ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca
    32041 tccagaaaat agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta
    32101 tctttcccaa gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata
    32161 taacgaactt ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg
    32221 gaattcgtca cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg
    32281 atcaaattat ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca
    32341 tctgtatcga aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt
    32401 tccatcgctt ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc
    32461 aaaaattggg aacctgttga atccgcacct aagctattca atactttttc agcaaagaat
    32521 ccaccagcac catttccggc atcaacaatg attttccaac catttagtgg tttgctttct
    32581 agtgttccaa tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg
    32641 tctgcttttt ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata
    32701 aaagcaatat cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa
    32761 taatagggaa gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat
    32821 tgagtggaca taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct
    32881 tgttctttca gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca
    32941 tgaccaatcc caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc
    33001 cagtttgcta gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat
    33061 ccttcaactg gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct
    33121 ttcatataaa agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct
    33181 gtgcttcttc ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga
    33241 caacttcagg gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt
    33301 cagtcaatac gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg
    33361 ctgtacgcaa acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta
    33421 ctattttgtt ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg
    33481 ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc
    33541 caataggttg ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat
    33601 tagacacctc tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta
    33661 ttttttactt ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc
    33721 aaaatcgtct tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg
    33781 tgttctttag aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac
    33841 gttgctggtt cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc
    33901 atctttttga atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg
    33961 gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa
    34021 aaatgttctt ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct
    34081 tccagtggat agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac
    34141 atgcgtattg catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga
    34201 ttcgggtcgc cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc
    34261 gggctttttt tcatactata gctcttgtct ttacgccatt caattttttc catttttctt
    34321 cctccgcgaa attccattta gttatttatt atcatacctc tttttgagtg acttggctaa
    34381 tcgtgttgac actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga
    34441 taacacgttt cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct
    34501 ctttttttac gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta
    34561 gtacctgcag taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc
    34621 atgaatgtaa ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa
    34681 tgggtaacaa cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg
    34741 aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt
    34801 ataatgatcg atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa
    34861 ggtgcagcga caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc
    34921 ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca
    34981 attggcccca cttatggagg cattatgact tcacatttta catggaatca tatcttcctg
    35041 tttcttgtgc ccgttttgtt tctttctttg attatcggct ggtttgctat tcccgagatg
    35101 gcagtacaaa aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt
    35161 agtggaagtc taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc
    35221 atcggcggta ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg
    35281 atccgactgt ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt
    35341 tatcagtttt tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa
    35401 ggaagtagtc cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg
    35461 ctttctcctt tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct
    35521 gggctgattc tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca
    35581 gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc
    35641 aatatgatga ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca
    35701 atgttcaata ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt
    35761 atcaattacg tccagcatca tacttcccat aactatgaag tgtctactac attaggatca
    35821 aaagcagcat taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat
    35881 tatctgttct ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt
    35941 tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta
    36001 tcggtggcgg gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg
    36061 ttgctttatt tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc
    36121 cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt
    36181 ttttccctaa acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga
    36241 cacttttgct tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa
    36301 aagaagaagc gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag
    36361 cacttccgtt attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg
    36421 gaaaagcata tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc
    36481 aggcaaaagt cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag
    36541 aatacacagc tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag
    36601 aattaggcta tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat
    36661 cttataagat gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc
    36721 ctttcatgga tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt
    36781 tggctcctac tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg
    36841 atccagatca gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt
    36901 cagatttttc cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg
    36961 gtctaggctc ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct
    37021 taaatactgg tcattggcct acggaagatt atcaaaagga catctccacc tatattacga
    37081 aaaatccatc atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg
    37141 attacttctt gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt
    37201 cttttgcttc atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa
    37261 tatcaagtcg ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat
    37321 ctggtatagg taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt
    37381 gttgcacgat tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga
    37441 aatatccagt attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag
    37501 cttgatagtc ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta
    37561 gctcatctag ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg
    37621 ttgcaccaaa agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat
    37681 catctttcat gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg
    37741 gtgtcgcata acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta
    37801 ggactttttc tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag
    37861 aaatggccgg atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg
    37921 cagtagcaaa accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag
    37981 agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa
    38041 atacagccat cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg
    38101 caataaagcc attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt
    38161 ctccaaagta acgtaatcct agcacagccg ctgtattatg gagagcatca aacaaaatga
    38221 gtccatttgg aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct
    38281 catcaaagga aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat
    38341 tcagcaaata agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca
    38401 cagaaaattc ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac
    38461 tttgatacac agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct
    38521 caaagatccg ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg
    38581 tcctaacatc agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga
    38641 tcgttcctgg actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa
    38701 cattattacc gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc
    38761 cacttgtgat ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc
    38821 taaacaaaaa aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc
    38881 tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt
    38941 tctcggtttc atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt
    39001 tttagcagca tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg
    39061 attggcagct gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta
    39121 acgtccgtta gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc
    39181 agagcttgaa gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc
    39241 aacaggtgca acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc
    39301 tgttgttttg ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc
    39361 accaacataa atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat
    39421 tgagttgtct cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt
    39481 ataaacttca tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc
    39541 aaaagttgta cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc
    39601 gttttttatt ctttcgacgc tattaacttt atcat
//
LOCUS       DAZUJO010000018        34952 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000018 DAZUJO010000000
VERSION     DAZUJO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.018"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACG8OL_001028"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACG8OL_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS4817030.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACG8OL_001029"
     CDS             complement(2017..2919)
                     /locus_tag="ACG8OL_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4817031.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACG8OL_001030"
     CDS             complement(2916..3608)
                     /locus_tag="ACG8OL_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS4817032.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACG8OL_001031"
     CDS             complement(3651..4292)
                     /locus_tag="ACG8OL_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS4817033.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="ACG8OL_001032"
     CDS             complement(4330..4644)
                     /locus_tag="ACG8OL_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817034.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG8OL_001033"
     CDS             complement(4632..5261)
                     /gene="pyrE"
                     /locus_tag="ACG8OL_001033"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS4817035.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG8OL_001034"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="ACG8OL_001034"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS4817036.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="ACG8OL_001035"
     CDS             complement(5981..6913)
                     /locus_tag="ACG8OL_001035"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS4817037.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="ACG8OL_001036"
     CDS             complement(6910..7686)
                     /locus_tag="ACG8OL_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS4817038.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG8OL_001037"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="ACG8OL_001037"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS4817039.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /locus_tag="ACG8OL_001038"
     CDS             complement(10930..12006)
                     /locus_tag="ACG8OL_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS4817040.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="ACG8OL_001039"
     CDS             complement(12011..13291)
                     /locus_tag="ACG8OL_001039"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS4817041.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="ACG8OL_001040"
     CDS             complement(13310..14236)
                     /locus_tag="ACG8OL_001040"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS4817042.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="ACG8OL_001041"
     CDS             complement(14249..15532)
                     /locus_tag="ACG8OL_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS4817043.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG8OL_001042"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="ACG8OL_001042"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS4817044.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="ACG8OL_001043"
     CDS             complement(16320..17231)
                     /locus_tag="ACG8OL_001043"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4817045.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG8OL_001044"
     CDS             complement(17228..17692)
                     /gene="lspA"
                     /locus_tag="ACG8OL_001044"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS4817046.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="ACG8OL_001045"
     CDS             complement(17697..18395)
                     /locus_tag="ACG8OL_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS4817047.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="ACG8OL_001046"
     CDS             complement(18382..18768)
                     /locus_tag="ACG8OL_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817048.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="ACG8OL_001047"
     CDS             complement(18915..20585)
                     /locus_tag="ACG8OL_001047"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS4817049.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="ACG8OL_001048"
     CDS             20768..21001
                     /locus_tag="ACG8OL_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4817050.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="ACG8OL_001049"
     CDS             complement(21100..21531)
                     /locus_tag="ACG8OL_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS4817051.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="ACG8OL_001050"
     CDS             21610..22023
                     /locus_tag="ACG8OL_001050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS4817052.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="ACG8OL_001051"
     CDS             complement(22061..23209)
                     /locus_tag="ACG8OL_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4817053.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="ACG8OL_001052"
     CDS             complement(23331..23927)
                     /locus_tag="ACG8OL_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4817054.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACG8OL_001053"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="ACG8OL_001053"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS4817055.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..27588
                     /locus_tag="ACG8OL_001054"
     CDS             27157..27588
                     /locus_tag="ACG8OL_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4817056.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27633..28982)
                     /locus_tag="ACG8OL_001055"
     CDS             complement(27633..28982)
                     /locus_tag="ACG8OL_001055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4817057.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29052..30215)
                     /locus_tag="ACG8OL_001056"
     CDS             complement(29052..30215)
                     /locus_tag="ACG8OL_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4817058.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30344..30667
                     /locus_tag="ACG8OL_001057"
     CDS             30344..30667
                     /locus_tag="ACG8OL_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817059.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30850..32199)
                     /locus_tag="ACG8OL_001058"
     CDS             complement(30850..32199)
                     /locus_tag="ACG8OL_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4817060.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32268..33221)
                     /locus_tag="ACG8OL_001059"
     CDS             complement(32268..33221)
                     /locus_tag="ACG8OL_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS4817061.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33323..34513)
                     /locus_tag="ACG8OL_001060"
     CDS             complement(33323..34513)
                     /locus_tag="ACG8OL_001060"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS4817062.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34713..>34952)
                     /locus_tag="ACG8OL_001061"
     CDS             complement(34713..>34952)
                     /locus_tag="ACG8OL_001061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817063.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9948 a         7638 c         6119 g        11247 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttaatta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggttagg cggtcagttg
    27421 cttgaagctg ctgaaaaacg ggcggtttca gaaggttgtc agactagtta tgtagtagct
    27481 gaaatgacgg ctgtagattt ttatgaaaaa caagggtatc atacttgcac agaacctttt
    27541 attgaagatg aagtactttg tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt
    27601 gtgacacgcc ttttgtcaca acctcttgtt ttttatttat gtgaattttt cttattttta
    27661 cgagcagtac ggctttgctg tctgcgttcg attttgcgtt cttttttatt tttttctgca
    27721 attgcccaat cgattttttt cttataacca ggcttgatct ttttcttttt cttcttcaca
    27781 agaccaatca gcgtgggatc taatacatct tttgattttt ctcgtttggc acgtctatta
    27841 cgatcgtatg ttttgacgat ttccccgttt ttgatttctt ttggttcaaa tgagacgccc
    27901 aatttttcta tcgcatcaat ggcttgctca tcatttggcg aatataacgt aatggccgtt
    27961 ccttttaagc cattacgtcc agtacgtccg acacgatgga taaagaagtc tagatcttct
    28021 ggaatttctg cattaataac atgagagaca ccttcgatat cgatccctct agcagctaaa
    28081 tcagtcgcta caacatattg atactccaga ttctgtactt gtcgcatcac tcgttttctc
    28141 tctcttgggg tgatgtctcc atgaatcttt gctactttca agccttggtt tttcaagtaa
    28201 tcggcaattt catccacatg ctgttttgta ttggcaaata cgattgctaa ataaggatga
    28261 ccaattgtca gcagttgata aatcatttga ttctcatctt taccttttgt tgaaatcagc
    28321 caattttcaa tgtcttcaga aataactgct tttggtttga tttcttcaat gattggattt
    28381 tccatgtatt ttcgtaagaa tggacgaagt ttttctggaa tagtggcaga aaaaactaaa
    28441 aactgcagct tttctggtaa tcgacttgca atttggtcta cttcatttaa aaaacccata
    28501 tccaatgtca tatccgcttc atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct
    28561 tgttcattca tcatgtctaa tattctacca ggcgtgccta ctacaacctg tggctgttgg
    28621 tgatgcagac gatttaattg acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg
    28681 atctccggtg tagaaaattt agcgatttgt gtagctgctt catagatctg tgcagctagt
    28741 tccctacttg gagctgtaat aacaacttgt acttcctctt tttctgcatt gattttttca
    28801 aacaaaggca acaaaaacgt atgtgttttt cctgtaccag tttgagattg cccaatgata
    28861 ttttttcctt ttgcaattaa aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag
    28921 ccctttgcgg ttaacgcttc attgataaac ggtttgaagc ggaatatatc aaaattattc
    28981 attcgtgcac ctcttctctt taaattatca cgactttggc attataacac agacaaagcg
    29041 tttttgctca gctaatttcg agaatcatca tcctttgttt ggccaatcgt gctaacagta
    29101 tatgtgtgac tagctttttc ataaaacaga atccgttttt ttctcaaaaa tgatagcaag
    29161 atatttaaaa taaaaatacc ttgtagtaac aagacaccga gagaaatgaa tactgcactc
    29221 atctgcaaca ccgtattcgt tgtcgaattc atgatttgct gaagcaactg ccccaaccag
    29281 cttaaaccat aaaaaagaaa gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa
    29341 attcgttcgc ggtcttcttc caccaaacgg atacgcacaa tttttttgcc aagtgtccat
    29401 ccattggtta taaacggtac aatcataaag tataccccta cttctaataa aagccaccag
    29461 atatgagaag taactgcccc tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa
    29521 ttaataagag ataaaaatgt ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag
    29581 acagtctttc ctttttcata agaaagttca tccattcgtc ttcttgtagg aagcataaat
    29641 gtaaataacg gcgtaacgac aaaaccaatg attccaccca atgtatttgt cactaaatca
    29701 ttcacatcaa ataagcggta tggtcttggg tagataaaat aaagtccact tagctgcgtc
    29761 aattcaaaaa atagagaaag gcagaaacta gcaatcatcg ttttcataaa agaaagccgg
    29821 aaataatatc ttaaataaat gccaaacggt atgagtaata aaacattaaa agctggttcc
    29881 aaaaatacac tctgcttcat tgccggcagg taggtactag gttcagacaa gaccaatacc
    29941 gtgttgtctc tgaaatgttg ccatgaagca aataaatgca gctccatctt tggccctgtc
    30001 atttgggcaa ccgtctcctt tgccggaagt ggtaagatca ccaaaaaata cgcacacaat
    30061 aaataaaaaa cgaaagaata caaaataact gcccttgtta ctagaaatgt tccatacttt
    30121 cgatattcat aaataaaaaa aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc
    30181 agcgcaaaac gaatcggttc gatataactt gccatatgac tcctccttga ttctttgtat
    30241 ctctcttatc ttagcagtcc tttgctgttt caagcaagta acgaggctat tatttctcat
    30301 ttctttcaaa gtaaagtaag ataaacacaa agggggtgtc gagatgctga atatgtactt
    30361 tgtttttggc gtacctattt ttttactgtt cctttatgct acgatcgctt atgtaagaaa
    30421 aagaactaca attcattatt taggatttat tctcctgatt atttcgggtt tcatgctagt
    30481 cttcaatctt cagacttggc agcaagcact tcttgaaatg gataaaatga ctccacatgc
    30541 tttgtctaaa gtgcttggct accctgtcta tctgatttgg cttcccattt tcatttctgg
    30601 ttgcttggtt ttattaaata tttatcgcgg cgtacgccga atagttcagt tacgcaaatc
    30661 aaagtaaaag acagaaatcg gttgagcccg atttctgtct tttactttgc tatttcatcg
    30721 tagacaaaac gacatttacg tgatcaaaat taacgagtac ttttgacatt tcttccacgt
    30781 actcctcatt taaataaaat attttgtttg gcagagaagt gcagttctaa taaaattcga
    30841 atgttttagc tatctacgtt ttaaatagag ataaatcggg ataccagcaa gtgtcaatac
    30901 tatccccatc gaagctaaaa aggtctgagt gataagcgtg cttgatacga taaaagtccc
    30961 tccaatgatt gctactagtg gaataaacgg atacattgga actttgtatg gtcgaaacaa
    31021 atcaggttct tttttacgaa gaagaatgac accaacaaag accatcgtat aaaaaatcca
    31081 aatgacaaag atcagcatat ccgttagtgt atcaaaacca ccgatcatca tcatgccaat
    31141 agcgatgact agctctaaga tacctgcggc tacaggaact ttagttttat catgaagttt
    31201 agcaaagagc ttgctaaaag gcaggttgtt ttcttttgcc ataacataag ggagacgcat
    31261 tccagtaagg gtatagccat tgatggttcc ataaacagaa atcaagatac caactgtgac
    31321 cagtcttcca ccgaagccgc caaaaatcat ttttgcaacg tcacttgccg cattgctgtt
    31381 tcctgcgact ccgtcgatag atgcggttct taagaacacc gcatttacta ataaataaac
    31441 gatcatgatt ccgatgattc ctaaagaaat tgcttttggc aaatctttcg ccggcttttt
    31501 caattctccc gaaatatttc ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag
    31561 caagcccgct cccaaggctg agaaaaatga aagattttct cctgcttgga tcggaaacag
    31621 ttggaagtct actccttctt gtcggaacaa accgaaaatg acgatcacaa acaaaggaat
    31681 aagcttgcag acaagcgtaa tcgactgaaa agcccctcca gctttagaac ctaaaaaatt
    31741 gatcaacagg atcgatacag cagctgtaac agctacggga acaatcattg actgggaaag
    31801 accaaataga ttgacgaact gtgtaccgaa aatgatcgac aaggctgcta catttgctgg
    31861 aaaataaatc acgacttgcg cccagcccaa taaaaaagct gctgtatttc cgtaaatccg
    31921 ttcgatatat ttaatcatcc ctcctgtttc tggaatagca gctgccagtt cagctcctgt
    31981 tagtcctgca cagacagaaa tcataccacc gagaaaccaa gaaaacatat gcaaactggc
    32041 ggatcctgta acttctgcta cgcttgcggc tttaaaaaat acccctgcac cgatcactgt
    32101 gcccataaca gtcgataaag cagtaaaaaa tcccatcgta cgttttaatt cattttctat
    32161 ttttacttct tttactggcg tggcttgata ttcttccatc ccttgtcccc tttcatacca
    32221 tagccaatcc caaaagtata aaaaactttt tggatgtggt tcttgtttta taccgtctct
    32281 accaattttt ctttcagttt ttccaacata tcctttgtca tcacatctaa gtcgtatttc
    32341 ggcgaaaagc cccattcctc ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct
    32401 gctattgact gacgtacagg atccacatca taatccattt caaaatctgg tatgattttt
    32461 tgaatagatg ctttgatctc ttccgggtca aaagacatcg ccgatatatt gaaggcattt
    32521 cgatgaacca atcgatcagg atctgcattc atgagtttta taatggcatc gatcgcatct
    32581 ggcatataca tcatatccat tgatgtacct tgatcaatga aactagtgta ctttccttct
    32641 ttgattgctg aataataaat atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt
    32701 gcataagaaa tcaatccagg gaaacggaca ccacgagtat ctacgccata tttcacataa
    32761 taataatcac atagtagttc tccagctacc ttcgttaccc catacatggt tgtaggacgc
    32821 tgaagagtat cttgtggggt atgatctttt ggagtatttg gaccaaacgc gccgattgaa
    32881 cttggcgtaa aaaatttcag ctggaattct ctagcgactt ccaatgcatt gaccagccca
    32941 gtcacgttca aattccaggc taattgtggt tttgcttctg caacagctga caaaagagca
    33001 gccaaatgaa tcaatgtgtc cacttgatat tgttctgcta tgctgcgcat tttttcgtaa
    33061 tccattacat ctaggatctc aaagatatcg tttttctcaa tgacttcagg catgcgtata
    33121 tctgtcgcaa tgacattttc actgcctagt tcttgacgta gccgtgtgat caactctgaa
    33181 ccaatttgtc ctaaacatcc tgtcaccaat acttttttca tgatgatcct ccctgatctt
    33241 ttttactact atcttcagat gattaacaat atatgcgtga acatgttctc taattttaaa
    33301 tagacgaaaa aactaaattt agttaaataa tttccatttc ttttcctatt ttttgataaa
    33361 ctgctactgc ttcatcaagc atttccctcg tatgttccgc agtcggcata tttctgatcc
    33421 tgcctgtccc aagaggcaca gttggataaa cgattggttt agcataaatc ccatgttcga
    33481 tcagcttctt agagaaagtc tgagtcactt tttcgtctcc caaaatgact ggagtaatag
    33541 gtgtttcaga cataccgatg ttatagccta cttttttcaa ttcttcttta aaataattag
    33601 cattttccca aactttttcg acaagttccg gatgttcttg catcaaagta atcgaagcca
    33661 atgcagctgc agctgcacct ggagttagcg aagtagaaaa taggaatggc cgacttctag
    33721 ctttcaacca gtcgattagt gttttcgatc cagctacata acccccaaca acacctatag
    33781 ctttcgataa cgtgcccatt tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg
    33841 ttcctgcacc ttttccagtt acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc
    33901 catatttttc aacaatcgga ataatttccg gcaatcgtgc aatgtctcca tccatggaga
    33961 aaacaccatc tgtgatatac atgatttttt tatatttctt actttctatc gcttcctttg
    34021 cttttttctc aaggtctttc atatcttgat gtttaattcg gatgattttt gctccggata
    34081 agcggcaacc gtcaataata gatgcatggt ttaattcatc cgataagata gcatcttctt
    34141 ttgtcattac tgcagagata gcgcccatat tgcagttgaa tccagattga aaggcgattg
    34201 ctgcttctgt ccctttgaat tctgcgattt tttcttctaa ttgctgatgg attttcagcg
    34261 aaccattgat cgttcggact gctcctgctc ctacaccata agtttcggtt gcttcaatac
    34321 atgctttttt caattcttcg cgattggcaa atccaagata attgttggat gctagattga
    34381 tcatcttttt tccgtctacg atgatacaag caccattttc actttctagt atgtcgatcg
    34441 tattgtataa ccccttttct ttcagttgat ccaattcttt ctctaaatac attgttagtt
    34501 ctttggaaga cattgtctcc acctccatat tcatctgtct tgcagaacat accaaacata
    34561 tatcgtgtca ttcatctaca aagcaagtat gctatatttt tgataacgct tacactgatt
    34621 atgagaatta tttaaccaca ttttttattt acagtatctt tttactttca aatgacttat
    34681 atagtatgaa caattacctt ttagataatg atctatttta ctctacctgg gcagataaat
    34741 acaacacaac cattgataga agccctttcg ttaaaatcaa ttgtattctg aatatttaat
    34801 ttatttattg catagaccaa taaaaataca taaaatttcc atcttcgttt atcagcaatt
    34861 tttaatttta ctctctgaat atttcttaaa agttttactt gttgaattcg aaaaaaggac
    34921 tgtgacaaaa gtcttgtcac agtcctattc tt
//
LOCUS       DAZUJO010000019        34887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000019 DAZUJO010000000
VERSION     DAZUJO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.019"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..1081)
                     /locus_tag="ACG8OL_001062"
     CDS             complement(233..1081)
                     /locus_tag="ACG8OL_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4817064.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="ACG8OL_001063"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="ACG8OL_001063"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS4817065.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="ACG8OL_001064"
     CDS             complement(3582..3761)
                     /locus_tag="ACG8OL_001064"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS4817066.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /locus_tag="ACG8OL_001065"
     CDS             complement(3808..5631)
                     /locus_tag="ACG8OL_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4817067.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /gene="helD"
                     /locus_tag="ACG8OL_001066"
     CDS             5909..8188
                     /gene="helD"
                     /locus_tag="ACG8OL_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS4817068.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="ACG8OL_001067"
     CDS             complement(8281..8928)
                     /locus_tag="ACG8OL_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817069.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="ACG8OL_001068"
     CDS             complement(9107..9826)
                     /locus_tag="ACG8OL_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4817070.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="ACG8OL_001069"
     CDS             complement(9957..11108)
                     /locus_tag="ACG8OL_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS4817071.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="ACG8OL_001070"
     CDS             complement(11325..12065)
                     /locus_tag="ACG8OL_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4817072.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="ACG8OL_001071"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="ACG8OL_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS4817073.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="ACG8OL_001072"
     CDS             complement(12449..13042)
                     /gene="yqeK"
                     /locus_tag="ACG8OL_001072"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS4817074.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13676)
                     /locus_tag="ACG8OL_001073"
     CDS             complement(13026..13676)
                     /locus_tag="ACG8OL_001073"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS4817075.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="ACG8OL_001074"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="ACG8OL_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS4817076.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="ACG8OL_001075"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="ACG8OL_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS4817077.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="ACG8OL_001076"
     CDS             complement(15119..15646)
                     /locus_tag="ACG8OL_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS4817078.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="ACG8OL_001077"
     CDS             complement(15826..16611)
                     /locus_tag="ACG8OL_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS4817079.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /gene="accD"
                     /locus_tag="ACG8OL_001078"
     CDS             complement(16604..17473)
                     /gene="accD"
                     /locus_tag="ACG8OL_001078"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS4817080.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="ACG8OL_001079"
     CDS             complement(17474..18853)
                     /locus_tag="ACG8OL_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS4817081.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="ACG8OL_001080"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="ACG8OL_001080"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4817082.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="ACG8OL_001081"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="ACG8OL_001081"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS4817083.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="ACG8OL_001082"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="ACG8OL_001082"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS4817084.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="ACG8OL_001083"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="ACG8OL_001083"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS4817085.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="ACG8OL_001084"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="ACG8OL_001084"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS4817086.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="ACG8OL_001085"
     CDS             complement(22811..23035)
                     /locus_tag="ACG8OL_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4817087.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="ACG8OL_001086"
     CDS             complement(23088..24050)
                     /locus_tag="ACG8OL_001086"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS4817088.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="ACG8OL_001087"
     CDS             complement(24060..24500)
                     /locus_tag="ACG8OL_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4817089.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="ACG8OL_001088"
     CDS             24806..25771
                     /locus_tag="ACG8OL_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS4817090.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="ACG8OL_001089"
     CDS             complement(25828..26058)
                     /locus_tag="ACG8OL_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS4817091.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="ACG8OL_001090"
     CDS             complement(26144..27031)
                     /locus_tag="ACG8OL_001090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4817092.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="ACG8OL_001091"
     CDS             complement(27120..28343)
                     /locus_tag="ACG8OL_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4817093.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29406)
                     /locus_tag="ACG8OL_001092"
     CDS             complement(28360..29406)
                     /locus_tag="ACG8OL_001092"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS4817094.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="ACG8OL_001093"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="ACG8OL_001093"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS4817095.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="ACG8OL_001094"
     CDS             complement(31297..31563)
                     /locus_tag="ACG8OL_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS4817096.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="ACG8OL_001095"
     CDS             32042..34276
                     /locus_tag="ACG8OL_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4817097.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="ACG8OL_001096"
     CDS             34424..34744
                     /locus_tag="ACG8OL_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS4817098.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9879 a         7531 c         6079 g        11398 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatctga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag aacacttttg
    34801 tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg gaaataagct
    34861 gaaattcacg aaaattgctt ttcaaat
//
LOCUS       DAZUJO010000020        34763 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000020 DAZUJO010000000
VERSION     DAZUJO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.020"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            666..1355
                     /locus_tag="ACG8OL_001097"
     CDS             666..1355
                     /locus_tag="ACG8OL_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4817099.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1342..2550
                     /locus_tag="ACG8OL_001098"
     CDS             1342..2550
                     /locus_tag="ACG8OL_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4817100.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2844..4115
                     /gene="serS"
                     /locus_tag="ACG8OL_001099"
     CDS             2844..4115
                     /gene="serS"
                     /locus_tag="ACG8OL_001099"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS4817101.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4664..6148)
                     /gene="guaB"
                     /locus_tag="ACG8OL_001100"
     CDS             complement(4664..6148)
                     /gene="guaB"
                     /locus_tag="ACG8OL_001100"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS4817102.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6653..7348)
                     /locus_tag="ACG8OL_001101"
     CDS             complement(6653..7348)
                     /locus_tag="ACG8OL_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS4817103.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7438..8538)
                     /gene="ychF"
                     /locus_tag="ACG8OL_001102"
     CDS             complement(7438..8538)
                     /gene="ychF"
                     /locus_tag="ACG8OL_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS4817104.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8547..8738)
                     /locus_tag="ACG8OL_001103"
     CDS             complement(8547..8738)
                     /locus_tag="ACG8OL_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS4817105.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8759..9649)
                     /locus_tag="ACG8OL_001104"
     CDS             complement(8759..9649)
                     /locus_tag="ACG8OL_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS4817106.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9636..10403)
                     /locus_tag="ACG8OL_001105"
     CDS             complement(9636..10403)
                     /locus_tag="ACG8OL_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4817107.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10415..11128)
                     /gene="rsmG"
                     /locus_tag="ACG8OL_001106"
     CDS             complement(10415..11128)
                     /gene="rsmG"
                     /locus_tag="ACG8OL_001106"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS4817108.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11360..13261)
                     /gene="mnmG"
                     /locus_tag="ACG8OL_001107"
     CDS             complement(11360..13261)
                     /gene="mnmG"
                     /locus_tag="ACG8OL_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS4817109.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13278..14675)
                     /gene="mnmE"
                     /locus_tag="ACG8OL_001108"
     CDS             complement(13278..14675)
                     /gene="mnmE"
                     /locus_tag="ACG8OL_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS4817110.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14974..15357
                     /locus_tag="ACG8OL_001109"
     CDS             14974..15357
                     /locus_tag="ACG8OL_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817111.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15525..16283)
                     /gene="jag"
                     /locus_tag="ACG8OL_001110"
     CDS             complement(15525..16283)
                     /gene="jag"
                     /locus_tag="ACG8OL_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS4817112.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16301..17119)
                     /locus_tag="ACG8OL_001111"
     CDS             complement(16301..17119)
                     /locus_tag="ACG8OL_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS4817113.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17116..17472)
                     /gene="rnpA"
                     /locus_tag="ACG8OL_001112"
     CDS             complement(17116..17472)
                     /gene="rnpA"
                     /locus_tag="ACG8OL_001112"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4817114.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17590..17724)
                     /gene="rpmH"
                     /locus_tag="ACG8OL_001113"
     CDS             complement(17590..17724)
                     /gene="rpmH"
                     /locus_tag="ACG8OL_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS4817115.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18248..19582
                     /gene="dnaA"
                     /locus_tag="ACG8OL_001114"
     CDS             18248..19582
                     /gene="dnaA"
                     /locus_tag="ACG8OL_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS4817116.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19780..20910
                     /gene="dnaN"
                     /locus_tag="ACG8OL_001115"
     CDS             19780..20910
                     /gene="dnaN"
                     /locus_tag="ACG8OL_001115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS4817117.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21139..21381
                     /gene="yaaA"
                     /locus_tag="ACG8OL_001116"
     CDS             21139..21381
                     /gene="yaaA"
                     /locus_tag="ACG8OL_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS4817118.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21368..22492
                     /gene="recF"
                     /locus_tag="ACG8OL_001117"
     CDS             21368..22492
                     /gene="recF"
                     /locus_tag="ACG8OL_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS4817119.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22489..24435
                     /gene="gyrB"
                     /locus_tag="ACG8OL_001118"
     CDS             22489..24435
                     /gene="gyrB"
                     /locus_tag="ACG8OL_001118"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS4817120.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24455..26926
                     /gene="gyrA"
                     /locus_tag="ACG8OL_001119"
     CDS             24455..26926
                     /gene="gyrA"
                     /locus_tag="ACG8OL_001119"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS4817121.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27162..27461
                     /gene="rpsF"
                     /locus_tag="ACG8OL_001120"
     CDS             27162..27461
                     /gene="rpsF"
                     /locus_tag="ACG8OL_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS4817122.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27511..28023
                     /gene="ssb"
                     /locus_tag="ACG8OL_001121"
     CDS             27511..28023
                     /gene="ssb"
                     /locus_tag="ACG8OL_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4817123.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28049..28285
                     /gene="rpsR"
                     /locus_tag="ACG8OL_001122"
     CDS             28049..28285
                     /gene="rpsR"
                     /locus_tag="ACG8OL_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS4817124.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28428..30401
                     /locus_tag="ACG8OL_001123"
     CDS             28428..30401
                     /locus_tag="ACG8OL_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS4817125.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30407..30859
                     /gene="rplI"
                     /locus_tag="ACG8OL_001124"
     CDS             30407..30859
                     /gene="rplI"
                     /locus_tag="ACG8OL_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS4817126.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31133..32500
                     /gene="dnaB"
                     /locus_tag="ACG8OL_001125"
     CDS             31133..32500
                     /gene="dnaB"
                     /locus_tag="ACG8OL_001125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS4817127.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33280..34572
                     /locus_tag="ACG8OL_001126"
     CDS             33280..34572
                     /locus_tag="ACG8OL_001126"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS4817128.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10581 a         6753 c         6872 g        10557 t
ORIGIN      
        1 tcttgaattg aatcaagacg ccctacacat ttggtttgtc ttactttgtt cagttttcaa
       61 aggtctactt catcaggagt gtttcccgat tcgtttttgg ctgccgtagc aacttttata
      121 tcatatcaga gagacaagta attgtcaact cttttttaaa agttttttcg atttcgtttt
      181 tcgaaatctt ccagcaactc atttatcata acttatcgtt tgttatttgt caagaacttt
      241 tttgaagttt ttttcgatag aatcaagctt cgcaagtggt tcttttttaa cgactcagtt
      301 atcttatcat gtcatttcca acatgtcaac aacttatttt ttattattta ataagttatt
      361 ggtgttcttt tgaacaaata ataatatatc aacatcacat gtgaaagtca ataagaaaat
      421 gcttatttat caacatttct agcttacatg atcattggtt agttctggtc ctttttcgtt
      481 cgtctttata actccgagag acgtaaaatc cttttgtttt cagtttaatg ttcatgttct
      541 tgtcttattc acaccaacta gatgtctcac cttctaaaat tttccttaac aaagtgctgc
      601 ttttatctat ttctgctttt ttttgctaga ataatagtaa caatatgact ctggaggatt
      661 tatttatgaa aattttgatt gctgacgatg ataaagaaat cgttgaatta ttaagtatct
      721 atgttcacaa tgaggggtat gaagcagtaa aggcttatga tgggaaagaa gcattatcta
      781 aaatcagaac aatccctgat attgaattac ttattttaga tatcatgatg cctgagatgg
      841 atgggatgca agtagtgaaa gagctacgaa aagaatccca aataccaatc attatgctga
      901 cagcaaaaac tactgatatg gataagatca aaggactggt cgctggtgca gatgattatg
      961 tgaccaagcc atttaatccg ttagaagtaa tggctagagt aaagtcttta ttacggcgta
     1021 caaaaatgca gattgcccca gatcagcctg atgtattaga agtaaatgca ttaatcatca
     1081 ataaagactc tcatgaagtt aaaacaatcg atgggaaaga aatccagtta acagctttgg
     1141 aattcggtat cctctacttg cttgctagtc atcctaatcg agtatttagc gcggatgaaa
     1201 tttttgagcg tgtttggaaa caagaaagta ttgtctctgc taaaactgtc atggttcacg
     1261 ttagtcattt aagagataag attgaagaag caactggtgg tgaaaaagtc attcaaaccg
     1321 tttggggagt gggctataaa attgacgcaa gatagtaaac gagcaactga aaaacgccgt
     1381 cggatcaatt tgacttcaaa agaaatcagc gaattgttag cggaaggaat catcacgatt
     1441 atccttttgc ttctattaaa tgtttctatt ctggttgtta tcagttcggt gatcaacagt
     1501 tcaccttctt tgacgaatgc gatttgggat tcaaagaata tttttgcaga acgattgaac
     1561 actgatttgt tctggaacgg ccggaatttt attattccct ttttctttct attagatatt
     1621 ggtgttttgt attggcgctt gattcgaaga tatcgccaaa tgcaattgcg gcatatcatc
     1681 agcgaacttc actacatcgc aaacggaaat tatgatcatc gtatcccttt tgaactgagt
     1741 ggtgacttaa gccgagtggt aacaagtatc aatggattgg tggatagtac cgtagcagcg
     1801 attgaagacg aacgtaaaat cgaaaaatca aaagatgaat tgatcacgaa tgtcagtcat
     1861 gatatccgta ccccactcac gtccatcatt ggatacctag gactgataga agatggccag
     1921 tatcactcag aagaagatct tttaaagtat acgcatactg cttatatcaa agcaaagcag
     1981 atgaaatctt tagtggacga cttatttgaa tacactaagg tacgccagcc tgctgtcccg
     2041 gtgaattttt ctgcctttga catgatccag ttgatcgaac agttagcagc tgattttgag
     2101 ttggaagctt ctaaaaaaaa tatccaaata cttgttcaat caaaagtaga ctcccttatc
     2161 atggatggcg ataccgaaaa acttgtacgt gtcttcaata atcttttgac gaatgcgttg
     2221 aaatacggaa aaggagcaac gaagatcgta atcgaagtag aacgtatcgg ttcagaagtt
     2281 gttgcaacag tcaaaaataa cggagcaatg atcccacaac aggcaatcga taatctattc
     2341 gaccgttttt atcgtgtaga agaatctcgt tcacaagcaa ctggaggaac tggacttggt
     2401 ttagcaatcg ctcaaagcat tgtggctttg cacggcggat acatttatgc aaaatcagat
     2461 aagcaatgga cttctttcat catgcacctt ccaatcaaga aaaatgaaaa attacctatt
     2521 gatacacagg aagttattga cgaaagctga aatattcgct attcttatta caattattag
     2581 aataaatgaa aaacacagaa cccagtacac tttattcttt ttcagagagc tgatggcagg
     2641 tgtaaatcag caaaggataa tttggaagtt ccagttcgtt tacgtgccct ttcgaaataa
     2701 gaaaggtcgg ttcaacagcc gttatgtgtt caacaagtgc agctgacgct gaacttgggt
     2761 ggtaccgcga atttcagact gattcgtccc aagaataatg ggatggatca gtcttttttt
     2821 attttcaaaa aggaggaaat catatgttag atgtaaaaat gatgcgacaa aatattgaag
     2881 aagtacaaga aaaattgatg actcggggcg taaaaaaaga aacattagct gaattcatgg
     2941 aactagatga aaatcgtcgt aagctactag tcaaaagtga agaattaaaa aaatatcgaa
     3001 atgatgtttc tgctgagatc gcccaattga agcgtgcaaa agaagatgct tctgctaaaa
     3061 tcgctgaaat gaaagaagtc ggtggcaaca tcaaagcatt ggatactgaa attgctgaag
     3121 tggatgaaaa attgaaacaa attgcaacta ctttgccaaa ccttccccat gattctgtgc
     3181 cagttggaac agatgaagag gacaatgtag aaatccgtcg ttggagcgaa ccacgtacat
     3241 ttgcttttga accaaaacca cactgggaaa ttgcagaaaa tcttgatatt ttagattttg
     3301 aacgtggcgc aaaagtagcc ggtagccgtt ttgtctatta caaaggatta ggtgctcgat
     3361 tagaacgtgc tgtctacaac ttcatgcttg atcagcatat ttacgaacac ggctatactg
     3421 aaatgatcac tccttatatc gtcaacagta aagcgatgtt tggtacggga caatttccaa
     3481 aattcaaaga agatgttttc caattggaga atactgatct aacattaatc ccaacagctg
     3541 aagtaccatt aactaactat tataatgatg aaattttaga tggtgcagat ttacctattt
     3601 atttcactgc actaagccct gcttttcgtt ctgaagccgg aagtgctggt cgggatacac
     3661 gaggactgat tcgtcttcac caattcaata aagtagaaat ggtcaaattt tcggatgtag
     3721 agcattctta cgaagaattg gaaaaaatga cagctgatgc agaagatatc ttgaaaaaat
     3781 tgcatttgcc ttatcgtgtg atggccttat caactggaga catgggcttt tctgcagcta
     3841 aaacttatga cttagaagta tggatacctg cacaagatac ttatcgtgaa atcagttctt
     3901 gttctaactg tgaagatttc caagcacgcc gcgcaatgat ccgttatcgc gataatgatg
     3961 gaaaaatcca ctatactcat actctgaatg gttcaggact tgcagttggc cgtacagtag
     4021 ctgccatttt ggaaaactac caaaatgaag atggttctgt cacagttcca gaagcgctcg
     4081 ttccttatat ggggaatctg aaagttatta aataaagagg ctgtgaaaaa gccgcttagt
     4141 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaattggact
     4201 taataccgag aaactggctt ttgaacacca tttatcgaga ggttgtgaaa tcagcgaatt
     4261 aacctgagta ttagagaaaa aaatggaaaa atagtccctc atattttacc gtttgttgaa
     4321 ctaataccga gggtcagctg attgaacacc gtttagtgga gaggtcgtaa gcctaccccc
     4381 cagctcctag ttacaaggac aaattcaaaa aacagctctt catattttat gaatgttgta
     4441 cttgtgattc ggaggtggca agggaacact gtttattcaa aaagaggctg ggacagaagc
     4501 gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
     4561 tcttcttatt tccgaaggag ttgcttctac ttccgccgtt tatacgtgtt tagagcgtga
     4621 gacaaaagtg ttctttactt ttgtcccacg ctctttaatg tttttaagat tcaactgaat
     4681 agtttggtgc ttcttttgtg atttgtacat catgagggtg tgattctttt aatccactgc
     4741 cgctcatttg gataaattgc gcttcatcac gtaattgctg tagattggct gccccaacat
     4801 aacccattcc tgaacgcaaa ccgccgatca tttggaaaat aatatctgca acacttcctt
     4861 tataagcaac ccgaccttcg attccttctg gtactaattt atttgcttca ttgacactac
     4921 cttggaagta acggtctttt gaaccttttt ccatcgcgcc taatgagccc atacctcggt
     4981 atgttttgaa tcgacgtcct tggaagattt cgaattcacc tggtgattca tctgtccctg
     5041 ccaacatact tcctagcatc actgcatgtc cgccggcagc taatgctttg acgatgtcac
     5101 cagaatattt gatccctccg tctgcaataa tcgctttgcc atattgacga gcaactgatg
     5161 ctgcatcata aatagctgtg agctgaggta cacctacacc ggctaccaca cgtgtcgtac
     5221 aaatcgatcc tggtccgata ccaactttta cgacatctac acctgcatca tataaagctt
     5281 ttgtcgcttc agccgtcgca acgtttcctg caatcaatgt tgcatctgca aaagttgaac
     5341 ggatttcttg aatttttcgg atcacacctg cactatgtcc atgagctgta tcgatgatga
     5401 ttgcatctgc ccctgcttct aataaagcat tcgcacgctc aaatgtatcg cttgttacac
     5461 caacggctgc tgctacaagc aatcgaccat gttcatcttt tgctgcattt ggaaattcga
     5521 tcactttctc gatgtctttg atcgtgatca atccgcttaa acgaccttcg ttatctacga
     5581 ttggtaattt ttcgatttta tgtttttgca aaatcttttc tgcatctttt agagaggtac
     5641 ctactggggc agtcactaag ttgtcttttg tcatcacatc agcaatcggc atgtgataat
     5701 ctgttacaaa acgcatgtcg cggtttgtaa ttatccctac caattttctg ttttcaagtg
     5761 tttcaacgat tggcacacca ctgatacgat atttgctcat caattcttct gcgtctgcta
     5821 caagatgctg cggcgttaag aagaacggat caatgattac gccactttca gaacgtttga
     5881 cttttcttac ttcatccgct tgctggctga tcgtcatgtt tttatggatc acacctagtc
     5941 cgccttgtct tgccatagca atggccatct tgctgtcggt aacagtatcc atacttgcac
     6001 tgatgattgg aatattcaac ttaatatttt tagctaattg tacgctcata tccacttcgt
     6061 ttggtaacac gtgactttct gctggtatca ataagacatc atcaaaagta aatccttttt
     6121 tcgtgaactt tgtttcccag ttggacattt ttagtaccac tcctctaatt tagtttatct
     6181 attaaaataa ttgatttttt catttaagtc aatgatctac ttggaatcaa gttagctttc
     6241 tcatagaata ccaatataaa gcgtttacat ctgtgtgtat taaaagagat tgtaaaaaag
     6301 ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg tttttgaatt
     6361 ggttgggttt attaccgcag atgcaaactt ttgaacaccg tttagtggag aggctgtgaa
     6421 aaagctgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc cacatttttg
     6481 cataaattgg acttaatacc gagaaactgg cttttgaaca ccgtttatcg taaaaaggga
     6541 ctgtgacaag acttttgtca cagtccctta ctattattta actatggata aacaaaatga
     6601 ggttctttac tacccctaga tatctagcaa aatagctgat ctttttgcta gtttatcttc
     6661 ttgggacgga taaacttcca ataatcccgt attttttctt caagtagtaa cggattgcta
     6721 aagcagttgc cccgataatg ataactacta ctggatctaa tactggattg ataactggtg
     6781 gcaatagtgt tgtcgcagaa aacagtacga cccaaagaag tgtcgttcca cctaggataa
     6841 gcaatgtctt acccattcct ggtttcttgc ttcgatctgc tccgggatat tcatattgat
     6901 agatgtattt gtacatcata taaaagaccc agccaccgat tgcagaacct agaacgagtg
     6961 tagaaaagcc ataagctggc gttgttccac gtgaaaacaa tccggtaatc gcaatcatga
     7021 tacttaatag accaagcaac agcaacgtat tatctaacca catcaataaa ggggtggatg
     7081 aattttttac ttctgctggc ttattgatga ttgcttccgt tctttcagag actgtcccaa
     7141 acaattgacg ggctgttctt ccgccttttt gttctgcaac taaagtaggc agaatgtcat
     7201 gaagggcttt tactttttct tcttcagaca aattcgctgc ttctaaagat tttttcaaat
     7261 caaaaatgta ctgttgattt cttttggtta attttgtttc taaactattg ttttctttta
     7321 cgattgctcg taattgttct ggttccatta tctaatagtc ctccttagcc gttccttttc
     7381 tcaacggtcg tcacaaaaga cattctacca tattctacag ctagtttgat agccctttta
     7441 cacattaaag cggaataaca ttacgtcgcc gtcttgtacg acataatctt ttccttctaa
     7501 tcgaacacgt ccagcttctt ttgctgcatg catgtttccg taatgatcca agtcttcaaa
     7561 agagacagtt tctgcacgga taaatccgcg ttcgaaatct gaatggatga ttcctgctgc
     7621 ttgtggtgct ttgattcctt ttttgaatgt ccaagcacgt acttcttgtt ccccagcagt
     7681 aaagtaagta gctaagccta acaaatcata agcagcacgg atcaactgat ccaaacctga
     7741 ttcttcgatt cctaaagctt ctaaaaattc cgctttgtct tcttcatcta attctgcgat
     7801 ttcttcttct gcacgagcac tgaccacgat cacttctgca ttttcttctg cagcaaagtt
     7861 gcgcacttct tgtacataat gattcgcgtc tgcatctgct acgtcatctt ctgagacatt
     7921 ggctacataa agaattggtt ttgtcgtcaa taaaaatagt gatttaacga ttttttgttc
     7981 ttcttcagta aattcgatcg ttcgggcaga ttttccttct tccagtacag gtttgatttt
     8041 atccagtaca gctagttctg ccactgcatc tttatctttg gttttagcaa cttttgctac
     8101 acgagtatag cgtttgttga tagattctaa gtctgctaac actagttcca agttgatcgt
     8161 atcaatatct tctagtggat caacacgtcc ttctacgtga gtaatattat catcatcaaa
     8221 acagcgaaca acgtgacaaa tagcgtctac ttggcggata tggcttaaga attgatttcc
     8281 caaaccttct cctttacttg cgccttttac gattccagca atatctgtaa attcaaaagt
     8341 cgtcggaaca gtttttttcg gatgtacaag ttctgttaag cgttgcaaac gccagtcagg
     8401 tacttctacc atccctacgt tggggtcgat cgtcgcaaag ggatagtttg ctgcttctgc
     8461 tcctgctttt gtgatcgcat taaacaaagt tgattttcct acgttgggca acccaacaat
     8521 tccagcagtc aatgccataa atttattcac tctttctctg catttttttc aattactttt
     8581 tttaatttct tttcaaaatc tcgacgggtc atcatgacga tatgtccgca attcatacat
     8641 ctgattttga tatctgcgcc cattcgaatg acttcccagc gattcgtctg gcaagcgtga
     8701 ggttttttca tttcaacgat atcgtgcaag tcgtacataa aaatagcctc ctttttgttt
     8761 attcttcatc aaattgaata ttcaagatat caagaattcg agtcaaatct ttttgagaaa
     8821 gatattcaat ctcgatctta cctttcccat ttttttcttg gatagctaca cttgtaccaa
     8881 atttatccat caaacgatct tcgctttctc ggatataata tggtttttcc tttgctagcc
     8941 ttgggatttt tttcttttct tttccatcat tttgatttaa ctcagaaaca agctgttcca
     9001 gttggcggac agttaaattt tcagttactg cacgattggc taatttcaag atcaatgttt
     9061 tattttttaa acctaacagc gtacgggctt gtcccataga taaacgctga tcttgtacca
     9121 tttccttgac aagatccggt aaagaaagca agcgcaaata attggcgata taaggtctgc
     9181 tctttcctaa acgactagct acttctgctt gtgttagttt caggtttttc atcagcatct
     9241 catatgcttc tgcttcttct aacggattca gatcttctcg ctgcaagttt tccagcactg
     9301 ctacttgcat catcgcttct tcgtcaaagt ctctaacgat agcagggatc gtttcttttt
     9361 cagctagttt ggacgctcga aaacgtcgct cccctgcaat aatctcatat ccttttactg
     9421 cggaacggcg tacgatgatg ggttgaaaga cacctgattg ttcaatagaa ttagctagtt
     9481 cctgcaaaga tacttcttca aatgttttac ggggctgata cggatttggt cgcagctcat
     9541 tcaatggtag ctggacaacg gtatcatctt ttacatccac cgtctctaag ttttctaaat
     9601 cttgaaacag tgcatcgatt cctcggccta atcctttact tttcttcacg acttaccact
     9661 tcctttgcca atgcttggta gacttcagct cctcgagaac gtggatcgta gtcgatgatc
     9721 ggttttccat gactaggtgc ttctgataaa cgaatatttc ttggaatgat cgtttcgtaa
     9781 actttttcgc ggaagtattt acgtacttct tctacgactt catttcccag atttgttcgt
     9841 gcatcataca ttgtcagtaa gactccctcg atctctaatt ctggattaaa gtgtttttgg
     9901 actaaacgaa ccgtattcag taattggctt aatccttcca atgcgtaata ctcacactga
     9961 acaggtatca aaatcgaatc acttgctgta aatgaattga ttgtcagatg gccaagagaa
    10021 ggcgggcaat cgatcaaaat ataatcatac tgactgctta cttctgctaa agatcctttt
    10081 aatcttgatt ctcttgccat cattgacgtc aattcgatct ctgctcctgc taattggaga
    10141 gtcgcaggca caatactcaa attttcgtgt tcagtgatca aagttgcctc atcaattggc
    10201 aattcattga ctagaacatc atagatgtct cttgtcacat caggttttct gatcccgaca
    10261 ccactggtag catttccttg tgcatccata tccactagca atactttttt gccaaggcta
    10321 gcaagacaag cacctagatt caccgtcgtc gttgttttgc cgacgccacc tttttggttc
    10381 gcaacagata ttatttgtgc catctcttct cctcctattt gattggctgc ttattaggca
    10441 gtccgggctt tcttgggtat ttctttgggg ttggtttctt tttttgaata acgatgatat
    10501 gccgttgatc agagacattc ggcaaggtaa ccgcctcttc tttgaggaat ttaccaccta
    10561 ataaagcaat cgcttgtttt gcttctgtca attcctcttc actttttgct gctttcaatg
    10621 cataaaaata gccttcttgt ttagccaagg gcaaacacaa ttccgctaac acattcaaac
    10681 gagcaaccgc acgagcagtg acaaggtcaa atgatgcacg aaagtgagga ttttgcccaa
    10741 atgtttctgc ccggtcatga tataaggaag catcgattcc taataatcgg gttaactcag
    10801 ataagaaggt tattcgttta ttcagagaat caacgatcgt tatctgcagt tctggaaagg
    10861 caatttttaa aggtatgcta ggaaaccctg cccccgaacc aacatcgcat aaagataaag
    10921 gttgcgtaaa gtctacttca aatgctaatg taagtgaatc gtaaaaatgc ttcagatata
    10981 cgtctttttc atccgtaata gctgtaaggt tcatcttttc gttccattcg actaatgtcc
    11041 gataataaat agcgaattga ttcatttgat gatcggtaag ctctatccct ttttctgcca
    11101 aggattggcg aaattcttct ggtatcataa atattccttt cattgtgaaa cagcaattgt
    11161 ttcacagatt ctctccctac tttaacgcaa aatctctaga catgcaatcc ttccatatgg
    11221 attcttaaaa atttgaaaag taagaaaagc cgatagactt tttctttcta gtatgactca
    11281 taaaatatac acagaagaga gaactagctt tgagcaattt ctgcaaaaac tagttctctc
    11341 ttctatttat tattacgtat tacgctggtt cactgacttt cgcaatcttc ccttgttcga
    11401 tatagaccat taggatactt aggtctgcag ggtttacgcc actgattcgg ctagcttggg
    11461 caattgtttc tggttggatt ttctgcaatt tttgttttgc ttctgttgct aatccgttga
    11521 ttgctgcgta gtcgatgttt tctggaatct ttttcgcttc catgcgtttt aatttttcta
    11581 ctttttccat ggcttttttg atgtagcctt catatttgat ttggatttct acttgttcga
    11641 tttcttttcg tgtcaattct tcattttctg gaataaaccg tagaagttct gcataagtaa
    11701 tctcaggacg tcttaaaaag tcgcttgcta gtacaccatc ttttaatggt gccgcattaa
    11761 tcgttgctaa aaagtcttgg acttcttctg ttggtttgat tcgtacattc atcaaacgtt
    11821 tgatttcttg ttcaactgct tcttttttct tcaaataatt cttgtattga tcttctttta
    11881 ctaagcctac acggtgaccc atttctgtta atcgcaaatc tgcattatca tgacgtaaaa
    11941 gcagacggta ttcagcacgt gaagtcagca aacgataagg ttcgttcgtt ccttttgtta
    12001 ccaaatcatc gatcatgaca ccgatgtaac cgtcacttcg tttgatcact aaaggttctt
    12061 ttccctgaac tttcaaagct gcattgatac cggcaatcag cccttgtcca gccgcttctt
    12121 cataaccgct tgtaccattt gtttgtccag ctgtataaag attttcaacg actttagttt
    12181 ctaatgtcgg acggagctgg tgtggctcta ctacgtcata ttcgatagca tatcctgtac
    12241 gcatcatttc tactttttct aatccagcaa tagaatgaag catgttgact tgaacatcct
    12301 ctggtaaaga agtagataaa ccttgaacat agacttcttc cgtatttaag ccttctggtt
    12361 ccaaaaataa ttgatgacgt ggtttgtctg caaaacgcac gatcttatct tcgatggatg
    12421 gacaatagcg agcgcctacc ccttcaacga tccctgtaaa cattggggca cgatgtaagt
    12481 tgtctcgaat aatttggtga gtcgtctcac ctgtataggt cagccagcaa gattcctgat
    12541 ctagtttata ggcactatct ggtgtgctga agctaaaatg attcggctct ttgtcaccag
    12601 gctgctcttc tgtaacagaa tagtcgatcg tacttgattt tactcgaggc ggtgttcctg
    12661 ttttgaaccg ttcgatagca aagccaagtt cttttagatg attcgctagt ccaatagaag
    12721 gttgagagtt attcggacct gatgaatatt tcaattcacc gataatgatt tctccgcgaa
    12781 gtgctgttcc ggctgtaata acaactgctt tggcccggta gctcgctcct gtggacgtca
    12841 ctactccatg acaaacacca tcttcgacga tcagttcttc gacgattcct tgtcttagcg
    12901 tcaaatttgg ttctttttcg atcgtatgtt tcatttctgt tgaataagca tgtttgtctg
    12961 cttgtgctcg caatgctcgg acagctgggc cttttcctgt gttcaacata cgcatttgga
    13021 tataggtttt atcgatattc ttacccattt ctccgcctaa cgcatcgatt tctcgaacga
    13081 caacgccttt agctggtccg cctacagaag gattacatgg catgaatgct accatgtcca
    13141 aatttattgt cagtaataat gtttttgatc ccatacgtgc agcagcaagt gcagcttctg
    13201 aaccggcgtg tcctgcgcca acaacgatca cgtcatacgc atctgcttca taatgttcca
    13261 atcgtttgtt cctcctttta ttttcctaaa caaaattgac tgaataattg agtgattagc
    13321 tcatcttgta cactgtcgcc gacaacttct cctagatagt cccagcaacg cgtcatatct
    13381 atttgaacga gatccacagg cattcctgaa tcaatacctg caatgacttc ttcaagtgag
    13441 agggaagctt tttctaacaa cgcgatatgg cgggtattag aaacataagt agcgtctctt
    13501 tctcctgttt ctccgctgaa gaataaatca gaaatagctg attccaaacg atctagtccg
    13561 tcgtttttag ctacggaaat tgaaaaaact ggttcatttt cgatcaattt cttcaatttt
    13621 tcttgttcca gctgtgctgg caaatctgtt ttatttagaa gaatgatccg tttcaatcca
    13681 cttgtcgctt ccaataattg ttcatcttct gctgtcagtg gttcgctttg gtttagaaca
    13741 agtaagatca agtctgcttc tgctaatgct tttcggcttc tttcgacccc gatccgttcc
    13801 accacatctt ctgtttcccg aattccagct gtatcgatta gcttgagcgg taccccccga
    13861 acattgacat actcttcgat tacatctcga gtcgttcctg caatatccgt aacgattgct
    13921 ttttcttctc ttaacaaatg gtttaacaaa cttgatttgc cgacatttgg ccggccgatg
    13981 attgccgtac ttaacccttc ccgcagaacc ttcccttgct tggatgtagc aagtagcgct
    14041 tggattcttt gttggatcat ctgcgctttt tcaagcaata acttcgtagt caattcttcg
    14101 acatcatcat attctggata atcaatattt acttctactt gtgctaatgt ctctaaaatc
    14161 tcttgtctca atgagcggat caaagcagac agattgccgt ctaactgatt aagagctagt
    14221 cccattgctt tgtctgtttt tgcccgaatc aaatccatca cagcttctgc ctgtgaaagg
    14281 tcaacacgac cattcaagaa tgctcgtttt gtgaattcgc ctggctcagc taatcttgca
    14341 ccttctctta acaatagctg caaaatttga ttgactacaa cgatcccacc gtgacaattg
    14401 atttctacta catcttcccg cgtaaatgtc tttggtgctc tcatcacgga gaccatgact
    14461 tcatcgacaa gctgattgct cttgggatca acgatatgtc cataatggat cgtatgactt
    14521 ggtacttctg ataatcgttt gtttccagat tgataaactt tatctgctag ttgcacagct
    14581 tgatcgccac ttaaacgaac gatactgatt gcaccttctc caggtggggt agaaattgcc
    14641 gcaatcgtat caaattctaa agtaatttca gccatttttt cacttccttt ttcattttgc
    14701 gcacaaaaaa agtgcccact ccacccttta tgcacaataa acggtgaact tcgcactttg
    14761 actgcttgtt cttgattaaa ttctggtcat agattatcac acttgctata aggaatcaag
    14821 tgtttaaaac aattagatga agttgagcct gttctgcttc cattacctta tatttaattg
    14881 tccttggatg tctctttgcg ttctttggtt tattcgaaga gaagatactg tataaacaag
    14941 gctttatctt tcttaaaaaa aacggtacac taaatggtag cgctatctaa actatacact
    15001 tttgaaagga gaaaatatgt gagcccgttc agatctgctg cattggaaga agaaagtgac
    15061 cctcggatca acgatgtcgt tttatctaca gggaatatca accagaaata tatcgtccgc
    15121 gatatcgttt ttgttgtgga atgcttagaa gctgatttgt ttgacccatc ttttcccaaa
    15181 gatgaccttt tcttaacaac gaagaggaaa ttaaagacga aggtacttga atatggtgcg
    15241 aatgcagtaa tcaactgtca ttttgagtat cagcaaaaaa aacgcgacgg aaagtatttt
    15301 ctggaacttt ttgcttatgg aacagtgatc caatttatcc aaacaacgat tggttaaatg
    15361 aaacagacaa aaaaagcgga tcaaaactga agacagtttc gatccgcttt tttctatcaa
    15421 agttagtgac tacttcatcg tatactctga taagaatgat ttctagtaaa atatacttgg
    15481 tttgaaaaac tttgtcaacg tgatttattc aaaaagtaat ggaatcaata atatttttta
    15541 gcaggttcta ctactaaata acgataaggt tcctcacctt ccgaatgtgt ttttacatgt
    15601 tcatctttgc ttaatgcagc atggatttgt ttacgttcaa atgcaggcat tggttctaag
    15661 aaaacaggct gccctgtccg ttttactttt tcagccgtgc gatcagctaa acgctgaatg
    15721 atctcttggc gcttttcacg gtaattgcct acattgacta ctacagacag cttatttctt
    15781 gctaaacgat ggatgaagac ttgtgccaga tattgcaatg cattcagtgt ttttccatgt
    15841 ttcccaatca gtatgccttg cttttgtgtt tctaaatgga aaacaaccat attatttgat
    15901 tctctttcca ttttgacaag agctggagca tcaagctgtt ttgaaatttc agtaagataa
    15961 actgccaatt ccgttaaagc tgcttcatca cctagctcac ttacttcttc tgttttttct
    16021 tcaagaatcg tttcgttgac tacctcgatc tcagctgttg gttcttcttc caaaatttct
    16081 tctacagtct gttctactgt ttcagaaact ttttcgctga tacttggctg gatcgaaagc
    16141 cgtgcattct ttcgacccat tcctaggaaa cctttttttc cttcttccag tatttcgatt
    16201 tcgacttggt ctttggccaa acccaaatca gccagaccta gttggacagc ttcgtctatt
    16261 gttgcagctt cataaatcgg cattagcatt acctccccta ttatttccgt tttttcttag
    16321 gattcattgc tttcttaaga gcacgttctc tttcgcggat tctccgtgct tcttcttcac
    16381 gctcttttcg aattttgaaa gggttgttga tcagcagcgt ttgtacaact tggaaggcat
    16441 tggaaactac ccagtataat gaaagaccgc tggctaaatt gacacccatc actaaaatca
    16501 ttacaggcat cacgaaattc atgatcttca ttgatgcatt cgattcaatt tgactcatgc
    16561 tagataaata cgtacttgca aaagtaaaga ttgctgctaa aatcggcaaa ataaatgttg
    16621 gatccgcgtt ccctaagttc aaccataaga aatgcccttg tttcaactct ggtacacgtg
    16681 aaatagattg ccataacgcc atcaagattg gcatttgtac taataaagga agacaaccag
    16741 catatggatt gacgccattt tctgcatata aacgctgctg ctcttctctt aacttacttt
    16801 gtgtttccgg atctcttgaa ctatattgtt gctgcagtgc ttttaatttc ggttgtaatt
    16861 cttgcgtttt acgcatgctc tttgtttgga aatgcatcaa cggcaatagg atcacgcgga
    16921 tgatcagtgt aaataaaatg atccctatcc ctgcattacc aaatgacaac gccttgatcg
    16981 cctcagcaaa gtaataaaca atataacgat cccaaattcc tgtactctgt gcgctgactt
    17041 ccccagttcc gcaaccggat aagacgatga ccaaactaac gataccggcc atcaaaagta
    17101 aacgtttgta tttcttcact tactcttttc ctctctgtcg attatttttg caagttttaa
    17161 gacgtggact aaattggagc gaacctcatc ataagtcaac cccttcacac ttggtctagc
    17221 aatcacaata aaatccaact ccggatcaat ttcttctttt atagaataaa ctgctgctcg
    17281 gatttttcgt ttgacttcat tgcggtttac cgcgttgccg accttttttc ctacagataa
    17341 accaactcga aaatgaggct gtcccggttt ttccaaacgg taaacgacga atttgcggtt
    17401 agcaaaagaa tctcccgtat ggaacacggc ttgaaactct cgttcttttt ttactcgata
    17461 cgcttttctc attagttttc tttccttttc tatctctcac ttacgctcat aatcatacca
    17521 taattacgat tgtcctttca tcatttccct aaaaaagagt ataaaaaaaa ccactgaaac
    17581 cccagtggcc taagctgaaa gaacttttct tcctttgcga cgacggctag ctaacacacg
    17641 acgtccattt ttagtactca tacgtttacg gaatccgtga actttttgac gtttacgttt
    17701 atttggttgg tatgttcttt tcatatattc cacctccaaa atgttcttat ctattttata
    17761 aacacagaca tactttgata gtataacgac aaagtagtga ttttgtcaat ataaaataga
    17821 agatcgattg ttaaaactta tccacaagat tatttttttc tatgggagtc ctatgtggaa
    17881 aaagttatgc acagggatta tccacagata ataaatggct ggttttacac agaaaacaca
    17941 cgtgtaaaaa aagtaatcca caatcaggtg atttcctgtg tattccgtct cctaactatt
    18001 ggtatatcta tattttttat ccacagatgc aagaggaaat ctattgattt ttacgagttt
    18061 tccacctgtg cattagtggt gtggaaaact tttttttgac aactgtgttt ttctcttttt
    18121 tctttttgtc gattgtgtgg ataattgtaa ttaaaaaatt tttttcttcg gtttctgtgg
    18181 aaaactattg aaaaaaatgc taaattagta ctagttattc actttttaat gaaggaggag
    18241 gccttatatg gtatccctcg atgctttatg gaatgaatta aaagcaacat accaaaagga
    18301 tttatcgcct gctagctata atacatggat cgaaacagcg aaccctcgaa cccttgatca
    18361 aaaccagctc gttgttgagg ttcctagcaa gattcataaa gaatattggg aaaaaaacct
    18421 ggcgacaaaa atcgttgaag tcggttatat gttatcagga aatgagatta tccctcgctt
    18481 tattactggc gaagaagcag agcaagaaga agttatagaa gaaaaaaatc caaaagttgt
    18541 ggcacctagt ccgctaaaaa aagccatgct gaaccctaaa tatacctttg atacgtttgt
    18601 cattggtaaa ggaaaccaga tggctcatgc agcagcgctc gttgtagcag aagatccagg
    18661 ttctatttat aacccgctct tcttttatgg aggcgtggga cttggtaaga ctcacttgat
    18721 gcatgcaatt ggtcatcaaa tgctgcaaag tcagcctaat gccaaagtaa aatatgtaag
    18781 cagtgaaact tttgctaatg atttcatcaa ttcgatccaa aacaaaacag cagaagagtt
    18841 ccgtcaagaa tatcgaaatg ttgatttact gctagtggat gacattcaat tttttgcaga
    18901 aaaagaagct acgcaagaag agtttttcca tactttcaat gccttgtaca atgaagggaa
    18961 acaaatcgta ctaaccagtg atcggttgcc aaacgagatt ccgaaactgc aagaacgcct
    19021 cgtttctcgc tttgcatggg ggctgtctgt tgatattaca ccgccggatc ttgaaacaag
    19081 aacagcgatt ttacgtaaaa aagcggcggc tgaacgtcta gagatcccag atgacacact
    19141 aagctacatc gcaggacaaa ttgattctaa cattcgtgaa ttagaaggtg cgcttgttcg
    19201 agtgcaagct tttgctgcga tgaatagtga agatatttcg actagtctag ctgcagacgc
    19261 actgaaaacg ttaaaatcag gaaaaggaca tcctcaattg tcaatcctgc agatccaaga
    19321 agaagtggca aagtattatc acatccaatt gaaagatatc aaaggaaaga aacgcgtcaa
    19381 atctattgtt gttcctagac aaatcgctat gtatttggca cgtgagctca cagataattc
    19441 tttaccgaaa atcggagctg aattcggtgg caaggatcat acaacggtta tccatgctca
    19501 tgaaaaaatc cagcagctga tggattctag tgtcagtatg caaaatgaag tatcagaaat
    19561 caaaaacttt ttactaaatt aatttgtcga ttctatcaac tgaaaatagc atgtggataa
    19621 aagaaaaaaa cgttcaaaag ttatgcacac tttttccaca gctgtttttt cgtttctctc
    19681 ttacgttttt tgagttttcc acagaatcaa catgccctat tacttttatt attcttttta
    19741 actaataata aataaacaaa cattcaaagg agatacatca tgaaagttac tttaaaccga
    19801 gctagcttta tgcaggaatt gcaaactgtt caacgagcta tttcaagcaa aaccacgatc
    19861 cctattttga caggtgtaaa aatcacactg acacaagaag gtttgacttt gacggggagc
    19921 aacgctgata tatcaattga aacttttttg tctgttgaaa acgaaaaagc aaatatgcaa
    19981 atcgaatcta ctggttccat tgttttacaa gcacgtttct ttagcgaaat cattcggaga
    20041 cttcctgaag aaacatttac tttagaagtt ttagaaaata aacaagtagc gattacttct
    20101 ggaaaagcga attttatcgt aaatggatta gatgcagata actatcctca tcttcctgtt
    20161 gtcgaaagcc ataaccagat gaaattacct gtacacgtat tgactaaact aatcaacgaa
    20221 acagtttttg ctgtctcaca acatgagagt cgtccaatct tgacaggtgt gcatttcatt
    20281 ttatctgata attctttatt ggctgtagct actgattctc accgtctaag tcaacgcgtg
    20341 attccagtag aacaagcggc tgatcatttt gatattgtta ttcctggaaa aagtttgatc
    20401 gaattatctc gctcattaac aaatgaagaa gaaatcgtcg aaatcagcat tatggaaaac
    20461 caagtgttgt tcaaaacaga aacgatgtat ttctattccc gtttgctaga aggaaactat
    20521 cctgatacca atcgtttgat tccttcaagt tttaatacag aggttgaatt ttctgttcct
    20581 agctttttag cggcgatcga acgggcttct ttactttctc atgaaggccg taacaacatc
    20641 gttcgtttat ctattcgtcc agatgcggtt gttttatacg gaaattcgcc agaaatcggg
    20701 aaagtagaag aaagcttgag ttatacagca agcagtggcg atccattaga tatttctttc
    20761 aatccggatt atatgaaagc agcgttgcgt gcatttggag atatgagcat caaagtgaaa
    20821 tttatctctg ctattcgtcc atttacattg gaaccaacgg aagatggcgt tcaatttatt
    20881 cagctgatta ctcctgtacg tacaaattaa caatgatttt cattgttctt gaaaatttaa
    20941 ttgaaaagaa atagaaaaaa taaaatttta aacagaaggc cctaaaatct cttttaaggc
    21001 tttctgttta tttttgttta aatcaccttt tttagattaa atcgtcttat atggaaaaat
    21061 atatggataa atttgtttta ttgcgaaaaa aaaggtataa tagagtatac ttttaataat
    21121 gaaaatgagg gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg
    21181 ggccaaatgt tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg
    21241 gcagaaaaca ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat
    21301 cccggcatga tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac
    21361 gacgataatg aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa
    21421 catcgaattt tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa
    21481 tcttttggaa agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca
    21541 ggaattgatt ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc
    21601 atccactctt ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca
    21661 tatcgaacaa aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc
    21721 agaagatctt tctttagtaa aaggcagccc gcagatcagg cggaagtttt tagatatgga
    21781 attaggccag atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa
    21841 acaaagaaac cagtatttaa agcaattgaa tgaaaaaaaa caaactgatt tgctttattt
    21901 ggatattttg tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca
    21961 gttcgtcaag cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa
    22021 agagcagctg gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga
    22081 gcaaatccaa gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg
    22141 gggaacaacg acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa
    22201 tgtgcagact tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc
    22261 agaaatcgat ttgattaaag aagagacggg ggaatatcca atccttttgt tagatgatgt
    22321 gatgagcgag ctggatgata gtcgtcaact ccatctgcta gaaacaatcg aaggaaaagt
    22381 tcaaacgttc ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc
    22441 tgagattttt tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa
    22501 agaagtttag tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga
    22561 cttgaagccg ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg
    22621 catcaccttg tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca
    22681 acgaaaatcc atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg
    22741 gggatacctg tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact
    22801 gttttacatg ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac
    22861 ggagtaggat cttctgttgt taacgcgctt tctacacagc ttgatgtcaa agtatacaaa
    22921 gaaggaaatg tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt
    22981 atcgaacaaa cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt
    23041 acagagacaa ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc
    23101 ttgaaccgcg ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat
    23161 gaataccatt atgacggcgg gattaaaagt tatgtcgaat acttgaatgc aaacaaaacc
    23221 gtgatttttc cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc
    23281 tctatgcaat atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat
    23341 acgtatgaag gcggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat
    23401 gattatgccc gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac
    23461 gtgcgagaag gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga
    23521 caaacaaaaa caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc
    23581 gaacatttca tgaaattctt gctggaaaac ccaagtgtgg gccgtcaaat tgttgaaaaa
    23641 ggattactag cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt
    23701 aaaggtgctt tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat
    23761 cctgaaaaat gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa
    23821 ggtcgtaatc gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa
    23881 aaagcaagca tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg
    23941 ggtacagggt ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc
    24001 atgactgatg ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat
    24061 cgttacatgc gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac
    24121 ggggtaaaac aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag
    24181 ttgaaacaag tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa
    24241 ggacttggag aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg
    24301 atgatggccc gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg
    24361 atgggggatc gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag
    24421 aacttagata tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat
    24481 ccatgatgtc aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt
    24541 tattgtcgca cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat
    24601 tttatacggc atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag
    24661 aatcgtcgga gatgtcatgg gtaaatacca tcctcatgga gactatgcga tctatgagtc
    24721 aatggtacgg atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa
    24781 ctttggttct gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag
    24841 caaaatcgct acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa
    24901 ctatgatgat acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt
    24961 gaacggaaca acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc
    25021 tgaggtagta gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt
    25081 gatggaagta ttacctggac cagattttcc aacaggtggt ttggttatgg gaaaatcagg
    25141 aatccgaaga gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat
    25201 tactgagatg ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa
    25261 taaagcaaaa ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat
    25321 cacagatttg cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg
    25381 tgacgccagc gcatctgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc
    25441 atttggattc aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca
    25501 aattcttgaa aactatatcg aacatcagcg cgaagtcatc acaagacgga cagtctttga
    25561 aaaagaaaaa gcagaagcaa gggcacacat tttagaaggt ttgcgtattg ctttggatca
    25621 tatcgatgaa atcattgcga ttatccgcgg gtctaaatca gatgacgaag caaaagcgac
    25681 gatgatcgaa cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt
    25741 acgccgattg accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa
    25801 actgattgaa gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa
    25861 gaatgaatta gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt
    25921 cggtgaggtc ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac
    25981 attgacaaac aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag
    26041 gggtggacgt ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt
    26101 ttcttgttct acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc
    26161 aaaaggatac gaaattccag aatacgggcg cacagccaaa ggaataccaa ttatcaattt
    26221 actaggtatc gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga
    26281 agaaggccac tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac
    26341 tgcttttgct aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga
    26401 gctcgtcaat gtgctactga cagatggtca atctaatatc attattggaa cacataacgg
    26461 ctactctgtc actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt
    26521 tagaggaata cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaga
    26581 tcaagaagta ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata
    26641 tccagtgaaa ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg
    26701 tccactagct ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa
    26761 aggtgtcatt atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg
    26821 cgtacgtttg atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc
    26881 agaagctgca gaacaaacag ttgaagaaca aggatcagaa gaatagccat caagactaat
    26941 tgtgtaagct agcaagaatg accgattaaa aaatctggtt attcctgctg gcttttttga
    27001 ttagttgaat ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa
    27061 tagagtgtat aatcagagct tgtgagtagt ctaattaatt agataactct ccttgctctt
    27121 aggaacctaa gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg
    27181 aaatcatgta cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac
    27241 gtttcgatgc aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa
    27301 aacgccgttt cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag
    27361 tatcttctcc atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg
    27421 acgatatcat tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac
    27481 aatagaaaac gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt
    27541 ctgacgaaag atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact
    27601 ttggctgtga atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac
    27661 tgtgtgattt ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt
    27721 ttaggtgtta ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta
    27781 tacgtgacag aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa
    27841 aatcgccaac aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga
    27901 aacaataact ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat
    27961 acttctgacc cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc
    28021 taaacgaatt tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg
    28081 tcgtaaagta gactacattg ctgctaacca tattgaatat atcgattata aagatatcga
    28141 attactaaaa agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac
    28201 aggcgctaaa aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt
    28261 gctacctttc gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat
    28321 cagctattca attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg
    28381 acgaaagtta tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa
    28441 acaatcattt atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg
    28501 tagtcttaat tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat
    28561 ggggtgttcg ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa
    28621 tccagcaggc ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg
    28681 taggagtgat tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat
    28741 tgaaaatcaa agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg
    28801 caaaagaaaa aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg
    28861 atacagtaaa gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc
    28921 atcagacgaa cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca
    28981 ttgataaatt ggatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt
    29041 ctgactggat gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct
    29101 tcgtagcaca gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc
    29161 gctttcgagc agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt
    29221 atggctcaag aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt
    29281 tagtccgtgg aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt
    29341 attatggggg gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga
    29401 gtaccgcttt gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt
    29461 ttccggatat ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc
    29521 agaaaaaagc ttacatcgtt atcaacgaga acgagatcat tccagatgta gaacggtgtc
    29581 ttgaagaaat ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca
    29641 tagagctgaa aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat
    29701 cgatctctca gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc
    29761 gtggggaaga gtttccaagt aaacctctgc tgacttatat cgaatcttca gcttcttcag
    29821 cttcagagct agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc
    29881 gttttgaggc cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc
    29941 gtacaactgc acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat
    30001 ccttagtaca atatttattg agctcggatt tagcgacata cttggagatc agcaatttga
    30061 ttgctggaag cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag
    30121 agtataatag tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca
    30181 atgcagcttt cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta
    30241 ccggcacgat caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa
    30301 acgcagctac tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg
    30361 caatcaaaga aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt
    30421 tttacaagat gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta
    30481 tgcacaaaat tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag
    30541 tcagttagca ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc
    30601 taaaaagtta aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc
    30661 aggggaagac agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa
    30721 caaacaatac aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc
    30781 actaggctac acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt
    30841 tcatgtcgta caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga
    30901 aggaaaaaag acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc
    30961 tgcaaatcga tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag
    31021 aaaacgagta taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact
    31081 tttctctcat tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga
    31141 agtgtggcag gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc
    31201 tgtttttctt gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt
    31261 ttatcgtcgt ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga
    31321 agcgattgac gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc
    31381 cggtggttta agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt
    31441 ctactatgcg aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac
    31501 aggaatcgtt acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc
    31561 cgaacgcagt atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc
    31621 tgaagtactg aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat
    31681 cacaggtcta ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga
    31741 agaactaatc attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat
    31801 tgctcaaaat gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg
    31861 ggcagaatct cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt
    31921 acgtacaggt cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct
    31981 ttctcgagca aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc
    32041 gaaatgtcgg aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt
    32101 gcagttgatt gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg
    32161 ccaactgaaa aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc
    32221 tcgtggagta gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg
    32281 atcaattgaa caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag
    32341 agaaggcgga gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat
    32401 catcgagaag aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata
    32461 caataagttt tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg
    32521 agcaatcccg agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta
    32581 cttaatttaa acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc
    32641 cgaataaacg gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg
    32701 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct
    32761 cttttcctta aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa
    32821 catgagaagc agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca
    32881 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga
    32941 aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt
    33001 cacaacctct gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc
    33061 acaaaaattt gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc
    33121 tgttacaacc tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt
    33181 ttataaaata aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac
    33241 aatggttcag gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta
    33301 ggtacgcaat ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct
    33361 gaagtgattg cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt
    33421 gtgacttata aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg
    33481 atcggaaacg gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat
    33541 gaaaaaggtg tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct
    33601 tatcatatca aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca
    33661 acgattaaag gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt
    33721 gcagacttat tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa
    33781 aaccgtcaat ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa
    33841 gaatattatg aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg
    33901 aatgatgctt tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg
    33961 gatatcgacc agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta
    34021 acaatcggaa gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc
    34081 tatacgtcac gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa
    34141 caaatccgcg aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc
    34201 tggtttgatt ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct
    34261 ttaaactcga tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa
    34321 ttagatggcg aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa
    34381 ccaatctatg aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca
    34441 gaactgccag aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt
    34501 atctcaacat tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg
    34561 gcgcaaatct aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga
    34621 agtgtttatc tatacgataa taataacttg aagaggtagt gaaagagacg tttagcctta
    34681 ttgccgatag gctgtctctt gaacactgtt tattcagaat aagggactgt gacaaaaacc
    34741 gtgtcacagt ccctttttcc gaa
//
LOCUS       DAZUJO010000021        34567 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000021 DAZUJO010000000
VERSION     DAZUJO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.021"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(215..835)
                     /locus_tag="ACG8OL_001127"
     CDS             complement(215..835)
                     /locus_tag="ACG8OL_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4817129.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            complement(825..1754)
                     /locus_tag="ACG8OL_001128"
                     /pseudo
     CDS             complement(825..1754)
                     /locus_tag="ACG8OL_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            complement(1799..2743)
                     /locus_tag="ACG8OL_001129"
     CDS             complement(1799..2743)
                     /locus_tag="ACG8OL_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4817130.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(2789..3568)
                     /locus_tag="ACG8OL_001130"
     CDS             complement(2789..3568)
                     /locus_tag="ACG8OL_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817131.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            complement(3958..6033)
                     /gene="glyS"
                     /locus_tag="ACG8OL_001131"
     CDS             complement(3958..6033)
                     /gene="glyS"
                     /locus_tag="ACG8OL_001131"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS4817132.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(6035..6952)
                     /gene="glyQ"
                     /locus_tag="ACG8OL_001132"
     CDS             complement(6035..6952)
                     /gene="glyQ"
                     /locus_tag="ACG8OL_001132"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS4817133.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(7336..8148)
                     /gene="recO"
                     /locus_tag="ACG8OL_001133"
     CDS             complement(7336..8148)
                     /gene="recO"
                     /locus_tag="ACG8OL_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS4817134.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(8291..9190)
                     /gene="era"
                     /locus_tag="ACG8OL_001134"
     CDS             complement(8291..9190)
                     /gene="era"
                     /locus_tag="ACG8OL_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS4817135.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(9205..9600)
                     /locus_tag="ACG8OL_001135"
     CDS             complement(9205..9600)
                     /locus_tag="ACG8OL_001135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4817136.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(9584..10060)
                     /gene="ybeY"
                     /locus_tag="ACG8OL_001136"
     CDS             complement(9584..10060)
                     /gene="ybeY"
                     /locus_tag="ACG8OL_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS4817137.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(10077..12269)
                     /locus_tag="ACG8OL_001137"
     CDS             complement(10077..12269)
                     /locus_tag="ACG8OL_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS4817138.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(12293..13264)
                     /locus_tag="ACG8OL_001138"
     CDS             complement(12293..13264)
                     /locus_tag="ACG8OL_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS4817139.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            13970..15349
                     /gene="brnQ"
                     /locus_tag="ACG8OL_001139"
     CDS             13970..15349
                     /gene="brnQ"
                     /locus_tag="ACG8OL_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4817140.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(15505..15951)
                     /locus_tag="ACG8OL_001140"
     CDS             complement(15505..15951)
                     /locus_tag="ACG8OL_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS4817141.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(15978..16154)
                     /gene="rpsU"
                     /locus_tag="ACG8OL_001141"
     CDS             complement(15978..16154)
                     /gene="rpsU"
                     /locus_tag="ACG8OL_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS4817142.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(16316..16744)
                     /locus_tag="ACG8OL_001142"
     CDS             complement(16316..16744)
                     /locus_tag="ACG8OL_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4817143.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            16836..17696
                     /locus_tag="ACG8OL_001143"
     CDS             16836..17696
                     /locus_tag="ACG8OL_001143"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS4817144.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(17780..18130)
                     /locus_tag="ACG8OL_001144"
     CDS             complement(17780..18130)
                     /locus_tag="ACG8OL_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS4817145.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(18146..19873)
                     /locus_tag="ACG8OL_001145"
     CDS             complement(18146..19873)
                     /locus_tag="ACG8OL_001145"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS4817146.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(20086..21012)
                     /gene="trxB"
                     /locus_tag="ACG8OL_001146"
     CDS             complement(20086..21012)
                     /gene="trxB"
                     /locus_tag="ACG8OL_001146"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS4817147.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(21157..22590)
                     /locus_tag="ACG8OL_001147"
     CDS             complement(21157..22590)
                     /locus_tag="ACG8OL_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4817148.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(22590..23993)
                     /locus_tag="ACG8OL_001148"
     CDS             complement(22590..23993)
                     /locus_tag="ACG8OL_001148"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS4817149.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            24223..25125
                     /locus_tag="ACG8OL_001149"
     CDS             24223..25125
                     /locus_tag="ACG8OL_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4817150.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(25166..27052)
                     /locus_tag="ACG8OL_001150"
     CDS             complement(25166..27052)
                     /locus_tag="ACG8OL_001150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4817151.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(27149..28000)
                     /gene="licT"
                     /locus_tag="ACG8OL_001151"
     CDS             complement(27149..28000)
                     /gene="licT"
                     /locus_tag="ACG8OL_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS4817152.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(28458..30242)
                     /locus_tag="ACG8OL_001152"
     CDS             complement(28458..30242)
                     /locus_tag="ACG8OL_001152"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817153.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(30242..31993)
                     /locus_tag="ACG8OL_001153"
     CDS             complement(30242..31993)
                     /locus_tag="ACG8OL_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS4817154.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(32135..32359)
                     /locus_tag="ACG8OL_001154"
     CDS             complement(32135..32359)
                     /locus_tag="ACG8OL_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS4817155.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32610..34196
                     /locus_tag="ACG8OL_001155"
     CDS             32610..34196
                     /locus_tag="ACG8OL_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS4817156.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         9653 a         7355 c         6056 g        11503 t
ORIGIN      
        1 tattataata ctttgccata actctctttt ccttatactt ttttataatt ttctcgtatt
       61 gaaacagtat atttaaaact ctacacaaat ttttaaaatc atacttttat tagctatacg
      121 tttttttaac atatgttata taattctaat atactcgctt tgatttatac ctaaatataa
      181 tgcaagtcac aaaacttgca ttaagctgct gtatttaact ccctttaaat ttttctggga
      241 tattgttatt gattgtatta atattctctt tcttgattac ttttttacat gtctgaatta
      301 taataataag atcattaaag aaactaattt tatcaacata ataaacatct aaatcaaact
      361 tttcttccca actaatagca tttcttccat tgacctgagc cagtccagtt aatcctggtc
      421 ttacgttgtg tcttttccgt tgcttctcac tatatagtgg caaatactcc actaataatg
      481 gacgaggtcc tacgatcgac atatcacctt tcaaaatatt ccaaagttcg ggtagctcat
      541 ccagacttgt tgctctaagc attttcccaa atttagttag tcgaatttca tcaggtaaat
      601 attctccatt gtggtctctt tcgtcagtca ttgtacgaaa tttatacatg gaaaaaattt
      661 tttcactttt tcctggtcgt tcttgtttga agataattgg agaaccaagt ttaattctta
      721 ctaaaaaacc tacaattaag attatcggcg ataaaacgat gagagcaatt cctgacaata
      781 ggatatctaa aattcttttc ccaaaaagct gatagaaagt ttttttacgc atcattgatc
      841 actcttttaa tcgtagatac tacatatttt aaatcttcat ctgtcatctt tgtatctgat
      901 ggtaaacata ctcctaagtc aaataaagtt tctgctacat catcgccaat ataatcataa
      961 tttttaaaaa caggttgtaa atgcataggt ttccatactg ggcgggcttc tatattattt
     1021 ttgtctaact catcaataag atgattcgca tttattccct ttattgttat gacacttaac
     1081 caataattcg gttcattcca attattttct ggcatgaatt gtatatttga caatttagac
     1141 aattcttttt tataaaaact atgaatatat cgttttttct caattctttc atttaatact
     1201 ttcaattgtc ctcgaccgat accagcaacg atattgctca ttcgataatt atagccaatt
     1261 tcattgtgtt gataatggcg tgccttctca cgagattgcg tagcccaaaa tctcactttt
     1321 tccacctttt ctttgtcatt ggatacaagc attccgccac ctgaagttgt gattatctta
     1381 tttccattaa aagaataaat tccatagtcg ccaaatgtac cagtatattt tcctttatat
     1441 gttgttccta atgattctgc agcatcttca attagagtaa catcgtattc tttgcatatt
     1501 ttagtaattt cgtctaaatt agctgataaa ccgtacaaat gtactactaa tacagccttt
     1561 gtgttaggat atttttctag tgctgttcgc aaatgttcag gtttcatatt ccaagtttcg
     1621 taatcactat caacaaatac aggtatagct ccttgataga gaattggatt agcagtagct
     1681 gagaacgtca aggactgaca aatcactatg tcatctttac cgaccccagc cgctttcaaa
     1741 gctaaatgta tagcatttct ttcaagctga ttgtctcttc cattttttct ccactttcct
     1801 atttattctc tagattcatt tgatctttta attctttttt cacacgatca agttcttgat
     1861 cgctgacttt ttgataggag atattatctt gcatgaagcc ttctccttgc agctgatctt
     1921 gcttgatcgt accaaatgcc tcccgataat ctaaagcgat ttttttcatt tcatcaaagc
     1981 tcatgtctgt tttcatattc gactctagag cattcaaaac ggattgatag ttgctgacac
     2041 cgtttaaact caatactttt tttgctacac cttctacgat ctttctttga cgctcttgtc
     2101 taccatagtc accatttgga tcttcataac gcatacgaga ataagataat gcctgatccc
     2161 cgttcaatgt aattttaccg attggaaagt catagccatc ttgtgagaaa gtgaggtcat
     2221 tattgacttc gataccacca actgcatcaa cgagttcctt tagccctttc atattgatag
     2281 agacatagtg atcgattggg atgtctacat atttttgtag ggtatccatc gccatggcag
     2341 ctccgccgaa tgcgtaagcg tggttgatct tgtcttttgt gtcacgacct acgatgtcga
     2401 catacgtatc tcgaggaatg ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg
     2461 caaccatcat cgtatctgat cttccttgat cgacccgacc taaatctcca gtatcaatgc
     2521 ccatcaatag aacagagaaa gattcttgtt tgtccagatc aactggtttc gttcgttctc
     2581 cttgggttcg ttccactgat tcgtaagttt tctcaataga atgtgagaga tccagatata
     2641 attttgctcc tactcctaca attgctaata ctaatacgat aagaactgct aaaataatca
     2701 gaaatatttt tttccctctt gattttcttc ttcgtcttgc catagtttcc tccgtttgct
     2761 aaccttgctt gaatcaacgt tttaactgtc aataacttat attgctatag cccatttgct
     2821 gagcaacagt ttgtgctttt gttgcgtatg ctgcgcttat tttttcattc cggatctgct
     2881 ctatttgact gagcgccgtc aaatattgat cgtaagtagc ggtggcagtc acttgaccat
     2941 ccttcaacat cgtatcgaat aactgatcta gttgcgtgac ttgcgcgatc tgagtatcag
     3001 cgaatcctag atattgcata acaaccgttt tccaaggact atctttaaaa aagctcatat
     3061 tttcacgtac acgagcgaca tctgcacttg tcaccttttc tttgatgacg acatcgtcta
     3121 cagcttgctg ccagtttgat acatttttag taaataaatc gttgacagct gtctggatct
     3181 tcagttttga ttctgcacta tctaactgtg tcacgattgc tttcttctca tcttggattg
     3241 caacagcttc tttaggcaat tctgattctt tgatgtcgta atcatctgct gaaagtttga
     3301 cagaggatag cttatttgac aaacttgtga ttgtttcttc tgttatttca gggtttagaa
     3361 aatttttttc ttttgtacta tacagcttgt ctacttcttt tgataaagaa gtcaaggtgt
     3421 ctttttgatt ggtgatggca gattctgcat aattgatccg ttgctgatgt tgccgctgat
     3481 tattgatatg gtaaccaaac aaacagatcg caatagcacc tgcacctacc gccacagcag
     3541 tcaatgactt gacgaatggc ttttccatag attcactcct aagtattgat gatagataaa
     3601 ttaagatata attcacatac ataaaaatga cagcttttta ttttaaaaaa cataattaaa
     3661 ttaatatatt cacagaaata tttgtaaaca agttttccct atataatcat accacaaact
     3721 attttcttga ctagggcaat ttacgattta aaagtaaata aaacattaat aagataacaa
     3781 ttaatcgatt tcatttttat ttccttaatt ttcataggtg tttttaaaag ttacacaaaa
     3841 aataaaataa caaaaaaatt aacattgaat taataatagt tatttatatt tataacaaat
     3901 aaaccagaga aatagcctat tttgtgcctg ttctctggtt tatttacata ttagaaatta
     3961 ttttgtgatg atttgatcta aacttgctag tgacaaagcc atggcagcaa tttgacttaa
     4021 ttgcgttaat cggttcttac gtactgctgg ttcttctgcc atgaccatcg tctgatcaaa
     4081 ataattttcg atcagtgggc gaaggcttac aagctgatca taattttcgt tgatcgtttg
     4141 gtcagcaaag cttgttttca ttgcctctac tgctttgtgc agttctttct ccgcttcgtt
     4201 ttcgaacagt tcagggtcta cttctgattg tttttcaagc tccgcttttt tcgctaagtt
     4261 cacgacacgt gtcaaagctt ccatcgatgg tttgaagtct tcatctgcaa gatgctgctt
     4321 gaacaattgt gctgctgcaa atacttttgt cagatctttt tgttctgcat tcaaaacagc
     4381 ttcgatgaca tcatgacgga tattcttcgt tgtcaataat tggcgcagac ggcctttgat
     4441 aaagtcgatc acctgttgtt gtcccgctga caagccaatg ccaaaacgat ccgtatcttt
     4501 actgataact tcactaatcg tttcttgcag aacagacaaa ggaaaggccc attttttcga
     4561 ttcgatgatt cgaacgacac catatgcttg acggcgcaaa gcatatggat cgtttgagcc
     4621 acttgggatc atcccaactg caaagaaaga aaagatacta tctaatttat cagcaatcgc
     4681 taaaacagaa cctactgtag tctcaggtaa atctccttca ctggatatag gcatgtaatg
     4741 ttcacggatc gctgttgcta cggctggttt ctcgcctttc agcagagcat atttttctcc
     4801 catgatccct tgtagttcag ggaattctcc taccatgttg gttactagat caaatttata
     4861 gatttcagca gcgcgttgca gatccatcaa ttcttcctca gatagtccta cttgttcgcc
     4921 tatgatgcga gcgatgactt gtacccgttg cattttctca tagatgctac cgattttttc
     4981 atggaaagta acagttttta atttttcaac gtattggtcg attgaagctt tcttgtcttc
     5041 attataaaag aattccgcat cttctaaacg agccgtcaat actttttgat tgcctcgtac
     5101 cacattttcc aattttaccc gatcgccatt acgagcagaa ataaaatgag gaagcaacat
     5161 accttgatcg ttccgtactt cgaaataacg ttgatgttct ttcatagaag tgactagtac
     5221 ttcttcagga acagatagat atttttcttc gaagtcgcca gaaaagacag ttggatattc
     5281 gactaaattg ttgacttctt ctagtaaatc ttcatctaga tcaatgatcc agtgattttt
     5341 ttcggctaat tcgtttgctt gtgtgatgat catttctttt cttttttgcg gtgaagcaat
     5401 aacaaattgt tcattcaatt tttcttcgta ttcttttgca tgcgtaaatg tcacatcttc
     5461 gcctaagaaa cgatgacctc ttgaagctct gtctgttttg acatccaaga tctcaaacgg
     5521 gattatttcg tcatctaaca atgccacgat ccagtgaata gggcgaatat attcaaaatc
     5581 gtattctgcc cagtgcatcg ttactgggaa agtgaggctt ttaatgacat ctgctaaacc
     5641 tgtcaatact tcttttgctt ctttgccgtg agtgtatttc gttacataga catattccac
     5701 gccttttaat tctctaaaag tgatagcatc tgtcgtcaga ccttgtccgc ggacaaatcc
     5761 ttcagctgct ttcgtccagt ttccatctgc gtccaaagca atttttttgg ctggtccttt
     5821 gacttcttct tcgctgtctg cttgacgttc tggaatgttt gttactttga ttgctaagcg
     5881 gcgcggtgta gaaaacgttt cgatcgtatc ataagtcaat tggttttcat ctaaaaattt
     5941 tgctgttttt tcttctaatt gtttcatgct tggtgtgaca acatgagcag gcatttcttc
     6001 cagcccgatt tcaagtaata agtcttttgc catcttattc agcctccttc atgttttctg
     6061 gatttgctgt gtttttattc aataaaggga atcctaattt ttcgcgttct gccacaaaga
     6121 ttttggctac agcacgagcc atgttgcgga tacgggaaag ataacctgct cgttctgtta
     6181 cggatactgc accgcgagca tctaataaat tgaatgtatg gctgcatttc aaaatataat
     6241 catacgctgg atgaacaaga ttttcatcga tacagcgttt agcttctttt tcaaaacggt
     6301 caaagttgct tagtagcaag tcttggtcac ttacttcaaa agaatatttg gaatgttcat
     6361 attctggttg tttgaagatc tcgccatatt tcacaccatc agcccattcc agatcataga
     6421 cactttcaac ttcttgaata tatgaagcaa gacgttctag tccataagtg atctcagaag
     6481 taactggttt acaaggtagt cctccgactt gctggaagta cgtgaattgt gtgatctcca
     6541 tcccatcaag ccatacttcc caacccacac cggcacaccc catcgatggg ttctcccagt
     6601 tgtcttcaac aaaacgaata tcgtgttcta atggatcaat tcctaaaaga cgcaagcttt
     6661 ctaaataaag ctcttggata ttttcaggtg aaggtttcat tacgacttga aattgatgat
     6721 gctgatacaa acggtttggg ttttctccat aacgcccgtc agctggtcgg cgggatggct
     6781 ctacataagc agcattccat ggttccggtc cgattgcccg taagaatgta tatgggctca
     6841 tcgtacctgc tcctttttct gtgtcataag cttgcatcag catgcagccg tttgatgacc
     6901 aaaatttttg taatgttaaa atcatatctt ggacggtcaa ttttttactc atctgttttg
     6961 cccctttcaa tttttaagcg ttgctgtcaa taagaataca ggaaagaaaa cgacaaaaag
     7021 tccctatgtc tgattacaga catagggacg aataatttcg cggttccacc ctacttccga
     7081 ataaagattc ggcagcttca tttatcgtac tcgcaagtgc ctcttcaaaa atcgtttggg
     7141 tccggcttcc actctcccga actcgctaaa ccacgtgttt tttactgatc ttggtcaatg
     7201 tacgtattca ttttgcagtt ttcttgttta tataatagat cctctcagtg aacgatctat
     7261 ttaatctacg gttaataata gacggaagtc cctgtatttg tcaaatagtt ttgatttttt
     7321 tgttgtatct ttcatttatt cttctttttc aggtatctct ttttcaggca tatttttctc
     7381 tggcgtcttt aatacgtgtg cccaagactt catttgatca atgaactttt tgctttttag
     7441 atgaataccg acatattcat catacaactg gtcaatgagg tcacggattg cctgcttcgt
     7501 ttcttctttc aagtggattc cactgatctt atcataagaa atagccgaaa acattcgaac
     7561 gaaataaaca gcgcgaggat ctgcatgata acggtgttcg tccattggcc agtgtttctc
     7621 gcatatcaca ccgccagatt tggaagaata gtcaaatttt ccttttgttt ccccgcatac
     7681 gacacagtgc gtccaaattg gcgcgattcc gaagcgttgc agtatttgaa cttcaaagat
     7741 attcgtgatg atttctgcat catctccttg atccatccgc tgcagagctt gttgcgtaaa
     7801 ttggaataaa tgcgggtcat attgatgatc ttcgattgct gcatctacta gatttagcaa
     7861 ataggtacca taaccgctca aaaagatatc ttcttggatg ttccgaaacg catggacgtc
     7921 tttgacactg cttaaaaacg acaaaccttc acttcgaaaa ttaccgatat agactgcttc
     7981 ggtaaagggg agcaaagccg gagctaatgg ctgattttgc cgatggattc ctttgatata
     8041 aaacatcatt ttgccagaag attccgtaaa gattttgact aatttatctt tttctttgta
     8101 attacggcta gataaaatca agcctttcga ttctgcaagc tggctcatat tctccctcct
     8161 aaaaaataaa tctggaaaag cagattctgc cgatccagat tatttatgtt tcgatcttcc
     8221 gccgtttttc tcaacgtgcg gatttttatc cagtttattt ctatacttaa cctataagaa
     8281 gttcagaata tcagtattcg tcttttcggt agccaaagtc ttgcagatag acttttttgt
     8341 ctcgccagtc tttttggact ttgacccaaa gttctagaaa tactttgtct cctaaaagat
     8401 tttcgatatc ctttcttgct ttcgttccta cttgcttcag catttttccg cctttgccga
     8461 taatgattcc tttttggcta tctctttcga cgatgatcgt ggcctggata tggattttat
     8521 cattctcgtt tcgcttcatc gaatcaacaa cgactgcgac agaatgaggc acttcgtctc
     8581 gtgttaaaaa caagactttc tcacggatca attcagaaac gataaagtat tcaggatgat
     8641 cagtcacttg atcatctgga aaatattgtg ggccctctgg catttcactg actaatacat
     8701 ccattaagcg ttcgacattg tttccttctg tagcagagat cggaatcact tgtgcaaaag
     8761 gcatctgcgt cgaataatct tcgatgattc ccagcaactc gtctggatgg atcttatcga
     8821 ttttattgat aatcagataa actggagaat gcacgttttt tagacgttca ataataaaat
     8881 cgtctccccg gccgcgtttt tgatcagcac tgatcataaa taagactgca tccacttctt
     8941 tcaacgcact gtaagctgct tctaccataa aatctcctag acgatgcttt ggtttatgga
     9001 ttcctggtgt atcgataaaa atcagctgag cttctggtgt agtatatact ccttggattt
     9061 tatttctagt tgtctgtgcc ttgtcactca taatggcaat tttttgaccc acgattcggt
     9121 tcaataatgt ggattttcct acattcgggc ggccgacgat tgcgacaaac cctgatttat
     9181 gttctggcat atacgttctc ctttttaatt aaacaacaaa tgatatattt tagggacaaa
     9241 taaaatcaac ccaatgatga cagcaaaaca agcggtcact aaaactgctc cagctgccat
     9301 atctttgatt tttttcccaa tcggatgaaa atgaaaatcc gtaaacatgt ccactacatt
     9361 ttcaaaaaca gtattgatga tctctactat ccaaacgaga aaaacagcta aaagcagcca
     9421 taaccactct atccgatcaa gctggaaaat cacgcccata aataaagcaa ggataccgaa
     9481 cgcaacatgt ttcctcatat tgcgttcttc ctcaaaaacg gttttgactc cttggacagc
     9541 aaattccaca gacatcagga aatgtttgtt tttctctacg tttttatctt ttgagtccat
     9601 aggcatccaa gatctctttc tgtaaaccaa acatttcttt ctcatcttct ggtgtcatat
     9661 ggtcataccc gttgatatgc aagaatccat gtaccgctaa aaagccaagt tcccgatcat
     9721 atgaatggcc gtaagcttct gcttgttcag cagcacgttc aatagaaatc atgatgtctc
     9781 ctagtacacg aggcaaagga ttctctccat cttcaaaaat cactgggatt tcgtcttcgc
     9841 cttcttcttc cagagcaaaa ctgatgacgt ctgtcggtgc atcttttcca cggtaatcac
     9901 gattgattac ttggattgcc gcattgtcca tgaaagtaac agacatttct gtttcctctg
     9961 gcagctttaa ataagatgct gcaaattgca gcaagtcatc gatttctttc attttctctt
    10021 ctgagacttg gtttgtttca tccataaagg taatatccat tatacatcgc ttctttctat
    10081 tcttcttgtt cttcctttat cttctcaact tctgttttca ttttaggata tttgatccga
    10141 gaatggaagg tgctacttaa accgctgatc aatgattttt ccacttttcg gatttctttc
    10201 aaggtcagtc cactatcgtc taattgacca tcggaaatac gctcttcgat aagattatgg
    10261 acaaaacttt cgattttttc gctcgtcgga tgatccattg cccgtactgc agcctcacaa
    10321 ctgtcagcaa tattgacgat ccctgcttca cgactttgcg gttttggtcc aggataacgg
    10381 aattgctctt ctgttacttc cggattgcgt tctttcgctt tgatgtagaa ataacgcatt
    10441 agcgtggttc catgatgctg tttgcaaata tcgatcacca tttgcggcat attataatct
    10501 tccaatattt tggctccatc tgtcacatgt ccaaagataa tatgtttact gtcttctggc
    10561 aataagaaat tgtgcgggtt ttccgctcct gaaggcaagt tctcaacgaa aaagctagca
    10621 tgtttgatct tgccgatatc atgataatag caagctactc gagttagcaa agaacgtccg
    10681 ccgatttctg ctacagcgtt agcacttaga ttagctacca tcatactatg atgataagta
    10741 cctggcgctt cttccagcaa ctgtttcaat aaaggatgat ttggattgct gagttcattt
    10801 aatacaatta cactgtcatc attgaccatc aattcaatat atgggtgtag accgatcgtc
    10861 agcaagaatg agaagagtgc accagtgaac ccgcagacaa gcattaacca agtcgttgca
    10921 tcaccaaagc tcattccttg atagacgatc aaaatgacat ccatcatcac aggaaaaata
    10981 atgacccaca tggcagcaga taatccttgt tctgagatcc gtttgcgtct gacaatcgtt
    11041 gccatgagcc cagagaaaac ataagacatc aagataatcg ttagaaagtt cgtgccaatc
    11101 gaatcataaa aaatgaaaag tgcagatact gcttggaaaa taccagccat gatccctgct
    11161 cttcggttca agaaaaagct taaaacaagt ggtgcaaaag cagcgggata aaacagcggg
    11221 atataagtag cttgttccgt ttggaataat tggaaaaatt tcatcagcac tatgctgact
    11281 gacatagctg tcacatagaa aagcacgtat tttgttcttt ctcctgcatt ttggaattga
    11341 agtgaattat aaatcagtac gccgatttga agcaaaacag ctaaaaccat ggcaatgagt
    11401 gggaaaatcg aagtcgtttg attcgtcaat cctagtaatt ccaatttttt catcgctgaa
    11461 gcatcaattt gatttccttc gcgaacaatg atttctcctt ggaagatcat cactggttgt
    11521 acagcttctc gtgcagattg ttttaattct tctgtctttt tctcattcaa tacatcattg
    11581 actacaatgc cttgatccac taagtagcga atcatttgtt gctgctctgt tgtgacattc
    11641 aaatactgaa tctgatcttc tgcttcttgt ttaaaagctt ccaattcgct ttcacgaact
    11701 tgtttactca tctgttcgtc aatgagcttc aaactttcat ccctaaccgt gtgaatttcc
    11761 gtttcagtca tttcaaatat cgtcgaataa aaattgttcg gcagtttttg gtaaaacgca
    11821 acgttttctg cattcacgtt ttcaaaattt ttcttcagtg tagcaatccg ctcttctgta
    11881 gtaggtttcg ggatattttc atcttccttc gccttatcta ccttgtcatt ataagatttg
    11941 ttgatggtat cattcgtctt atcgatcaat tcgaataatt gcttgatccg attgttctgg
    12001 tcatctgcca gatctttttg ataagtatat tctggagtca ctgcttctgc tgccagtttt
    12061 cttctttgtt cggtttcttc cgtgttctcg atcgttttat tcgcacggat actctcttct
    12121 gctacttggc cttctctgaa atcattggcc ttttgtttga cactactaaa tgtaacgccg
    12181 cagacaatga taaaaaacaa gaacagaagg aaaggcaatg ctgcctttcc gagtttttct
    12241 tgtagtttct gcagtgtttt attcaacatg tgcttatcct cctaagcaaa gcttattctt
    12301 gatgaagatc cgccttttca taagctttga tgatttcggc aactacaggg tgccgaacaa
    12361 cgtcacttgc ttcaaaatta acaaaatcga tttttttgat ttgctttagt gttcgttctg
    12421 catgaacaag accacttgtc gtccctcgag gcaagtctat ctgacttgta tccccattga
    12481 caatcatttt tgattgataa ccgagacggg tcaagaacat tttcatctga gcgactgtcg
    12541 tgttttgtgc ttcgtccaag ataacgaatg catcatccaa tgtccgcccc cgcatgtaag
    12601 ctaatggtgc gatttcgatt acgcctcgtt ccatcaatcg gttcgtatga tccaatccaa
    12661 aaatttggta taaagcatca taaacaggcc gcagataagg atccactttt tctttcagat
    12721 ccccgggtag aaagcctagg ttttcccctg cctctacagc gggccgcgtc aaaatgattt
    12781 tttgcacttc gccttttttt agggcagcaa ttgctaatac tacggcaaga aacgttttcc
    12841 ctgttccggc tggccctaca ccaaatacga tgtcatgttt ggctaccgat tcaacgtatt
    12901 tcttttgacc gctgtttttt acccgtatag gttttccatt acggtctttg acgatctctt
    12961 cctcatacat ctcaacaaaa taatctaatg tacctttttg ccccattttc aatgccgtca
    13021 cgacatctgg tgtacttacg tggattcctc gtttgatcaa ttcctgtaat gtccgaatga
    13081 ctgaggcagc caaggaaatc tgggattgtt caccaatgat ctgaatgatt tctccacgtg
    13141 tatggatggt tgtttccgtt gtttcctcga tcagttttat atgcttgtcg tgtgcaccta
    13201 aaagcatgct gacatcatca gcagctgtca atctaatctc taaagaacta gactcttccg
    13261 tcaaaaatag acatcccctt tttttcgatt cattctttcc tattttacca tactcagaag
    13321 aaactatata gcaaataaga aaaggttgta aaagagtcgt ttaaccatac gtaccaagaa
    13381 acgttaaaaa atagctgttt ccattcgcta aaggtctcgt tttgataaaa agaatctgag
    13441 aatgtaagtt atatgaaagt ggagaacaaa atattccgtt tcattcagag gtattctcta
    13501 ggtatttttc ttgtttcaaa aaaagaaaac aaaatagtta aaatattctt gcatttcaaa
    13561 aaaaacaggt gtattattac gtcaacaaat aaacatacag caaagcgtcg aggaaaacag
    13621 taggctattc aacgatttat tcccagtgag tcatgtaagt gagaaatgac aataaatcga
    13681 atagacgaac atcatttccg agtcaatgtc gaaaacgtag atcattgtgg ctgcctccat
    13741 tatcaggcta gcggataact ttatcaaaaa gcgtccgtga atgaagaagc ttttttaagc
    13801 gaaaaaaggt ggtaccgttc tagaatctag agcccttttc ccaagtggaa aagggctttt
    13861 ttttatattt taaggtggtg gttttcatgg aatctgatat tagtggcatt actaattgct
    13921 gcaacgttta tatcgtcaat caaaatgata attattggag gaaaaattta tggaagaaac
    13981 aaaaacttat tcatggaaac aactattgat ggtcagttca cttatcttcg ggatgttttt
    14041 cgggtcaggt aatctaatct ttcctgtcca cttaggtcaa atggctggcg caaactgggt
    14101 cagtgctggc gtaggatttg ccatctcagg cgctcttttc ccattactag ctatcttagc
    14161 tgtagtagtg acaaaaagtg atggtctgta tgacttagct aaacctgtcg gaaaatggta
    14221 tgctgccttg ttcttaatcc ttgttcattt gacgatcgga ccatttttcg gtacaccacg
    14281 gacagctgct actgcttttg aaatggctgc taaaccattt ttgcctgaaa aatataattc
    14341 attaggtatg tttttattct ctgctctttt ctttttactt gcttatttcc ttactgtcca
    14401 tcccacacgg ttggtcaaat acgtcggaaa gtatctaaac tccttgtttc ttgcattatt
    14461 agcaatcatt tttgtcgttt ctttattcca cccaatggga aatctaaatc aatctgctgc
    14521 agatacttac caaacaaacg ctttaacaaa tggtgtccta gaaggttata acacggttga
    14581 tgcagtagcc ttgcttgcct taagtgtcac ttttgtccat gcagtccgtg accttggttt
    14641 caaagataaa aaagtcacag aattaacagc aaaagctggt agcctgagcg ttgcgctaga
    14701 agtcatgatc tattttggtc ttgttcttct cggtgcgatg agtttgaata agatgtcttt
    14761 atcagaaaat ggcggaactg cactttctca gatcactaat ttttatttag gtaacttcgg
    14821 cgctagcttc ttaggagtga tggtcacatt aggtgtcttc actaccgcta tgggactcgt
    14881 tgtttctttt gcacaagatt tccataaact gttccctaaa gtcagttata tgacatggct
    14941 gcggttaacg actttcgtct cttttgtcgt tgcaaatgct ggtttggaca atatcatcca
    15001 atggtcttta ccagtattga tgcttttgta tccattgtcg cttgcgttga ttttgctttc
    15061 cttaacagca aaatttttcc aaaagacacc atttgtctat caagtaacga tgttgtttgc
    15121 tgctgttccc gccgtattgg atatgctggc aaattcgcct gctttagtga gtcaacaaag
    15181 agttgtagca agcatgctcg aattttatca tcatcacgtt ccttttgctg cattgggact
    15241 tggctggatg gtcccaacgt tacttgggta tgctggcagc ttactcttct attacgccta
    15301 tcgcctatca gggtataaac aagaagcaaa tgaactacca gaagaataac cagatcaaac
    15361 acaatttaac ataagaaata ttaaacagga aaaaaacagc tagatgaaag aatcctattc
    15421 tttcgtctag ctatttttta gatctctttt ttacgagcac aaaaaaactg cggacaatga
    15481 ttgtccgcag tttaaatgat aaaattaaga ttgtaatagt tctttcacaa tggcatttac
    15541 ttggtttcca tctgcttttc ctttgacttt aggcatgatc gcacccatga ctttgccaaa
    15601 ttctttcatt gatgaagcac cagtttgcgt gattgcttct tgaacaagtt gtcgaatttc
    15661 ttcgtcggaa agttgttccg gcatgtattt ttcgactatc acaatttcac tttttacttt
    15721 ttcagctaaa tcgtcacgtc ctgcttcttc aaactcatgc aaagaatcac gtcgctgttt
    15781 catttcgcga gacaacaccg ttaattcttc ttcttctgtc aagtcgtgac cttctttaat
    15841 ctgctcgttt tgtatagatg acttgatcat tcggatgact tgtaaagatt ccttatcttt
    15901 cgctttcatc gctgttttca tgtcatcgtt taatctactt agtagtgaca cactaccaac
    15961 tccaattcat cacagaacta gaattttttg cgttttctag ctgcttcaga ttttttcttg
    16021 cgtcttacac ttggtttttc gtagaattca cgtttacgag attcttgtaa agtacccgct
    16081 tttgacacgg aacgtttgaa gcgacgaaga gcatcgtcaa gtgattcgtt tttgcgaacg
    16141 actgtttttg acatgttaat tccctccctc cgagcttaaa aagtaaccgt aattgttatt
    16201 gaaatatatc tcaaaaaaca agactgtcct gtaaatatta tatctaatgc tgttttgacc
    16261 gtcaaggaaa tcttgctttt ttttaaaatt atcaaatgtt attttattta aatccttatt
    16321 gacaattttc acatcttccg aaaatctcga atctatgccc ttcgatcgta catccaccga
    16381 gctgttcttc gaaataattc attggacaca tatggatttc tttcgttgtt ccgcacaccg
    16441 tacagataaa gtgatggtga tggccgattt ccccttgaca gcaatgaaaa cgaaacttca
    16501 tctctccatt taaatcggtt tcttccagta aatcgatttc tgcaaaatca tggagatttc
    16561 gataaatcgt atcgtaactt actcctttga attcctgatt catatagtca aaaacttctt
    16621 tagcggatac gtagcgattg cgcttcacta aataagttaa caatgcttct ctttttttag
    16681 tatatttaaa accgttattt ttcaatgttt ctagtgattg ttcgatcaac gtctgttttt
    16741 tcatgactta ttcctccaaa tcaaaatgat tacgaagtac acattctaga gtatggtata
    16801 ataaacataa aaaaaatgca agtgaggttt tatccatgtc ttccgattct acagaaatcg
    16861 ttactttttt tgtcatctcc gactctgctg gagaaactgc gacaaaacta gcgcaagcca
    16921 ctatggccca atatcctagc gttgagttca atttattccg cagaacattt gtgacagatg
    16981 agaaaacatt gatgcaggca ctgcaagatg cactagaaga aaaagctctt gtcctgcaca
    17041 cattgatcaa tcaagaattg atcgagatga cgcgttcttt ttgtgaagaa catcaattat
    17101 ttagttttga tgtgatgact ccgccgatat cagaaatcga gcgcttaact ggtatcaagc
    17161 ccattcggca acctggcgcc cttcatttat tgaatgagaa ctatttcaaa cggatcaaag
    17221 caatggaatt tgctgtaaaa tacgatgacg ggaaagatcc tcgtgggttt ctagaagcag
    17281 atgtcgtttt gctaggcgta tcccgaactt ccaaaacgcc tttgagcttg tttttagcta
    17341 acaaaaattt aaaagttgcc aatctacctt tgatcccaca agcacatatt cccaaacagc
    17401 tttgggagat cgatcctaaa aaaatcgtgg gactgacgaa taacccagat attttaaaca
    17461 atatccgtaa agaacggatg cgctcttacg gattaaatcc tgatactgct tattccgata
    17521 tcgaaaaaat acgagctgaa ctcgattttg cgaatgatct ttatgaaaaa ttgggctgcg
    17581 tagtaatcaa tgtcgcttct ttatctattg aagaaacagc ttcgcttatt ttgaatgctt
    17641 tagacctaga agaccatagt tattatggaa ctgaaaccac cgaagaaaca gactaacaat
    17701 ttttctttaa aaaaactgga ctgtgtcagc gaagaagatt cgttgacaca gtccagtttg
    17761 tctctatttg cacctatcgt tattgatgtt ccacatgttt gagtgtctgg ttcaaaatat
    17821 ccagtacgtg agaatcatca agactataca aaatcgcttt tccctcacga tgggacttga
    17881 cgacatggtt ttctcttaat aaacgcagtt gatgcgatac agcagattgt tccatttcca
    17941 ctgcttggat gattgctgtc acattttgtt ccccgtcttt cagatacagc agaattttga
    18001 gacgagtggg gtcactcaac attttgaata gttggctgac tttctctact tttgtttttt
    18061 gcgtgtctgg caatccattc acctccaact tgaaaaaaga ctgacttcta aagtttacca
    18121 ctttttccat cagtctcaag tttttttatt cttctgtcat cttttggata atcgtttcat
    18181 aagcggctac tttttcagct gcgtcttctg aagagtttcc ttttacaccg atataaaatt
    18241 tgattttcgg ctcagttcct gaaggacgga tagcgatcca gccattttct tccagcgtat
    18301 acttcaatac gtcagatggc ggtgtcgtta gtttttctac gtttccttca cggtctgttc
    18361 tcgtcaactc tttgaagtct tctgtttgga tgacttttgc tccggcaaat tctgttggtg
    18421 cttcttcacg gaatttttcc atcaaagcag agattttcgc agaaccttct tgtccgctca
    18481 ttgtcacaga aatcgttttt tctgcgtagt agccataggt ttcaaaaatc gtttgcaagc
    18541 catcatacaa cgttttgcct tgttttttat agtaagctgc tacttctgct aataagagca
    18601 atgcttggat cgcgtctttg tcgcgaacga aaggtttaac taaataaccg taactttctt
    18661 cgaatccgaa cataaaagta tgggaatgat cttcttcaaa ttgttgaatt ttttcagcaa
    18721 tgaatttgaa tcctgtcaat acattgaaca tggtagcccc atagtgttcc gcaattgctg
    18781 taggtaattc gctagaaacg atcgatttca ataccgctgc gttttcaggt aaagtgcctc
    18841 gcgattggtg tgcttccaaa atatattgag caaacagtgc tccgatttgg tttccggtca
    18901 atacttcata ggagccattt ggcaaacgaa cggctgcacc taaacggtct gcatcaggat
    18961 ctgttgcaat cagcaaatca gcgccttctt tctcgccaag tcgtatcgca tactcaaaag
    19021 cagagtgttc ttctggattt ggtgatttga ctgtagtgaa atctgggtct gcgacagctt
    19081 gttctggaac tagtacgaat tgttcgaatc cagcttgttt caacgcacgt tcgcccaaca
    19141 tttttcctgt tccgtgaagt ggtgtgtaca caagtttcaa atctttcccc attgtttcca
    19201 ccaattcatg atcaatcgtt actgctttga tttcttctag ataagcggca tccacttctt
    19261 ctccgatgat cgtgatcaat ccgctatgtt cagcttcttc gtcagataat acttcaactt
    19321 ttaacggatt gctgacttca cggacatatt tcgtcaatgc atctgcgtct gcaggaggca
    19381 tttgtccccc atcttctccg tagactttat atccgttgta agctgctgga ttgtgggatg
    19441 cggttaccat gattcccgca aacgtcttca aatgacggac tgcaaaagaa agctctggtg
    19501 ttggacgtag actttcgaat acataagatg ggatattgtg atgtgctaat gttttggctg
    19561 cttccatggc aaattctggt gattgatggc gtgagtcata agcgatagcg acacctcggc
    19621 gttttgtttc ttcgccttgt gtatccatga aacgtgcaag tccttctgtt gcttgtctta
    19681 ccgtatagat gttcattcga ttgatcccag gccctaagat tccccgcatg cctgcagtac
    19741 cgaattccaa cggtgcataa aaagcatctt caagtttttc aggatcttca cccagatcat
    19801 ttaattgttt ttttaagttt tcttctaaat tttcttcatg tacccattgt tcatacactt
    19861 gttcccaaga catgaaaata acacctcttt ctgaaatatg gttcttaata agtatagcat
    19921 tcacttatta gatatgaaag tatgaggacc aaaaaacgct taaatcaaga aaggattctt
    19981 ttatcaagca cggttgcaag tattcgcata aatcggattc agtagatttc acgcgaaaaa
    20041 aaagagtaaa agaacaatta aaccgttctt ccactctttt taaacttatg cagttgtttc
    20101 ttctaaagat tccaagtatt tatacacttg ttgtccagcg atcccaccat caccaacagc
    20161 agtagtgatt tgtcgcaaat ctttttcacg aacatctccg atggcaaaaa cgcctggtat
    20221 attcgtcttc atttcgttgt ctgttggaat ccagccagct tcgttcgtca accctgcttc
    20281 tctaaatggt gcagttaatg gatccagtcc aacatagatg aatactccgt ctgctgccaa
    20341 atcaaactca ttgccggttt tgacattacg tgctttcaca cctgtcacta ccatttcatt
    20401 cccaacgatt tcgtcaacga ctgtatccca cataaaggaa attttttcat tcgcaaatgc
    20461 acggtcttgg atgatctttt gtgcacgtaa ttcatctcta cggtggacga tgactacttc
    20521 agaagcaaat tgtgtcaaat agatggcttc ttctactgct gagtctcctc ctccgatcac
    20581 tactagacgt ttgttacgga aaaacgcacc atcacaaact gcacagtaag aaacgccacg
    20641 tccagcaaat tcttcctcgc caggtacgcc taatttacgg tggatacagc ctgttgcgat
    20701 aatgactgtt tttccttcgt aagttttatc ttcacaaata acttctttgt ggctgccgtg
    20761 atccttgata tcttgcacga ttccataagc attttctgtc ccaaatcttg aaacaccttc
    20821 atacatctta taagctagat caggacccat tattgaatcg aaaccaggat agttttcgat
    20881 ttctgccgta ttgttcattt ggccgccagg ggcgccttgt tcgatcatca atacggataa
    20941 gtttgaacgt gaagcatata aagcagcagt catcccagca ggacctgcgc caataacgat
    21001 tacatcatac atatgtagat tctcctttac ttaatcaagt aagcatactt tacagccaat
    21061 gcgaaaaaaa gtctattgtt ctgcttgcgt ttctcgaata gctttattat actcgaaaaa
    21121 tccgctgaaa aacaatagac ttttataaat cattgatcaa tcattcgaaa gattttctcc
    21181 atttgaatca attacttttt tataccagtt gaatgatttc ttcttcgaac gggctaatgt
    21241 gccatttcct tcgttgtcac gatcgacata aatgaatccg taccttttct tcatctcacc
    21301 tgtaccagct gatactaaat caatacatcc ccaagtcgta tagcctaata gatcgactcc
    21361 atctaattct actgcatctt tcattgcctg gatatgtgct gccagatagt cgattcgata
    21421 gtcatcttcc acataaccat tctcatcggg cgtatcgact gcacctaagc cgttttctac
    21481 aataaatagt ggtttttgat aacgatcata aatatcattc atcgttattc tcaagcctaa
    21541 tggatcaatt tgccaacccc attcacttgc ttccagatat ggatttttga cagaagcaaa
    21601 gatatttcct gcagtctgtt ccaagagttc gttgtcagta gtcgaaactc gagaagaata
    21661 ataagaaaag gagataaaat caacagtatg ttctttcagc aatgcggcat ccccaggttc
    21721 cattttgatc tgaatgccat ttcgttccat ttctttcaat gcataagcag gatattctcc
    21781 tcgtgactgg acatcaatga agaaataatt ttctcgatct gattttctag aagcccaaac
    21841 atcttcagga ttgcaagtgt atggataata tgctcctgct gccagcatac agcctacttt
    21901 gttctccgga tctacttcat gagcaatctt cgttgcgatg gcacttgcaa ccaactcatg
    21961 atgtgccgct tgatatttga tttgttcttc attttctcct tcttcaaaat acagccctgc
    22021 gcccatgaat ggtgcatgaa ggatcatatt gatctcattg aatgtcaacc aatatttgac
    22081 aagtccttta tagcgattga agatgactcg gcacagattt tcataaaaac caacgagttc
    22141 tctggaacgc cacgcaccat attctttgat caaatgcatc ggacaatcaa aatgtgtaat
    22201 cgtcacaagc ggctcgatcc cgtacttttg acattcttta aagacatcct cataaaattt
    22261 caaaccagat tcatttggct ctttttcatc gcccattgga aaaatacgac tccaagcaat
    22321 ggacatccga taagtcttga accccatttc tgcaaataaa gcaatatctt ccttataatg
    22381 atgatacata tcgatcgcaa tttttgctgg ataaaaatga ttttcatcaa attcaaacat
    22441 tttctttttt ccagtaatga caggaaaacg gtcttctccg attggcacaa catctacgtt
    22501 cgctaaccca cgtccgcctt catcataacc gccctcgcat tgattggctg ctgtagctcc
    22561 gccccataaa aaatcttttc tgaatcccat catacttcct ccattacatg tgtattgctt
    22621 gataattctc ttgccagttc atcttggact tcgattcctt ctgcttttaa ccggctgatt
    22681 gtttccttga attgcggtaa atactgctga tgtgcaataa acaactcatt catgatccgt
    22741 tttgctgtct ttcctgatcg tataagcgga ttgatcgtaa acgcttgtaa agctgttcca
    22801 taatcaccag tcacagctgc ttcgatcgtc agcaattcca tattcttcat tacttgcaac
    22861 cagcctcttt cagcagcagg aagggaacca aacgctacat ttcgtgcgcc ttgtgcgcca
    22921 acataggcag tgacttctac tacacaatct gccggcagat caggaacagc tccatcattc
    22981 tttgtagata ccacgatctg tgtttcttta tttccataga tcgaagcaat cgtttcacag
    23041 gcagcatctg aataataagc accacctcgt ttttgcaatt gttcaggttt atgatccaat
    23101 tctggatctt tgtataattc aaacagctct gcttctgttt ctttcacttg ctgcgctctt
    23161 gtaccaatcg tttgatattc ttctaaagca tgttccagca tttcttcttc acggtagtaa
    23221 tagcggtggt agccacaagg gatcatgttc atttgtttta attgttcttt gaaaaacggg
    23281 atatcaaaga tatttttcgg caatccattg tcttcatcgt ataaacgatc aatcaattct
    23341 gccgtgcggt catttccttt attatctgct actttgtgcc aatggaagtg atttagaccg
    23401 gcaaacttgt agatcagctc ttcttttttc ttgccaagca ttgttggttc tgtcatcatc
    23461 gccattactg gcacattgca taatcctatc actttatccc atttgccata tctgataact
    23521 gcttctgtca ccatcccgct tgggttcgcg aaattgatca gccaagcatt tggacataaa
    23581 cgcttcatgt cttcaacgat tgccaaaacc actggaacgg tacgaaaagc tttgaatatc
    23641 ccgccagcac catttgtttc ttgcccaatc atcccataat aactagggat ccgctcgtct
    23701 ttgattcgtg catctaattg accgacacga aactgagtcg taacaaaatc cgcatctttc
    23761 aatgcttctt cccgatccag agttgcatag attttgactt catacggaga agcatcccac
    23821 atacgctgag acatctgact gacgatctca agcttttctt ttccttcttc tacatctacg
    23881 agccagattt ctcgtatcgg taattcctca tatcttttga taaatccttc catcaactcg
    23941 ggggtgtaac tgctgcctcc accaatcgta gcgattttga ttccttttgc catgttctca
    24001 ctcctttact ttttacactt ttattatctt ttttgtaaac aaaacaaaca atagaaattc
    24061 gtttctttcc agtaacattt acaaaagtta gtgttttcta ctggattttt gtaaatatct
    24121 tgtcaaaaaa tgtctttatt aagctatata acataaatcc gtttacaaat cattttcaaa
    24181 aaagttataa tttacgtaaa aaaacaagaa gaaggtatct acatgttatt acgtgaaaaa
    24241 atcaaagaaa tctccttttc tccttcagaa aatgaagtga ttcagtttct cttaagcgat
    24301 tctccagcac ttggcgaaat gaccatccaa gagattgctg agcgatgtta tgtccacccg
    24361 tccacactca tccgtgtagc aaaaaaaatg ggttttgatg gctgggtcag ttttcgtaat
    24421 gcttttttag ctgaaacagc ctatcttgaa gggaattttc aagacgtaga tgcgaatctt
    24481 cctttttcca ccacagacgg tattatgacg atcgcaaata aaatggcttc tttagaacag
    24541 atgacgattc aagatacact ttctctcatt catcatgacc aactgcaaaa agccaaacaa
    24601 ctaatgcttc aagcaaaaaa aatcgtgatt ttcgccagta actcgaatac tttgatttct
    24661 caagatttca tgctgaaaat gaagcgcatc aaaatggatg tcacgattgt caccactttt
    24721 ggtgaatcgt tctatgaagc gtacaattgc acgagtgata tgtgcgggat tctgatttct
    24781 tatacgggtg aaaacaatgt gatccttcgt accgcagaaa tattgaaaca aaatcatgtt
    24841 ccaattttag ctttgaccag tatcggtgat tcaacgctat cttcaatgag tacagccgct
    24901 ttgcgactta cgacacgcga acgactgtat tcgaaaattg gtaattttac gatcaataca
    24961 tcgatttgtt atcttctaga cgtgttatat agttgtgttt ttgcagaaaa ttatcaacag
    25021 aatttagtcc atctaatttc tgtaggacaa aaatttgata aacgccctat cagctcagcc
    25081 atcatgaaag agcctttcaa ttttgaaaat aaagaagtgg actaaaaaga ataattaagc
    25141 ctagtccatt ctttcaccta accgttcagg tcagtgcggt gataagtgca tcgtctggcc
    25201 cgaccgtctc cccttgattt tccaaaatat ccaaataatc cgtgtaattg gtgacgatca
    25261 caggtgtttc taaactgtat ccttcctctt cgattttctt tttgtcgaag cggagcaaca
    25321 gatcgccttg tttcactctt tctccttgct gcaccaagct ttcaaaatat ttcccttcca
    25381 actgaacagt atccaatccg acatgaatca gcaattccag acctgcttca gaaaccattc
    25441 cgattgcatg tttcgtcggg aacagggtca tgactgttcc gtcaaaaggg gcacgtactt
    25501 ctcctttttc agggtagatc aagattcctc gacctagtgt tccttgtgca aaagctgcat
    25561 cctttgctgc attcagtgga gcgatttgtc cttgtatcgg tgtttttaca atttctttac
    25621 ctgatctttt ctgtctatca tctgtttcac ctgcttcctc cttccaaaag aaaaaagtca
    25681 gcgcaaagga taccaccatc gcaatggcga ttccaatcaa tacattaatc acatccgtta
    25741 aatcaccatc tggtctaata tagttgggaa ttgtaaagat gcctaatcca cctggattcc
    25801 aagaagttaa attcattaat ccactatacg cgccccctaa cgcaccaccg acacaggtaa
    25861 aaataaatat ttttttacgt ggcaatgtaa acccataaat agctggctct gtaatcccca
    25921 caataccaga aacaatggct ggtattgcga ttgctttgtc ttttgggttt ctaagcttaa
    25981 agtagagtgc agtaagtgct gcagtttgtc caaaagacac tactgcaaca ggtgtcatga
    26041 tattttgcca accattcaca gaaaattgca tcaatcccat tggtacgatt gcccaatgga
    26101 gaccaaacat cactagaatt tgccagaaga agcctacgat tgctccataa ataactggcg
    26161 aaagtgctaa aagtgtttca aatccagcca ttaaaatatc agttgcgaat gtgaatgctg
    26221 gtccaacgag taaaaaacct aagggcattg taataagaac ggtgaagaat ggaacgaaga
    26281 aattcgcgat catctcaggt actaactttt taattggttt ctgaatttgg ctagctaaca
    26341 acacaataaa gataatggga atgacagaag aagcgtagtt acgcgcaatc cagggaatgc
    26401 cgaatactgt ttgataaata ggtgcttcaa aaatagtgcc agaaaatatg gtagtcagtg
    26461 gtgcttctgc tgcaccactc aaagcttcca gcgacaagct aggattcatt aatgctgccc
    26521 caagcaacat ccctacgaat tcatttactt taaatttacg ggcagctgtt aaaccgataa
    26581 taacaggcat aaattggaat acagaatcac cgatattatc aaacataaca aatgtgccag
    26641 aaccgcggtc aaatgcgcct aagaagacaa gtaatgctgt cagtccacga atcatcccac
    26701 cagcagctag aatagcaagg aatggctgga aacagcccga tattccatca ataagcatat
    26761 caaacggtct cattttttct gttgaaacag cctcatcaaa agtaaggttt cctgctttta
    26821 taacctcttc aaacacgact ggcacatgat tcccgatgac aacttggtac tgtcctcccg
    26881 ctcgcattac cgtcacaaca ccgtccatat ttttcaagat ctcatcattt gctttttctt
    26941 cgttttttaa tttgaaacga agccttgtga tacaatgtgt caaaccattg atattttcct
    27001 tcccgccgac attttctaca attttgctcg ctaattcttg atattttccc atttctattc
    27061 cctccaattt attagtggaa ggtttagcca actgaatgtc actaccctta tttcatgcgt
    27121 tcttgttaat ttatcctcct ttttttattt atttacaacg attctcatgc actaaacgcg
    27181 caatatggat cgttaaatac attttttcgt ccgtggacat ctggtagttg aatatctcgg
    27241 ctaaaaagtc tgctattttc tcaacgcaac caaacgtatc cttatatttt ttttgaataa
    27301 cctggtacaa ttcctcagaa acttctccca cttctgattt ttctccatct aggacgcgtg
    27361 tcagaaaaaa acgtaggtgt gtgacgaatc gaaaaaaatg gactgaatct tcatcaaatg
    27421 taatcttata gtaatagcgg acgatattta gaatatcctg tattgttgta gtaaacaggt
    27481 aggtattgtc ttgttggctt tgaatgcctt gtgcattgac taaatgcaat gcgataaatc
    27541 cggcttcatc ttctgataac ttgatgtggt atttatccga gattttcttc actgtttttt
    27601 tcccgatggc atactcttta ggaaatagtt gtttgatatc atacagcaat acatttggta
    27661 caaatattcc cattttgtga cgttcgatag ccgtatacaa atgatccgtc aatgcaatat
    27721 acaccgaatc attcaactgg acttgtagag cctcttttgt atattcaatg atttcacaag
    27781 aaatttctaa gtactcaaga ggaatcgctt gtgccaattc ttggaactgt tgtgaaagtc
    27841 cgtcatttgt caactgaaat tttttgtcga tgcatgagtc aaaaatcggg tcacccactt
    27901 tctttttgaa tgccaaacct tttcccatca cgatcatttc ctgatttctt tcatccctgg
    27961 ttattacgac gttgttattc agtactttct caatcagcat gttttactcc tttccatgat
    28021 atatagggct gtatccaaaa caaaaagaaa aacccaaaaa gccgtccatg gaaccatggg
    28081 agactcatca ggtttagctt gcatcaggca atcactatcc aagttgtatc gttttcttta
    28141 tattatcgaa agcaagcact tttgtcaacg ctttcttagc ggctattggg cacttctgac
    28201 tagctgtcgt gatttttttt aaatctggta attacgacac taacgctcac agatgaagaa
    28261 tctgggaagc tctgttttat tttcactcta gcgcttaggt caaaatgcta ctatgctact
    28321 gtcccaactt ctaatatacg gtgttccagt accagcttat cggtgataag ccaaatttat
    28381 gcaaaaatct ggaaagcctt ttttacaatt tttttcttat cacctaaagc tgaacggagc
    28441 cctgtcacaa ccacttttta cacaccactg ctttgcagtt tgtacatatc tgcatacaaa
    28501 ccattttgcg ctagaagtgt ttcatgattc ccacgttcta ctatatgacc tttgtctaaa
    28561 actaaaatca attctgcatc ccgaatcgtg gacagccgat gggcaatcgc aatcgttgtc
    28621 cgtccttgtc gcatttttgt caatccttct tggatcaatc cttctgtttc agtatcgata
    28681 tttgctgtcg cttcatccaa tactagtatt ttaggatctg tcacgattgt tcgcgcaaaa
    28741 gtgatcagct gtcgttgtcc gctagaatag ctggcaccgt gttcgatcac ttttgagtga
    28801 tatccttttg gtaattcatc gatgaacttg tttgcttgaa caaactcagc tgcatggcga
    28861 atctgttcgt ctgtgatagt tggattcatc atccggatat ttcctgcgat atctccataa
    28921 aacaagaagg catcttgcaa gaccaatccc attttcttac gtaactcttc cattggatat
    28981 tctcgaatgt catgatcatc aatcaagatc tggccttcat aaaattcgta aaaccgcatc
    29041 aatacgttga tgatcgagct ttttccgctt ccggtgtgcc ccactaatgc aacggtctct
    29101 cccggattag cgacaaaaga gatattgttc aatacattgt gctctccatc ataagaaaag
    29161 cttacattgc gaaactcgat cttacctgaa gtaatcgctt gatctgctcc aggattttgt
    29221 tgcggtgcat attcttctgt atccatgatt ttcaaaatac gactaccggc aaccattcca
    29281 tctgtaaagg tgctcaaaaa gtccatcatt tgagacattg gattaaaaaa tccctggaca
    29341 taggtcgtaa atgcatagat cagtccaact tctacgggag aatgcagcgc atcgatccca
    29401 aacaaagtca acgccaacgc aatcgccaat gcatatagaa aattgatgat cggacttaac
    29461 agaatcgaat ttgttttgat catcgcatag cgcgtgtcta aatactcgtt gttcacttgt
    29521 tcaaattctt tagctaaacg agcttcttgg cgaaactgct ggataatacg catgccggaa
    29581 atcgattcat tcaatttcgt gttcagctga cttaattttt cccgcatatt ccggtaaata
    29641 cgagaactga atttctggta ataccagatg acgattccta aaatcggcaa gaacaccaaa
    29701 gtccacaagg caatttctcc attgatttga aacatggcaa tgaaggaaga aacgactgca
    29761 aagatcccag tcaataccat caaaaagaca taccaaaatt caaatagcgt ttctgtgtca
    29821 tttgtcactc gagaaacagt cgatcctgcc ggtgtctgat caaaataacg catacctagt
    29881 gtatgcagct tttcaaataa tcttacccga acatactgat aggtcttcaa tgaagccatg
    29941 gaatacaaat accattggaa aaaccaaatg atacttttga ctatgactcc aaacaagtac
    30001 ataccagcaa aaaacaaaat aacttgtgtc gtagccgact gtttagccag atgatcatcc
    30061 ataaaagttt ggatgatcct tggcaatagg acattgatca cagacagggc aaacgcaaat
    30121 aagatagcaa cgatgaacgt tcttctaaaa ggtttggcga acttcatcag tcgtttgatg
    30181 atcttcttct gttctttaag tggaatcgat tttgtccatt ccgattgata attttcatcc
    30241 atcaggcctc gcctccttct atcttcgctt ctaatttctg tttctcccac attcgttcat
    30301 accagccgcc taatgcaagc agctcatgat gggttcctcg ttcaatgatt ttcccttgat
    30361 cgacaacaat gatttctttt gcatgcatca cgctgcttaa acggtgagct gcaatgatgg
    30421 ttgttttctc ttttcttatt tcttttaaat tatgcaagat agcttcttct gttttggcat
    30481 ctactgcaga aagtgcatca tctaagatca gcaattcagg atttttgatc aatgctcgag
    30541 caatcgaaat cctttgtttc tgtccgccag ataaagagac tccacgttct ccaactaagg
    30601 tatcataacc ttttggtaat tccttgattt cattgttgat aaatgctaaa gaagccgcat
    30661 gctctacttc ttcttctgaa agttttggat tagaaaaacg gatattatct cgaatggtca
    30721 tcgaaaacaa gaagtgatct tgaggcacat agccgattga tcctaataaa gcattcaatg
    30781 aatattcttt gatcgaacat ccgccaaatt gaatttttcc ttgataatca tcgtattcac
    30841 gcatcagtaa tttgatgatt gtcgtttttc ccgcaccagt tttcccaaca atacctaatg
    30901 tttctccttc ttggatcgca aatttgattt gttctaatgc tggtgattct tctttcggat
    30961 agttaaaaga ggaaatagct acattcagct cgccttttgc cggagtctgc actgcaccca
    31021 gttgttccac gatatgcgtc ttttcatgca aaagttcatt gacgcgatca taagaagcat
    31081 ttcctctttc caatacgttg aacaatcgac caatcgcaaa catcggccaa acaagcatac
    31141 cgatatagct aataaatgag accatctgcc cgattgagat cgtgccttcc aagacatact
    31201 ttccacctaa aataattgtt gcaacataag acagaccgat aatcaaggta atgaatggat
    31261 caaataagga gtctaaaaaa ttgactcgtt tgtttttaat aattgcatct tcaatctttt
    31321 cttcaaaatc cgcgagatct tctttttctt gtccaaatgt tttaatgact ttgatcccag
    31381 taatactttc ttgtgtctta tcattgatac tagaaaaagc ttcttgcgaa tcacgaaaag
    31441 catcgtggag ttttgacccc agcactcgtg aagtaacggc tagtaaaggc agaggaatca
    31501 gcgcaatcag tgttaagcgc caatcaataa acaaaatcat cgcaatgatc gttgttccgc
    31561 ccgtgatcac tgaatctgca aaggtcaaga taccagctcc tgctacattt tggattgcat
    31621 tcaaatcatt cgttgcatga gccatcaaat ctccagtccg atatttttgg taaaagagat
    31681 tgtccatttc tgtaaaatgt ttgaatagct gctgccgaag ttctttctcc aaccgagctg
    31741 cacttcccca aatattcgtc cgccagatat aacgaaaaag atattgtaag agagctgcaa
    31801 ggatcaataa taagatccac caggaaatga tctttattga taaattttta tcggcgatct
    31861 catcaatgat gatcccaatc acttttggag gagctaactg taccaaggcc actgcgaata
    31921 gggagaaaat cccaattaaa taatgtttct tttcctgtcg aaaaaaccaa cctaattttc
    31981 taaaaatcga cattttactc ctcctttgtc tttatcaaga gcataggatt gtctgtgctt
    32041 gtcttttttg ctgaatagtc tgaatagtct aacaaattac gaaataaaaa acggactgaa
    32101 aaaatcagcc cgcaaaaagt ctttgggtca gaacctattt tttcccttgg ttcttcattt
    32161 gctgcatcat ctggcgaatt ttcttctcag atggtttttg ccccattgac atcatcattg
    32221 atcgcaacat gtcctcatta acgggaggat ttttttctaa atagtctttc atatatttac
    32281 gcgctaggaa aaagccacct actgcgccga ttagcaaagc aataatagca ataagtacga
    32341 caatacctgt tgataccata taaatctctc ctttctattt ccgaattgct ctcaatgatt
    32401 tattttacta aagtttattg aaaaaagaaa ggcgtattct ttagtttcat gcaaaaaata
    32461 gtggtttttt tagagaagaa caaaatttct ttcattaacc agcaagacac gccacgaaaa
    32521 aaaattaggc ataccaaatc tttttcttta attttttctt tctgaggtgt agaatgtaat
    32581 cgtagataaa taggaaaggt ggttttttct tgaataatca atccaacaaa caatttcata
    32641 agcatcgact tattgaacat acaaatggtt tatctttagc tgaagtaaat ggaacagtgg
    32701 atgtacccaa gggaaaaagt ttttttaaaa cacttcttgc ttactctgga ccaggtgcat
    32761 tagtagcagt tggttatatg gatccaggta actggtccac ttcgatcact ggtggacaaa
    32821 atttccaata tttattgatg tcagttattt tgatgtctag tctgattgct atgcttttac
    32881 aatatatggc agcaaaatta ggaatcgtga cccaaatgga tttggcccaa gcaatccgag
    32941 cacggacaag taagacattg ggtgtcattt tatggatctt gactgaatta gccattatgg
    33001 caacagacat cgcagaagtc attggtgcag caatcgccct ctacctgctt tttgatattc
    33061 cattggtaat tgccgtattt atcactgtgt tagatgtttt cgtcctttta ttgctgacaa
    33121 aagtcgggtt tcgtaaaatc gaagctttgg ttgtctgctt gattttcgtc attttattcg
    33181 ttttcgtcta tcaagttggc ttgtctaacc ctgattggca cagtttgttt gccggattta
    33241 tccctaacag tcacacattc tcagactcac cggtcatcgg cggcgaaact ccgctgactg
    33301 gagctttagg gattattggt gcaacagtca tgccgcataa cttatatctt cattcagcag
    33361 tttctcaaac tcggaagatc gatcaccaag acgaagaaga tgtagcacaa gcggtacgct
    33421 tttctacttg ggattctaat atccagttgt caatggcttt tctagtcaat gcactgctat
    33481 tgatcatggg tgtggctgta ttcaaaaccg gttctgtcca agatccttcc ttttttggtc
    33541 tgtatgaagc tttatctgat ccatcagcta tgagcaatgg tatcttgatt gctgtagcaa
    33601 aatctggtat cttgtctact ttatttgctg tagcacttct tgcttctggc cagaactcta
    33661 ctattactgg tacgttgacc ggtcaagtca tcatggaagg cttcatccat atgcgtatcc
    33721 caatttggct aagaagacta gtcacacgat tgctttctgt gattcctgta ttgatttgtg
    33781 ttttgtttac aagtacaaaa ggaacgatcg aagaacatac cgctttgaat aacttgatga
    33841 atgaatccca agtttttctg gcttttgcat tgccattctc gatgattcct ttactgatga
    33901 tgaccaatag ccaagcggag atggggagcc gattcaaaaa ttcatttgtt gtaaaactac
    33961 ttggctggtt ctctgtgatt agtctgactt acttgaattt acgaggatta ccaggtcaaa
    34021 tcgaaagttt cttcggcgag caagccacct cagctcaact agcattggct gatcaaatcg
    34081 cttatgtaat cattgttctt gtccttctcc tgttggcatg gacgattggt gatttatata
    34141 aaggaaacaa aaaatatgaa cagcgtttgc aggaaacgat agaagaaatc gagtagaaac
    34201 tgattctttt acacaaagga ggttgtgtca gaagtgggca gcttcaagaa ataagaagga
    34261 atttaagaaa attgcttttc aaatttttgt gaaatttcgg cttatttccg aagaagttac
    34321 ttctggaaca ccgtgtatca gaggttgtga aaaagctgcc ttgcatcaag taataagaac
    34381 agccaagtaa aaacagcttt ttatgttttt aacttggttg gacttattac cgcagatgca
    34441 agcttttgag caccgtttat tcggaaaaag gaactgcgac aagacttttg tcacagttcc
    34501 ctttcctacg attagtcaag taaaaggtgt aagaaaaata gctcataaac gaaagcgaca
    34561 aaatcaa
//
LOCUS       DAZUJO010000022        34510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000022 DAZUJO010000000
VERSION     DAZUJO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.022"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8OL_001156"
     CDS             212..604
                     /locus_tag="ACG8OL_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4817157.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8OL_001157"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8OL_001157"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS4817158.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8OL_001158"
     CDS             2698..2943
                     /locus_tag="ACG8OL_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817159.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8OL_001159"
     CDS             3165..4775
                     /locus_tag="ACG8OL_001159"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS4817160.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8OL_001160"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8OL_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817161.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8OL_001161"
     CDS             5067..5936
                     /locus_tag="ACG8OL_001161"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS4817162.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8OL_001162"
     CDS             6101..7372
                     /locus_tag="ACG8OL_001162"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4817163.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8OL_001163"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8OL_001163"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS4817164.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8OL_001164"
     CDS             8763..9026
                     /locus_tag="ACG8OL_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS4817165.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8OL_001165"
     CDS             9471..9920
                     /locus_tag="ACG8OL_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4817166.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8OL_001166"
     CDS             9977..11395
                     /locus_tag="ACG8OL_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4817167.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8OL_001167"
     CDS             11395..12255
                     /locus_tag="ACG8OL_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS4817168.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8OL_001168"
     CDS             12429..13202
                     /locus_tag="ACG8OL_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4817169.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8OL_001169"
     CDS             13349..13714
                     /locus_tag="ACG8OL_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS4817170.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8OL_001170"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8OL_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4817171.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8OL_001171"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8OL_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817172.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8OL_001172"
     CDS             15560..16216
                     /locus_tag="ACG8OL_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4817173.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8OL_001173"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8OL_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS4817174.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8OL_001174"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8OL_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS4817175.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8OL_001175"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8OL_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS4817176.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8OL_001176"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8OL_001176"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS4817177.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8OL_001177"
     CDS             21888..22202
                     /locus_tag="ACG8OL_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS4817178.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8OL_001178"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8OL_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS4817179.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8OL_001179"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8OL_001179"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS4817180.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8OL_001180"
     CDS             23546..23875
                     /locus_tag="ACG8OL_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS4817181.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8OL_001181"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8OL_001181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS4817182.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8OL_001182"
     CDS             24863..25687
                     /locus_tag="ACG8OL_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS4817183.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8OL_001183"
     CDS             25680..26027
                     /locus_tag="ACG8OL_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS4817184.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8OL_001184"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8OL_001184"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS4817185.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8OL_001185"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8OL_001185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS4817186.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8OL_001186"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8OL_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS4817187.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8OL_001187"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8OL_001187"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS4817188.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8OL_001188"
     CDS             31684..32451
                     /locus_tag="ACG8OL_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817189.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8OL_001189"
     CDS             32441..34483
                     /locus_tag="ACG8OL_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817190.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11095 a         6120 c         7248 g        10047 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat
//
LOCUS       DAZUJO010000023        33987 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000023 DAZUJO010000000
VERSION     DAZUJO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.023"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACG8OL_001190"
     CDS             complement(27..1259)
                     /locus_tag="ACG8OL_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4817191.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACG8OL_001191"
     CDS             1514..2083
                     /locus_tag="ACG8OL_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4817192.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACG8OL_001192"
     CDS             complement(2261..2701)
                     /locus_tag="ACG8OL_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4817193.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACG8OL_001193"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACG8OL_001193"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4817194.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACG8OL_001194"
     CDS             3655..4578
                     /locus_tag="ACG8OL_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4817195.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACG8OL_001195"
     CDS             complement(4803..5042)
                     /locus_tag="ACG8OL_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817196.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACG8OL_001196"
     CDS             complement(5047..5466)
                     /locus_tag="ACG8OL_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4817197.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACG8OL_001197"
                     /gene_synonym="mefH"
     CDS             complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACG8OL_001197"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS4817198.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="ACG8OL_001198"
     CDS             complement(7038..7673)
                     /locus_tag="ACG8OL_001198"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4817199.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7787..7879)
                     /locus_tag="ACG8OL_001199"
                     /pseudo
     CDS             complement(7787..7879)
                     /locus_tag="ACG8OL_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8015..8650)
                     /locus_tag="ACG8OL_001200"
     CDS             complement(8015..8650)
                     /locus_tag="ACG8OL_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4817200.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="ACG8OL_001201"
     CDS             9162..10304
                     /locus_tag="ACG8OL_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4817201.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="ACG8OL_001202"
     CDS             10297..10503
                     /locus_tag="ACG8OL_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4817202.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="ACG8OL_001203"
     CDS             10582..11775
                     /locus_tag="ACG8OL_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4817203.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11980..13119
                     /locus_tag="ACG8OL_001204"
     CDS             11980..13119
                     /locus_tag="ACG8OL_001204"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4817204.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="ACG8OL_001205"
     CDS             13112..13912
                     /locus_tag="ACG8OL_001205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4817205.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="ACG8OL_001206"
     CDS             13912..14739
                     /locus_tag="ACG8OL_001206"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4817206.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14744..15451
                     /locus_tag="ACG8OL_001207"
     CDS             14744..15451
                     /locus_tag="ACG8OL_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4817207.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="ACG8OL_001208"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="ACG8OL_001208"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4817208.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="ACG8OL_001209"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="ACG8OL_001209"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4817209.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="ACG8OL_001210"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="ACG8OL_001210"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4817210.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="ACG8OL_001211"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="ACG8OL_001211"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4817211.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="ACG8OL_001212"
     CDS             19036..21069
                     /locus_tag="ACG8OL_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817212.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="ACG8OL_001213"
     CDS             21113..22393
                     /locus_tag="ACG8OL_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4817213.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="ACG8OL_001214"
     CDS             22603..23409
                     /locus_tag="ACG8OL_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817214.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="ACG8OL_001215"
     CDS             23421..24641
                     /locus_tag="ACG8OL_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817215.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="ACG8OL_001216"
     CDS             24631..26217
                     /locus_tag="ACG8OL_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4817216.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="ACG8OL_001217"
     CDS             26256..28394
                     /locus_tag="ACG8OL_001217"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4817217.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28762..29745)
                     /locus_tag="ACG8OL_001218"
     CDS             complement(28762..29745)
                     /locus_tag="ACG8OL_001218"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4817218.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30123..31514
                     /locus_tag="ACG8OL_001219"
     CDS             30123..31514
                     /locus_tag="ACG8OL_001219"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS4817219.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31530..32570
                     /locus_tag="ACG8OL_001220"
     CDS             31530..32570
                     /locus_tag="ACG8OL_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4817220.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32589..33674
                     /locus_tag="ACG8OL_001221"
     CDS             32589..33674
                     /locus_tag="ACG8OL_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS4817221.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33707..>33987
                     /locus_tag="ACG8OL_001222"
     CDS             33707..>33987
                     /locus_tag="ACG8OL_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFS4817222.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLS"
BASE COUNT        11016 a         5824 c         6698 g        10449 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca
    28561 aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg
    28621 tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca
    28681 gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc
    28741 gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa
    28801 agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt
    28861 cttagcgtca atataaagaa catttccact attaccaatt gctgaatata tttcgttctc
    28921 agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact
    28981 ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata
    29041 catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa
    29101 accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga
    29161 cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt
    29221 tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact
    29281 tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc
    29341 ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga
    29401 acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg
    29461 cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata
    29521 atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag
    29581 caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc
    29641 attccattca tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt
    29701 tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac
    29761 tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta
    29821 atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata
    29881 aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc
    29941 aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag
    30001 aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc ttataaacta
    30061 ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat
    30121 atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg
    30181 ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc
    30241 cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg
    30301 cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt
    30361 ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg
    30421 gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt
    30481 tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa
    30541 aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa
    30601 agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca
    30661 aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa
    30721 aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat
    30781 ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg
    30841 aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca
    30901 tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca gcaattttag
    30961 tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa
    31021 aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac
    31081 cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg
    31141 tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag
    31201 gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact
    31261 acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg
    31321 tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga
    31381 tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc
    31441 aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa
    31501 aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac
    31561 cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt
    31621 atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca
    31681 ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc
    31741 ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg
    31801 atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga
    31861 gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa
    31921 tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta
    31981 taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct
    32041 atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg
    32101 atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag
    32161 tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca
    32221 agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata
    32281 aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata
    32341 gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc
    32401 cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa
    32461 agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg
    32521 ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg
    32581 aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt
    32641 ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga
    32701 tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc
    32761 gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata
    32821 agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa
    32881 ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa
    32941 ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat
    33001 tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt
    33061 ttcaaaacca cccctttgag atagttcata atggtattga tttagagctt tatagtaaaa
    33121 attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg
    33181 tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt
    33241 tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta
    33301 gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag
    33361 gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc
    33421 tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct
    33481 tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag
    33541 atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa
    33601 cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag agtatatata
    33661 gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga aaatatgtta
    33721 tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc gattgaacga
    33781 aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat ttattgaagc
    33841 atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta aaatttattg
    33901 gggtggtttt tccattgtac aagcagaaat caatttagta aagagagcat tacaaaatga
    33961 gaaatattta aaatatgtat tattatc
//
LOCUS       DAZUJO010000024        32503 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000024 DAZUJO010000000
VERSION     DAZUJO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.024"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACG8OL_001223"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACG8OL_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS4817223.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACG8OL_001224"
     CDS             500..862
                     /locus_tag="ACG8OL_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4817224.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACG8OL_001225"
     CDS             914..2596
                     /locus_tag="ACG8OL_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS4817225.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACG8OL_001226"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACG8OL_001226"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS4817226.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACG8OL_001227"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACG8OL_001227"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS4817227.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACG8OL_001228"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACG8OL_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS4817228.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="ACG8OL_001229"
     CDS             6431..7435
                     /locus_tag="ACG8OL_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817229.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="ACG8OL_001230"
     CDS             7436..8380
                     /locus_tag="ACG8OL_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817230.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /gene="opp4B"
                     /locus_tag="ACG8OL_001231"
     CDS             8380..9342
                     /gene="opp4B"
                     /locus_tag="ACG8OL_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4817231.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="ACG8OL_001232"
     CDS             9369..10283
                     /locus_tag="ACG8OL_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817232.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="ACG8OL_001233"
     CDS             10309..12090
                     /locus_tag="ACG8OL_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4817233.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /gene="rnc"
                     /locus_tag="ACG8OL_001234"
     CDS             12438..13124
                     /gene="rnc"
                     /locus_tag="ACG8OL_001234"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS4817234.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="ACG8OL_001235"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="ACG8OL_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS4817235.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="ACG8OL_001236"
     CDS             16734..17543
                     /locus_tag="ACG8OL_001236"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4817236.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="ACG8OL_001237"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="ACG8OL_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS4817237.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="ACG8OL_001238"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="ACG8OL_001238"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS4817238.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="ACG8OL_001239"
     CDS             complement(21095..21547)
                     /locus_tag="ACG8OL_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS4817239.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="ACG8OL_001240"
     CDS             21697..22647
                     /locus_tag="ACG8OL_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4817240.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="ACG8OL_001241"
     CDS             22640..23599
                     /locus_tag="ACG8OL_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4817241.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="ACG8OL_001242"
     CDS             23596..24351
                     /locus_tag="ACG8OL_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817242.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="ACG8OL_001243"
     CDS             24374..25327
                     /locus_tag="ACG8OL_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4817243.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="ACG8OL_001244"
     CDS             complement(25971..26267)
                     /locus_tag="ACG8OL_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS4817244.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="ACG8OL_001245"
     CDS             26622..26978
                     /locus_tag="ACG8OL_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4817245.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="ACG8OL_001246"
     CDS             26988..27887
                     /locus_tag="ACG8OL_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4817246.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28407..29135
                     /locus_tag="ACG8OL_001247"
     CDS             28407..29135
                     /locus_tag="ACG8OL_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4817247.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="ACG8OL_001248"
     CDS             29280..31307
                     /locus_tag="ACG8OL_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS4817248.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..>32503
                     /locus_tag="ACG8OL_001249"
     CDS             31415..>32503
                     /locus_tag="ACG8OL_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4817249.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEK"
BASE COUNT        10839 a         5666 c         6991 g         9007 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaa
//
LOCUS       DAZUJO010000025        31058 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000025 DAZUJO010000000
VERSION     DAZUJO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.025"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(278..640)
                     /locus_tag="ACG8OL_001250"
     CDS             complement(278..640)
                     /locus_tag="ACG8OL_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817250.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(714..1094)
                     /locus_tag="ACG8OL_001251"
     CDS             complement(714..1094)
                     /locus_tag="ACG8OL_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS4817251.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1176..2351
                     /locus_tag="ACG8OL_001252"
     CDS             1176..2351
                     /locus_tag="ACG8OL_001252"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS4817252.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2428..3012)
                     /locus_tag="ACG8OL_001253"
     CDS             complement(2428..3012)
                     /locus_tag="ACG8OL_001253"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4817253.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3213..4211
                     /locus_tag="ACG8OL_001254"
     CDS             3213..4211
                     /locus_tag="ACG8OL_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4817254.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4323..4832
                     /locus_tag="ACG8OL_001255"
     CDS             4323..4832
                     /locus_tag="ACG8OL_001255"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS4817255.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4862..5521)
                     /locus_tag="ACG8OL_001256"
     CDS             complement(4862..5521)
                     /locus_tag="ACG8OL_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS4817256.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5523..6287)
                     /locus_tag="ACG8OL_001257"
     CDS             complement(5523..6287)
                     /locus_tag="ACG8OL_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS4817257.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6295..6972)
                     /locus_tag="ACG8OL_001258"
     CDS             complement(6295..6972)
                     /locus_tag="ACG8OL_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS4817258.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7009..8394)
                     /locus_tag="ACG8OL_001259"
     CDS             complement(7009..8394)
                     /locus_tag="ACG8OL_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS4817259.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8604..8688)
                     /gene="ffs"
                     /locus_tag="ACG8OL_001260"
     ncRNA           complement(8604..8688)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8OL_001260"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8711..9226)
                     /gene="tadA"
                     /locus_tag="ACG8OL_001261"
     CDS             complement(8711..9226)
                     /gene="tadA"
                     /locus_tag="ACG8OL_001261"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS4817260.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9333..10190
                     /locus_tag="ACG8OL_001262"
     CDS             9333..10190
                     /locus_tag="ACG8OL_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817261.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10271..10606)
                     /locus_tag="ACG8OL_001263"
     CDS             complement(10271..10606)
                     /locus_tag="ACG8OL_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817262.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10673..12043)
                     /locus_tag="ACG8OL_001264"
     CDS             complement(10673..12043)
                     /locus_tag="ACG8OL_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS4817263.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12121..12657)
                     /locus_tag="ACG8OL_001265"
     CDS             complement(12121..12657)
                     /locus_tag="ACG8OL_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4817264.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12966..14447
                     /locus_tag="ACG8OL_001266"
     CDS             12966..14447
                     /locus_tag="ACG8OL_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817265.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14550..15257)
                     /locus_tag="ACG8OL_001267"
     CDS             complement(14550..15257)
                     /locus_tag="ACG8OL_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS4817266.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15336..15674)
                     /locus_tag="ACG8OL_001268"
     CDS             complement(15336..15674)
                     /locus_tag="ACG8OL_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS4817267.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15785..16747)
                     /locus_tag="ACG8OL_001269"
     CDS             complement(15785..16747)
                     /locus_tag="ACG8OL_001269"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS4817268.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16762..18093)
                     /locus_tag="ACG8OL_001270"
     CDS             complement(16762..18093)
                     /locus_tag="ACG8OL_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS4817269.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18101..18781)
                     /locus_tag="ACG8OL_001271"
     CDS             complement(18101..18781)
                     /locus_tag="ACG8OL_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS4817270.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19000..19479)
                     /locus_tag="ACG8OL_001272"
     CDS             complement(19000..19479)
                     /locus_tag="ACG8OL_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS4817271.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19606..21285)
                     /locus_tag="ACG8OL_001273"
     CDS             complement(19606..21285)
                     /locus_tag="ACG8OL_001273"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4817272.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21833..23335
                     /locus_tag="ACG8OL_001274"
     CDS             21833..23335
                     /locus_tag="ACG8OL_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817273.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23348..24010
                     /locus_tag="ACG8OL_001275"
     CDS             23348..24010
                     /locus_tag="ACG8OL_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817274.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24111..24314)
                     /locus_tag="ACG8OL_001276"
     CDS             complement(24111..24314)
                     /locus_tag="ACG8OL_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS4817275.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24425..25609)
                     /gene="iadA"
                     /locus_tag="ACG8OL_001277"
     CDS             complement(24425..25609)
                     /gene="iadA"
                     /locus_tag="ACG8OL_001277"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS4817276.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25766..27505)
                     /gene="yfcC"
                     /locus_tag="ACG8OL_001278"
     CDS             complement(25766..27505)
                     /gene="yfcC"
                     /locus_tag="ACG8OL_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS4817277.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28278..28892)
                     /locus_tag="ACG8OL_001279"
     CDS             complement(28278..28892)
                     /locus_tag="ACG8OL_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4817278.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29498..29875)
                     /locus_tag="ACG8OL_001280"
     CDS             complement(29498..29875)
                     /locus_tag="ACG8OL_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817279.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30175..31056)
                     /gene="galU"
                     /locus_tag="ACG8OL_001281"
     CDS             complement(30175..31056)
                     /gene="galU"
                     /locus_tag="ACG8OL_001281"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4817280.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         9266 a         6455 c         5184 g        10153 t
ORIGIN      
        1 ttttttagaa atgtgatata acatccactt cagaatcaac gctgttaaat ttatcatgta
       61 tttcttcctt ggtcaactat aaattaagca ttactttgtg aaatgatttt tcgatcaatt
      121 attattattc gttatacaac cgttttcaac atattttgaa aataaaatgc actaaaaaac
      181 tatctttagc agaacatttc attgtatttt ttggaccaaa caataaaaaa agaagtttga
      241 aaaaaattca aacttctttg ttcgcttaat ctgtatttta gaagaaaggc actccctcta
      301 cttcgtaagt tccaagaaca gagcgcattt gacctggttc ttcccaaata tatccttcgc
      361 ctaattcttc tctttgattc ttttgttcag tttcaaagac aaacaacgga acattttcct
      421 ctttaatatg tgtatttgtc aattccatca aactaatctt gctaacatcg atatgaacta
      481 tttcttttaa gaaattaagt atattcgcta aaccagtctt tccttcagaa agttccgcta
      541 acgcaaattc caacttgtca tcaattgaat ggaccaaaaa ttttttagta ccatcctcca
      601 aatgcaacat aattgtacca gcaactcttt tgcttgccaa aatttccacc tcgatcaact
      661 tgttctatgg cttacataaa aattctagca cattttcaga aaaaatacac tttttatttc
      721 ttattttctt tccgttctag ttcagataat gcttcttcta tccaaatagg taactgctcc
      781 gcaatagtgg taaatccgat cttacgattt ttattcgtga aatacctttt tctgcgatat
      841 ggaacagctg gatttgtttt ttctaacgtt cttaaagata aactgatttt tttggaatat
      901 tcatcaatgt cgatgatctg aactgttact ggctgtccga cttttaacaa tgaatggata
      961 tttttcgtat aaccggattg tacttctgaa acatgaataa gtccttgcgt ctcgttatca
     1021 agagaaacga atgcgccata aggctgtatc cctgtcactt ttccttcaat tatttgtcca
     1081 attttataag tcatctactt ccctctcttc gcatttatca ttatactgta aatagctcaa
     1141 aaaaagaagc gacgaaaatg actggaggtc ttaagatgat cgaatttgat aaacagattt
     1201 caagaaaaga tacagaaagt gtaaaatggg acgcaattgc tgagacatat caaatggatg
     1261 acttacttcc tttgtgggta gcagatatgg acttcttgtc tcccggtggg gtagctaaag
     1321 cgtttagtga atacatcaaa catggaatct ttggttatgc cactctgtct gataaactat
     1381 atcaggctat cattcattgg gaacgccaac accatgctgt ggagttgacg aaagaaaata
     1441 tcgtttttac aagtggtgtg ttaaccagtt tagctgcagc agtacaaact tttacaaaac
     1501 caggcgattc tattttgatt catgacccag tttatcctcc attttcctct attatagaaa
     1561 caaatcaacg tcacatcgtt cgaagctctt tgctagaaaa aaataatcat ttcatcatgg
     1621 acttgtctga catggagaaa aaaatcgaag aaaacaatgt aaaagcaatg attttatgca
     1681 atcctcacaa tcctggaggg cgtgtttgga gcaaagaaga attgaaacag ttaagcgaat
     1741 tgtgtttaaa acatcaagtg attctgttta gtgacgagat ccatcaagat ttaactttat
     1801 tcgatcatac atttacttcc atgctaacga ttgatcctaa tttagatgat ctactagttg
     1861 cttttacttc cgcaaccaag actttcaatc tagctgcaat caaaaattca atggtattta
     1921 tcaaaaatcc agagatgaaa gctgattttg aaaaacattt agtgatgaac caacagcacg
     1981 aaatcaacac ttttggactg attggaaccc aagcggctta tgagacgggt ggagaatggt
     2041 taagccaact gattccttat ctagaaaaaa atgttcaaac agttaatcag ttttttgaac
     2101 agtatcttcc gaaagtgaga ataatgaacc ctgaaggaac ttacttaatg tggttagatt
     2161 ttaccgccta tatttcagac gaccaagaat tggagaacac tcttgttcgg aaaggaaaag
     2221 tcgtattaaa tcctggcatc acgtttggtc cttccggtca tgggcatatg cgcttgaata
     2281 ttgcttgtcc agaagaaact ttactggaag gacttaatcg aatcaagtat gctttagaga
     2341 caatcgatta aatcatttat tcaaaataaa aggacgagac cttactaggt ttcgtccttt
     2401 ttcataatta ctccgtacca taccaaacta ttctgaaatt tcaattgttt caatcactac
     2461 atcatgtaca ggtcggtctt gtggaccacg ttgtacattt gcgatttcat ctactgtgtc
     2521 catcccttct agtacatgtc caaagactgt atgtcggaaa tctagccaag gagttccgcc
     2581 acctttatac gcttcaatga tttctgatgg gaatccagca ccttctagtt gtccaagcat
     2641 attagctggc acatttgtgt tatttacaat aaagaattgg cttccgtttg tgtttggacc
     2701 tgaatttgcc atagataaag ctccgcgaag gttgaacagt tcacgactga attcatcttc
     2761 aaaactttcg ccataaatac tttcgccacc cattcctgtt ccagttggat cgccgccttg
     2821 gatcatgaaa tcaggaataa cgcggtgaaa aatcacgcca tcataataac cttttttgga
     2881 aagttcaatg aaattttgaa ccgtcttcgg tgctagttca gggaataatt gaacagtaat
     2941 gtcaccacgg tttgttttga taacagcttt tggtcctttt tcacttttta aatctagttg
     3001 tgggaatgcc atattgtttc ctcctaatat ttgcatactt cataataata ataacataat
     3061 acctaacctc tcgtaaaaat actagatttg ttttctttta tttttcatca ctagatacat
     3121 tctataactt accgtttcga tttcgaattc aaacattttt gagaagcatt ctcaattaaa
     3181 ttctgatata gtgtgaaaag ggaggaattt ttatggaaat ctatgacatt acaatcatcg
     3241 gtgcaggtcc tgtagggatg tttgccgctt tttacgcagg aatgcgccaa gctaaaacaa
     3301 aaatcattga cagtctacct caacttggtg gtcaattagc aactttgtat cccgaaaaat
     3361 atatttacga tattcctggg tatccagcaa taaaagcaag tgaattaatc gatcaattag
     3421 aaaaacaatt aactactttt aaccatacat tccatttaaa agaagaagtt ctctcgctta
     3481 ctcgtgaaga cgaagttatc gagattacta caaataaagg catccattat tccaaagcag
     3541 tcattttagc tttaggaaat ggttcattcc agccaagaaa attgaattta gataatgctg
     3601 aatcatttga aaaccacgga ttggattatt tcgtgaatga tttgatgaag tatgctggaa
     3661 aaaaagtcgc tattgctgga ggtggtgatt ctgctatcga ttggacgttg atgttagagc
     3721 ctattgcaag cgaagtttac ttgattcatc gtcgcccgga atttcgtgcg cacgaacata
     3781 gtgtatcacg cttgaaatct tcatctgtca acttgcttac tccctatctc atcgatggat
     3841 tatcaggcaa taatggggaa ctgacagata tccggttaaa aaaagtgaaa agtgacgaga
     3901 cgattgattt gatgattgat tctctgatcg tcaattatgg attctcttct aatttagaac
     3961 atctttcttc ttggggactt gatagtacca gaaatgccat tactgtaaaa tcagatatgt
     4021 ctacttctat tccaggcgtt tatgcagctg gtgacatctg tacctatgaa ggaaaagtga
     4081 aactgattgc tactgggctc ggtgaagcac ctactgcagt gaacaacgct ttacactata
     4141 ttaatccaaa ggaacgaact cagcctggtc acagcacaag cttatacgat aaaaatcttc
     4201 gtcctagttg attttttata tctctttgca tttcaagtta aacccgaaag cagtttcttg
     4261 catttttttc atgttccgtt aacatgaaat agagaaagaa aaaataaagg gggagcaata
     4321 atatggtcgt caaaactgag actttagaag aaaaggcaag agaactattg aaggaacgtg
     4381 gcgtaactgt agaagatatc gcacaacttg tcatgttttt acaaaaagac tatatttctg
     4441 atttgacttt ggcggattgt actgaaagtg tcaacagcgt gctaacaaag cgtgaagtac
     4501 ataacgccat tatcactgga atccaattag atatattagc agaaaagaaa aaattgatga
     4561 gtccgttgca acatattatc gaagatgacg aaggccttta cggtatcgat gagatcctag
     4621 ccttgtcaat cgtgaatgtt tacggtacga tcggctttac gaattatggt tatatcgaca
     4681 aggtgaaacc tggtatttta aaagaactaa acagccacga tggcgttcat gtccatacct
     4741 tcttagatga tatcgtcgga gcgatcgccg ctgctgctgc cagtcgatta gcacacaaag
     4801 aaccagaaaa aagaagtcgc cttactcaat aattcaacaa aaaaagaaag aaaacgtcta
     4861 tttagacgag ttctttcttt tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa
     4921 cagcaaaaaa taattctaat aatacaaaaa ataagataaa gctatgcatg aaaaattctt
     4981 ctggaagtac attgatgatt ggatcagtaa cgggatcgaa taaccagtca tcgttgttga
     5041 aaaacgtttc atgaaaaagg ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga
     5101 aaacaactgg cagtaacatg ccgatttgaa acggacgaat caaccgccaa agccgatcat
     5161 tcttttgcaa atatttgata aacaaaatac tcgggatcaa agtgatgaaa aacacagcat
     5221 aatccactaa aaacaaaatt ttcacttcat aaaagtgatg ggctccgcta gcagaaactg
     5281 ggaaatctgg caagctgagg atggttttga agggattatt gagataattc attaggtgat
     5341 caaaattttt cagtaacgtc tcttgatcta aagaagtata atccaaaatt tgcaaatgtc
     5401 cgatatcaaa tacgtatagt ggtcgaaagt tgatcgtcac accaactgcc agactgatca
     5461 gtgtcagaaa taggctgaac aatccagcat attccatcca ctgccatctc gaatttttca
     5521 tattagaagc tccattcatc cagtgattgt ttcacatagg tagctgggat aggcaaccca
     5581 ggtacatctt cttcttttgt aaaacctgat aaaacgagca aagtatctat attattgcgt
     5641 atcccagctt ggatatctgt ttcatagtta tctccaacca tgattacttc ttctttttgc
     5701 agccctaata cttcaacggc tttatccata atgatggctt ctggttttcc gatgtagatc
     5761 ggcggcgttt gggttgctgt ctctacaaaa gagatgactg accctgctcc aggaagaagg
     5821 ccgcgttcag taggtatatt cttatctggg ttcgttccaa tgaaagtagc acctttttga
     5881 atagcaagtg ttgctttaac tactttttca taggtcagat aattatctaa gcctaccact
     5941 acatagtcag gagcctcttc ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat
     6001 cctgcttctc cgataacata gacttttttc ccttttccat ctgctttcat aaaatcgatt
     6061 gttgccaaag tagctgtgta gactgtctct ggagctacat gaatgtcaaa ctcatctgct
     6121 agacgattag ctactgtttc tggtgactta gtcgtgttat tggtaacaaa caagaaaggt
     6181 aattttcttt tttgcaattc ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca
     6241 cgataaatcg ttccgtctaa atcaattaaa tatcctttat agtccattca tccactactc
     6301 ttcccttttt ctgatcgtaa atgctgtttt tgttttttct acttgtttag gttgttcttg
     6361 tttttttact ttttcagagg tttttgctga ctctgttttt cgatttttaa taaccggttt
     6421 tttgcgatta gatgcgactg gtcctttttt ctcatcgata tacgcacgat tatggttttt
     6481 tttctttttt cggcgctgag ttgtcttttc ttttttaccg ccgattctct cgatgacaaa
     6541 atacgcacaa ccaaaattgc aatattcgta aagatagtct tccaaggaat cgattcgctg
     6601 atctggatgc gcccgtcgat tatccgcttg gaaaaatcct tttaggcgta gttgttcata
     6661 accccaatct cctacgatat aatcatatcg ggctagtaca tcactgtaac gttctccaag
     6721 cttttctgca ttaaatcctt cacgataatc ggacacaagc cgataatgtc tttcacctat
     6781 caaaaaatta ctgtcatcta acagtgtcac tagctcgcct ttgacttttt ctgctttagg
     6841 ctcctcaacg atttcttcca aagcagctgt cagttcttcg gtgactgttg tttcctgttc
     6901 agctgatgat tttttcttag caatttctgt tttatgctca acgttctgct gttcattttg
     6961 ttttgcagac atgcgttcac cacctatctt ttttattata ccttgttttt atttgattgg
     7021 gtagtgagct ttcaagtaat ctcctaccac acttcggata aattcaggga acaaaaattc
     7081 attttttcca gcaatctcaa tcgttggaaa aaatggaaca aacatcaaat gatctacggt
     7141 agtaaaagta taatttttat tcagatcgat tggcttgttc ttccaaaaga cttgatggtt
     7201 aatagaatca tatgtgattc cattatagac catctgccca aaaatcttac cgcgaaatcc
     7261 cattcccttc ataggatagt tacgtaaaaa gttacgattt ttttctattt ctaaaactaa
     7321 gcggatcaga tcatttccac ccaatgtgac atttaacaag tgcatcggat gcggcaacgc
     7381 agtgtgaagc tgatcctgat tgacccgccc ttttggaatt tcagttaaaa acagaccact
     7441 gtttaatatt gctgcttccg tctttcctcg ttctttcacc gcctcaaagg ctacttggat
     7501 aaacgaatgc tcattgaaga tatctagact cagagaaaat ggcaaatctg ctacttcttt
     7561 ttcttttaat aattcatgcc cacgttctag ataactattt atttcttgtt catcttctgt
     7621 aaaagctgtc attgtttcgg tagggatggc tttggctgaa tgcttaagga tttttttctc
     7681 ttcatcgatg gttaaatgga cttcaccgac atactgcccg tactttccag cggctgctat
     7741 ttggacatca ttgattacgt gaccttctct gaataaatga tgggtgtgcg atcctaaaat
     7801 aacatcaatt gctggtattt cttttgcaat ttggaaatca tcctgaatcc cgagatgact
     7861 catcaaaact aaaacatcca cttgtgaacg taaatcttcg actaattctg gcaaaatatc
     7921 ataaggattc cgtatgtccc agccattcgg attataagta agcggaaatg gagcagtaaa
     7981 tgcaattagt ccgattttcg tctggtgttt cgttgtaatg attttatatg gtttcgtcca
     8041 tgccggtggc tggagcgtat ttttatcgaa taagttatcc aggatgatat caaactcagc
     8101 atgatcgtat aaatgattca actcttcttt ggaatttcct acgccttcat tgtttcctat
     8161 cgtcgctgca tcataaccaa cttcattcat cagcaagata ttttcctgtc cattcgttgc
     8221 ttctgacaat ggatgccaac gatccacaaa atcacctaga tctactgcaa tcgttgtcgt
     8281 tttttctttc ttttcgtttt ctcgtttacg ttgatccaaa aaccttctga tccttggcca
     8341 attttctaaa tgagaatgca gatcattcgt atgtaaaatc actatttttt ccatgtaccc
     8401 tctcgcctct ctttatcttt atcataccat aggcagcttt ttcttccatt gaaacaagct
     8461 ctatttcaga gtttttcctg ccttgaagca aattgtataa gtacaatatt cgataaatca
     8521 gtctggctaa aaaatcagga acattcattt ttacagtact ataaaaataa aaaaaacaga
     8581 cccgaaagtc tgttgtcgtt tcattataat cagacacatg gcggaacctg cttagtgctg
     8641 cttccttcca gacctgacac gcttcacaag cccaccattg tcctagcctt tcggcaaaga
     8701 atagtatacc ttatttttca ttcgttgacc agtctttttt taatttcttt tttcgctggc
     8761 ggagctttcg gaaaaagtca gtcaaaatca tcccacattc gtcttctaaa atgccacctt
     8821 ctacatatgc ttggtggtta aaccgctcgt cctccaaaag attcatcaag gtaccggctg
     8881 ttccgccttt tggatcatac gcgccgaaat aaacttctgc tacacgagac aaaatcatcg
     8941 cgccgctgca cataggacaa ggctctaagg taacaaaaag tgcagcatct tccaagcgcc
     9001 agcttcccac tttttcacag gcttccttga ttgcaagcat ttctgcatgt gtagtagcat
     9061 cttgcgtggt ttctctaagg ttatatcctc ttccaatcac ttttccatcc aatacaacca
     9121 ctgctccaat cggaacttca tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc
     9181 gcatccattt ttctttttct actgcatcaa gatttgtatt taccaattac ccttccctgc
     9241 ctttgttttt tcttgtttta ctttagcata tgataaaaag gctgtaacta actttttttt
     9301 ctatggtaaa ataaatacaa gaggtgaaca agatgaaaat ggataaatta cttgctcttt
     9361 acccacaagc acagaaaaaa aactctccag catcagaagc ggatatcctt tcttttgcaa
     9421 ttgatgatgc ttttatctgg atcaaacaag attcattatc tcaacaagaa accagccttt
     9481 tgaaagcgtt attccctgta atcaacgatc aaaaaaaaca cccatggtat cactatcttt
     9541 ttaaagatgc ttcttgtgtt caagaaaaaa gctttcgttt gatccagctt cacgtcgaat
     9601 caaaaggaga attcttaaaa aaagaatggc aagatactat tttggaatta tttcgctatt
     9661 ctgctgattt ctttttttat acagacacag atgcgctctt agtcgaacaa ataaaccgtg
     9721 attatcttga tgctactgaa atcaacggta tttttctatc tttagatgct gattttgata
     9781 cgacaacgtc tgtattcgtt ggttctttcc attcacctgg caccgacctc gtccctttgt
     9841 ttgctgaaga aagaaaaatt tttttaacag agcaaaacaa tctttatact cacagccgta
     9901 ctttttctat gcaagatgtg gctttacatt actatacaaa agatactgta aaagatagta
     9961 agttgatcaa aagttacata gaactgatca aacaaaatga aatggaagtg atcattcaag
    10021 aattatggaa tgaattaggc aatgtcagct ctgctgcaaa aacactgttt cttcatcgta
    10081 atactctaaa atatcgaatc gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag
    10141 ccaacgattt actgttttgc tatcttttat tgcttcatga acataattaa agatagatta
    10201 aaggggttgt gacaataatt ttgtcacaac cccttatttc gaatgaatag tgttcaaaag
    10261 ctagtttctc ttacatcatt ttttcaacaa aatgcattag ccggtctttt acatcttcgg
    10321 ttgaatcttt gattgaatca ccataaacag agattttctt ttttcctgat ttgatttttg
    10381 ctaacattcc catcaaagaa tccaagtcac tatctgaaag accatctacg atttctagta
    10441 atttttcatt tccatcaaat ttgtcataga cgaatttttt tactttcgtc cgagtagtca
    10501 tatgatggat cttttccatt acttttcccg atacaacgac tgctgtcgct acactggcag
    10561 cggctgctac gcttaatccg attgttactt ttgttgatgt tttcataagt atcagctcct
    10621 ttttatctat ctccatcata gcatagacaa aaaaataaac gaactaatcc actcagtgaa
    10681 atctcaatcc tttagggaaa aagttcttta ctgttccttt tacttgtttc ccaaatccga
    10741 caggtatctt atctaatact agcaaaaccc atccttgatt cccatcacgt tgaaatgttt
    10801 ctcctgctgt gtaagattgc cattcctttt gagtgatcgg caagcaaggt atattctcta
    10861 tcttttttgt agcaagcgct aatgcatagc taggttcaaa tcgattcttt ttgaaatctc
    10921 ctaaatgcaa acctgtacgt acgactttta aaccatcgag agaaggggct aactcaggaa
    10981 cttcccaaag atggtcgttg aacactaaaa gtcttccagt agcttcataa tgaaaatcat
    11041 ttgaaaactc cgtccagagt ttttcctgct cttttgtcat ttgcacttta cttttttttc
    11101 gtgtcttctt ttctttatgc atctgatttt gtccatgaaa agtcagcttt gcaacaaaat
    11161 gtccttcacc ctgatcttta tgaggccata tacgtattgt tttttccaaa ccagctacgc
    11221 ttccccattc agaacgtcct gaactaacgg attgagtcaa aggaatctcc tcaatcgtta
    11281 ctggataatt ttctacaagc cagctaatga tctcttcatt ttcttctggt gcaaacgtac
    11341 aagtcgaata aatcagttgt cctttatttt ttagcatttt tattgctgaa gacaaaatct
    11401 cttgctgacg tttttgacag tatagcggag attcttctgt ccactctttt attgcatttg
    11461 gatcttttcg gaacattcct tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa
    11521 agcccgaaaa atgagggaca agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa
    11581 ctccccatcg ttctatattt tctgataaaa tcttcgcacg tttcggaaag atctcattcg
    11641 tcactaataa ccctttcccc ttcatttgag cagctaattg ggttgattta ccaccgggag
    11701 ccgcacacaa atccaaaact ttttcaccgg gttttgctgc agcagctgtt ccaacgatca
    11761 ttgcacttgg ttcttggcta tattcatacc cagcttgatg gaggaaagac tttccattga
    11821 cagtgccaag aaatccctca ttgctgtagg gagcgggttg taattcttcg ctatggtaag
    11881 tttggaccat gtccaatcct gccggtttta gtggattcca ccgaaatcct tttttcacgc
    11941 ttccttgttc aagcgcagaa aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt
    12001 ttttgataaa ttgctgtggt aacgttgtcg cttcctcctt cattccaatg tttcgttaga
    12061 cattttacaa tcttttttac gaaaaataaa ggtggctgat ctactttttc atgttgctgc
    12121 ctaaggtgac tgttccttta agatattttc caagtattca tccgtcacct cgtttttatc
    12181 ccaaacagcg ttcatcattt caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc
    12241 caatgcatta agccatacca attcaagcga cttttcttct ttgatcttcg tgtgaccttt
    12301 gacatatgag aaaaatggcc gcttaaatag cgaatccatg tctaaatcag attttgcaat
    12361 gatttcacga atactttcat ggatagaaaa aaaatctttc aataagtaga caatgcttaa
    12421 aacatcatcg gaactgcatt cagctaagta gttttcaaaa taaacagtga tttcttctaa
    12481 tatcttctct tttaacccgc tgattgattt gaaatgtgta taaatcgggt atgtagaaca
    12541 accgcagaac tctgctaact ctcttgcagt aattgacttc attccatatt cttccacgta
    12601 taccatactt ttgaacagca aatattcttt actataattt gttactctcc ccatcattct
    12661 tacctccgtc tcttttcatc tacccataaa taaataacat aaataattct aaaaatatta
    12721 ttgataatta tcaaaacata tttagttata tccattaaaa atcaattttt ccgccttaaa
    12781 aacaagaata tcttgacaaa ataccctagg aaataaacaa tagaataaac aaggtaacaa
    12841 aaaaattaaa taacgttata taaaaaagaa ggcgacactc tgtgagaata acacagactg
    12901 aaatgacttt ccactcacag tttttgtcta actatataca aaaacagaat aaaaaggagg
    12961 tatttatgct aattgaagca agaaaaaaag agttacttgt tctacaaatg gtcattcaaa
    13021 aaaataattg ttctttacaa gaattatcca atgacttaca tattccaaaa cggacaatca
    13081 aagaactgat ccgaaaaata aatgtatcac tcaagcaatt tttattgatt gatgaattta
    13141 tttattctaa tcataaagga gagatccaaa ccaaagaaac cgatcaaact aaaaagttag
    13201 ctgttttttc taagttaaaa ttatgctacc taaaagaatc caatcgtttt aactatttga
    13261 tgttactaat caattatcca caaactgctg taccaaaaaa atatttactt gaacaacttt
    13321 atatatcgcc ctcctatctc gaaaaactga caaaacagct caatcattcc ttaataaaat
    13381 tccatttacg gatcctatct gtaaaaaatt gctgtactct tgaaggaaat gagttatcta
    13441 tccgtctcta tctttacttt tttctttcag atacatttgg aggtaacgat tggccatttt
    13501 cctcacttga attaaatgca ctaaaaatga acagtacaga tgagcaaata gaaaaaattt
    13561 caaaacaaga aaaaaatatc tacttgctgt taactttatt catattacga aacaaacaac
    13621 aggcatattt tccaaaaact tcttctgaaa tcactgaatt gatggactta ttgcagcata
    13681 cacatgatta ttcagaatac tttacttctt tttcagtgag tcgcccttgt gaagagacag
    13741 aaacagagag agcctacttt aattattttt tctattgttt ttttccaaat caaatctctt
    13801 ttcgacaaaa acaggagatt ggttttttat tttccacaag caaaaacctt atttgtcgac
    13861 aagtcggtca attgatgact tacttaactc aacagtttcc tctcaaccat ttagaaaata
    13921 aaataccaat ttatttttat tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg
    13981 aatccagcag tagtttttct acactttgct ttccagaagc cacgtccaat cattctagtt
    14041 cttttataca ggaaaagaaa ttaagagaaa aaatcgatac aattttttca aaaaatgggg
    14101 aaagaaacca aatcagtcaa taccttagtc aattgatcca acacttacta caaacagaaa
    14161 cgaaagagca gttactcatc catttgcaga taaccaaaca gtttacagga aattatcatc
    14221 ttcaaagcca tttgcagcag ttatttcgca aagatgctgt taaaattacc gcatattcag
    14281 aacatgctga tttgatcatt acagatcagc atgagaaaat ttcagatgaa acggagactt
    14341 tctatctgga tacaacgcac aatacagtgt tatttggcaa gttaataaaa gcggttcatg
    14401 aactgtatat gaaaaaaggt tatgagaaca aaacaaagag gatgtgacaa aattcttgtc
    14461 acatcctctt attccaaata aatggtattc aatcagctaa ctctcggtat tagctcagat
    14521 caaagcgctg atttcacaac ttctttattt taatctcttc ggttgccgcc gaacaataag
    14581 atcaagcgca acagctgcag gaatgtcgaa agcgctgctg caacataagt cagtgctgct
    14641 gcaaataata cgtgtcttgc catcggtact tcttcttccg tcagtagtcc gccttcactc
    14701 aaaatttgga ttgctcgccg agacgcgtta aattcgactg gtaatgtcac caattggaaa
    14761 agtaatgcta aagaaaaagc gaaaatcccg atattgatta acgtagagtt ccagctaagc
    14821 aatacaccta ccataatcaa ggggaacgaa atcatcgaac caaagttagc tgctggaaca
    14881 agtgctgcac gaatcttcaa tgggacataa cccgaagcat cttgtaccgc atgtccgcat
    14941 tcatgagctg ctacacctat cgcagcaact gaagtagact gtgcagttgc ttgagacaaa
    15001 ctcaacatct tattacctga attgtagtta tctgtgagat ctccggcaat ttgttgaaca
    15061 ccgacattat ttatctgttg actttgtaat atatattgtg ctgcttgtgt accagtcaca
    15121 ttgtttttgc ttctcacttg atcgtatttt cggaaagtac tattgacgta cgctgacgca
    15181 attccggaaa taactaatcc aagaatgacc aaaagaaatg tcggatctaa aaaaccatac
    15241 attccataac catacatttt cgtttcactc cttttcgttt actttatttt atcattagta
    15301 gtaggaaaag aatgtgaaaa gaccttgaat attctttaat tctttttcac aaattccgat
    15361 ttcagtttca tcggtccaaa tccttctacc ttgcaatcga tattatgatc gccttctact
    15421 aaacggatgt tcttgacttt tgttccttgt ttaatggcac ctgacgcacc ttttactttc
    15481 aagtctttaa tcactgtcac gctatcgccc tctaacagac ggtttccatt tgcatctaat
    15541 accactaatc cgtcttctat gacagtttct tgttcttctt tgttccattc gttcccgcat
    15601 tctgggcaaa tcaacaagcc tcgatcttca tatgtgtact ctgagccaca ttctgggcaa
    15661 tttggtaatg tcatcttctt tctcctttaa aatctttagt gagcactatt gtaacggaaa
    15721 aagaagatct tgaaaagata catttgtgat ttttactaca aaaggcacgt tttcgaacga
    15781 agtatcattc tgtttttctg ctcttatacg attggcatat atcttggatc tctttataga
    15841 tttcctctac ttctgctaaa tcttttgcat tcgcaccact tgctaatgga tggcctcctc
    15901 catgatggcg tttagccaat tcattgatga tcggcccctt agaacgtaaa cgtacgcggt
    15961 aataccctga aggctgctga acaaaaatcc cccaagccag cacttcgtca atcactccag
    16021 gaagtgaaac gatcgaagct gtctctgaat catcgattcc atatttatca agaatttctt
    16081 gcggaaggat tattcttcct gcaccatttt catccacctg gatattttga taaagataac
    16141 cggaaagttt agcgactttc atcggcattt gttcaatctc tctgttcaaa gcacttgcat
    16201 caaatggata atttctcaat atcgaagcaa tctctaatgt acgggacgtc gtggagggat
    16261 acaaaaagcg ccctgtatct ccgacgattc ctgcataaag taaccgtgca gccttaacgt
    16321 tcatctttaa agattcagga aacatcaaag caaattctgc aatcatctca ctacagctac
    16381 ttgcttttgt atttacccac acaagatctc cataagggtc atcattggga tgatggtcga
    16441 ttttgatcag tttttctgcc aactgatatc tttgatcact gattctgggt gaattagctg
    16501 tatcagtcac gatgactagc gctccttggt agaagacatc ttcaacttct tgcatttctg
    16561 ctaaaaagcg aagcccttca atcgtttcac ctacttgacg tatttctttt tctgggaaac
    16621 tttcccgtaa tagttcggct aatcctactt gtgatcctaa tgcatcagga tcagggcgca
    16681 aatgcctgtg gataatgatt ttatcgtatt tttttattgt atctactatt tcttgctgta
    16741 ctgtcatttc actcctgctt tctaagaacg ttccattact tgacaaacaa cgattgcttt
    16801 ggaaacgatg gtgttttcaa taaacacttc gatatccagt ttagcggaac gtctgccgat
    16861 ttccaataca cgtggacgaa tgtctaattc gctttccaat tggatcagcc gcaaataatg
    16921 taggttcact tgttcaatca agatatttcg tttttggttc atcaccattg ttttttgtgc
    16981 gacatcagaa atgatttcgc taagtacgcc aaaagaaatg gttccaacgc cattgaccat
    17041 ttgtggtgct actacgaatt tgaacttagg gttttccaaa cgatttcctt cacggtcagt
    17101 atcaatcgta tggatatttc cagatatctg atccgaaatc gtatctgcaa tctgcggttg
    17161 tcgctggaca agctgcatcg ctttcattac atcttgccgg gtaatcaagc cttccaatgt
    17221 caagtcatcg gaaacgaccg gcatgacttc tagcccatcc cagatcattt gatggctaac
    17281 agaggcaata ctcattgttt tctttacact gattggttct ttcgtcatca cccgatcgat
    17341 gatctggttt ggcgatttcc ctaaaacatc ttttgccgta acaatcccca tcaaacgatg
    17401 atggcggttg acgactggaa aacgcgaatg ctgcgtttct tctgacaaac gctgataatc
    17461 agaaatcgta tctgtctgct gcaaataatg agtcttttca gctggcatat agatatcact
    17521 gacaagcatg atatcttttt tgatcaattg gtcgcttaac gctcggttga tcatggttgc
    17581 gactgtaaat gtatcataag aggtgcgtaa gatcggcatt tcaagatcat cagctagctt
    17641 tgcaatctca gcagtcgtat tgaatcctcc tgtgatcaat actgcggcgc catcttccaa
    17701 tgctaatttt tggacttccg cccggttgcc gacgatcatt agtgatcctg gcgtaatata
    17761 gcgttccatg gcatgtttag tcatcgcgcc gatgacgaat ttattcaaga ccttgtctaa
    17821 tcctgccgcc ccgcctaaaa cgtccccttc aatgatcctg acgacctcgc caaatgtcaa
    17881 acgttcaata tgttttttta atttgcgttc gatacggatg gtccctactc gttgaattgt
    17941 tgaaactagt ccgacatttt ctgcatcttt gattgcacgg tacgctgttc cttcactgac
    18001 attcaattct tttgcgatgc ttcttacaga aattcgattg ccaacaggta aactttcaat
    18061 atattctaaa atcaaatcat gttttgtcgc cattttctgc ctacacttcc agcgtttccg
    18121 agacgctcat tatttttcct tcactatctt ccagtaactc tacaaaagat tcgggatctt
    18181 gttcaatgac agggaaagta ttgtaatgga ttggaactac tttcttggca cgaattgttt
    18241 gcgcagcctt tgctgcatct tctggcccca tcgtatagtt atcgccaatc ggcaagaaag
    18301 cgacatcaat atcgaattct tctgcaagca atcctagatc actgaaataa gcagtatccc
    18361 ctgcatgata aatcgttttt ccttctgctt gaataataat tccgctaggc tcgcccatat
    18421 actgaacgat accgtctact tcatagcttg aactatgctg tgcatgtacg aattttacca
    18481 cgccgaatgg aaacgcatgt tttccaccga tattcatacc gaatgaatct actcctctgc
    18541 tgttcgcata atcagcaatc tcaacaatac tgataattgt ggcttgattt tcttttgcaa
    18601 taggaatcat atctcctatg tggtcactat gaccatgagt aatcagcaac caatctgtct
    18661 ttacttcttc tgcagttaag tctgtcaatg gatttcctgt aataaacggg tcaatcatca
    18721 cgcgttggcc attttccatt tcaattgtta aacaggaatg tccatgatac gtaattttca
    18781 tattttacac ctcgcgtttt ctctgtatct tttttattaa aaacgatttc atcacttgca
    18841 catcaatgaa aaaatatagc tgctaaatat attttcattg gattgatagt acagtatgta
    18901 caaagcaatt gtaacagata atacaggatg caccaagtac caagcagata taatctcaag
    18961 tttgaggtct ctgctctgct tagcttctct tctcgtccct taactaatta aatcagcgta
    19021 aataggatgg acgatctttt ctaaatcggc tacttttatc cgaaggcttc tcccgatttc
    19081 accgcttgac acaattactt cttctttttc ttttaaagaa ctgtctgcta aaaaacaata
    19141 atcaggatgt gcgagtttta ttccaatagg ggtgtttgct ccgtggggat atcctgtatg
    19201 cattaataca aaatccattc caggaaaacc aactttacga tctttcgtca cttgtctcgt
    19261 ttttttgtaa tctaagtgat cgttcaaagg aatcaatgcg atgatcaccc cagttttgtt
    19321 tcctttcact acgagagttt tacatatcaa agctggatca atccctcttt tttgtagttc
    19381 ttctgtgaaa gattcttcta ccacatgact aaaatcaagt ggttgatagc tgatcttttg
    19441 ttcttctaaa taacgttcga cttttgtttg actcatcatt gattcactcc ctttattcta
    19501 tcgtttatta taaagcgagg gatgctgtat tcaaaaatta tttgtttttc gcaaaaaaag
    19561 agcaagacga gcactctata gagaggttca tcttactctt tttgtttatt ttttatttcg
    19621 acgttgagta gcttccataa tgactggcaa gatcactggg cggcgttttg tttgttcaaa
    19681 taagtaacgg cctaattgat ctcggatgtc ttgctttaat ttgctccact caaaatcatt
    19741 gctttctaaa tgtttctcaa cgattgtttc aatcatattg ctgctttctt tcatcagatc
    19801 acggctagct tttacgtaaa cgaatccccg tgaagtaatt tgaggttttg aaatgatttt
    19861 cttttctcgc cgactgatag tgacaaccgc tacgaaaatc ccgtcttctg acaagatttt
    19921 tcgatcacgt aaaacaatgt ttccgatatc acctatgcct aatccatcaa tcattacatt
    19981 ttctgcactt gtagttcctg caacactcat acgcttgttc ttatattcca gcacgtctcc
    20041 gcgtccagta atatagatat ttttgtatgg catccctact gcatgagcca aatctgcatg
    20101 agcagctaat tcacgatact ctccttgaac tggcacaaaa tatgttggtt tcatcaaatt
    20161 gatcattaat tgcaagtcat taggattggc atggccagaa acgcgcatat tctcagagat
    20221 caacttcact gttccaccag ctcgataaat gatatcttcc gttttcgcca cgatggtttc
    20281 catcgcgatg cttggcgtag tagtgatata aaccaaatct ccttcttcaa tcttgacatt
    20341 acggtgtgta ccgtttgcca ttttttgcag agacttgatg ggttcaccca ttcggccagt
    20401 ttctaaaatg atcagttctt ctggatcata gtttttcatg tctttcaacg taacaagcag
    20461 atcttcactt ggtaattgaa gtttttctaa acgcatcgct gtgcgaataa ttcgttcgaa
    20521 atcttgacca gtcaagacaa cttttcgttg tgctttttct gctgcatcca atacttgttg
    20581 cactcgttgc aaattacttg ctacacaagc gacgatgata cgcccttccc aatatttgat
    20641 cgtatcaaat acttcatctg ctatttgggc ttcagaagct acaggctgag gattttctgc
    20701 atttgccgat tcacttagta aggctaagac tccttctttg ccgatttccg ccaatcgtgc
    20761 ataatctgtt tgatacattg ggatagccgt ttggtcaaat ttaaaatctc ccgtataaac
    20821 gatactacct tcttttgttt tcagattgat ccccactgaa tctggtatcg tatgagtggt
    20881 acggaaaaag ctcactactg tttcaccaaa gtctatttct gtatgctcat ctaccacatg
    20941 gaaatcctta aatcctttgg ctgcttcatt acgactgaca ttcaattttg ctaactcgat
    21001 cgtcaactct gtcccgaata ctggtacaga aatttttgac aacaagtaag gaagtgcccc
    21061 gatcgcatct gcatgtccat gtgttaaaaa gacaccagct acacgatcaa tgttttcttc
    21121 taaatacgta aaatcaggga ttacgacgtc gatccccaac aactcatttt caggatattt
    21181 tagcccacaa tctaaaataa aaatatcctc gcccacttct gcgacataca tgttttttcc
    21241 attttcacga acgccgccta atgggataat tttaatggta ctcacaaact tcacctcttc
    21301 ttatgacatc caagtttaaa gatgtcgatt ttcttcttta tctaaatata tgagccattc
    21361 caatgaagca tatgcaaaat cactatttct caacttatgg agaatcagca ggaaaattac
    21421 cagcacatcc gcgttctgac agttggatgg gcattttatt ttggatatac ttcattaaca
    21481 aaatggatct tttgcgaaac gctatagcca gaatacttat ataagtattc agctgttccc
    21541 cattattata caacaaataa actaaatcgc tagtatcagc aattgttaga acaactatgc
    21601 acgtaaaaat ccctctgtag catctaggat ttcttttgct attcctcttt catttcttct
    21661 tcgtcggttt gaaacatact tttttcattt taccacattt tttgcaataa ctatagtgac
    21721 tttttatata tacagaacct catgttgatg gtgtgcaaac agcataagat ttaagctagc
    21781 tttaagatag gttgtctatt atcaaatgca tctactagga gggatgcaaa atatgaattt
    21841 tatgaaacgt gcatggaaaa gtacaaaagt aaaatgggga cgttcattgt tattgtttgc
    21901 cgtgttcaca gccattctta tttttgtgct agctggcctc acgattcgaa gcgctgcaga
    21961 agaagcagcg aatcaagcac aaaaagaagt tggagcaaca gtgactttaa gtgccaatcg
    22021 agaagcttca tttaaaaaac aggaacaaac ttcttcaagc ggttctacca ctgaagaacg
    22081 tccagatcca ggcagttttt ctcttacacc agtttcctta acggacgcgg aaaaaatagc
    22141 tgaattagat aatgtaaaga gttattcctt tgaaagttcc gcctcagcta tggcaaaaga
    22201 tggaataact gccatcacta gttctgattc aagtgaagac agttcagaaa ctactgatgc
    22261 aggcggtgaa cctgctggag gaatgggcgg gaatccgcaa atgactcaag cagactttca
    22321 aatcagtggt gtgtcagcaa ctgcacaaaa tagtaatttt tctgatggaa cagcaaaaat
    22381 cacggatggt gaaggaatag atgacagcga caaaggcaca aacaatgtag tcattgattc
    22441 tactctagca gaaacaaatg acctggcagt cggagatact ttcacaatca cgagtactga
    22501 agatgaagat accacttatg aaatgacgat caaaggaatt tatgaatcca gcgaaacaag
    22561 cggcagtatg ggtatgaaat tcaacttcat gaatccgtcg aatacacttt acacttctta
    22621 cacttttgca aatgaactaa atgggacatc tgacgataac acgatagatt ctgctattta
    22681 cactctatct gatccagata aaatggatga gtttctcaaa gaagctgaga agctgatcga
    22741 cacagataca tttagcttac aatcaaatga ttccatgtat cagtcaatgt tggaaccttt
    22801 aaacaacgtg gcaagttttt ctaaaaatgt tgttttacta gtggctgtag ccggtattat
    22861 catacttact ctgatcatta tgattaccat tcgggagcgt cggcatgagt taggcgtctt
    22921 gctatcttta ggagaatcca gaagtaaaat cattctccaa ttgtttaccg aagtggctat
    22981 ttgtatgatt cttgcattag gagttgcttc gcttagcgga aacgtcgtcg cgaatgccgt
    23041 gggacagcag ctacttgacc aacagacaga aaccacgaca caaaaccagc aagctcctgg
    23101 cggtggacaa atgccaggta acagacaaga acaaggcggt caacgcgatg gtcaacaagg
    23161 aaccacgaat aatccatttg ctgtttctga tcaagtcagc gaattggaaa tcaatgtcaa
    23221 acctgcacaa ttgggactat tagccggagt agcatttgga atcagtctgt tttctgtctt
    23281 tttagcttct atagggatat taagattaaa cccaagaaaa atcctattaa actaaaggag
    23341 gaatttgatg ttacggacag aacaattggg attttggtat caagatccgc aagatgcgct
    23401 ttataaagat gtagaactga atttcgaagc tggaaaaatg tacgctattt taggagcaag
    23461 tggttctgga aaaacgactt ttctttcgct tatttctgga ctagatgcac caaaagaagg
    23521 aaaggtatac tatcaagatc gttcactgaa caaaatcggg ttaagaagtt atcgtaaaaa
    23581 tgatgtctcg attattttcc aagcttacaa cttgcttcct tatttatcgg ctctagacaa
    23641 cgtcatagca gcaatggaaa tcactaagac caagcaagag aattatcgac aagttgcttt
    23701 agatcgactg gctcgtgtag gaattgatga aatattagca aaaaaacagg tgaccaaact
    23761 ttcgggaggt cagcagcagc gagtcgctat cgttcgtgct atctgctgtg aacatcaact
    23821 aatcgtagcg gatgagccta ctggaaatct agatgaaaca acttcacaag atatcgtcaa
    23881 acttttccaa gaaatcgccc atgaacaaaa tcgctgtatt atcatcgtga ctcatgagat
    23941 tgaagtagca aaaagctgtg atgaagtctt tgaactaaaa aataaaaaat ttcaaaaaat
    24001 cgaagtttaa aaaacaggat aatgatcaaa taaaaaatcc gaaccatatc tgtctcgcag
    24061 atatggttcg gattttttat ttgcttgatt caattagtaa taagtggttt ttattttttt
    24121 tcgtcttttc gagaaaactt gtctttgact tcgtcgccta aatttcgagc tttttctttg
    24181 atgtcagcac ctagtgattg tgctttccct tctgtttctt tgctcttgtc gtctgttgct
    24241 ttgccgtatg cttcttttcc ttcaccgact actttatctt tcatatcttt agaacgtcct
    24301 tttaaatctg ccattgtgct tcctcctctt agtttgttta ttgatctacc ttcagggtaa
    24361 catcaacaac agatacagac aaacagaacg ctcgttttct aaaaaagata gttagagctg
    24421 ttgattaaaa acttttttcg aaagtccccc atactttgac ttctttttct tggaccatta
    24481 tttctccttt agcgataact gtatcaatat caagcgtttc tttatccaag atacacagat
    24541 ctgcatcagc accaatttcg atttttcctt ttttatccaa tttcaatata cgtgctggat
    24601 tactggttat agctcgtaag gcgatttcca aagggatact ttctttttgc acagcttctt
    24661 tgatccctac tagcaatgcc ttggcacttc ctactcctaa ccctaggaaa tgattgtttt
    24721 catcaaaata aggcaagctt ccttgtccat ctgaggtcat cgtgaaatta tctaaagaaa
    24781 catcttcctc gatcaatcgt ttcagtgctt tgctgaagcg aacttcacca tctgtcttct
    24841 cccaaaaatc aggatcttcg ctagcagtga agtcaatcgt gccaccacgt ttggcaaatg
    24901 ccacgcaagc ttcaaataat tctggtgtgc gatttgcatg agtaggcaaa aattgtgtga
    24961 tcggaatttc tgtttcttct acgattcgtg tcaatagctc tagctttctt tttcctccgc
    25021 ctaagtggac attgatgatc ccagcttttc cggataacat gcctcctaca cgagcatctg
    25081 ctactgcacg agcgaactct tcaaaacttg gctgagaaga acggtgatcg gatactgcga
    25141 tttctccgat tccgataatt ttatcaattg tcaaaaagtc tttgatgata gaatctgtta
    25201 ctgtttttac cggcaatcgg tacgaacctt cgtatacata agtggagatt ccttctgctt
    25261 ccagaccttt cgctttgctg atcaacgcag tcatatctct accttctccg tctgttccca
    25321 aacaaccgac aactgtcgtt acaccagctg tagtcaattg gctcaatgtg acttctggcg
    25381 ttcgattttg ataaccgcct tctcctcctc cacctaaaat atggaaatga cagtcaatga
    25441 aacccggggt tagtactttt ccctcacctg aaagttcttc tacttccact ccttcgtagc
    25501 cgccttctag ctgttcttct acagccaaaa tcttcccacc ggcaatcagt acatccattt
    25561 ttcctaagta atctggtgca aacacttcaa tttgtcgaat gattttcata catttttcct
    25621 ctttctttaa gtataactga tgataggatc ttaaaaaata atccaaacga taggatcttt
    25681 actcctttga ttttaccgta aaatgacggt tcttggagtc cttatctact gtttggatta
    25741 cttttttatt tacaggtctc cagtactact gcagacctat tgcaatggca atcatgatga
    25801 aaatagttcc taaaacaaac aggaatcctt gcatcttgat ttggaatctt gcccattttg
    25861 cccattcgat ccgtgcaaca cctagcacac caatcaagct ggctgatacg ggtgtaaaag
    25921 cgtccataaa acctgaacca agctgataag caagaactgc tacttgtctg gatacaccta
    25981 ctaaatctgc cagtggagcc atgattggca tagtcaaagc tgcttgtcct gaatttgatg
    26041 taacaaccaa gttgaacaaa ctttggaaga tgtacatcgc ccaagcacca atgacagctg
    26101 gaacaccggt caacgccgtc ccgatcccat gcaaaatcgt attcaaagct gaaggaacag
    26161 atgcatcaga tcctcccaag acaagaagaa tccctttggc catacctact acgattgctg
    26221 taccagctaa atcagctgca ccagattgga aagaagaagc gatgtcattg actttcattc
    26281 catctaattt gaaaagaaca gcaatgattc cagcaactaa tcccatcaca aagaattgag
    26341 aagcgatttc tggaatgtaa tagccttttt gagtcacgcc ccaaacgatc cagacaagga
    26401 caactaacat ctctaataag atcaatttat gcccaagcat gaatttttta ttttcatcag
    26461 atgtcttttt gatatgagaa cggaaataat catctgattt gtaagtcaac gaactttctg
    26521 gttttttacg gattttttct gcatatatca tcatataacc agcggctaat gcagtaacaa
    26581 ctacccacat aatcaatcgg aatgtcgcac cagacaacac ggggattcca gcaatccctt
    26641 gagccaccgc tacgctaaac gggctcatcc aagaggtcgc atttccgact tgagatgcga
    26701 cataagtcac ggttactgct acgatcgagt catatcctaa cgcaatgaca aatggaacca
    26761 tcaccattga gaatggaatt acctcttctg ccatcccgaa tgtagcgcca ccgaatgaaa
    26821 aagcaaagaa taatagcggt aaagctaaac gctccagtcc cttagtttta ctgataaacg
    26881 catagatacc tgcgtcgatc gctccggtac gcataataat accaaaagca cctccaacaa
    26941 caaggatcaa tgcagcgatc cctacagcag atccatactt atctccagat actagtcctt
    27001 caaaaacaaa atttaagaaa ccaaagccgc caaaatcatc tgttccccat attttggctg
    27061 ttttatgcaa ttttttagaa gtatcataga tattttcgcc gtactgatcg taaagcacat
    27121 catctgtcaa ggagatttca tctagttttt cctgcgtaag atttttttct ccgtcagcaa
    27181 tgactttttc caatcctttt tccggaacat ccagtttttc acgttctgct ggatgatctt
    27241 gcagttcttc taattgatca aacacatacg acttatccag ctcgtaagca taacggaagg
    27301 aatcttgacg taaaacagta cgagtacttg tttcgccatt tgcatctttg tattcaatat
    27361 cttgcgtgct gaactttcct gctggaacga caaaagtcag tatccatgca aagatgacta
    27421 caccaaaaat aatgacatac gtatggggtg tcgataattt agtgaagtcg cgtttcggtt
    27481 ttcctgaaga atttgttttc ttcaaattta tttcttcctt tcttttttca gtaattttga
    27541 tgaataaaaa atggataatc aaatgaatta tacagtaaaa atgacatttc cacatgaata
    27601 atttattaga aaatcacttt tttctatttt gttttttttc tcctatgtat gattattttt
    27661 ttctctttcc atttatatta agtattttat ttctctgttt ttccttaaaa ttcttttttt
    27721 atttttagat tagagaaaat ctcgtcctgt ttttttcatt ttacaatcat tgaaaaattg
    27781 aatttcattg caaaaaaaat aataaacact tataaatatc cattatctat ccttcaattt
    27841 ttgtgtattt tatttctatt agaaaaaggt atcctgttgg acaattttgt tatatcaggt
    27901 aaacttcatt gcaataatga taacaaatgt actataatta ctttgtaaag agttttgggg
    27961 aagactcttt catcaacgtg tattaagaga caagaaagaa tccatatgga ttctttcttg
    28021 tctctttttc cactactttt tatttttctg acactacata ttcttataac tcatttctca
    28081 taactgtgaa acaacttata aaaataacaa actttaaaca aaacaagctt aaaatcaaag
    28141 atgatccatc ttaaaataac taaaacaaaa aaagtagtcg ttgttaatcc tcattaataa
    28201 tcgttttctt tttttcaaac gctccatttt tatgtggaac attatttttt taaggaactt
    28261 tgtcaacatg tggataacta ttttccactt gtgtcctgga ctagctgttt tgctgtaaaa
    28321 tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg ctgttccgca gatcgctgga
    28381 tctagatttg gcaaagtcaa gactgcatca tgtaaattgg cgtggttgtc caaaaccaaa
    28441 tcagcaaatt caaataatcg caaaccactg ctatgttgcg atttgatact tcgtgacaaa
    28501 tcaaatccag taacaatgat taatggatga ccattttctt tgatccactg agctagttcg
    28561 atgattgccg ggtttcttcc ttcattcgaa agaagaagaa aaatgtcttc tgatttgaca
    28621 gaaagctgat caataattgc ctgtcctgca ccttctagcg tttcatagac acctccggct
    28681 ggattttcaa tcaatatact tggtacaaga tccggggttt gatcccaaaa ttcaaatgct
    28741 acaccactta gtgtgcggct agcatatatc tgcaatctac caccttgatt aatttgtttc
    28801 gcgatccaag tgccagcttg aagcatcagc ggctcttcta tccttaattt ctcttccaat
    28861 tggtgataat agtcaaaata acataaactc atttatctct cccgcctagc tatacttgac
    28921 atgaatagaa acagctgcct aatctaagga agtacataaa aaaacagtag ttagacagta
    28981 acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat tgaacgaata cgcccaatac
    29041 cttgacatag caaataaaac aacgtgcaga ccatggaaaa tatctacata ggggtagaat
    29101 tttcacccta gattaggtac aataaattag gtcgatgcac tgcattgact atggcaaaat
    29161 gatcggtcag ggtgtatgtg tgtgactggt cattccctga ttcttagcgc caactaaggg
    29221 tcagggagcc tttttttatt cagctgtttc tatctgaatt tattgtaagt aatctcttat
    29281 ccaagtgcaa gcaagaaaat acaaaaaaac attggtatga tttatatata tataaataac
    29341 ataaaaaaag gaatggctta tcccgataca acaagcattc ctttaaaaaa gattctttta
    29401 tataagtgaa aaaagctttt ttccaccaat taaataaatc tctcatctca atagtttatc
    29461 cattatttta tctttttttg cgtataccta ctaaatatta ccgtcttctt ctcgttcttc
    29521 tactttgttc gagagaatct gtactagaaa aagtagttgt atgtctacct tttctgccta
    29581 tccgagactt tcttttcata tatgggatat aaatgattag atacagtaca gcaaaaaata
    29641 aagcaaatac gcaagcttcc caactaccta acgccaattc aaccatcaag gtagcaaccc
    29701 caacggatgc tgcataccag ataaaaatca gaaacgtttc tttttttctg accatgcgaa
    29761 acatgataaa tagactaata agcaatagtg gcaacacaaa tatccaattg cttccttcca
    29821 tcagccactt gagtatatca agcagcgact gtgcaacagc gactaatttt cccatagtga
    29881 acacacacct ttttttataa ttaaaaaatt cgccttttta ttctactatg aatcttctgg
    29941 cttggctttc atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa
    30001 ccattagcat aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc
    30061 acgactcaac tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag
    30121 ctgccgcttt aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact
    30181 ttctgttctt agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc
    30241 cgaatgttga agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc
    30301 gtatcttttt cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag
    30361 tgcatccgtt agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa
    30421 tattttagga gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat
    30481 ttcaggtttt tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg
    30541 atcaataatt ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga
    30601 agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat
    30661 caaatcatcc cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag
    30721 cacggcatcc cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt
    30781 cgtttcttca actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa
    30841 ttcgatattt gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa
    30901 gatgtcttcg attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc
    30961 cacgattgga agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag
    31021 cccagcggca ggaatgactg cttttttgac cttcactt
//
LOCUS       DAZUJO010000026        30743 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000026 DAZUJO010000000
VERSION     DAZUJO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.026"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..253)
                     /locus_tag="ACG8OL_001282"
     CDS             complement(89..253)
                     /locus_tag="ACG8OL_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817281.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            548..1876
                     /locus_tag="ACG8OL_001283"
     CDS             548..1876
                     /locus_tag="ACG8OL_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS4817282.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2078..2911
                     /locus_tag="ACG8OL_001284"
     CDS             2078..2911
                     /locus_tag="ACG8OL_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS4817283.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2927..3664
                     /locus_tag="ACG8OL_001285"
     CDS             2927..3664
                     /locus_tag="ACG8OL_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS4817284.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3819..4787)
                     /locus_tag="ACG8OL_001286"
     CDS             complement(3819..4787)
                     /locus_tag="ACG8OL_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS4817285.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5019..5852
                     /locus_tag="ACG8OL_001287"
     CDS             5019..5852
                     /locus_tag="ACG8OL_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS4817286.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5886..6725
                     /locus_tag="ACG8OL_001288"
     CDS             5886..6725
                     /locus_tag="ACG8OL_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4817287.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6751..7311
                     /locus_tag="ACG8OL_001289"
     CDS             6751..7311
                     /locus_tag="ACG8OL_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4817288.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7510..9231
                     /locus_tag="ACG8OL_001290"
     CDS             7510..9231
                     /locus_tag="ACG8OL_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS4817289.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9396..10538
                     /locus_tag="ACG8OL_001291"
     CDS             9396..10538
                     /locus_tag="ACG8OL_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS4817290.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10554..11765
                     /gene="thiI"
                     /locus_tag="ACG8OL_001292"
     CDS             10554..11765
                     /gene="thiI"
                     /locus_tag="ACG8OL_001292"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS4817291.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12338..12985
                     /locus_tag="ACG8OL_001293"
     CDS             12338..12985
                     /locus_tag="ACG8OL_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS4817292.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13114..13330
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13378..16023
                     /locus_tag="ACG8OL_001294"
     CDS             13378..16023
                     /locus_tag="ACG8OL_001294"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS4817293.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16333..17646
                     /locus_tag="ACG8OL_001295"
     CDS             16333..17646
                     /locus_tag="ACG8OL_001295"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS4817294.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17636..18289
                     /locus_tag="ACG8OL_001296"
     CDS             17636..18289
                     /locus_tag="ACG8OL_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4817295.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18344..19036
                     /gene="radC"
                     /locus_tag="ACG8OL_001297"
     CDS             18344..19036
                     /gene="radC"
                     /locus_tag="ACG8OL_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS4817296.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19186..19386
                     /locus_tag="ACG8OL_001298"
     CDS             19186..19386
                     /locus_tag="ACG8OL_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4817297.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19640..21319)
                     /locus_tag="ACG8OL_001299"
     CDS             complement(19640..21319)
                     /locus_tag="ACG8OL_001299"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS4817298.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21321..21533)
                     /locus_tag="ACG8OL_001300"
     CDS             complement(21321..21533)
                     /locus_tag="ACG8OL_001300"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS4817299.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            21926..23656
                     /gene="efrA"
                     /locus_tag="ACG8OL_001301"
     CDS             21926..23656
                     /gene="efrA"
                     /locus_tag="ACG8OL_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS4817300.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23653..25413
                     /gene="efrB"
                     /locus_tag="ACG8OL_001302"
     CDS             23653..25413
                     /gene="efrB"
                     /locus_tag="ACG8OL_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS4817301.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25508..25705
                     /locus_tag="ACG8OL_001303"
     CDS             25508..25705
                     /locus_tag="ACG8OL_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817302.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25858..26343
                     /locus_tag="ACG8OL_001304"
     CDS             25858..26343
                     /locus_tag="ACG8OL_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS4817303.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26447..27040)
                     /gene="clpP"
                     /locus_tag="ACG8OL_001305"
     CDS             complement(26447..27040)
                     /gene="clpP"
                     /locus_tag="ACG8OL_001305"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS4817304.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27220..27747
                     /locus_tag="ACG8OL_001306"
     CDS             27220..27747
                     /locus_tag="ACG8OL_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817305.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27838..28575
                     /locus_tag="ACG8OL_001307"
     CDS             27838..28575
                     /locus_tag="ACG8OL_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS4817306.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28579..29496
                     /gene="miaA"
                     /locus_tag="ACG8OL_001308"
     CDS             28579..29496
                     /gene="miaA"
                     /locus_tag="ACG8OL_001308"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS4817307.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29498..30727
                     /gene="hflX"
                     /locus_tag="ACG8OL_001309"
     CDS             29498..30727
                     /gene="hflX"
                     /locus_tag="ACG8OL_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS4817308.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10151 a         5259 c         6561 g         8772 t
ORIGIN      
        1 tttcttttat tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt
       61 acgaaagagt agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga
      121 aataaatcag cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat
      181 tcccaaagtt atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa
      241 tcaggttttt cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga
      301 gtattttgaa agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg
      361 ccattttcgt tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg
      421 acatttttct tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa
      481 gagattgtat ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga
      541 tgattggatg aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc
      601 aaatatcaaa cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc
      661 tgaaaatgtt tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg
      721 gaaagtggct aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt
      781 ccgtcataag atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac
      841 ttttttctgg gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg
      901 aaaagaggca atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg
      961 cggccaatgg gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt
     1021 gatgccggaa agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt
     1081 actgcgtaaa gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc
     1141 tattgaagaa aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt
     1201 aggaacacct ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct
     1261 tgaccgagga ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga
     1321 ttatgttagc gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt
     1381 agagatgcta ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga
     1441 aactttcaaa aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg
     1501 tgatgtaggt caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat
     1561 ggatgatcta tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga
     1621 aagtctgatg acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac
     1681 aaaatggaaa gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat
     1741 gagtgatgcc tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac
     1801 gaaagagcaa ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat
     1861 gggtgcttta gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct
     1921 ggcttaagcc agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc
     1981 tataattcta aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg
     2041 acgaagtaga ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc
     2101 tgatctgaaa ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta
     2161 tcgtataaaa accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg
     2221 attttttgaa gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga
     2281 tacgtatatg ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc
     2341 gggaaaaggg gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc
     2401 gccacaattt ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt
     2461 tgaagaagat tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc
     2521 agaagaaacg aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg
     2581 tgtattgaaa tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg
     2641 gagtggtaat gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc
     2701 ttatgggaat cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga
     2761 atttttagat gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc
     2821 gatttaccag ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc
     2881 ccaagtagat caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata
     2941 taccaaaaag catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc
     3001 ttcgatgtta ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg
     3061 cagtgaattc gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat
     3121 taatcgtctc cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa
     3181 taagtttttc tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa
     3241 aatagaatct gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga
     3301 agagatcatt gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat
     3361 tgaaaagatt gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag
     3421 taatcagcat gttaataatg cgatgtattt taattggatc attgacgtat taggatatga
     3481 ctttttgact acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta
     3541 cgggaatgag atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa
     3601 acatgaaatc cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa
     3661 ataaaaaaga ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa
     3721 attgtttctc aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac
     3781 agcctctctt tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc
     3841 aattcttcac ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga
     3901 ctattaatac tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca
     3961 taatcataaa caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt
     4021 ttttggacaa gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct
     4081 aaagcttcga ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct
     4141 tgcatcccag catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt
     4201 cgctttgttt ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg
     4261 ggacccaatg taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag
     4321 cgtgcagaat ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca
     4381 caacttgcga cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta
     4441 cttgaacgca ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga
     4501 aaaaaagcag tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct
     4561 gatgcataag cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt
     4621 ggtgagggaa gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt
     4681 tctgcatcta ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct
     4741 ttcatggcga tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc
     4801 ttacgaataa ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct
     4861 atcagtgaag aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg
     4921 aaaagactca accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag
     4981 aaagcagcca aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat
     5041 cgtttaaaga acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag
     5101 ggtttctacc cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag
     5161 ttgagaattg gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag
     5221 gaatcagcgg tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc
     5281 attttttaga tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc
     5341 aattcccctc atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa
     5401 agtggtttga gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg
     5461 aatttcttga acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg
     5521 atgagccaca gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact
     5581 ttgctctttt tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt
     5641 ggcgtaaaaa aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg
     5701 aagtcaaagt agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg
     5761 gtggtgatgc ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg
     5821 gactcaatcc aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga
     5881 agtctatgat caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa
     5941 aagcgttgga atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg
     6001 gtgtagcaac ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag
     6061 atatgtatgt cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc
     6121 acccttttga acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac
     6181 aaatcgtttt tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat
     6241 cgatttttat caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga
     6301 aaaaaatcct ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga
     6361 tattaaaaga accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt
     6421 tagaacatcg ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca
     6481 tcccaaaagg tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg
     6541 aaatggaaga aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg
     6601 taggaattgg agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg
     6661 tgactcaatc aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt
     6721 attagtcaat gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta
     6781 gaaacattcc acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa
     6841 aatgaagctc atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct
     6901 acttcaccta ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat
     6961 aaagcagaag aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa
     7021 gtagatgcac tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata
     7081 gtgcctgatc gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat
     7141 ggaaaacctc attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg
     7201 gaatatgctc cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt
     7261 gctaagaaga aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt
     7321 cactattttt gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa
     7381 tgcatgaaaa tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat
     7441 ttggatttat gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag
     7501 gaaccataaa tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc
     7561 attgtcgtct ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc
     7621 ttagaaaaac gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt
     7681 aaaaaaatgc atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg
     7741 aatgacatat ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac
     7801 ctcaatgaaa gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca
     7861 atggaccaaa tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa
     7921 acggaagaac gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa
     7981 gaaaaagtca gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag
     8041 atcaaagtga tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct
     8101 gttgaagccc gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg
     8161 atgaaaaaaa ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa
     8221 gaaatcagtg atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat
     8281 cttgctgaag atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa
     8341 aaaacagaag ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg
     8401 ttatatgata tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca
     8461 acgattgcag agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg
     8521 gatcacacat ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc
     8581 caaaccgaag tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa
     8641 cacgaaattc cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa
     8701 gatatcgaaa agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa
     8761 aaagaagcgc aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt
     8821 gtagaaaaac aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct
     8881 actgaccgta ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag
     8941 gtcaatcgtt tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat
     9001 gatttagtag atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt
     9061 catacacacg aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa
     9121 tatcactatc aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt
     9181 gctttcaaac gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa
     9241 gttagtaaac cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat
     9301 ttgaaaattg aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta
     9361 taatagaaaa gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa
     9421 caaccccaat ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg
     9481 ggaatccatc aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa
     9541 gaaagcagat cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg
     9601 gaacagaagg agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga
     9661 atcatatgat catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa
     9721 aagaattagg aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg
     9781 acgaattgaa agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata
     9841 atgaagtagg atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta
     9901 aaattcattt ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt
     9961 tgacagaaag agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa
    10021 ctggtttcat ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc
    10081 aagaaagaaa tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag
    10141 ctttacgttt atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag
    10201 tagctctaat caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg
    10261 actatgcacc gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc
    10321 atgccttaga agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac
    10381 atatggcaag cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg
    10441 ttcgaatcag cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt
    10501 tcaagcagct ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat
    10561 acagtgaaat tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct
    10621 ttatcatgca actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa
    10681 tccatgctga tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta
    10741 ttccaaaact agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa
    10801 aagatgtcga agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga
    10861 aagaaacatt taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg
    10921 aattgaatca aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa
    10981 tgcgtcaacc agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt
    11041 atgaaacgat caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta
    11101 tgctttctgg ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg
    11161 aaattgaagc tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag
    11221 caaaagatct aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc
    11281 catttaccga gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa
    11341 ttactcgtcg aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag
    11401 tcattttgaa tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg
    11461 ctatcaatga agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg
    11521 aaatcattga agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag
    11581 attgctgtac gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc
    11641 atcaatatga agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga
    11701 agatggaaga gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc
    11761 tataagaggt tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat
    11821 agctccatgt attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt
    11881 tatcaagaag ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt
    11941 agagatcaag ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag
    12001 gttgtgacag aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca
    12061 aatttttgtg aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca
    12121 gaggttgcga aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc
    12181 tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta
    12241 tttgtctcac acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta
    12301 cactaaatat gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact
    12361 gccaaaagca actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga
    12421 ctcaggtata agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc
    12481 aacgatccgc agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga
    12541 tgtccctttt cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat
    12601 cgcattagta ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa
    12661 tgaaaatcta aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat
    12721 tgatggagaa atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt
    12781 gacagtagca atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt
    12841 caaaggaaat gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta
    12901 tgtcaatgtt caatacattg atttgacaac cgagttgcag acgcttattt actttgatga
    12961 gaattactca gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac
    13021 tcgtcgtaat tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact
    13081 tgttgactaa aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac
    13141 tggcacattc attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg
    13201 aaggtagctt gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa
    13261 ctcacttgag aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata
    13321 cgtcctagaa tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg
    13381 acggaagaaa ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat
    13441 cagcattggt tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat
    13501 tcaatcgtaa tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat
    13561 acgaccttgc aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg
    13621 ctgcctggta tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga
    13681 gagcaaggga tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa
    13741 tggaaagaag agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta
    13801 gattacagta gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt
    13861 tttgttacct tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat
    13921 cctcaagcac gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc
    13981 ttttatcata tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc
    14041 cgtccagaaa cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat
    14101 caagcgctca tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt
    14161 gcagatgaat atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat
    14221 gatccaaacg actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat
    14281 gatgatggta cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct
    14341 cgtaaagcaa tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg
    14401 atccacagtg tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact
    14461 caatggtttg taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat
    14521 caagcagtgg aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat
    14581 gtccatgatt gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat
    14641 cacaaagaaa caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg
    14701 gtacaagatc ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca
    14761 atgggctggc cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta
    14821 gtaaccggtt atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa
    14881 tttactggcg aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa
    14941 gggcggaaga tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag
    15001 tatggagcag atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc
    15061 cgcttcagct atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct
    15121 tcacgtttcg ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga
    15181 gaaaaatcag tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta
    15241 actgagctat ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc
    15301 tgggatgatt tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat
    15361 gctgtttcaa aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt
    15421 ttattgcatc caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag
    15481 ggaaattctc tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca
    15541 gcttctaaag ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca
    15601 gaagtgaata caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa
    15661 atcgatcaat ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag
    15721 ttgacgatat caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt
    15781 gcagaaatct tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta
    15841 gaaaaagaac tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat
    15901 gaacgttttg tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa
    15961 gattatttag agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa
    16021 taagaataga aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa
    16081 catcgttaca ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac
    16141 ataaattttg tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt
    16201 ggatcatgta tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt
    16261 tctctttgtc atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg
    16321 ggggaattgt tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt
    16381 ggttcacgtc ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag
    16441 agaaaagtaa aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat
    16501 ttgagaagcc tattagaaga ggctgggctg acagtaggta catttacttc accttatatc
    16561 gaaacgttca atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc
    16621 cagcttgtaa aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct
    16681 ggtattactg aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa
    16741 gtggacgtag cgattatcga agttggctta ggcggcttat tggattctac caatgtagct
    16801 gtaccaatgc tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat
    16861 acaattgaag aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta
    16921 acaggaaata ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat
    16981 gcaccgcttt atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg
    17041 tggggagaag tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg
    17101 cttttagggc aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac
    17161 tgtcaattgg aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct
    17221 cgatggccag gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc
    17281 cataatacgc atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat
    17341 caaatcaaca ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga
    17401 ttgcagcaag taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc
    17461 gaattaagtg aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa
    17521 ttcggattag cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt
    17581 tcactttatt tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa
    17641 gtataatgca gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta
    17701 cgaagccaca caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt
    17761 aaaattctta ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga
    17821 atttggcaaa acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg
    17881 tttttcgcaa ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga
    17941 agaacatcag attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt
    18001 attgaagaag aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca
    18061 acgagcaaaa ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa
    18121 gaaagaaaca ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga
    18181 gatcccggta gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac
    18241 agctggggtt gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc
    18301 ttttcgtatc ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat
    18361 caaagaagta cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc
    18421 tttatcggat caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt
    18481 gatgtcgatt gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc
    18541 tactttgcat gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa
    18601 agccttgatc gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt
    18661 tcgtaccagc tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga
    18721 acatttggtt tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt
    18781 catcggttcg ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg
    18841 ttattgtgca gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc
    18901 gcagcaggat atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac
    18961 tgtactggac cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat
    19021 gatggaagag aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt
    19081 gtaattttgt ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa
    19141 tgttacaatt taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca
    19201 gtaaaatggt tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac
    19261 gtattcgttc acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa
    19321 gcagtgactt tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa
    19381 gcttaatttt atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag
    19441 cgagtaacaa aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta
    19501 tgaaaaagta aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa
    19561 caaacaaaaa tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc
    19621 tgatttcata acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg
    19681 gatgtctttc tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca
    19741 atttatattc tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc
    19801 gctgagcttc attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa
    19861 tatctggacc agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa
    19921 gaccttcttc tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc
    19981 cgctaccatc gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc
    20041 tagttaacgc taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta
    20101 catcttgtgc tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa
    20161 aatattttgg cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg
    20221 aagtatggat gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat
    20281 tgatcaaatg attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg
    20341 tatcgtctgg ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg
    20401 ccatcgcttc tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat
    20461 tgatttcgtg tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac
    20521 cgttgacgat ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca
    20581 cagcggcatc tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga
    20641 tacgtccttc gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt
    20701 tagtaaatgt tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt
    20761 ccccgatacg agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt
    20821 tgaagtctcc agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat
    20881 ctgggatact atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg
    20941 tatcttcatt gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt
    21001 ttgtgatcaa cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca
    21061 agtagggtat tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt
    21121 tgtgtaagtt ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat
    21181 cgtcttccgg gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc
    21241 catacgtatt tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat
    21301 ttttgatgga aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg
    21361 tactctagat gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct
    21421 tccacacgtt ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct
    21481 tcccgtttag ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg
    21541 tctcctttat ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac
    21601 gaggggacaa gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa
    21661 gagacaagcc tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata
    21721 aaaaatatac aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca
    21781 actagattga aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta
    21841 tgagagaagt gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa
    21901 caaaagatta aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa
    21961 gaaattcttg tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact
    22021 tcctacgatt ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt
    22081 gaagcaacaa gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt
    22141 gttaggttat tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt
    22201 gttcgaaaag atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt
    22261 gatcactcgt acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg
    22321 tatcggtttt atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag
    22381 tgtgacactc agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc
    22441 gattgctaag gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa
    22501 cagtatcttg cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa
    22561 gtttgaagaa aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt
    22621 attccgtttg atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt
    22681 attgatcatc tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt
    22741 gattgcgttt atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt
    22801 gttcatgatg tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc
    22861 caaaccttcg attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat
    22921 cgaatttcaa aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa
    22981 tgtcagtttt acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg
    23041 aaaatctacg ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt
    23101 gatcgatgga gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt
    23161 tattccgcaa aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa
    23221 agaagatgct acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt
    23281 catttctcga aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc
    23341 aggtggacaa aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta
    23401 tatttttgat gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg
    23461 tctgaaaaaa gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat
    23521 catgcatgca gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca
    23581 tcgggagttg ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa
    23641 ggaggaatta gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga
    23701 ccgtatcgtg agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac
    23761 gtggctttgc catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat
    23821 ggcgaatcaa tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga
    23881 atagggtatt gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt
    23941 gccatgcatg atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat
    24001 ttcgataaaa accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc
    24061 agcggtgctt tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg
    24121 gctgcagcaa tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt
    24181 ccaacatcga tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc
    24241 atgcagaatt ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt
    24301 gtattgaaag tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac
    24361 agcttgaaat actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt
    24421 caattaactg cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt
    24481 actggggcga tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt
    24541 cagccaatgg ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg
    24601 aaacgtgtat tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca
    24661 ctgccagaaa cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg
    24721 aagaaaccat taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg
    24781 gtcggtccga ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac
    24841 gtagacaaag gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat
    24901 gtgcgttcgc tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca
    24961 gataatatcc gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa
    25021 acagcaaacg tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat
    25081 tctgagggag acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg
    25141 gtctccgatc ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta
    25201 gaagcgttga ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc
    25261 gctcaccgtt tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag
    25321 atcattgaaa aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg
    25381 tataacagcc aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa
    25441 cttatttttt gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga
    25501 cacggagatg gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg
    25561 tttaacgcac gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt
    25621 tcaaatcgaa cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat
    25681 agaagaaaat atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa
    25741 tagacatctg gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta
    25801 tgataccttt attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg
    25861 gaacggttta tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg
    25921 acgatctggt atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga
    25981 acgaatatcc aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg
    26041 gagaatgtga cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg
    26101 ctgatcggga tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt
    26161 ggtgctggat cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt
    26221 cgcccagcta aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac
    26281 atccagcatt atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga
    26341 taggcagtag catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt
    26401 atttggttta gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag
    26461 aactgttttc catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat
    26521 agttgtcacg atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta
    26581 aaattttgtt taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag
    26641 ttgcttgccc ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag
    26701 caaatcgttt accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac
    26761 ccataacaat tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac
    26821 ctgctgtaac tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt
    26881 gagcgtctaa gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc
    26941 cgcttaacat gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac
    27001 ctcgtgaaga ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt
    27061 tcgtttagaa atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt
    27121 tggtcaaaaa aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg
    27181 tatgataagc agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc
    27241 cttatcggag tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt
    27301 cacttggaaa ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag
    27361 acgctgaatc aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg
    27421 gaagaatatg tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg
    27481 tatctccaat cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag
    27541 ctatttaaga atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt
    27601 ggtggatatg cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact
    27661 gaaatgatca atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc
    27721 ttctcgaaag atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg
    27781 tggtaatgct ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg
    27841 ttacaaatct atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca
    27901 tttgcagaag ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa
    27961 gatggttatt tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg
    28021 ctgattcgag aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc
    28081 aaagaatttc cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa
    28141 aaaaattacc gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt
    28201 atcgaagaaa aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat
    28261 tgcagctttc atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg
    28321 gacttattga tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata
    28381 gaaggagtac atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt
    28441 ccaaaagcaa ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa
    28501 ctcaaaatag atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca
    28561 acgaaaagta ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt
    28621 aaaacagcgc taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat
    28681 tcattgcaaa tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg
    28741 aataatgttc cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct
    28801 gattttcagc attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct
    28861 atcatagctg gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga
    28921 gcgacagaag aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt
    28981 ggttcccaag cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc
    29041 cactggaaca atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat
    29101 agtatcacgg cacctaaaga agagccaaag caattgtatg attacctcat gattggatta
    29161 gatacgaagc gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca
    29221 gaaggtttag tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa
    29281 ggtattggat ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca
    29341 acagaacaag tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt
    29401 aaccgtttgg atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt
    29461 gagcaaacaa tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt
    29521 aggggtagaa accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca
    29581 gctgacgaat accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt
    29641 ggatcggcaa acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc
    29701 gcatgaagcg gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat
    29761 taccgatgct gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc
    29821 gatgcgtgct cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct
    29881 gctgcctcgt ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac
    29941 aagagggcca ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt
    30001 tgctgttaag aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag
    30061 aaaaaatagc gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac
    30121 tatcttgaat ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt
    30181 agatccattg actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac
    30241 cgtcgggttc atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga
    30301 agaaagtcaa aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat
    30361 tttacaggag cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat
    30421 actgactgtt tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt
    30481 cccaaatgta ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat
    30541 caaacgtcag ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg
    30601 acagaaatta agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga
    30661 aacaaacgaa tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa
    30721 ccaataaaat tttatttttt taa
//
LOCUS       DAZUJO010000027        29976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000027 DAZUJO010000000
VERSION     DAZUJO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.027"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..965)
                     /locus_tag="ACG8OL_001310"
     CDS             complement(21..965)
                     /locus_tag="ACG8OL_001310"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4817309.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1261..2205)
                     /locus_tag="ACG8OL_001311"
     CDS             complement(1261..2205)
                     /locus_tag="ACG8OL_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS4817310.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2426..3640
                     /locus_tag="ACG8OL_001312"
     CDS             2426..3640
                     /locus_tag="ACG8OL_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4817311.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3662..5677
                     /locus_tag="ACG8OL_001313"
     CDS             3662..5677
                     /locus_tag="ACG8OL_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS4817312.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5760..7133)
                     /locus_tag="ACG8OL_001314"
     CDS             complement(5760..7133)
                     /locus_tag="ACG8OL_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4817313.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7159..7443)
                     /locus_tag="ACG8OL_001315"
     CDS             complement(7159..7443)
                     /locus_tag="ACG8OL_001315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4817314.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7445..9460)
                     /locus_tag="ACG8OL_001316"
     CDS             complement(7445..9460)
                     /locus_tag="ACG8OL_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4817315.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9639..9944)
                     /locus_tag="ACG8OL_001317"
     CDS             complement(9639..9944)
                     /locus_tag="ACG8OL_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS4817316.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10033..11064)
                     /locus_tag="ACG8OL_001318"
     CDS             complement(10033..11064)
                     /locus_tag="ACG8OL_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4817317.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11271..12146
                     /locus_tag="ACG8OL_001319"
     CDS             11271..12146
                     /locus_tag="ACG8OL_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4817318.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12298..13140)
                     /locus_tag="ACG8OL_001320"
     CDS             complement(12298..13140)
                     /locus_tag="ACG8OL_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4817319.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13232..15109)
                     /locus_tag="ACG8OL_001321"
     CDS             complement(13232..15109)
                     /locus_tag="ACG8OL_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4817320.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15106..16527)
                     /locus_tag="ACG8OL_001322"
     CDS             complement(15106..16527)
                     /locus_tag="ACG8OL_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4817321.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16530..19919)
                     /locus_tag="ACG8OL_001323"
     CDS             complement(16530..19919)
                     /locus_tag="ACG8OL_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4817322.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20176..21567
                     /locus_tag="ACG8OL_001324"
     CDS             20176..21567
                     /locus_tag="ACG8OL_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817323.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21640..22287)
                     /locus_tag="ACG8OL_001325"
     CDS             complement(21640..22287)
                     /locus_tag="ACG8OL_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4817324.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22295..22981)
                     /locus_tag="ACG8OL_001326"
     CDS             complement(22295..22981)
                     /locus_tag="ACG8OL_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817325.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22987..24060)
                     /locus_tag="ACG8OL_001327"
     CDS             complement(22987..24060)
                     /locus_tag="ACG8OL_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817326.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24282..24956
                     /locus_tag="ACG8OL_001328"
     CDS             24282..24956
                     /locus_tag="ACG8OL_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4817327.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24961..26301
                     /locus_tag="ACG8OL_001329"
     CDS             24961..26301
                     /locus_tag="ACG8OL_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4817328.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26450..27436)
                     /locus_tag="ACG8OL_001330"
     CDS             complement(26450..27436)
                     /locus_tag="ACG8OL_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS4817329.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28160..29191
                     /locus_tag="ACG8OL_001331"
     CDS             28160..29191
                     /locus_tag="ACG8OL_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4817330.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29300..29725
                     /locus_tag="ACG8OL_001332"
                     /pseudo
     CDS             29300..29725
                     /locus_tag="ACG8OL_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            29838..>29976
                     /locus_tag="ACG8OL_001333"
     CDS             29838..>29976
                     /locus_tag="ACG8OL_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS4817331.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT         8904 a         5623 c         5391 g        10058 t
ORIGIN      
        1 tagttcatat tcattgtttc ttataataca gtgttcaata cttctcttaa agcacttgtt
       61 gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac ttcacgaaca
      121 ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc gtattcacca
      181 gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc ttttacgata
      241 cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat gatctcataa
      301 gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat acgatggtct
      361 ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca tgcagcaact
      421 tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc aacttttaat
      481 ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt accgataaca
      541 cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc tactgggttt
      601 gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac gatttggcgc
      661 atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt ttggttgata
      721 ccagctgtga taacaacgat atcagcatct ttacattctt catagccacc tgaccataca
      781 gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc cccttctgct
      841 ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt gatcattgca
      901 taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg acttgttttt
      961 ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa gtatagtctt
     1021 gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt ttgatggaaa
     1081 cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata
     1141 atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca acgatataat
     1201 aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa gattttcttc
     1261 ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa
     1321 taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt
     1381 ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga
     1441 agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc caaaatgagc
     1501 tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag
     1561 catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt
     1621 caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt
     1681 atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc
     1741 gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga
     1801 agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc
     1861 ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgagtagc
     1921 ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc cattaataat
     1981 ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg tatgctcggg
     2041 aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa atagatcccc
     2101 tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat tgatattcat
     2161 tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa atgtctaatt
     2221 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
     2281 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
     2341 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
     2401 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
     2461 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
     2521 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
     2581 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
     2641 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
     2701 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
     2761 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
     2821 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
     2881 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
     2941 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
     3001 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
     3061 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
     3121 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
     3181 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
     3241 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
     3301 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
     3361 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
     3421 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
     3481 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
     3541 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
     3601 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
     3661 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
     3721 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
     3781 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
     3841 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
     3901 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
     3961 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
     4021 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
     4081 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
     4141 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
     4201 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
     4261 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
     4321 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
     4381 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
     4441 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
     4501 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
     4561 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
     4621 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
     4681 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
     4741 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
     4801 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
     4861 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
     4921 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
     4981 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
     5041 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
     5101 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
     5161 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
     5221 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
     5281 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
     5341 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
     5401 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
     5461 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
     5521 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
     5581 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
     5641 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
     5701 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
     5761 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
     5821 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
     5881 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
     5941 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
     6001 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
     6061 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
     6121 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
     6181 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
     6241 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
     6301 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
     6361 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
     6421 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
     6481 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
     6541 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
     6601 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
     6661 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
     6721 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
     6781 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
     6841 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
     6901 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
     6961 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
     7021 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
     7081 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
     7141 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
     7201 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
     7261 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
     7321 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
     7381 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
     7441 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
     7501 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
     7561 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
     7621 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
     7681 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
     7741 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
     7801 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
     7861 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
     7921 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
     7981 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
     8041 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
     8101 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
     8161 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
     8221 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
     8281 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
     8341 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
     8401 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
     8461 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
     8521 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
     8581 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
     8641 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
     8701 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
     8761 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
     8821 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
     8881 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
     8941 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
     9001 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
     9061 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
     9121 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
     9181 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
     9241 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
     9301 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
     9361 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
     9421 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
     9481 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
     9541 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
     9601 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
     9661 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
     9721 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
     9781 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
     9841 tctgtttcac cagaattagt tcctgctaaa gccacttctt cttctgtttt aggggccatt
     9901 gaacctctta atcgtttgtg tttttcaccg tcataatttg tcatgttggt tcctcctagt
     9961 agttctatac tttttcatcg tacaataaga cagtttatgc gtcaaaaaac aaacccgatt
    10021 cttacaaata tgtcatattt ttccttctga ttcttgaatg cttttgattc cttcttccaa
    10081 aattttcatt tgtaagattg tttcttcatc tttgatgact ttttctgcgc cattaactaa
    10141 tgtctcgtaa atcccttcgt agacacggct gtaatcacca acctcggaaa ttactttttc
    10201 ttcatgataa ctcccttgtt catctatata ggtcaataca ccataatgtt cgggcaaatc
    10261 gataccaaag tcttcatgat caggcatgta aaacattttc agatgttctt cttgtcgatc
    10321 ttttgtttgt ttcacgaaca tccctttttt tccgtaaacg acgaaacttg gacgttcttt
    10381 gatgcggaaa taactggatt ttatcgaaat tttcatattt ccatagaaaa aatccagatc
    10441 gaagtaatca ttcattcgtc ctttccctaa aagttgacgt acatcatagc tgatatcatc
    10501 tggttcgccg aaataagaaa gaacttgatc tactgtatga caagcatgtc catataaata
    10561 gctgttcgca gtagaaaatt catgcgtatt ttctggtact tccggtctga aataatcata
    10621 atgcatttct acttctaaaa tatcccctaa ttttccgctt tcgattactt tttggactgt
    10681 caagaagtca gaatcgaacc gtctgttttg gtaacattgg ataaatagac ctttttcttt
    10741 tgctaatgca aataactctt ctgcttcctt tgacgtttct gcaaaaggtt tctctactag
    10801 tacattctta ccatgttcga gtgccatttt tccatattca taatgaaatt gactaggtgt
    10861 agtgatgaca actaattgga tctcaggatc atcccaaaga tcagacagct gatcagtata
    10921 ttcgatgtct tcgaatgttt tccacactgg ttcagatggg gaaaaaattt ttttgatatt
    10981 gattttgttc gtaagttttc ttgaaaaagg caaatgatag cgatttgtac tctttccatt
    11041 gccaagataa gcgattgtaa gcatgagtgg tgcctccttt tattgatgtt ttcagtatat
    11101 cctagaaaag cgcagaaagt atttataata gctattctgg taaaaaagtt cagaaaaaca
    11161 cttgttatca ttcggtttgg tgaaattaat gttcaaagta aggataaaaa tcaaaaagat
    11221 agtagcggaa ccaagtgttt atcagtaaga tagaatttgg aggtcagatc atgcttattg
    11281 aagaaaaact aacaaaacaa gagttgttta ccacgacaga gaaacggatt gtcgattaca
    11341 tccgtaggaa tatcgaagct gcagtctaca tgacgattga agaattagca aaagctacgt
    11401 atacttccca ttcagctatc attcgtttat gcaaaaaaat gggattcagc ggatttaaag
    11461 aattccgttt cgagctgggg cgggaagttc atcaattgat tgctcagttc aatcaaacga
    11521 ttgatcctaa ttttcctttt tcaagtgaag atagcactca gcaaattgca aagaaaatgg
    11581 cggatttatc gatccagtca ataaaaaaag cacaattgca gattgaacat gaagatttag
    11641 atcaaatcgg taaagtattg aacaaggcac agcgaatctt tctttttgca aaaggtgatt
    11701 ctcaaatcac ggcaaggaaa ttccagaata aaatggtcaa gttgaataag tttttgatca
    11761 tggctgaaga atacagtgat tcttcttgga atgcagccaa tttgactagc gaggattgcg
    11821 ccatttttat tagctacagc ggtcgcatcc atcattatga acgaataatg acctatttga
    11881 agcatgtaga ggcccctacc ttgctgatta ccggaaatca gcattcagaa atggccaaac
    11941 aggctgatat gtgtcttgtt atatcgcagg aggaatatga ttttgctaaa gtagcgactt
    12001 tctcctctca aattgccttc gattacgtat taaatacgtt attctcagtg atatattctc
    12061 aaaattttga agagaatatc cgcaacttga aaaacaaaca agaattgatg caaaaaggcc
    12121 ttcttaaaga atatcggaaa acttaagaaa agatcctcat ttagtaataa gaaagatagt
    12181 gtttgatagg ctctctttga tatcatcata aaaacaggct gaagttttct ttttcaaacc
    12241 tcagcctgtt tgtttacgga acgattcgaa tagatatatt atagaaatga ggaatagcta
    12301 acttctgttt cttattttga acgattgacg aaaaagatat ataagaagga ggaaaatcag
    12361 tatacatgca gctaacaatc catacaggaa aaatcgtttg ttctcggata ctttctgtac
    12421 ttctttttga acttcttttg catgataagg aacccggtgc cctctgacaa gtaagcgatg
    12481 actgttgacc atataaggtg tacaagttag gagtgtcacg aaatcctgcc ctttttcgat
    12541 tctcagatct tttgtatcag tcggttctac cgtcttgatc tgatcgactt gataagcgag
    12601 tgtccgtcca ttgatttcga tatagaaaga atcacctaaa gtcagtttcg gtaagtctgt
    12661 gaacaacttt gcttgaggca gtcctcgatg gctggaaata acggcatgtg tattttctcc
    12721 ccccgtagga taagacgttc cttccaataa tgaggcacct ttttctagca gcacagtatt
    12781 cgtttcatca aaaattggca aatggacatt gatctcaggg atcgtcaaaa taccgatcgt
    12841 atgtttctca aaataggttc tatccttttt cgcaggttct tttttcttag taaagggatc
    12901 tgcacctgga tttccacctt cttttgctaa ttgctggttt ttctttgtca tgttttcttg
    12961 gattttagcc atgacttctt cattttcttt gaccgcttgt tgctgataat gagagatgat
    13021 ctgctgatcc agataattat tcagcgcgtc gctaacaaat ggataagcta gtatccctat
    13081 tcctgagaga agcaagaaaa gcatcagcag atctagaagt ttccttttct ttggtttcat
    13141 ttgtgttctc cttgtgatga ctgtttgata ataaaagaaa aaggagcatt tgcgtaatcc
    13201 gctgctcctt tttccaattc ttgcaaaaag actaaatctg actgtgcttg cgtctagcaa
    13261 agtacagtcc agcaatcagt agaaggacta ctcctgcacc gatatacaca tagattccct
    13321 taccgcctgt tgaaggaagt gtacctttgt gtttatttgg tattttttca ggagaaatca
    13381 actgtcctgc tgttacataa gattgttcat cgatagtaaa tgctacacgg tttgttaatg
    13441 gaacatattt ttctggcgct ttcgtttctt ccagatagta cgtgccatat tttagacctg
    13501 tcacatcgat taaaccgtta cttgtggttg taaaaaccgt tgctgattct ttcgccgata
    13561 cccagctgac ggcttttgtg gatgggtcga tcgataaata tttcgctgtg tcactatctt
    13621 gatcacgaac gacgaattct gctccagcaa gtgtttggtc tgatgtaacg tcaccatcta
    13681 ctttaacaaa tcgtttgccc ccagtaacga catcgactga cggtggtgtt tgatcatttg
    13741 tatgcccgtt atcgacattt gcttggttag aaaatccttt ggttggatct gctttttcgt
    13801 tcaaatgcat atagtaaacg aatttcaatg taccgccagg agttaatgaa ggaatataat
    13861 tcggatcaac tgaaaccgtg aatccgtctg tttgctcagt gacagaataa tttactgggt
    13921 cgatttcttt atttccatca tataaggcat aagcatacgt tcctgaagaa tcattatcaa
    13981 atgttaaagc agcgtcatga gtatcgataa gtttgaatgt tgtgtacttg ttttgcgttc
    14041 cttctttatc agcgatacct aatgggatat tgacagaaat ctcatattga attttttcac
    14101 cgattgcgac atcttttcca ttcaattgag gtgttgtttt atcaacttta gacgtatcat
    14161 ttttgatggt cttttctact ggtgtttggc tatttgccag gatctcaaaa ggcgtgattg
    14221 tttgcttttc aatcatttcc gcattatcag gagcttttac ttcttctaaa ttatattttc
    14281 caacttctaa atgattaacg atcaactggc ctttttcaat agatgcctcg gcaaagtcat
    14341 tgttgccgat gtcataagaa tgaccagtaa tgaaatgttt ggctttggtt tgatcagttg
    14401 tccaagtgta caatccatct gtgacacttt ggatgtattt tttgacgctt ggtgttcctt
    14461 cttctttaga aataataaat tctgctccat ttagtgcttc gttttcggca gtaccgattt
    14521 ttgtaacttt caacgttcca tcattaccga ctgtattttt agggtagaga tggatagtat
    14581 ctagttcttc tgtcccgtat ttataagagc catctgcttg tttgatcatc tcatatacag
    14641 ggaaagctaa aaccatgttt gcggcagctg acactccgtc ttttggttct tctttgatcg
    14701 tgtagactgc atctttccca ttttgttttt taggtaaaga taaagtgaca tttccatcag
    14761 catctgtcgt tcctgaagca actggtgtac caggagtcaa agactggact gcttgttttg
    14821 ctgcatccac ggacgctcct ttatctcgtt gcgcataaaa ttcttgagtg acgttataaa
    14881 ctgaaaatga aatatcggct aatccttggt attgatcgaa ttcgctcatt tctttcccgc
    14941 tgttttggat taaaggatcg ggtaaatcag tcattttctt tttatgaagg atgacttgag
    15001 ctgctgtctc ctctgcaaac actttttttg cgccgaagct gtttgtgagt aatggtaaaa
    15061 taaggaaaag gactcctaac ataacgttta tttttttatg atttttcatt ttttcactcc
    15121 tgttcttttt ttgataaaca accagattgc aaagcttgcg aataccatac caacaacgcc
    15181 taaaagtgta tttttcgttt cgtttgtttt tggaagaaat ggacggctat tttctttttt
    15241 attgactact ttcaatatac tgtccgcctc tttatcctca gaaagaaaag ttgaaatggt
    15301 aaacggaatt tctgtttcac ttaaaatata accagaaggt gcttcgatct cttttaatcg
    15361 ataacttcct gttttcaacc cggaaattga aaaagcgcca tttttatcgg aaatcaggtg
    15421 aagcgcatct gattcgttct ttgtccactt gtatccgttg gctgtttgct ggaggtaatt
    15481 tcctgctttg tttttaataa ggaatactgc gccagctaat cgtttatctg gtttatttgt
    15541 ctcgattttg acaaattgct tactccctgt atatacagag actttctttg tactgacacc
    15601 aaaaccaaaa tccagtgttc cgttgttgtt gatttccttg ttcgcctgtg ctgtactgct
    15661 tagctgcatc tgataagaaa tggtcagttt ttttccagca tatttttcca acgaagggat
    15721 cgaaaataaa acatcaaatc cgtgaggttt cttgtgtatg acaaagcctt ctgtcagctt
    15781 ttctccattc gatgaaatcg ttaaactatt aggtaaaaac gttaatgctt catccgcact
    15841 atctgacaat tcgaactttt gatagtcaag gatatttaca ggaattgtcg tatgcaagga
    15901 ataagtgatt ttttcaccaa tcgtaaaatc atttctaggc tcttctaata ccttctcaaa
    15961 aggtggatca gggtagtcgt tttcttcatt tttaggatac aaatggatag ttgaaagttt
    16021 ttgattgttt tgtccatgta caggaagaac aactaccata ttctctgcct tttctttgac
    16081 gacttctggt actttggatt caatgaaaag atacgcttta tctctttttt cttgatcttt
    16141 agcggccaga gaaaaagaag cgataccatc ttcgttgtta agagttgtag ttgttgcttc
    16201 tgcggtaaac ataccggaag acgcaccgtt ttttgcgatc tctgcttgtg cttcttctac
    16261 cgttttgccc ttttcccgta gatggtaaaa agaatctgtg acatcataaa cttggaatgt
    16321 gacaccattt aatcctcgat acgtttgtaa taaagctttt tcttgtccgt catttggatg
    16381 atttttcggc agttgcccgt ttgggaaaag caatttgtgt aaagtaatct gaaccatttc
    16441 tgtttctgtt tctgttgcta cctgagtaaa agctggttta aatagtaaca agaagagaca
    16501 catactaagc caaccaagtt ttttcatatt tagctgcccc ctctttcttt tttcaaaaaa
    16561 agataccaaa tagaaaacgc acaaccaatc atccctacta gatagattcc taaacggcca
    16621 attcctcccg tttcaggtaa tggtactttt gcctgattcg tgatgtctaa agaaatctgg
    16681 ttgtttttgg ctcctggtga cagaacagat tcatgatctt gtccatccac ttgaattgac
    16741 ccatcttcgt gtataactat agtaactggc tcttttagac cttggtatcc ttctggtgta
    16801 aaagtttcgg tcagcgtata agttccaggt tttaaatttt cgaatagaaa ggtatctgtt
    16861 tcttgtccat cttttggcga ttctaagtcc atttctggtc cctgcagtct gaattttgct
    16921 ccttttaatg tctgtccgtt gtcttctttt ttgtgtaccg ttagatcaaa cgcttttagt
    16981 cgattttgat gagtgagcgt ccatacatta tttgtcatct cggtattttt tcccgtgtaa
    17041 atgatttttc catatttatc gatttggaat tcataatttc ccggcgaagt atcatatccg
    17101 gcaggaccag ctgtttcaac gactcgatat ttaccaggtg tgagtgaatc aaaactggca
    17161 agaccttttt cattcgttgt ttggctgtca attggctgat aagtaccatt tgcttctttt
    17221 ttctgtaggg aaaatgctgc tcctttaaga tttatctctt tgtccgtccc ttgcatcgca
    17281 tacttacgta ctgctacagg aacttcaaca aaaggatttt caatagtcaa ctggatcgta
    17341 tcatcggaag tagtgactgt ttttccatca atggttaccg taccatcaga agcgatcttg
    17401 atttcccaag tagtcttttt gcttaatcca tgcccttctg gagcttttgt ttctgtcagc
    17461 gtataggtca tttctttttg caatttgaca ttttctggaa gagaataggt tccgtcgcca
    17521 tgatctgtta gtaaagtatc aatagaatct cctgttaatt tgaaaacagc gccaataaga
    17581 tccttttcac ctgtagagga atttttcgtt attttcaagt ttaacggtgt gaattgttta
    17641 gtatttttcc atgtcattgc atctatttgt tgagaatcgt atcctggtac aggtaattct
    17701 ttgattgttt ggtaactgaa tgcttgacct tgattattgt attgaggtag tgataatatc
    17761 tcttgataac tttctccgct atttgcagat aatttgtcaa tgtctgcacg ttcccacgta
    17821 tttgttgtat ctttagctgg tttaatgatt cgaatatatc cgtttttcca agctgcagca
    17881 tttgtcgtat gttcccgttg aatctcaaaa gtaacttgat ctggacgatc agctagattg
    17941 ttgtcaaatt cttcccataa ctttttgatg tgaagactga ctccgggagc tttagcagat
    18001 ggtataccga attcagctaa ttcattggta tcaatgtttg gctggaaagt tgtcctgccg
    18061 ttcatttgat accagaaatt tggatggaag tcctcatttt ctgtttggat tctcacttgg
    18121 taatggattt ggatttcttg gtcttttcct aaatagatct gattgctctt gatggtattt
    18181 ccttctatgg aaatagatgg agtgaccgtt gtaggacttg tccccacact cttgactgaa
    18241 agtgtaccag gctgataaat gaatggttct gcgattggat catttatttg tccattgctt
    18301 acagttgctg agatctgtac agcttttttg gctaggtatt cagagatatc tgttgcatga
    18361 tcagctgatt catagtaaag atcgcctttt tcatctgatg aaaccatttg tcgcatacga
    18421 gactctactt ctgcttttga gagaccagct gccggatcgc tttgaagttg gatgccaaga
    18481 ccatgtattt cgattcctcg ttctttgagt gccatcgctt ctccgatggt tgcgataaac
    18541 gtactgtcga ttcttctgga tagattgttt tggtcaggtg catcatagat tcttgagatt
    18601 agagaagtat ttcccggccg atcttgcgta ttagaaaact gagttccgta ataattgctg
    18661 cttgattgtg cgtgtacccg ctgtacttta taggaaaatg ttggtacacc atccgtcagc
    18721 aaaacgatca cttttttatg tccatttgga acggatagca tgcttcctgc atctcttagt
    18781 gctttttgtg taaaagtacc accatttgtc cgtgaaggtg taatggattt tacttgattt
    18841 ttcactgaat caaatgaacc catctgtact gcaccgttac tgtagctata tccttcgctt
    18901 gagtagccga catatcccat attgattttg tctgtgatac cgctatctgc taaagtatcg
    18961 acaaaacgat cgacaccaat ctttacttca ccgatccgat tattgtcgtt catacttcct
    19021 gaccagtcta cgaccaatac gagatcaaga ggcgtgatat ctttttgtac atttccacga
    19081 gcattcaaat aaacatcaaa caatccgggt gtacttgttt cctttgcata ctttcgtatc
    19141 gcatagtctg cttgattacc ggttcctcca tattcgatat acgaatgggt ccgatcatta
    19201 ggatctccat tccaagaatt tataccatcc cattgatttg ttccgttttt attgccttga
    19261 tgatcaagaa catttgtatt tcctgtaggt tgccaactgg ctttaggata agtgccctga
    19321 tcatctgttg tatattctgt aggaatcgtt tgattagatg gcacactcac agctggcaag
    19381 gataatgtag gcaatgtaaa tgggctgaca gaattttcgt tttcttttga tgattcttct
    19441 tctatttcgt ctggctttgg tactgacaca tctgtatctt tcttattttc tgtctcatca
    19501 ataatgtcat attctttatt atcgacgatc agcttgtttg cctggctgtg gtaaagtgct
    19561 ggatcgattg gaaaactcag atcgatagtt tgtttagtgt ttgatgagat ttccaacgta
    19621 attttcccgc tatttacctg ataagcatag ccatctcctt cttttctttc cacatcactt
    19681 aagtaatttt cgactagacc aatttctttt tctatctttt cagcactggt gttattcgca
    19741 tttagcttga tgttcagtgc tttttttcca gactgatccg aaatgacttc agctgaaatt
    19801 gtttctaaaa ctgttggatc atctgtagtg tctgctattg cagataatgg cacaaagttt
    19861 gataaactca atattatcag catgaaaata actttcagtt tcttccctgt ggtcgtcatt
    19921 tttcccctcc tttttctttt tttctgtttt gacgtgaata gtgaaaatta ttaaaataaa
    19981 caggcgttac ataattttat ttattttaat atgataatta ttatacgaag ggcgctaaag
    20041 ggaatcacac caattttcat accaattggt ttcatttatg aagttgattc cttcgattaa
    20101 tgagcgtttc attgaaagaa aagggttgag ggagaaaaac atatgatcag gttgttggaa
    20161 ttaaactata gaaagatgga aaaaatcatt cgaattctgt ccatttctga caagtggatg
    20221 actaaaaaag aacttgccaa acgaattggt agttcagaat ctacatttgt aaggtatatc
    20281 gaagagatca aaatccgctg gggaacggat gtaacgattc aatcttcaca aaaattgggg
    20341 tatcgtcttg aacatttgaa tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa
    20401 agttctacaa acagtcgttt attaaatgag attattgtca atccgggtaa aacgattgag
    20461 tattattgtg aaaaaatttc catcagccgg tcttctttta cacgtaagtt gaaacaatgc
    20521 aatcaaattt tagaaagcta tttattaaaa gtgatcgttg accaaggttt tcagttaact
    20581 agccttaaaa gtgagctcca gttgcgtatc tttgttacct ttttcttttt aagttactac
    20641 ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag
    20701 aataattgtc agatgaattt gtttcaggaa gaaagcccat atgagcaatc tttctttatt
    20761 atgtattttg ttgtgcatct catgagagaa tcccaaggaa ctccatttga atggaaagct
    20821 ggagactctt atgtacagtt gataaatatc gataaaaaag acttcttaat cttgaaaaat
    20881 ctcttaggaa aattgaagta cagcagctat ataaaagtag tgaactattt tgtcaatacg
    20941 attttttcag aattttcgga gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat
    21001 atgtcctact gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt
    21061 caattattaa cgcaaattta ttttttctcc cgtattttcc catacgatac gactcatttg
    21121 acgatccgtg caacttactt tgcacagcag atgcaacacc gccagatacg tctatatgct
    21181 ggatttcgtg cagaacttca attttattcc actctactga atcagaatct gatttattat
    21241 ttatctagct atatcttttg gatgcttatc ggtaatcctg aaattcacca ttatacttct
    21301 gataaaaaaa tactgattat cagcgactta agtcttcgac atagtcaata tattgaagaa
    21361 tatatcagtg atattcttgc tgtgcataaa attcactctg agacaactgc tattactgaa
    21421 gatcagctgt cgaaatacaa tcttctcgag tatgatctga tcgttacgaa tcaacccatt
    21481 ttgaatgccc atgttcctca tatattgatt gatgatagtg tgtcattcgc aaatgaagaa
    21541 gaactcatcc gcttattcga gttatgaata atcaaaaaac agctatgtat aaaccaccct
    21601 gataaaaatt tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat
    21661 ccaacgagat ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat
    21721 gagatccgtc aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc
    21781 ttccgacgat tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat
    21841 tggagaatca gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa
    21901 caagtataac ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata
    21961 tttttcgatc cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg
    22021 atggactatg tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa
    22081 atatttataa aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac
    22141 ttggctgatg tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt
    22201 tgcgttcgat aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat
    22261 tatacgtgga tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg
    22321 tcaagtaccc atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat
    22381 gagtaaccat aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga
    22441 cttcatatgc atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg
    22501 gctcattata taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt
    22561 taggataact atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg
    22621 ttgaatcttc tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta
    22681 aaaaaggaat caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg
    22741 ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag
    22801 tcggtgtttg caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac
    22861 caatgatgct gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga
    22921 cctttgtatg accagaacca aatgttttgg taactttttt catcgttaat atttttttca
    22981 tttttattaa cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa
    23041 caatccgcct agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca
    23101 aaaaacagca aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag
    23161 agcaagcaga acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat
    23221 gaagaaattt ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt
    23281 catcacgtac atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat
    23341 cgcatttaag gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat
    23401 agttaataac tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat
    23461 tacgatccca ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa
    23521 agcagtccac gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg
    23581 aaagataccg acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat
    23641 tttatagcct gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc
    23701 atgccctttc gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac
    23761 attttctttg ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc
    23821 tccttcaatg tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc
    23881 atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc
    23941 tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat
    24001 accgacgatc aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat
    24061 ttgtttttcc tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa
    24121 ctctaaagtg acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac
    24181 atctttcgat aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt
    24241 tccatgataa actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag
    24301 tagaagacga taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga
    24361 taacacaagt ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc
    24421 ttgcaatctt ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac
    24481 ggaaagaaaa aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata
    24541 agattgaagg atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag
    24601 aactgatcat gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg
    24661 ttctgaagta taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac
    24721 aagttttgcc catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg
    24781 gaatcatttt gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga
    24841 cgattacagt tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat
    24901 tggataaaga aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca
    24961 atgaatggag atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca
    25021 tttgctttta tcttttttct tttgggattc attactttac agatattaaa ccaatctgca
    25081 tacagagaaa cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta
    25141 gagatacaaa gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac
    25201 agaacaaaca tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg
    25261 atcttgtggt ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac
    25321 caaatcaaag aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta
    25381 aatgaagaca gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg
    25441 aatcaagatg gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca
    25501 ttagcgactt ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt
    25561 ggtatcagtt attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga
    25621 caaaaagaat ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa
    25681 aatagtttgg aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc
    25741 atcgctcaag cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca
    25801 atcgctcgga atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa
    25861 tatatcgaac aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt
    25921 attctccata acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt
    25981 ttggttattt tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg
    26041 gattcatctg ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc
    26101 agtgaagaag atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg
    26161 aaagagactg gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat
    26221 catggacaga tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg
    26281 ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc
    26341 acagcccttt tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc
    26401 gaaatttctt taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt
    26461 tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa
    26521 catcttctgt cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac
    26581 gaataactgt atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt
    26641 catcttccaa tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga
    26701 tgacgatttc ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt
    26761 tgtggatgtc gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt
    26821 ttagttgaat tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg
    26881 tacccattgt atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca
    26941 atggatccgt tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag
    27001 tgccagtttc taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca
    27061 ttgtatcttc tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat
    27121 cagaaatatc tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat
    27181 cataggtatc aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac
    27241 catccatccg attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc
    27301 tagatgctgc ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac
    27361 gcaaccgttc aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta
    27421 aatttttctc ttccatgtaa attcctccta gcaaatagat ttatgattgg tatattgtta
    27481 cttataaatt tattctatag gagacgattg ttttctacaa atatttagat tggaatttga
    27541 ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa tgaaagtcat attctaataa
    27601 aatgtcattt gtttctatat gaatttttat gaaaaatttc atataatttc cactcgttgt
    27661 gatggtttaa tttatccaat ccatcaataa gcaaacaact tatataaaaa aggattaatt
    27721 ctcaatataa ttgctcctac catagaataa atcttcatgt aaagtaatga agtaccaact
    27781 atttctaata cttctttaag aattatccca acaatatcgt tagacaaacc attattgaat
    27841 aaatggagag ccaatcgatt ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag
    27901 agcctcctgc cattagaacg aaccaaatcc atttcattgc tcatgaagta accacatagc
    27961 taaaaagagg ataaatcaat taaaagatca aaagaatgac tgcactaata aaataactaa
    28021 aaatgtatct tcttacttca tatcttttag ctagtactta gttaaaaatt tgcttcctaa
    28081 gatcaagaca aactgtctat cttcatttga tgaaatgcgg taaaatatag aaaatgaaca
    28141 ctattggagg gaatttgtaa tggacagcaa aactagaaaa caactgcaag aaaagttaaa
    28201 gcaacgtaca cacttgctcc cttccgtctt tcaacagttt ttagaagaaa atcgttcttc
    28261 cctttcatta ggcagtcgct atgaatacgc aaaagatttt catctgttta ctgattattt
    28321 gagaaaagta ttttctgaag aagtggatga tcagctgatt atccatttag aaaaacaaga
    28381 ttatcagaat ttcttagcta aaatctatcg acatactcgt tcgttccaaa ctacagcgaa
    28441 ccaagaaacg gagcaattgt ttgaaaatag ctattacggg atttcaagga agcaatattc
    28501 attaaatcat tttttacgat ttttgtatca aaaaggactg attgaagaag agatttctct
    28561 tttgggtaca agtttaccgg aaacaaccaa agcagctccg cgatatatcg atgcagaact
    28621 tttcaaagat tttgagcatt tgtttatccc gattgaaggc tttcaaacaa gcagagaagc
    28681 tagctttgaa gaaaagaata gaccgaggaa ccttgcaatt actgccattc ttctgtattg
    28741 cggattaaag atccatgaag tagtagaact taaacgtaaa gatgtatctt tgaccaagca
    28801 gattcttaat ctgaatagaa aagaaaacag actgaataaa gtaccaatac caaaagaagc
    28861 gatgccattt ctggagaact attttaagtt aacagcacaa aatacggacg aaaattcttt
    28921 tgtttttcgt tcctcacatg atcagcaaat ttctcctcgg actattcgcc aaattttgag
    28981 caaaattaca gtatataaaa acgtatcgcc agaactatgc agaaaaacat atcgttatta
    29041 tgcagaacta tatttagatg atgctgacgc ggtaaattat ttatgtggta atcgatacct
    29101 ctcatcccat tcttttcaag atatctggga aaagatggtt gatttttctt atcacgaatt
    29161 agcggcattc gttcgggttt cttccatata aaacaaatgt atgatctttt tttcacaata
    29221 aactatgtta ttatataaag gattatgtta cgattatctt gaatttgtat attacttaac
    29281 aaaagataat ggagtgtgaa tgatggagac cataacgtat tcccctttga acttatttac
    29341 gaattttcaa gaagctgcag ctaacttttc cgagaccccg attatctttg atgagccact
    29401 ttcagctttc ccagaattag gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt
    29461 gaaacgggct tatcaattgg ctcatcttgg agtgaaatcc ggagacaaag ttattattta
    29521 taagagttcc gcttttgaca cataccttct agctgtttct gtgtcttacc ttggtgcagt
    29581 gcctgtcatg acttcttatc atctaccaac aacaacaatg gaagtattta tcgatcgttt
    29641 agaagaccct ttcattttat ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa
    29701 tggaacaaaa tcaaaacaga ttcctaaccc gtggtgctag ttaatttttt cctagaggaa
    29761 aatgtctaaa catgttggta aatcaacaaa aaaacgcata tagtagtctc aaaactccca
    29821 agaaatgagg actacatatg cgttcatcaa aatatactga acattataca gatactttta
    29881 taacattcaa ggaaattatg agaactgttt ctaacatata ccacaattgt gtaccagata
    29941 agattaaaaa ccgaagaaat actgatcaac tgaagc
//
LOCUS       DAZUJO010000028        28053 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000028 DAZUJO010000000
VERSION     DAZUJO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.028"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..542)
                     /locus_tag="ACG8OL_001334"
     CDS             complement(<1..542)
                     /locus_tag="ACG8OL_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4817332.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVL"
     gene            complement(618..764)
                     /locus_tag="ACG8OL_001335"
     CDS             complement(618..764)
                     /locus_tag="ACG8OL_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFS4817333.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(868..1179)
                     /locus_tag="ACG8OL_001336"
     CDS             complement(868..1179)
                     /locus_tag="ACG8OL_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4817334.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1181..1378)
                     /locus_tag="ACG8OL_001337"
     CDS             complement(1181..1378)
                     /locus_tag="ACG8OL_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4817335.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1772..3499)
                     /locus_tag="ACG8OL_001338"
     CDS             complement(1772..3499)
                     /locus_tag="ACG8OL_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS4817336.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3492..4685)
                     /locus_tag="ACG8OL_001339"
     CDS             complement(3492..4685)
                     /locus_tag="ACG8OL_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4817337.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4872..5516)
                     /locus_tag="ACG8OL_001340"
     CDS             complement(4872..5516)
                     /locus_tag="ACG8OL_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4817338.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5610..5744)
                     /locus_tag="ACG8OL_001341"
     CDS             complement(5610..5744)
                     /locus_tag="ACG8OL_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS4817339.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5746..7878)
                     /gene="feoB"
                     /locus_tag="ACG8OL_001342"
     CDS             complement(5746..7878)
                     /gene="feoB"
                     /locus_tag="ACG8OL_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS4817340.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7875..8348)
                     /locus_tag="ACG8OL_001343"
     CDS             complement(7875..8348)
                     /locus_tag="ACG8OL_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS4817341.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8749..8973
                     /gene="nrdH"
                     /locus_tag="ACG8OL_001344"
     CDS             8749..8973
                     /gene="nrdH"
                     /locus_tag="ACG8OL_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS4817342.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9132..11291
                     /gene="nrdE"
                     /locus_tag="ACG8OL_001345"
     CDS             9132..11291
                     /gene="nrdE"
                     /locus_tag="ACG8OL_001345"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS4817343.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            11341..12306
                     /gene="nrdF"
                     /locus_tag="ACG8OL_001346"
     CDS             11341..12306
                     /gene="nrdF"
                     /locus_tag="ACG8OL_001346"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS4817344.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12395..13534)
                     /locus_tag="ACG8OL_001347"
     CDS             complement(12395..13534)
                     /locus_tag="ACG8OL_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4817345.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13843..14556)
                     /locus_tag="ACG8OL_001348"
     CDS             complement(13843..14556)
                     /locus_tag="ACG8OL_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS4817346.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14810..15511)
                     /gene="nagB"
                     /locus_tag="ACG8OL_001349"
     CDS             complement(14810..15511)
                     /gene="nagB"
                     /locus_tag="ACG8OL_001349"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS4817347.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15745..17277
                     /locus_tag="ACG8OL_001350"
     CDS             15745..17277
                     /locus_tag="ACG8OL_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS4817348.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17468..18670)
                     /locus_tag="ACG8OL_001351"
     CDS             complement(17468..18670)
                     /locus_tag="ACG8OL_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4817349.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18752..19441)
                     /locus_tag="ACG8OL_001352"
     CDS             complement(18752..19441)
                     /locus_tag="ACG8OL_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4817350.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19547..19915)
                     /gene="rplL"
                     /locus_tag="ACG8OL_001353"
     CDS             complement(19547..19915)
                     /gene="rplL"
                     /locus_tag="ACG8OL_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS4817351.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19968..20471)
                     /gene="rplJ"
                     /locus_tag="ACG8OL_001354"
     CDS             complement(19968..20471)
                     /gene="rplJ"
                     /locus_tag="ACG8OL_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS4817352.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(20675..20802)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20881..21570)
                     /gene="rplA"
                     /locus_tag="ACG8OL_001355"
     CDS             complement(20881..21570)
                     /gene="rplA"
                     /locus_tag="ACG8OL_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS4817353.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21674..22096)
                     /gene="rplK"
                     /locus_tag="ACG8OL_001356"
     CDS             complement(21674..22096)
                     /gene="rplK"
                     /locus_tag="ACG8OL_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS4817354.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            22439..23107
                     /gene="sdaAB"
                     /locus_tag="ACG8OL_001357"
     CDS             22439..23107
                     /gene="sdaAB"
                     /locus_tag="ACG8OL_001357"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS4817355.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23120..23992
                     /gene="sdaAA"
                     /locus_tag="ACG8OL_001358"
     CDS             23120..23992
                     /gene="sdaAA"
                     /locus_tag="ACG8OL_001358"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS4817356.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24083..25183)
                     /locus_tag="ACG8OL_001359"
                     /pseudo
     CDS             complement(24083..25183)
                     /locus_tag="ACG8OL_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(25275..26162)
                     /locus_tag="ACG8OL_001360"
     CDS             complement(25275..26162)
                     /locus_tag="ACG8OL_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4817357.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(26239..26784)
                     /gene="nusG"
                     /locus_tag="ACG8OL_001361"
     CDS             complement(26239..26784)
                     /gene="nusG"
                     /locus_tag="ACG8OL_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS4817358.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26885..27055)
                     /gene="secE"
                     /locus_tag="ACG8OL_001362"
     CDS             complement(26885..27055)
                     /gene="secE"
                     /locus_tag="ACG8OL_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS4817359.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27079..27231)
                     /gene="rpmG"
                     /locus_tag="ACG8OL_001363"
     CDS             complement(27079..27231)
                     /gene="rpmG"
                     /locus_tag="ACG8OL_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4817360.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            27393..28037
                     /gene="cbpA"
                     /locus_tag="ACG8OL_001364"
     CDS             27393..28037
                     /gene="cbpA"
                     /locus_tag="ACG8OL_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS4817361.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
BASE COUNT         8550 a         5762 c         4886 g         8855 t
ORIGIN      
        1 agaacaacaa acatttgaat gattaaaaat attaagacac caaacacaaa atgatcaaat
       61 acttgatatt gatgagcaac ataagaaaca aataaaccaa ttgtaagtaa ataaatcatg
      121 aatatagcag taagtttaga actggactgt tcaacaatcc tatttatagc taattttcta
      181 tataaccaaa taaacatcag caaaaacaaa gaatcaagca ttatctggaa tactacataa
      241 ccataccctt ttatcccttc aaatgaaaac atatacgtca taaccatgga agtaataata
      301 gatctgaaat catcaataag catacataat agtaatttat ttaaaagcac tatattagtt
      361 ttcttctttt cctttaatat aaaataactt ccgattatag gtatagctgc ggaaacatca
      421 gtaaataatt caacaaaaaa agtgagtggt atcaataaac atacaaaaag atattttctt
      481 ttgttaagat cagtattagt catataaaat aagagaaaaa agaaaaagtt tcccacgaac
      541 attcttaata gataagcttt tgtatcgata gtaatcaaga gatcccctcc tgattttagg
      601 atatcataaa aaaaatttta gttacacttt ttaaataatg aaaaaacaca ttccactaaa
      661 tttgatgccg gttttccttg aggttttgtt ccagcacctc ctttaatttt tttaagctct
      721 tgatctgaac attgtttagc atttagtcta tttttttctt ccattacgat tccccctaca
      781 aactataaat acttttatgt cactgattca atatacctat ttttctaatc aaaaaatttt
      841 gagacatatt taatattata agtatcatta aaattgagat ttatctccat aatctgctcg
      901 atataaacca tttattccag cagttcttcc aatttcactc aaatttctta ttaattcctc
      961 ttgatattca gtgaatctta ttttattagt ataagcagta aaatatagat aattcaccaa
     1021 tctatttatt aaaggaccgg gatctaattc atatttttgt ttttctaatt ttaaatatac
     1081 cttttgtaaa acctcaagaa tatcagaata tttaggatct ttacttatag atatatcatt
     1141 atataattca tgaacaattt gcttagcatt ttttttcatt ttagcacttc cctggaattg
     1201 ctccacctaa aaaaccacct atagacattc ctgcaataca agtagttgcc ttggcccaat
     1261 cgaccgtaca tttattttta gtgcaataca ctccatttcc ataatatttt ccactatgag
     1321 tggtaccccc atagataagt tgtgtctctt taatagacaa aatttttaaa tgtttcataa
     1381 tctaatcacc cctaaattaa tgtttttaga ctattattct aacattcatt aaatttcaaa
     1441 aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa taagaattta atcactttta
     1501 agtatataaa ctatgtatat tctttaatat tttttgagaa tgcacttcgt acataataaa
     1561 ctttaatcac attagtcttt catacctacg atcgatctct ttgactcgca cccataaatt
     1621 cataatagtt tgtcggcgtc tttggacgtt tcgaagatga gctacgcatt ctcttttttt
     1681 atagacaaaa aagatagaga cgaacatgga gcttccaaat ttggatattt gatccaattt
     1741 tggaagctcc atttatttcg tgtctgtctt tttatttttt ccagctttcg actaattctt
     1801 ggttctcttt gatccagtct tttgctgctt gctgtggatc ttttccattg ttgatttcca
     1861 acataacttt ttccatatct ttttctgacc aatggaaatt gtctaatact ttgtaagctt
     1921 ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg gatctgctct tcgcttccca
     1981 ttccgttttc aggatcttct aagtatttca gatcatattt ggcaaacatc caatgaggtg
     2041 tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc ttgacctagt gcaacggtca
     2101 ttgctcctga agaagatgtc tctactttcc agtctttcaa gttgtcatat ttttggatcg
     2161 tgttttcagc agccgtaact acaccagctc ctggctcgat tcctatgatt ttcttacctg
     2221 cctgatcggt taactcatcg atcgaattta catccatgta ggctggcact gctaaaccaa
     2281 cttttgctcc tgtcaaattg gcacctaggt cttcgacttg atctttatat tgtgcatatt
     2341 gtgaaccatg agttttaggt agccaagctg aaaccatggc atctgactcg ccatttgaaa
     2401 cagatttcca catgattgaa ttatctaatg gcgtcatcgt tacatcatag cccatttgtt
     2461 tcaatacttc accgactaca tttgttgaag caacttctgt atcccattca acataagata
     2521 agttgatacg ttttgtttct ttggcagatg aaaaactccc tataccaatc gcacctaaga
     2581 tcactactac tacagcacca atgatcagtc cttgacgttt tttacttttc tgtgttgttg
     2641 ctcctgcagc ttttttattg tttggctggt tcaaatgctg tgtaaaacgg tcaatgatga
     2701 ttgctaagat aactaatgca accccgttga caaatccatt cccgacttgt gctctttgca
     2761 atgctgataa tacgccgcgt cctaagccgg gtgcaccgat catagaggca ataacgacca
     2821 ttgaaagtga cagcattgtt gtttgattga tccctgccat gatcgtactt ttagctaaag
     2881 gcaattctag tttgaatagt ttttgacgac ttgtgctccc gaatgaatca gaagcttcta
     2941 ctaattcttt aggaacttgt cgaatcccta aattcgtaaa acgaacggtt ggtggcagag
     3001 caaagatgac tgaggcaaat acccctggaa ccatcccgat accgaaaaat gcaactgctg
     3061 gaatcaaata aacgaatcct ggcattgtct gcataaagtc aaggatcggt ttgataatac
     3121 tttgggcttt actgcttttt gccatcaaga tccctaatgg taccccaatg atgatcgaga
     3181 tcacactaga taaaagaaca agtgtgacag tattcatcaa atcattccat agaccttggt
     3241 tataaataaa ccatagaccg attaatgtga acaacgtaag tcctggacgt ttcttagaaa
     3301 taaagaaagc tgctaccgtc aataacaaga taaacaaagg agctggaatc actaacagta
     3361 aatttgtaat ccccgacatc actgcctgtc caatcgtttg taaaaatgaa aataggccag
     3421 aaaaagttgt cgtaaaccat tctgtgatat tttctaccca gtttgccact ggtaattgat
     3481 agttcaatag attattcatg ttcactcacc tcgcttgttt ctgctaatgc ttcaatcaca
     3541 ctacctcgga tgacgacacc ttttaatttt ccatcctcta caactgcaag tggagtcggt
     3601 gagtcataaa taatattgaa aatatcactt accaccatgt ctttcccaat caccgtcaca
     3661 ttttcatcta ggaagtcgat caatggacgt ttttgcttcc tagcttctaa cgcttgatct
     3721 gcagtgatga tccctttcag gtgtctcttt ctgtcaacag ccatcagcat gctgacttct
     3781 tcattacgca tccttgtcaa tgcaacgttt ggtccatcag attcgatatt cgtcgtcaat
     3841 gcaggtacca tgatgttttg agccgtaagc actttggaac gatcgacttc ttccacaaat
     3901 tcacgtacat aatcatttgc tggattggtc aatatttctt cacctgttcc gatctgcatg
     3961 atttcgccgt ctttcatcaa ggcgattctg tccccaatcc gtaaagcttc attcaaatca
     4021 tgggtaatga aaataatcgt tttttgaaca ttggcttgca ggtccagtaa ttcgtcttgc
     4081 atttctcgcc ggatcagcgg atcaagagcc gagaacgctt cgtccatcag caagatctca
     4141 ggatcatttg ctaaagctct ggctaggcca acccgttgtt gcatcccacc agataactga
     4201 ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt ctaatgcttt ttctgctttt
     4261 tcttgtcgtt cttctttagg tacaccacgg acttctaacc cgtattctgt attttccaag
     4321 atcgttcgat gtggaaacag accgaagttt tggaaaacca tattgatttt atgacgacgg
     4381 acttctcgca attcttcttt gcttaacttc gatacatctt ctccatcaat atagatactg
     4441 cccgaagttg gctcaatcag acgattcaat agacggatca atgtggattt cccactcccg
     4501 gaaagcccca tgatcacgaa gatttctcct tcttttacgt caaaattcac gtcatacaca
     4561 ccaaccgttg caccggtttt ctttaaaatc tccgttttcg gctggtgatc attcatcata
     4621 tctaaagcgg cttgtgtctt tttaccaaat atctttgtta aatgttttac ttctactttt
     4681 accaaacaaa ttctcctttt ttatgtaaat tcatttttct ttctgaaaaa agtgaccaca
     4741 ctatgttaac atcatgtagt cactttgtca aaaatgaaag aaatttgctt gtccaaactg
     4801 aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc caagttaata tttttttaat
     4861 atttcacaca atcattctgc aagctgatgc tccgggtaaa aaaagtttgt catacgctgt
     4921 agtgtatatt catttccaat aaaatgaacg atgtcccctg ccgtaaattt agcatagggg
     4981 ccaggtgaaa caagcagttc ttcattatga gaaattgcga taactgtggc gccagtcgcc
     5041 tgccagatat tcaactcgct aattgaacgg tccagatgca ttgctttttc tgataaagat
     5101 aattcaaacg gaagtaaagg attcatctta tgtattcgct tcgtttgatt gaccagctga
     5161 tccaataatt gagaaaaatg attcagctca tcttgttgct tctccacact tgccatgatt
     5221 tctgcttttg tatcttggat ggattgtaca tcttgatatt gcaataggaa atcttttgct
     5281 ttttccttcg aagcaacaaa tgccccactt ccatgccgta cttccatgat tccaagatct
     5341 accagcacat tgatagcctt gcgggtagtc tcaggagaag cattgtacgt actggcaagt
     5401 gttgagcggg catggatctt ctcacccact ttatatcggt tttcaacgat acgttcggct
     5461 aaatcaacag caatctgctg gtagcgcggg cggacgattt ttttctttcc agacattttc
     5521 tttcctcttt ctttttttac tttccctcat cttactatgc tctagcaaaa taagcaaaaa
     5581 aggggagaat agcctcttgc aacgactgat catcgcttga tctgttcctt ttttactggg
     5641 caagctgttt gacagtcatc acaattctta ccggatttta aacgagtata gacaattgcg
     5701 cctgcacttc caaagattaa cacacttaat aagaatgtcg ccatcctatt cctctccttc
     5761 taatgacaat atagtataaa actggttctt ggattttggc tgacggaaca aaacataaac
     5821 gagtcctatc atcagtaaaa tagcaagata cgtccctaac gctacctgtc caccctccaa
     5881 taagtgtccc aactgataaa tcacaaaact aactaaataa gctaaaccgc attgatagcc
     5941 tatggcgatc caagtccatt tcttggcatt catttcccga tggatagccc caattgccgc
     6001 aaaacaaggc gcacaaagta aattgaaggc taacaatgaa taagctgctg cgggagtata
     6061 tgtgtgttgt agcacttgcc agatttctct gccattttcg gatacttccg catgaccaaa
     6121 caaaatacca aatgtgccaa taatattttc tttagcgacc aatcctgtaa tcgctgcgac
     6181 tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca actgccagga ttcgcccgaa
     6241 aaaggctaag atactttggt cttcagaaac aggttgagca taaaaattat aagaagagct
     6301 gaaccagatc acgatactag aaacaaagat aatcgttcct gcttttttaa caaaagactt
     6361 gctgcggtca taggtttgtc ttaggaccgc tgaccattga ggaaaatgat atgcgggcaa
     6421 ttccatgata aaaggagctg tatctccagc aaacaaacgt gtttttttca agctgattcc
     6481 agagaaaata attgcagtca taccaagaaa ataggcagaa ggagctaccc aagattgatg
     6541 aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa
     6601 ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt tcaatcgtgc gactggccat
     6661 gacgcctggc actccacatc ctgtagcaat cagcatagga atgaaggatt ttcctgaaag
     6721 accgaatttc ctgaaaagtc gatccatgac aaaagcgatc ctcgccatat atccgcagtc
     6781 ttccagaaag ctcaagcata aaaacaatat aatcaattgc ggtagaaatc ctaacactgc
     6841 tcccacgcct gcgatgatgc cgttcaagat cagctcctgc atccatcccg ctacttgcca
     6901 ttcagctaac aatctgccaa catgttctgg taaccatgta ccaaacaatg tgtcattgat
     6961 ccaatctgtc cccattgttc ctactgtttg aatagccaga taatagatca gccacatcac
     7021 aaatgcgaag atcggcaagg ctaaccaacg attcgttgca atctgatcga ttttgtcact
     7081 catcgataat tggaaagaat catttgacac aacacagagt gtacatagtt gggtaataaa
     7141 ttcataacgt tcattgacca gaatcgtctc actatcgtcg tccaatagtt tctctgttaa
     7201 ctggatcagt tgctcgatct ctttttgctg agaaactgct atctcgtaat cctctcttac
     7261 cttctgatct cgttcaaaat atttcattgc ataccagcgt ttcttgccgc tttgttctcc
     7321 tagtacatct tcaatttcat gtaaagctgc ttctaaacgt ggatcatatg tacagggaac
     7381 aggaatagaa ggccgctttt ttgcagactc ctgaatcttt tcgattagtt tttcgattcc
     7441 ttgctttttc aatacgctga ttggtaaaac tggtacacca agaccatagg aaagcttttc
     7501 tagattgatt ttttttcctt cttttttgat gacatccatc atattcagac caatgatcat
     7561 aggaatgtct aaatccaata actgcgtagt taaataaaga tttcgttcta aattggtagc
     7621 atcaataatg ttgatgatca gttcaggctg ttcctgaacc aaataatctc ttgcaatcac
     7681 ttcttctggt gtataaggag acaaagaata gatgccgggt aggtcctgaa tatgaatgtt
     7741 cggatttttt ttcaacgatc cttctttttt ttctactgtt acgcccggcc aattccctac
     7801 atattgattc gaaccagtca agagattgaa caagctagtt ttcccgctgt ttggatttcc
     7861 gactaacgcg atattcattc tttttcctcc ctttgtatca agattagttc ggcctctgac
     7921 tttcgtaacg tcagttcata ccctcgcact gtcagttcga ttggatctcc cagaggggct
     7981 agttttcgga taactatttt tacatttcgc gtcaatccca tatccattaa ccgtcgcttc
     8041 actgcgcctt gtccgtggat tgctacgacg atcccagtct ctcctgcttt taattgatca
     8101 agagaagtcc agctttcaga ggaagcagtc tttttattga caaagatttg acctagtatt
     8161 tcccgactta ctgccactcg atgacttttt accagtaaaa tggcattgtt gcctgataaa
     8221 ttgacgacga cgattttact tccggcaacg atcccgatat tttgcaaatg gcgttttgtt
     8281 ttttcttctg tttcgattcg ttcgacttga taaacgaaat tctgctttgc ctgcgctaag
     8341 gtctgcatat tgattcctct cttgtgaaat aaatcatgta ttaaaatcat tcattatatg
     8401 ttctaatttt cttacgtgtt acgcaaataa atgagaatca ttctcacata gaatactctc
     8461 ggtaattgag aatgtcaatt atacttgtga tattatgagc gaatctttag tattgataga
     8521 taaaaatgag aaaaaaatat ttcttcatag ttttccacaa ttttttctct attaaatttg
     8581 ctattttccc acacgatact tgttttcagc tcttttttca aaaacacaaa acttagtatt
     8641 ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg acttttttac taagagtttt
     8701 atactatcta ttatagataa taagaatgtg ttagaggagg cactcaccat gaacgtaaaa
     8761 atcttttcta aaaataattg tatgcaatgt aagatggtca aacggttttt aacagaaaat
     8821 caaattgctt ttgaagaagt caatatcgat gaacagcctg aagcagtaga ctggttgaaa
     8881 gcacaaggat tccaaagtgt tcctgtcatt acttctgatg cagaaacagt tatcggattc
     8941 cgtcctgatc agttgcgtaa acttgccagc taacatgact aaaagaatca aaacgtgata
     9001 catggatttg aagggcttga gacagcacca gtttttgtcg caaacttcca aatccttttt
     9061 taaagcttaa aaacaatgca atgatttgca atcaagcatc atcaaatacg aaatgaaggt
     9121 tgggcgtacg tatgagtctt aaaaatttac aagatgtcag ttatttcaag ctgaataatg
     9181 aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa ggataaagaa gctttagttg
     9241 ccttttttga ggaaaatgtc aaaccaaata cgatgacttt tctatctatt atggaaaaaa
     9301 tcaattactt gatcgatcaa gaatatatag aagcagaatt cattcaattg tattcaccat
     9361 catttattga aaagctatat caatttttgg acgagcaaca tttttctttc aaatctttta
     9421 tggctgctta caaattttat tctcaatatg cgttgaaaac caacgacggt tccgcttatc
     9481 tggaaactta tgaagatcga gtagccttca atgcgcttta ctttgcgaac ggggatgaag
     9541 aattagcact tgctcttgca gacgaaatga tccatcaacg atatcagcca gctacacctt
     9601 ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag
     9661 tcactgacga tatgaactct atcgggcgct caatcaactc tgctttgcaa ttgtcccgta
     9721 ttggtggcgg cgtagggatt actttatcaa atcttcgtga agccggcgct cctatcaaag
     9781 gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa attattcgaa gatagtttca
     9841 gctattctaa tcaattaggt cagcgtcaag gagctggcgt ggtttatttg aacgttttcc
     9901 atccagacat tattgctttc ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg
     9961 taaaaactct ttctcttggc gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa
    10021 acgaagatat gtatttattt agtccatata gtgtcgaaaa agaatatggt gtcccattct
    10081 cttatgtaaa tattacagct gagtatgatc atctagttgc aaatccaaac attcgcaaac
    10141 aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa attacaacaa gaatctggct
    10201 atccttacgt aatcaatatt gatacagcga accgcgagaa tccaatcgat ggcaagatca
    10261 tcatgagtaa tttatgttca gagattttac aagttcaaac gccttctgtg attaacggca
    10321 aacaggaata tgaagtatta ggaacggata tctcatgtaa cttaggctcg acaaatatcg
    10381 tgaatttgat gcaaagccct aactttggtc aatctgtgcg tacgatgact cgtgcattga
    10441 catttattac agatgcatct gatatcgatg tcgtaccacc gatccaaaac ggaaataagc
    10501 tgaatcatac gattggatta ggtgccatgg gcttacacac ttatcttgca aaagaacaaa
    10561 tggaatatgg ctcagaggat tcattagact ttacaaatat ttacttcatg ctgttgaatt
    10621 attggacact tgttgaaagc aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt
    10681 ttgaaaaatc agcttatgct gacggcactt atttcgaaaa atacgtaact ggagaattcc
    10741 agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat ttttatccca actgctgaag
    10801 attggaatgc tttgaaacaa gcagtaatga aagatggact gtatcatcaa aatcgcttgg
    10861 cagtagcacc aaatggctca atttcatata tcaacgatac gagtgcaagt attcatccta
    10921 tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg taaaatttat tacccagcgc
    10981 cttatctatc caatgagacg attccgtatt acacttctgc ttatgatatg gacatgcgta
    11041 aagtaatcga tgtttatgca atggcacaac aacacgtcga tcaaggcatg agtttgactt
    11101 tattcatgcg ttctgaaatc ccagaaggca tttatgaatg gaagacaaca acgaagcaaa
    11161 cgacacgtga tttgaatatt ttaagacatt acgctttcca caaaggaatc aaatccattt
    11221 attacgtacg tacctttact gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct
    11281 gcgtgatcta atatcataga tagattcgct tattcatatt ttaattaaag gagcattctc
    11341 atgtcagaaa catattatga agcaataaac tggaatgaaa ttgaagatat cattgataaa
    11401 tccacttggg agaagctgac agagcaattt tggctagata cacgtatccc actttcaaat
    11461 gacctagatg attggcgtac attgtctcaa ttggaaaaag atacagtagg acatgtattt
    11521 ggcggtttga cactgctaga tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa
    11581 gatgtccgca ctccccatga agaagccgtt ttgaacaata ttcaattcat ggaatccgtt
    11641 catgccaaaa gttattcttc tatttttagt acactgaata cgaaaaaaga aatcgaagaa
    11701 atttttgact ggacaaatac gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa
    11761 atctacaaac acggtacacc gctagaaaag aaaattgcca gcgtcttttt agaaacattc
    11821 ttgttctatt caggttttta tactcctctt tattatttag gaaacaacaa attggctaac
    11881 gtagcagaga tcatcaaact aatcatccga gatgaatctg tacatggaac ctacatcggt
    11941 tacaaattcc aattaggatt caacgagtta cctgaagcag aacaagaaac gttaaaagac
    12001 tggatgtaca atcttcttta tgagctttac gaaaacgaag aacgttatac agaagaatta
    12061 tacgatccaa tcggctggac agaagaagtc aagacattcc tacgctataa tgccaacaaa
    12121 gcattgatga acctagggat ggatccactc tttccagata ctgccaatga tgtcaaccca
    12181 atcgtaatga acgggatttc aacaggcaca agcaaccatg acttcttctc acaagtcgga
    12241 aacggctatt tgttaggtca tgtagaagcg atgaaagacg acgattattt gattggattg
    12301 gaataaggat ttattttaat tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag
    12361 atattttaaa tgtacactga actctttttt atgcttattc acttgtttct tccagcactt
    12421 ttggactgtc taacttttcc gaaacatgtt ccattcttct caataaatag attttccgga
    12481 tatttaatat tatcgtctta atcactgcat agacaggtat gcaaatcagc atgcctaata
    12541 ttcctgcgac acttcctgct ccaatcaata ccaagatgat cgtcaaagga tggatattca
    12601 ttgatttccc aaataacaac ggcttgatca aatttccatc taattgttgg atcactaaaa
    12661 tcgacaacgc catatacaaa gcttgaaatg gagagataaa caaaccgaca atgattgctg
    12721 gtaccgctcc aataaaaggg ccgacatacg ggataatatt cgtgattccg caaatgaccg
    12781 caagcaacaa gctataaggc tgatcgaata ttctcattgc caaaaagctc attacaccaa
    12841 taatcaaagc atctaagatc gtactgctga tataggcaga taatgtttcg tttaattctt
    12901 ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa
    12961 taaatttgtg cccatccttg aacataaata gcaaaataaa tggaacggta aataacaaaa
    13021 taaaaaattg catcataaat gaaatgattt ttgagacact tgcagataaa ctgactaata
    13081 tgatattgaa caaattacta agagataaat ttgctgatgc taattgttct tccaaattga
    13141 aatttttaaa ctcttcccgc tgcgccaatt catttaacca tttgcttgtt tcatctgcat
    13201 acattggcaa gctctgggtc aattgaattg tctgttccat taactgaggc actacattca
    13261 tgacagttaa aacaactaaa atgattacga ccaaaaacgt caataaaaaa ccgaacactc
    13321 tcgggatccg atgcttttcg agaaaaacaa cgattggatc gaacatataa tagataaaac
    13381 cagcaacaag caatggtaaa aaaactgccg aaagcatttt caatattgga ctaaaaatat
    13441 tcggcatttg taaaataaag tacacgccga tgatcgtgag tactagctcc actgtccaga
    13501 aaaataattt tgattgttta aaacgtgatt gcatacatct cctccaaggt cctttgatac
    13561 ttctattcta tcatttaaca aagagttacg cgctgagatt ttcatttgct taaaaaaaac
    13621 aaaaaaaata aacatatttt aatgagtaga cgagtactta aacaaaaaca cggtttggag
    13681 tagtgacaaa aattatactg cagtcccttt ttccaaataa acggtgttca aaagcttgca
    13741 tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac tatttttatt tggctgggct
    13801 tattacttga tgtaggacat ctattttaca acctcagatc atttattgcg caatactgac
    13861 taaatgcaac tttgtgatat ttttgtgtac ggacaactcc tcgatcacat cggcataagt
    13921 gagccctttt ggaagatcaa tcgtatagat atttttgtac actcggtagt catcaacgaa
    13981 ttttacgtca aaattcacat cttcgattac gatatttttc tcttcaaaat aatcggtaag
    14041 aaaatctttg gtttcttttc tatggataaa ttggatttct aactttttag tcgtaggtac
    14101 atggatcacg cgtttcacta ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc
    14161 aatggaatag taacccatgc cgatggcgat ccctaatcca gcaattgccc ataaagaagc
    14221 ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa
    14281 accgacacca ctcaccactt gagcaatcag ccttgcttca tctgaacgaa tcgttccagc
    14341 aagctgtgga tactctaaag catctcttaa tgcaccacta gcgatctcaa cttggatcaa
    14401 tgcaatgata catgctccca tacaaactaa aatatgcgta cgcatacctg ctggacgatt
    14461 tttatattgt cgttcaaaac cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa
    14521 aataatctct ggaattgtca ataccatatc attcatctaa acaccctcca gtccaatata
    14581 tcatgtctga aaggagcaga caaaaaaagt gcaattttta cctcttacga aaaaaggact
    14641 gtgacaaaat ttttgtcata gtccttttta tgaataaacg gtgttcaatc agctgaccct
    14701 cggtattact caacaaacgg caaaatatgg ggagctattt ttcctttgtc ttctctaata
    14761 ctcaggtcaa aacgctgatt tcacaacctc tttagcctac tattgaaaat tacaacttac
    14821 ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg attttgcaat acacttgctg
    14881 gcaaatgatt cgttacaggt ccttcaatca tccctttgat tgcatcagct ttgctttcgc
    14941 caaatgccat caagacgatt tctttacttt tcataattga accgattccc attgaaacag
    15001 cttttgttgg tacatcttct gctttatcga agaatcgttt gtttgcattg attgttgatt
    15061 ctgttaagtc aactactgaa gtcgttccgt caaatgaagc accaggttca ttgaaaccga
    15121 tatgtccatt ttgcccgatt cctaagattt ggatatcgat tggatgtgca tcgatgattt
    15181 gatcatactt tttgcatgct gcttccagat cttctgcttt tccatctggt acaaacgttt
    15241 ctttgaatgg ttttttatca aataattgga catccatgaa atgacggtag ctttgttcat
    15301 ctgaaccgcc tagtccaacg tactcatcta agtttacaga agtcatttca ctgaaatcca
    15361 aatcgcttga agtcatttct ttatataaag taactggtgt gcttcctgtt gccaatccta
    15421 atacttttgc acctttgttc ataccttctt tgatgatttc aaatgctttt tttccgcctt
    15481 cttcagcgtt ttttacacga atgatttcca tagtgattgc tcctttatat tttggtatag
    15541 cccaattata gcattctttt tttcttttta caataattag tatagaccaa attgcatttt
    15601 tttcattttc ttgacaataa ataggaatcc gtgttaggtt tcataagatg gttcaatcaa
    15661 acaatccatg ttgcatgaaa accaagcaag tgattcactg tggacgcttt atgtctgcag
    15721 tttttttatg aaaaaggagt tattatgagt atactgacat taaacaacat tacccagcaa
    15781 tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt
    15841 gggctgattg gacaaaacgg agccggtaaa agtacattga ttaaaatcat cactggtgag
    15901 atcttaccag atgacggaca gatccattgg caaaaaaata ttcacaaagg ttacctcgat
    15961 caatatgtgg aagtcgatga aacattaaca atcgaagaat ttctaaaaac tgcgtacgca
    16021 aaagaatacg aaaaggaaga aaaaattgct tttctttacc aaacatatag cgagaccttc
    16081 gatgattcct tgctggaaaa agctgggaaa ctccaaacgg aattagacca aggagtgttt
    16141 tatcaaatcg acaccttagt tgctgagatg agcagcgggt tggggatcga tgtcttaggt
    16201 ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa
    16261 ctacttttag aaaacccaga tgttctgatc ttagatgaac caaccaatca cttagatgat
    16321 cagcatatcc aatggctgac gcagtttctt caggattttg acggtgcgta tatcgtgatt
    16381 tcccatgacc aagaatttct tgatcagatc accacccata tcgcagacat cgagtttgga
    16441 aagattacta agtatactgg ccatctaaaa caagcattga aacaaaagga acaaaatcgc
    16501 gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat
    16561 attcgaaaat acaaagccgg ttcccgatca acgatggcaa aaagccgaca aaaacaatta
    16621 gataaaatcg aacggctgac gcctccagct tcagcaagta aagctgtctt tgattttcca
    16681 ttctcgccta ttgtcacgac attagcagtg gaaacaactg atttagtcat cggatatgat
    16741 cgaccgctac ttgcacccat caatttgacc atccgctttg gtgaaaatat tgccattcga
    16801 ggcttcaacg ggattggaaa atctacttta ttaaaaacac tgatcggtga gattgaaaaa
    16861 ataagtggcg atttccactt cccagataac acaaaaatca attacttttc acaagatctg
    16921 tactgggaaa atccattaga aacaccacta caatatttaa gcagtcaatt tccaaaagcg
    16981 actatcaaag aattacgtcg ccaactagct aaagcagggt tagttaatca attagcaagt
    17041 gaacctctca ctacattaag tggtggggaa caaactaagg tgaaacttgc acagttgaca
    17101 atgaaccaag gtaatttact gattctcgat gaaccgacga accatatcga tcaagaaacg
    17161 aaagaaagct tacaagacgg gatacagaaa tttccaggaa cagtaatcat cgtctctcat
    17221 gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg agattgaacc aaaataaacg
    17281 tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc
    17341 tgaataaatg gtggccaatt agctgacctt cggtattagc tcaaaaaacg gcaaaatatg
    17401 aggagctatt tttatttggc tattcttatt acttgatgca gaacagcttt tcacaacctc
    17461 ttttcgtcta ttttttattt tcgagatttc tctttccttt aaaaacaact gtttgaaaga
    17521 agaacgcaca tccttgaaaa acaagcccgc taaccgttac acctaaccag ccatactgct
    17581 gccacattgt cgtccctagc aatgaaccag ccgcacctcc aataaagtac ataaacataa
    17641 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
    17701 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
    17761 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
    17821 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
    17881 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
    17941 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
    18001 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
    18061 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
    18121 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
    18181 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
    18241 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
    18301 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
    18361 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgctg
    18421 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
    18481 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
    18541 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
    18601 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt tttttttcat
    18661 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
    18721 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
    18781 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
    18841 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
    18901 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
    18961 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
    19021 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
    19081 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
    19141 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
    19201 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
    19261 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
    19321 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
    19381 ctaccagtat cagaatgatt cccaacgaga tcaatatcaa tcggattatt ttctttttca
    19441 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
    19501 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
    19561 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
    19621 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
    19681 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
    19741 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
    19801 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
    19861 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
    19921 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
    19981 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
    20041 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
    20101 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
    20161 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
    20221 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
    20281 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
    20341 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
    20401 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
    20461 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
    20521 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
    20581 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
    20641 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
    20701 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
    20761 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
    20821 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
    20881 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
    20941 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
    21001 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
    21061 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
    21121 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
    21181 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
    21241 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
    21301 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
    21361 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
    21421 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
    21481 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
    21541 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
    21601 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
    21661 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
    21721 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
    21781 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
    21841 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
    21901 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
    21961 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
    22021 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
    22081 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
    22141 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
    22201 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
    22261 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
    22321 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
    22381 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
    22441 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
    22501 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
    22561 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
    22621 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
    22681 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
    22741 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
    22801 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
    22861 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
    22921 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
    22981 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
    23041 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
    23101 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
    23161 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
    23221 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
    23281 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
    23341 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
    23401 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
    23461 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
    23521 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
    23581 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
    23641 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
    23701 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
    23761 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
    23821 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
    23881 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
    23941 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
    24001 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
    24061 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
    24121 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
    24181 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
    24241 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
    24301 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
    24361 acgcaggacc tccatctagt tgacgtccac catgattgga tacccagata ccatctgccc
    24421 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
    24481 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
    24541 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
    24601 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
    24661 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
    24721 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
    24781 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
    24841 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
    24901 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
    24961 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
    25021 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
    25081 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
    25141 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
    25201 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
    25261 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
    25321 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
    25381 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
    25441 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
    25501 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
    25561 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
    25621 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
    25681 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
    25741 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
    25801 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
    25861 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
    25921 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
    25981 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
    26041 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
    26101 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
    26161 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
    26221 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
    26281 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
    26341 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
    26401 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
    26461 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
    26521 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
    26581 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
    26641 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
    26701 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
    26761 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
    26821 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
    26881 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
    26941 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
    27001 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
    27061 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
    27121 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
    27181 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
    27241 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
    27301 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
    27361 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
    27421 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
    27481 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
    27541 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
    27601 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    27661 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    27721 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    27781 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    27841 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    27901 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    27961 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    28021 aaaaccaaga agcataatca aaaagcgaaa aaa
//
LOCUS       DAZUJO010000029        26827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000029 DAZUJO010000000
VERSION     DAZUJO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.029"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..244
                     /locus_tag="ACG8OL_001365"
     CDS             5..244
                     /locus_tag="ACG8OL_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817362.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            311..550
                     /locus_tag="ACG8OL_001366"
     CDS             311..550
                     /locus_tag="ACG8OL_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817363.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            636..1307
                     /locus_tag="ACG8OL_001367"
     CDS             636..1307
                     /locus_tag="ACG8OL_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4817364.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1360..3336
                     /locus_tag="ACG8OL_001368"
     CDS             1360..3336
                     /locus_tag="ACG8OL_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4817365.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVINGTGAEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDIQLTYNMFVNDSAAIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3349..4092
                     /locus_tag="ACG8OL_001369"
     CDS             3349..4092
                     /locus_tag="ACG8OL_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4817366.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPSVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIEEVQ
                     KDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEENRKQ
                     KLSWKRLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4108..4881
                     /locus_tag="ACG8OL_001370"
     CDS             4108..4881
                     /locus_tag="ACG8OL_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS4817367.1"
                     /translation="MRNRKWLLVCAVALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKKLSYDQLKKVAEGETKTI
                     DDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLY
                     PKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4891..5139
                     /locus_tag="ACG8OL_001371"
     CDS             4891..5139
                     /locus_tag="ACG8OL_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817368.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5182..7281
                     /locus_tag="ACG8OL_001372"
     CDS             5182..7281
                     /locus_tag="ACG8OL_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS4817369.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7319..9376)
                     /locus_tag="ACG8OL_001373"
     CDS             complement(7319..9376)
                     /locus_tag="ACG8OL_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFS4817370.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9490..9702)
                     /locus_tag="ACG8OL_001374"
     CDS             complement(9490..9702)
                     /locus_tag="ACG8OL_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817371.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9889..10356
                     /locus_tag="ACG8OL_001375"
     CDS             9889..10356
                     /locus_tag="ACG8OL_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817372.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFI
                     NKPDSLFIVVPYEKDGVKKIVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            10353..10595
                     /locus_tag="ACG8OL_001376"
     CDS             10353..10595
                     /locus_tag="ACG8OL_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS4817373.1"
                     /translation="MIASLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            10613..10915
                     /locus_tag="ACG8OL_001377"
     CDS             10613..10915
                     /locus_tag="ACG8OL_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817374.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10987..13041
                     /locus_tag="ACG8OL_001378"
     CDS             10987..13041
                     /locus_tag="ACG8OL_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817375.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13041..14873
                     /locus_tag="ACG8OL_001379"
     CDS             13041..14873
                     /locus_tag="ACG8OL_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFS4817376.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            15586..17484
                     /gene="ltrA"
                     /locus_tag="ACG8OL_001380"
     CDS             15586..17484
                     /gene="ltrA"
                     /locus_tag="ACG8OL_001380"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4817377.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            17601..18401
                     /locus_tag="ACG8OL_001381"
                     /pseudo
     CDS             17601..18401
                     /locus_tag="ACG8OL_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            18398..20797
                     /locus_tag="ACG8OL_001382"
     CDS             18398..20797
                     /locus_tag="ACG8OL_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS4817378.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAAEMADNDKSGLSDLQKDRLKALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYRSVNSENEKRISDLSKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPK
                     AQNLPMKDSRLNQFKDNDQSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGN
                     ASVQNVVTREAAPKPIQNVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLKDIK
                     S"
     gene            20849..21181
                     /locus_tag="ACG8OL_001383"
     CDS             20849..21181
                     /locus_tag="ACG8OL_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817379.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            21182..21775
                     /locus_tag="ACG8OL_001384"
     CDS             21182..21775
                     /locus_tag="ACG8OL_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817380.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            21938..23962
                     /locus_tag="ACG8OL_001385"
     CDS             21938..23962
                     /locus_tag="ACG8OL_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFS4817381.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFVKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            23962..25167
                     /locus_tag="ACG8OL_001386"
     CDS             23962..25167
                     /locus_tag="ACG8OL_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4817382.1"
                     /translation="MENQNESLSKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            25183..25827
                     /locus_tag="ACG8OL_001387"
     CDS             25183..25827
                     /locus_tag="ACG8OL_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817383.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            25838..26644
                     /locus_tag="ACG8OL_001388"
     CDS             25838..26644
                     /locus_tag="ACG8OL_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817384.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRITKENI"
BASE COUNT         9704 a         4208 c         5478 g         7437 t
ORIGIN      
        1 ttagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa aaggaaggta
       61 tgtacagctg ttagctcgtg gttattcacc taaagatgta ttcaaatccc tgtctaaagg
      121 aacagatatg gaaaaagaaa gattacgaaa agaattcgat tattggagaa cccataatgg
      181 aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga taacccaaga
      241 ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa caaggaaggt
      301 aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga aagaaatgga
      361 agaaaagttg ctaaaaacga tgaaacaaaa acaccttaaa agattatctg tcatgcaata
      421 tatcaatgat atgcagataa caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa
      481 ctttgaacag ttgagacgaa cttatgtgaa aaaaagttct aattgtcaac tattgttaga
      541 agtctcatga cattcattaa tagaattcat ttttaaaact ttttctatgg aaatgagaaa
      601 aagatttttt gttttgtctg aaagggggta gctggatgag aaaatggtta tttggttttt
      661 taggagtcgc tttaatcgtc ttttgcagtg tgtttggcta tgttagctac caaaaacatg
      721 aaggaaaggt gtttaagcaa aatatagaaa aaaagatgcc cgtagatcaa atcaatgaac
      781 atgcaaagag ttataaggag gatgcaacga acgtcaataa tgatatgtct ttggggcaaa
      841 tgttgtccat acaaaaagaa gcaatcgaaa tgggtgtcaa taaacaagcc tttgcacaaa
      901 ttcagatacc tgcacttggt ttagcattgc caatttttaa aggggcaaat caatacactt
      961 tgagtcttgg agcggctact tatttttatg aagatgccga aatgggcaaa ggaaactatg
     1021 tcctagcagg tcataacatg gaaatgccag gtgttttatt ttctgatatt caaaagttgt
     1081 cattagggga ggtgatggac ttagttagta acgatggcgt ctatcgttac aaggtaacgc
     1141 gtaaatttat cgtacctgag tactttaaat taatcgatgg cgttcctgaa gagaactctt
     1201 tcttgtcttt accaaagaaa ggagagaaac ccttattaac gttgtttact tgtgtttata
     1261 cgtcacaagg aaaagaaaga tacgtggtac agggagagct acaataacaa ggttttaatc
     1321 aacgcgagtc gttgaaatat gaaatgagga gagtaaaaaa tggcaataaa tcggtatgta
     1381 aaaaaagctg gaatgcttgt tggaatgtta ggaatcattg caagctgctt tcaggggtat
     1441 caagtacacg cagaagatgt cacgcaaaaa acgccaccag agaaggtcaa tatcacggtt
     1501 cataaattaa tgtacgatca aggaacgcaa ttgaatgttg atattgacgg gatcaaaaac
     1561 gatggttata cgcacgatca atatcctaca ggtgtaacga aatacaacaa ggcagattat
     1621 ggtgatgttg agtttacgtt aggaaatatt acggatcaag tattaccgag ggaagattct
     1681 gatctaacta acgcgaaagt ggacgaaatc gtgaaagacg tagaagacaa gggatctaac
     1741 agtgagtacg tcaagaatgc aaccaacaaa attacttcag ctgtagatga aaatggtgag
     1801 atcacttttg ctgatcaacc agcctatgtc aattccaaag ggaatgtata tgtggtttat
     1861 gaaagtaaat ccgctgctgg attagtcaca caaaaagcaa aaccaatggt tgtgattgcg
     1921 ccaatgactg ataatacagg ttcaggtttt ttaaaagaca ttcacttata tcctaaaaat
     1981 attgtttcta aattgagttt tgaattaacg aaatttggtg acgatggcac agctcaaagc
     2041 aaacaaacac ctttaaaagg ggcaaaattt gagttataca agggagaacc tggaaaagga
     2101 acaaaattag gagatcttgt atcggatgat caaggaaagc tgactgctac tgatttgacg
     2161 ttaggaaaat actattttgt ggaagttcct tcagaagttg tagtgggatc agataaagaa
     2221 ccaacagcag atcaatattt attaggcgca gacgctcgca acgatgcaca caataaatta
     2281 accttcgaaa ttacgaatga tggcgtgaca agtgacttaa aagcttccta tgtaaattac
     2341 aaagctcctg taatcgataa gacagtaatc aatggaacag gggccgaaca ttctttccaa
     2401 attggtgacg cagtgaatta tcaaggaacg attcatatcc ctacagatat tgcaggtgga
     2461 acagatggga ttacagtaaa tggtgtgaag agtgaaactt ctccttacag cgtatttaag
     2521 tggggagata cagcaggtaa aggactttct tatgtcgctg ctaaagcaaa tatcaaagta
     2581 acaaataaag atggatcagt ggtattaaaa gaaaatacag attataagat ccaaaattca
     2641 gaaaatggct ttgtgatcga ttttatcgtg aataatggcc aagtaagtga tactgtcgca
     2701 agtttacacg gacaagatat tcaactgact tataacatgt ttgtgaatga tagtgctgca
     2761 atcgcaaatc cattaacaaa tagcgtggat ttcgtttata gcaacaatcc attcaatcag
     2821 gaagaacacc atgaaaaaac gaaagccgac gctgtgactt atggcgcaaa attcttaaaa
     2881 gctgatggcg gactatttgg aacaggaatc aaagcaacac cgcttgaagg cgcagaattt
     2941 gctgttaaaa acgcagaagg aaaatattat ggtggattag ttgatacgga taaagacggt
     3001 gtcaaagaag cagtatgggt agacgatgta gcaaatgcag cgatcctaaa atcagataaa
     3061 gaaggtcact ttgaaatcac tggtttaact gaaggtgaat attcactgga agaaacaaaa
     3121 gcaccaactg gttaccaaaa attaacagaa gatattgcat ttaaagtaaa caaaaactca
     3181 tttaaagagg aaaatcgaat cactgttaaa aataatcaaa aagcagctat tccattaaca
     3241 ggtagtaatg gtttccaaac gtatgtattg atctcttgct tgttgctagg agcaggggca
     3301 ctttctgcag ttgtttactt taagaagaaa gcctagaagg atcagctaat gattattcgt
     3361 aagcccccaa aaagaagagt catgcacaaa ctgatgatat ttttttggtt gctgtcggca
     3421 gtagcagtct atttaattcc atctgtggaa acaaatgtag cgaaatttca acaacagcaa
     3481 agaattttag ccattgaaaa tcttgaaaaa acagattctt ctttggatgt tgcgaaaata
     3541 gagttaggcg atcctgttgg tgttcttaca attccaagta tttctttaaa actaccaatt
     3601 tatgacggaa cgtccgacaa aattttagaa aatggtgtag gtattacgga aggaactggt
     3661 gatattacag gaggaaacgg taaaaatcct ttaatcgcag gtcatagtgg actttataaa
     3721 gataatcttt ttgatgattt accttcagta aaaaaaggag aaaagtttta tatcaaagtt
     3781 gatggtgagc aacacgctta tcaaatcgat cggattgagg aagtgcaaaa agacgaacta
     3841 caaagaaact ttgtcactta cttagaacca aatcccaatg aagatcgagt aacgctaatg
     3901 acatgtacgc ctaaaggaat caacacacac cgtttcctag tgtatgggaa acgagtaacc
     3961 tttacaaaat cagaattaaa agatgaagaa aataggaaac aaaaattatc ttggaaacgg
     4021 cttttaggaa gtactgtttt tctttcagtc atgatcattg gttcgctatt tgtttataaa
     4081 aagaaaaaat aaaggagggt aaccaacatg agaaatagaa aatggctact tgtttgtgca
     4141 gtcgctcttt tttctttcac tgcctacaca cagttaacgt ttgcagacag tcaagaaagc
     4201 acggatagca cagaacaaac aatccaaacg tcagatagca gcacgattca aagcagcgat
     4261 gaaaccgtcg attcgtcaac agtggattct tcagaagaac cgaaaattgc tgaagacgat
     4321 caaatagtca aacagaccat tcacgttcaa aagattattt cttcagaaca attagacaac
     4381 caggggcaaa agcttgaaaa acaagaagga gtaaatggcg cgaagtttat tgtctacgat
     4441 atcacgaata tcttagaaga aatgtcacaa gatgataatg agaaaaatca atctgtagaa
     4501 ataattgagt caaaattgaa agatcgagca aaaaaacttt catacgacca gttgaaaaaa
     4561 gtggctgaag gagaaaccaa aacgattgat gatcaggaag gcgtgattga attttccatt
     4621 gaagtacctg caaacaaaaa acaagcatac tatatcgtta atgaatcaag ccctgaaaac
     4681 atttcaaaca gtgaagatat tgttttattg acacctgttt caaatgaaaa aggtgaattt
     4741 ttgacggatg tttggttata ccctaaatca gagaaatcag agccgaaaga agaaattaaa
     4801 aaagttgtgt caactggtgt aaagaagaac ttttttgaaa attgttggga attttttgct
     4861 caattatttt ttgataatta ggatgaaatg atgaaaataa taagaatatt agtttttaat
     4921 ctatcctttt tagccgcaac aatattgtgt tgcggtttaa ttttttctca attatcaatt
     4981 tatccaagat atcaacgatt gttcattcat tttctaaaca ttcaatgtgc ttccatgcca
     5041 ttagaggtga ttaaaaagct cttgatcaat ccaattttta tggttagttt ggttcttctt
     5101 acaggattgg taacgtgggt attaggaaaa aaagaatgaa aagcttacga tcgtttaaaa
     5161 aaatattaga tggagggcat tatggaaaat aaaattcatt gcactacggg aagagtaatt
     5221 actttagtgt tgtttttgtt ccttcttctt tttgcgaggg gaactgcagt ccaggcctta
     5281 gaaaaagttg atccgagtca aatcaaagaa aataataatc tggtgcttta tcatgatagt
     5341 gattcattga gaaatatcga agtaactggg atcaatggat ttaagatact agatgagtat
     5401 tcaactaaaa aacaaaaagt acttgcttgg ccaaaagtca aagatttgaa aagtacgtca
     5461 aaaatcgttg cccattatca aaaagtaggt acttatctag gaaaagaaat tgattgtgac
     5521 gtgacttttt ctaattttgt agcgcacgct ggggttaacc aagacggatt agctaaaaca
     5581 attaatggca aaaagtatta taattatttg ctctttgata aaaatattta tcgaggattt
     5641 ttcatgcagt caatcaatgt catgaatgca aaactagaat ttaagtatgc aggaacaaaa
     5701 acaaaagtag ctttgagtga accacagcca gaaattcaat caaaaatgag atcgatgaga
     5761 gcagctggta ttgatttaaa cgatacattt ctatccattt acagtttaaa tggacagtat
     5821 cagcaacgtg tgtcttggta taatgacaat aatgaatctg tcggtgaacg taatattgat
     5881 atgagagaat ttgttgggta tgaaaaaatg ggtagcttac cttattttgt cacaacagat
     5941 acagcatgtg cagaatacaa agctcctgcg ttatcaacaa acaatttaac ttcaaaagta
     6001 gtgggaggac gtgcagaaaa ggcttatagc tcgaatgatc atttcaccga tgttgtagga
     6061 gctgatactt atcacagaag tggtgtaacg tatacgcttc aaggcgcttc cccaacattc
     6121 atgattggcg caaatacgaa tagtatgatg tttagctttg atactgcatt gctatggaca
     6181 ccacaaccat cgaagcctac aaaagaagtg tttaacaaag ctaatactga agaggcagca
     6241 cacaatattg acaaaaaagt gattccacaa ggatcagatg tttactatca tattcaccaa
     6301 aagtttgatg cattaacagt caacacaatg aacaaataca aatcatttaa aatcactgat
     6361 acctttgaca gcaaaaattt tgatatggta tcggatggga aaaactatga tggcgcattg
     6421 tggtatgttg aagcgaatgg tacttggaaa aaagctgata caggaggaaa gttaactgta
     6481 tcaggtaata cagtcactta tactgcttct gacagctttt tagctaacgc tgcaaattac
     6541 ggtagggatt atattttgca gctccatatg agagcaaaaa atgatgcaaa aggaattgca
     6601 ccaaatgtgg ctactgtaac gtttaacaat gatccgaaca aaggaacaca aaaaacaaat
     6661 acagtagaaa actatttctc tcttaaacca aataaaaaag tggaacaaga tgggaaagat
     6721 gttgatggtc gaaatgatgg taaaaaagga acgcctacag ctcctttaaa tgcaggatct
     6781 gaagtacaat atctagtcac acaaaaatgg catacaaaag gtgtagacat tgcctcagat
     6841 cattacaagc aatttagtat tcaagatcca gtggaagatc gtttgacgta taaagaaggt
     6901 tctgcgcgag tgatagatat gagtacagga aaagatatta cttcagaagg tacactaacc
     6961 tatgatagca attcaagaac gctgaagtgg gaagcttccg ctgatttctt aagtaaaaat
     7021 cttttagatg gacgagaaat tcaactgata tttacagcta aaactccttt gaatgaagaa
     7081 ttggatatcg acaaccaagc aaaagtttca gtagaaagta ttgagaaagc aactaatgta
     7141 gtaacgattg gggtaaaacc taacttacct cagatcattg tgccaaaaac tggatcgaat
     7201 catttagtgc taactgttat tggaagtata ttatttctta ctttagcatc attgggctat
     7261 gtagttttaa aactcaaata aatataaaaa caaggctgag aatcagcctt gttttctttt
     7321 atttgaagat tggattcgca ctaataaata cattatctac gccaattcta ccagcagagt
     7381 caactacaaa attatgacct aatccgagaa tactttggtt attataaagt aaatttgcat
     7441 tatgggcagg gctatttttc catcctacca tggcatcgat agcaccatcg tctgataaaa
     7501 ggtcatcaat atacagccca gtttgactta tgttttctcc cttagcaccg tagcagccta
     7561 agctttccaa taaatcttgg aaagtaccac caccagggcg atagtgactg aagctgactt
     7621 gtaattcttt agctcgaata tctgcagcct gttgaacaat atcattgaat tgcattgggg
     7681 caacaccatt ttgttggcga taatcattta accaagcaag ttctttttga cgcataaagt
     7741 tacgtaattc gtctaatgaa atctttttat atttgaatgt gattgtttta ttttgatcga
     7801 cgttattaat ggatcgttgg ccgtttttat ccacggtaac ggtaggatcg tcatttctgc
     7861 acaagaaata attttgatca gtcatgtttt tccaaggagt agcaaagcta tccccttcgt
     7921 atactgtttt tgattcacta gagagaacag cgttggtatc agcatttaca tgattaacag
     7981 tcacattaaa tttacgagtg tagttgaaaa tataattttg actttcagtc acattgttta
     8041 aggttactga atttgttggt tctacccgat cgtctaaaac atatccttga atagaaggag
     8101 cagttaaagt gacactgtca ccgtgagtga cattgactat ctgttctttc aaaatattaa
     8161 ctcgtccttc gtttttgtag ttgttattgc acaacaaatt gatttgatgc acttgattat
     8221 aattgaatgt gattgttgta ttacctgtga ttgttttgct ttgagtatca ttaccaatca
     8281 ctttgtagct aaaattatct ttagcgcctt cgctatattt aaatgctttt gccttagcag
     8341 tgtaagtaga accagaagtt acttttgctt gatctgtagc aatcacttga cctgtttgat
     8401 tgtcaatatg gttaatagta accgtatatt cttttggtgt ttcaggtttg tgttgcaacc
     8461 ctttaaatgc agcatcaaga ctatttttag cattatttac ttgggcttga gaagccttag
     8521 aatcgtctag tactttttta gcattggata aagcagtctg aaaattattc caagattctt
     8581 ctgtaaaatc tcctttagca gttgatttta ctcggttgta taaagcttgt aattcagctt
     8641 tattcacagt aggaactggt tttttcttta atcctttgta agcagaatct aatttatttt
     8701 tggcactatc cacttgtttt tgagtcgctt ttgagtcatt taacactttc ttagcattgt
     8761 ttaaagcggt ttggaatgcg ttccaggtat tgtcggtata gtcacctttt ttagttccct
     8821 taacgctcgt ataaagagct tctaaagctg atttattcac tttgggttta gaggtgtcag
     8881 cgccatacca accgatacct tcgtatttcc agccatagtc tttggttaac cattgtactt
     8941 ctttccaact tgcagtgtag tgatgtgagc cagattttac accaggaagg aaaaggcggt
     9001 agatcggttt attatttttt tcggcagaac gccaggcagg cccttcataa gtccagccgt
     9061 aatttttagt caaccatttc acttcatccc aactagctgt gtaatggtga tcacgaagac
     9121 ctttattgta caaacgatag acaagatggc caccgctagt cggtgcttcc caggcggggc
     9181 cttcataatt tccccatttt atctttacta gatggtcacg ttcttttttg ctagaggtgt
     9241 aaaaatgttc atgattattt ggattgtaaa ggcgatacat gacgttagtt gaagcagcgt
     9301 caacagatcc agcaagacta agtgaggcag caaatgttcc aatagctaaa acagtttttg
     9361 gattaaataa tttcattttt tcaactccta aatttaatta aaaatgttta catgattatt
     9421 atatatcggc taaccgatat agtcaataaa aaataagaaa agcctgatat aattttggct
     9481 tttcttattt tatttgttag ttgaagtatt aatatcaacg atttcttcag ccaatttatt
     9541 taatttcata gcatttatga atctcatgtt gtctatacta gagcgtttgt tggcaaggtc
     9601 actgactgta gttaagggta cgccagattc tatggatata gaacgaactg atttaccact
     9661 atcaaacagc aaccattcga ttttcttttt atcagcgtac atttcaatgc tccttcttgt
     9721 tttttcttta ttatagctct tttttgtaat aaatcataat atcaagagaa aaattttcta
     9781 tagaaaggtg gatttttatg agtattcgtt ctccgtgtat ccaaatgagg tttgttgcta
     9841 ctttcgttat ttgatatact aaaaataaac aacgaaagaa ggaataaaat gaagaaaaag
     9901 caactatggt ttgtctctat gtttgcaata ttttttatta ttttaagtgg atgttcaagt
     9961 aataagaaat cggaagctaa agaagataat agtttattgc aaccaggaac tgtttggaaa
    10021 gaagaagtag gtggtcttat tcataattta aaaattatag atgatactac ctgggaatat
    10081 tctgaaagtg tatggcatcc agatcctgta caaataacag ttaagagaca aaaagactat
    10141 gaagggttag aaagatacaa aatagtagat tctgcaggta tcagaaaatt tataaacaaa
    10201 cccgattctt tatttatcgt tgttccttac gagaaagatg gcgtaaaaaa aattgttttt
    10261 cttggatcta gtaaggatga aaagcgaacg aaagaaaaat tgattcatga tggatcacaa
    10321 tcaaataaat acaaattaca aaagactagc gagtgattgc tagtcttttt atttacccta
    10381 aaggaggaat catagtggca aaagcgaaaa cgccatttga aaaagccgcg tctaaaatct
    10441 tagaacgtcg cggtgtatct gaaacacagt ggaaacaaga agtccttgaa aaagcacaat
    10501 ttgacctatt acggggaaaa gacaaggact tagaaggata tatctatcaa cgtgaacgtg
    10561 aaaagcttgt tttcgatgaa attattaaac actaaaagga gcgaacaatt ttatgtttga
    10621 atcaattaca atgaattttt taacacaacc gaaagtagta gctgcggttg atatttttag
    10681 agcattacaa actgccgcaa ctagtttgaa aaataatctg atgatttttg ctgctgttgc
    10741 ggcagtggct tccattgctc ttggaggtat tttcttcttc tttgggcgaa gaggagctga
    10801 aaacgggaag acacacgtag gttatgtatt gttgggggta ggagttattt tagctgccgc
    10861 ttctattgta gtttcaatgt tctcaatgtt tggacaaacc ccacaaggac tataaaaaaa
    10921 acaagtagaa ggagtaaaaa gtacgtggct actttttact ccttttttta tgaaaggaga
    10981 atattcatgt tttttacgat ttcaggaaat gcaaaattgg ataaagagtt aataagaaaa
    11041 gcaacgaaga aagtgggaaa gcgtacttcc tttttaccga aaacaattaa atattctgtc
    11101 attgaaacgg aagaacagct acatgtgtta ggacaagtgt tagaacaaga gaatcaatct
    11161 tctgttaaat tgactctcac gttacatgat aaagaaggaa aaatagaaaa ggctggcaca
    11221 gaatacgttt ttggcccact ggattatcaa atttcagaag gtttttatcc tacattgatg
    11281 gatttaatcc gccttgatgt gtttaaagat acggatgaaa ctatgagtgt ggaagagtct
    11341 tatcgaaacg aaaaactttt acaagtttta gaagaagagt tagagagcga ccaaagaaaa
    11401 gtgaatcaag tgagtgaagt cgaagtaaat gaagaagtac catgggaacg accaatattt
    11461 ccaaagaagg agtcaaaaga aaaagagaca gtctatgttg acgaacaagc tgatattccg
    11521 actcagaaat cgatatttga ggaagagaac gatgtgatgg gtcaattgga ggtagatccc
    11581 tttgaagaaa caatggagga tgaagagacg gacgaagtag aagttgatcc agttgaagct
    11641 aatgaagagt caacatggac tgaaggggat tctcaggaat atattgaacc acttatcagt
    11701 gaagaagtag aaaaaaacga aacagagata caaaaggcaa ctgtatttga ttatattttg
    11761 gatcaatatc cgccagagca tgaatggatg aaagaaaaaa acaaattatt tttaaatgag
    11821 ctttattcga aatatcaatt accccaaacc tatcaagcat ttttagagag taaagaagaa
    11881 ttaatctcac agggtaaagg cacactttta gaacagttag agtctgtcca atcaacaaat
    11941 tggcatgagc ttgcacaaga agatttatta gccacctttg aagaacgtca acaagaaaca
    12001 gatcaagaaa ttaatgagta tgcgacggaa cagtcgtcta attgggagaa agtaaaagag
    12061 gaactaaata aagaagaaca gcgagtgatt gaagaagaag tacaaaaaat caaagagcgg
    12121 tacgaaaaga aacgcgacaa tgcttattcc ttgtgtgtag aaaagatcaa caattttact
    12181 aagacgaatc aagaagcgtt atcgaaagaa aaagaaaact tgttaaaaga acgtattaaa
    12241 gaacgaaaag aagagttcta ccgcggttta cgtacagata agctaaagat tagcaaagaa
    12301 ttaaatgaac aactgaataa gctatatcaa acaacgactg aagttcttat ggaaaaacac
    12361 gaacagatcc agcaagaatt acaagtacaa attccaaaat ggaaaaaaga gtatgaaatt
    12421 caacaacaag aattgaaaga acaagaagaa aaacaacggc aaaaagaaaa agagcgtgaa
    12481 caattagaaa taagaaaacg ggaacaggat cttgcggaac aaaaaatggc gatagaaaaa
    12541 caacggttag agcaagaaaa ggaaaaagaa gcagctgctc gtaaggaacg tcaagaacaa
    12601 atggatacat tgcgtcaagc acagttgaat tatctagcac aacctcaacc agtagctgct
    12661 gttcaaacgc cagtgaacaa ccaagcaaat ttaactcctt caccccagcg ttcctttaac
    12721 ggatggatgg tgggatgtat tgcaagttta agcttgcttc taggtggagg aacagtattt
    12781 gcttttaatc atatcaatca atcttcacag gctgattctg tagccacaac ggcgtcaatc
    12841 aaggaagaag cccaggaaca agcaagaaaa gagtatgaac aacaattaga agaattattg
    12901 aatcgtacca atcagtcaac aacagagtca agcatggaaa atagcaaaga gagtgattct
    12961 agtaaggcta aagaaggatc aagcgattca actgtacaat caaacacttc cgaaagttca
    13021 gaatcaaatg aggtgaaata atgcgtgtta gagaaacaac gaagcgaaaa gacaaacgat
    13081 tcaatttaga aaagaatagg tatctttctc atttctatcg ctatactccc tcaggagaag
    13141 agctaccaga aaaacatgac aaacgcattt cgaaaaagat tttttatagt ggacttgtat
    13201 acctcccttt agagtattta gtaacaagag taccagaagt tttcaaccga ttggaaattg
    13261 ttaagggaag cttacaagga ataaatctgt ttcaattaca ggaaatgcca caggtctttc
    13321 attttgattc agctggtttt tggaatccat ttactcctac aactagtttg ggtacacgtt
    13381 tagcaatttc gacagtttgt gtggttccag ctgtacttat gggattgaaa atggacatgt
    13441 ggtttcgtcc tctagcagat ggacaaaaag gggataatcg attaatgacc ttaaaagaaa
    13501 tccaacagat ttatccgcaa atacctgaaa caaaaactac ttttccaggt tatggaggta
    13561 ttccaatcac acattacaaa aaatattggt acattcaaac agatactgtt aatacgtgta
    13621 ttgttggtac ctctcgatca gggaaaggtc agattgaagt gttagccacc attgacaatt
    13681 tatctcgtgc agaaaaacaa agttcaatgg tcgttaatga tccaaaaaca gaattatatg
    13741 ttggtagtaa agatgaacta gaagctagag ggtatgatgt atatgccttt aatatcaccg
    13801 atccactgca atcaatgagt gataatccat tggcactcat cgtgaaatac tggaaacgag
    13861 gagacataga tactgcaaca cagttaacca atactttcac aaatacgatt tattatgatg
    13921 ccaacgcttc agataacaaa tactttaacg aaaacgcgca gaaagctgtc aatgcgctta
    13981 tttttgcgtt gttagaacaa gctgatcaaa caggagatta ctccaaattg acaccgaata
    14041 atctcgttga gcttctttct gagattggcg gttttaatta ttatccagat ccagacaatg
    14101 aattcaaaca gttgaatgcc ttagatgaat ttatgaatca gttgccacct ggaaacattg
    14161 caaaaaaaca atatggggca acgaaaattg gatctgataa agcgaaggga aatattttat
    14221 caacggcaat cacgggattg aatccattta ctctaacgaa aattgcaaag atgacttctc
    14281 aatcaacgat tgattataaa tcggttgggt ttccaaaata cttagatttg aaattacatg
    14341 aaagtctttt aaatcaacga gtcgtagtag aattttatca taagggaaag aaatttcatg
    14401 aagaacgtgt caaagtaggc tataaaggat tttgtgaaat caattttgat tgtcatttag
    14461 ttgcaggtga tcaagtttgt ttaaaatacg atcatcaagg aaaagaatat gtggcttggt
    14521 atgaattttc tcaaagagta ttgaaagacg aagagggtaa tatcgtttat cggaaaaaac
    14581 gaggagaaac ccatttgcca gaattagaaa aagaagccgt tctaacatta gatgaggaga
    14641 aaagtaactt attagtagag tctattcgga tgcactatac cgataaacca atggctattt
    14701 ttatgcttac acctgattat gatccatcta accatatcat tgttagtatt tttcttagtc
    14761 agttatataa agaattatct acgcaatgtg ttaagactaa aggagataaa tgtcaccgca
    14821 ggattcattt tattttagat gaatttggga atagtgtgac ctgtttagcc taaggataaa
    14881 agaggctagt tccaacgaac attggaataa ctttactatc gagaaatcaa atgacggggt
    14941 aacgccctga agggtttcca ctgatactgc gacatgcgtt agtaggatgt gcatccaaac
    15001 gcgtatgaag ctcgctgaag tcggcagaag ggtaccgtaa ggaaagacac gatacgtatc
    15061 taccacgcaa gtgctctaga ggtagggtat gtacctgata tgtcgggtgt cctaaaaagt
    15121 tcaataaggt gagaatgatt tgattatctg acaaacttgc gaatggaatg tctgaaacga
    15181 ataactcata gaaatgtgag ggggaagggg gatacgtcct ctttccattg actcgaggtt
    15241 gagtaaaaat gtgtttctat gaaaaacctt gcatggttaa aggctatgga gtagtcaaca
    15301 ggcaaaagca agcacctaag ttgaaatcta tggatagata gtaaggaaca ggggaagcat
    15361 ctagcacgga gggagcatac cgaagatgtg tttctaagga aaaaccgaaa tcggttataa
    15421 cttaggtgaa tagatgtgaa agtagagcca cgaccagtga aaatttcgta atgaaattgg
    15481 aggaacaggc tcaagtcgtt atttataata taagaaacct atttaaaatc atggattctc
    15541 gtaagactaa aaaggtcacg aaccagaatg gagcgtgata aaaccatgac taacacaaaa
    15601 ggaattagct taaagaacaa acaactcaga cacgccgaat actacgacat gactgagact
    15661 ttcgatgaat tgcacgcaat gagtgttgca aaatcaaatt ttaaaaatct catgaaattc
    15721 attattgatg acaataatat ctttctagcg tatagaaata tcaaaaagaa taaaggaagt
    15781 gcaacggccg gatacgatgg tgtcactata aaagacattg aaaaattggc atgtggtgac
    15841 tttttaacta ttattaaaag gcgatttaag gattacagac caagaaaagt taaaagagta
    15901 gagatcccga aaccaaacgg aaaacttcga cctttaggaa ttccttcaat atgggacagg
    15961 atagtccagc aatgtattct acaaatttta gaaccaatat gcgaagcaaa atttaataag
    16021 catagctatg gattcagacc aaattgttct acagaaaacg ccatagcaga ttgtcttgct
    16081 agaatcaatc aaagtaagat gaattatgtt gtagatattg atataaaagg attctttgac
    16141 gaagtaaacc ataccaaact tatgcgtcaa atatggacaa tggggattcg agataaacaa
    16201 ctccttgtaa ttattcgtaa gatgttaaag gctccgattg ttatgcgaaa tggcaatgtt
    16261 gtttttccaa ccaagggtac tccacaagga gggattttaa gtccactgct tgcgaatata
    16321 tgcctgaatg aatttgattg gtggatagct aatcaatggg aggaacgaac tgtcaaagag
    16381 ctgaaacacc actatcgaaa aaacggagca tgggataaag gaaatatcta ttcgaaatta
    16441 agaagccaca caagtttaaa agagatgtat ctggtacgct atgcagatga ttttaaaatt
    16501 ttctgccgaa caggagaaga agcccaaagg gctttttatg ccacacaaaa atggctcaga
    16561 gaaagattaa agctcccaat ttcaatggag aaatctaaag ttacgaacct aaataaagct
    16621 gaaagtgaat tcttgggttt ttcaataaga ctccacaaaa agggaaaaaa gaaagtggct
    16681 ttcacgtacg tttgtcgcaa agcagaagaa aacattaaaa ataaactaaa acaacagatt
    16741 aaaaaaattc aatacgctgg agcaaaaaaa aataggcacc gagcaatagg tgactacaac
    16801 atgatggtca tcggtatcca caattactat cggatagctt cacattgtag tgtcaatttc
    16861 actgagattt atagaagtgt taggacagta atagaagcaa gactgaaagc tagaggaatg
    16921 acgaaaaatg gaatctatgg acaagatcat gaaggatacc gtccgtatct aaagtctaag
    16981 caaatgagat atattgaagg ttttccgata cttccaattg gctatattaa aaaaagaact
    17041 ccactcaaca agcggagaaa tctaaataaa tataccaagg aagggagaaa gctcattcat
    17101 caagagcaac aagctataga aagctggaaa atcaagtatc ttgtaaaagg ttttagtaac
    17161 aataagaaat acacggtgga attttctgat aacaaaatat ctaagtttat ttcccaaaaa
    17221 gggaaatgcg ctatcacagg aaacgaactt ttgatggaag aaattgaatg ccatcacatt
    17281 aacccatatc atttgtccaa agacgatagt tacaagaacc ttgtcattat ctctagagaa
    17341 atccatagat taatccatgc aactagtgaa gaaactattc tgaaactgct tcggttactg
    17401 aaactaaatc actatcaaat tagaaaacta aacacgtatc gaaaaaaagc agctaatttt
    17461 ccaatatcaa tcaaaatgaa ttagatagga aacaaattat attgtaatta acgatggaac
    17521 gccgtgtgcg gtgaaagtcg cacgcacggt gtggaccggg ggaaaagacg gagataatat
    17581 caaactctta cctatcggaa ttgccaccaa tggataatat ggaaggaatc atgacggtca
    17641 cagctggccg aaacatgttg tgggatctgt ttattcaatc ttatcagcaa ctttatgcga
    17701 agtatggaga acaaaacgca gcgattatca aagaaaattg tcagacacat atttatatta
    17761 tgtcgacaga tgataatacc atcgaagaat tttctaaaaa agtggggaat aagacggttg
    17821 aacaagagaa cagtaccatc aattcattag gtatgaatac tcatatcaat cgcaatgttg
    17881 attcagatcg tatcttaact cctgaaagaa tttctacttt tttagaaggt gaaacgattg
    17941 tactcgctcc tttaaaacga caagatttga aaggtcataa aatacggccg tatcctattt
    18001 ttaatcatgg ggaaacgaat cttccttatg cttatcaata tattgaagat ttcaatccat
    18061 cgtgggatat caatgatata gacatacatt gtacacatgc ggacttggat cttaaggctt
    18121 tacaaattga ttatgattat ttcctaccag atgaatatgc tagaaatctc tatcatggac
    18181 tgcatgatca attagaagaa aaaaacgaag aaaatatgtt ttataaaatc gttcaatctc
    18241 ttgagaatga gacggctaaa aaattgttaa ttcaatttta cgaagaacaa gcagcagatc
    18301 gacttcaaga atatgttgaa aagttgatca aaaataaaga aatttctgaa atgattggga
    18361 aacggttgtt attggaatct gaaaaggtgg tgaaagaatg atcattggag gaattttttc
    18421 tggcatagcc gactggtggt caggcttatt cggagaaggg gatgcaaacc ccaaaaccgt
    18481 aacgaaagtc ttacaagatt atggtaatta tctgcagtat caagatgtat ttagttggat
    18541 ttggaacact tttctgtggg gattgattaa aggattatat aatctcaata ctttactaga
    18601 aaaaaccatt tatcaaagct ttgatttaaa agacttattg aacgccgctg gggtaaatgc
    18661 actatttcaa agtttgatta gtaaggtatt ggctcttttt tgtgtcgtca cattaattta
    18721 tttaggcttg aaatttttta tgagtaaaca tccaccaaaa gtcaaaaata tcttggttaa
    18781 tttatttatg gccatgatct tgatttttgg tggtagttct ttaatcgatg aagggttaaa
    18841 tatcagtaaa agcttttacg gagacgtcac tgcagcaaat aaagcagata aaacgggatc
    18901 acctgctttt caaattattc aaaataatgt gatcgatgtc agtacgttac tagcaactga
    18961 cccgaataaa gtagaatccc taccaacaga taaaagaaat gctttaacag cgaaaaattt
    19021 taaaacagcg aatatcaatg ggattattac tcctgatcaa gccgctgaga tggcggataa
    19081 cgataaaagt ggattgtcag atttacaaaa ggatcggtta aaggcattac aataccgatt
    19141 ggaactagat gataatggga aagaaatccc tgtggagatt agtgatgaag ggttagcaaa
    19201 atatgtatac acttcgggtt atcgtcgata cattagtaaa ccaggaatta ttttagctgg
    19261 tgaatcttct ttggcggtgg cctatttatt catattattt acgattgtta cttgcattat
    19321 tgagctggta tttaagaaat tctatttagt gattgccgca tcaacggact ttgaaacagg
    19381 acaacggatg aaaagtgcga tagaagatat ttctcagtcc ttcttattat tggcgtttac
    19441 gattttagaa ttacgaatct atacattaat gctttcaggt atcggtgatt tgcatgcggc
    19501 aggaaagatc aatggattct tgtatgttgt tgctttaatt gtgctaacta ttgcgctatt
    19561 caaaggctct caatcggtta cgaagatttt cggtgttgat acgagtctga aaaacggcag
    19621 taattctctt cttagcatgt ttgccttagg aaatattgcg aaaaatgtgg gtagcggagc
    19681 taaaaacctt gcaagtgctg gtaagagtgg tttaagccat ttaaatgata ttcgtaaaaa
    19741 tggatttggc catcgaaaag atgttacccc tgataacggg gaagaacaat cagacgataa
    19801 agctacaact gtacctaata gtaagaaaac agtttccgaa atgttcggga aagcgaaaaa
    19861 tggtgtttct aaagccgcag aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac
    19921 agctgaagat cttacagaaa aagccaaaga caccttagaa gagaaattag acggcacgag
    19981 agctaaagaa ttggctgaag ctgtgaagaa tccatccgaa gcactagaaa aacaaaaaga
    20041 aaaagcttca gaagtcaaag aaggactaaa agatgcgtat ggtgaaggac aagttaatgc
    20101 agcagtaaaa gctaatacaa caaccgaaaa agaaaatcaa gaaggtatag tgattccttc
    20161 agtcaaagat caccaagaag cgttgcactc taatgaaaat tcgattgaag agaaaaattc
    20221 catagaagaa agaaattcag aagaaggaaa aaatggacag ttaccaaaaa ctgatccaga
    20281 acaatatcgg tatagatctg ttaactccga aaatgaaaaa cgcatttcag atttatcaaa
    20341 acgaaatcaa gaagtacaaa cggatgagga ttccatcgtt ctgccaaatg ataatggaat
    20401 cgaagttgga actgaaaaat caacgaaaat tggtttacct aaggcacaaa atttaccaat
    20461 gaaagatagt cgtttgaatc aattcaagga taatgatcaa agttttgttc atcaatcacc
    20521 tgcaggtact gttcaaatga aaccaagtcg tttgcctgaa aatggaaatc tgacaactgt
    20581 ttcttacaat ttatctgaaa gtggtgagac agggaatgcg tccgttcaaa atgttgtgac
    20641 aagagaagct gcaccaaaac ctattcaaaa cgttcataca ggttacaagc cagcaaatgt
    20701 ggcatttact gcaccaacaa aaataaatgt aacaagagaa actaaaaaca ggaacaatga
    20761 attgaaagaa caattattga aggatatcaa atcataaaag gcctacgagt taggcctttt
    20821 ttctatgtat gaaaaggaga aatcatttat ggataaatat attaacatga aaaatcctcc
    20881 aagatttagt ggggaatgga agtttttcgc ctttttcttt ttagatctat taacgcttgg
    20941 tggttgcttt ttttgggcga aaatagtcaa taatcttgtc cacttaccac tcttttttgc
    21001 actcttgaat tatggcttat ggattgcgat agctctagtg attgtttggc gaccctataa
    21061 taacccaggt caacgacaaa tttttgcgat tatcgaatct attttcttta cggataagaa
    21121 ccactatcat gcgttagatc ctaaccgaaa ttatcatggt acgtataagg aggatgaata
    21181 aatgcgagtc aaaggacaaa attcagttga taagaaatcc attttgcagt ttggaaaacc
    21241 agaagaacga tctatcttgg atttatttcc ttataaatcc tgtacagaag agggagtctt
    21301 agttactaaa gaagatcatt ttcaacgtta ttatcgtgta gtttctacag acgtggaagg
    21361 attaaacgaa caagagaaac tagaacgaat gaatcagttg accaccgtca tgcgtacgta
    21421 tgtgccaaca attaagttag ttagtttgac cacagagacg gatctaacgg aacaaatcgt
    21481 gcaaaagcgg caattattag ataagaatcg tatggaacaa tccttaggaa gaaatctaca
    21541 gcgattaaga aaatatgaaa agaaattagt agaggaaatc caggaattga aacgagcaga
    21601 acaagaacgt cctgacttaa gcttcttttt cgtcattgaa ggaaagacaa tcaaggattt
    21661 acaaaccaga aatcgtcagt taatgcgatc tagtggcatt ttagggttaa aaccactcta
    21721 caaaaaagaa ttgatcaaga ttctttatcg aatgaacaat atgaatgatg agtaggaatg
    21781 gagaagagag aatgaaatat ccgttgatat ggttagaaaa tgatgttccg aaggaagaga
    21841 tcactagaaa atttcctcac actttaagcc tgggtaagtt gggaaaaggg caatctttta
    21901 accaagtaaa gaatcaaaca agaagaggtg actgtacatg ttagaagcga ttaaaacagc
    21961 tttttcacca caaaatgatc aaaaagtact tgatccatta aaacaactag gaaatcggga
    22021 aatagaaatt ttaaaatcca aagggtatga tttagatttt gtaaaacaag tgatgccaca
    22081 aggtggatta aatttcgatg aagagtatgt gacctatgga aatggttatt cacgatgtgt
    22141 gacagtctat cactacacaa aaaatcctac tcttttttgg ttggccaatc tcatgaacaa
    22201 tcctcataca gtggccacac tagatgttag cacagatgaa aagcaaaaag tgattgatca
    22261 aatcaaccgt gcattagatg aattaaatga tcgatcagat aatgaacgaa aagcgactga
    22321 ccgaaataaa agtgcggaag aatacctgga actagcggaa tatgcccgaa aattgacttc
    22381 taatggggaa atttctaaac aagtcaaagt gagagtacat gtttacggaa acacgattca
    22441 agaagtagat gaacgagcgc aggaactgat tgaaaatctc aaagggatgg attatcgtgc
    22501 ggtgacttat ctgcataaaa ccaaacgaga ttggcaatcg atgtttacca tgttcgatga
    22561 acaagctgaa tggttcggaa atatcaaagg gcaatcacta ccatcgttaa acattggcgg
    22621 aggtttcccg ttccaccacc aaacgctaaa agatccagga gggacacaat atggggaaac
    22681 catgacaggc ggcccatttg tctggaatgc cacgtataaa aatagtgtac ggttgtctac
    22741 taataccttg cttctaggaa tgatgggagc tggtaaatcg accgttctta aaatgatcgc
    22801 ggaagcccat ttagctgcag gagactttat ttggggcttt gaaaagggaa aagactttat
    22861 tcctttctta aaggaatata acggtattat ggtgcgttta gacggatctg acgggatgat
    22921 caatccttta gagatatttg ccacacggac gtatgatgaa gcatccaggt tatatgacga
    22981 tggatcagtc aaagatttga agatcaatga agcagcgtcc tatcaaacgc acttagacaa
    23041 agtagtttat caggtacagt tagtgagtcc tcagctaaaa ggaacgatga aagcagaatt
    23101 taagacctac ttaaatcgtt tttatgaaga gtatggaaca gtaccacgcg gctttacttc
    23161 ctcaaattcg cgttcaaata ctgatacgca agtcacaggt aaagatccag aagcttatcc
    23221 gacgttcaaa gaatttttag actttttagg acaactggaa ttaccaggtg cttcccaaga
    23281 gaaaaagaat cgaaaagaag aaatggaatc catcgtagaa tccttatgtg agacgtatgg
    23341 catgattttt gatggtcata gtacgattcg acaccttgac caacaacagt tggtcttttt
    23401 tgacattgac tctattacag gcttagatcg aggggtacaa caatgtcaaa tgtacatggc
    23461 tatgacgttg atttggaatc aggcattgat tcaaggaaga agacaacgac gtttaattaa
    23521 agaaaaacaa atggaagaac aagacttcaa atgcttcttg gcgatgattg atgagtgcca
    23581 taatgcgatt aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga
    23641 aatgcgtaaa gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc
    23701 agaaaatatg tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac
    23761 gaagatcttt atgcgtatgg atgaatctat tttacgtcat atagaaggat tagttggtaa
    23821 atcaatgaca aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat
    23881 caattttggc tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa
    23941 attgtttgat ggaggaaaat aatggaaaac caaaatgaaa gtttaagcaa gcagtatgtt
    24001 aagcgtcgtg caaagcgacg cttatttttg tggttgtttg gtacttctgc aggtttgatt
    24061 actattttga ttactgtgtt cgtgaccctt tttctaatac ttgcggcggg ttcgattgat
    24121 aattctgatt cagattcaag tagtggtggc gaagctttta caggagaata ttcggaaggc
    24181 ttaccgattt ataaagaaat caaaggaaga ggcccttttt ctgatgagat tgcgcaatat
    24241 gctgtaggtg cagcagtgaa atataaatta ttaccaagtg tgatcctttc gcaatacggt
    24301 tatgaatcag cgtttggtac gtcggcgtcc gcaagaaatg atctcaacta ttttgggatt
    24361 acttggtttg atggttgtct gtttcctaaa ggaactgcgc gaggaatcgg tggtattgaa
    24421 ggtggctggt acatgaagtt tcccaactca aaagcagcct tctcctatta cggctttatg
    24481 gtagccacac aatccaattt taatgcatgt gtaggaaata aaagccctgg cgcaagctta
    24541 ttgatcttag gacgaggtgg ttatgccgct gcaggaatta ctgaagatag tccgtattat
    24601 acaggttgta tgtccattat cacttctaat aaattaaccg aatatgatga attcgccatc
    24661 aaacattggg gtgaaggtgg caataacaac ggcacgatca ctggtgaatg gacgaatcca
    24721 ttccctggca gcagtttaga taaaagttcc ttcagtggtg gtcaattatt tggaacaaat
    24781 ccaggcggtg aattccgtcc gaatggcttc catgatggat tagactttgg ttcagtcgat
    24841 catccaggaa gtgaaattca cgcggtgcat ggaggaaaag tcgtgtacgt tggcaatcct
    24901 ggtatttcag gtttaggtgc atgtgtgatc gtgattaatt acgacggttt gaatatggta
    24961 tatcaagaat ttgcgaatag tacaggaaat tctcgtgtga aggttggcga tcaagtgaaa
    25021 gttggtcaag taatcgggat acgagataca gcacacttgc atttaggttt cactcgaatg
    25081 gattggcgtc aagcacaagg acacgcattc acagacgatg gtacatggat tgatccatta
    25141 ccatctttga atagcagcaa aaaatagatc aggagaaaaa atatggaaaa taaaacttta
    25201 aaaatcgtct taacagctgc aattagcgtt agtttaatcg gcaatatcgc gttgttttgg
    25261 cagctgaaag aagaacaaga gacaaatacc tttaaaattg aagaagtggc taaaaacgcg
    25321 tctgagaagc tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa gttaaatcaa
    25381 accatcaatg agaaaaacca agaaatcgat cagctgaagc aacaagatgg cacaaaagca
    25441 aaagaggaag taactgaagc acaaagaaag acggcagaag agttcggtca attggccatt
    25501 gataaatcat taagtagaaa tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa
    25561 gtgatcgaca aattaagtcc tgcagacgat gatcaccaac atgatgatta cggaagttac
    25621 tcctttacat taggtgacgt gcaaacttat gctcaaacaa gcagtgtaaa agaggaacaa
    25681 aattttgtcg tatttgttga ttatacaatc ggcaatcctc aatttaaaga tgtcaaacca
    25741 caaaaaataa aaggtggatt aactgtcact gaaaaacaag tagatggtca atggaaagtc
    25801 acagacttta gttatttcac tcgttaggag ggagaaaatg agtaagaaaa aaggagcaag
    25861 acaacgtttc aaaaaacgga aaagttatct tagatggctg ggaatcttta tgattctcgg
    25921 ctttttaatt cttgctagtc cgatttatac gcataagaag gtagacatac cagatgaggt
    25981 aagcacgaac gcattcttaa acacgaagga actgacgatg gtttcaaaag aaaaaggagc
    26041 gaaccagcaa ttagtttttg aatttgcgat cgacgatgaa gatcaaagct tattaaaaga
    26101 actggctaat cttaattatc gcgtagaagt aaagacagct aaaggggatt atcaagcaat
    26161 aaaagccaaa gtgatcaagg tgtctgatga ttattttgta gtgaagttag cacatgtacc
    26221 agaagaatat atcgccatgc gtttaaccat tattcctgaa aaaattgatc caaaagtgga
    26281 tatgcaagaa ccacaagatc tgatttatta cgttcacgaa gataaggtta aagatcaagt
    26341 gaaagatgaa gattatgaac aacatgcgat caattacaaa gtgaaaggat acaagaaaga
    26401 acaaaaagca gcacaaaaac aaattgaatc cttccaagca accattgatt tgaatgaaga
    26461 attagtgaag aaactaaaag atcaattgcc ttatcaagtc gcagacgatc aggaaaatac
    26521 tgaaaataag attaatagtt accagcaaga aattgaaaca tcgaaaacgc aaatgaaaga
    26581 tcaagaagag atcattcaga aacttcaaga aaaaattgat cgtattacaa aagaaaatat
    26641 ttagtagaac aggtggagtg aaagaaatga agcgagcgaa cttggtcggc aaagatactt
    26701 tcgctgagca gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat
    26761 gtcaagaagt gaatttatct aacacaacaa tatctgagtt gaagaatggt agaaagagtt
    26821 tagatac
//
LOCUS       DAZUJO010000030        26165 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000030 DAZUJO010000000
VERSION     DAZUJO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.030"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1097)
                     /gene="queA"
                     /locus_tag="ACG8OL_001389"
     CDS             complement(66..1097)
                     /gene="queA"
                     /locus_tag="ACG8OL_001389"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS4817385.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1290..2465
                     /locus_tag="ACG8OL_001390"
     CDS             1290..2465
                     /locus_tag="ACG8OL_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS4817386.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2469..3110
                     /locus_tag="ACG8OL_001391"
     CDS             2469..3110
                     /locus_tag="ACG8OL_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817387.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3107..4024
                     /locus_tag="ACG8OL_001392"
     CDS             3107..4024
                     /locus_tag="ACG8OL_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4817388.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4021..4692
                     /locus_tag="ACG8OL_001393"
     CDS             4021..4692
                     /locus_tag="ACG8OL_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817389.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5233..5721)
                     /locus_tag="ACG8OL_001394"
     CDS             complement(5233..5721)
                     /locus_tag="ACG8OL_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS4817390.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5922..6188)
                     /locus_tag="ACG8OL_001395"
     CDS             complement(5922..6188)
                     /locus_tag="ACG8OL_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS4817391.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6188..6451)
                     /locus_tag="ACG8OL_001396"
     CDS             complement(6188..6451)
                     /locus_tag="ACG8OL_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4817392.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6972..7112
                     /locus_tag="ACG8OL_001397"
                     /pseudo
     CDS             6972..7112
                     /locus_tag="ACG8OL_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7254..9077)
                     /locus_tag="ACG8OL_001398"
     CDS             complement(7254..9077)
                     /locus_tag="ACG8OL_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4817393.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9675..12380)
                     /locus_tag="ACG8OL_001399"
     CDS             complement(9675..12380)
                     /locus_tag="ACG8OL_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS4817394.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12686..13633)
                     /locus_tag="ACG8OL_001400"
     CDS             complement(12686..13633)
                     /locus_tag="ACG8OL_001400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS4817395.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13823..14092
                     /gene="rpsN"
                     /locus_tag="ACG8OL_001401"
     CDS             13823..14092
                     /gene="rpsN"
                     /locus_tag="ACG8OL_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4817396.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14268..14630)
                     /locus_tag="ACG8OL_001402"
     CDS             complement(14268..14630)
                     /locus_tag="ACG8OL_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS4817397.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14641..15762)
                     /gene="alr"
                     /locus_tag="ACG8OL_001403"
     CDS             complement(14641..15762)
                     /gene="alr"
                     /locus_tag="ACG8OL_001403"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS4817398.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15778..16128)
                     /gene="acpS"
                     /locus_tag="ACG8OL_001404"
     CDS             complement(15778..16128)
                     /gene="acpS"
                     /locus_tag="ACG8OL_001404"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS4817399.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16258..17769)
                     /locus_tag="ACG8OL_001405"
     CDS             complement(16258..17769)
                     /locus_tag="ACG8OL_001405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS4817400.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18091..19443)
                     /gene="murF"
                     /locus_tag="ACG8OL_001406"
     CDS             complement(18091..19443)
                     /gene="murF"
                     /locus_tag="ACG8OL_001406"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS4817401.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19596..20672)
                     /locus_tag="ACG8OL_001407"
     CDS             complement(19596..20672)
                     /locus_tag="ACG8OL_001407"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS4817402.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20868..22613
                     /locus_tag="ACG8OL_001408"
     CDS             20868..22613
                     /locus_tag="ACG8OL_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS4817403.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22893..23330)
                     /locus_tag="ACG8OL_001409"
     CDS             complement(22893..23330)
                     /locus_tag="ACG8OL_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS4817404.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23314..24687)
                     /locus_tag="ACG8OL_001410"
     CDS             complement(23314..24687)
                     /locus_tag="ACG8OL_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817405.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25009..25497)
                     /locus_tag="ACG8OL_001411"
     CDS             complement(25009..25497)
                     /locus_tag="ACG8OL_001411"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4817406.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25519..26121)
                     /locus_tag="ACG8OL_001412"
     CDS             complement(25519..26121)
                     /locus_tag="ACG8OL_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4817407.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7504 a         5561 c         4837 g         8263 t
ORIGIN      
        1 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
       61 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
      121 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
      181 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
      241 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc
      301 gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca
      361 tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta
      421 tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca
      481 tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc
      541 gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt
      601 tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc
      661 aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg
      721 tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc
      781 ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc
      841 gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca
      901 ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt
      961 tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa
     1021 tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa
     1081 tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg
     1141 tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata
     1201 aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct
     1261 tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt
     1321 ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca
     1381 tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa
     1441 tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc
     1501 cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca
     1561 tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac
     1621 ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc
     1681 gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg
     1741 cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc
     1801 gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat
     1861 ttgtaaccca tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg
     1921 aaggccgtgt gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt
     1981 tcgtagaaga gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag
     2041 tcggcgaagt tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag
     2101 caatcaaact gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg
     2161 tattaaaagg atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg
     2221 tcggcgacat tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata
     2281 ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca
     2341 aagtcgtagg tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg
     2401 gagaagatcc cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg
     2461 tctgacctat gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg
     2521 aacatcttta tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg
     2581 gtgtcttact gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct
     2641 tgcagacaat cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta
     2701 aattacctgc gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt
     2761 acattgggat gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga
     2821 ctgagttcca atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg
     2881 gtattcgttt agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg
     2941 ctggtggttt aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt
     3001 tggcaggtac gatccctgta acaattttag cactattggc tgattggctt ctaggtctct
     3061 tggaacaccg gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact
     3121 aaaaatgacc atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct
     3181 tgccagcaat acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca
     3241 gatcttagca agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat
     3301 catcaacaat ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc
     3361 aatttcagcg gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga
     3421 aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca
     3481 aacgtggttc ccgtcctatg gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac
     3541 cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt
     3601 agctggtgta gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga
     3661 aacttatggc ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc
     3721 agtcgaagca ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag
     3781 ttatgatcta gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc
     3841 agttgtagac aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt
     3901 aggtcatacc atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct
     3961 agtggaacca tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa
     4021 gtgaagcaat tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat
     4081 ggctcctata tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc
     4141 tttgcagcag ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca
     4201 aactgggtga ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc
     4261 attttaatca ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat
     4321 tccctcttac cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc
     4381 cttgatgtcg gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta
     4441 ccactctctg tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg
     4501 ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga
     4561 accaatgcaa cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg
     4621 gcgatcatca cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt
     4681 acatcacgct gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa
     4741 ggctttttta gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg
     4801 ggcaaagagt tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc
     4861 aacgggttca tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc
     4921 caatccattt tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc
     4981 tgcttgttct ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc
     5041 cttttccatc agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg
     5101 ccgtatcggt aacttcgatc atcttttata caatcaggaa cttgccatcc tcatccttgg
     5161 ctaaaaacat atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc
     5221 ggtacacgat tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt
     5281 caccagaaga gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt
     5341 ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga
     5401 aagcgccacg actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt
     5461 ggatacccgc tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc
     5521 aaaagaccat gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat
     5581 tgcttttttc aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc
     5641 ctcactttgg cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat
     5701 tttactgatt ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc
     5761 cattcctaaa ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa
     5821 tcatgcaata aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga
     5881 gactctttgt taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag
     5941 gcagaataaa tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt
     6001 ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc
     6061 gtgggcatat atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa
     6121 ttcgtttgct tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag
     6181 ttactcatca aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt
     6241 tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa
     6301 tcgctctcca gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa
     6361 tctcgatttc agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag
     6421 cagtgctagg attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta
     6481 tagcgtactt gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt
     6541 ttcctctttt ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca
     6601 cttcttactc ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca
     6661 tctttagcaa aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag
     6721 gttcctcgtt gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag
     6781 atttgtaacg gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg
     6841 attgcctctt tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg
     6901 ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac
     6961 ttgacgaggc tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg
     7021 aagaagctga aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc
     7081 aagacatcaa aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc
     7141 tgttcccatc tttaatggct tatagagggc acctttagct gagaataaga caaaacagac
     7201 cctctagctt caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc
     7261 ttcaaatggt agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt
     7321 aaccgcaaac ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga
     7381 ggaatcaaca cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca
     7441 taacggataa ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca
     7501 tctgggaaat gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct
     7561 agcgaaacat gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg
     7621 atatttaccc gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt
     7681 agttttgcat cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc
     7741 ttataaaaaa tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac
     7801 cgatagacaa acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca
     7861 atcgatttgc ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga
     7921 gataaagcaa atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag
     7981 ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa
     8041 tagcctagtc gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga
     8101 ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc
     8161 gctgtagcaa attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt
     8221 tgagacaaaa cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg
     8281 atccccacga agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt
     8341 tttgctcttg gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa
     8401 gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc
     8461 gctcctggaa gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca
     8521 acaacgatca gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat
     8581 gcagattccc tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa
     8641 atagcgactt gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct
     8701 gagacagata ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag
     8761 ccagcattct ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa
     8821 gcatggatga tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg
     8881 gcaatgggta gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt
     8941 tgttccgtac catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca
     9001 aatcgggtta atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac
     9061 ctttttaagt aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag
     9121 tcgaaaattt atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa
     9181 ctttaagaaa ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa
     9241 tacagcaaaa atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat
     9301 agaacttgac ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag
     9361 atgtaaggct gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa
     9421 cctcttgata aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa
     9481 aacataagaa gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta
     9541 caacctcttg gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc
     9601 aaaatatgag gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt
     9661 cacaacctct ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc
     9721 attttttctt cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa
     9781 ccgatcatga tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc
     9841 cgtaacaagt ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc
     9901 cctgagactt ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata
     9961 atagcgatcc ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg
    10021 aacgctaagg cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact
    10081 cctagtaaga aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa
    10141 aatgtcagca tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt
    10201 cctaaatagt attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat
    10261 agtgcagtat aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg
    10321 gtttgtagct caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat
    10381 aatttgatga cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct
    10441 ttttggatac cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc
    10501 acagctgttt cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca
    10561 ttttttagtg ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga
    10621 atgatcggta attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta
    10681 tccatatttc cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt
    10741 tctttttgat atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg
    10801 agaagcgaat caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc
    10861 gtatcgatga cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata
    10921 ccagcaatga tgccattgat attattaaca acggcatcaa tttgatctag caattcatca
    10981 atagccttcg tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga
    11041 ttgcttagca agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt
    11101 ccagcacttg tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt
    11161 tgagatattc cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca
    11221 gtcagcttac catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt
    11281 gccacttgtc caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca
    11341 tctttcagct gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct
    11401 aaatttttca catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt
    11461 gcttcatcga ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct
    11521 gctagttgct taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt
    11581 tttgcttgat ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc
    11641 tcatctactt tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt
    11701 tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa
    11761 ttttcatctg ttgataaaat catatcttta actttattga tacttactag gttcgtatcg
    11821 atgtcgtaac ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat
    11881 tcatttgtga tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc
    11941 gcattgattt tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta
    12001 aaactcagca gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg
    12061 gaacggactc cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta
    12121 tttttatgaa gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct
    12181 gctggcttgt ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc
    12241 cataatgctt tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc
    12301 agaagtgttg caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc
    12361 gtatttttga ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac
    12421 catgtatttc atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat
    12481 cacggcaatt gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct
    12541 ttactttatc atgaaataaa agggaaagat aattttatgc caaagttttt atctattata
    12601 cgtatacgca aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt
    12661 ttttcgtttt ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag
    12721 gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat
    12781 tcattgaaat aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg
    12841 cttctgcatc tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt
    12901 gccgcaaatc tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga
    12961 tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct
    13021 gatcagcaaa ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta
    13081 atttttttac cttttcctca tttttactgg atgcactggt taagattcgg attccctttt
    13141 catggagaag ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag
    13201 tatctgtcgg ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg
    13261 tgaccattcc ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg
    13321 atggcaacac gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac
    13381 ctacatgaac agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac
    13441 ctggtacata agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa
    13501 catcataagg attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt
    13561 ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg
    13621 caaatcttcg catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt
    13681 tgacaaaaaa aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt
    13741 tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa
    13801 aaataattgg aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca
    13861 gcaagaaacg attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta
    13921 cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt
    13981 aatcgatgga cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg
    14041 tgaattagca cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc
    14101 tggctgtaat gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac
    14161 gttcgcttct tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca
    14221 gctggtcctt ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca
    14281 ggagtcagtg cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg
    14341 ggtcgatcca aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc
    14401 tccaacagga taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga
    14461 gtcggaagct taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta
    14521 aaatggttac ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat
    14581 cccacgactg gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc
    14641 ttattcttga tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat
    14701 cgtacctaat tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat
    14761 aagcgtcact tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat
    14821 acatacacgc ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat
    14881 ctttcgtggc cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg
    14941 agtgatatat gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat
    15001 caattcagaa accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc
    15061 agagggatta agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc
    15121 atgccataaa gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt
    15181 tttcagcact tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc
    15241 tgctgtagaa aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa
    15301 tgcagctgcc tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc
    15361 tgcttttaga tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc
    15421 tgcttgcaac cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt
    15481 cagtgcgtcg taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct
    15541 caactcgact ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc
    15601 tgcctcagct gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa
    15661 ttcttttcct tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt
    15721 atgtatcact gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta
    15781 gtcttccaaa ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac
    15841 attgccttca tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc
    15901 gacatcttga aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt
    15961 gcaagcgtaa cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag
    16021 tttcatttca tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg
    16081 agtgatccta ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct
    16141 tttcaataaa tttagaacac tctcattcta ccaaattata accattttgc cacaaataag
    16201 tccacaaata acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta
    16261 atcgttattt gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc
    16321 tttttggctg ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc
    16381 gccgtcacga cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt
    16441 tttctgcatt ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc
    16501 ttttgctgtc gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa
    16561 tttatctgct gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg
    16621 ttcaactgca gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt
    16681 cgtcatccgt tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt
    16741 aacgaaagtc acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac
    16801 gtagctttct ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc
    16861 taatccacgt gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga
    16921 acgcaatacg ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta
    16981 accgcgtgct tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac
    17041 gattgttaat tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta
    17101 ttcttttgcg cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat
    17161 cttcacatga tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat
    17221 cgttgctgag aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat
    17281 atcttccaag aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac
    17341 ggttccaagt ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac
    17401 aacgatatgt ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta
    17461 aacggcttgt acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg
    17521 gatagctaat tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc
    17581 gattttttct aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg
    17641 tccaataacg tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc
    17701 ttcttcaaaa ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt
    17761 aaatttcaaa tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc
    17821 cgtcgatttg ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta
    17881 tccattctta gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa
    17941 aagccaaacg ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt
    18001 ttctatggca aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata
    18061 gtttttcgtt ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac
    18121 ttcaaataag cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt
    18181 ttgtttgata gcatcaatca atttattctt ttctttttcc aaatagaaca caggtagttg
    18241 ggggtatttt tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat
    18301 catggcgaat ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc
    18361 tttccctaat tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt
    18421 tttaaaagta tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa
    18481 aatagctgca ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg
    18541 ggcgagccct tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc
    18601 aatcaaagca ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc
    18661 tactacaaaa tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt
    18721 tccaataccg aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg
    18781 ttcatctgcg ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt
    18841 ggctttagcg attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt
    18901 gattgctgca gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc
    18961 catgcccatt tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg
    19021 aagaccaata ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa
    19081 gacagacgcg gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac
    19141 ttctgcgcct actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc
    19201 tgctacagga ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga
    19261 agcagatgct ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc
    19321 aagaggaacg aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat
    19381 ctttgtcagt gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt
    19441 cattgagatc tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg
    19501 catgactctc acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg
    19561 ttacgcaatc actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat
    19621 tgacgctgat ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat
    19681 ttcaagccca tattttccca taagagtggg tacatactga actccgtaaa tcctggcata
    19741 gagtttaatt cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc
    19801 aatccgcttc cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa
    19861 acttcttctg gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct
    19921 tcataatcat agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct
    19981 tcatttccta atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct
    20041 cgagaatcat actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct
    20101 tttgtaatgc cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa
    20161 gaaccttcac attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact
    20221 ggtacataag gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta
    20281 tccatggcac atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct
    20341 aagaagcctt ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg
    20401 gctccttctt ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc
    20461 tgtccacttg ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt
    20521 aatggacctt tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa
    20581 ttataataaa tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg
    20641 ctgcgtccgc catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt
    20701 tcagtgattt actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa
    20761 aaaaacgagc cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt
    20821 gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa
    20881 atccacgcag ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa
    20941 actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca
    21001 ccttcatcaa gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca
    21061 ctcaaacatt caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa
    21121 agacgatcga aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat
    21181 cgccttttgc ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg
    21241 cggtagtcgt taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac
    21301 tatttgaacg agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa
    21361 aaggggtact ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa
    21421 gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca
    21481 agatttatga cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa
    21541 acttgaatca tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa
    21601 acaagcagct gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt
    21661 ttgatgatgg ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa
    21721 caaaagaaga acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg
    21781 gttcctttgc ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca
    21841 actacatcga ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac
    21901 aagccgtttt agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac
    21961 tttgtcagca agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc
    22021 ttgaacagta ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg
    22081 tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga
    22141 aagcatttgg gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg
    22201 aagtcgcttc gccttttgct caacattatt ttctcactca tctggatgta gaagatacat
    22261 tgaaacaaag cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac
    22321 agctccacca tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga
    22381 aaaagcaaga caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta
    22441 tgattttagc tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa
    22501 acgcagaggt cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta
    22561 tttttttggc aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg
    22621 ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca
    22681 aatttttgtg aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa
    22741 aaaatgtgaa aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa
    22801 cacatttatc ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt
    22861 cgttacctac tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga
    22921 cgaaaagaca tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct
    22981 tttcttaact ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca
    23041 aattcttttc cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa
    23101 ccataatctt gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc
    23161 gcactatcag ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc
    23221 gtataatggt ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt
    23281 atgacatctt cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc
    23341 cttcttccat aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca
    23401 ttttgccaag ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat
    23461 caataataga tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa
    23521 taaaaactgg tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg
    23581 atccattgga atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac
    23641 tacgtgccag aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac
    23701 caataaagaa gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt
    23761 caaaatccat ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat
    23821 aaaaatcgaa agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa
    23881 aaggaaattc cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt
    23941 ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc
    24001 ataaatagca agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa
    24061 caataagcca aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt
    24121 gatgtctttg aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac
    24181 gtaaaaaagt gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat
    24241 cgcttgatac aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt
    24301 gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc
    24361 tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa
    24421 tattccaagc aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact
    24481 atctagacca aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa
    24541 cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag
    24601 ataaggaata aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg
    24661 cccaaatcgt ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat
    24721 ctgctcttcg attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt
    24781 aaaaaaaaat acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact
    24841 caagcagaca agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga
    24901 taaagataga aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga
    24961 gtccttctcg acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc
    25021 ttttttcatt ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc
    25081 tgtaatctca aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac
    25141 tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac
    25201 tgaagaagtc aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat
    25261 ttttccattt atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa
    25321 aaagtaggac acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt
    25381 cagcatgtaa tccacttgtt tttgaccaat gatcggcaca aaagcttccg gccatacttc
    25441 ttgtatgact gccatcagct catccagttc attttctgtt acttttattt tttccataca
    25501 cccacgtcct tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat
    25561 catgctatac acacccaccg accatggttt caagaaaatc acaatctgca taaattccct
    25621 aaaagataat ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag
    25681 cagcaaggta acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc
    25741 aaatttctga tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg
    25801 gacaagcaaa aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc
    25861 aaatccattg ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg
    25921 aatgatcact tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg
    25981 ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag
    26041 aatcaacaca aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa
    26101 aactgatcct tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc
    26161 tcctt
//
LOCUS       DAZUJO010000031        25967 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000031 DAZUJO010000000
VERSION     DAZUJO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.031"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(260..712)
                     /locus_tag="ACG8OL_001413"
     CDS             complement(260..712)
                     /locus_tag="ACG8OL_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS4817408.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(725..1135)
                     /locus_tag="ACG8OL_001414"
     CDS             complement(725..1135)
                     /locus_tag="ACG8OL_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4817409.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1410..1622
                     /locus_tag="ACG8OL_001415"
     CDS             1410..1622
                     /locus_tag="ACG8OL_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFS4817410.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1625..1837
                     /locus_tag="ACG8OL_001416"
     CDS             1625..1837
                     /locus_tag="ACG8OL_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817411.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1977..2132
                     /locus_tag="ACG8OL_001417"
     CDS             1977..2132
                     /locus_tag="ACG8OL_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817412.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2129..2353)
                     /locus_tag="ACG8OL_001418"
     CDS             complement(2129..2353)
                     /locus_tag="ACG8OL_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4817413.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2431..2619
                     /locus_tag="ACG8OL_001419"
     CDS             2431..2619
                     /locus_tag="ACG8OL_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817414.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2758..2886
                     /locus_tag="ACG8OL_001420"
     CDS             2758..2886
                     /locus_tag="ACG8OL_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817415.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2945..3280
                     /locus_tag="ACG8OL_001421"
     CDS             2945..3280
                     /locus_tag="ACG8OL_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817416.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3513..4454
                     /locus_tag="ACG8OL_001422"
     CDS             3513..4454
                     /locus_tag="ACG8OL_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFS4817417.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4456..5346
                     /locus_tag="ACG8OL_001423"
     CDS             4456..5346
                     /locus_tag="ACG8OL_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFS4817418.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5389..6189
                     /locus_tag="ACG8OL_001424"
     CDS             5389..6189
                     /locus_tag="ACG8OL_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4817419.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6204..7055
                     /locus_tag="ACG8OL_001425"
     CDS             6204..7055
                     /locus_tag="ACG8OL_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4817420.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7121..7255
                     /locus_tag="ACG8OL_001426"
     CDS             7121..7255
                     /locus_tag="ACG8OL_001426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817421.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7256..7717
                     /locus_tag="ACG8OL_001427"
     CDS             7256..7717
                     /locus_tag="ACG8OL_001427"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4817422.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7743..8195
                     /locus_tag="ACG8OL_001428"
     CDS             7743..8195
                     /locus_tag="ACG8OL_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817423.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8301..8495
                     /locus_tag="ACG8OL_001429"
     CDS             8301..8495
                     /locus_tag="ACG8OL_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817424.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8526..8819
                     /locus_tag="ACG8OL_001430"
     CDS             8526..8819
                     /locus_tag="ACG8OL_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817425.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8750..9289
                     /locus_tag="ACG8OL_001431"
     CDS             8750..9289
                     /locus_tag="ACG8OL_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS4817426.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9364..9831
                     /locus_tag="ACG8OL_001432"
     CDS             9364..9831
                     /locus_tag="ACG8OL_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFS4817427.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10578..10808
                     /locus_tag="ACG8OL_001433"
     CDS             10578..10808
                     /locus_tag="ACG8OL_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817428.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10967..11800
                     /gene="terS"
                     /locus_tag="ACG8OL_001434"
     CDS             10967..11800
                     /gene="terS"
                     /locus_tag="ACG8OL_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFS4817429.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11793..13208
                     /locus_tag="ACG8OL_001435"
     CDS             11793..13208
                     /locus_tag="ACG8OL_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS4817430.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13220..14749
                     /locus_tag="ACG8OL_001436"
     CDS             13220..14749
                     /locus_tag="ACG8OL_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS4817431.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14667..15719
                     /locus_tag="ACG8OL_001437"
     CDS             14667..15719
                     /locus_tag="ACG8OL_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFS4817432.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15721..16038
                     /locus_tag="ACG8OL_001438"
     CDS             15721..16038
                     /locus_tag="ACG8OL_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817433.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16165..16788
                     /locus_tag="ACG8OL_001439"
     CDS             16165..16788
                     /locus_tag="ACG8OL_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFS4817434.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16803..17693
                     /locus_tag="ACG8OL_001440"
     CDS             16803..17693
                     /locus_tag="ACG8OL_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFS4817435.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17716..17931
                     /locus_tag="ACG8OL_001441"
     CDS             17716..17931
                     /locus_tag="ACG8OL_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817436.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17943..18284
                     /locus_tag="ACG8OL_001442"
     CDS             17943..18284
                     /locus_tag="ACG8OL_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817437.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18284..18655
                     /locus_tag="ACG8OL_001443"
     CDS             18284..18655
                     /locus_tag="ACG8OL_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817438.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18648..19043
                     /locus_tag="ACG8OL_001444"
     CDS             18648..19043
                     /locus_tag="ACG8OL_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFS4817439.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19045..19422
                     /locus_tag="ACG8OL_001445"
     CDS             19045..19422
                     /locus_tag="ACG8OL_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFS4817440.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19423..20031
                     /locus_tag="ACG8OL_001446"
     CDS             19423..20031
                     /locus_tag="ACG8OL_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS4817441.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20031..20372
                     /locus_tag="ACG8OL_001447"
     CDS             20031..20372
                     /locus_tag="ACG8OL_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817442.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20614..23403
                     /locus_tag="ACG8OL_001448"
     CDS             20614..23403
                     /locus_tag="ACG8OL_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFS4817443.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23393..24133
                     /locus_tag="ACG8OL_001449"
     CDS             23393..24133
                     /locus_tag="ACG8OL_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS4817444.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24130..>25967
                     /locus_tag="ACG8OL_001450"
     CDS             24130..>25967
                     /locus_tag="ACG8OL_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS4817445.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9368 a         3723 c         5274 g         7602 t
ORIGIN      
        1 ctgatagtgt ttcttcctta cttaatataa atctttctct atcatgtaat ttatttagtt
       61 tttcatcatt tttttgttct aattcataag cttctttttt tattgtgtta gctttcgaca
      121 gtttagtgat tcctataatt aataaaataa gtccaatcaa taataacatt caaaactcct
      181 catttctttg atataattat tgtagagttt actcagaaat gaggaaagag tccatgtgaa
      241 agcatgggct tttttatttt taatgttgta atactaaatg ttcaagatta tgaggaagcc
      301 ctaaatattc aataatttgg aactgactca tatcagataa atcttgttca tctattgttc
      361 tagttaacat ttcaattgca aacatattag cttcagcttc tataccataa atcatacaac
      421 taaggttgtt tgctctaaaa gctgctgtgc aaataccttt atggagtcta acgtgggcta
      481 tttcatgcca taatacaaat cttttcatag aaggtgatag gttttgatta attaatatca
      541 cataacatct tttattgtgt atcgtttgtc ctaatgtttt attccctaaa tcagtttcaa
      601 ttaaatctat atcagaatat ttgattattt tatatggatt aatcgcatca gatccaaact
      661 ctacaaataa tttttctatt tcttctaata tccagtattt gtttaaatgc ataccaaagc
      721 ccacctattg acgatatttt ttaggagtga attttttctt tgctttaact ttggttaagg
      781 caagtgcaag ttgaatggca tcgtataatt tttccttatc ttcatcgtct aaaggttcac
      841 cataataatt tgtttcagca tctgaattca agccttcaag taatttttca gcttgtaaag
      901 caatatcttg tttatctttt tctgttaaat cataataatg gcgtttatca gtacgtccta
      961 aaagataatc agttgataca ttaaagtaat ctgcaacttt ttgtatttta tctccagatg
     1021 gagtactttt attccatcta gacatgcttc catttccgaa atttaacttt ctttcaagtt
     1081 ctgccaacgt cattgaacgt tcttttaata agatttttat acggtctaat agcataggat
     1141 atgcattcct ttcaaaatac cgacaaaaaa agtatttttc tgcaaattat ctatttacaa
     1201 atagagaatt tgcagttata ctagttttgt aagcaagata tttacggcat acaaagacta
     1261 aaagcaataa actaatagtc tccccagaca tcagtaagct cgctttttgt gcgctgtatt
     1321 ttgtatgctt acataatagc atatttgcag tttttgtcaa acttattaga caaaaatatt
     1381 agaaaatatt cgataagggg gtatgaagca tgactttaaa acagcaagta ttaattgctt
     1441 tagtaaagaa aggttggagt caaagagagt tagctcgacg aatgggtata tcaattactt
     1501 atttacgaga tatcttcatc gaaaaaagaa aacctaagga acgtttaaaa caaatcgaag
     1561 aacttttgga tatcaagtta gaagtagatt ctaatgatca gccatcagaa caggaggcgt
     1621 aaaaatggta caacgtttga gcaaagcgga gtttaaagag aaatataagt attctgaaag
     1681 tacttatcaa agaagaatga aggaatttaa aaaatcacgt tttagtgagg ggtataacgc
     1741 agttacttcc aaagaaataa caattgatgt cgatttatat gaacagttta gaacatggcg
     1801 ttctaaaaat cgatttagag ttagagctgt ttgttaatag ctaaaaaatc taaattcacc
     1861 acttctgaaa agaggagttg ctgctctcct ttcaagtttg ccaaagtcca cgttttttca
     1921 ttttggcaac tcctcttctt ggaggtggtg acggcgagag acgaaggagg gatgacatga
     1981 aggaagaatc aaaaataggg attttgaatg agaacaatta cacaagatta attattgaaa
     2041 cagaagaagg gaaaaaagta gcagaagtta cattgaccga tgcaactcct gctgacggtt
     2101 atcgtattcg attaactcct aaatatgatt aaccttttgg tgggtgtgga tcgtttccgt
     2161 gactatcacg gctttgaatt tttccatctc tgccatgaat aaatagctca gatccttgat
     2221 ttttagaaat ctctctagcg atttttactg cttctgattt tgtattagta tgtgcagtcg
     2281 ctttagaatt tccagcacct ttgacattcc aaccaccatt ggaattaggt acaacatgtt
     2341 gattttttcc catagttttc acctccattt ctatagaaat attttatcaa atagaaatgg
     2401 agaaattcaa taaacgaaag gagaagtgat atggcatata ccttgcaaca agaacatcaa
     2461 attctcggtt tgattaaaca acgcaggaaa caattacaag atgaccgtgc agcgcttaga
     2521 aaagccgatg agctatcaga taggcaagct gaactaattg cttctgaact tgaggatttg
     2581 agaatgctag aaataaaaaa tagggagatt agattatgaa gaagacagac acacttttta
     2641 taggattcat tttggggtta ttagtgattg tagcgcacca aagtattatc gggggaagct
     2701 tgttcgcagc attgatggtt ttaatcaatc tgcttgattc aaaagaaagg agcaactatg
     2761 gcacgagaag aagcgctaaa aatcggtaaa gtgattgctg ataatcggtg gggaaatagc
     2821 cgccctatta ttttaagcaa gcaacatatc gaaaagcaaa aagcatggca acaaataaaa
     2881 aagtgactct gccagccagc aaagagtcac atacaaattt ataaaactag gagaattata
     2941 tcacatgaat aagaaaattg aaaatcttat cgaagaattg aaacgtgaat gccaaaagca
     3001 aggtgtttct attatttgta cagcccaaaa agaaggggaa cttaaaagtc tcgtttatgg
     3061 tgagacaaca gaaattttac tttgtcttgc tatgcaagaa gaacatttag atgaaaatct
     3121 cccattgtca gcacatatta tgcgtagaat tgctgtggat gcttacgaac aagcgaaaaa
     3181 tgaagaagaa aatcaacctt caaatcatac ttttgttatt aataacaagg aagatttagc
     3241 tgatgtgatg actcgtattc tcaaggggga atttcaatga tgaatgaaaa aacaattgat
     3301 ctactcatta ttttattttt cacgttagtg attgtagcga aaagttatgt aatctttggc
     3361 ttgttattcg caattaccat gcttgtgctt tgcgtgttag caagcagaaa agaggttaaa
     3421 catgaacgag ttagcaaatc ttgacaacta tttaactgat cctgattatg ctaaaccacc
     3481 atatgaagca ccaattgatg aggaggatga agatgagtaa atctacctta gaaatgagcc
     3541 atcaagaatg gcttgaagac cgtaagaaag gcatcggagg ttcggatgtt ggaacaattt
     3601 taggattgaa taaatggaaa tctccttatc aactatggct agaaaaaaca ggacaagtag
     3661 tacttgaaga atcaggaagt gagcccgctt attggggtaa tgttttagaa gaagtggttg
     3721 ctaaagagtt tcaggaacgt acaggcaaaa aagtacgcag aagaaaccaa gtatttgaac
     3781 atccattaca tccattttta agagcaaata ttgatcggga agtagtggga gaaaatgcca
     3841 ttctggaatg caaaacagcc aatcaatttc tcggtaaaga atgggaaggc gaagaggtac
     3901 cgcttagcta tctctgtcaa gttcaacatt acatgaacgt tctaaacaaa gactattgtt
     3961 acatcgctgt cttaatcggt ggtcaaaaat ttatctggaa gcggattgaa cgagatcaag
     4021 agctgatcga tacaatcact gaacaattag tagagttttg ggaaacaaac gttcttggag
     4081 gtatcgagcc aattattgat ggtagtcaag cgacagctga tttcttgaaa gagaagtatg
     4141 cagatgttga agacgttcaa acgacgttac caattcattt cgatgaacta gttgaacaga
     4201 aaaacgaact caaacggact aagaaagaaa ttgaatcagc tatccgtcaa gtagacaacg
     4261 agattatcag cgagctaggt aaacgtgaag ccagtatcgg tatcactcaa aaaaacatca
     4321 tcagttggaa atttgtccgt acgagacgta tgaactcgaa gaaactagca gagaaatatc
     4381 cagatgtcgc aaatgatgaa gagatttata acgttactga atcaagaagg ctaaccgaaa
     4441 gggagatcaa ataatatggc aacaaatgaa tcgttaaaaa atcaattggc agcaaagcca
     4501 cagaaacaag ttgcaccagg tcaattagga cttaaagctt tgatgaatac accaacaatg
     4561 agaaagaagt tcgaagaggt gcttcatgac aatgctaatg cttttatgtc gaatgttatg
     4621 actcttgtat ctaatgacag ttatcttgca gatagtgaac cgatgtctat catgagtggt
     4681 gcgttaactg ctgcaacatt aaatcttggg ctagataaga atttaggtta tgcatattta
     4741 gttccattca atagtaaaaa caagcaaaca ggaaaatggg aaaagaaagc tcaattcatg
     4801 cttggctata aaggatatat ccaattagcc caacgatcag gtaaatacaa agcattaaat
     4861 gtgatcgaag tttacgaagg agaactaaaa agctggaacc gactgacaga agagtttgag
     4921 tttgatccaa atggtagaac gtctgatgaa gtcattggat atgttggcta tttcgagtta
     4981 ctgaatggat tcaaaaaaac tgtctattgg accaaacaag aaatcgaagc tcatcggatt
     5041 gctaacaata aagatcgcga taagacaaag ttaagtggtg tgtgggcatc tgattacaat
     5101 gcaatggcac gaaaaactgt tttgagaaat cttctttcta aatggggaat cttgtccatc
     5161 gaaatgcaag aagccaccac atcggatgag agagtccaaa gggttcaaga agacggcagc
     5221 attattgctg aaacagaagt tgaagaagat attcctgaaa gaaaagaagc agaggttata
     5281 tctgaagaaa atgaagatgt acaaactgga ttatttgatg catctaatcc gccgttaaac
     5341 aaataatgag ggagctttct cccttgcttt tctagaggag aaatacgaat gaacacagga
     5401 tatgtcaaat tacatcgaaa agtgatgaat tcattcgttt ggaccaatcc ttatatgtat
     5461 aaattatgga acttgtgttt gatgaaagcg agtcatgaga atcgcaagat actttttaat
     5521 ggaaaagaga tacaagtgaa cagcggagaa ttcgtcacag ggcgcgatgc tatcacatct
     5581 gagatgaacg aaggcgtgaa acgtgaacat caagtgaaca gcggttctgt atggagatgg
     5641 ctaaaacaat ttgaaaaaaa cggaatgttg aacatcaaat caactacgaa atacagcgtt
     5701 atatcaataa aaaactggtc tttgtaccaa agcagtgaac aacaaatgaa catcaaacgc
     5761 acaacaagtg aacaacaagt gcacacaaac aagaatgaaa agaattataa gaatgaaaag
     5821 aataataaaa catcatcgtc gcaaccacgc aaaaagcgtg tttacgacac cgactcagtt
     5881 tactacattc tcgcggagga gttattcaaa cagatttgtc agaatcagga aatcaaaaag
     5941 ccgaatctgc aaagttgggc agatgacatt cggaaaatga tcgagatcga caagcggacc
     6001 gaaaaccagg tacggggaat gattgaatgg agtcagcaca atgtgttttg ggcatcgaat
     6061 attttatctg ctaaaaaatt gcgagaaaaa tacgacacaa tggcagcgca agcaaatcgt
     6121 gattataaaa caaaacaaac taaaacgctt gaatacgaga aatttagcac agatgagttg
     6181 cctatttgag aggaggcgta ggcatggaaa ctgttgggga aatcatggaa aagctgatac
     6241 agaaagtgct tgtccaacgt ggcgaatgtc ctgaatgcgg acagcctttg tatggatggc
     6301 gtacgaagaa ccctgatggc tcagaacgtt gtaagccaac ctgtatgcaa tgtgggtata
     6361 aagcattgcg agttcaggaa gacttacaaa cagaacgaat ttacaacgag agcctgaaag
     6421 caagagcaat caattttttc aaaggtggtt ctgttgtgcc taatcaagcg ttgtttgatt
     6481 gcacattgca gaattatcaa attgtcgatc aagaaacaag acaagcggtt gaagtaacca
     6541 aacgctttgt taattcagtc ttgttaggaa atccaagtca ccttgtttta actggaaaac
     6601 aaggaacagg taaaagtcac ctagcgatgg cagcggcttg ggaagtcttg aagcgatcaa
     6661 actacgataa aaaaatctta tttatcgggt tacaggaaat gctggatcaa atcaaatttt
     6721 cgtacaacaa tcctgaactc agaaaaacga ttgagggatc gttgattgca gatatcaaaa
     6781 cagcggattt agtcatcatt gatgatatcg gttcagaact aggaaaagat gcatcagata
     6841 gtcgagcgtt tggcataaac acgctaaatt cgttcttgga tgcacgacag aacctagcaa
     6901 cgattatcac aacgaacttg cttggtgaag aactgaaaaa agcttacggt acgagaacaa
     6961 tatcaagaat gtttgtcaac tctgatggat ttacgatggt attttctcaa acagcagaca
     7021 agcgcataaa accagtcaaa ggagcgatcg catgaatttg actaaacaag aaaaaagaat
     7081 ctgtcgtcag ataatgttgc aaattatagg tgatcgtgac atggacgaat tatctcaaca
     7141 agagagtcga atggttcgga agataagtaa gctaattatt tatcgggata cagaaagatt
     7201 tctcactgtt ttaggtaacg tctacgagaa tccagagtta ttggaggtag agtaattgcc
     7261 aaaaatatta gatgcatgtt gcggtagtag attgttctgg tttgataaga acaattctga
     7321 cgtaacattt atggattgtc gtcagcagta tgaagaatta tcaacaggtc atgtgatcaa
     7381 tgttgatcca gatgtggtcg cagactttcg cgatatgcct tttgaagatg atgtgttcga
     7441 tatggttgta tttgacccgc cacacttaag acatgtcggc gaaaactcat ggcttgccaa
     7501 aaaatatgga aaattagacg aactttggcc agaagacatc aggcaaggtt ttgcagagtg
     7561 tatgcgagtt ttacgacctt caggaacatt aatttttaag tggaacgagg aacagatacc
     7621 actttctgat gtgttagagg ctattggaga acagccattg tttggtaata aacgaagcaa
     7681 aacacattgg cttgtgttta tgaaaaaatt cagctaacgg aggaaacaaa ggaggaaaag
     7741 taatgaagat tgtattaaat aaatgttttg gtggctttgg gttgagccat gaagcaaaaa
     7801 tggaaatttt taaaagaaaa aatattgaag tattcccata catcaataac tttagctatg
     7861 actcagatga tgaatacacg agatacacag gccaaaagct aggttcgatg gattttatct
     7921 actatttcaa aaaagacccg aaaatcgaca aagttacagg aatttatagc gaaattgacc
     7981 gactgtacgg aattgctgat gattccagtt tttcaagtga ttcaaacaga ggtgataagg
     8041 acctcgttgc tgttgtcgaa aaactaggaa gtgaggccag tggaccatat gcaagtctta
     8101 aagtcgttga tatacccgat ggggctgagt gggaaataag tgattatgac ggcgttgaaa
     8161 ctgcacatta tggttttcaa acaggtagta tttagtccac taatgacaac taaagaggag
     8221 gcagaaggat gagtgaagat gaatactttg aacagtagtc tattaacgac agagattttt
     8281 aactggaaag gggtgtagtc atggacgcat cggaagtagt aaataaaatt aatagtctca
     8341 aagaggtttt cggagacgtc gaagtagtga taaacgcaca aggctatgac aacgaatttt
     8401 ttaagcagat taccgacatt agcattcagc aaggaatgga agacgaagac ggaaacttta
     8461 tcgatgaagt agccatttta gtgacttgcg agtaagtaat tgacagctaa agaggaggaa
     8521 gcggaatgaa actaaaagac ggattttact ccagcggcca cggcatcggc ggtttaatgc
     8581 tagatatgcc gacaaagaat cctaaaacac gtaagaaacc aaaattcaaa gtcggtgaca
     8641 tggttcgctg cgaagcagaa ggattcatct atccatttcg tggatatgta gaacacgtct
     8701 ataatcactc agcaatcatt cgcattgaaa acacgatgga atgtgataag tggttagcga
     8761 aaagcaaaga gaatttagca gtggtgagat tggtggacat ggaagttata aaatcttaat
     8821 tttgcttttt tacaactatc ctacaacaat gagcgttatt ccacaactgg aaggagaaga
     8881 ggcaattgtg aacattttag agaatataga tattaagcaa acaaggaaaa atgctagacg
     8941 attactaaaa agatacagaa atttagaacg tttggtcgga ccagtgaaaa tagacttttc
     9001 cgtgatgact gttaccaaaa atttgaaatt cacaattgac agtcaaaacg aagaaatcat
     9061 tgaagcgata agtactcgag attcggttat cgaagcatta acacggctaa gtagaatcca
     9121 tttccaggta ctttattata gttattgttt tcctaataag atgtcaatgt atcaaatagg
     9181 agaaaaatta ggatattccg atagaactat cgagagaatg aaggcagtag ctttggttga
     9241 atttgctgag gcgtataaat caggagaact catttcacgt acaaaataaa aaaagtcgga
     9301 ttcctccgac tatgaataat atttccgaca taagtattat atcataattg ggggaatcag
     9361 aggatggtac ttttcgatgt aaagaaatat gagacaccaa gcgcgaaaga tgttgatatg
     9421 gaacgcacaa aacataatgt cgctgtgttt ctttcagcat atctatcagc tagatgtaga
     9481 gtagggcaac atcgagaacc aaaagtgaca gcatcttatt ccttggttcc accttctaca
     9541 gctgatcata tatttgaagc agaaagaatg ctgatcgata aagaagaagc acaggaagaa
     9601 tttgagtatc tgcataaatt gtttgttcga ggctattcag cgatacagca tccgcataag
     9661 cctgatgtga ctgaaaggcg caagaagata ttctatgatc gttatatcaa tggtctgccc
     9721 atttatgtaa ctgctcaaag gaataatact agcgaagaat cggttaaagt agaatcaaac
     9781 agaattatca tccaatttgc ttcatcgtta gaactggttg ctttcaagta gccagttttt
     9841 acacttttta taccctttta ttaccaattt ggtttccatt ttataccttt tttataccaa
     9901 tcacttacct attcaacgtt gtattatgat agtgtcgaaa aattaggaaa caggatcgac
     9961 aaaataaaat gtaagggagg aaatctccct catcgtttaa ttaagcttcg ataggcagca
    10021 acggaaatat taagaataag gatgtgaatt tcaactcctt ctaaattgtt cttattatct
    10081 atcatccgtt gctgtctatt gtttttttaa ttattcacac gataactaaa ggtaagtgaa
    10141 gagaaatgat tccattaata atttcaattt ttgcgctttg tcttaatgta tatatgattg
    10201 gatttaaaaa tgggcaaaat aaaaaatagt agcagccaag aataatttta tagtgtcact
    10261 gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc aaccatgcaa
    10321 ggttcgattc cttgccagtg actttggcaa ccgaggcatc ggcggtttaa aaatataggg
    10381 gtgcgcaatt tcgtacgcgt tttgtgcatc gtgtaggttg ctattacata actggttagg
    10441 ttagattgag atttgggatt cggtacaaat gaatcgtcaa atgtctcaag cacaggatcg
    10501 gaaacgtccc tgcctgtgca ttacatatta gatcactctt tgagtggtct ttttctttac
    10561 ataaaggagg ctacataatg agaaactact ggtatgtatc gctaactaat gaatatcctc
    10621 gaaccattga tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact
    10681 ctattgtcga aatggggaga gaagcagaac catgtgagat tgatgcgtgc aagctcgttt
    10741 attgcggtca tggtttctat gatgaaccaa acattcaaaa taatattaac aagaatttga
    10801 gggattagaa tgcaagaaat ggcttaaaaa tctccaatca ggacaaatca agttagacaa
    10861 agtttccgat ttaaagatat tgattgaagc tgatttaatg ctaaaagata ttgaaaatta
    10921 gaaaacaaaa ctcaacctaa acagaatgtg aggtggtgtg tattgaatgg caagacaacg
    10981 tgatccaaga cgtgatgaag ccaaaagaat ttggttagaa tccaacggag aaaagcagtt
    11041 aaaggaaatt gcatctgaat taaatgtttc agattctcag gttagaaaat ggaaatcgca
    11101 agacaaatgg agcgctgaat tgattagtaa cgttaccaat ggcaaaagta acgttactaa
    11161 tcaaggtggc gctcctattg gtaatcaaaa tgctaaaggt aacaaaggaa atagccgagc
    11221 ttctccgcca gtgggtaata aaaacgcttt gaaaacaggt gagtatgaaa caatattttt
    11281 tgacacgtta accgataatg agaaggacat ctattctagt ttgaatgatg atccttcttt
    11341 tgctttgtcc gaagaaatac gacttcttaa aataagacaa cttcgaatga tgaaaagaat
    11401 ccaacaagct gaagctggac taaatgatga agaagtcgaa cgattgcagc agctaagaaa
    11461 gattaaaact ccgattgaaa aaaatggtaa aaagctagaa atcaagcgtg aggttatgca
    11521 agatgtgcag attagcagaa aaaaacatcg caaaattgat gatattcttt caattgaaga
    11581 ttcattgact cggattagca accagttagc taaagccatc aagcaaatga atgaacttta
    11641 tatgaatgaa tacagaactg atttaattaa agctcagact gataagatcc aagctgagac
    11701 aaatgaaatt ggcggaaata attcaggtga agaaatagaa gaatggaaac aggcagtttt
    11761 aaatgccgca aacaaacggg cggtgaaaga aaatgaataa tgaatttatt ccttttgctg
    11821 atattggttc tgccattgat tattactatg ataaaccagt agctttttgc caagatattt
    11881 tgcatttgaa tcctgatgaa tggcaagaaa atgttttaaa tgatttagct gaattttcaa
    11941 aggtttctgt tcgttctggt caaggagttg gaaaaacagc attagaagca ggagcaatac
    12001 tttggttctt aacgtgtcga ccctacgcta aagtaatagc aacagctccg acaatgaagc
    12061 aactttacga tgtactttgg gcagaggtag ctaaatggtt aaatgatagc ttgatcaaaa
    12121 acttactgaa gtggacaaag accaaaattt atatggttgg tgattcagag cgttggtttg
    12181 ctacggctag aacagcgact aaaccagaaa gtatgcaagg ttttcacgag gaccatatgt
    12241 tgattgtggt agatgaagct tctggtgtgt ctgatccaat tatggaagct attcttggta
    12301 cgctatcagg ttttgataat aagctgttga tgtgtggaaa ccccaataat attgaaggtg
    12361 tcttttatga ttcccataat tcagaccgtg ataaatacag agttcataaa gtatcaagct
    12421 atgatagtaa acgtacaaac aaagacaata tagaaatgat tcttaaaaaa tatggaaaag
    12481 aaagtgatgt tgctcgtgtc cgtatttttg gagaatttcc gaaaggtgcg ttggattcat
    12541 ttatcagtct tgaaacagtt gaattggcta cagaaaaaca aattagtgat tctttagtca
    12601 ataaaacaaa ggttgctcat attggtgtcg acgtagctcg atatggtgat gattctacga
    12661 ttctctttcc tagaattgct accagggcat tggagtatga gaagtattca aaacgtagca
    12721 ccatggaaac aacaggatat gtcatcaaca tggccaagaa tctaatgagt caatatccga
    12781 gtattgataa agtgatgatt aaagtcgatg acactggtgt cggaggtggt gtaaccgacc
    12841 gcctagaaga acttatagaa gacaaacatt atccttttga ggtgtttgga gtgaataacg
    12901 gttcaacatc agaagacgat ttttacgata atttaggtac tcaactatgg ggaaacatca
    12961 aggaaatgtt agaagaaaat atgacagcaa atcttaacgg agaacagcct gttattgaat
    13021 tgccttctga tagttcgtta atcaaagaat taagtactcg caaattcaaa atgacaagta
    13081 gaagtcgtat acgtttagaa agtaaagatg atatgaaaaa gcgaaatatt ggtagtcccg
    13141 atattgctga cgcactggct ttagcgtttt atgagccacc aagtcactat caatttattc
    13201 aattttagga ggtgagcttt tgattagtac agtattatct ttagagagat ataagaagct
    13261 acgtgttaaa tatgcaacac aaattgaaga tggaatgttt gatccgaatg gttttataga
    13321 agatatgaaa ccattttttg ctgatcgcga aagaaaatat ctagcttata ctagcgaaaa
    13381 gaatgaaata gataatagac caaagcctaa cactgatatt gtaaaagtta ataataaact
    13441 tcatgctggt atgtattcaa ttgtcgtaga ccaagcagtc aatcatttta ctggtatacc
    13501 tatcaaatgg gattacgatg tatcggaaca aaaaagaaca ctcattcaaa gattaaaaga
    13561 taaattctta aaaaatgata tagaacttcc gacagttcca gaagctttta acaaattaac
    13621 aagcaatctt gattccatga gattcgcaat gcttgattct gaaactgcaa cttttcaagg
    13681 agcttgtgga gtagctttcc gattgctaga gcctgtggaa gaagatgatg gttggaagtt
    13741 aagagcaagt aatattgaac cttggagagc agaaagatat ggaaatgccg gaatctatat
    13801 caaagaaaag tatgactctt accagaaaaa attttttcaa gaaatgaaag ttatgacaag
    13861 aaataaaatt ctgacatatg cttgctatgg tgatttgaat tttgttacaa gcggaacgtt
    13921 caaaaaaatt gatgagaccg ataacccttt ggggacgatt accttggcag aatttaaaaa
    13981 taatacgaat cgttattgtg attttgaagt agctgaggaa attggagatg caattgatcg
    14041 tgctttgtct gatcaacaaa acgaaatcga acaatttaag cttgcgtaca tgcttgttac
    14101 tggcacaaca atgagcaaag gcactgctaa agaaatgatg aatcagctag ggattatcaa
    14161 cttaaaagcc cctactgcaa aagctgaata tgtcacaaaa aatttagcaa aagatttcaa
    14221 tgaatatcat atggatctat tgaaaaaaca gttttacacc atttgtaaag caatcgattt
    14281 taacgatgag gtgtttaaat caaacagttc tggagaagct cgcaagtggc aaatcattag
    14341 cctagaagcg aaaacaaata ctaaagagca atattttaga gaaggattga aagaatgcgc
    14401 agaaacaatt gctgcttttc ttaagtttca tgacaaagta gaaattgagc ctgaaaaaat
    14461 tatttttact ttctctagat ctttgccaac tgatttaagt tatttagctg aagctttacc
    14521 taaacttgca ccatacgtat ccaaacgaac tattcagagc caaattccat ttgtaacaga
    14581 tgttgattat gaaaatgaaa tgatggaatt ggaaagtgga agtgcttatc cagatagtga
    14641 atacaatttt ggcggaggtg gcaatagtga cgatagaaac gaagtattgg accaaacgtc
    14701 gagaactgga ggatcaagca aggctcaaac aagaaaatca gacacttaaa aaattaacta
    14761 gtgtatttcc tgaagcactg aaagagatac aagcaaaact attatcacaa gctgacttac
    14821 acaatatcac ttatccagaa atgatggagt tttacagtac aagtaatcag aaaaaatatc
    14881 gtgaatatgt ggaaaaaaat tataagtcat taaaaatgta tgatgcaaaa tacaaagagt
    14941 ttatcgatga attttttcca ccatttgact atgcaaaagt caatcgctta ttacaaatac
    15001 gatcagatgt atttaaaatt cttgcagaat atgcgatgga tgcagatgtg aatcaatatt
    15061 tttctgatcg cttagaggaa attcttcaaa gaacatattc ttctaatgct aatgtttttg
    15121 ttcaactttt aaacgttgat ataccaaatt atttaccaga aaatgaaatt aaacattatt
    15181 tgaactatcc atggtcagga aagacatttt caagaagact ttggggaaat atttcatcgc
    15241 ttgaacagaa actctctaat gctattgtaa aaagtgttgc tagtggagaa ggcgttatac
    15301 acgcattaaa caccatgaga ttagactcag aaatttgtga catgtttaag ttagaagaat
    15361 caaagtataa caaagcgata gaaaatctcg ttcgaacgga gtatgcaaaa tttgcacaag
    15421 atggtattga aaaatcatat ttagaaacag gtattgagga atacaacgta ttgactgcaa
    15481 aagatgagag agtttgccgg atttgtggag gaaaggcaag taagaatccc tataaactga
    15541 aagatgctgt cataggtgaa aatcgagcac ctttccatag tcgttgtaga tgtacggatg
    15601 ttcctaattt accaaaatta ggaaaggata ttgatgaaga atatgaccgt ttatttggcg
    15661 atttattaga tgagtttgca catgattctt ttggaattaa tttgaaacgg aggaagtaga
    15721 atgaaagatt tttttgaagc agtactaaca attaatgtaa atgctgatat tgcagaagcc
    15781 tacaaaacag ctattgagtc tgagaaccat cctaatggct tgagagacca ttggaatggc
    15841 aattatgcct acgtggttat tggcgatcaa actgttaatt atcaagataa tactccagtt
    15901 gataagaata ccgttaattt aacgattcaa ttattatctc attcattacc aaatttaaaa
    15961 gaaacagttg attggtatga aaagatggga tgtattgttg ttagaactga ctacaaagaa
    16021 ggaaagtcta gtaattaggc ttttttattt tgtccgaaat gacgttaaac tagcgcaata
    16081 ctgggcttgg ttgaatggtg gggcgcaact attaaaactc aaagcaatgc ggggcgtgaa
    16141 aacgaatcgt ggggcgaaag gagaatgagc atgaaacaca aatcattaat gccaatgaat
    16201 ttgcaatact ttgccgaagg tgacgatcaa aagttttctt ttgatgactt caaatctttt
    16261 gtggaatcca atgaagaagc tcaaaaattt attcagtcac aatctcaaag tgttgccgac
    16321 aaacaattgg aagcttggaa acaaaataac ttagataaaa ttaaacagga taccatcaag
    16381 gaatatgagg aatctaagaa aaacaagtca cctgagcaaa ttcaactgga aaaactacaa
    16441 gctgaatttg aagcagaaaa agcgttgcgt gtgacaagtg ataataaagc atttgttgca
    16501 gaacaaattg ctggattaga actagatgga gagttaaaag agtctatttc tcaatttatg
    16561 ctaaataatc ttgttagttc ggatacagat ttcactaaga atgctgttga aggttttaca
    16621 agtgttttga atgcaattaa tgagaaacat gcagatgcat taaaagaact acaaatgaag
    16681 tctgcatttg gtggaactca acaatcgaat aaccaggttc agcagaacaa tgaaacatta
    16741 acaaatccag aagaacaatt aggacaaatt cttcaacaat ttaactagga gagtgaaaaa
    16801 ttatgaaaaa aacatcttta aataatttag agtatttgga tatttcacca gcgattaatg
    16861 ctatgcaagt accaaataca ccttttttaa gctatttact tggtgctgga aaaacagagc
    16921 cagcaaactc gacagaaatt aaatggcgtg aatatgatat caacaacgat gattcttctg
    16981 aaaaacttga gggcggagaa tatccagatg ctgaatcagg tcgaacttgg tttaacaact
    17041 atactgaaat ttttagaaaa tcaacctctg tatctggtac attagatgct attaatgtga
    17101 atggagtcgg aaatgaatta actaatcaag tagcactacg tggtatggaa atgaaaattg
    17161 atttgaaccg aaaattgatt actggtgtaa aagctgatga aaatggttct aaaggtcgtc
    17221 gaatgaatgg gattttgaac ttgatcaatt cagcaaataa ggcagaaaca gccactgcgg
    17281 gtgcagtaac aagaaaagat atcgatactt tatttaaatt gatgtatgaa aaaggttata
    17341 tgggagaaaa actatgcttg atttctccag atatgcagga gttaatgact gatgagttag
    17401 atggaaaatc aacaaaaatt gttcagttcg gggaaagagt aacttttgga ttgcaaattg
    17461 gaaatatcgt gtctaattac ggtacaggta ttgctctact agaaccatca ttgccaaaag
    17521 gaacaattgc cgcaatcgat actaattatg tgaaactacg tccattacgt gaatggagag
    17581 cagaagaact tgcaaaaaca acggattcta gacgtatcgg tcttgtaggt gaatactctc
    17641 ttgaatacaa tgcttcaaac tctggggcaa ttttaaattt aaagtctgaa taaaagggag
    17701 ttagtactcc cttttttgat aggaggaatt acagtggtaa aaaaagatga aactaaaaaa
    17761 gatgaagtcg tgaaatatag agtaggtaaa actaaaaatt ttgttgggtt tgttcatcct
    17821 aaaactcgta gatttatcac agcagattca aataacgaat ttatcatttc tatagatgat
    17881 aaagaagcaa ttgcaatttt ggaagatgca attgatgtta atgaaattta ggaagtgatc
    17941 tgatggatga atcgctaaaa acggaaatca ttgagtctac aaaagaagat tttccagatt
    18001 tgagtgaaga acgcataact aatttattag aaataatttt gctagaaatt gaatcataca
    18061 acacttgtaa aaatgatatt tcatgggaaa agttaaaaag cgtgattagt gaagtgttgt
    18121 ataaaataat aaaaaatgaa tcagaaaaaa cagtatcttc agttagacgt ggcgatacaa
    18181 cgattagtta tgcttcaaca acaaatgatg ttagtgagtt acttctaggc tatggtgatt
    18241 tgatacgaag agttattggt tgtggaggat tggagttttt ttaatgaatg aagcagatat
    18301 tttagaaact acttatgaag atagttgtat tattgaaaga ctgacggaca ttgaagattc
    18361 taatacaaat attactattc aagattacaa aaaagtatat gataatccta tttcttgcgc
    18421 tctttcacaa ggtcaaatcg atggactagc agtcatagaa gatggagaga tggtaaatgt
    18481 ttcaactgac acatataaat tatttgttca tcctaagatt aaactaaaaa aaggagatcg
    18541 aataacaata actcaaaagg ccagtggctt aattttttct ctatttgcta ctaagccttt
    18601 ttactatcct agtcattgtg aagtgaattt gataggaagt gagaaaaatg ggtaatctca
    18661 aatatgaatc aaatgcagaa aagatcattg agaattttaa aaatatgact gtaattgctc
    18721 aaaaagaagg aatatccttc gttaacgatt caatgaataa agtcgttagt ttagctaaac
    18781 cattaactcc tgtaaaatca ggtaatttaa ggcgaggata tcgtgtagta aaagctagaa
    18841 aactatcaag cggtcgaatt gttggagaag ttataaacaa tgaacattat tttaaatatg
    18901 tagaagaagg tcataggact aaaaatggtg gctttgtcaa agggaaattc atgttaactc
    18961 gtgcaacgaa tcttgcaaat atgtcttata ttcctcgaag atttaaacaa atggcaataa
    19021 aaatcgttaa gaaaggaaag taacatgaaa gatgaaatca ttgctgcaat cagtagcaaa
    19081 ttaaaagaaa tctatcctga tgggacaata tatcttgatt cagttatgca gtcaactaaa
    19141 gatttttatt tcgttttatc agtaatggaa tctggaactg aaaatgtagg aattgatatt
    19201 caaaatgttt ctttcttaat tgatattgca ttgattgata ataaccctaa tagaaattta
    19261 attaatgaat tagtctcacg ttgtgggact ttttttaata cgattacaat cgatgagcaa
    19321 atattatttc caaaagccta tttacctgat gaagcagatg gtgttcaaca tattcgtttt
    19381 acattagaat ttccacaata tattgaatgg agtgaaagat aaatgggaga aaaaagaagt
    19441 aaaaccggaa ttatttctgt cgaaaaacct acttggtttc ctttggaaga tgagacaggg
    19501 aattttccgg tatatggtac tgcgttgcca atgggaacag cagtaagtat caaaccaaca
    19561 gctaattatg aaacaacaca agattatggg gattcagtgg ttcaagatca atttacagct
    19621 tttggtggtg cagaagttga actggaagca aatggatata ctcataaagt tttatctaca
    19681 attactggag ggaaaattgt taaaggtggt gccttgcgtt ctggagaaga tattgcgcaa
    19741 gatggtgcgt ttgcatatag acgtaaaaaa tcgaatggga aataccgtta tacggtcttt
    19801 tataaaggac agtttgctct agattcagat gaaacatcaa cgattgaagg aagtaaagta
    19861 agttttactc atccaacttg gaagggttca tttgtagatg taccaggact tggatatatg
    19921 tattcagttg atgaagatga tgaaggcgtg gataaagcaa tgatcggaaa ttggtttaca
    19981 aaagtagcaa ttccaattga agaagcagaa ctttcaggag gtacagaata atgtcaaaat
    20041 atcaaacaac gattaaatta acgaaaaaag acgaagaagg taaatatgag caagtacaat
    20101 ttaaatctgc tgaattttta ccaggaacag ttgtagaaga tgctgcagga gttatggaag
    20161 aaatgcaaac agcgactgat aagcaatcag ttaaaaaagc tttaagtcgt gcttattcat
    20221 ttattgcaga tactttattt gagggacaat ttactggtga agattattgc aaaggaattg
    20281 atgctcgtga gattgcctca ttgacaggaa aattattgaa gtctgttact gcaggttttg
    20341 atgaaactta tacagagacg aaaaaaaagt aagtgaggcg ctcaagtcac cttcatttaa
    20401 gtattcgatt acttaccgag aattagatat aaagacgcaa ttactcgaag caggttggac
    20461 gttaccagaa attgaacgta ctgacttgga tgagttaatg cgtctttttg cttttagaga
    20521 tgcggtaaaa gagcatgaag atgttgagta ctatgataat ttcactcaat tttaggaggt
    20581 gatactttga ataacgatga cctaattctg aagatgatac tagatgaatc tggttttact
    20641 gccggtatga ataatgctgt caaaaagttg aactcttttg atgaaacaat cgaaagaaaa
    20701 agtagaaata gtggcagttc attaggtagt atttggaaaa tatttgctgg aagttttcta
    20761 gctagcggag taactagaat cgtaggagct ggctttgatc taatcaaggg ttccataagt
    20821 ggagcggttg atcgagtaga tacgatgaat aacgctctac gaaatttcca aaatatggga
    20881 tttagtaatt cagaaattat gaagaatatc ggaaagaatg ggcttttatc tcaaggtatt
    20941 caaggacttc ctactgcttt gaatgatgcg ataagtcatg ttcaacttct tgcttcttct
    21001 acaggtgatt taactcgttc aactcaaata tttaaagctt taaatgatgg aattcttggt
    21061 ttcggtggtt caactgatca agttaatgaa gctgttattc aattatctca aagtttctca
    21121 aacggaaaag tagacgcaca aacttggaac tcaatgatca acgctcaact tggacctact
    21181 ctttctgcta ttgctaaaaa gatgggaatt acaatgggag atctgaaaga aggtttatct
    21241 caaggtaaga tttctgttga agaattccaa aatcaattaa tagaaatgga taccaaaggt
    21301 ggcggaggac ttaaatcatt aagtcaaatc gctaaagatt ctactaaggg aattaaaacc
    21361 tctatacaaa atgcaaaaac agctgttaca cgtggtgttg gtgaggtaat agaaggatta
    21421 aataaagcat tagtagattc agatttaggt ggatttaagg gaattattga taaagtgggc
    21481 agttcaatgg aatcgttctt aaaagtaatt gctgcaaata ttcctacagc agtatcattt
    21541 ttaagtaatt tatttaacga aattcaaaaa tttggttctg ctttgaaatt catgatgccc
    21601 tttcttgttc ctgcagctac cgcttttgga gcgttcatgt ttcaacttaa aggtatacca
    21661 gcaataataa aaagcttcaa taattttaag aatgccataa ttggcgttgg aaattcatta
    21721 aagattatgg gggcaatagc tgctgctaat ccatttgttt taattattgg tgcagtcgtt
    21781 ggtgcaatag ctgttttcgg ttactttatg gcaaccaatg aagagcttag aaacaaggtt
    21841 atatctattt ggaatgatgt gaaagattcc gtaattggtg tattaaaaaa tataaaggac
    21901 tggggaattg atacttggaa ttctgctaaa gaaatggcat caaatgcagt tgagggtgtc
    21961 aaagatgctt ggtcaggaac aaaggaatgg ttttctaaca cctggaaagg tattaaggat
    22021 ggggccacag gtttatttga taaaacagta gagacttcaa gaaatgcagt cgatagtgta
    22081 aaaaatgcat ggtctaatat gaagaaatgg ttttctgata cttggcaaag tataaaagat
    22141 tcagccagag aaaaatggaa tggaatcaaa ggttctatta tgtcagttgc tggtccattg
    22201 attacaggta taaagaatgc atttttacat gttacttttt atctagagac cttatggaat
    22261 aatttagttg agattggtaa aaacgtcttt gagattttaa aaaatgttat acttgcgccg
    22321 gttttgttta ttacctccat gatttctggt ggatgggaag aaacaaaaaa taatatgatt
    22381 gctgtttgga ataatattaa agaaagtgcc ataaatattt gggaatctat aaagaatata
    22441 tttgtaagtt attttacaaa tatttatttt gctgcactca atatttggac agggttcaag
    22501 cttacattga taaatatttg gaatgaagtg gtaaatcaag ctaaatcgat ttggttaaac
    22561 ataaagtatt tttttattaa tctttggatt gatataaaat attttgcaat tcagaaatgg
    22621 attgaattga agttttctat tattcaaact tggatcgatt taaaatataa tgccattact
    22681 acttggaata atattaaaca gtttttcaaa gatacttgga aaaatattaa agatacggca
    22741 tacaatacgt ggatttctat aaaaaatacc atgattaata catggaataa cattaaggac
    22801 tctttctgga atattgttac tggaattgtt aattctgctg aaaatgcatg gacaaatcta
    22861 aaaaatggtg tttcaaatgc tattaatcgg gtaaaagaaa tctttaattc gttaagggaa
    22921 gtcaatttat ttgaaattgg caagaacatc attgatggac ttataaatgg tgtaaaagaa
    22981 aagtggaatg ctttgaaaga tactatcaaa ggtattgcag gtaatatcaa aaattggatt
    23041 aaaggagctt tagatattca ttctccatca agatggatgc gtgatatggt tggtaaaaat
    23101 attgttcaag gtattatcgt tggtattgat aaagagcaaa gtaagttaga tcaaaccatg
    23161 acaaatttag taaaaacacc gtctgttcaa cctgtagtaa atggaataag tcacctacca
    23221 attacacaaa caaagcaaaa tatatctgct agtgaaacaa aagaaattca tttacattta
    23281 aatgtttatg gagatttacc tgattcaatg gttcggaaga ttaccacaaa aattaaaaca
    23341 gaattgacaa gacaaatgaa acgagatgct ggtgcagtag gaggaacatt atatgcaact
    23401 taagagagga caatttttta ttaatcaaca ttattcttct gaatttaatg tgtatattca
    23461 aaacaggcct gcttctgttt cagctagtcg agtaattgaa ttgagagagc gagaaggaaa
    23521 tgattctatc attattgata aaggctatta caaaaatgtg actagaaaga ttgagtgtta
    23581 ctataaagca ccttcaattg atatggtgca ggagtgggaa gatcgaatca ctgaatggtt
    23641 agatatgtcg tcttatagtg attttattct ttactatgat gaacaatata tttatcaagc
    23701 tgtagtaata gaaggacctg aatttaaagg aacaagaaaa acaggaaata ttgtcccttt
    23761 tgaatttaca gtgagtattc gaccatttaa ggaaaattac aatggtagat ttactattcg
    23821 acaaacagaa acttttgaaa tttataaccc agagaagtat tcttcaaaac cgcttattaa
    23881 attgagtggt tctggagatg cttcttttta tattaataag gataaatatg atttgaaatc
    23941 gttggacaga gaattattta tagattctaa attagaagag gcttatcgaa aattagatgg
    24001 taatttagaa catcaggatc aggtcacctt atttttagat tttccatttt tatatcctgg
    24061 aaaaaatgaa attaaatgga cgaaaaatat tcattcattt gaaataatgc ctaggtggtg
    24121 gagaaaagta tgaaaccaag aatatatagt cctactgaaa cagatttctc aacgaatggc
    24181 ttaggaattt taaaggattg tacaagatgt ggaatatatg aagtagctaa tggaaaatat
    24241 gaattagagt tggattatcc tttaggaact agatttgatg aatattttga aaatgactat
    24301 caaataaaag caaagccaaa cgatcaagaa gagtatcata tcttttttat tgatgataaa
    24361 gatatagata cttttttaaa tacagtaact atttatgctc agagtcgtac aaatcgactt
    24421 ggaagacggg tagtcactct tgtggaagtg gactctaaaa ctggtcaaga agcgatatca
    24481 attattgaaa ctaaaatgga taaaaaatct gacatacgac tttattctga tattacggct
    24541 gtttctagca caatctttga agcgagaaat gttttaaatt gtattgctgg tgaacaagga
    24601 tcattgcttc aatattgggg cggagaaatt aaacgtgaac catttaagct ttctttgtta
    24661 aagcgaagag gtcgcaataa tgttggaacg attcggtatg gaaaagatat gtctggtttg
    24721 aaggtcaaat tagattggac aggtataaaa acaagaatta ttccatatgc tgatcctcaa
    24781 agtgaagtag gtacgactaa tcgaatttat ggttcgccag tagatagtgc ctatattaat
    24841 aactatcctg atgtctatac agaacatgtt cagtttacag aagaacaagg agtaaaagac
    24901 attaagagct taaataaaat agctaaaaat tactttaaaa cgatcaatcc tggctgtgat
    24961 aagcctaaag tttctattac ggttgaattt gataagttga ctgatagtga agaagcgaaa
    25021 gaatttgcga agattagaaa ttatggtctg tttgatacat ttaaaatata tcataaaaaa
    25081 tataatattt atcttgaatc aaaggttagt ggactacaat acgattcttt atctgaaaaa
    25141 actttgaaat tagaagcagg agacattcaa gttgcttttt atcagcaaca agctgtaact
    25201 attcaggata aattaaaaga ttatgcaacg aataattata tgagtgattt taatgattat
    25261 gtttcttcaa tgattacagg tcaaggcagt gcaggaggat atgtagtttt atggccaaaa
    25321 gaaaaacctt ccaacatctt tattatggat aatccagatt tagaaaaggc aagagaagta
    25381 ctaagaatga ataaaaatgg gattgctttt tctaagaaag gatggcgggg gccttttaat
    25441 tcagcttgga cattagatag tatatttaat gccaatttta tacaaactgg attaatcaaa
    25501 gcagatatct ttcaaaattc ttttaacaag actggagatg tattgaaatt agttaatgga
    25561 ctactccaaa tttggaacaa caaaaagaaa ataatggagt taactaaaaa aggaatggaa
    25621 ttttggaatt ctaagggttc aattggaacg attggaacaa ctgattctgt tggtaatcct
    25681 tttcctgggg cttctactcc aacccctatt gaagataatt ctttagttat tcgtacaaat
    25741 ggagacggca aatatatttt gatttctcct aaagtaggaa aaggattagt tttattagga
    25801 aatggtaaag caatttattt tggagactta gatgtacaag gaaaacttac agttaaagga
    25861 aaagaaataa ttcctggaca aggtggtggt ccatcgggag gtggagaatc tactggtggg
    25921 tatcctagtg aattaaaaac agatgcagaa aagagagctt ggagaat
//
LOCUS       DAZUJO010000032        25682 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000032 DAZUJO010000000
VERSION     DAZUJO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.032"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..272)
                     /locus_tag="ACG8OL_001451"
     CDS             complement(75..272)
                     /locus_tag="ACG8OL_001451"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS4817446.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(418..1296)
                     /locus_tag="ACG8OL_001452"
     CDS             complement(418..1296)
                     /locus_tag="ACG8OL_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4817447.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1335..2453)
                     /locus_tag="ACG8OL_001453"
     CDS             complement(1335..2453)
                     /locus_tag="ACG8OL_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4817448.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2450..4549)
                     /locus_tag="ACG8OL_001454"
     CDS             complement(2450..4549)
                     /locus_tag="ACG8OL_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS4817449.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4600..5421)
                     /locus_tag="ACG8OL_001455"
     CDS             complement(4600..5421)
                     /locus_tag="ACG8OL_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4817450.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5418..6227)
                     /locus_tag="ACG8OL_001456"
     CDS             complement(5418..6227)
                     /locus_tag="ACG8OL_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4817451.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6248..6742)
                     /locus_tag="ACG8OL_001457"
     CDS             complement(6248..6742)
                     /locus_tag="ACG8OL_001457"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4817452.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6754..7254)
                     /locus_tag="ACG8OL_001458"
     CDS             complement(6754..7254)
                     /locus_tag="ACG8OL_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4817453.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7319..10015)
                     /locus_tag="ACG8OL_001459"
     CDS             complement(7319..10015)
                     /locus_tag="ACG8OL_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4817454.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10443..11516)
                     /locus_tag="ACG8OL_001460"
     CDS             complement(10443..11516)
                     /locus_tag="ACG8OL_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4817455.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11513..12340)
                     /locus_tag="ACG8OL_001461"
     CDS             complement(11513..12340)
                     /locus_tag="ACG8OL_001461"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817456.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12341..13147)
                     /locus_tag="ACG8OL_001462"
     CDS             complement(12341..13147)
                     /locus_tag="ACG8OL_001462"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817457.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13159..14244)
                     /locus_tag="ACG8OL_001463"
     CDS             complement(13159..14244)
                     /locus_tag="ACG8OL_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817458.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14261..14803)
                     /locus_tag="ACG8OL_001464"
     CDS             complement(14261..14803)
                     /locus_tag="ACG8OL_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS4817459.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15061..16050
                     /locus_tag="ACG8OL_001465"
     CDS             15061..16050
                     /locus_tag="ACG8OL_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS4817460.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16047..16784
                     /locus_tag="ACG8OL_001466"
     CDS             16047..16784
                     /locus_tag="ACG8OL_001466"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS4817461.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16781..17332
                     /gene="coaC"
                     /locus_tag="ACG8OL_001467"
     CDS             16781..17332
                     /gene="coaC"
                     /locus_tag="ACG8OL_001467"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS4817462.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17348..17980
                     /locus_tag="ACG8OL_001468"
     CDS             17348..17980
                     /locus_tag="ACG8OL_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4817463.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18065..18712
                     /locus_tag="ACG8OL_001469"
     CDS             18065..18712
                     /locus_tag="ACG8OL_001469"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS4817464.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18717..19700
                     /locus_tag="ACG8OL_001470"
     CDS             18717..19700
                     /locus_tag="ACG8OL_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS4817465.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20103..21617)
                     /locus_tag="ACG8OL_001471"
     CDS             complement(20103..21617)
                     /locus_tag="ACG8OL_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS4817466.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21755..22561
                     /locus_tag="ACG8OL_001472"
     CDS             21755..22561
                     /locus_tag="ACG8OL_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817467.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(22736..23305)
                     /locus_tag="ACG8OL_001473"
     CDS             complement(22736..23305)
                     /locus_tag="ACG8OL_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4817468.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(23388..25447)
                     /locus_tag="ACG8OL_001474"
                     /pseudo
     CDS             complement(23388..25447)
                     /locus_tag="ACG8OL_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
BASE COUNT         7665 a         5427 c         4460 g         8130 t
ORIGIN      
        1 ttcttgctat ctttttataa tcacaactct ctataataga attacgattt attttatacg
       61 gctgaaaaaa tctatcacca aaaaaattca aacattaagc caaggattat gaagtcaaca
      121 tttcgaaaaa tgaacataca ttggtaagca actataacta ttaccacaga cataaagtaa
      181 cacaatatat tactatttaa gtgtacgcca ccagtgaata tcctcaaggt atacagcgca
      241 caacaaaaag aaaaaaggaa acgcagaagc atttcctttc agtgatttaa tgaaaacaac
      301 acgatttgac ttagagcctt tttatttgaa ttcattttcg ctttatccaa cttcatttga
      361 tcatttgatt actcggttaa atacttttga catgagtatc aatgaaacat tcattaatta
      421 tggttgctta tttcttaccg cttttctggc caatgctaat ccgccgatta ttccggcatt
      481 atcacctaat tcgggcgtaa taatgtagct atcaattggt ggtatttcaa catatccatt
      541 tataagctca acaaatttat cctgaacttt tttcttcatg tgttcttgtt tcattacacc
      601 accgccaaaa ataattctat ctggcgaaaa catcagtgtt gtattgtatg cacattgggc
      661 aaggtaaaag gcttcaatct gccaaaacga atcgtcagcc aataaattct ggccttttac
      721 acccaaccgt ttttcaatcg ctggtcctgc tgccatcccc tctaaacaat cttggtggaa
      781 gggacagttt cctgaaaaat tgtcctttgg atgtcgcttg atcaacatat gccccatttc
      841 cggatgactg aacccttcca cgaaagtccc attgaacaag gcaccacctc cgatacctgt
      901 tcccacagtg aagtatgcaa cacctgctaa ccctttgcca gaaccagcaa catattcacc
      961 atagcaagcc gcattgacat ctgttgtcca ttcaatcgga atattgaaat attgctttag
     1021 cgttcctaca aaattgaaat tttgccaagc aagttttggt gttgatgtga tatgaccgaa
     1081 actcgttgat ttgcattgaa tatcaattgg accaaagcag ccgaccccga tgcctgccaa
     1141 ttgttcctta aaaggtttaa aaaaatcaat gacctgcttc attgtttcaa ctggagttgt
     1201 tgtgggaaaa ctgactcttt ccaaaatcgt taattcctca gtaccgactg cacagacaaa
     1261 tttagtccct ccagcttcaa ttgccccata tatcatcata ggttcctccc ttcgaatatg
     1321 tgaaaaacac ttcattaacg cggcaagcgt tctcgatcag tgaaatgacg tttagtattt
     1381 cctgaagctt ctgttgaata ccagtaagcg acactagcaa catcgtcttg ccgctcaaat
     1441 aagcgaacat catcgttgcc gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag
     1501 atggggtctt gaagatgcca acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag
     1561 gcatgaacgg cattcaattt tcccgtttca aaacgatctc ttgtggcatc tctatttgat
     1621 tggtaaggat accctaaaaa ggctgttgaa tagtttttcg catgtggacg tccgaactca
     1681 tcgtactcat gaaaggccca cgccccgcca aaataatctt ctgaacctgt tgaggttacg
     1741 gtcggatact cactatcccc atcaatgtag aatttaaact cgccttcgcc ccaccaataa
     1801 cgttctaggc aggtaagcgc aaggtaagta cccacgtaat agccttgacc ctttaatcga
     1861 tcaattaact tataatcctc ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga
     1921 aaatataggc tattttcttc caattcgtcc accaaagcgt aatttatcgt ataaaaaaat
     1981 gaggacatat ccttcggatg taaattctcg atcgtaattt tagcggatgt ttggaaaggc
     2041 atttcgaaat aggaattaaa gccccctgta ggattaacca ctatcggcaa cgagttcacc
     2101 tcgcaacgct ctccaaaccc attacaaaag aaatccccta aaggactttc aacagcaggg
     2161 ctatccgacc catcccaata aattcttaag attaaatcac gtaaaacaaa actgccaaat
     2221 tctgttcgat cacgaatcgt catccacata tgacgaatta ttcccgttcc agtaatatcc
     2281 gcaatcacag tttcttcacc agaagtcagc ggaattgcgc cacgaccttt gcggcttggt
     2341 cctagctcgc ttggcaacat cgcagcttta cctttctcgc ctgttggatt ttcgggagtg
     2401 attgctcgac tctggatttt tttaaattgg gtaatatttt ttagtatact catctttttc
     2461 tcctttgaat gtgaatcgtt tttccctttt ctacgtgaag aatttttttc gtatcactta
     2521 ttttcaatgg cttttctgac aaatttgaaa tactcaactc ggattgcgta cattcaatcg
     2581 aaatgaaggc ttgaccgaac ttcaaattct tccaagaaaa cgttaaatta tcaacaggat
     2641 caatttgcaa tactcctttt ttgaatttca atccgaaata ttgactgatc attaggctga
     2701 caaaaagacc cgatgcccag ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt
     2761 gttgagcctc taagcgatac ggccaccacc accacgaacc gtctaaatct gccaaacaaa
     2821 ttagttcttc taatcgggtt acgaaagctt gagggttgtg cagctcgctc attaaaacag
     2881 tgataaagcc aggaaaagtg gcgccggatt ctaatcccca agtgatgcct ttgatttcct
     2941 cgctataggt tggattgaaa gaactagcag aaaaggccat ggtgttctga tataaatgat
     3001 ctttattatc taaaaattga taaaaaggca tcaacgtagt gtctgattct tctccatcat
     3061 gcatcaataa attaattttt ccatccacaa tcacatcact taaaaagatt aacccttgct
     3121 caagaatcgg tttttgatag tgcttcactg aataggttgt tttctcctca tcaccgattc
     3181 cctccaaaaa ttgctcacca aaaggaccat cgatagtcat ttgtttacaa attgattcct
     3241 ttagtttaca ggcaatgttt ttgtatcgct tgctaagact cagttgttct agggcttcaa
     3301 aaatttctgc aagtccaata caggccttcc aaagacaaag attgctgccg gtatgatact
     3361 ttccaagtgc aaaggcgtct gataaccaaa cagatggaaa aagcattggt tcccctgacc
     3421 gacgttgagc taatatgtcc tcaataattc ttgaagcatt cagtagaacc tgcggacacc
     3481 gctcaaagaa ttttgtatcc ccagtatgtt caaaataaag actagccagt aacgcactgg
     3541 ataacgaatt actcagagaa tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa
     3601 cgccatattg atcaaaccat agaaccgttt tttgacataa ctccggttca aacatggtga
     3661 atggcaatga tgcgtaatac atatccttat tccaaattcg attagtagca ggatagctgc
     3721 cccaattgct gccgacgacc tgtccctgtc gattcatacc gaaactagaa aagctttgat
     3781 aaagtgcacg acgatagaga tggacaactc tatcgtttgg cattgacagc tgcccaaata
     3841 gctgagcaaa atagttcaat gtttccttca gccatttttc gatatcagat tcctgaaaag
     3901 tcattacttc ttcaaaagca tttgggtcca caaaagcaat ggcgaagctt gccacttcct
     3961 gttgtgcgat cgtgtcctta tttccttttt gagaaatcaa cacattttgc tgatccgaat
     4021 acttctcttg atacgaaggc acatctttca ctgaaacttg aaggctttcg tgtgtttcat
     4081 tcacaatgta aacctgttga acaagcatac ttaaacgctt attagccagt atgggagcaa
     4141 aggatactac aatgacctta aacgtcgaat aatgatgaat atatctaggc aaacagtctt
     4201 gaatcacatc gacttcaata cgatgatccg attctgacaa atggtagaca atcccgttta
     4261 cttcaacctt taaggataac gaattggttt gagagagctg tttattgacc caaacacctg
     4321 gtttttcctc agttagtaaa ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa
     4381 catcaaggtt tgctaatgtc ttttgattac ttacatcgaa ggtcaatccc tttacatcag
     4441 gaatattccg atagtagctg tattcgtttg aagtagtcac atttccgtcg tttaactcag
     4501 cattaaaaaa tgggtgtttg ggtgaataaa ttacttcatt aatagtcatc ttttccctcc
     4561 ttgctaaaaa aactgcagcc tagtagctgc agcttcctac tatgcgattc caagtaatga
     4621 caataaaatt cccaagacga ccattccaaa aataattttg atgacgccga cttttttatc
     4681 tagtaatttg aaacagctca atgttaccaa taacggtaca attcctttaa aaatactatc
     4741 gaggatttct tggacattca ttagttcttc gcccttcatt gaaaagctca aaacggtatt
     4801 gaatttgacc atactgatcg tcattgcacc taccatgatc agacccaaga tactagcagc
     4861 ttttgtcaga atttcaatta acccgttcgc atacagcttt tcaatatatt tagagcctaa
     4921 tgaatagcct aaaacgccgc catagtatcg catcaagtaa ttaggtatat tgaacataac
     4981 taaaaagata attggtgcca aaatatttcc ggcattggct aagttgatcg ctagtcccgc
     5041 ggtaacgaca cgccaaactc cccagaacaa tgaatcgcca attccagcca atggtcccat
     5101 caaactcgtt ttgatcgcgt tgatactttc cacgtcaaaa ccagcttttt cagaattttc
     5161 cttctccatc gatgccgcaa tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa
     5221 atactgtgta tgacgttcca gcgccgcagc tcgatcttct tcacttttgt agaaatgatt
     5281 aatgaagggc atcatagaat aggcgaaacc accgccgccc atcgtcaccg gattcaccga
     5341 agcatacata gtcatcgagc gccaaaacat cttccgaacc aattttttct catctttaga
     5401 aattccatca gctaatttca ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt
     5461 acagcagcat cgctttgaac tgcatgaaca gatatatttt ttagttgtag atctctttgg
     5521 gctataacaa ttgcaataat cgttccaact gcggcaattg caatcaatgg taactcgaga
     5581 tacgccataa caacggcccc aaagaaaaac cacacagcaa tatttttact ccaaagcatc
     5641 cgtaacagca tcgccatccc aactgccggc agcagatttc cagcgactgt taatccgttc
     5701 atgatgacct ctggaatatt atccaagacg cttttgacaa catctgctcc aaataaaacg
     5761 gcaaagaatg gaataagagc ataaagcgca tagtggacaa accacataaa gaagtgcaga
     5821 ttgatgattc ctttttgatc accagatttt gcaaatttga aaaatttagc agccatcggt
     5881 ccacatacaa aagaaaaaat catattcatt aactgtaatc ctaatactgc tagtggaaag
     5941 acaatgggaa taatcgcatc cacgtttcct cccattttga ttgcgaacgc tgtagccagc
     6001 ccagcagcca aagaaggctc cgccgtacta gagattccga tattgacgac acccataaag
     6061 atggcttcta gtgacgcccc gacaatcaac cccgtctgca aatcccccat caacgctcct
     6121 gctaacggag caacaacaat cggacgactc agcatggaca tcccgaacat ttcatgtcca
     6181 gcaactccaa taaaaacggc caaagcgact acaaatgcat catacataat ttttctcctt
     6241 cctttttcta aaaatcactc aaaatagaga tagctgaacg tttttccatc gccggtgtag
     6301 gctgaaaaac ggtatctgga taaagttcaa ctaattcttt taagtcttga atttctgacg
     6361 tcgttaacat cacttccttt gataaggttt tcttatcttc cgatggcggt gtcattttcc
     6421 cagcattccc cacgttcatt aattcgattt ccgttgggaa agctttcgca acttttaccg
     6481 catctttgac attagggact aaaaccatca agcgcatgtc cttcgcccgc ggatctttta
     6541 acaaatgaat cgcgtcatca actgttttaa taatgacttt gacattcgat ggtgcagcca
     6601 tcttcaatgt catcttttga atatcgttgg tagccgcctc atcgcttgct actactaacc
     6661 cggttaaatt tagactcttt gtccaagtca tcgctacttg tccatgaatt aatcgttcat
     6721 caattcgcac tgctttaatc atttttcttc ctcctaaaaa aaatcttcct cttcatttac
     6781 tacatgtacc tcatcaagaa tggtcatctt taattcctgt tggcatttat ttaccagttc
     6841 ctgaatatcc tcctttgtta attccttggt tgccaatact atttcgagca gaattggcag
     6901 attaaagcca gacaaaatag ttacagcaat ctctttctcc aaaaaggttt tggtaatctt
     6961 ctgattgaca cttccgccaa ataaatcggt aaaaacaaaa atctgatcct gctcgttcac
     7021 ctggtttaca aatacattca actcctgttc aaaatcacac tcatccacat aagcattaat
     7081 caccgtaatc tcttgaacct tccccgtcag taattgaata ctgctaagaa agcctgaagc
     7141 taagtgaccg tgagtcgcta ttagaatttt cttcatatgg ttaattcctt tctttctgtt
     7201 cacttttata taagcaattc ctgtgccaac ttttaaaaac attggtttaa tcacgttaat
     7261 tcacattaaa aaaagtgtct aacctaaaaa aaacagatta gacacttttt tagacacttt
     7321 atttttgaat atacggtcct aataattcat agagcagcga aagctcataa gcattgactt
     7381 taaaattata tttcatctcc attgattgaa aaatacttct agaaacataa taaaattcat
     7441 cttcatctgg tttcaaatca taattcggtt cttcagattt ttctgtccga gccaagaata
     7501 aacgttccat catcaatgcc gtatgcatat agagatttag ttttacacgg ccatttaaag
     7561 tgaaatgata gtaattttca tatttagaaa tgactgtttc cacttcactc atcacgatcg
     7621 ttgggttcag aaagttcaaa cggtccgcaa ttccttccaa cgaaaagaat tttactagct
     7681 cctgaatcaa gcggttaaat tctgccggct tcatttcagg actcaaaata tgctccaaat
     7741 tggctgctcc atcaccgtct aagatgtcat aaacattaat attaggaatt gaaaaggaac
     7801 gtggaagatc cgaagtcgtg atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt
     7861 ggtcgattaa ttcacgtagt tctctgtagt ccttcgtgaa aacttcgatg gattcatccg
     7921 ataaatgatg acgaaaaaca tctttcaatt tgtccgaaat ccccaaaccc gacatacagg
     7981 aaatgataat gttgctactt tgagaaaagc cttcaaagta ttgaacattg attgaatact
     8041 tgtctgctgc atcctccgca atctgtttaa atggcgcatt catcgtcatc ttcaagccaa
     8101 tatcaagtgc aatggaagtc gtcaaattat tcagaagaag taactcccct tttaaatcgt
     8161 tcttgatctg tgtgtaaatt tgatttaaag agcccatatc tacgatcagc actagactat
     8221 ctgtattcac ttgccgtttc acgaaattcc ttgtcgattc gacaatctct gacacattac
     8281 tctctactgg catatccaac gcttcaaaaa cataattgcc gcataattga ttcactactg
     8341 cttgaatgct gctagccgta ctatctccgt gagcaagcaa caggcccttc agctcgatgg
     8401 attcatctac ataatcagat aaagtcacga caataagcag tcttagaaag ttctcgtcct
     8461 tttctgagag cggtggtaaa tgctccaaga actgattaca taagtaagtc gtccgaggta
     8521 gatttgcagc taatgtacga tttatcccgg ttaagtctgt cttatagctt ggggaaatga
     8581 cataggatct gctaataatt tctgcaacag gtccactcgt cgtcaatccg tacgtttttc
     8641 ctaaaacgga tacccagacc tttctaaaca tctgaatcat caattttcta ctcattgata
     8701 aaaaagaatc cttatcaatg tgatgcacca aatgctgtac ttcattcttt atcgaagaaa
     8761 aattttcatt gaaattacct tcatttatca gctgagtaat tttttccaaa atcgaatcat
     8821 aatcaaacgg tttgattcga tccgttttta tcttttgatt tttctttatc aataaactag
     8881 caatcggcaa gctttctact tcggattcca gcatatttaa ctgaatcaag atctcatttt
     8941 tcccaatcgt taatggttct tcttgtgttc gactatacac atccgcacaa ctgattttaa
     9001 tgatattctt cagcttacca atattcccac taaagggatg agataacatt aaagacaagg
     9061 cttctcttga aacaataata tctttttgta aaacatccgc ttcattttga aaaaataatt
     9121 gaatcagctc taatcgttct cgaatggggc gatcttcaaa agttggcaac ataacactaa
     9181 tttgaatacg ccgatcaaag gtgtctaata aataattttc acctttttcc gtcgtggcga
     9241 aaataaaacg aaccttagca gaatgccaat ttacattttc accgactgga cgatagtttc
     9301 cagtatcaat aaatagaaag agcttttctt gattttcttt cgataggcga tggacctcat
     9361 cgagaaaaag atagccgttg ttggccgttg ccaataaacc atcggtatcc ttttcagccc
     9421 ccgtaaaggc ccccttcttg tatccaaaca gtgttgccga aagcagttcg ggattgtccg
     9481 cataatcggc acagtttaag atagcaaatg gtgcatcttt ttcaataatt tgctcatgga
     9541 tagcgtattc aaaaattttt gtggctaaaa aactttttcc aacacccgtg gctccattta
     9601 ttaatacact gagtccgtta ggcggatatt tgacagcggc tatacatttt tgaatgacct
     9661 ctctcagact tccgtcatga ccaactacag aatgaaaggc ttcgtttatt tgctccgtat
     9721 tatctacaac cgcatattca tctaaaggct tccacaaaac aggctttccc ggtaatttct
     9781 cgaccttttt atcttccact aatctcgtca aataatggct ggcattctga cgagagatac
     9841 ctagattctt cgaaattttc tcggcagtga cagaagacat attccctgtt agtcgtatca
     9901 gctcatcaaa aactcgatct ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc
     9961 taaaagcata ataaactatc ggctgcaata tgtctattct atatttgtat tccaattttt
    10021 actttgaaac acaaatagtc gaatatttgc ctcagatatc tgacaaaata atttatctac
    10081 caatttagtt tttatctaat atgattttta tgttttttca tagctacaaa tattgaactg
    10141 gattcatata ttagttttat taatagatta cagaagggtt aagtatattt gcatagtttc
    10201 caattacttt atccttctgt aactttttca atcttagatt tactagcccg tctcgggccc
    10261 tattttacaa tggttttgtc cacttttctc tggtagaaaa acgtggtaga aaaaataaaa
    10321 taacacaaaa aaagcggcgt ttcatccgct tttggtagaa gattggaaaa ctcagataga
    10381 aaaaggacga tttttagctc tttagaacgt tgataagtcc ccattcttac tgagttttgc
    10441 cattatctcc tatacatctt gaattctaag aataggtcat tatagatgcc taggtatttc
    10501 gatcctagat ctttgtagac ttcaagatgg gagatcgtgt catcagatgg atagaattgt
    10561 tcgtccgacg aaatgtcttt tggcaataac gcttttgctt cttcatttgg tgtagaataa
    10621 ccaatatact ctgcattttg tgccgcattt tccggttcta gcataaagtt gatgaaatca
    10681 taggcacctt ctttattttt cacggttttt ggcataacga tattatcaaa ccaaagattg
    10741 gatccttctg gtgggatcac ataatgtaaa tgttcatttt cccacatcat atcggcagct
    10801 tcgccagaaa atgtcactgc cacggaggct tcttcattga tcatatacat tttgatctca
    10861 tctgccacga ttgctttgac atttggtgtt aaatgattca atttatttgc tgcttctgtc
    10921 agctgtgcca tattcttact attcaaagaa tagcctaaac tattcaaaga aagccccata
    10981 acttctcgtg caccgtcaat cagcatcaaa ctgtctttta attccggctt ccacaaatca
    11041 tcccaatgct taatttgatt tgcatcaaag aatttatcgt tatatacgat ccctaaagtt
    11101 ccccaaaaat atggaatcga atactgatta tctggatcaa aatccaagtc taaaaatcgt
    11161 ggatcaatat tatttaatcc tttgatcttc gaatgatcta atggaagtac tagtttttct
    11221 ttgatcattt tttggatcat atattcactt ggtatagcga tgtcgtagtt tgtcccacct
    11281 tgctggattt ttgtgaacat cgcttcatta gaatcaaatg tttcataatt cactttgtat
    11341 ccatattctt tttcaaattt cgtgatcaaa ctaggatcga tataatctcc ccagttatag
    11401 atggtaagta cctttgctcc actcatgcca cttgtttttt caagttgacg aacaccaagc
    11461 agcagcaccg caataatgat gagaatacct aaaaacagag attgtaattt tttcatcgca
    11521 gccgtgccac cccctcgcgt actcttctgt ttttcttcag ccgttttgga cggttttctt
    11581 tactaataaa gtagtatcca atgaccaata tcatggagaa caagaaaact aacgcactta
    11641 gcgcattgat ttctaaactg attccttgtc ttgcacgaga atagatctct actgagagcg
    11701 tcgtaaagcc atttcctgtg acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa
    11761 atgccataaa atatccagct atgattcctg gagaaagata aggcaaaatg atatttttga
    11821 ctacttggaa attattagca ccaaggtctc tagccgcatc gaccattgaa tcgttcattt
    11881 cctgcaattt tggcaagacc atcaagacga cgattggaat gctgaaagca atatgggaaa
    11941 gcaatacact ctgaaaacct aatccgaatc ctagtagtgt aaagaaaatc aagaaactag
    12001 caccgataat aacatcagga gagactaata agatgttatt catgctcaac aacgctgtcc
    12061 gtgcacgtcg ttttttcgtg taatagattc ccatagcccc aaatgtcccg atgatcgtgg
    12121 cgatcaatgc agataagaaa gccagcatga acgtattcaa tacgataatg atcaaacgtg
    12181 tatcttcaaa aacagcttca taattttccc aagtaaatcc tgtaaagctg ttcatcgttc
    12241 ctccttcatt gaaggaataa aaaatcagat agaagatcgg tgcatataag aataaaaaga
    12301 cgatccctaa ataaagatac gaccatttaa attttttcat ttatgagcga cctccttttt
    12361 tcttctcgcc tgtcaggagc atcacgatga acatagcaat gattaaaatc acaccgattg
    12421 tagaacccat cccccagttt tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag
    12481 tgatcacccg atttccgcca atcagacgag tcagcatgaa taaagaaaga gaaggaataa
    12541 atactgcttg gacaccactt ttcacaccat ttaatgataa cggaaaaatc actcgtcgga
    12601 aggtctcaaa gctattcgcg cctaaatctc tactagcact gatcaaagaa ggattcatct
    12661 cttccaatgc attgaaaatt ggcataatca taaatggaat ctcgatatac gttgccacga
    12721 acagaaaact gaagtctgtg aataatatct gtttcggccc gatcccgata aaatcaaaga
    12781 aatgattcac gctgccgtgc acactgaaaa tcccaataaa tgcgtaagct ttaagcagca
    12841 gattcaccca tgtaggcaaa ataatcagca tcagccataa ttgcttgtgc ttcagttttg
    12901 ttaaaaaata agcagtcggg taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat
    12961 accatacaga attgatcgtc atagaaagcg atgtccccga cgtaaaataa gatagataat
    13021 tattcagcgt gaattgccca ttcatatcaa aaaaagattg ataaatgatc attaatacag
    13081 gcgcgatcac gaataaaccg agccacaaaa tataagggac ggaatagatc ctgcgcattg
    13141 ttttcattct tgtcccccct attcttcgta gctgtcaaga cgtgcatcga attcttcttc
    13201 agactcgttg aaacgcatga catggatatc ttctggctca aaggccaatc ctactttgct
    13261 tccttcttta gcttttttcg ttgaatggac catccattca ttttgctgtt catcataaca
    13321 gatgatttca taatggacac cacgaaacag ctgagtatcg accgttacga ctagttttcc
    13381 tttatctggt gtcgtgatcg tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt
    13441 tagacgcatc ccgccatcga cacattcaaa ttgcttacca acaaactcaa ctagattatc
    13501 ttcgatcatt acaccgtcca cgatattgct ctccccaaca aaatcagcaa cgaaatgatt
    13561 gattggctca tcataaatat ctacgggtgt accactttgg acgattttcc ctttattcat
    13621 gacaaaaatc tcatcactca ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa
    13681 cgtgattcct aatcgttgtt gcagatcccg cagttcatac tgcatatctg tacgtagttt
    13741 caaatcaagc gccgataacg gttcatctaa tagcaatact ttcggttcat ttacaattgc
    13801 tcgtgcaatc gctacacgct gtctttgacc accagacatt tcactaatct cccgtttttc
    13861 gtatccagga agctggacca tacgcaaagc atcttttacc ttcttttcga tatctgattt
    13921 ggacattttc ttgatcttca atccaaaagc aacgttatca aaaacattca tatgaggaaa
    13981 aagtgcataa tcttggaaga ctgtatttac ttgtcgctta ttagctggca aatcattcaa
    14041 ccgtttccct tcaaaataga tgtccccttc tgtagcttcg gtgaatccag ctataatacg
    14101 taggatcgtt gttttaccac agccggaagg accaagcagt gtataaaatt ttccttgttc
    14161 gatctcaaaa cttacttttt ttaatactgt ttcatcatca tagcgtttca ctacttgatc
    14221 aaatgaaatg attggtttcc ccacgttttt accccctata ttacaaatat gattgcgttg
    14281 caacaatcaa tacttttgtt cgttttttga gatgattcct taattgatgt tgttttgtcg
    14341 cttgataata gatcgactgt cctgcttttg ccacatagac cgtctctcct aagcttaact
    14401 ctaattcccc ttctaataca aaaataaacg tttcagataa tgaaggttca aacgtcttat
    14461 attctcctgc accttcaaaa gtcagcaaga caggctccat ttctttttca ttcgaatcag
    14521 ccacgagcca ttccaattca tacccgtttt cttcatcgaa ataaaccgta tgatcttctt
    14581 ttgaataaac cacttgcaga ttcgctgctt cttgatgaaa aaattcttct ggagtcacac
    14641 ctaagacttc aagaatagaa aagaatgttt ccattgatgg tgaacttaaa tctcgttcca
    14701 actgggaaat ataccctttt gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat
    14761 tcttttggat gcgcaaattt cttaacttct caccaatttc cataatttcc tccgaatcga
    14821 agttttctaa tagtaaactt cctgtttact accactcgta ttagtatacg aaatttattg
    14881 aaaaaggcaa ccctttgtaa aatttttttt gctgaatatt tctttatctt ttggaataaa
    14941 ttctagttgt ttgattcttt atttttcacc taatttttgt ctaaatttac ggaagaacaa
    15001 gaataaaaga gtaggcttta tggtatgata aaagaaaatt ttaaaaggag tgtctcacga
    15061 atgaacgctt ttcgatcttt tcaggtaata aaggaacctg ttttccatac agcaatcagt
    15121 gatctgccat ttactgagtt ggctgaaaat gaagtcttgg taaaaattgc ctattctgat
    15181 gtcaattata aagatgcatt ggcaatgagc gagtctggtc aggtgatacg aacatatccg
    15241 atgactccag ggattgattt atccggaact gtcgtccaaa gtaacaatcc acgtttttca
    15301 aaagaagatc ttgtacttgc tactggtttt gggcttggcg tcacccatcc aggcggttat
    15361 agccaatatc aaaaagtacc aggagactgg ttagttcctc taccaaaaaa tatgacgctg
    15421 cgtcaagcga tgatccttgg tacagcaggg tttactgcta tgttgtgtgt gaacgcttta
    15481 atccgtcaag gtatgacctc cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta
    15541 ggcagtacag cgatagccct gttacataaa ctaggattcc agtcaatcat taccttttcc
    15601 agaaaagaag agtctgtcac atggctaaaa agtctaggtg ctttgcaagt cgtacgacct
    15661 gatgaatttc tgccagagac aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc
    15721 gacacagtag gtggcgagca gctaagcagt cttcttccat tgatctctta taacggtgca
    15781 gtagctttat gtggcaatgc tgggggaatc aaactaaacg cgactgtctt gccttttatt
    15841 ctgcgaaaca tccagttgat tggaattgat tctgtcaatg taccaataga tcaacgtctt
    15901 tctttatggc aacagatggc ggatttgcag attgcagatg aactggtcgt ccaagagatt
    15961 actctggatc aattgcctga aacagcatct aaactacttg ctggaacaca ccaaggaaga
    16021 acactagtaa atgtaggtga ccataaatga agattctaat cacagctgga ggaacgactg
    16081 aaaaaatcga tcaagtacgt gctattacaa atcattcgac aggacgatta gggcaagcat
    16141 tagctgatta tttagctgcc aatccggata ccacaatcga ttacgtgact acgaaacaag
    16201 cactgaaacc gaaaagacac tccaatataa cgatttatac gatcgaatct gcactggact
    16261 tatttttaca attagaagaa ctaacgaaaa aagaacacta cgatgcgatt atccacagta
    16321 tggcagtcag tgattttact cctgctttta gtttttcaga agaacagctg gcaaaaaacc
    16381 tacccacttc ttcaacgcaa gaagaactgg acaattggtt tgcggaaaat gaacagacag
    16441 ataccactgt ttcaaagatc tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa
    16501 caccaaaaat catttcttat ctacgtgaat ggcagccaaa agcaaaaatc attggtttca
    16561 aactattagt agatgtacca aaggaatctc ttctggcagt cgcaaaaaat agtctgataa
    16621 ataacaaaac tgattttatt ttcgcaaatg atttgacgga aatccatggc gagacacacc
    16681 acggctatct cttatctaaa gatggaacag tggaagaagc tcaaagtaaa tctgagattg
    16741 ctgcattgat tactgagaaa attagactgg aggaatcaaa atgaaaaaga tcttactagg
    16801 cgttactgga agtatttctg cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt
    16861 aggtgcccag gtagatgtga ttatgtctaa aagcagcaca cattttatca cgccattaac
    16921 cttgcaatct ctgtcgaagc gcgcagtcca tacagacgtc atgcaagaat caaatccgca
    16981 ggtaatcaat catatcgaac tagcaaaaca agctgacttg tttctcatcg cgcctgctac
    17041 agcaaacatt atcggcaagc tagcaaacgg cattgcagac gatcttcttt caacagtcgc
    17101 aatggcgctt ccagtgggaa caccaaaatt gattgcccct gccatgaaca cgaatatgta
    17161 tcaaaacccg atcactcagc gtaatctgga aacattgaga tctgtcggct atcaggaaat
    17221 tgaaccaaga gaatctcttt tagcttgtgg agattttgga aaaggtgcct tagctgatat
    17281 tcagacgatc atcgaacagg tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg
    17341 agtacttatg aaaaatacaa aaaactttac attaactgcg atgtttttag ctattttgat
    17401 tttattagct gttacgcctt taggttttat ccctatcggt ccaatcaatg caacaaccat
    17461 gcatattcct gtaatcattg cttcgatcgt tttaggacca agactaggtg cttttcttgg
    17521 cggtacattt ggactgatca gcatgatccg tagcacagtt atccaaacac ctttgtcttt
    17581 tgtcttttct ccgtttatcc cagtcatcgg aacagaacat ggaagctgga aagccttgct
    17641 gatcgcgttt attcctcgca tcttgattgg ggtggttcct tatttcgttt ataaaggtct
    17701 gcgcaaacta atgaaacaaa aagcagattc cgtttcttta ttcttagctg gactttccgg
    17761 ttcattgacc aatacgattt tagtgatgaa tatgatctac tttttattcc agcaagatta
    17821 tgccaaagtc atcggaacaa atttaaatgc tgtttattca gctattctag cggttatttt
    17881 tacaagtggt atacctgaag gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt
    17941 cttgttaaga tacttcaagc acccttctct tactaactga tagtcatcgt agccgatctc
    18001 tgattttttt ctcttttggt gttactattg aatggaaaga tttgacgtcg aaaggatgat
    18061 ccaaatgaaa atttttgttg taggtgcaaa cggacaaatt ggccgacacc tgataaaaga
    18121 tttagctcca agttcccatg aaatttttgc aggggtgaga gatgttgctt cccaaacatt
    18181 agtaaaaaaa gagaatgttt cgtatgtctc ttttgattta acttggtccg tggaaaaaat
    18241 gaccgaagca ttcaaaggca tcgatgtatt gatttttgct gccggctctc aaggaaagaa
    18301 tcttctgcaa gtagacttgg atggcgcaat caaaacagtg atcgcagctg aaaatgcgca
    18361 tgtctctcgc tatctgatgg tcagtgctgt ttatgcagat gaaccagcaa aatggcctga
    18421 gtccatgaca gattattata ttactaaaca ttatgcagat gaatggctga aacgaactaa
    18481 cttagatttt gtcattcttc aaccagtcac attgactaac gatgaagaag ttacttccat
    18541 tcaattaact aagcctaatg agaaggcatc aaaaacgatc actcgctcta cagttgctgc
    18601 tgtacttgcc gctctagtgg aagaaacgga tatttctcgt acgactctag tactttctga
    18661 aggtagcaaa gagttgaata cagcattcca agaatgggca aaggaggaat agatccatgc
    18721 gtgccatttc aatcaagcat cctggttctg tagatgtatt agaacaaatt gaagtgcctg
    18781 ttcctaaacc aaaacaaggc gaattactga tcaaagtcca tacagcagct gtcaatcgaa
    18841 cagatattat gaaaagagaa tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg
    18901 aagtagctgg agaggtagtt gagaataaaa gtgatatctc cacttttact cctgggacaa
    18961 aagtatacgg attagtcaat ctaggtggtt atgccgaata tgctgtgatg cctgccgatc
    19021 gtgcaatcct tctccctgat acgctagact atgtttctgc tgctggaatc tcagaagttt
    19081 ttttgactgc ttatcaaaca ctttactggc ttggaaaatt acaagaaggc gaaactgtct
    19141 tgatccatgc aggagcaagt ggtgtgggaa cagcagcgat ccaattagct agacagcttt
    19201 ctcacgcgaa aatcatcgtt actgcaggat cagaagaaaa actcgccttt tgtaaagaac
    19261 ttggtgcaga tgaattgatc aactataaaa aacaagactt tcaagaagag gtacgaaaaa
    19321 tcactgaagg tcggggagtc gatgttattt tggatttcat cggcgcttct tactgggaaa
    19381 agaatttagc ttctatagcc gttgatggac gttgggtctt gattggtatg ctaggtggga
    19441 ccatcgtgcc tgagatagac cttggtgtat tgattgggaa acgtgtccaa ctgcttggta
    19501 cattattgac acctagaagt gatcagtata aagcagagct cgcacaaact tttatgcaaa
    19561 aagctgggcc ttttctagaa aatggcaaga ttcggccaat cattgaccgt acattccaat
    19621 tcagcgcagt caaacaagcg catgaatata tggaagcaaa caaaaacatc ggaaaaatca
    19681 ttttgaagat aactgaataa aatcatgctg atcaaaaaag aggctgggac agaagcgttt
    19741 aactccaaga aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttcag
    19801 cttatttccg aaggaatttc ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca
    19861 aaagtgttct ttacttttgt cccacgctct ttttccgaat aaacggtgtt caaaagcttg
    19921 catctgcggt aataagtcca accaagttaa aaacataaga agctgttttt acttggctgt
    19981 tcttattact tgatgcaggg cagctttttc acaacctctt cactaaacag tgttcaatca
    20041 gctgaccctc ggtattagct caaaacgctg atttcacaac ctctttttat ttacgttcta
    20101 tttcattcat tttccgaagc aaatcgagct gcttgtatgc ccgcttgtcg cccaaaaata
    20161 atgatctccg caactgaatt tccaccgatc cggttgtttc catgtaagcc acctgtgact
    20221 tcaccagcag catataaccc cctaattggc tggttttctt tatctaatac ttcagtgttc
    20281 gtattgattt tcacaccgcc catcgtgtaa tggattcctg gtgcgatttt gatcgcataa
    20341 taattcggag acgataagtc atgttccatg gctgtggcac ggttgaattg agcatctttt
    20401 ttctccgcaa catccgtatt ccatccatca actgtttttg ctagtgtttc ttttggtaaa
    20461 tcgatttttt ctgctaattc ttccattgtc tttccagttg tgacatatcc tttttggtca
    20521 taaaattcaa tagctgtggc acgatcacgc acgccttggt caaaaatcaa ataagctgat
    20581 ttttcaggca atgctgtaat tgctgcagat actttatccc ttgtatccat ttcattgaca
    20641 aatcttttcc cctcttgatc gaccaagatt gctccttctc cgcgaacaac ctcaccaatc
    20701 aagaccccct catcctgctg cacagttgga tgaatttgta ttttatccat atcaacggtt
    20761 tgtccaccta atttttcaat catggtgata ccatctccag ttgatccttc ttgatttgtc
    20821 gtaatataat cagctaaatc gggacgatat ttttcaataa attctttgct ggcaccaaaa
    20881 ccgcctgtgg taactacgat tgcttttccg gtgacctctt ttggttcttc tccttgaaca
    20941 tgtacagtca cttcattgac cgtttcccca tttttcttaa tgtctgtcac atctgcgttg
    21001 acaaaaatag ggatttcccg ctcatggacg ttattcaata acccctccac taagtatcca
    21061 ccaatagccg agccatcaga cggacgatgt gtcctctttt cactcatccc tccagtgatc
    21121 gttaagttgt ccagtttgat accgttttga tctagccaat cgaccgcatc agcagagtga
    21181 tcgacaaagt atcgaagcat ttctttatca ttcgttcctc caccaccttt tagcgtttct
    21241 tcatagaagg catcattcga gtctttgatt ccttcttctt tttgaaattt tgtttctgaa
    21301 gcattcatac cgcttgatga tttagatgtg tttcctccgg caacaggcat cttttccaaa
    21361 attactggat tcaacccagc atcttttgct tcgatagctg ctgccatccc tgcaccacca
    21421 gctccaataa tcacgacatc ataagattct ttcatttctt ttggatctgt atatacttgt
    21481 tcagaagcgc ctgatgatac ttcggcagat tccttcgtac tagaacttgt ctgcgctgta
    21541 tttttctgat ctgcgccgca tgcgctcata gcaagaagtg caacggctgt taataaacca
    21601 gcaactaatt ttttcatttg tatccccctt ttctccttat actatattaa gtgatttttt
    21661 tcacaatatc aactctgttt ttctttaagt catatagctt tggtaaacac acgcttctta
    21721 gtataattaa ggatatacat gaaggagtta ttttatgaat catttgaaaa ccttttttac
    21781 gaacagtaca aaaactgcag gtagaatcac aaacggaatc tatattttat tacgtatcat
    21841 cgtcgttatt atgctagtgg atcaggtctg gaaccaacac tggggaaata caatgctttt
    21901 attactgaca ttaattttat ttgaaatccc ttattttctg gaaaagatat taaagataca
    21961 gattccaaat atgctagaaa tcattttgtt atcttttatt tttagttcaa cagttttagg
    22021 agagttaagt gatttttatg gctatttttc aatgtgggat acttttctac atgcattcag
    22081 cggattttta gctgggggtg tcggcttttc ccttgtttat ttgctcaata aaaatacaaa
    22141 aaccatgaac ttgactcctt tattactagc agttgtttcc ttctgttttt cgatgacagt
    22201 tggtgttatg tgggagtttg tggaattttc agcagataaa ttactgcaat tcgatatgca
    22261 aaaagatacg atcatccagc aaatcaatag tgtggagtta agtaaagacg gaaacactgt
    22321 ctatcatgtt ccagatatca ctcggacaat cattgaaagt caaaatgcag atggtaaaac
    22381 agataaaaca atcatccaaa atggttactt agacgtggga atcatggata ctatgaagga
    22441 tttatttgtt aattttattg gagcagttgt tttcagtgta tttggttacc tctatagtcg
    22501 aaacgattac caaaaattta cgttcatcaa gaattttatt cctaaaaaaa tcaaacgata
    22561 aaacatgcta aaaaacaaga gaaggggcag tgacaatact tttgtcactg ccccttcttt
    22621 tttgaatgta tggcatgcca ttagttatcc gatagtatta actcaaaaaa gtaagatatg
    22681 aggaatgact atttcgtttt ttatcccaat actgaggact tatttcacca tttcgttatt
    22741 tatctaattt acttaaaacc tgcataactt tttcataatc tggattaggt gtgccgtcta
    22801 gattttcagc aataaaagta aaacccattt ttttataaag agcaatcgcc tgatggctcc
    22861 atgtctgtgt gtgaagatag attggtccgt ccgtatggag ggtaggaaat tcttgcaaga
    22921 ctctggcagt caatgctttt gcgagccctt ttccttggta atctggtttc actgctaacc
    22981 aatgaaaaac cggatacact tgctctcttg atttttttcg ccaagcggta caggttgcta
    23041 ctttttctcc tttttttgtc tctacaaaaa acatccttct ttcaagttct tcaggataag
    23101 gagcaaagtt ttgttgaaaa tactcaagtg cttcatctgt cgtttgaaat tccccaacgg
    23161 aagtctcaat ctccgcccaa gcagattcat cttctttttg ataaaatcga aattcaaaac
    23221 cttccgctaa aggcgtgatt ggaacatttt gttgaagtgg tcttgtcatc cagatttctg
    23281 cgtaaggtaa cgttttatct aacatttttc tatccctcct cgtctccatt atgccaaata
    23341 acatatgact cagctactta tttgactata acagatagag atataaatca gaaccagtca
    23401 tctgcgtcaa attcatttcg attagcgtat acccatgttc ctgccatacc tcgcatgaaa
    23461 tctcctaaag cagaagaagg aatacctcca ccaggtgcgg gtgttcccgg actaggaagt
    23521 ggtacttgcg gctgcagatg ctcatcggtg gaatcaatag ttgacaagcg ttttttcttc
    23581 gtttccttct ggtcatctga ttccttctga tcttccgact gtttagcaac aaattctttt
    23641 tctgattgtt gttttatttt aggctctaaa atttcatttt ccaatccaga aacttgtggg
    23701 gccaaatgag cttcttgcct tatcgtttta ttagattctg agatttttct agatattctt
    23761 gctaactcta ggcttcttgt ttgttttact ttccctgttg gagaaactgc atgagccatt
    23821 tgttgttttt ttctgacaag ctttcttctt gtcttttttg ttctttttga tctatctgtt
    23881 tgtttaatgg taaaacatgt aatagtgact gagcatggag ttcttggagt gaatacgggt
    23941 catgataact atacataatc gtaaaatcac ctgtttgttt ggtcaaataa ccatcctcca
    24001 atggtacttc attgattgta accgatacat cctttaacgg tacgggttta cctaaggaat
    24061 ttgttgctga ttggatcaac tcttctttgt ttattttttc gtttggatga acaataagat
    24121 catgaccttc taatgaacga ttgtctggta tataaacata agtaaaggta atttcccctc
    24181 ctgattgctt cctattattc atcagttctg agtgaagtac ttcaaggatc tccgaccagt
    24241 gactttttct gtctaatccg tttgcttgca tccaatcaga aatagaagtg actttcccat
    24301 tttgttcata agtcgataaa tctatatttg gcactcgatc agaaaattca gcaggtaggc
    24361 gcaaatcagg aatacgattt acactactca tatgtgtttc tgcttgaagt tctttatttg
    24421 tttgactatc tataaaatga gtgatgacag tcgatgaagc agcagatgct tctacataaa
    24481 ctacagctgt tgtttctgcc acatttgatg ttttcgagtc tatataagtt aatacaacgg
    24541 gatattctcc cggagttgta gtatctactg tcccgttcat tttgatttct tctttatttc
    24601 ctgaagttcc atcgcttttt gttacatatt caacaagatc tttaggggcg taactctctc
    24661 ctgttcttaa gtgaagatct tttcctttga tagctgtttg atcagcacga acatggatca
    24721 cagcagatgc agtcattgtt tttaaagcaa ttggatcaaa ataggacaag gaaatagtag
    24781 atgttcctgt attagtcgta tccacgatat tcccattgtt tgaaattact ttggacatat
    24841 ctccttgtgt tccatctgcg ttcttcagcc tttctaccag atcagatatc gaaacttttg
    24901 tccctgccag aacatcgatt ttctttcttt caaactgtga ataatctttt tcatatacat
    24961 aagaaacttg tgcgcttcca ccgtccttta tatctgtcaa agcatgattc aaaatatcag
    25021 tgatctgttt taactgatca ccttttgtag gatcagtcat atcggaaaag atagaaagcg
    25081 gcacactgct tccggtttcc gatgtccata atgttttgtt aggaattaac cagtagtggt
    25141 ttatcgttgg aggataactg actttattat aggaagtcaa tgcaggaagt agctgttttc
    25201 catttgcatc agtgaaagta tgagtaatag tgatagggga atcagtggtc tcagaaacag
    25261 aggtacgtac gaccttgatg gtttgtgtcg ttgcctgaac gggtacatgc atatagacac
    25321 ctacagataa actgatcagc atcgtgcgca tcataaactt aacgtagtgt ttttcagaac
    25381 acgaccgttc cttatctaaa cgacaagcgt gtttatttag ttgttttgaa cgtctttttg
    25441 aacacattta ttcttagtcc tccctactct tgtctaaaca tgaagtagta attttcataa
    25501 gatgatttca ttatataaat aaaatcaaaa gaagatggac tattaggaga gtattttttt
    25561 tgatttttta ttgtttcaag aaaatcggaa taataactta tgtacttttt aattttgtta
    25621 agtaatatat ctagttatat ctagttatat ctagttacct tttctttgaa aaaaacataa
    25681 at
//
LOCUS       DAZUJO010000033        24972 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000033 DAZUJO010000000
VERSION     DAZUJO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.033"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..794)
                     /locus_tag="ACG8OL_001475"
     CDS             complement(183..794)
                     /locus_tag="ACG8OL_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS4817469.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(917..1042)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1124..1840)
                     /locus_tag="ACG8OL_001476"
     CDS             complement(1124..1840)
                     /locus_tag="ACG8OL_001476"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4817470.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1846..2415)
                     /gene="scpB"
                     /locus_tag="ACG8OL_001477"
     CDS             complement(1846..2415)
                     /gene="scpB"
                     /locus_tag="ACG8OL_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS4817471.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2399..3169)
                     /locus_tag="ACG8OL_001478"
     CDS             complement(2399..3169)
                     /locus_tag="ACG8OL_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS4817472.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3166..3537)
                     /locus_tag="ACG8OL_001479"
     CDS             complement(3166..3537)
                     /locus_tag="ACG8OL_001479"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4817473.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3556..4443)
                     /gene="xerD"
                     /locus_tag="ACG8OL_001480"
     CDS             complement(3556..4443)
                     /gene="xerD"
                     /locus_tag="ACG8OL_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS4817474.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4539..5012)
                     /locus_tag="ACG8OL_001481"
     CDS             complement(4539..5012)
                     /locus_tag="ACG8OL_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4817475.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5117..5980)
                     /locus_tag="ACG8OL_001482"
     CDS             complement(5117..5980)
                     /locus_tag="ACG8OL_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS4817476.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6176..8275)
                     /locus_tag="ACG8OL_001483"
     CDS             complement(6176..8275)
                     /locus_tag="ACG8OL_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS4817477.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8495..9604)
                     /gene="rpoD"
                     /locus_tag="ACG8OL_001484"
     CDS             complement(8495..9604)
                     /gene="rpoD"
                     /locus_tag="ACG8OL_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS4817478.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9628..11469)
                     /gene="dnaG"
                     /locus_tag="ACG8OL_001485"
     CDS             complement(9628..11469)
                     /gene="dnaG"
                     /locus_tag="ACG8OL_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS4817479.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11651..13966)
                     /locus_tag="ACG8OL_001486"
     CDS             complement(11651..13966)
                     /locus_tag="ACG8OL_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4817480.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14151..15011)
                     /locus_tag="ACG8OL_001487"
     CDS             complement(14151..15011)
                     /locus_tag="ACG8OL_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS4817481.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(15070..15702)
                     /locus_tag="ACG8OL_001488"
                     /pseudo
     CDS             complement(15070..15702)
                     /locus_tag="ACG8OL_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15869..16630
                     /locus_tag="ACG8OL_001489"
     CDS             15869..16630
                     /locus_tag="ACG8OL_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4817482.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16656..19247
                     /gene="mprF"
                     /locus_tag="ACG8OL_001490"
     CDS             16656..19247
                     /gene="mprF"
                     /locus_tag="ACG8OL_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4817483.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19435..20622)
                     /gene="tuf"
                     /locus_tag="ACG8OL_001491"
     CDS             complement(19435..20622)
                     /gene="tuf"
                     /locus_tag="ACG8OL_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS4817484.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20990..21346
                     /locus_tag="ACG8OL_001492"
     CDS             20990..21346
                     /locus_tag="ACG8OL_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4817485.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21587..22591)
                     /locus_tag="ACG8OL_001493"
     CDS             complement(21587..22591)
                     /locus_tag="ACG8OL_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS4817486.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22757..23608
                     /locus_tag="ACG8OL_001494"
     CDS             22757..23608
                     /locus_tag="ACG8OL_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4817487.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23625..23870
                     /locus_tag="ACG8OL_001495"
     CDS             23625..23870
                     /locus_tag="ACG8OL_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817488.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            24057..24761
                     /locus_tag="ACG8OL_001496"
     CDS             24057..24761
                     /locus_tag="ACG8OL_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817489.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7027 a         5175 c         4362 g         8408 t
ORIGIN      
        1 ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc aaagaattga
       61 ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac gtttacatta
      121 ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga tctttttgat
      181 atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact taaccatgga
      241 agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt gatcaagttg
      301 aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat ctgtgttgcg
      361 ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc gatactcata
      421 aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct tcctttatgc
      481 tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt tgataagaaa
      541 ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag atgtaatact
      601 gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat gctgatcaat
      661 accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat cggaaaagca
      721 aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat tttctgtaca
      781 cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac tttttgacgt
      841 ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt tatttgatca
      901 agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta tcggaatttc
      961 accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg tcgggaattt
     1021 caccctgccc ctgaagacga accttatata atttttgttt acatattcat tatacatatc
     1081 tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt gagtggttca
     1141 gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt aggccttgta
     1201 aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca actgcttgaa
     1261 gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg atactgtggt
     1321 ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt cgtccttcga
     1381 tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct tttacctttg
     1441 caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca aaatctccat
     1501 catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca ggatagatac
     1561 gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca tctgcaacag
     1621 cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct tgataaattg
     1681 ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat tcccttacaa
     1741 cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt cgacgagaag
     1801 tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt ctcctttatt
     1861 cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt ccatttcttg
     1921 tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt agccagttgt
     1981 tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa tcagtttatg
     2041 ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct cgatttcagc
     2101 tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg cttgagataa
     2161 tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt ttttagtcgt
     2221 taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt tttgagtgta
     2281 agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat ttaacagtcc
     2341 gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg cttcaagttc
     2401 acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt gattcatagt
     2461 tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa gccataaaag
     2521 tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta cagccatgcg
     2581 catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt gtgtcatcgg
     2641 gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt ttctgcatgg
     2701 cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca gcatccgcta
     2761 acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct gctttttctg
     2821 tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg accaattctt
     2881 ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg acaggaagca
     2941 atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct cctgcaacag
     3001 ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg atcgtagcga
     3061 tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc agatcgagag
     3121 gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact ctttcttctt
     3181 tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac ggatggagac
     3241 atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct caccgcgata
     3301 agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc agtaaatacc
     3361 aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt cataatcgtc
     3421 ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca gtttcttatg
     3481 gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg ttaacatctt
     3541 ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg tcattctttt
     3601 cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta acaattcctg
     3661 tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat gccgcagcgt
     3721 atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca gatttttcca
     3781 gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca tttcagatgc
     3841 gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt ggatcgcata
     3901 atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta ccagtccaag
     3961 agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca tcccagtagc
     4021 atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg tatcaggtgc
     4081 ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct gtgctttctt
     4141 cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc gtaaaaactg
     4201 atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt ttttttcttc
     4261 atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc atgaagcaat
     4321 tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc tttgtcgtgt
     4381 attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga tttgctcttt
     4441 cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc tgatagtctt
     4501 tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg gtttcttttt
     4561 tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc ttttactaaa
     4621 aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc ttctacttcc
     4681 tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc gccttctttt
     4741 ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat tttcaattct
     4801 gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact cttttttttc
     4861 actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa aagcacaagc
     4921 agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg ctgttttgtt
     4981 tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat ttataataat
     5041 tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct agttaagaaa
     5101 aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg ttttcttgtg
     5161 taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct ttgctgatac
     5221 caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt tgctgatctg
     5281 cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct tcatatgctc
     5341 ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact cgacctttta
     5401 tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac ccaagataga
     5461 aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt cctatcacat
     5521 ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt ttttcatctg
     5581 ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc aagcgatccc
     5641 cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg acaaagtctt
     5701 tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca gtaacgattc
     5761 caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga ttttcatgtt
     5821 tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca ccttctgatt
     5881 tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca ttttcatcaa
     5941 taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc ctctatagtg
     6001 aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac ttgaatagct
     6061 gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca tactcccctt
     6121 tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc taaaattaat
     6181 tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg taaaatggca
     6241 aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca tatagattat
     6301 aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct tcaacgatat
     6361 atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc cacgtccatg
     6421 ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc tgattcgctg
     6481 agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt gcgaccgcag
     6541 cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct tgttcttcag
     6601 ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc tctttatcag
     6661 tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga tctgtatttt
     6721 tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct gaatccacta
     6781 cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat tcagcatatt
     6841 gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg ttatccgttt
     6901 cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga tgatcatcga
     6961 cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc ttagattgga
     7021 tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga ctggataaat
     7081 cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat tgatggtgat
     7141 catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc tgtctttttg
     7201 cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc cagcttcctc
     7261 caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat tttttctttt
     7321 gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt tcagtatctg
     7381 gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc tcttcttttt
     7441 ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca gcatcggatt
     7501 cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt atttcttttt
     7561 tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca gtaaatactt
     7621 cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct ttacctgtgg
     7681 cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct actttgtttt
     7741 cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct tcttttgtat
     7801 cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca ttatcgacga
     7861 tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct tcttgattct
     7921 ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt ttctctatat
     7981 tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt tcgaataatt
     8041 ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga tcgtttaatt
     8101 ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca tctgtatttg
     8161 tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga ccgcagtctg
     8221 gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt ttcatcactt
     8281 agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt ctatacgtca
     8341 cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc ttcctataga
     8401 aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa accaaacagt
     8461 tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct tttaattgtt
     8521 ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga cgaatacgtt
     8581 cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg ccatcatcta
     8641 aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct agtacatctt
     8701 ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt gcttcctggt
     8761 cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt tcaagggaaa
     8821 caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga tccatttccg
     8881 caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga cgttggatac
     8941 gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta cgtgcttggt
     9001 cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt gaaaacttaa
     9061 atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt ccttcttgga
     9121 tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcgatactt actactaaac
     9181 gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg attttcaatg
     9241 ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct ttcagataca
     9301 tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct gtttttgctt
     9361 gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca ttttcatcca
     9421 ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca tctgcattca
     9481 aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt cgtttgggtt
     9541 tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct gttccatttg
     9601 ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt tcgttaaatt
     9661 gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc tggcttcttg
     9721 ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga tcacatctag
     9781 caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt cgatttccac
     9841 caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa ggttgaattc
     9901 ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg catcatgaat
     9961 aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa atgcacgata
    10021 taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc gtgcagtttc
    10081 ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt gctgatttgt
    10141 ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa actctgttga
    10201 cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa gttcaactag
    10261 taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc cttgttttaa
    10321 ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt cttggaaaga
    10381 tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa cgactacgac
    10441 agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg ttgcttcaaa
    10501 acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt ccatttgttg
    10561 gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga taccagcctg
    10621 ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt ttttccggat
    10681 atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca gttctgtttc
    10741 tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg caagccagcg
    10801 tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca ctctttgata
    10861 aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc gttctaacaa
    10921 ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct cattaggtgc
    10981 aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt gcaaatattc
    11041 taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt ccgctgcttt
    11101 ttcatgagca gcaatcagtt tccccgtagc agattcagga ttttggacag ctatagtacg
    11161 ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta ccactgcttc
    11221 tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc ctttaccaca
    11281 accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg tacgttcttc
    11341 atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga catattgtcc
    11401 aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt gaggaatccg
    11461 ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca gaagatattt
    11521 caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt aaaggacaca
    11581 attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca catttctatc
    11641 tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca gccttccagt
    11701 acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct acgctctctc
    11761 ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca tatatcccta
    11821 cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa gcttttacaa
    11881 ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt tcagcatttc
    11941 cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact acaagcaagg
    12001 catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt tttcttttat
    12061 cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca atcagcgtga
    12121 tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc gataaaataa
    12181 atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg ttgaccactc
    12241 ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac aataccagat
    12301 tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa tctgcttctc
    12361 ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg ccgctttctt
    12421 tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa accgtatagc
    12481 gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg acatacgcat
    12541 cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga ttgtctccag
    12601 aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct tgagcgtttt
    12661 ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct ggtttttctt
    12721 cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca ttcgcttggg
    12781 ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc gctcctacgt
    12841 acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa gaaaatgcga
    12901 ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata gcccgcatag
    12961 tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg atggctttct
    13021 cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca gtaatggtcc
    13081 ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa tacatgtctt
    13141 gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca atacttatta
    13201 gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca ataacagaaa
    13261 ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc cccaaaggaa
    13321 taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt tcagaacgaa
    13381 tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct gcacctactc
    13441 ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc aattcagctc
    13501 cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt gcttctgccc
    13561 gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt tttaccaaat
    13621 ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg atcactagta
    13681 cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt atttcttgat
    13741 aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg gaatacgcac
    13801 ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac gtcaatatat
    13861 tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca ttttgctggc
    13921 cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc tttttctcca
    13981 ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc aagattaaaa
    14041 acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa aaaagatgcc
    14101 aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct ttacttatat
    14161 tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa aagaacagga
    14221 caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat cataatcgtt
    14281 gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt tgagaaaatg
    14341 taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc gatcggcaca
    14401 atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa tcccatatac
    14461 accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat caatccgccg
    14521 gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg agcgccgtat
    14581 aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc agaagtatct
    14641 cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat acatccgaca
    14701 aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa aaaagaaagt
    14761 agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac attttctttg
    14821 aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc aaagacagct
    14881 gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa cattcccttc
    14941 acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc cattttttgc
    15001 cttttttcca ctatttgcgt actccttctt tcatttaatt ttattcatga ttgaacattt
    15061 cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat tcattttgac
    15121 aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat tgtaaagcaa
    15181 ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa gtgggctcag
    15241 cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat gattttttac
    15301 ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata aatcgcatcc
    15361 ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat caatactgtc
    15421 agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct gcatgagatc
    15481 tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct cgtagttttc
    15541 catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca ctcgttttac
    15601 cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca gaatgatcat
    15661 gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac ctagttattt
    15721 tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc cttgatttct
    15781 gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac gaaaatgatc
    15841 gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac tttacacagt
    15901 aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga gcgaaattta
    15961 ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg acggcagcgg
    16021 ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt atctagtaga
    16081 tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc agttaatggc
    16141 cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt ttcttggatt
    16201 tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa aagttcatcg
    16261 attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg ccagagtcat
    16321 cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa gtcttcgcaa
    16381 aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa atgatttaaa
    16441 aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca agctaaaaca
    16501 ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga aagaacaagg
    16561 acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc aatttctttc
    16621 tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc atacaatggc
    16681 tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt gtcattattg
    16741 tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga gaaacgtttg
    16801 ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc tctgtgttac
    16861 ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag aatcctcgtt
    16921 acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt ttcggcgggt
    16981 ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc agaaacgtga
    17041 tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt tacagtctgc
    17101 tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa tattggatat
    17161 ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact ttcaaaaaga
    17221 aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta gtttcattgt
    17281 tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg gaaatccgga
    17341 ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga atcgtgtcta
    17401 tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg tccgctattg
    17461 gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc tactacatca
    17521 ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact tttgatcagc
    17581 gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc gtggttgtct
    17641 tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa gcttttacag
    17701 agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc caatttccta
    17761 gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca cgtgtgaaac
    17821 gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt ttattaagtg
    17881 attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt cttgcttcta
    17941 aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg atcgatggaa
    18001 tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat ctgccggatt
    18061 tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa aagatttggt
    18121 tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt gttcatttcc
    18181 ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc ctgaagatcc
    18241 ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat aagcggatgt
    18301 ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc ttcaataata
    18361 aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa gcaatcgaag
    18421 cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa acaagcgaag
    18481 agattgttat gattttgcat gaattcggct acgatttcat taagatgggg gaagaagctt
    18541 atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact cgtgctgtct
    18601 tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca ttttctactg
    18661 agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga aaggaaaaag
    18721 ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt gcagttgtca
    18781 aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac acgaacaata
    18841 aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct ggcagtatgg
    18901 atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa tggttcaatt
    18961 tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta caggaaagga
    19021 ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga ttaagagaat
    19081 ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct agagatagtt
    19141 ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt gagcagacac
    19201 aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa ctgcataccg
    19261 atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag ttcatgcttc
    19321 tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa tcctgtttgg
    19381 accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg tttcttatgc
    19441 ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa ttgagaatgt
    19501 cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca cgttgtcacc
    19561 aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat cagttgtgcg
    19621 gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac cttcttcttt
    19681 tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac ctggtttagc
    19741 aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca cgcctacgtt
    19801 atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg ttacaactgc
    19861 tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac gaacagcacc
    19921 acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga cgtcttccac
    19981 tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt catctactgt
    20041 atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg cttttaatgc
    20101 ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca ataactcgcg
    20161 aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa ctttgttcaa
    20221 gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt cacgtgtttg
    20281 aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt gtgctgcccc
    20341 tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg cgtagtggcg
    20401 tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt cacgttcttc
    20461 tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt tgcctaatac
    20521 tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa tcgtaccaat
    20581 gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca aacctcctaa
    20641 ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct ttttttgact
    20701 aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc ttattatacg
    20761 tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa aaaagtacct
    20821 tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt aactttcttt
    20881 tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca acaactttca
    20941 atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca tgaaaaaatt
    21001 aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag cttatcatta
    21061 tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa tgtcagctac
    21121 ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg cttataataa
    21181 agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat taaagatgaa
    21241 tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg aagaaataaa
    21301 ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa tggtcttaaa
    21361 attttattag ataaaaagag gctgggacag aagcgtttaa ctccaagaaa taagaaggag
    21421 ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt
    21481 ttgtctcacg ctcttttttt gttatattcg tcttttccga tccttctctt gacacaattc
    21541 atttaactga tcactggtac ttgaacacct aatattctag tcatttttat aaacgattca
    21601 atacttcatc cacatttttg atcatcacgg atatcgtacc atccggatta ttcacaaatt
    21661 caatcaaatc tgggttgcga tatacatcta ctggtacgat cagttcgatc ccgtttgata
    21721 gcttgaattt ttgttttcca aattttttct cagaaatttc ttgtacttct tttacaggag
    21781 gtgcttctgg gacaaatcct tgctcttgta cttcttcttt aaaagccatt tttgccgtga
    21841 tgttttcctt gaagactttt tccgcaatca tctcatgatc caaccgacct ttttcttcta
    21901 tcgtatcata gacagcttct ttcaaatcag ccatgatatc aaattcttct gtctcaaatt
    21961 tttttccaag atgcttggct actttttgga tcacttttac attttcttct aaagaaggtg
    22021 caggaacact ttcgatcact cttccagaaa aataaagctc ttttttgcca gaaaactcat
    22081 attttttttc aattaattcg taggtccaat cagtcaaatt cacagcgaat gcttcatctg
    22141 ctttttgtgt tttcgcccct agaattgctc gattcaaaat caagcggttg tcgatccctg
    22201 cttcgtctgc ttcaacaaag tgagtgtaag cctcacgata attgactttc aataaagata
    22261 agtacatcac tgtatctaat tcatacaaga caacaaatac atctgcacta ggtgcttctt
    22321 cactttcttg gtagatatcg aaccaccgct gaacgattgc ttcacttgct tccacaaaat
    22381 tttcttgagc gattttaagt gattcaacga tcatgctttc ttctgctagt tgtccagttt
    22441 ttgtttgagc agaagagatt ttttggatct tcttttgaag aaaatcacga atatattctt
    22501 ttgtcagatc cagctctttt tgtgaaaaga ctggagaacc agtttctctg tcaataatat
    22561 gcagaattgc tttttttaaa tagatatcca ttttctcatt cccttctgtc ttagtttacg
    22621 gaaaaaaaat caaaaatacc agaatcaaaa tcattttctt ttaaacaaaa atttcaagag
    22681 tagaaacaaa atggattctt tttttcgaaa aatgcgctac acttatagtt gaacgaataa
    22741 aaaaggagct gaaagtatga attcattaac aagcacttat cgtctatcaa atggttacga
    22801 aatcccggta gtaggattcg gcacatggca aacacctgat ggggatgtcg ctgtttcctc
    22861 tgttaaagaa gcgctggctg caggttatcg ccatatcgat actgcccaag gatacaagaa
    22921 tgaagaaagc gtcggccaag cgattaagga aagtggaatt cctagagaag agatattctt
    22981 gacaacaaaa ttatggaatg caaaccacag ctatgaactt gtgatgtctt cttttgaaga
    23041 atcattgaag aagctccaaa cagattattt agatctgttt ttgatccact ggccaaaccc
    23101 tgttgctttt cgtgataatt gggaacaagc aaatgcagat acatggcgag catttgaaga
    23161 actgtatgaa gcaggtaaaa tcaaagcaat cggtgtgagc aatttcttac cccaccatct
    23221 agatacacta gcaaaaacag caaaaattat gccgatggtc aatcaagttt tccttgcacc
    23281 aggcgaatta cagacaactg tagttgagta tgcaaaaaaa catgatatga tcctcgaagc
    23341 atatagtcca ttaggcactg gtaaaatctt cgatgtgcct gaaatgaaac aaatcgctga
    23401 agcacatgac aagacgattg cacaagttgc tttgcgttgg tcgctgcaac acgaattcct
    23461 tcctcttcct aagtcagtga caccaagcag aatcaaagaa aatactgaat tatttgattt
    23521 tgaattaaca gaagaagaaa tgaaacaaat cgatcagtta gatggtgtag tcggcaaagc
    23581 aaaagaccca gatactaccc aattttaagt cgaaaggtga ttttatggat ccaatcgaac
    23641 gcttgaattc tctctcggaa gaagttaccc agacttttca ttccgatttt gtctttttga
    23701 tcgatgcaga gaaaatccaa cattttcctg caagaaactg gacacatgat caaatcatcg
    23761 aagaactgaa aaaacggttc gatcattcgc taatggtcaa accctggcac gaacatgaag
    23821 tcatttattc acctgaacta cctgtttttg ccttgatacc gaaaaaatag aaagaatgga
    23881 acggattatg tatatataca taatccgttc cattttttat ttttctttca aaaaacggga
    23941 aaatacgcaa atttttctgt tgtaatttgc gtatacgcaa gtttttttgc attactcctt
    24001 ttcatttggc tgtttttttc ttataatcga aagggtatga gcgaaggaga gaacatatga
    24061 agaaaaaaca gaaacaaaaa ttattaaatc aattccgtcc ttcattggat ggtgtaagag
    24121 gacaattatt ccgttctcta gaagaagaga gcttgcgacg ttatggtttg ccgttacaag
    24181 tcatgcttga tcccaaaaac cgtcaaatct taacgatcgg attagctggg aaaacaaatg
    24241 aagatacacg gatcaacgtg ccaatcgatg acaacttcaa tactgtatta aagagaattc
    24301 gttcaggaga aaacgggatt ttcgaacgat tccgtgacaa tcttcttatt gaaatcgtgt
    24361 cttactggaa tgatcaacga ttgaaaaacg atgaaccaac tgcgcaagct gttgcgacac
    24421 cagttacaga agaaacagtc agtgtgccag aagaaaaaac ggaaaaacca aaagcggaca
    24481 caactgaaaa tacacgctca actgatacga atgccgcttt aagttttgct gatttttcaa
    24541 aagccgttgc tgagtatcct aaattttaca cagaacaaaa tgaacatgaa gtagttatta
    24601 atgaaaaaaa tgctgatgaa cctcgtaaat tagcaactat ttctatgact actgaaaatg
    24661 agttcgttat cgaaaaagca ctcgaacgga aatacaaagt caaattgact ttgattcctt
    24721 tgatcgaaca atttgcagcg acagcgatta ctgatcgata aaaattctat ttgtcataaa
    24781 aggctgggat ttaaaatccc agccttttta gtcatataat aatttctaca gcaagtaaaa
    24841 agaaattcct tctaacatca ggctcaaata tcagcttgac ctgtccaaca aatcacctat
    24901 gatcctacaa tgttaggcga cttctcttct acctgaagat atcttggagt tgcctgattt
    24961 cttaagatgc ct
//
LOCUS       DAZUJO010000034        24257 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000034 DAZUJO010000000
VERSION     DAZUJO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.034"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..995
                     /locus_tag="ACG8OL_001497"
     CDS             138..995
                     /locus_tag="ACG8OL_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS4817490.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1110..2030
                     /gene="pstC"
                     /locus_tag="ACG8OL_001498"
     CDS             1110..2030
                     /gene="pstC"
                     /locus_tag="ACG8OL_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS4817491.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2030..2914
                     /gene="pstA"
                     /locus_tag="ACG8OL_001499"
     CDS             2030..2914
                     /gene="pstA"
                     /locus_tag="ACG8OL_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS4817492.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2929..3735
                     /gene="pstB"
                     /locus_tag="ACG8OL_001500"
     CDS             2929..3735
                     /gene="pstB"
                     /locus_tag="ACG8OL_001500"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4817493.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3751..4509
                     /gene="pstB"
                     /locus_tag="ACG8OL_001501"
     CDS             3751..4509
                     /gene="pstB"
                     /locus_tag="ACG8OL_001501"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS4817494.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4522..5199
                     /gene="phoU"
                     /locus_tag="ACG8OL_001502"
     CDS             4522..5199
                     /gene="phoU"
                     /locus_tag="ACG8OL_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS4817495.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5437..5736
                     /locus_tag="ACG8OL_001503"
     CDS             5437..5736
                     /locus_tag="ACG8OL_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817496.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5777..6469
                     /locus_tag="ACG8OL_001504"
     CDS             5777..6469
                     /locus_tag="ACG8OL_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4817497.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6466..7233
                     /locus_tag="ACG8OL_001505"
     CDS             6466..7233
                     /locus_tag="ACG8OL_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS4817498.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="ACG8OL_001506"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="ACG8OL_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS4817499.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9198..9518
                     /locus_tag="ACG8OL_001507"
     CDS             9198..9518
                     /locus_tag="ACG8OL_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS4817500.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9518..9868
                     /locus_tag="ACG8OL_001508"
     CDS             9518..9868
                     /locus_tag="ACG8OL_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS4817501.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /gene="hprK"
                     /locus_tag="ACG8OL_001509"
     CDS             10035..10973
                     /gene="hprK"
                     /locus_tag="ACG8OL_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS4817502.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /gene="lgt"
                     /locus_tag="ACG8OL_001510"
     CDS             10988..11818
                     /gene="lgt"
                     /locus_tag="ACG8OL_001510"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4817503.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="ACG8OL_001511"
     CDS             11862..12887
                     /locus_tag="ACG8OL_001511"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4817504.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="ACG8OL_001512"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="ACG8OL_001512"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS4817505.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="ACG8OL_001513"
     CDS             13965..14405
                     /locus_tag="ACG8OL_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS4817506.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="ACG8OL_001514"
     CDS             14408..15010
                     /locus_tag="ACG8OL_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS4817507.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="ACG8OL_001515"
     CDS             complement(15078..16184)
                     /locus_tag="ACG8OL_001515"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS4817508.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG8OL_001516"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="ACG8OL_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS4817509.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17731..20076)
                     /locus_tag="ACG8OL_001517"
     CDS             complement(17731..20076)
                     /locus_tag="ACG8OL_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS4817510.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20355..21257
                     /locus_tag="ACG8OL_001518"
     CDS             20355..21257
                     /locus_tag="ACG8OL_001518"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS4817511.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21470..23245
                     /locus_tag="ACG8OL_001519"
     CDS             21470..23245
                     /locus_tag="ACG8OL_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS4817512.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23502..>24257
                     /locus_tag="ACG8OL_001520"
     CDS             23502..>24257
                     /locus_tag="ACG8OL_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4817513.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
BASE COUNT         7748 a         4334 c         5089 g         7086 t
ORIGIN      
        1 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
       61 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
      121 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcca
      181 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
      241 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      301 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      361 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      421 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      481 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      541 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      601 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      661 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      721 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      781 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      841 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      901 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      961 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
     1021 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
     1081 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
     1141 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1201 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1261 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1321 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1381 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1441 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1501 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1561 gaagtatttt tggcgggaca gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1621 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1681 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1741 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1801 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggactta
     1861 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1921 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1981 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     2041 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     2101 tttagcagct ttactacttt atatcttagt tcggggaata ccgcatattt catggtcctt
     2161 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2221 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2281 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2341 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2401 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2461 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2521 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2581 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2641 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2701 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2761 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2821 cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2881 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2941 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     3001 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     3061 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     3121 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3181 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3241 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3301 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3361 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3421 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3481 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3541 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3601 caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt aatggaatat
     3661 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3721 ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatttc atctaaagat
     3781 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcaatctaag tttcaatcaa
     3841 ggggaaatca ctgcgatgat cggtccttct ggttgcggga aatccactta tctgcgttct
     3901 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3961 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     4021 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     4081 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     4141 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4201 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4261 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4321 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4381 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaacgat
     4441 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4501 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4561 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4621 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4681 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4741 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4801 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4861 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4921 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4981 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     5041 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     5101 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     5161 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5221 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5281 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5341 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5401 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5461 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat
     5521 gaagtacctg tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca
     5581 ggaagactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5641 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5701 ggagatgaat ggcagctagt agcaaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa ggggttatga caaaattttg tttaatcctt tttttttgtc
    17581 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    17641 gatgctgcat tatgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    17701 cagtccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttggt
    17761 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    17821 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    17881 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    17941 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    18001 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    18061 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    18121 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    18181 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    18241 atctttgaat ggtgtagtga tttttgcatt gatcacactg cgcatcatgt cattcatgat
    18301 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    18361 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    18421 taaagcctga aatccattgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    18481 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    18541 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    18601 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    18661 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    18721 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    18781 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    18841 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    18901 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttctacgcc
    18961 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    19021 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    19081 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    19141 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    19201 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    19261 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcacc
    19321 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    19381 ttgcggcagc gtcacatctt ttgcatctac accaaagatt cctcttgcag cttcctggac
    19441 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    19501 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    19561 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    19621 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    19681 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    19741 tttcaataaa ggtgaaattt tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    19801 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    19861 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    19921 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    19981 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    20041 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    20101 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    20161 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc ttttttataa aagagttctt
    20221 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    20281 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    20341 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    20401 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    20461 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    20521 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    20581 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    20641 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    20701 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    20761 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    20821 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    20881 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    20941 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    21001 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    21061 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    21121 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    21181 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
    21241 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    21301 cttgtccttt tttgtttaag aaacaaagga gtgctttttg cagtcttttt catcatgcat
    21361 cttctccccc ctctttttaa atttagacaa acagggtata ctggtagaaa agtaaaaaaa
    21421 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    21481 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    21541 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    21601 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    21661 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    21721 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
    21781 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
    21841 aagtttctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    21901 aacgtatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
    21961 tatttattac cgtaaatata gttccagcaa aaaaatgctg ccgtggtttt tagggctagc
    22021 tgtttctttt cctttctttt ggttcattcg cgaagattcc atctggcttc ttccttttac
    22081 tgtagtcgcg ttaggcatca gtttcgtcca gatcttgcta ggacaagact tttctttcaa
    22141 agaagcaaag aagttaaaag aacgaccatt tctcctttcg aaacaagaat ggctgaaact
    22201 ttcattgttt ttactccctc tagtcggttt ctccatcgtc tatcagtgga tcgaatataa
    22261 aaacgagaca acatatagta ttgccgccat caatgaccgt tcgagtggcg aatttggacg
    22321 tttgatgaaa cagctgatcc gaatagatga cgaaacgaac ttaaataaag aaaacagtga
    22381 gatatgggtc tcgcatgctg cattaaataa agcattagac gcatctccta catttcatgc
    22441 tgtttctact cgtattgacg aattgtatac gactcatgcg tggacccaag gtggaaaagt
    22501 aaaagaatta ccaggagata tcattttttg ggcattgcga gatgtggtaa gcgaatctgg
    22561 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca atcaagaatt
    22621 agaagaagcc tatcaaaagg gaactatcaa aaagaaaaaa gaattctatc tgatgggatc
    22681 aggggatgga aagacgatga atgatctgcc aatcgtattt gcttttctga aatcaggata
    22741 tgcctatgca tggcgataca aagatttcca tcaagggggg aattatagtt tcggtacgca
    22801 agaacaagtc gaacaggccc aagaattatt gaatcttcca ctaatggata attggatcga
    22861 tagcagtgat ccgagaacac ctgatttcca gttgacaaaa acagcaaaag tcgcgaatgt
    22921 catgataaaa atttaccaag ttctggccat tcccgtgctg attgcttcaa tagcagggat
    22981 attacttatg atactaggaa ccgcatttgc aaaagctgat cgtgcctatt atagttcctc
    23041 tttgattgta gtcaccggat taatactgac acaattggtt tttcttttcg gcgtctcgtg
    23101 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
    23161 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattgaatct
    23221 gaaaaaagct ttatcttcca aataaaaaca tactaagatt agaggagaac atcattgacg
    23281 aatgatgttc tcctctattt tctattttta gattatagtg aagagtgaaa gaaagttcga
    23341 acaaagcctt gaggactatg agtccctatc aaaaaccgaa cttctttact gtcattttag
    23401 aatcaggttt acgtatgttg gggccatgag cccgtgtata ggaaattgga atcgaaatga
    23461 agcagtaaag ttctttcaaa acttaaaaag gaggaaaaca aatgccttac attcttgcat
    23521 tagatgtttc aatgggacac agctacagtg tcctatacaa agacgacacc tgcttatttg
    23581 aagacgagat cgaacacact caaagaggat ttcatgcatt actcgaagag atccagcact
    23641 tgcctgaacg tccattgatt gtttttgaag caacggggat ttattccaga ccagtcgaaa
    23701 agttctttca agatcatcac ttttcttact gtttgttaaa cccgttagaa gcgaaaaaac
    23761 agatggaaga aaacacctta tgcagttgga aaacagataa atcggatgcc catcggctcg
    23821 cacagactca tcttaaaaat caacgtcagc caaaagaagt acaaagcaat ttttacttag
    23881 agatgcgcga tttagcccgt ttttatcaag aaattgagaa gaaaatcact cgtctgcgca
    23941 tggatttaca caactgcctt cagttgactt ttccagagct tgaacagttc tttttaaaca
    24001 ggctgacccc ttacgcattg atattgatcc gtctttttcc ccatccagat tttgtattgg
    24061 catctactcg gacaaaaata aaaaataaac tgatcaacga aacacggaaa aaaatctccg
    24121 cgaatcgtgc cgagcaaaag gcagatcaaa tcatccacta tgcacagtgt gcctatccag
    24181 ctgttgaaaa agacagtatc cactgtcaaa aaacgatcta ttacgctgaa cttctccaag
    24241 atttactcga acaaaaa
//
LOCUS       DAZUJO010000035        24249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000035 DAZUJO010000000
VERSION     DAZUJO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.035"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACG8OL_001521"
     CDS             complement(42..1337)
                     /gene="purB"
                     /locus_tag="ACG8OL_001521"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS4817514.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACG8OL_001522"
     CDS             complement(1350..2474)
                     /gene="purK"
                     /locus_tag="ACG8OL_001522"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS4817515.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACG8OL_001523"
     CDS             complement(2467..2955)
                     /gene="purE"
                     /locus_tag="ACG8OL_001523"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS4817516.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3371..4678)
                     /locus_tag="ACG8OL_001524"
     CDS             complement(3371..4678)
                     /locus_tag="ACG8OL_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS4817517.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4681..5262)
                     /locus_tag="ACG8OL_001525"
     CDS             complement(4681..5262)
                     /locus_tag="ACG8OL_001525"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS4817518.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5378..5475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5834..7126)
                     /locus_tag="ACG8OL_001526"
     CDS             complement(5834..7126)
                     /locus_tag="ACG8OL_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4817519.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7123..7746)
                     /locus_tag="ACG8OL_001527"
     CDS             complement(7123..7746)
                     /locus_tag="ACG8OL_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817520.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7749..8585)
                     /locus_tag="ACG8OL_001528"
     CDS             complement(7749..8585)
                     /locus_tag="ACG8OL_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817521.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8602..9489)
                     /locus_tag="ACG8OL_001529"
     CDS             complement(8602..9489)
                     /locus_tag="ACG8OL_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817522.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9505..10788)
                     /locus_tag="ACG8OL_001530"
     CDS             complement(9505..10788)
                     /locus_tag="ACG8OL_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4817523.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11471..12154)
                     /locus_tag="ACG8OL_001531"
     CDS             complement(11471..12154)
                     /locus_tag="ACG8OL_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS4817524.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12172..12789)
                     /locus_tag="ACG8OL_001532"
     CDS             complement(12172..12789)
                     /locus_tag="ACG8OL_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4817525.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12799..13623)
                     /locus_tag="ACG8OL_001533"
     CDS             complement(12799..13623)
                     /locus_tag="ACG8OL_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS4817526.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13616..14176)
                     /locus_tag="ACG8OL_001534"
     CDS             complement(13616..14176)
                     /locus_tag="ACG8OL_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817527.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14350..15306
                     /locus_tag="ACG8OL_001535"
     CDS             14350..15306
                     /locus_tag="ACG8OL_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4817528.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15611..16567)
                     /locus_tag="ACG8OL_001536"
     CDS             complement(15611..16567)
                     /locus_tag="ACG8OL_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS4817529.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16671..17273
                     /locus_tag="ACG8OL_001537"
     CDS             16671..17273
                     /locus_tag="ACG8OL_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4817530.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17361..18860)
                     /locus_tag="ACG8OL_001538"
     CDS             complement(17361..18860)
                     /locus_tag="ACG8OL_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS4817531.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18876..19400)
                     /locus_tag="ACG8OL_001539"
     CDS             complement(18876..19400)
                     /locus_tag="ACG8OL_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS4817532.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19698..20447
                     /locus_tag="ACG8OL_001540"
     CDS             19698..20447
                     /locus_tag="ACG8OL_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4817533.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20438..21223
                     /locus_tag="ACG8OL_001541"
     CDS             20438..21223
                     /locus_tag="ACG8OL_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4817534.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21192..21908
                     /locus_tag="ACG8OL_001542"
     CDS             21192..21908
                     /locus_tag="ACG8OL_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4817535.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22010..23029)
                     /locus_tag="ACG8OL_001543"
     CDS             complement(22010..23029)
                     /locus_tag="ACG8OL_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS4817536.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23048..24049)
                     /locus_tag="ACG8OL_001544"
     CDS             complement(23048..24049)
                     /locus_tag="ACG8OL_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS4817537.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7187 a         5222 c         4223 g         7617 t
ORIGIN      
        1 ttgctattct attttaattg aattttgcaa ctcactcttc tttattccaa acctactcgt
       61 ttgaatatca cgtcaacgtt tttcaaatga tgatgataat caaaggcatc gtctatttct
      121 tctttagata ataccgatgt gatggttgga tcttcttcta acagcgggcg gaactgcact
      181 tggttgtccc aagcgtacgc agtttttggc tgaacaagat cataagcttc ttctcttgtc
      241 attcccttat cgatcaattt cagcataacg cgctggctat aaatcaaacc aaatgttgca
      301 gacatattcc gcttcatgtt ttcaggaaag accgttagat ttttaacgat attgccaaaa
      361 cgatttaaca tgtaattcaa taaaatcgtg gtatcaggca agatgattcg ttctgctgaa
      421 gaatgagaaa tatctctttc atgccataat gtgacatttt cataggcagt gaccatgtgt
      481 ccgcgcacca ctctggctag accagccata ttttctgaac cgatcggatt ccttttgtgc
      541 ggcatagcag aagaaccttt ttgacctttc gcaaaaaatt cttctacttc acgtgtttcg
      601 gatttttgca atccgcgtat ctctgttgca aaacgttcaa tacttgtagc aataagcgcc
      661 atcgctgaga aatattcagc atgtagatcc cgagggagaa cttgagtaga gatttcttgt
      721 ggacggatcc ccaattgttt acatacatac tcttcaataa atggcgggat atttgcgaac
      781 gttcccactg ctcctgagat ttttcctgct tctactcctt ttgccgcatg ttcaaatcgt
      841 tcgatgttcc gtttcatttc tgaataccat gtggcaagtt tcaatccaaa agtagttggt
      901 tcagcatgga caccgtgtgt ccgacccatc atcactgtat atttgtgttc ttttgctttt
      961 tcgccaatga tttccgtaaa acgtttcaag tcttcccgta aaatatcatt tgcttgcttg
     1021 atgagatagc cataagcagt atctacgaca tccgtacttg ttagaccata atggacccat
     1081 tttctttctt ctcccaatgt ttcagaaacc gctcgtgtaa acgcaacgac atcatggcgt
     1141 gttaattttt cgatttccaa gatccgatcg atatcaaatg atgcatgctc acgaattttt
     1201 ttcacatctt cttgcgggat ttcaccaagt tctgcccaag cttcatctgc taaaatctcg
     1261 acttcgagcc acgcacggta tttgttctct tccgtccaga tattccccat ttcagggcgt
     1321 gtataacgtt ctaacatgtt ttttctcctc taatcccaaa tattcgtttg tttgatttct
     1381 tccagcgttt gctggatgtc ttctgtcaag atagtgatat gtcccatctt gcgacctttt
     1441 tttgcttgtt tttttccata gaaatggaat tgccagtctg gcttattctc aatcaagcga
     1501 tagctttcca gcagttgttc tcccaaaaca ttgaccatta ctgcattgct tatcaaacgt
     1561 actttttctc ctaatggcca accacaaata cctcgaatat gggcatcgaa ctgactcata
     1621 gaacaagctt caatagaata atgtccagaa ttgtgaggac gaggagctaa ttcattcaca
     1681 tatagcccgc ctgtttttgt taaaaacatt tctaccccta aaacacctga aaggctcact
     1741 gcttctgcaa tcacacgggc aatacgctca gcctcctcga tcacatcctg atcaatcgcg
     1801 gctggagcaa tcgtttcatg aagaatattg ttgtggtgga tgttttccac gaccggaaaa
     1861 gtcgtgaaat ctccttgacc atttcctgcc accatgatcg aaatctcttt ttcaaacggg
     1921 atccatgctt ctaaaacaca ggttccttct cttaaaaggt ccatggcagg agctagatcc
     1981 gcccgacttt tcaaaacata ctgccccttg ccatcatagc cgcctcgagt ggttttcaaa
     2041 acacaaggat agccgatccc gtcaatcgca tcttggatat ccgtcggact gacgatcgtt
     2101 gcataaggag caatcacgat gttattcgtt tccaaaaaag atttttctaa caggcggtct
     2161 tgcgtaatcg caagcaagtc tgtcccttga ggaataaatg acatgggtaa aatcgcattt
     2221 aacgcctcta cactgacgtt ttcgaattca taggtaatga catctgttct tctggcaagc
     2281 tctttcagcg caaaggtgtc atcatagaca ctttcaatgt gccaatctgc aacttgtgcg
     2341 gctggacaat cgctcacagg atccagcaca cctactttaa agcccatttc ttttgcacta
     2401 atcgccatca tacgacctaa ttgaccgcca ccgacgatcc cgatcgttga accaggcaac
     2461 aaaggtttaa ccaagttgat cactactttc tataacagtt ttagccaaca ttgaacgtct
     2521 ttcagccaat ttagcctcca attctaaatc atacatggaa agcatctgta tcgcaagtag
     2581 tccagcatta attgctcccg cttttccgat tgctgtagta gctacaggta ctcctccagg
     2641 catttggaca atcgaaagaa gcgaatccaa accatttaat gttcgtgatt ggaccggtac
     2701 accgataacc ggcaaagttg tttttgatgc gaccataccc ggcagatgcg cggctccccc
     2761 tgctcctgca ataatgattt tgattccttt ttttcgagcg ttttgtgcat attcaaacat
     2821 caaatctggg gttcgatgag cagaaacgac ctttttttca taagacacac cgaattcatc
     2881 caatatttga catgcttctt tcattgtttc ccaatctgaa gtactcccca taatcacaga
     2941 gatttttgct gtcattgttt ttcctcccag tttttctaca ttcatatttt aacaatcact
     3001 ttttttgatg tcaaagaaat ttcgaatgtt aacaaagaaa aacattttat tgttcgtgtt
     3061 tattaaagat aaaaaaaagg aatgtgacaa aagttaggtc acattccttt ttatggataa
     3121 acggtgttct cctgccaact cctcggtcac aaggaacaat attcaacaaa catgagaagc
     3181 ttttttttga aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttcct
     3241 tcaacggtgt tcaatcagct gaccctcggt attagctcaa acaaacggca aaatacgagg
     3301 aactattttt ccgtttgttt ctctaacaat caggtcaaaa cgctgatttc acaacctttc
     3361 ttttgctcta ttacttcttg aaggcttctt ccaatatttc ttcatctttt ttcaatccat
     3421 taaagatcaa attcaatatg atagccgtaa tgctactcat tacgatgcca tttccagtga
     3481 acatttgcaa tgtttgcggc atttgactga ataatgtcgg cgtaacattg aaacccagcc
     3541 caaatccaac agaaacagca acaatcaata gatttttatc attgctgaaa tcaacttcca
     3601 acagcatacg gattccttgg actgccacca tcccgaacat gactaacatt cctccaccaa
     3661 gtaccggctc agggatgatt tgtgctaacg caccgatttt agggaataat cctaaaacaa
     3721 tcaagaagaa tgcagagaaa tagatgggtt ttcttgtttt gattcccgaa agttgaacca
     3781 atccgacatt ttgagaaaag ccagtataag ggaatgtatt gaaaataccg cctaagataa
     3841 cggcaagtcc ttctgcacga tatccctttt tcaattcttc ttctccaaca tttttcccag
     3901 taatgtcacc aagtgcaaaa tatacacctg ttgattctac catgctgacc atggaaatga
     3961 tgatcattag taaaatcgac catacgtcga aagttggttt accaaaataa aagggttgtg
     4021 gaacatggaa caacggcgca tctccaatcg cgctcagatc aatcattccc atgaaagcag
     4081 ctaagatgct tcctccgact aaaccaatca ataccgcgat tgaacgaatg aacccttttg
     4141 ccagcatttg cacgcctaca attaggagaa tcgtcacaaa tgcaagtagc agatttgttt
     4201 tgtcaccaaa atcagctgct gttgcattac ctccacccat tttctcgatt gctacaggaa
     4261 tcaaagtcaa accaataacg gtgataaccg ttcctgttac tataggaggg aatagttttt
     4321 taattttaga aaagacacca gaaactaaca caacaaagat cccagaagcg ataatagatc
     4381 cgtagatagc tcctactcct tcattggttc cgatcaagat cagtggagct actgcttgga
     4441 tcgcacatcc taaaacgaca ggcaagccaa ttccgaaaaa tcggttaact gtcaattgca
     4501 ataatgtcgc taatccacac ataaagatat caattgaaat caaataagtc atctgttctt
     4561 gattaaagcc tagtcctgta ccgatcaaaa gaggcaccgc tactgctcct gcatacatcg
     4621 ctagcagatg ctgcagccct aaaaccgcag cttttccatt ttgtgtttct ttttccacga
     4681 ttatgcgtcc tcctcaagaa actcaacttg tccattcgtc aaggaagcaa tacgcgcaag
     4741 agaaacgact ctcaatccca tttcttcaag taactgacgt ccatcttgga aagacttttc
     4801 gatgacgatc ccgattcctt ctacttgcgc tccagcttgc tggcataatt cgatcaatcc
     4861 ttttgccgct tgtccgtttg ccaagaaatc gtcgataatc aatactttgt cttctgaaga
     4921 taaaaattta cgagaaatag aaatcgtact tgtcacttgt ttcgtaaaag aataaacaga
     4981 agctgtcaat aattcttcat ccatcgtcaa actttttgct tttctagcga agatcattgg
     5041 aacatctaat tctcttgcag caaataaagc tggtgcgatc ccagaagctt caatggtcac
     5101 aactttcgtg attccttgtt ctttaaatac ttctgcaaac cgttttccca tttgttccat
     5161 caatacaggg tctacttggt gagtgacgaa gctgtcgact tttaatacgc cctcgcctaa
     5221 tatacgtccg tctttttgaa tacgttcaac taattctttc actttatttg ctcctttatc
     5281 atttattaat accgaaataa ccgcttttgt gcaaaaacct actatgtttc tgttaaagaa
     5341 aaacaccctc cactttatca taagaaaaga agggtgaaat agccaatctt ttacttatag
     5401 tccagcattt acggtactgg ctagaaactg tcatccatat tatgacgata tataagtagg
     5461 tatgtaaaac taataatagt gtaaattcat tttcttgcaa ctgttttgca cgtgaaggca
     5521 gtttgccata tttgaataaa aaatgttgtt tcaaatattg aaaacagtct aacactactt
     5581 tctgaaactg acaacgtggt ttacttattt ttttgtaaaa tctaatgatt tttaatgcat
     5641 tattcctttt tgttcgggtt taaatagtta tttttatttt ctcctatcta aaaaagcagc
     5701 ccacataaaa agaaggcatg acaaaaagca tgtcatagcc cctttttccg aataaatagt
     5761 gttcaaaaga cagcctatcg gcaataaggc aagattttat aaactacggg aagtaatttc
     5821 cgtaaatcta ttcttattct tttaagctga caagctcgtc aatcactact gcttttcctg
     5881 aagcaataga atgatttgct gcgattcctg tcaaaatcga caaaatacca tctacgtgag
     5941 ttgctgcccg attccacgga tcttcttctt tttcttcacc aaatagatca tctaataaac
     6001 gcggatctcc tccgccatgt ccgcccgctt cttgttcgac gattacctca tatggctcac
     6061 cgaacatcgg atagacatgg atggactgaa gaacggctgc tccttcatcc tttttatctc
     6121 cacctgcatt gatgtaagac ttttcgatca ctttcatttc gatccgtcct ttgctgccat
     6181 tgaaagaaac attgaatcct tcccacggca tatacgcgtt tagcgaatac gtcaaaatcg
     6241 caccagtttc atatttgacc gttacgccta gtgtatcttc aatagagata tcatcggaaa
     6301 atacactgcg gtcacgaata tatccactgt ctttttctgc ttctaaatac atttctcgca
     6361 attcctcatt ggctgacagg tctatagcaa atggatcagc tttagctagt tcgcttccat
     6421 agcatcgact gtaaaatatt tctactccgc gtttctccgc attatgcatc ccataaaagt
     6481 ttaatccacc gaatgcaaat accttttggg gacggctgtt caaccaaaaa ttcactagat
     6541 caaaatgatg ggtagattta tgaacaagaa gcccgccgct attgtgctta tttcgatgcc
     6601 atcttctgaa ataatctgcc ccgtgttgcg tatttaacag ccattcgaag tggatagaaa
     6661 acacatcacc aatcacatca ctttctatca gttcctttat cttcgtgtga tacggtgcat
     6721 aacgataatt aaaagtgact cgcaggcttc ttcccgtctg tttttggacc gttattattt
     6781 cttgagcttt ttcctcatcg atagtcagag gtttttcgca aataacatca tatccttttt
     6841 tcattgcacg aatgatataa tcatgatgcg ttcgatctac tgacgtaata atgacttcag
     6901 ccggccgtac gtcatccagt agttgctcaa attgtgatgg catacatgtc tttacttttt
     6961 tctgtccata cttgttcaca agtatccgat ttgcataatc catccttgtt tgagatacat
     7021 cacaaaaacc gactagttca caagtttgat gatacgtaga ggcaattgcc tcataaaaaa
     7081 aacgcgctct cccaccaagt ccgacttgta catatttttt cttcatttaa agtcattctc
     7141 cttttctttt aagcaaaata ctgtgacaaa tggcagccct ggatacagta atgcagtgag
     7201 taaaaaatca tgctgtatac accagaacag tccatacaaa aggcataaat accacagaaa
     7261 ccccttgacg ttttttgaca acagccagat attccttttg atcaaataaa caagactatc
     7321 tgctttatct tctttgcaga tatcaattag cgtactaaaa aaactatggg atacaaaaag
     7381 caacagacac aatagaaaag gaatcagata ccaagctcca atctttccca ctaataaata
     7441 ccaatcaact gcaaaaataa tgacaaacag ctgaaacaac gtaatgatga aacacatccg
     7501 tttgattttt cctttcctcc aacatacctt tttttctgcc aagtaatgga cagatagata
     7561 ataagaagga tacatcatca aaaaataaat cagatagaca acacgagcag acagcactaa
     7621 tcctaaagtg aacaaacatc ctagaactaa agcgaaaaga gcattactga taagactggt
     7681 taaaaaaatc cgacagacat agtagttgag cttgtaaaga aacatctctt ctattggctt
     7741 tttcatattc agcccttcag accgcttgta ctgatcccct ggacaagata tttattgaag
     7801 aataagaaaa tcagaaaaac cggtagcagc gacaacgtag acatcgcaaa catactcgcg
     7861 taagtcgtac tagatccagc gtctgcaaat aatttgatgg caatcgatac cgtgtaagat
     7921 ctcggcttgt ttaaataaat caatggtccc atgaagtcgt cccatttcca ataaaactgg
     7981 atgatgatcg tcgtgatgat cgaaggtttc agcaaaggaa aaataatttt ggtaaagaca
     8041 ctatacttgc tgcatccgtc aataatcgct gattcatcta actctttggg gatgctgacc
     8101 atgaattgca tgatctggta aatgaaaaat gcttgtccaa aaaaatgtgg taaaatcagc
     8161 ggcacatacg tcccaaccaa attcagccga ttgtaaataa taaactgcgg aatcaaaatc
     8221 acttgtgcag gaatcatcat cgttactaac atgacgacga accagaatct ttttcctttg
     8281 aaatcgattc tcgataatgc gtaagcaata caagtcgagc tcagaactgt ccctatcgtc
     8341 gaaactgcag agatgacaaa cgaattccta aagaaagtaa aaaatgaaat tccagcaaac
     8401 cccgcccatc cttttgcgta attttcccat tgaaaagtct ctggaatcaa attcagcgaa
     8461 ccactcataa tctcttgatc cgttttaaaa gaacctgcga tcatccataa gaccggataa
     8521 atcatgacta gacctaatag caaaataaaa ccgtattgaa accatttcgt tactgtatta
     8581 ttcatttctt cctcctctca ttcacacaac gacccatttt tttgatgatt tgaaaatcag
     8641 aaaggtgaca gctcccataa tcaacagcat gatccacgaa atagcactag cataccccat
     8701 agaattaaaa gaaaaagctt ggttatacac ataaagagca taaaagttcg tggcatcatt
     8761 cggaccacct tgggtaatga taaatgcctg cgtaaaagtc ataaacgccg cgattgtctg
     8821 cataaccaag ttatacaaaa tgatcggcga aagaagaggt aacgtgatct tgtaaaacgt
     8881 ctgccatttg ttcgctccat cgatctctgc cgcttcataa tacgagcctg ggatctcttt
     8941 gatacctgca gcaaagatga tcattgccga accgaattgc caagcagaca tcaaaacaag
     9001 agggatcatg accagttttt gatcgctaaa ccaggaaagc gcaggtgcgc ctaatcgttc
     9061 caggaaaata ttcactaacc cgtttttaga aaacaactcc cgccaaacta gagcgataga
     9121 aatactgcca ccgatcaaac taggcacata atacaaagaa cgaaagaaag tcgttccttt
     9181 gatattcctc gttaaaagat aagcaatcaa tagaccaaag atcagtttca caggtacaga
     9241 tgcaaataca tagagaaaag tgactcggat ggagttcaaa aaacgggcat cactcagcat
     9301 ctttatataa ttatccaagc cgatccatga gactggattt cccaaacgat aatcggtgaa
     9361 cgaataataa agtgacaaga tcatcggtat cactgttatc aatagaaatc ctaggatgaa
     9421 tggagaagca aatacgtaac cgaccacatt gtcttgatcc aagaaccgtt ttagtttttc
     9481 ttttttcatg ccttcctcct gctgctatcc tagaatgttt gacgcaaact cataaagact
     9541 ctctgctgct tcttctgctg ttacttcttc ataaatgact ttttcaacta aaagattgta
     9601 ttggtcttga atttccggat tcaaagcact atcaaagaca ttcacttcgc cgttgtccat
     9661 atcggaaatc atttctaaat aatcaaatgt cgcttgcaaa gttgcatcat ctgcacgagc
     9721 gagctgttct cgaatattcg acatgattgg tacaccacgt tctcctttta aaatttcatt
     9781 ggcttcttca ctgttttgaa agaaatcaat aaacttagca gcttcttctt tatgggctga
     9841 gctttttgcc atactcagca tttgcgaaga acgtaaaggg ataccagatt ctccatctgc
     9901 ttttctgcga ggtggattaa tcaactgaat ctccatatct tcaggagccc ctgacaatat
     9961 ttctgtcaac tggttacttg atagaaacac cattgccgct tctctatccc taaccgcctg
    10021 aaaagacaga tccgtcgtac ttgcagctcc accacctggt tctggatggg cccctttgtc
    10081 taccagattt ttacgcagct ggaaaaaatc tgttagatag tctggatttt caaatcccaa
    10141 gctgttagat agatcttccc gattataaaa attcaatcct ttctcaactt gtggtatttg
    10201 aataatacag ccagctataa aatcttcgaa tcttgaaata gcatagattc ctaattcttc
    10261 agtgatttga tagctgattt gttcaaattc atcccatgtc caatttgaag ctggttcagg
    10321 gatatttaat tgttcaaata tatttttatt gtaagcaatc gcaaacgaat tcaccccgtt
    10381 agacagtcca actaattgcc cgtcgtaagt cgtcgttgct aaataggatt catctgcatt
    10441 agaaatatca attatccctt gctccacata ttcatctaat ggttcaatct gattgatgta
    10501 ttgaggaaat tgattgttca gttggaaaat gtcccagata tcatctgatg ctgctagtgt
    10561 acctaatttt gtgtaatatc cttcataatc ataaaattca taatcgatcg tcacatgggg
    10621 atttttttct tcataaagct cgattacttt tactgttgca tcgtgtcgga cttgcgaacc
    10681 ccaccaagcc atgttcagcg tgattttttt gtcatcttct tgcccacaag ccgtcaagaa
    10741 gaataacata acacttaaag ctaagatgat tctcattctt ttcttcattc tctgcacctc
    10801 tacttccatt gttatttttc aatatactgt atttttatac agaacatatt tattaaacga
    10861 tctttttcaa ggggaataaa aaccaagact tgccttatcg ttctgcctag gatgaacaac
    10921 tcaatacttt caagaaaaat tagatagtgc tgattcttct ttcatcgcta aaaacacttc
    10981 ttgcttcaat gatgggagga taattacgca aagaaaaaag gatatcagac aactgcccaa
    11041 tatccttgac atagctaaat aagcatgctt cttcgtgtgc gctcttccct aaaaaacaga
    11101 aaaataacta gtaattctgt atgaaattag gtacaatata cacgtcgatg cacaagcatt
    11161 gactatggca acttgattgg tcagttcccg ctgacttttc aagccccgac aatttagctg
    11221 ctactaaatt gtcgggtgcc ttttttagtt tttattcgtt tttcattcta tagaaagcgc
    11281 ttcaataaat caagacattt ttttgttaga atgctaataa taacaaaagg ttttaactct
    11341 tcttatgtac attacaggta aaaaaacaaa aaaatttctc ttttttttga attctgaaca
    11401 aatagtactg gagtacaaaa gacgaggaag agccactatc tgctcttcct cgtcttttga
    11461 ttctcacctg ttattcatga ttctttttct cagtttcccg atatttttcc attttacgac
    11521 taaaaagctc tccgctactt ggtggcgtat atgtgatggc atttgctcct gcttgaatgg
    11581 tctcagcaat actttcttcc gttggaccac ctgtcgcaat gataggaagt tcaggatatt
    11641 ttttacgaaa atggcgtact gcttctgcag tttctttacc tgcactgata ttgataatcg
    11701 aaataccagc tgctaacttt tcatctattt ctgtatattt cgacgttacc gtactgataa
    11761 ctggaatatc aactactcga caaacttctt ttactgtctc taaaggcgta ggaccgttca
    11821 aaacgacccc taacgaacct tgcgcttctg cgaacatgct catgtaggaa gaacgagcac
    11881 cttgtgtcaa accaccacct acaccggaga aaactggaat gtcagctgcc tcaataatgc
    11941 ttttagtaat cgctggatga ggtgtaaaag gatacacagc aattactgca ttcgcatttg
    12001 tattacgtat gatcgcaata tccgtagtaa agatgatcga acggatcaat tttccaaata
    12061 tcttgatccc gcttgcttct aaaatgattt ccggtactct tacgatatct tttctcaatt
    12121 ccgtcatgat ttctggcgta aattgttctg ccatcagcca cttctccttt gctatctccg
    12181 gtcatactga ttcaattgat acgtctcgat gacctggatg attttttgcg cataaccggg
    12241 gtctgtcgca taacctgcat cttgcaatgc ttgtgcggct tcctgatagt tggtggccgt
    12301 aatcactttt tcatacagcg caggattcca atctactcct tggacgaaca gttgtgtatg
    12361 gtcatccatg gactgttccc atgaatcata cactttgaag tctccttgga tagttatcca
    12421 ttcttcgtta acaaattcct ttgtttccag gcttactttc ttctgatccc cataagcctt
    12481 gatcccgaaa agattattgt atttgctagc caaggtactt tttccccaat ttgattctaa
    12541 tatagcttgt cctaaaatga tactaggaag cactccataa ccatcttgta gttcttgggc
    12601 atgcggaaca agccgttcaa taaattgctg atgggattgc gcttcatttt cttgctgatt
    12661 atcagtaaaa ggagaagaca gattcttcaa ggaaaaaaca aaggctaaga gtatcactgc
    12721 tacaccggca accactgctg gccaacggat attcttgcgc ttttgctttt tccacttttt
    12781 tttcgccatg atcaattcct acttttcatt taattcattc agatattctt ctagcgtcaa
    12841 ttggtgtttg gtgatatact cagcgttttc tttaccgaca taacgtaagt gccaaggttc
    12901 atatgtgatc ttagttatat cttcgtgtcc ttttggataa cgaacgataa atccatactg
    12961 cgcagcatta tctgcgatcc atttagctcc aggctgatct ccgtaacttg catccaatac
    13021 agtagaagga taactgttgt accagttttg atcgacgaca tcgactgcca gacctgtgtg
    13081 gtgttcgctg tatcctggct ccgtaatcgt ttctttagta atctttgtcg cctcctcttc
    13141 tgatacacct tgacgactca tcacatcttg gacattttgt tcgaaaactt gttgttggga
    13201 tgatacagac cggtaagctg agaccatcac taatggaaat ccagctgctt cagcagcctt
    13261 ggcaaatgct tcatattctg gagcaatgcg tttgtcgatc aagtatccgt tacttaatga
    13321 tgccaattga gtagactcat caatttcttt ttctaatttg tgatctggac caaccaaaac
    13381 tagcttccaa tcgtctgttt taacatctgg caattgtttg gacgtacgat cttttgtttc
    13441 tttgtccttt accttgctgc tggttgtttg actgttcttt tctttactag cagttgtttt
    13501 ggtgcttgtt cgttcgactg cttgattgcc ggcttgaaca tgcgaatcat catcgctaaa
    13561 aataacatag cccattgctg cgatcataat cacaactgct ccaaatccta aaaatttatt
    13621 cacttgaaaa atcctttcgt tgttcttcac gttccccatt cgcctcatga acgtaagttt
    13681 cttccagatt ttcattcacc caatcgagaa gttcgatttt ttctccttcg atctgttttt
    13741 gaacatctgc tggcagacga aaattcaaaa tatcatcata gccccattca tcatattgaa
    13801 tacttacttt caagtcaagg tatctaccag cctgctgatt atttttcaaa gggatcagct
    13861 cgacttttgc tgctttgctg ctaagccatt tttgcgcgat gcgtgttttg atttgcttgt
    13921 actctgcaac ggcaacgttt cgtttttcgg gaaaaataat cgtttgacgc aaaatctttt
    13981 gcgttagcat ccattcataa tcagtgaaaa gattttttac tttctgcatc gcttcagatg
    14041 gcagattttt ctcgccatct ttccattttt gccattgttc tggactgatc agcaagtatt
    14101 catctaaaaa atttttttca gtttcaaact gttctaataa tacactcata ataagatcaa
    14161 tatatacttg atgcacgttt atcccctcct tttaactatt ctacttaagt tttgatcaaa
    14221 aagcacctct taaatcgctg ctttcatttt ttttaatgtt tcaagtcctc tcctctttat
    14281 tcttcgtgat ttttgttata ataatagaca gacaagaaac tcaatccaac tatgaaggag
    14341 cgttttcaga tgaaaaaata tcaatgggga atcgttggtc ttggtaccat cgcacatgag
    14401 tttgccgaaa gcttcaatca agagacgagc gaactaacag cagttgcttc cagaacttca
    14461 gaaaaagcgg aaaacttcgc tcaccgttac aatattccaa aagcttacgg aagctatcaa
    14521 gaaatgttag acgatgctga aatcgacatc gtgtacattg ctgtaccaaa tagacagcat
    14581 atcgaccata tcttggcagc attggaagca ggcaaacacg ttctatgtga aaaagccatc
    14641 acgatgaata aaaaagaact ggctgacgct atgcgcttag cagaagaaaa gaatttgatc
    14701 ttagcagaag cgatgacaat cttcaacatg ccactttatc agcagttacg ctcaataatg
    14761 gatacaggaa aactgggggc tttaaaaatg atccaagcac ctttcggcag ttacaaagaa
    14821 ccagacccta aaaaccggtt cttcaatcca gaattagctg gaggagctct gttggatatc
    14881 ggtacgtatg ctgtatcttt cgctcgtttc tttttgagct ctcaacctga agtagttgcc
    14941 tctacaatgg ttccttttga aacaggcgtg gacgaacaat ccgtcactat cttgcgtaac
    15001 aaagaaaatg aacttgctgc tgtcagcttg acttttcagg caaaaatgcc aaaagtcggt
    15061 atcgttgcct ttgagaacgg ttatgtgacg attgctgatt acccgcgtgc tgatcgtgcg
    15121 gagatcttat ttacagatgg tacaaaggaa ttcatcgaaa gcgggaatac atctgaagca
    15181 atgaattacg aaataaagaa tatggtacac atgattgaag gtgacttgcc caatcgctct
    15241 ctcttcttga caaaagatgt catcgaaatc ttggatcaaa tgcaagttct atggaaaaat
    15301 atgtaatttt aaaaggttgt gaaaaagctg ttctgcatca agtaataaga acagccaaac
    15361 aaaaatagct cctcctattt ttcgtttggc tggacttatt accgcagagg aaagcttttg
    15421 aacaccgttt aagaaagagg ttgtgagcct accgttcagc tccgagtcac aaggagcaat
    15481 ttcaaaaaac agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttgacag
    15541 gagaacaccg tttaaagaag aggttgtgaa atcagctttt tgacctgaat attagctgaa
    15601 ttcacaacct ctatttttgg attttttgtt gggagaattc tgccttgatt tcaataaaac
    15661 ttttaaaaga ctgcaaaaac tggaacaatt ctgctcgatc ctcgaattca gggcgactag
    15721 tcggcagagg catttgccga tacaatacat aaacttcttc aatacgtgtt aaaatttgtc
    15781 tgccatcatt tgactctgaa aatgtttctg cagtataaat caataaacca tagatatgtt
    15841 ttccataata agctggttgc tggattctct ctagattttc cagcatgtct ttaatcacat
    15901 tggcctgtgc tcttctcata gagaagtaag tttcgtagta tagcggctgt ctgagccagt
    15961 agttttcttg atgttctctg gcatcttctt ggctttcgcg aatgaatgtc agtaaatgtt
    16021 cgcatttgat ctgcacatct tctttatttt ctgataaaag aaattctgcc atttgtctca
    16081 aaatatttcg aaattctttt tcgatctgta tttggttttc cttcagctgc ttcgtacgat
    16141 ctggcatgta aacatttgca agcaaagcaa atactaatcc aatcgccatt aatcccgctt
    16201 cattgccgat cagctgccaa gacatgtttt cctccactaa ataatgagtg accaaaaccg
    16261 aatttactac tatcccttcg gttaatttga agcgagaggc aagcgggatg aacaggaata
    16321 aaaaaagact gaagccaaaa atagtatacc caacagcaaa aaaactgaac agtgctgttg
    16381 ccgtagctaa tataaaggct gcgattcgat tcatgcccgt acgcaatgta gagcgcttgg
    16441 tatttcctac acttaataat gcaatgatcc ctgcagctgg ccaatacaac agcgataagc
    16501 tacccgctgt gaccatcgaa aagaagacac tgatcactgt tttgactgtt cgcattccta
    16561 tacgcattta cttccccctt ttttatttta attatacttg aataatgaaa aactagctat
    16621 cagacaaaag tttaggtatg atagtaaaaa gaccaaaaag gagaatctct atgcaatttg
    16681 gccgttttcg actaggtatg cgtacgatca aaagtgcctt agctgttttt ttatgtatct
    16741 tgttttttca tttgactcaa cgaggaatcc cgatgattgc tgcattatct gctgtttttt
    16801 ctttgcgtca ggacttgact acaaccgttt cttttggaaa gtctaggatc atcggcaata
    16861 cattaggtgg tacattagct atcatttatt tttttgtcaa aagctatttt cacaatgatt
    16921 ttttggtaga attgatcttg cttcctattc tagttattat tgtgatcgtc gtgtcagatg
    16981 gtattaacaa taattccggt atcatttccg caatagctac gttgttgctg attgcgttga
    17041 gcatccctca aggcgaatcc gctttgtatg ctatccaacg tgtactagat acttttatag
    17101 ggacattcat tgcaatcggt atcaatttct ttcttcgtcc tcctgaaaca gaaaagaagg
    17161 aagaactatc agaagatttg gtcgaactcc aaaagaaaga acgattgcta agaacaaatt
    17221 tacaagagat agaagctaag atccaaaaac aaaagaaaaa tagcgaggac taattttctt
    17281 ttgcaataaa ataaaaaaat ggagagaaga actttaaaaa gtcttcctcc atttttgatt
    17341 gaacggtgaa gtgtactact ttattcttcc ttcatcgagt tttgttctgc ctcttttggc
    17401 gcttcataca tatttacttg gctagtagtc ccacgtgtcg acaaattctc tacgatttcc
    17461 ttcaagtcaa gtcctgttgt cgcttttagt gtttcttgag tagaagaaag caaatttgtg
    17521 gcataatttg tcactcggtt cgctccactg gtttctcctg aactgcttcc tgtatcgaca
    17581 acagagattt tttcgatatt tcctagcggc tgtgctgctt cgcgcatcaa ttgcggtagc
    17641 atatcgataa ccatactcaa gacagctgct tcgccatatt ccttgaaggc attggcgatc
    17701 ttttgttttg cttcggcttc tgcttttcct tttgctagtg ctgcttcggc ttctgcttgt
    17761 ccagctaaac gtgtcttatt ggcttccgct tctgccagtg cttccacacg gaatcgttct
    17821 gcttcggctt cagtcacttc tttgactttt tgagcttgtg cttcttgttc tttagcgtac
    17881 cgatcggcat ctgctttttt cttcacttcc gaatcatatt gcttttcacg acgtgtgatt
    17941 tctttttctt ccagttcgat ttgtttttga cgttctacga ttttaatctg catttcctgt
    18001 tcgattactt gttgttgtgc tcgggcactt tctaaattgt aggcttggtc tgcttttgct
    18061 ttagcaacat cttgttcttg tttgtaggtt gctaatttca gttctttttc ttttaatgat
    18121 tctgcgatct ctgtttgtcg ctgtaattca gctgcttgtg attctttttc tgcttgagct
    18181 tttttgatac gcgtttcttt tagagcttcc gcttctgcta tatctgcatc tcttttgact
    18241 tgggcgattc ttggtttccc taaagagtca agataaccgt ttttgtctct gacctctttg
    18301 atagtgaaag aaacgatgat caaccccatt tttgcaaggt ctacagaagc gacttcttgt
    18361 acgctttgac tgaacttatc gcgattttga tagatttctt ctacagtcat agaaccaaga
    18421 atcgagcgca agtgtccttc taatacttct cttgcttcat tttccagttc ttctcttgtc
    18481 ttgcctaaaa actgttctgc tgctgtagca atctcttcta cagatgaacc gattttgatg
    18541 atcgaagtac catcacacat caccggtacg ccttgttccg tatacacttc aggggtggag
    18601 acgtccaatt tactcgataa tagactgatc cgattcgaac gttgaaaaac tggcaagacg
    18661 aatgcgccac ctccacgcac aattttgatt ttgttattgc tttcatcaat gtgcacattt
    18721 ttgcttccta aataacttcc actgatgatc aacgcttcat ccggttttgc tgtctggtac
    18781 ttcgctacaa agacgatcag caacatcagt aaaatcaaag ctgcgattgc gatcgcgata
    18841 aaaacaactg gtatttccat ttaacacacg ctcctttaag cttttatcat gctttcataa
    18901 ggcatgacat aacagattct ttccttgatc tcaatgatca atactttcgt tccccgctca
    18961 gctgtctgtt cttgggggct gtaaaaacaa gctggacgac taatggaacc ggtaacactt
    19021 ttcaactgaa tttctcccat acctcgtaca gggatcggtg tgatcacaac agctacttgt
    19081 ccttccagag ttttttcaga agacgacatc gttgattcgg catgtttcat cggcatcaat
    19141 atatagaaat taagcaaaaa taccaacact atcgatatgc ctccactagc aataaataaa
    19201 aataaagatg attgatctgt taaacgctca cctagaaaac caaataagct tgtgaaagtt
    19261 aaccaaggga taataagcat cggatcaagc gggccatcaa aatcgaatac atctccaaaa
    19321 ataatcaata ggactgccaa tcctgcacaa atcagcaatg tataccaata aatcgtctca
    19381 agtgcgattc cccctatcat atacacgcct ccttccattt ctacgtctat cataccaatg
    19441 tttgaaaatt ctgtatagct tcttttaccg actcagtggt gttttttata ggaaggttgt
    19501 gtttatcgtt gacaatttgt taaatttttg ttttcatgtt aaaattttca caataaattc
    19561 tttcgaggta gcctatgttt ccttttttta agaaaaaacc attaaagctc cagaacacga
    19621 tcatccttgt cgtttgtttc tccacattcg tttctttgat tgtttctgcc atattgatca
    19681 ggaattttat tctttcgatg aaccgatcta gtgtgtcaga taaaatctct agcattgcta
    19741 aaatcgttgc caatgataaa ctcatccaaa aaaagctgca gactggtgac ccgacacatg
    19801 cgattcaagg aataacaaat gaaatcatga ttgcatcgaa tttagatttt atcgttgtca
    19861 tggaccctga aggcattcga tattctcacg cagacccaag ttacatcggt cttcctttct
    19921 caagtcatga agatgtgaaa aaagtgattg gaggacaaga atttttctct gaaaaagaag
    19981 gaatgttagg gttcggcttc cgctatttcg tccctattta ttatgatcaa gagattattg
    20041 gtgcagtttg tgttggctta acaaacaaca cgattgacaa tcaagtcaaa gatggacaaa
    20101 aaatgattgt tttttcattg attattggac ttagcgtagg catcataggc gccgtttttc
    20161 ttgcccaaaa aatgaaaaaa gtactgcttg gacttgaacc acaagaaata gcagaacagt
    20221 taagtgaaaa aacgattatc gaaaatgaag tagatgaagg aatcttagcc atctcttctg
    20281 ataaaactat cctcttgatc aatacacctg caaaaaacat tttctaccaa acagagcagc
    20341 tattggattt cgaagtagga aaaaaatcag tgataatcta tatgacgttt tgtttaaaga
    20401 gacgattgag catcaaacga ccgttcggac tcaatccatg tatttaaatg ggatagaaac
    20461 tatttctagc gttacacctc tttttagcaa tgaccgtttt ttaggagcag tcgcaacttt
    20521 acgaaatcga tcagaagtaa tgaaattaat ggaggagctt ggggggacga aacaatatat
    20581 aaactccatg agagctcaaa cccatgaatt catgaataaa cttcatgtca tttctggttt
    20641 gattgatttg aagaagtatg aagaagtcac ccattttatc cagcagctaa atcaaaatta
    20701 taaaaaagag attggccaag tctcaagtct gatcaaagta cctgcaattg ccggtttttt
    20761 attaggcaaa ataaacgaag ccaaagaaca atctgttgaa atgctgttag aaagcgattc
    20821 ttttatccct aaaatggaga tggatgactc catccattca cttttgcaag tgttagggaa
    20881 cttacttgat aatgcaaaag aagccgtgct acaaaaacat gaaagaaacg gacaggtccg
    20941 gattcattta aactatgaca cagaagctga agtattcatc gtgaccgtct gcgatgacgg
    21001 tgtcgggata tcagaagaaa tctcagaaaa aatatttacg ctaggttttt cgacgaaggg
    21061 aatcaatcgt gggtttggat tgaatctcat ccattcaatc gtcaaacgcc acggtggatt
    21121 tttagaattt tccaaacaaa caaatggcgg tacaatcgtc tatctagaat taccaaaaaa
    21181 ggaagatcaa aatgatgaca aatgtcctga taatcgaaga tgatccaatg gttgcagaat
    21241 tgaatcgtac ttttgtggaa agcatcccta atatgaacgt aatcgaaagt ttcagaaatg
    21301 tagaagaagg aaaaaaatat ttcagacaaa aaaacgcaga tctgctgctg cttgatgtat
    21361 acatgcctgg cgaaagtggt atccattttt tatcttggct tagacagcaa ggaaatgata
    21421 tcccagtgat tttgatcact gctgcagatg atatcgaaac cataaagaat gtgatccgct
    21481 acggtgcctt agattatttg atcaaaccgt tcactttgca acgttttcag ctggcgatcg
    21541 aaaagtatct tgctttctct cagacaatcc aaacgactga aaaagccgat cagaaattga
    21601 tcgatcaagt attcaaccac accactccca ttcacgaaat accatcattc atcgatttgc
    21661 ctaaaggtct gtcaaaactg actttaaaaa aaataagtgc atttattcta aaagaagata
    21721 ctattttttc aacaaaagaa ctggctcaaa aaagctcact gtcacgtata tccgtcaaga
    21781 aatatttgaa ttttttagta gaacaagata tattgaacga aagaatcaaa tatttggaaa
    21841 tcggccggcc gctaacctgt tatgaattga caaagaatgg gcggaaaaag ctcacagatt
    21901 actgttgagt catcgaatta tcccatacaa aatagtccaa gtatacagaa gtaacaactg
    21961 tttacttgga ctatttttaa acattttatt gacgaatatc catgttttct tatcttaatt
    22021 gacgggcttt aggaagagat acctcatttt tcgtttttcc agtttctatt atttcacgaa
    22081 agttctcgaa acttgtcgaa atcatatttg ataatgcctc atctgtattt gaacctacat
    22141 gcggggttac aagaactcta gggtacattt ctactaattg ctggatagca gggtctggaa
    22201 tcgtctccca tggcttgaag gatcggaaaa aaacctctgt ttcatttgca aaaacatcgg
    22261 ttgcaaaacc agctaaatga ttgcttttca aagctcttaa aatagcttca ttatcttgaa
    22321 gctctccacg ggctgtatta atcaagatgc tacctttttt catttgagct aaaaatcctt
    22381 cattgatcat ttgatcgttt tcacctggga tatgaggtac atgaatactg acaatatcgc
    22441 ttttggctaa taactcttca agcgagtgga acgtcaatac ttttttagca gcttctgtct
    22501 gatgaatatc gtagccgatc actttggctc ctagtccctt aaacaaaaca gcttccgtca
    22561 accctatcct gcctgttcct atgatcccta ctgtgcaatt tctgatttca cgactaaaca
    22621 tttgctcatc cactcgaaaa tctttgaaag atgttcggat cgtcgtatac gcagtatttc
    22681 tcaaaagcat catggcaaaa gtcaaagata actctgctat cgcatttggc gaatacgatg
    22741 gtacacgggc tactgtcata ccaaaatcag ctgccgcttg tagatcgata tgattgactc
    22801 ccacagtccg tgtaaaaaca taacgaatcc catattcctg catttttgca agattggtct
    22861 catctgctat acaatttccc ctcaacaata cagcatcatg gtcctttgct gacacgatat
    22921 tgtcatgtgt caacagttct tcataaagac tgagatcata atggtaaata ttcaattttt
    22981 caaaaaaatc tatctcgttc ggacgaacac cgtaacatgc gattctcata ctttcttcct
    23041 ccatttctta tgaaaataaa cctatgccat gaatcatttg taatacaacg atccatacag
    23101 gtgtcagtac cacagcaaaa atagttgaca gtaaggatgc attggaggct aaaagtgcct
    23161 ccctatcaaa actgatagca taggctgctg ccactgttgc agctggtgta gccatcatga
    23221 taacgattgt tgccagtgct tcaaaattga ctggtagaat attcgttgcc gtcaaaactg
    23281 ctagaatcac gatattcaat gcaggtacaa ataccacttt gactagactg taataccatg
    23341 aagttttttc agatgcagct tttttgaagc ttacttcacc taatgttgcg ccgattgcta
    23401 gccacgccaa tggtgaactt aaaccagcta aataatccat cggctgaaat agccacggtg
    23461 cagtctggtc aattcgcaaa aacgctactt ggcttatctc tccttctgca cctgtaactg
    23521 acacttgtgg cagataccct tggaacagcc aaatgaaaag gccggcaaaa gtagcgataa
    23581 tgattggatt gacgaacatc gctttgatat ttttagcttc catcttcaag ccactcattt
    23641 ttatgtatcc ataagaatac aaaaagattc ggtagccaat attgaaaatt gaggaataca
    23701 tcacgccgac aggaccataa atcgcactga cgataggcgt tccgaaaaag gtcgtagaac
    23761 caaagatcgt caggacacgc aacgttgttt gcttatcccc tttatagctt aagaaaaaag
    23821 gttttgaaac tagaataaga ataatataaa taactaatcc ccaaatcaag acattgattc
    23881 cttgcatcag taattctgta ttgatatctt gcatgaaagc tcgaaatgcc agtgctggca
    23941 aagccactgt taagacgact ttcgatagaa tttttccaac agattcagaa aaaatcccct
    24001 tttttctaca aacaaaccct aataatataa taaaaactgt tgaagtgatt gcactgacaa
    24061 tcgacatatc tgtcaaagtc gatcgaaaaa tgtcaaataa gttcatcttt ctcctcctaa
    24121 ttatgttcat ttattcacaa taaaaaaagc ttttattttg ctaatacgta ttaacatttt
    24181 taattttatc tttacattgt tacttattga acaataggaa attaaagaaa cttttgaaac
    24241 ttatgtaaa
//
LOCUS       DAZUJO010000036        24064 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000036 DAZUJO010000000
VERSION     DAZUJO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.036"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(103..252)
                     /locus_tag="ACG8OL_001545"
     CDS             complement(103..252)
                     /locus_tag="ACG8OL_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817538.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            1220..1546
                     /locus_tag="ACG8OL_001546"
     CDS             1220..1546
                     /locus_tag="ACG8OL_001546"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4817539.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1554..2462
                     /locus_tag="ACG8OL_001547"
     CDS             1554..2462
                     /locus_tag="ACG8OL_001547"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4817540.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            3088..3342
                     /locus_tag="ACG8OL_001548"
     CDS             3088..3342
                     /locus_tag="ACG8OL_001548"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFS4817541.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3884..4042
                     /locus_tag="ACG8OL_001549"
     CDS             3884..4042
                     /locus_tag="ACG8OL_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817542.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            4114..5340
                     /locus_tag="ACG8OL_001550"
     CDS             4114..5340
                     /locus_tag="ACG8OL_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4817543.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(5429..5821)
                     /gene="rpsI"
                     /locus_tag="ACG8OL_001551"
     CDS             complement(5429..5821)
                     /gene="rpsI"
                     /locus_tag="ACG8OL_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS4817544.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5837..6265)
                     /gene="rplM"
                     /locus_tag="ACG8OL_001552"
     CDS             complement(5837..6265)
                     /gene="rplM"
                     /locus_tag="ACG8OL_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS4817545.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLAGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6799..7266)
                     /locus_tag="ACG8OL_001553"
     CDS             complement(6799..7266)
                     /locus_tag="ACG8OL_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4817546.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7386..8924)
                     /locus_tag="ACG8OL_001554"
     CDS             complement(7386..8924)
                     /locus_tag="ACG8OL_001554"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS4817547.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9004..9690
                     /locus_tag="ACG8OL_001555"
     CDS             9004..9690
                     /locus_tag="ACG8OL_001555"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS4817548.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9891..10418)
                     /gene="msrA"
                     /locus_tag="ACG8OL_001556"
     CDS             complement(9891..10418)
                     /gene="msrA"
                     /locus_tag="ACG8OL_001556"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4817549.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10502..11179)
                     /gene="pgmB"
                     /locus_tag="ACG8OL_001557"
     CDS             complement(10502..11179)
                     /gene="pgmB"
                     /locus_tag="ACG8OL_001557"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4817550.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11176..12304)
                     /locus_tag="ACG8OL_001558"
                     /pseudo
     CDS             complement(11176..12304)
                     /locus_tag="ACG8OL_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12304..14487)
                     /locus_tag="ACG8OL_001559"
     CDS             complement(12304..14487)
                     /locus_tag="ACG8OL_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS4817551.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14484..15509)
                     /locus_tag="ACG8OL_001560"
     CDS             complement(14484..15509)
                     /locus_tag="ACG8OL_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4817552.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15554..16381)
                     /locus_tag="ACG8OL_001561"
     CDS             complement(15554..16381)
                     /locus_tag="ACG8OL_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4817553.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16378..17445)
                     /locus_tag="ACG8OL_001562"
     CDS             complement(16378..17445)
                     /locus_tag="ACG8OL_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS4817554.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17471..18307)
                     /locus_tag="ACG8OL_001563"
     CDS             complement(17471..18307)
                     /locus_tag="ACG8OL_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817555.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18304..19173)
                     /locus_tag="ACG8OL_001564"
     CDS             complement(18304..19173)
                     /locus_tag="ACG8OL_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817556.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19243..20526)
                     /locus_tag="ACG8OL_001565"
     CDS             complement(19243..20526)
                     /locus_tag="ACG8OL_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4817557.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20540..22225)
                     /locus_tag="ACG8OL_001566"
     CDS             complement(20540..22225)
                     /locus_tag="ACG8OL_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS4817558.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22500..23525
                     /locus_tag="ACG8OL_001567"
     CDS             22500..23525
                     /locus_tag="ACG8OL_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4817559.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7002 a         5003 c         4312 g         7747 t
ORIGIN      
        1 tagtcattct ttacaacgat ttatagaaaa acaacctctt taaatataaa aggtacgggt
       61 tactgattac ttgtaaatct ttacgttcca gttttattct ttctaaaaaa tatactcact
      121 tcttttctta gacttctttt tttgattctc ctctttatgg tcttgtcctt ttttgccttc
      181 aaaatattta aataaacccc tttcaaaagc atttctaggt tttggacaat aaggaatgac
      241 ttttttcttc atataaatac ctcatttaac tatttgatat catcacatta tcaaaaaaaa
      301 taggtttttt attaaaacta tcttgacctt tcgggagatt tcactgtgat ttatttttat
      361 tcccatactt ttttcgttgg tcactttcgt atacctaatc taagcgatca tcggcgacgg
      421 tgcttttaca acgaatggca ggacaaaaca aggggcggaa aaactttttt gcctatttca
      481 taaaaaactt tttcctacag caatcccttg ttttgtccta ccacacgttg tgaacccaaa
      541 gcacgccgac gaacgcttag ctatacgaaa atgacctcca aaaacaaaaa agtgaggtga
      601 atagaaacaa aaagaaattg aaatctccca aaatgtcgtc cgcctcggag acaacttgct
      661 gctgaaaatg gttggttaat tcatttaagt attaaataag ataaatcatc aaattaatcg
      721 gaaaaatagc atgattggcc ttatcaaaaa taaactgtaa aaacttatat ttctatgagt
      781 ggtggaacaa tgctaatacc aaattaattt atcgattacc cacctgcaca aaccaaagat
      841 agcattatta ggaaacgaat caatcagcga atccagcatt ataatgagga tactaaaaat
      901 aaagttttca taattataaa tccgccttcc tacgaaaggc accaatgagg tgatgaaaac
      961 cttagagcgc tttctttctt tttttattcc gtgttaccct ttccttgcag gtccctggta
     1021 cactacagaa cccgaggagg tgagtggcgg ggctgtatag cggcaacccc gcataattat
     1081 atggtgtcgg tcatccgtta aggcatcaat gaatggcgca aacccgtagc ccgtaaactt
     1141 atctcttccg aagttgactc gatttcgccg gatgaccagt ccctaccaaa cctgtactat
     1201 ttttttacag gaggttgcta tgttcaaaat ctttcgtaaa aattgttgtg gcttggatgt
     1261 ccacaaaacg tggatctatg cgtgtatcgg aatcacagat gcgaatggtc gtacagtgta
     1321 taagcaagct cgcttctctt cctttacaaa aggtctcaat gaattagctg aatggcttgc
     1381 taaatacaac tgtaaggatg tgtgtttgga atcttccggt aaattttgga ttcctgtttt
     1441 taatattctt gaaaaaactt gttttgtcac actggctcat ccgaagtaca ccaaacccca
     1501 gaaaggaaat aaaacagatc gcaaagatgc aaaaatggat ttgtgatctc tttatgtgcg
     1561 acatgatcaa acccagtttt attcctggtc ttgaaatccg ccagcttcgt gatctaatgc
     1621 gttatcggac gaaactgacc aacatgaaaa caagtgaaaa gaatcgtgct ttaaactgct
     1681 taaccgtttc taatctgaaa ctagatgatg tttttagtga tgtgttcgga aaatcatcca
     1741 cttctatcat ccaatacttg ttagaacatc ccggtgaatc atttgatgtt cgaccattta
     1801 tccatgcccg ttgtaaaact cccgttgaag aaatccaagc ggcagttgag ggggcgattt
     1861 ctgtacctca agcgattaag cttcgccaat gcctcaacca tattaacgag attgagaagc
     1921 atcgagaaga gatcaatcta gagattcaag ctcttgcaga acccttctcc cctgttttgg
     1981 aactacttcg taccataccg ggcttagaca ctcagccaat aacagcgatt gcgattcttt
     2041 cagaaatagg accggatatg tccgtgtttc caacttcgaa acactttatt tcttgggctg
     2101 gatgctgtcc aagaaacgat cgcagtgccc gacgaacaaa atcaacacgt atctctcgtg
     2161 cagggcagtc gttgaaaccg ctacttgtac agattgccaa tgctcttctc agatccaaag
     2221 aacatccaga atttaaaaat cgctatcgta gaatcaaagc gaatcgtgga cacaagaaag
     2281 ctatcattgc tatttgtaag atgctcctga ccgctatctg gaatgtctta tcaaaggtgg
     2341 aaccctattc tgctaaagga tatctggagc caaaacctgc gaaagaagag aaagttctga
     2401 cgcaatccca agctctggac cttttaagaa aacgcggata taccattata gatagtgctt
     2461 aaaccaaagt tacgttgatt cttcctaaat agactcgttg tcttccattt caaaaatttt
     2521 gaaaacggta ggcttattta gtgtaccgtt cttcaggtat cttcaaatag atttattttc
     2581 aaacttattt cctttcttct tttactctcc tatagcttag catttaagtg ggcactcgtg
     2641 tcaccttgct agatagctat gaatgccgta ataagcctcc tagtatatag gcagactgcc
     2701 aattataact gattcaatcc acattatgat aaaatccacc ttttcgtttc atatgtgttg
     2761 aaatatcaca tatgtcaaaa gtgcacgagt cttttcattc agtgctttgt aaatactcaa
     2821 aattattcct tttcacttta aaaaaaagat aattagtaca aaatcagaaa tgaggaaagt
     2881 attaggatgg actagaaagg agttgttgaa tttgttgcaa cagattggtc atttatcaat
     2941 taaaaattag gtgaagtttt attcatcact gcagctgctg tttcccagag aataaaacgt
     3001 ctagaagaaa ctggtcagtt actgtattat caagcagcaa ttgactacga acaagcttga
     3061 ttactaacca aagcgtttat ccacctgttg attaaatcaa atcaaaaatc aggtttttat
     3121 acgttcataa aagcaatctc cagtgtactc gaatgtgact gtatcatagg tccttattcg
     3181 atgttaatca aaatggggtt tccttctacc gaatcattgg acagttttat caatgattta
     3241 tcaaagtttg gacgaaccaa cacccaaatc gttttctcga ctcttgtttc cttaatggtt
     3301 tatcagttct ggaatgttta tcatgcgctt ttattaaaat agaaaagagc ttttttcatg
     3361 cgacaaagca gtgatcattt attggcacat gcaatctttg tggatccatt ttttgaaaat
     3421 acgatattta gttttgaccc aaaaatcttt aaatacgtat ttgataattt atcttaacaa
     3481 ccagataatt ttattcagca ccgtattatc tatattgaat tctcggaatc aagataaatt
     3541 tttttccgtt tttgtttctt ttttaatctt cttcttgtta ttaatagtga ttaggatgat
     3601 attcctatat tgatctttga taattagata taatttttga ataacgttcc ctgctattcg
     3661 agctagaaat acaagtacgc catgacctag ttagcgacaa ctacttagag atttcttttg
     3721 ttctgggaca acaaaatgcc atgacaatag cagtaaataa tgatacttcc gtaaggctcg
     3781 ccgtaaactg gggagaggtg aaattcctat gtgcttaccc aaagcggttc aaaaatttta
     3841 aactattatt ttaggagatg attttataaa atcgaaagcc tacttgaata aaaaacaagc
     3901 ctgtgaattt ctaggaattg ataataatac tttagataag tggattcgtg tatataattt
     3961 tccagtaata aaaattgatg gcgtagttcg ctttgacgta gaggatatca aagatttcat
     4021 gaatacattc aaaaaaaatt gaaattttct tctcgtcaca tatactataa atagcgaaat
     4081 gtactgcctt actttttaaa ggagtgagga agtttgccta caaagctttc aaatgggaaa
     4141 tataaaacta atctgaggta tccaaaaaaa ttcagagaaa tcactggaat tgcatcagag
     4201 aagtatcaaa aagtatttcc aactaggcaa ttagctatta aggccgaaag ggaaatgaaa
     4261 aagaagattg aaacagtttt aagagaggag aatgcaaact cactagaatt gaaaggaaaa
     4321 ataaaattta aagattttta tgaatcaaaa tggcttccta gatatgaact aggtcaaact
     4381 actaggtcaa acagggctcc aagtaatgta acaattagta atactaagga tatattccga
     4441 ttgcacatcc ttccaatgtt tggtgaatat gcaatgaatt atctgaattc aaatactgag
     4501 ataatatcag atgaattaac aaagaaatcg aaagaatacg caaatataaa aattatcaaa
     4561 ggctatgttc gctctatgtt tgatattgcc gaaatactat gttatatcga attcaaccga
     4621 actaccaaaa ttattcaatg tattaccgcg cctaagaaaa tggctttaga agaaaaaagg
     4681 ataagggaag ggaatcaagc attatcatca gaagagctga ctgattggat agaagcagta
     4741 aacaatgact taaataatca cttgcttaca cttcacgatt acacactttt catgcttacc
     4801 ttatatctag gtgatagaaa aagtgagact tatgctctgc aatggaaata tattgatttt
     4861 aataaaaata cggttcgtct caaatatgct ttagataaat atcaaagaaa gacacataca
     4921 aaaggttgga aagacaccgt tattcaggca ccagaagttg tgatgacatt attaagagaa
     4981 tggaaatcag ctcaggctga acaattgttg aaactaaaaa ttatacaaac acctgaccag
     5041 tacctcttta cctattccaa accttctgga gaagtaaatt gtcctgttca cgctgactac
     5101 ttaaactatc gaatcaatgc aataaaaaaa aggcatcctg aattggctca attatctcca
     5161 cacaaacttc gtcataccta tgcaactata gctagggaag gtggagcaaa tatgaatcaa
     5221 atctctaatg ctttaacgca ctcggatatt tctactacaa aaatatacgt gaacacacct
     5281 gacgtagttg ataaatctgt ttttgaagca tttcaaaaag gattgaataa gtgtgattaa
     5341 catgtgttct caaatgtgtt cttattataa aaaaagacac tttccaaata attgaaaagt
     5401 gtcctgaaat attgatataa aaagaatatt aacgttttga gaattgtgaa gctttacgag
     5461 cttttttaag acctggtttt ttacgttcga ccatacgtgc gtcacgagta agtaagccag
     5521 cacgtttaag agctaaacgg aagtcaggat caacttctag caatgcacga gcgataccat
     5581 gacggatagc tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat
     5641 atgcgccttt tgtttcagta acagcgaaag gttgattgat tacttcacgc aagtcagcat
     5701 gtggaatgta ttcttcaaca tcttttttat ttacagtaat tttgccagtt cctggtacta
     5761 gacgtacgcg agcaactgcg tttttacgac ggcctgtgcc gatatattga acttgtgcca
     5821 atgaaattcc ctcctattag attaagtttg tgatgtctaa aacttctggt tgttgtgctg
     5881 catgtggatg ttctgctcca ccgtatacgt tcaattttgt aaattgtttg cggcctaaag
     5941 tgtttttagg aagcatacct tttacagaag tttcgatcaa acgacgagaa tttttagcac
     6001 gcaattcgcc agcagtgatt gatttcaatc ctcctgggta gttgctgtga cggtaataaa
     6061 ttttgtcagt cgctttttta cctgctaatt tcactttgtc tgcattgatg acgatgacga
     6121 aatcgccagt atccaaatga ggtgtgaaag ttggtttatt tttaccacgt agtacagatg
     6181 caacaactgt tgaaagacgt cccaatggaa catcagttgc gtctactaca taccatttac
     6241 gttctacttc gcctggtttg gccatatatg ttgtacgcac gttattttcc tccaatttaa
     6301 gttctgatgt ttgacataca cttcgagttt ccggggctct ttgtggggca aacaatacca
     6361 tttaacatat tacccgctaa tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt
     6421 tatatgaaac tttacttctt ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg
     6481 aaaaacaaaa agaggctgtg acaaaagtct tgtcacaacc ccttattttg aataaacggt
     6541 gttctcctgc caactcctcg gtcacaagtc acaatatcca taaaacataa gaagctgttt
     6601 tttaaaattg tttcttgtga ctcggagctg aacggcaggc ttacaacctc ttggtaaacg
     6661 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     6721 ttgacgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctttttcggt
     6781 caagttcaac aacagttttt acgcgtcgat ctctggcgct ttgcccaaat gagcttggat
     6841 catccagata cgtttttctg tggctgtttt gaattcgata aagatatctt gtgttggatc
     6901 gtcgccttct tctccagaaa cttcgattcc tttttggtaa agatctgcta gataacgata
     6961 gcctgcaaca agtgtttcca aacgttctgg aatagtacga tcccagcggc caatttcatc
     7021 agggattttt gtgttatctg caaattcacg caatgtagaa taaggtgaac cgtctaatgt
     7081 gatcaagcgt tcggatactt catctaactg agcgttcaaa tcatccatga actcatccat
     7141 cattgggtgc aatgtaagga aacctggtcc tctcatgtac caatgtgttt ggtgaacaac
     7201 aacagacatt tggcttagat ctgcaactaa ttgatttaat acttctttgg tttgttggaa
     7261 tttcatcttt cttgcctcct aacattttct tacacttaaa ataatagtag actttcaatg
     7321 aaaagtacaa caaaatcatt tacctgagaa cgattttcag ttcgcttttt tatccgtaaa
     7381 gccaatcatt tttcagaatc agctaattct tctcgcagtt gagcaaattg ctgataagtc
     7441 tgtgctaaat gcttttgtgt cgaaacaaat aatggataga ttttctgata ttttttatga
     7501 tcagtttctt gtactccaac ttgctctttc aattctatca attcctgtgc ttcactgata
     7561 ctttggatct ttccgatgct tctccagcct aaaatcacag ctcctaatgc ggatgcttct
     7621 gtgacagtag ggaaaacaag cgggtaaccc agtacatcag ccatcatctg acgaaaaatc
     7681 ggagattgag caaacccgcc tgtagcacgg atctctgtga gctgattttc ctggcaaact
     7741 ccttttaaaa tccgataaag gttgagacag atcccttcta gaatcgcctt catcatttct
     7801 ttttctgtat gttgccgctg taaaccgata aaactgccaa acgcatctgc gtcccaaagc
     7861 ggtgctcgct caccaagtaa atagggatga aaaatcagac cattggcacc cgctggcaca
     7921 aaagcgatag ccgaaagcag tgcatcatat ggattttctc ctttttcttt agcagattga
     7981 acgacttcct gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc attgctagtc
     8041 gctcctccca ctacccaatg tttctgatcc agaacataac aaaacgtctc catttctgga
     8101 tggaactgag gctgagtagt aataaaacga agtgctccgc tcgttcctac ggtcaaagcg
     8161 gcgatcgtat caccaaaagc acctaaccct agattagaaa gcggcccgtc tgcccctccc
     8221 aaaacaaatg atatatcatt tggcaaacct agttctgctg ctgtttttga tggtagacct
     8281 gttacttgat aggtggaagg aaagacttgc ggcaattgct ctattcgaat gttcaagtag
     8341 tcaagcactt cttctgccca ttggaattca tggatatcaa aatatccagt tcctgaagct
     8401 gttccgtagt cgctgatcag ttcgccaaac aatctataaa agatatagtc tttgataccg
     8461 acaaaataag cagttttttt gaataattca ggttggctgt tttgcagcca cttgattttg
     8521 ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc gataaaattc tttgccaagc
     8581 acggtatatt tcagtttttc tgcttcgtcc gctgctcggt tatctgccca agtgatcatt
     8641 cgagataacg gtgttttctc ttcatccaac aatatcaggc catgcatcgc agaggagaag
     8701 gagacaaatt gaatgattgt gtctgaaggc aactgtttaa ttacttcttt tataacggtg
     8761 cagacacctt gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt ttcttggatc
     8821 aaagggtagc gaaaataagc ggatgctgct tcttgggctt catctgtgaa agcaactgct
     8881 ttggtttgtg tggttccgat atctaatccg attgaaacgt tcattttttc acctcaaatt
     8941 ttatgtatgt acaaaaattt agtttaagct tgcgtatctt ggttttgaaa agggggagat
     9001 ggcgtgttac tttattttat cattggttgc tgtatcggat catttttatg tttaaccgcc
     9061 cagcggattc cgttaggtca ctctattatt tatcctagat ctcattgtgt caaatgctgt
     9121 cgttcgcttt cttggtatga attgattccg attctctcga tcatcgtcca acgatttcgc
     9181 tgccgctatt gcaggtgtcg gttgcccttc tactatttgt tggcagaagc cttatgtggt
     9241 ggtctttttg cctggttctt tactttttct tccgcaagaa atttttcgac gttcatatgg
     9301 atgctttcgg ctttgctatt ttcgttgatg gatttatttt acttcatggt ccattctcac
     9361 acgctattct gttcttgggc aattttatgg attttttggt tgcaggccgg cgtcttccaa
     9421 tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc
     9481 tatctcggag ctggcgatac tctgttgatg ctttcttgga gtggtggctt gtctctagaa
     9541 gagttgcttc agatcttgtt tcttgctagt ctgtttggaa tatccgtttt ttctgtttac
     9601 tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt gagcagtgcg
     9661 ttgctgattg tccttcatct taggtattga aaaaaggttg tgacaaacga cttgtcacaa
     9721 cctctgatat atagtgttct cttaccaaat ccttgtgact tggagctagg cgacaggttc
     9781 acaacctctc cactaaatgg tgttcaatca gctgaccctc agtattagct caaaatgctg
     9841 gttttacaac ctctgatata cggtgttcca taagtagctt tttcggcttc ttatttcttg
     9901 acgccccaat gtcgacgaag gaaacctgca cgtccagaac cttctcgata aagattgtaa
     9961 tggagcttgt ttttcttgta ataatcctga tggtaatctt ctgcaggata aaattcgctt
    10021 cttggttcga tttttgtcac gattggctta tcgaatcgcc cgctgtcttg caatgcttgt
    10081 tttgaacgct ctgcaatatt tcgttcttct tcatcattat agtagatcac tggacgataa
    10141 gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag
    10201 atttccacca actgatcata agaaataact tctggatcat attcgatctc tactgcttct
    10261 gtatgccctg ttgtgcctgt tgttacttgt tcataagttg gatttggtac atgcccgcca
    10321 gtataaccgg atgtcacaga aatgatccct ggctgctctt caaatggttt gaccatacac
    10381 caaaagcatc cgcctgcgaa aactgctttt tttgtcatat aaaatccccc ttttttctca
    10441 tcataccttg atttccaaaa ggaggataac gttttgcttg tgacagaaaa aacggaaact
    10501 attatatacg tgatacggct tctgataaat gccaaaacga acgtacttga tcaaaaacgg
    10561 ttaaatcagg aatcacatat tcagcagaag gaagatttat gttttcacct attcccaata
    10621 catgcatgtt accagccaaa gctgcttgac aaccagcttc tgaatcttca atgaccaagc
    10681 atgcatttgg cggtacattc aattgctgtg ctcctttgag aaaaacttct ggatcaggtt
    10741 ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt
    10801 tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg atgtttgttt
    10861 ttttcaagta ctcaagaaca gctattgcac caggcaaaac ggatgtttca tccatttttt
    10921 gaatcgcttg aagatataga ttgttttttt gctcagcaaa ggctgatttc tctttttctg
    10981 agaatttctg ttccattttt ccaaatgaca gcaatcgctc tagtgactcg gttctggata
    11041 ttcctttcag ttgctcgttt tctttctcag tgaatggaat ggacagctgg ttagctaaaa
    11101 ccagccacgc ttcgtaatga taacgagctg tgtctacaag aacgccatct aaatcgaata
    11161 gtgctgcttt gattttcatc gattacctcc ttgaaaatga acgacttgaa gtaaagatgc
    11221 gatgttttta gctgtgcgat gaagattatc agtggcttgc tgtaaagctt cttgtaaagg
    11281 cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg ctttttcgct
    11341 tcctttacca acacttccgc aaatagctat cacaggaacg ccctctggta ctcttctagc
    11401 aatcccaact ggcgcttttc cttttagact ttgtccatct agacgtcctt cgcctacgat
    11461 tacgaggtct gcttgctgta cccgtatcga actccagtaa atccaatacg tagtcgatgc
    11521 cagagactat tttccctgaa gcaaatgcta acagtcctgc acccattcca ccaccagcac
    11581 tactgcccga tgcctcgatt acttgagggg caaatctcat ataaaaatca tgcatggctt
    11641 ggtcagccac tgctaattca ttttctttca agcctttttg tggtccgaaa acaaaagaag
    11701 cgccttcttt tccgcataaa agattagtaa catcagtaat gacagtgatt ttggctttaa
    11761 tcagctcaat aggcacgttc gttttatcaa ctgtagcaat tttacctaga ttttctccca
    11821 gtgcttctac tttttgatta tcttgatcgt aaaaagcata acctagacct tctgccatgc
    11881 cgatgccgcc atcgttggta ctacttccgc caacgccgat gatgatgtgt cgaacacctt
    11941 gttgcaacaa ttgtcgtatc agttctccta ctcctcgcgt ggataaccta agtggctgtt
    12001 gttggtttag tgggacatct ttcaatccga cgatttctgc catttcaaaa atcgcagttt
    12061 gaccgtctga agcgtactgg atttctttgt ttttgccaaa tgcattagta acgactgctg
    12121 tttgccgctt caaccttaaa ctgtctgcta atgatgaaac cgttccttct cctccatcgc
    12181 ctataggtag cagatcatat tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg
    12241 cttgtgctgc ctctaaggct gtcagacttt ctttgaaaga atctggcgca atcagaatac
    12301 gcattatttt cccctccgtt ccaagatcag ttctttttcc agctggtatt ggtctccatt
    12361 gacaaaaata ggtaatgttt ctttcgtctc tttttttatt actatttgtt tttttgtcaa
    12421 aacaaatagc aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt cccatgctga
    12481 aggaagtctt ggagaaatag aaagagttcc attcgttact gtaatacctg aaaaaccaaa
    12541 tataactgct agccatagtg ctcctaatga agcagcatga attccgtcat ctgatgaatg
    12601 cggatctggt cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg cccattccat
    12661 ttcttggata cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat
    12721 tgttttgttt tcatagtaat gcaaattctt agaaacgatt ggttgagaaa aatactcagg
    12781 taataagtat aacagcatga caagatctgc ttgcttgctg acttggagtt catttacttc
    12841 gctacgggca taatcaagta aaatagactg gctgccagga cgttctttgt aagtggtcaa
    12901 atcaataact ggcttactga gatacgtgtc atcttgcgga atcagctgtt ctttgttagg
    12961 cactggcaaa tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc
    13021 agcttgatat ttaacagcta aggccacatt ataatgcgcc agatagttcg tatatgcatt
    13081 attgtcgata tattctgtgt attcatctgg accaatgact ctgtgtatct ctaaatgatc
    13141 atcgactttt acagctctgc tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc
    13201 ctttgtttct aaaaacgatt gatcctctgt tgcttggaca tactcttgaa cggcataagc
    13261 aaggtctgca ctgatatgat gttctgcggc agctgaagca acttcttgtc ttgttccggt
    13321 tttgatattc aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg cgctttccca
    13381 tgggaaaagt gcaccttgat agtgataatc tcttgctttt tgccgtgcgc cttctaatcg
    13441 atcataacgg tacagcagca aattctttgc tacttcagga aactgatata aaaagaaagg
    13501 caggataaaa atttccgtat cccagaaaac atgcccttta taaccttcgc cagttagtcc
    13561 cttcgcccct acactaacac ttggatcatt gcggggagtc ataatctgta aatggtaagc
    13621 agcgaagtcc atcgctaact gatcgaaaga agaatcgcct tggatcgtta tccgatgctg
    13681 atgccaaaaa tctgtccaat aagacatcga ctgctctaaa atttctgtat aactaacgct
    13741 tttctgggct tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag aggtagagag
    13801 cacgatgatc ttctccagcg taaaggacgt attttttctg atcttttctg aaaaggaagc
    13861 ggtgatccgg cgattttttg cttggaatac acctgcttta ttgaatttag ctgcaatccc
    13921 aatatcaatt ttactttctg ttgtctggta ggatgcactg atcaaatcgt caccaaatac
    13981 tcgcagttct ttttcaacca actgctgtgt accaaaattg gtttgctgtc catcgatacc
    14041 cgttttcaca tctacttggc agtcttcggt caacggcttg atgaccactt tcagggctgc
    14101 ctgatggaga ttattttgat cagcaacacg acaagtaatg acttccactt tttttccttg
    14161 agcagtttca atgagcgcat agcgtttaag ctctcccatt tctaaatcta gtgcccgctc
    14221 gtatgcaaga actttcatcg tctcaagaga aaaatgttca ccgtcgattt ttatttccca
    14281 tctgatgaca tctgggagat tcactaactc ggctgattct gctcctaaag gtcggttata
    14341 tacaccagct gcaaacattc cccttacttg attccgatag tcttcctcct ggcttcctct
    14401 gataccaaaa aatccatttc caatggtcat ctgactcgca tacttattga tatactctaa
    14461 atcaaactgt ttttcttcta ctttcataag gtaataactt ctcctgtttc tgccgaacga
    14521 tataaatttt ccaccagatt ctggatgaca tatccttgcc agccatctgc aatcatctga
    14581 gtctggtcac cgagcgcccg attcacaaaa tgagccatac ttagttcatg tgggtcatta
    14641 actggcgtgg tcttttgtaa ggaaaccagc tttccatcta catcttcgaa tagttcgcct
    14701 ggatacagat tagcacctgc ctgatcgcca caaaaagcca cattcagttt gtcttctctg
    14761 atattcaaag caaacgaagt attcagttgg atgatcttgc cgttttctaa ttccagtgtg
    14821 ccaaaaattg catcttctac tgtgaatttg cttggatccc atttcccgaa tttccctgaa
    14881 ttttttttcg taccaatttt ttggaagctg tacgcccgaa cgctttttac tttaggaaat
    14941 ccagtaatat acatcgctgt atcaagcatg tgaatgccat aatcgatcaa aggcccgcct
    15001 ccttgaagtt ctttgtcgat aaacgttccc cagcctggaa caccagaccg acgaagcgca
    15061 ttcgcttcta cgtaataaat atccccgaga acatcaagat gattcttgat cattgttgct
    15121 tcgtcagaaa aacgatgctg gaatccataa gccaaaatct gcccgttttt atcggctgtc
    15181 tctgccatct ttcttgcttg atcagctgtc atagctggtg gtttctcgca catcactgaa
    15241 gcattagctt cgagggcagc caatacatgt tcgaagtgaa agcgattggc tgtacagaca
    15301 ctcacgatat cgggagaaat ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa
    15361 aatcctgtac gtcggcaaaa gtcttctgct cgctggctga ctggatcaac cacagcggcc
    15421 aattctaaga catctgatct tttttgataa taagcagcat gtactttttc tgctacttgt
    15481 ccagcgccta tgattgctac tttcttcatt tatttctctc tcctttgctt atttacttat
    15541 tgttattctt tctctattct ttctctaaat catttaaatg acgcgctgtt tccagacagt
    15601 ttttcaagta ttgcaatgat tcctgataaa ttttcacttc attttctcct cgcaaacgac
    15661 attcatatac catataacct tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa
    15721 aatcaattgc gccagatcct ggttggtagc gatgattatc tgctaaatgg atatgaccca
    15781 catactcttt ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata ttcatatgat
    15841 aaaaatcagc gatgatctgc acatgtttca attgattaga gtcaatgtaa tgacgcgcat
    15901 ccgctaacaa attgatcata tggtcttggt atcggttcaa aggctccaaa taaataaccg
    15961 tacctgtttc ttcggctact ttatctaaat aacgaagtga ttcgctgact gctttatagt
    16021 ctccttcttt gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac attccccaag
    16081 ctgctgggat aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa
    16141 tttgacgtac accattaaga cgccgttctt cgataaagtc accgatccac ccatcgtagc
    16201 cgttacaagc tgtcactact gggatccctg tttctttgat tgcctgtttg actgtgtcga
    16261 tattttctat taacaactta ccgtcgattt cgtattcttc aaatccacag tctttgataa
    16321 atctaaattt ttctaaaatg tctcttggga aaaatgcttg atcttgcgta ccgaatttca
    16381 ttgttctcct cctcggactt gaacgcccat tttgatactt tcttctgggt gctggtctac
    16441 ataacgcatg taagcttctg cgctttctgc aaatggcaca actggatcaa tgatttcttc
    16501 acaatccaag taaccgttca tcaataattc ccaacatgtg tcctcgattc gtttacggtt
    16561 ccatcttggg taatctgggt ttggttcact agatgctcga gcaaagacga tttttgcatt
    16621 attgaaatgt gcttcgcgac caagattaaa cccagctgga aaaggtttag caaaggcaac
    16681 ataagaaatg atcccgccat agccgatccc tcttaaagaa gcttgtaatg cggcagcact
    16741 gccactcgtt tcaataactg aatctactcc acgtttatct gtcaattcct ttagacgaaa
    16801 accgacatct gctttcatag gatctagcgt gtaatcggca ccgtattttt ctgctaattc
    16861 tctacgctgc gctatgggat cgatcccaac gatggtagta gcacctgctt ttttagctaa
    16921 ttgaatcgca atttgcccga tagcgcctaa accgaagatt gctacagcat cacctgctcg
    16981 aacatgtgct tcccggatac cacctaaagc gaattgagca ggatcgtaac atactgcatt
    17041 tttccattga tccaaccgat caagtttacg tagtttataa ttatcaacac cgtttgtaat
    17101 cagcgtttca cgaatgggtc cataagtgca gactaactct cctttttgat actctgtcac
    17161 caaactgccc gtttcttcga tcgtaccgac aatcatattt cctaattgaa attcgccaaa
    17221 taccacaccg cgttggcttc cttcttctct tggcaagaat aggcgccatt cttctgaaaa
    17281 ttcttcatcg ataaatggac ttaatcctct aaagtcgaca atttctgttc ctggttttgg
    17341 agtagcgaaa attactcgga tacgtacttc gttcttttcg atctcacggt cttcgtaatc
    17401 tttgaatccg gctattcttg gttctaatgc aactaatttt ttcatctttt ctttcctctt
    17461 tcttttcttt ttatcctttt acgccgcctt cgactaaatc acctttaata aatcgttcag
    17521 ataacgcata cataatgatg actggtaaag cggtcaccaa tgaagcggcc atcattcgtc
    17581 cccaaatgta gtctggtgta ctgaataatg tgttcaaacc gattggtaaa gtaaattttt
    17641 cggaggtcga caggaaaatc gaagcaaata aataatcatt ccacgaaacc ataaagcaat
    17701 aaacgaagac agaaatgatt ccagaaatag ccaatggtat aatgatccga ataatgatct
    17761 ggatccggct aagaccatcg atcattgctg cttcttcaat atcagtcgga atcgtgtcaa
    17821 aataactttt tagcatgaat atcgcagtag gaagtgtttg aacgaccatc gtgataatca
    17881 gtgctgtatt ggtatcatac aaacctagac cggaaatgat tttaaataat ggaacaacga
    17941 tcaaaatacc ggaaaacata taaacggtgt aaaagctagc attgattttc gagcgagctt
    18001 tgaagcgtag ttttgacaaa gcgtatgccc cgctgatccc aatcactact gcagttacgg
    18061 aggctatcaa ggagatcact aaactatttt tgaaatacat gatgaatgga aaaatctcag
    18121 cattgaaaat atcctgataa tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg
    18181 ttgcaattgc ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa aatggaaaca
    18241 aattgacagc taatagcaac aaaatcgtcg cgtaaaataa gattttcctg ctctttttat
    18301 ttttcatttc aagtccacct tctttcgtga gagcaagacg accgctgcaa taatgatgaa
    18361 caatagaatc gatatagctg ctgcttttcc aagatcattg aacgcaaagg ctgtctgata
    18421 caaataaacg cctaagatat tgactttatt cgtcagtaga tagacatctg taaacatata
    18481 gaacatccag atagatcgta aaacaatcac agttgctagt actggtttga ttgccggcag
    18541 tgtcacgatc ttgaacttct gccaagcatt tgcaccgtcc atttcagctg cttcataaag
    18601 cgttttgtcg actgtttgca aaatagccaa aaatgaaata aacgcatacg gatagtatct
    18661 ccagatagca aacaaaacga ccataaagaa gctagcaact ggctggtcga accacagcgg
    18721 tgcttcgttg aaaagctgca acctatctac taacagatca ttgacgattc cataactatt
    18781 attgaacata tatttccaag caaaaatcaa agaaatagat ggcgtaacat aacttaataa
    18841 aatcagtgaa cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc
    18901 taatcctaaa gctgtactgc ctgctacaac taagaatgtg taagcaactg ttaaacctaa
    18961 agaacgataa aattcactat cacttaaaat ttcaagataa ttgcttaatc cgataaaaac
    19021 ggcgtctgag ttaggattca gcggcatatc gaaaaaactg atctgaatat tggaaagcat
    19081 tggaatcagc actaaaacag ctaataaaag catgcttggt gctaaaagtc ccatggctaa
    19141 ttttaagtca gaatgtttcc ttggacgatt cattgaaacc tccccctgat aatctgccaa
    19201 tagctatcta cttttttcga acaccattgg cagaacttct tgttatttcg tatttttttg
    19261 tgcgtcagct aaagcttgat cgacatcttc gtttccgaca gttacttggt tcactgcttt
    19321 tccgatcaca cctgagcttg tgatatctcc cattttcgtg aaattctttt ccccgactag
    19381 tccaaagact tgtacttcat caaaagcact agcaatctca gatggcagct cgccaaaaga
    19441 cttgatgact tcattttctt tgtaaccatc cagttcgact acttgtttat tcactggttg
    19501 agcaccacca ggagacatca agacccactt ttccatgttt ttcggttgag ataagaaagc
    19561 tgtgaatgct tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc
    19621 agatactgtg ccatagacag cttgatcttt ttgtgttggg atagcaaaac cgatattttc
    19681 tgagttcccc gcttcataaa cagaaggcaa aatgtaagtt gaatagattg ccattggtgc
    19741 tgttccattc atgaaagcat ctttgatttc tgtcacatca ttagagcctg gcatcgtgta
    19801 ttgggataag ttttgataaa acgacaatgc ttctctcatc ttttccgtat cgatcgttac
    19861 ctcgccttca tcatccaaaa catttgctcc atttgacaaa gcaaattgag agaacgcttg
    19921 ctcactcatt gttccttcag cagtaggcat agcaattcca tattgtttat tctctttatc
    19981 tgtgaaatgt tgagcaattt ttaaaatatc atcccaattt tctggttctg aaaaaccagc
    20041 ttctgataat ttttctttgt cataccaaat accttgaacc cagccgctaa ttggtgcagc
    20101 aatatagctt tttccatctt ctgaacgaac aaggtttttg gctccgtcat aataattatc
    20161 ttctccagct tcctcgataa cgttttggac tgccgtctga tcaatcagtt catctttatc
    20221 catcacttta gcaaaatcct gactgacttc cattacttct ggtaatttac cggaacgagc
    20281 taacgtaacg atttttgtat tgaacgctga ttcttctacc ggcacttgct tgattttgat
    20341 gtctggattt tctttttcaa aatcagcaac cagttcattg atcacactta atcgttcctg
    20401 ttcgacagaa gagtgcatga attgaatcgt tgtctgttgt gattcgttag agctttgact
    20461 ttcaccacaa gcagtaagaa gaaatccagt agctagcaga caggtagtaa caataatttt
    20521 tttcattttt ttccacctct tattttatta ttttcagcca catatattca tacggctgga
    20581 atatcaactt tcgttcttga ctgcaatgga taatttgtcc agaaaacagg tctaggtaat
    20641 ctccttttgg taaaagataa cctgctgatt gattggaaaa attatggata actaagactt
    20701 tctcttcctt cgcttcgcga ataaaactga taaaagaatc tgctgagtcc agtaagtaaa
    20761 tcaaactatt cggatggaag gctttttctt tttttcgaat agatagcatc tgggtcaatt
    20821 caccatatac tgctgttctt aatggatctg tctgcaactc tttctcaata tctgctaaca
    20881 aaaatttttg ccgattgatt tttcgattct ctcctgtttt ttctacgcct ttgtaatcat
    20941 ttcgacttcc cagcacactt tgaatataaa ttgccggcac gcctggtaac gttaccaaaa
    21001 atgaatgagc agctaaaaag cgtttgcttc tccattccgt cgtatctgtt ggagaactta
    21061 acgcatctag atacgtagcg tttatctcat atggactggt cgttccatct gggtttttct
    21121 tgtagtttac tcgggcaccc tctttcttca gctgttccac taattcaagg atcgtttttt
    21181 ccggcagaat acctcgtacc ggattcaatc cgataccgtc atgggaggct aaaaaattaa
    21241 aaaaagttgt ttttttcgat gcgaaatcga tggtttttaa ccattctttc aacttacttg
    21301 tattacccga acgaatcgct tgaagggtca aaggtggtag cgaaaactga tagaccatct
    21361 gtgcttcatt ctcgccattc ccaaaataac tgatattatc tttgtgtggc acatttgtct
    21421 cagtgatcaa taccgttcct ggagctgctt catcagcgat tgcacggaat aattggatga
    21481 ttttatgcgt ttctggcagg tgtatacaag aagttcctag ttctttccat aaaaatccca
    21541 cagcgtccaa gcgaataaat gaagcgcctt tttctaagta aaacaaaaga atatcgatca
    21601 ttttcaataa aacttctgga tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg
    21661 tccatacgtg tttttctttc ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc
    21721 ttggtctagt cacgctgctt gtatctgtat ccagtggcaa atcaataaaa aaatctttgt
    21781 acgtatcatt caagtttaaa tattcctgaa accaagtact ttcggcagac atatgattgc
    21841 agacaaagtc gaacattaac cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc
    21901 ccaaacggtc gttgattgcc tgataattta caacggagaa accatcatca gatgtatatg
    21961 ggaaaaatgg caagaaatgt acaactctaa atgtatctgc caaatacttt tgataaaatt
    22021 tcttaaatgt acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca taagtaatca
    22081 agaaaatatc ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc tgctgataaa
    22141 cactgacttt ttgttcaatc atagaggcgg ttttctcaga caaatctgag tcgcgataaa
    22201 tcgcttgtaa aagttttgta tacatcggat tcccctttct tttttgggag cgttcccaac
    22261 gatttgatca tattatggaa aacgcttact gtcaatagca tttttttaaa taattttgat
    22321 atacacaaaa atacaaataa gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa
    22381 tatggtctct catgatcatg caagctattc ctaaattttc aaaatcatag acaaaaaaga
    22441 atgtctattg tactatggaa ttattctaaa acgctcccaa aaaggaggtg catataaaat
    22501 tgaaacctac tatttatgac attgcgaaaa aagctaatgt ctcaaaatca actgtctcca
    22561 gagtattaaa taaccagcca aatatctctg ataaagcaaa agcaagtgta cttcaagcaa
    22621 tcaaagaatt gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga aatggttttg
    22681 acgcgattct agcattatct catcgtactt ctcatacgac ctatggcaat cctttttttt
    22741 cagatgttat tcaatccatt tctgccgttt ctgaacaaaa taacttcgac ttgattctac
    22801 aaacggcaaa aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa
    22861 tgatccgagg aattattatt ttaagctcta cgatggatga aacacttctt tttgaattgg
    22921 atcaatacaa tattcctatt gtggtaatcg gaaaagtcgg aaacgactac acgaacatct
    22981 actcagtgga cacggataat ttccaggata gttacgattt ggtggaaaca ctgatcaaga
    23041 atggacaccg tcatatcggc tgtctccatg ctccgacaga agtttatgta tccaatgatc
    23101 gtctcaatgg ttaccgtcaa tgcttatttg atcaccacct agatatccgt aatgaatgga
    23161 ttatagacgg cggctttcgc ttagaagata gcatggcagc aatgaatgat ttaatgaagc
    23221 tacgagaaag accaactgcg attttttcta ccgatgcatt aaaatcattg agtatttaca
    23281 aagtggcaga acaatctgga aagcagatac ctgatgatct ttcgatcgca ggattcagtg
    23341 atcccttatt gtcctcccta ttttcgccac agttgactcg gatagacatt ccgacaaatc
    23401 agttaggaat caaagccaca gaacttttat tccaactaat ccaccaccaa taccctgaag
    23461 aaaaaagaat cattctttca accgagatta ctctcacaga ttcgatccaa caacaagaaa
    23521 tgtagaacag aattataaaa gaacagaaac actgaggata agtcagttac tctgttcttt
    23581 tttcaatcac tcatccctcg ttaccccctc ccggtatttt agcgaatttt tcttattttc
    23641 catctgtttt agatagctgt ggataactat aatgtttgtg ttaaactatt ttttccacag
    23701 ggaagaaaaa actattttta accacgttgt gtataagttt ttatagttcg tttttttaaa
    23761 cacatttacc cttatttcaa tccatgttat ttggggatac ttatctctaa aacataaata
    23821 gaataacaga catgtctact ggttttgttt tattcgagga attattcaat aatagctagc
    23881 aaaatattaa aaataaccgc tctatcttcc catcaattta ttttgcattc aaaaaatgat
    23941 ctatctttac ttttgttgat tgacttctct tggaagatga tcgttccgat accttaattg
    24001 gatacgaacc gatgttgtta agatcctttc ccacttttct tctagttaga caaattattt
    24061 taga
//
LOCUS       DAZUJO010000037        23988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000037 DAZUJO010000000
VERSION     DAZUJO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.037"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG8OL_001568"
     CDS             complement(54..788)
                     /locus_tag="ACG8OL_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS4817560.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG8OL_001569"
     CDS             1115..1525
                     /locus_tag="ACG8OL_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS4817561.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG8OL_001570"
     CDS             1518..2219
                     /locus_tag="ACG8OL_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS4817562.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG8OL_001571"
     CDS             2219..3805
                     /locus_tag="ACG8OL_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817563.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG8OL_001572"
     CDS             3824..4045
                     /locus_tag="ACG8OL_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS4817564.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG8OL_001573"
     CDS             4042..4803
                     /locus_tag="ACG8OL_001573"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS4817565.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG8OL_001574"
     CDS             5183..5692
                     /locus_tag="ACG8OL_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4817566.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG8OL_001575"
     CDS             complement(5767..6306)
                     /locus_tag="ACG8OL_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS4817567.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8OL_001576"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG8OL_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS4817568.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG8OL_001577"
     CDS             7319..8170
                     /locus_tag="ACG8OL_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS4817569.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG8OL_001578"
     CDS             8198..8833
                     /locus_tag="ACG8OL_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS4817570.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG8OL_001579"
     CDS             8853..9626
                     /locus_tag="ACG8OL_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4817571.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG8OL_001580"
     CDS             10034..10831
                     /locus_tag="ACG8OL_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS4817572.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG8OL_001581"
     CDS             10809..11222
                     /locus_tag="ACG8OL_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4817573.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8OL_001582"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG8OL_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4817574.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8OL_001583"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG8OL_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS4817575.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG8OL_001584"
     CDS             15999..16559
                     /locus_tag="ACG8OL_001584"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS4817576.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8OL_001585"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG8OL_001585"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS4817577.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG8OL_001586"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG8OL_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS4817578.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8OL_001587"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG8OL_001587"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS4817579.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8OL_001588"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8OL_001588"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS4817580.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8OL_001589"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8OL_001589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS4817581.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8OL_001590"
     CDS             21907..22485
                     /locus_tag="ACG8OL_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4817582.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8OL_001591"
     CDS             22537..23019
                     /locus_tag="ACG8OL_001591"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS4817583.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8OL_001592"
     CDS             23136..23510
                     /locus_tag="ACG8OL_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817584.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7847 a         4311 c         5141 g         6689 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact cctttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtcccttc tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta
    23941 tgaatgattt tactacagaa attgtgcaaa ctctagtcac taaaggcg
//
LOCUS       DAZUJO010000038        23654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000038 DAZUJO010000000
VERSION     DAZUJO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.038"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..602)
                     /locus_tag="ACG8OL_001593"
     CDS             complement(27..602)
                     /locus_tag="ACG8OL_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS4817585.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(607..1581)
                     /locus_tag="ACG8OL_001594"
     CDS             complement(607..1581)
                     /locus_tag="ACG8OL_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS4817586.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1605..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1864..2832
                     /locus_tag="ACG8OL_001595"
     CDS             1864..2832
                     /locus_tag="ACG8OL_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4817587.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2929..3222
                     /locus_tag="ACG8OL_001596"
     CDS             2929..3222
                     /locus_tag="ACG8OL_001596"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4817588.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3340..3576
                     /gene="secG"
                     /locus_tag="ACG8OL_001597"
     CDS             3340..3576
                     /gene="secG"
                     /locus_tag="ACG8OL_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS4817589.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3679..4434
                     /locus_tag="ACG8OL_001598"
     CDS             3679..4434
                     /locus_tag="ACG8OL_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4817590.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4437..6794
                     /gene="rnr"
                     /locus_tag="ACG8OL_001599"
     CDS             4437..6794
                     /gene="rnr"
                     /locus_tag="ACG8OL_001599"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS4817591.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6816..7280
                     /gene="smpB"
                     /locus_tag="ACG8OL_001600"
     CDS             6816..7280
                     /gene="smpB"
                     /locus_tag="ACG8OL_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS4817592.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7582..8301
                     /gene="atpB"
                     /locus_tag="ACG8OL_001601"
     CDS             7582..8301
                     /gene="atpB"
                     /locus_tag="ACG8OL_001601"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS4817593.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8354..8569
                     /gene="atpE"
                     /locus_tag="ACG8OL_001602"
     CDS             8354..8569
                     /gene="atpE"
                     /locus_tag="ACG8OL_001602"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS4817594.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8660..9184
                     /gene="atpF"
                     /locus_tag="ACG8OL_001603"
     CDS             8660..9184
                     /gene="atpF"
                     /locus_tag="ACG8OL_001603"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS4817595.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9171..9713
                     /locus_tag="ACG8OL_001604"
     CDS             9171..9713
                     /locus_tag="ACG8OL_001604"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS4817596.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9736..11292
                     /gene="atpA"
                     /locus_tag="ACG8OL_001605"
     CDS             9736..11292
                     /gene="atpA"
                     /locus_tag="ACG8OL_001605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS4817597.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11306..12208
                     /locus_tag="ACG8OL_001606"
     CDS             11306..12208
                     /locus_tag="ACG8OL_001606"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS4817598.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12231..13637
                     /gene="atpD"
                     /locus_tag="ACG8OL_001607"
     CDS             12231..13637
                     /gene="atpD"
                     /locus_tag="ACG8OL_001607"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS4817599.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13651..14073
                     /locus_tag="ACG8OL_001608"
     CDS             13651..14073
                     /locus_tag="ACG8OL_001608"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS4817600.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14542..14772
                     /locus_tag="ACG8OL_001609"
     CDS             14542..14772
                     /locus_tag="ACG8OL_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS4817601.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14869..16170
                     /gene="murA"
                     /locus_tag="ACG8OL_001610"
     CDS             14869..16170
                     /gene="murA"
                     /locus_tag="ACG8OL_001610"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS4817602.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16183..16359
                     /locus_tag="ACG8OL_001611"
     CDS             16183..16359
                     /locus_tag="ACG8OL_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4817603.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16494..16790
                     /gene="yidD"
                     /locus_tag="ACG8OL_001612"
     CDS             16494..16790
                     /gene="yidD"
                     /locus_tag="ACG8OL_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS4817604.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16854..17321
                     /locus_tag="ACG8OL_001613"
     CDS             16854..17321
                     /locus_tag="ACG8OL_001613"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4817605.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17406..18290
                     /locus_tag="ACG8OL_001614"
     CDS             17406..18290
                     /locus_tag="ACG8OL_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4817606.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18404..18640
                     /locus_tag="ACG8OL_001615"
     CDS             18404..18640
                     /locus_tag="ACG8OL_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS4817607.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18728..18808
                     /locus_tag="ACG8OL_001616"
     tRNA            18728..18808
                     /locus_tag="ACG8OL_001616"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18762..18764,aa:Tyr,seq:gta)
     gene            18815..18886
                     /locus_tag="ACG8OL_001617"
     tRNA            18815..18886
                     /locus_tag="ACG8OL_001617"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18847..18849,aa:Gln,seq:ttg)
     gene            19012..19182
                     /locus_tag="ACG8OL_001618"
     CDS             19012..19182
                     /locus_tag="ACG8OL_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817608.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19402..20109
                     /locus_tag="ACG8OL_001619"
     CDS             19402..20109
                     /locus_tag="ACG8OL_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4817609.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20168..20812)
                     /locus_tag="ACG8OL_001620"
     CDS             complement(20168..20812)
                     /locus_tag="ACG8OL_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS4817610.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20939..21226
                     /locus_tag="ACG8OL_001621"
     CDS             20939..21226
                     /locus_tag="ACG8OL_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817611.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21334..21654
                     /locus_tag="ACG8OL_001622"
     CDS             21334..21654
                     /locus_tag="ACG8OL_001622"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4817612.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21735..23048
                     /locus_tag="ACG8OL_001623"
                     /pseudo
     CDS             21735..23048
                     /locus_tag="ACG8OL_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23045..23464
                     /locus_tag="ACG8OL_001624"
     CDS             23045..23464
                     /locus_tag="ACG8OL_001624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817613.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKRLPGIYLLSNTFI"
BASE COUNT         7820 a         4125 c         5092 g         6617 t
ORIGIN      
        1 tattttgcta atccagctct gtttattcag cctatttttc tttttgaata tatcaagcgt
       61 tgtggcaaca tagtgatgac taatgaaaca acgacgatag acaacaaacg atcaaggtag
      121 tctgtacctg cctgtaccaa gatcacactt gttgtctgac ttaaacctaa cccatgtaat
      181 agctgaacga tcatactgga tcctgaggag gtgatcccgc caaataactg aacggtaatg
      241 atcgaagaaa cgatcgttcc aggtaccgac aaaatcaaag cgagtaataa cagtttcaag
      301 ctgtttttat ctttgccgag atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga
      361 atttggattg gcgaataata cagagaaaaa atatctgtag tcactccact taaaataccg
      421 ctggacaatc ctgtcagcat tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt
      481 ccgatagaat cgagatagat tggcaaacga agccataaag caatcgttcc tccaatataa
      541 ttgatcccaa tacacaaagc catcactgta agtacgcgag ttgccatttt gtttttattc
      601 attttgttag cccatcttta acctttccaa atctttcata atcgtttctt cttgtgcatt
      661 taaaagaacg gttaaaaact ttcggaaaaa aagacgagta tctacttttg tcataatttt
      721 actgttagca ggtttctgcc aaaattcata acgatcaacc aatgtttgtc cacgacttat
      781 tccttgagtt tccacagcag tgaagctttt gaaaccttca cagatggcct cctcgataaa
      841 ataagcaaca gctaacggat cattgatgac acatcctaaa attctttctt gtttccagtg
      901 gaagtcaaaa taaaaacgag tgatcgcttt tagatactct ccctcttcag gattcatttg
      961 acagcagtat tcgaggatag tcggtgtcaa gacgatttct cttgtcacat ctaacccaac
     1021 catttctatc gtttgtttga gattctcgaa tacataagaa gctgcttcag gatcacacca
     1081 gtaattgtat tcagcaactg gcgaacagtt accatggctt ttgtaagttc cacccatcga
     1141 gacaaaacgt tccatatgtt tcccaatatt tgggttgact ttcaaagcag aagcgatatt
     1201 ggtcaaagga cccaaagcaa tgactgatgt atttgtcttt tctttgaaat aatcagccaa
     1261 aaaatcaacg gcatggagag gttctgcctg ttttctcaag atcatcggga aatttgtttc
     1321 ccctaaacca tccataccat gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt
     1381 ttccattcct tgataaacag gaatgtctaa acgatcacag cgttccaggc atttcagcac
     1441 attctgcgta cctatatctg cagggacatt cccacaaact acggtaatag caactacttc
     1501 gatatcaggg gatttgacag ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca
     1561 atcaataatt acttttcgca ttatttccaa tctcctttct ttagagatac ttagtttttt
     1621 attctgggaa gtttccgaac cagtccaaga cgagcgtctt gattataatg cacgaaatta
     1681 gtatactggc ttttattgcg aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa
     1741 ctttaagtaa atgatacaat gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat
     1801 cctatggatc ggtacattgg ataatagata gattatagtt tggcagaaag cggcgggtgt
     1861 acaatggtta ttttttattt cattcttatt ctgataatta tagggatcag ttaccatttt
     1921 ctttcttatt tttatgcaaa gaaattagta agagcgggag tacagagagg atatgactgg
     1981 tatgaagaca ctggtcatcg tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa
     2041 cgacaaaaat tagaagaaga gtgtgaaagc ttttgggagc agggaacacc caaaacaatc
     2101 aaaagttatg acgggttgaa actagctggt cagatgttca tccatccaaa taagcagaat
     2161 aaatgggtca tttgcgtaca tgattaccga agcacaggga aaagagacat gtctcatata
     2221 ggtaaaagat atgcagagaa aggattcaac gttcttgtac ctgatttacg tgcgcacgga
     2281 gaaagtgaag gagaaatcat cggaatggga tggctagatc ggctagattt aattgcctgg
     2341 atccaactca tcttagatga acagccgaat gcgtccatta ttttacacgg cggatcaatg
     2401 ggagcttcta cgatcatgat ggcaagtggt gaaaaacttc catcagcggt caaaggattt
     2461 attttagaca gcggatatgt ttctgtttat gcagaattcc gatatatgtt aagtaaaatc
     2521 actgtctttc ctaaaaaaat ggtcatgaga tatgcgaatc attatgccca gaaatacgcc
     2581 ggttactcct taaagcaagc atcagctacg agacagcttg gcagcaatca tctgccttta
     2641 ttagtcatcc atggtgaaag agatcatttt gtccctacag aagcagcgta cacgattcaa
     2701 aatgctacgg caggggataa agcactgctg cttgttccgg aagcagaaca cttggaagca
     2761 tcgatgaaag atccgaaaac ctattggaca gtgatttttt catttattga acaaagaata
     2821 acgatgaatt aattttctct tcttttttgt aagtaattcg aggataaata attgtttata
     2881 gatcaggaga ctgctaaaat aaggtaggaa taaaaaaata tcagttattt gaggagtgcg
     2941 gcatatggaa atcaaagaag aaaaaatcgt cttgctttat tcaatgatga acaacaagag
     3001 atcggagaaa tgacatggtc agatgcgggc ccagacatca tgatcgttga ccatacattt
     3061 gtagatccag catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa
     3121 ttagcaagac gagacgggaa aaagatcatt cctttatgcc cttatgcaaa aaaagagttt
     3181 gaaaagaaac cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt
     3241 ttgaactttc agctgttatt ccatttatat agagttgtca cggactttta tctatgataa
     3301 agttaaagag acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga
     3361 atcgtgatcg ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa
     3421 aatagtgcag cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct
     3481 cgcggctttg aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc
     3541 ttattatttg tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg
     3601 cgggcggctt tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg
     3661 taaaatgagg cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa
     3721 catggaaagc gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga
     3781 atgctcgcac gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga
     3841 cacggaacaa tggagccata tgatatcttg caagaaaaag cagaaagctg gtgggcagac
     3901 actaaaaaag ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga
     3961 ttatcaatgg gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc
     4021 ggttttttct gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa
     4081 tacgtacgtc aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca
     4141 gttgcttacc gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc
     4201 gtagaaagta ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat
     4261 gaaatgatcg accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg
     4321 acacttcaat ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa
     4381 ttagaaaaag acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga
     4441 caaaagaaac gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt
     4501 tttctatgga agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac
     4561 tagtacaaac gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa
     4621 aagtgaaact tcctttaaaa ccaatcctga tcgaaggaac atttcgagca aatgaacgag
     4681 gatttggatt tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga
     4741 ctggttttgc aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag
     4801 atatggatcg cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt
     4861 tagcagggga attcgttgct tattcagaag aagaaataca agaaacagat ctttacggcg
     4921 ttgttatccc gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag
     4981 ttcgtccagt tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt
     5041 atgcgactag tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca
     5101 tggatatcct ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat
     5161 tgtctgaagc agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag
     5221 atttaagaaa tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg
     5281 cagttacggt taaaaaatta gataacggta attattttct tggagtacac attgcagatg
     5341 tttcttacta tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa
     5401 gtgtatattt gactgatcga gtaattccga tgatcccgca acgtttatcg aatggtatct
     5461 gttcactgaa tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa
     5521 atggacaagt agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga
     5581 cctacacagc tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg
     5641 ctcagctcgt acctatgttc caagaaatgg gtgaattgca ccgaatcctt gaaacgatgc
     5701 gaatcaaacg tggcgcgatt tcttttgagg atcgcgaagc gaaaatcctt gttgatgctg
     5761 aaggacaccc aaatgacatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat
     5821 ctttcatgct tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt
     5881 ttatttaccg aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg
     5941 cttcagcttt gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac
     6001 agcatgtgat cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc
     6061 taagaagcat gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg
     6121 cagattacta cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc
     6181 gtctgatccg ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctgcgg
     6241 aacttcctga aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac
     6301 gtgaagtaga ttccatgaag aaagcagaat tcatgatgga caaagtggga gaagaattcg
     6361 atgggatcat cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag
     6421 agggattgat ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt
     6481 tagctcttgt aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa
     6541 aagtagtcaa agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag
     6601 aggttacagc gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa
     6661 aacaagcagg aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta
     6721 agcaaacgtt cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga
     6781 aaaagaaaaa ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa
     6841 ttgcacaaaa caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga
     6901 tggtcttgca aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg
     6961 gatttattcg cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg
     7021 aacaaggaaa tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa
     7081 aacaaatcat ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa
     7141 aagtctatat tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat
     7201 cttatgataa acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat
     7261 taaaaaattt ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt
     7321 acttaatgat aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt
     7381 cttagatcag gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa
     7441 agtgtctaat tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg
     7501 tggttagaaa ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc
     7561 ggggaaaaga ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt
     7621 tggtttgatg gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta
     7681 gcttactttt ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa
     7741 tgggtcattg attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc
     7801 aatttccatt tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt
     7861 ttagtaacaa aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac
     7921 ccgtttgtta ctttaacttt ggcatttata atgatcttac ttactcactt attcggggtg
     7981 aaaaagttag gcttcaaagg atatttcgta aattcgttct tgaaacccta tagttttatg
     8041 tttccgatga aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat
     8101 gggaatattt atgcggggga agttttgcta accttgattg caaaaatgat gacaagctta
     8161 ggctggttct cattaccatt agcaattcca ttagagatgg tctggatcgc gttctccttg
     8221 tttattggga gcatccaagc atttgtcttt gtcactttgt caatggttta tatgagccat
     8281 aagattgaag tagaagaata atactcggtt aaaaaacaaa ctactattta tctattttag
     8341 gaggaaaaca attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg
     8401 agccggttat ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc
     8461 tgaaatgtct ggtcaattcc gtacaacaat gtttatcggg gtagctctag ttgaagctgt
     8521 tcctatctta ggggtagtta tcgccttgat tctagttttt gccgtttaat tgaatcagta
     8581 ttaaggcaga aacaacatcg tttgttactg cttttttaca tggaaactag aaatactcca
     8641 aagaaaggaa ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat
     8701 gttaggtaat atcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca
     8761 ctttgcttgg ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga
     8821 tttagattct gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca
     8881 acaattgtta gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg
     8941 agaattaagc cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag
     9001 caaagcccaa gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga
     9061 cgttgcagaa ctctctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga
     9121 aatgcatgaa tcattaatca atcaatacat tgaaggtcta ggttcttcaa atgaaactag
     9181 ataaatatac agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa
     9241 atcaagcaga agaaatctat caagatctcg taagtttacg tcaaatctat gaacaagtac
     9301 ctggattagg gaatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca
     9361 tggacaagct tgtcagcggt tatgatggga tcgttaaaaa ctttttagaa gttgtttatg
     9421 agtataaccg aatggatgac cttttgttca tgatcgaaga atatgaacgt cgatatgatg
     9481 aacatcaagg gttactattg ggaagtgtca caacagcagt cccactttca gacgaacaac
     9541 gaatacaatt agaaaagaat gttgcaaaaa caatggacta tcaatcagtt gaattaaaac
     9601 aaatcgttga ttcttctatt attggtggcg cgattgttga agccaaccat cgagtaatcg
     9661 acggaagcat tcgcacgcaa ttagaaaaaa tgcgtaatca attaaacaga tagtacaaag
     9721 aggtaggtga atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa
     9781 attgaaaatt atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt
     9841 gacgggatcg ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt
     9901 tcaaacggct catacggaat ggcgcaaaac ttagagtcaa atgatgtagg tatcattatc
     9961 cttggcgatt tcgagtccat tcgtgaagga gataaagtaa aacgaacagg taagatcatg
    10021 gaagttccag ttggagaggc cttgatcggt cgggtagtca atccgctagg tcaaccaatc
    10081 gacggactag gtgaaatcgt tactgacaaa gctcgtccag tagaagcgat ggcaccaggt
    10141 gttatgcaac gtaaatctgt taacgaacca atgcaaacag ggctaaaagc gatcgacgcc
    10201 cttgtgccaa tcggacgcgg acaacgtgaa ttagtcatcg gtgaccgtaa aacagggaaa
    10261 acttctattg cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat
    10321 gtagcaatcg gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat
    10381 ggcgcaatgg attacacaat cgttgtgaat gccggtgcgt ctcaaccagc accattgctt
    10441 tatatcgcac catatgctgg tactgcaatg ggtgaagaat tcatgtacaa cggtaaacat
    10501 gtattgatca tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg
    10561 ctacttcgcc gtccaccagg tcgtgaagct tatcctgggg atgttttcta cttgcactca
    10621 cgtttattag aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc
    10681 ttaccatttg ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt
    10741 tcgatcactg atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca
    10801 gcggttgatg ccggactatc tgtatctcgt gtaggaggtt ctgcgcaaat caaagcaatg
    10861 aaaaaagttg ctgggacttt acgcctagac ttagcaagtt accgtgaatt agaagcattt
    10921 acacaattcg gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt
    10981 acagtagaaa tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg
    11041 attttgtatg ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac
    11101 ttcgaacatg aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc
    11161 cgtacaacaa aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa
    11221 gacatgttct tagctacaaa aggaagtaat tcctctacag aagataaatt gaaatccatt
    11281 caaaatgcat agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat
    11341 cgcttcaacg aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa
    11401 gttgacaaaa tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga
    11461 aatcgttacg catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg
    11521 ggatatcaat tacaacagca tgctgctcag ccgtcctgtg aagaaaactg gttatatcgt
    11581 catcactgca gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat
    11641 gtctatttta gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg
    11701 aactggtgca gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct
    11761 atctgatcag ccaagctttg atgaagtccg caaaattgta aatatggcaa caactatgtt
    11821 ccaaaacgaa gtattcgatg aattatacgt atgttataac catcatatca attcgctaac
    11881 tagtcagttt cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac
    11941 gactttcgaa caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt
    12001 gcctcagtat gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca
    12061 tgctgctggt atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga
    12121 gctgacggtt tcttataacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat
    12181 tgttgccgga gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag
    12241 gcaagattgt tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct
    12301 taccagatat caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg
    12361 ttcttgaagc agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa
    12421 cagatggttt acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg
    12481 taggtaaaga aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa
    12541 cacctttccc agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg
    12601 aattaagtac aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc
    12661 catatttaaa aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac
    12721 tgatccaaga actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg
    12781 gtgtaggtga acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag
    12841 ttatcgaaaa aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc
    12901 gtgttgcttt gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg
    12961 tactgttgtt tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct
    13021 tgttaggacg gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc
    13081 aattgcaaga acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct
    13141 atgtgccagc cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg
    13201 cgacaaccaa cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat
    13261 tggcttcttc ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag
    13321 caacagaagt ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct
    13381 taggtatgga tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc
    13441 aattcttctt gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat
    13501 atgtccctgt agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg
    13561 atcttcctga agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga
    13621 aaacactagg ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta
    13681 actcctgccg gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct
    13741 ggtgagcttg ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa
    13801 gttcgtgtaa aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga
    13861 atcatggaag ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt
    13921 gatatcgatg tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa
    13981 gcaaaagcaa aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca
    14041 atcaatcgta tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt
    14101 taagaacagt aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt
    14161 tacgaaaatt gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt
    14221 ctggaacacc gtatatcaga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac
    14281 aaatggaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga
    14341 ttgaacaccg tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt
    14401 ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta
    14461 tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca
    14521 atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg
    14581 agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact
    14641 tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt
    14701 ttagggtaca ctgcaagtaa ttttttctta gaggttatcg ctttatgccg aaatcttttc
    14761 gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa
    14821 agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc
    14881 atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct
    14941 gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac
    15001 gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat
    15061 gtagattttg atgaacaaaa gaatcaagta acaatcgacg cttcaagaca attagaaatc
    15121 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
    15181 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
    15241 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
    15301 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
    15361 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
    15421 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
    15481 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
    15541 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
    15601 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
    15661 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
    15721 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
    15781 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg
    15841 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
    15901 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
    15961 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
    16021 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
    16081 catatcaaac tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct
    16141 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
    16201 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
    16261 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
    16321 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
    16381 ctagtcgata tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt
    16441 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
    16501 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
    16561 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
    16621 cgctaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
    16681 gcggtatcga ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag
    16741 aaccagatta taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
    16801 gaaatttatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
    16861 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
    16921 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
    16981 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
    17041 taaccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
    17101 agctttcaca gctagcttca aaccaaactc ggatcattat acgtactggc actactagta
    17161 gcgctgcgtt acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
    17221 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
    17281 tgattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
    17341 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
    17401 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
    17461 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
    17521 aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
    17581 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
    17641 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
    17701 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
    17761 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
    17821 cgaaaggaca aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct
    17881 tagatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
    17941 gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag
    18001 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
    18061 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt
    18121 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
    18181 tcgatctgca tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc
    18241 ttataggtgc agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga
    18301 gaaggtgcag cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa
    18361 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
    18421 taaagatatt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
    18481 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
    18541 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
    18601 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
    18661 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
    18721 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
    18781 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
    18841 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
    18901 aagctctcgt tagttcgaga gctctttttt tttgctttgc taccttgttc cttgaagatt
    18961 ctgctgaatt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
    19021 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
    19081 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
    19141 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
    19201 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
    19261 aaaattgttt ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg
    19321 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
    19381 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
    19441 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
    19501 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
    19561 ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa
    19621 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat
    19681 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
    19741 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
    19801 gatgatgtcc ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac
    19861 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
    19921 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
    19981 ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
    20041 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
    20101 gaaaaataaa aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca
    20161 cctgttttta tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa
    20221 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtta gcactttttt
    20281 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
    20341 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
    20401 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat
    20461 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
    20521 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg
    20581 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
    20641 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaat
    20701 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
    20761 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
    20821 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
    20881 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
    20941 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
    21001 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
    21061 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
    21121 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
    21181 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
    21241 gaaaacgttg acaaatttct ttctttttat ataataaagt aacagacgcg aagaaaacgt
    21301 ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta aaaaaacaat catgcttgta
    21361 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
    21421 aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
    21481 aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt
    21541 gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt
    21601 atgatgaatg gcgaaaaagt gttagatcaa gcaatcgcgc tcataggtga ataaatagaa
    21661 cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt
    21721 ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag
    21781 caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt
    21841 tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt gttggacagc
    21901 ttgcagatta cggttatttt tgtacacatt gtctatattt agggtgatgc gacactcaac
    21961 aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga
    22021 caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc cattcagaaa
    22081 aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta
    22141 ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg
    22201 tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc
    22261 tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc
    22321 agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc
    22381 cttataacgg ggatgctggg ctagctcacg ctgtgcaaac agcaatcaga tcaaacggcc
    22441 gattcatcta cttaagtgcc acgcctgatg agaagttact aaaggaaatc aagcagacat
    22501 ttgagctcca caaacttgcc ttgcgctttc atcggcgctt gcttcctgaa cccgtgcttg
    22561 ttttcatgaa taattggcga caaaaatgtg gaaacaaaaa gaaaatcaac tctttggtgc
    22621 ggatcattca gcaactaatc aaagaaaacc gtatcctttt attttgtcca agtattacga
    22681 tgatgcatcg tttaaaaatc acactgcaag aagcttttcc agaatatgag ataacagatg
    22741 tttcttcaca agacgatcaa cgggcaaaga aagttcagaa aatgcgagaa gcaatatacg
    22801 agatactgtt aaccacaatg atcctagaaa gaggagtcac atttgaaaga atctctgtca
    22861 tcgttttagg agcagatcat ccagttttta ctaaatcctc tttggtgcaa atcgcaggaa
    22921 gagctgatcg taaaggtcct tatacgaaca gtcgagtcta ttttttctat gaagaaaaaa
    22981 cttcagctat tcgaaaagcc tgtcaggaaa tcaaagaaat gaacgaagaa gggaagcaga
    23041 tgctgtgaga tgtagttgct gcggtaaaga catcattcaa aatttaacat tcagcgagat
    23101 catcggagtg tcgaaatcta gacttcctca gacatgttca gtttgccgcg cacaattcca
    23161 tttattagaa gcaaaaggtt gtgcaggttg tcaaaaacca atccatttga aatacgagga
    23221 gacgaaaaag aaagcaaata ctagtttcgt ttactgcaac gactgtcaag aatggaagaa
    23281 gaaatatcct gcatatgatt ttcaacaccg cgcctttttt acgtacgatg aaaagatgca
    23341 ggcttggatc aatcggtaca agtttatggg agatatccga ttagcaggga cttttgcata
    23401 taaatggact cagataaaaa aaagattacc cggcatatat ttattgtcca atacctttat
    23461 ctgaaaaaag gtggctagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa
    23521 aaattccttt tcagatgctg ctcaaaagag agcaacatct gcttgctcaa gcaaaaaaca
    23581 cgcgaaagga aagattggag atgcctcagc cttttgagct tgccgtacct ctttctaaga
    23641 tacaacataa aaaa
//
LOCUS       DAZUJO010000039        23653 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000039 DAZUJO010000000
VERSION     DAZUJO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.039"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..506)
                     /locus_tag="ACG8OL_001625"
     CDS             complement(<1..506)
                     /locus_tag="ACG8OL_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4817614.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="ACG8OL_001626"
     CDS             779..3250
                     /locus_tag="ACG8OL_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4817615.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="ACG8OL_001627"
     CDS             complement(3353..5089)
                     /locus_tag="ACG8OL_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4817616.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="ACG8OL_001628"
     CDS             complement(5086..5790)
                     /locus_tag="ACG8OL_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4817617.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="ACG8OL_001629"
     CDS             5921..6424
                     /locus_tag="ACG8OL_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS4817618.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="ACG8OL_001630"
     CDS             6384..6722
                     /locus_tag="ACG8OL_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS4817619.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="ACG8OL_001631"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="ACG8OL_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS4817620.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="ACG8OL_001632"
     CDS             8273..8851
                     /locus_tag="ACG8OL_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS4817621.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="ACG8OL_001633"
     CDS             8855..9376
                     /locus_tag="ACG8OL_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS4817622.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="ACG8OL_001634"
     CDS             complement(9455..10009)
                     /locus_tag="ACG8OL_001634"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS4817623.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="ACG8OL_001635"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="ACG8OL_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS4817624.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="ACG8OL_001636"
     CDS             10549..10800
                     /locus_tag="ACG8OL_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS4817625.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="ACG8OL_001637"
     CDS             10925..11716
                     /locus_tag="ACG8OL_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817626.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="ACG8OL_001638"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="ACG8OL_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS4817627.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="ACG8OL_001639"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="ACG8OL_001639"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS4817628.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="ACG8OL_001640"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="ACG8OL_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS4817629.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="ACG8OL_001641"
     CDS             14014..14676
                     /locus_tag="ACG8OL_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817630.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="ACG8OL_001642"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="ACG8OL_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4817631.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="ACG8OL_001643"
     CDS             18096..18410
                     /locus_tag="ACG8OL_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4817632.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="ACG8OL_001644"
     CDS             18423..18797
                     /locus_tag="ACG8OL_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4817633.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="ACG8OL_001645"
     CDS             18807..19151
                     /locus_tag="ACG8OL_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS4817634.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="ACG8OL_001646"
     CDS             19222..20574
                     /locus_tag="ACG8OL_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4817635.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="ACG8OL_001647"
     CDS             20634..21776
                     /locus_tag="ACG8OL_001647"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS4817636.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="ACG8OL_001648"
     CDS             21801..22055
                     /locus_tag="ACG8OL_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817637.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /gene="mobT"
                     /locus_tag="ACG8OL_001649"
     CDS             22311..23495
                     /gene="mobT"
                     /locus_tag="ACG8OL_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4817638.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23492..23629
                     /locus_tag="ACG8OL_001650"
     CDS             23492..23629
                     /locus_tag="ACG8OL_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4817639.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7832 a         4219 c         5073 g         6529 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacattg
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgaccaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DAZUJO010000040        23580 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000040 DAZUJO010000000
VERSION     DAZUJO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.040"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..129)
                     /locus_tag="ACG8OL_001651"
     CDS             complement(10..129)
                     /locus_tag="ACG8OL_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS4817640.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            308..1969
                     /locus_tag="ACG8OL_001652"
     CDS             308..1969
                     /locus_tag="ACG8OL_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4817641.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2039..2386)
                     /locus_tag="ACG8OL_001653"
     CDS             complement(2039..2386)
                     /locus_tag="ACG8OL_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS4817642.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2470..3411
                     /gene="opp3b"
                     /locus_tag="ACG8OL_001654"
     CDS             2470..3411
                     /gene="opp3b"
                     /locus_tag="ACG8OL_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS4817643.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3412..4455
                     /locus_tag="ACG8OL_001655"
     CDS             3412..4455
                     /locus_tag="ACG8OL_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817644.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4461..5531
                     /locus_tag="ACG8OL_001656"
     CDS             4461..5531
                     /locus_tag="ACG8OL_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817645.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5521..6474
                     /locus_tag="ACG8OL_001657"
     CDS             5521..6474
                     /locus_tag="ACG8OL_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817646.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6486..7496
                     /locus_tag="ACG8OL_001658"
     CDS             6486..7496
                     /locus_tag="ACG8OL_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS4817647.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7781..8602)
                     /locus_tag="ACG8OL_001659"
     CDS             complement(7781..8602)
                     /locus_tag="ACG8OL_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS4817648.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8699..8977)
                     /locus_tag="ACG8OL_001660"
     CDS             complement(8699..8977)
                     /locus_tag="ACG8OL_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817649.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9245..11254
                     /gene="metG"
                     /locus_tag="ACG8OL_001661"
     CDS             9245..11254
                     /gene="metG"
                     /locus_tag="ACG8OL_001661"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS4817650.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11733..12320)
                     /locus_tag="ACG8OL_001662"
     CDS             complement(11733..12320)
                     /locus_tag="ACG8OL_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817651.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12495..13817
                     /locus_tag="ACG8OL_001663"
     CDS             12495..13817
                     /locus_tag="ACG8OL_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4817652.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13972..14748
                     /locus_tag="ACG8OL_001664"
     CDS             13972..14748
                     /locus_tag="ACG8OL_001664"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4817653.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14741..15310
                     /gene="rnmV"
                     /locus_tag="ACG8OL_001665"
     CDS             14741..15310
                     /gene="rnmV"
                     /locus_tag="ACG8OL_001665"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS4817654.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15303..16187
                     /gene="rsmA"
                     /locus_tag="ACG8OL_001666"
     CDS             15303..16187
                     /gene="rsmA"
                     /locus_tag="ACG8OL_001666"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS4817655.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16638..17267
                     /locus_tag="ACG8OL_001667"
     CDS             16638..17267
                     /locus_tag="ACG8OL_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817656.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17391..18500)
                     /locus_tag="ACG8OL_001668"
     CDS             complement(17391..18500)
                     /locus_tag="ACG8OL_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817657.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18709..19131
                     /gene="mgsA"
                     /locus_tag="ACG8OL_001669"
     CDS             18709..19131
                     /gene="mgsA"
                     /locus_tag="ACG8OL_001669"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS4817658.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19318..19416
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19454..19990
                     /locus_tag="ACG8OL_001670"
     CDS             19454..19990
                     /locus_tag="ACG8OL_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS4817659.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20030..21793)
                     /locus_tag="ACG8OL_001671"
     CDS             complement(20030..21793)
                     /locus_tag="ACG8OL_001671"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817660.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21796..23511)
                     /locus_tag="ACG8OL_001672"
     CDS             complement(21796..23511)
                     /locus_tag="ACG8OL_001672"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817661.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7591 a         4318 c         4788 g         6883 t
ORIGIN      
        1 accgtttatt cagataaggg actgtgacaa gacttttgtc acagtttctt tttgtttatt
       61 cttttttatc tttgtatggc aataatagac aatataacga aaattattta atggcttttg
      121 gaagaccatg gaaaaccttg tacaaagcta ttttttctga aaaattcctt gaaacctcac
      181 gaaaaaatca ttattttatg aaaaagacaa aaaaaagtgt tgatttttta ataattattc
      241 attaaactct taaacaactt cagaaaatat taattattct gaaaattcca ataaaggggt
      301 acgtaaaatg aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc
      361 tggatgttat ggcggcagtt cagccacaag cagtagttca gcaaaaagcg gttcagcaga
      421 tggaggaggc gtatttaact tagtcgtgcc tcaagaaatg cctacagctg acttatcagt
      481 tgcaacagat acaatcagtt ttacagcatt gaataacgtt tatgaaggaa tttaccgtct
      541 tgatgaagac agcaaaccac agccggcagg agctagcgag ttggcagaag tcagtgaaga
      601 cggattaact tacaaaatca aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt
      661 tacagcagcg gattacgttt atggctggca acgaacagta gacccagcta cagcttctga
      721 atatgcttac ctgtatgcgc cagttgaaaa tgcagaagac attacagctg gaaagaaaga
      781 taaatcagaa ctagggatca aagcagttgg tgactatgaa ttagaaatca aattgacgaa
      841 acaaacacca tatttccaat atctgcttgc tttcccatca tttttcccac aaaaacaatc
      901 cgtagtggaa gaaaatgggg atgcctatgc atctgcaagt gataaagctg tttataatgg
      961 accattcaca ttagaagatt ttgatggacc aggaacagat acagaatgga cttataagaa
     1021 aaatgatcat tactgggaca aagatacagt aaaactttct gaaatcaaag tcagtgtagt
     1081 aaaagaatct tctactgcat tgaacttatt caaagatggc caagcagatg acgtgatctt
     1141 gtctggtgag ttagcacagc aaaacgcaaa cgatccagct tatacttctg ttaaagaagc
     1201 gcggacaagc tatatcgaat tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa
     1261 cctaagaaaa gctatttctt attctatcga tcgtgaagct ttagtaaaac aagtactagg
     1321 tgacggatca gttgtctcta caggattgat cccatcagat atggtaaaaa gtccaacaac
     1381 gaacgaagac tttgcaaaag aagctggaaa attagtttct tatgacaaag ataaagcaaa
     1441 agaatattgg gaaaaagcga aaaaagaatt gggcgttgat tctttagaat ttgatttgat
     1501 ggcttctgat gatgattcat cgaaaaaagt aatcgaatat gttcaaaact caatccaaga
     1561 aaacttggat ggcgtaacag tcaaaccaac acctgttcca ttctctgtac gtcttgaccg
     1621 ctcaacttcg ggtgattttg acgcagtact aggcggatgg gctgctgact atgcagaccc
     1681 aagcagcttt actgatttgt ttgtcacagg taattcatac aaccgtggtc gctggagcaa
     1741 tgcagaatat gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc
     1801 ccgttgggat gatttccaaa aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc
     1861 ggtgtaccaa aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca
     1921 tgcggcaggc gcttcatggg attacaaatg gacatacgtt gaagaataat cgttaatacc
     1981 gtgagcatga gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc atattcactt
     2041 atcctaacaa agaaaaaagc atgaaaatag cagaaagagc aattagtatt ccggcaatga
     2101 tcagccaaaa gctgtacata ccgtagctgg cagacgagag cgtggaaaat tctggttttt
     2161 tctttcggtt ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg
     2221 aagaaaagac ccataaaaaa ccgccgataa tcagaaacgg caatgaccat aagaatagat
     2281 cattagataa ttgaatgagt gtaaaggaat ggttgatgag attttttatt aaaataacca
     2341 gaatcagtag aaaagctact gcgtagggga tagatttttt tttcatttga ttgctccttt
     2401 atttttggtt cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg
     2461 gaaacattag tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc
     2521 acgctttggt taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca
     2581 tatacgaatc aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt
     2641 ttggataaac cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat
     2701 tttggaattt ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc
     2761 ggaccatcct tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt
     2821 ttaggaacga tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta
     2881 gcgatattag gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt
     2941 gcaatcaaat tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta
     3001 ccaacaattg cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa
     3061 atggtggaag tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt
     3121 tgggaaatcg cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta
     3181 ggaccgttag ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt
     3241 cctggtatcg gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg
     3301 gctgtaacga tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg
     3361 tacggtctag ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg
     3421 ataccaaagc gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa
     3481 aaaaataccg aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat
     3541 tcatggcgcc gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc
     3601 atcattttga tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac
     3661 gtggcttata tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga
     3721 aaagcaatgg ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac
     3781 gtaaatttct ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg
     3841 ggaatgagaa tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt
     3901 gtagcttacg ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt
     3961 tttttggaaa tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt
     4021 tttgaaccag gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg
     4081 gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga
     4141 ttgacattag gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct
     4201 agtgtcatta ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt
     4261 ttaagcttta tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc
     4321 gaagggtaca agacattcct gtatctgcct tatctattat ggattccggc agtaacttta
     4381 tcggttatca tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct
     4441 aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt
     4501 ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag
     4561 gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca
     4621 tcatgcgctt gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta
     4681 acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga
     4741 tgattttcca agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg
     4801 ctgagtccct tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag
     4861 aattgcttaa attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc
     4921 agttctcagg aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg
     4981 aaatcctgat tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt
     5041 tggagctgct gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg
     5101 accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg
     5161 ttgaagttgg aacatcggaa gaaattttct acaacccgca acatccctat acttggggac
     5221 tgcttggttc aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt
     5281 ctccaccgga tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg
     5341 cgatgaaaat cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg
     5401 cagcaacttg gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac
     5461 gtcgctgggc tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg
     5521 atgccaaata aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag
     5581 aatgaagtac atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt
     5641 ttagttggag aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat
     5701 gaacctactg atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa
     5761 aaaggtttga cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg
     5821 ttgaatcctc gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta
     5881 gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg
     5941 aatccaagcc acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt
     6001 ggtattgcac gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct
     6061 gcactagatg tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa
     6121 catcaattga cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac
     6181 cgaatcggtg taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat
     6241 aatcacggtg ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca
     6301 gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta
     6361 cgtcaacttc gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca
     6421 tattacgcaa aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga
     6481 catagatgct aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat
     6541 tgattcgtac ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga
     6601 aaagttggca acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag
     6661 aatacccatt cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg
     6721 atcgggcttt tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa
     6781 aaagtatgtg gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg
     6841 gctacttaaa gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg
     6901 aaggatcgat tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac
     6961 tactttgtga tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct
     7021 atcatccgaa gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc
     7081 accagacaga agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg
     7141 ctcaccatag aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag
     7201 taatggatct ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg
     7261 ggaaattggt caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac
     7321 atgctgattt ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc
     7381 aatggtataa taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg
     7441 taagaaagag ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa
     7501 tgaatgaaac tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt
     7561 tgtttctagg aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca
     7621 atagcagaaa aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt
     7681 tacgaataca tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt
     7741 ttttcttgaa tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct
     7801 tgagctgata gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga
     7861 aatattggag catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta
     7921 agaatttagc aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta
     7981 gaacttgcgg ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt
     8041 gactatggtc aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg
     8101 agatagccac ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc
     8161 agcgtacttc cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt
     8221 agctgactgt gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt
     8281 gctcaatatt ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc
     8341 tttctccagt tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat
     8401 aaggatgccc ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc
     8461 acatcagtac gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt
     8521 gataccagtt aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt
     8581 ctttagaagc aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct
     8641 tattgtagca gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt
     8701 aaatatgaaa aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca
     8761 atactttata ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa
     8821 tgataaaacg caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa
     8881 aataaccata catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag
     8941 caatactaag tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag
     9001 atagatagtc tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga
     9061 tatgaaacaa aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt
     9121 gaacgtagat agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg
     9181 ctttttttat ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag
     9241 ccccgtggca gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa
     9301 acttcatatc ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg
     9361 gttgatgggc tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga
     9421 aacaaaagca gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga
     9481 tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga
     9541 tgattaccat aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat
     9601 ttacttaggt gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac
     9661 acaacttgca gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag
     9721 tggacacgaa gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc
     9781 agaccgtcta ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa
     9841 aaacgaaatg atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac
     9901 aacttttact tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat
     9961 cgatgcatta tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt
    10021 cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac
    10081 gatctattgg ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg
    10141 gcatggctgg ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta
    10201 tcctgaaatg cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc
    10261 tgttccgttt gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta
    10321 tgatctggca aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata
    10381 ttgtgatgga ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc
    10441 tacgacggct gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa
    10501 tacagcattg gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac
    10561 agaaccatgg gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat
    10621 ccatttagct gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac
    10681 gccagagaag atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt
    10741 acactttggc gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc
    10801 acgtttagat atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca
    10861 aacaaacgaa gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga
    10921 aaaacaaatc aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa
    10981 ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag
    11041 ccaagaccgt caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat
    11101 cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag
    11161 tcaaggaatg atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc
    11221 aaaatccatg ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt
    11281 tgtgtcatac cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta
    11341 cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag
    11401 aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac
    11461 agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt
    11521 ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat ctatatttat
    11581 atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt
    11641 gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg
    11701 atgattgttt tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt
    11761 tttgtcttca aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca
    11821 taccagaaaa tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt
    11881 aacaagatca accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg
    11941 acttcactaa gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc
    12001 atccgccagt tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa
    12061 ataccgatag gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc
    12121 acaggacctt tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt
    12181 gataacgttg caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac
    12241 tgctgttgtt ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga
    12301 taagaacgct taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac
    12361 gaaaaataaa aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa
    12421 aaagaatggt aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa
    12481 gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag
    12541 ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg
    12601 ctagtactca gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga
    12661 tctgtgctgg attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg
    12721 taaaatatat tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa
    12781 gcttgattta ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga
    12841 cgaacttatt acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt
    12901 ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt
    12961 tgattgttaa attaatacca attgctgtga ttgtcatatg gggattattg accccaggac
    13021 aaggaactgt ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat
    13081 tgagcagtgc gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa
    13141 tggcagggga aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga
    13201 gttttgtaac agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg
    13261 atcatcttgc cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa
    13321 tcggtggaaa gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt
    13381 atacactaac tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca
    13441 gtaaacagct gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc
    13501 agttagcagt agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt
    13561 tgatttttgt gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa
    13621 aaaaagcgcc ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta
    13681 ttgcgattct cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag
    13741 cattgatcgg tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa
    13801 aacgctcaga agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct
    13861 agatcttcgt gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact
    13921 tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt
    13981 gattctcata cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag
    14041 cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt
    14101 gaaaagtctt tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat
    14161 ccgacagagg ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact
    14221 ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg
    14281 aaagaagtcc aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta
    14341 ccaatcagta ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa
    14401 gatgttcgag atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa
    14461 cgatttcttg acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca
    14521 ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat
    14581 gccccttatc tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt
    14641 tatacagtag aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa
    14701 acatggacaa atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca
    14761 agagattata gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc
    14821 agatacgata gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca
    14881 tgcccaagag actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat
    14941 aagaaaaatc attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca
    15001 agctaaacca aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt
    15061 agaagcattg aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac
    15121 tagacaagac cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga
    15181 aaaactaggt gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt
    15241 gaagatgttt cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca
    15301 acatgagtga atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac
    15361 atggtttttc atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa
    15421 ggaaaatcgt agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag
    15481 gaatcggtgc attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga
    15541 tcgatgatcg tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta
    15601 ttgtacatca agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg
    15661 aggagcttcc gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga
    15721 tgcattttct tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag
    15781 tagctgatcg tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg
    15841 ttcaatatta tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc
    15901 agccaaatgt agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag
    15961 tgacagacga aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa
    16021 cgctttggaa taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag
    16081 caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac
    16141 aagaattcgc cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt
    16201 gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    16261 ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16321 ttaagaaaga ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa
    16381 acagcttctc atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact
    16441 attatgccga acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg
    16501 atcaaaaaat tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat
    16561 gtgtcttgac agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat
    16621 attaggagag agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat
    16681 tgattgggat gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt
    16741 cctttacttt ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg
    16801 cagtggctgt tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg
    16861 cattgcgagc attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc
    16921 atccgagtat cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt
    16981 tcaattttgt gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt
    17041 actttattgg taatatgcct gaaacatatt attctcaagg atatttttat acagtattca
    17101 tcttgatggg gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta
    17161 tagcaggaac actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa
    17221 atcggaaaga gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca
    17281 aaaaaaaaac tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc
    17341 taaaaagagc tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt
    17401 ttgatgactt gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg
    17461 tcgactattt cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta
    17521 gaaccagttt ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct
    17581 gatttcacta cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct
    17641 ggacgaatac cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt
    17701 tttccttcag gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat
    17761 gtcacattaa agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg
    17821 ggtgtatcat aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata
    17881 acgattcggt cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt
    17941 gtttcaagac gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca
    18001 tctaaatttg ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt
    18061 cccaatgcca cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat
    18121 tctgtcagac ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca
    18181 tatttacgta atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat
    18241 gcgtagtttt ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact
    18301 tttccgccga tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa
    18361 gtagttgact ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt
    18421 gatagattaa aatcagttac agaataattt tctgcattgt catatttttt atatacgttt
    18481 ttcagagcca tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt
    18541 taaagtataa atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt
    18601 ttttttgtca atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat
    18661 acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct
    18721 ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca
    18781 atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca
    18841 accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga
    18901 gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca
    18961 cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg
    19021 ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta
    19081 gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc
    19141 gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga
    19201 taatatgcga tataatacga acgtattgga taatataaac aataatatta gtttttcgta
    19261 tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac
    19321 agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga
    19381 cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa
    19441 ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt
    19501 attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa
    19561 aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc
    19621 agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt
    19681 ctttattgga tttacattga atgcatttat tgaaggagca atctacggat tctttttcta
    19741 tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat
    19801 caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg
    19861 ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc
    19921 catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca
    19981 cgctaaataa aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg
    20041 tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg
    20101 tgcccatttc gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg
    20161 tggacaggcg atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt
    20221 gttggatttt tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca
    20281 tagggggatt actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata
    20341 tctttaaccc ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt
    20401 catacatata agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc
    20461 caaaacgaag attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc
    20521 caaaactttt acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa
    20581 ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg
    20641 cccctgtttt tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt
    20701 cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt
    20761 tgatattttt gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat
    20821 cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc
    20881 cagaaagttc gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca
    20941 caccaacagt gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg
    21001 ataaataagc aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt
    21061 gtgcccgttg tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag
    21121 cttcttcttg ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc
    21181 ccgagatatc cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa
    21241 cgatccaact cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg
    21301 gacttagacg catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt
    21361 gattgaaggc cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga
    21421 cgatatttcc gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa
    21481 atgcatcttt tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga
    21541 tgagccattg actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga
    21601 acagtcgatt gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt
    21661 gtaacgtcat cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa
    21721 tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt
    21781 tttttacttt cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga
    21841 ttgagcaagt aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa
    21901 gatatgatgt gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc
    21961 aagataagtc tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa
    22021 agagtcatct aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg
    22081 ctgtttttgt ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt
    22141 tggaagctga cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt
    22201 ttctgtggcg tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac
    22261 agaagtttgg ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac
    22321 atccattccg tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg
    22381 tgtcaaagtc gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt
    22441 cagctgaaat gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac
    22501 atggtcaaaa gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc
    22561 aagaatcgaa ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg
    22621 tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata
    22681 attgatcaaa gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc
    22741 aactgtcagc aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag
    22801 ggcagagata ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa
    22861 cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt
    22921 ctgactaacg tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac
    22981 ggtttttttg atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa
    23041 cagacctact tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg
    23101 tgctctgatc aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt
    23161 tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt
    23221 attgattttt ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga
    23281 agcatagtat tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca
    23341 taagcccatt tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga
    23401 agccatcatt aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc
    23461 caaaatgatt tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct
    23521 cctattattt atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa
//
LOCUS       DAZUJO010000041        23253 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000041 DAZUJO010000000
VERSION     DAZUJO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.041"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..682
                     /locus_tag="ACG8OL_001673"
     CDS             <1..682
                     /locus_tag="ACG8OL_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4817662.1"
                     /translation="YNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSW
                     VIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSII
                     YFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLF
                     YNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARR
                     FDSDSALF"
     gene            694..1614
                     /locus_tag="ACG8OL_001674"
     CDS             694..1614
                     /locus_tag="ACG8OL_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817663.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1655..3112
                     /locus_tag="ACG8OL_001675"
     CDS             1655..3112
                     /locus_tag="ACG8OL_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4817664.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3457..4071
                     /locus_tag="ACG8OL_001676"
     CDS             3457..4071
                     /locus_tag="ACG8OL_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4817665.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4064..5788
                     /locus_tag="ACG8OL_001677"
     CDS             4064..5788
                     /locus_tag="ACG8OL_001677"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4817666.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5785..7236
                     /locus_tag="ACG8OL_001678"
     CDS             5785..7236
                     /locus_tag="ACG8OL_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4817667.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7708..9861)
                     /locus_tag="ACG8OL_001679"
     CDS             complement(7708..9861)
                     /locus_tag="ACG8OL_001679"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS4817668.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9863..10909)
                     /locus_tag="ACG8OL_001680"
     CDS             complement(9863..10909)
                     /locus_tag="ACG8OL_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4817669.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11059..12348
                     /locus_tag="ACG8OL_001681"
     CDS             11059..12348
                     /locus_tag="ACG8OL_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS4817670.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12361..15063
                     /locus_tag="ACG8OL_001682"
     CDS             12361..15063
                     /locus_tag="ACG8OL_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS4817671.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15048..15905
                     /locus_tag="ACG8OL_001683"
     CDS             15048..15905
                     /locus_tag="ACG8OL_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4817672.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15976..16833)
                     /locus_tag="ACG8OL_001684"
     CDS             complement(15976..16833)
                     /locus_tag="ACG8OL_001684"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4817673.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17044..19215
                     /locus_tag="ACG8OL_001685"
     CDS             17044..19215
                     /locus_tag="ACG8OL_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4817674.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19283..19624
                     /locus_tag="ACG8OL_001686"
     CDS             19283..19624
                     /locus_tag="ACG8OL_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS4817675.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19900..21654)
                     /locus_tag="ACG8OL_001687"
     CDS             complement(19900..21654)
                     /locus_tag="ACG8OL_001687"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817676.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21665..22501)
                     /locus_tag="ACG8OL_001688"
     CDS             complement(21665..22501)
                     /locus_tag="ACG8OL_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS4817677.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22503..23186)
                     /locus_tag="ACG8OL_001689"
     CDS             complement(22503..23186)
                     /locus_tag="ACG8OL_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS4817678.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7642 a         4158 c         4783 g         6670 t
ORIGIN      
        1 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
       61 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
      121 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
      181 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
      241 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
      301 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
      361 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
      421 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
      481 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
      541 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
      601 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
      661 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
      721 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
      781 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
      841 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
      901 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
      961 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     1021 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttgattac gatgttgttc
     1081 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     1141 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     1201 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     1261 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     1321 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact ctacatccaa
     1381 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     1441 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     1501 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     1561 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     1621 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     1681 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     1741 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     1801 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     1861 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     1921 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     1981 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     2041 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     2101 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     2161 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     2221 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     2281 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     2341 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     2401 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     2461 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     2521 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     2581 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     2641 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     2701 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     2761 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     2821 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     2881 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     2941 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     3001 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     3061 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     3121 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     3181 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     3241 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     3301 gttcaatcag ctgaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     3361 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     3421 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     3481 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     3541 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     3601 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     3661 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     3721 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     3781 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     3841 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     3901 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     3961 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     4021 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     4081 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     4141 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     4201 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     4261 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     4321 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     4381 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
     4441 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
     4501 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
     4561 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
     4621 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
     4681 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
     4741 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
     4801 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
     4861 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
     4921 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
     4981 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
     5041 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
     5101 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
     5161 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
     5221 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
     5281 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
     5341 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
     5401 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
     5461 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
     5521 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
     5581 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
     5641 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
     5701 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
     5761 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
     5821 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
     5881 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
     5941 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
     6001 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
     6061 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
     6121 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
     6181 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
     6241 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
     6301 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
     6361 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
     6421 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
     6481 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
     6541 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
     6601 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
     6661 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
     6721 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
     6781 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
     6841 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
     6901 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
     6961 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
     7021 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
     7081 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
     7141 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
     7201 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaagccaatg taatgaaata
     7261 acttataaaa aattgtctag aaatctaatt ttgggctgtt cttattactt gaggcaaggc
     7321 agctttttca tgacctctcg ataaacggag ttcaaaagcc agtttctcgg tattaagcca
     7381 aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttaatac tcgaaactaa
     7441 acagcttttt cacgacctct ccactaaatg gtgttcaatc agctgaccct cggtattagc
     7501 tcaaaaaatg gcaaaatatg aggaactatt ttgccatttg tctctctaat actcaggtca
     7561 gaacgctgat ttcacaacct ctcaataaac ggtgttcaaa agcttgcatc tgcggtaata
     7621 agaccaacca aacgaaaaat atgaggatct atttttgctt ggctgttctt attacttgat
     7681 gcaggacagc tttttcacaa cctcttgtta attatagttt gataaggaaa aaggcaaatc
     7741 ttttgcttga tatttcttag ggtgagggat gatccctaat tggtaagaga tcgtgccgcc
     7801 tttcagcaaa tcttgatggg taaaatataa tttcgtatat tctctgtcca tccgtttgat
     7861 tttatggata aattgttgct gcggccgatt aggcgttgct tcaatcgtca attcctttcc
     7921 atttgacaaa agcaaaacag ccttgtcaat taatggcata ccgataacat attcaccggt
     7981 tccaggcgtg acaggataaa atcccagact attgaagata taccaagcgg ccatacttcc
     8041 attgtcctca tctccagggt aacccattgg agaatcgtcg aaggtctgag tcatcaactg
     8101 tttaagcagt ggctgtgcca tttcaggttg tcctatataa ctgaacaagt aaggatagtg
     8161 aaaacttggc tggtttgaaa tagctaattg tccaaattct atcgcggcca tctcactcat
     8221 ttcgtggatc tcaaaaccat atcctcctgg gtcaaacgtt gggggctgat tgcacagctc
     8281 aatgagtttt tctttatatc tctcactgcc accatatttt ccgatcagtc ctgaaaaatc
     8341 gtgataaaca gcaaagctgc tttgccaagc acttccttct gcataatctt gtccccaacg
     8401 attagctgaa aatggttctc tgaacttgcc atctttatct tttgaacgca taaatcctga
     8461 ctcatagtca aagatatttt gatagttcaa tgattgctgc tgatatttct tcgtcatttc
     8521 ctcttcttct agtaccttgg caacttggct aatacaaaaa tcgctataag catagtctaa
     8581 tgtgtgattg actgattcgt ggtagtgatt aggaacatag ccatatgtca agtaatcttc
     8641 tgttccttgg cgtccataat ttggatgttt gctttgaacc gtagcacctt tttccattgc
     8701 cgttagaaat tcaggcatca agtctcgtcg gatatctttt acagcagcat ctgcaatgac
     8761 cgcatcgatc aatgtcccag gcataagtcc acgttcatca ggagacagcc attttggcaa
     8821 atagccactt tctctatagc tgttcaaaaa accttccagc atttcttcgt atttttctac
     8881 agcaatcaaa gaataaagag gataaaccgt tttatacgta tcccaaaacc cattatttgt
     8941 atagagaaca ccaggtttta tagatcgact agtcgtatca tagtggattt tttgcccact
     9001 aggatcaatt tcataaaatg tttgtggaaa taaaaacacc cgatataaat tatgataaaa
     9061 cgtccgcaat tgttgttgat catggtgtgt gagctgtata cgatcaaaat aggactgcca
     9121 ttgttcttga ctgtgagcaa ggtaagtctc tttgctttgg ttttgttcta gttccaggtt
     9181 ataagctgct tgttccgagc tgataaaaga tgtcccaaat cggatagttt gtttttttgt
     9241 ctgttcaaaa tacaacggga tcgagtcgct atttttgttg gaaacagcta actcatcaat
     9301 tgttaaagga tattcaaatc tcaatataaa ataaaaagaa aaatcaggat cttctgaacc
     9361 agcaaaatgt gtgatttttc cttccactgt gtaaggatct ataacaaaga gctgatggcg
     9421 tcccggaaag gataaaagta aaccgctgtc atttttgctg taatcaatag ataagattcc
     9481 gccatacatg ctaggtatca atgtagaaca gatgccataa cgtaattgac agatcgacag
     9541 atgagtcggg caaaatatac tttcttctgg gcggtaagaa ctttgagcat ggaaaagggt
     9601 gttctcctgt agttttccat tgatcggcgt aaacagcata tgactgaaat cacccatcca
     9661 aggacttggt tgatgggtaa gacggtagcc ttgaaaaacg cggtcatcag gatgaaacca
     9721 ccagctgccg ttttgatccg tagtttgcgg tgcaaaataa ttcatcccaa aaggaacgcc
     9781 agtataaggc tggcagttcc cattagagaa atttggctga ttcgctgttg catgtctagt
     9841 atctatttga ccgatattca ttttatttct cctttgttga ttgtcttttg atgaatgaaa
     9901 ctggaaattt actatcggat ggtcgatctt ttgtctctaa ccaagtgacc aaagcatctc
     9961 ccgcaagctt acctaactgt tcaaaatctt gtgcgatggt tgtcagtggc gggtcaacaa
    10021 gagctgctgc ttggatatca tcaaatccga taattgaaaa atcttcaggg atatgataac
    10081 catgctgtct caagagattc atggtctgga tagcgacaac atcgttttca caaacaaaag
    10141 cagttacttc atacttagtg atataagaaa gcaattcatc taatgtcgcg cttttgtcat
    10201 ccagtgaagt gtggaaagtc aaaccagctt tgtccaatgc ttcaaggtaa ccaaggtaac
    10261 gctgtcgaac ggattgtgga tgagtctgcg taccaaacaa ataagcgatt tttgaatgtc
    10321 cctgttcgat caaataagag gcagcaagcg caccgccttg gaaattatcg gaaagaacgg
    10381 taggaaaagg taactcatag atttttttat ctaatatgac gacagggaat tctttcaatg
    10441 aaagctcaaa aagtaagtca agatgttgtt ctgtatcaag agcataataa atcaatccgt
    10501 caaattcatt gattagctct tttgctgttt tattttgtag aaagtcaagc gtcgtcatca
    10561 tgacatcaat ctgtttatct tgcatcacag ggttcaatcc cttgctaaaa tcaccaacag
    10621 acaaatcagc gacatatgga agaagaaaca gaatccgtcg tgtttttgta ggagaagcaa
    10681 gaatctgttc cggagaagcg gatttaacga aactgccttt cccgcgaacg cgataaataa
    10741 gtcctaattg ctctaattca gtcaatgcac gtttggaagt gattctgctt actttatagg
    10801 tacgtgaaag ttctttttca gttggtagtt ggctgtctac aggtaattca ccagttaaaa
    10861 tagccgtttg caaatcatta aatattgtct ggtacaatgg ttgagtcata taaacacctc
    10921 ttaatgatat atcatattca aataatacca taatggatga aaaagtaaat aaaaagatat
    10981 tttttgaaag cgtagacata attggctgta tgaagtataa tctttaaaga tatatcaaat
    11041 agaaagaagg ttttattagt ggtttataaa gatataccaa aatcggtgca gaaatttatg
    11101 gaagatatca cacaaaaatg tggggatatc catgcggact gggcaaagaa ttttacagca
    11161 gcatttgcca atactctgtt gacaacagtc aaaaagcaaa atgacggaac caccttttta
    11221 ttgacgggag acattccagc gatgtggtta agagactcta ctgcacaagt tcgtccttat
    11281 ttagtgattg caaaagaaga tgaggaatta gctgacatga tcagcgggtt agtcaaaaag
    11341 caattttttt atatcaatat cgatccatat gcgaatgcct tcaacgaaga agctaacggt
    11401 gctggccacc aaacagatca tacaaaaatg aacgattgga tctgggaaag aaaatatgaa
    11461 attgattctt tatgctaccc aatacagttg gcctatcttc tttacaaaaa tacaggaaaa
    11521 actgatcaat tcaatcaatc ttttattgaa ggtatcaaaa aaatcttgga cgtgttcaaa
    11581 acagagcaag atcatgaaca ctctccttat acatttgtac gagacacgac aagaaaagaa
    11641 gatacgctga tcaatgacgg gcgaggtgca aaaacagctt ttacaggaat gacatggtct
    11701 ggtttccgtc ctagcgatga tgcttgccgt tatggatatc tggtaccatc caatatgttt
    11761 gcggtggtcg tgttaggtta tgtggaagaa atttttcaaa cgatactcga tgataaagaa
    11821 attgtacagc aaacacagaa attgaagaaa gagatccaag aggggattca gaatcacgga
    11881 cgaacggtta atcaaaaagg gcaaacgatt tatgcatatg aagtagatgg aatgggaaac
    11941 gccacagtca tggatgacag taatgtgccc aatctgatcg ccgcacctta cttgggatat
    12001 tgttctgaac aagaggaaga ttatctgcaa acaagaagaa cattgttgag taaagaaaat
    12061 ccatatttct atgaaggaaa gtacgcaaaa ggaattggat cctcccatac gccagaaaaa
    12121 tacgtttggc cgattgcatt agctatggaa ggaatgacaa cgtcagacaa atctgaaaaa
    12181 gagcggatat tgaatttact tgtagcaact gacgcaggaa cacaccttat gcacgaagga
    12241 ttcgatgtga acaatcctga aaattatacg agagaatggt tttcttgggc aaatatgatg
    12301 ttttgcgaat tagtcatgga ttactttgat attagagtag aaaagtagga ggtatcgtaa
    12361 atgaaaaaga aagtatatat catttctcat agccactggg atcgtgaatg gtacatggct
    12421 tacgaacagc atcacatgcg actgattgaa ttgatagacg atctactaga gctatttgaa
    12481 gttgatccgt ccttcaacag ttttcatttg gatgggcaaa cgattatttt agatgactat
    12541 cttcaagtaa ggccagaaaa aagacaggca atccaacaag caatcaatga aggaaaattg
    12601 agaattggac cattttatat cttgcaagat gattttctta tcagctcgga atcgaacgtg
    12661 agaaatatgc tgattggcat ggaagaaagc cgtaaatggg gaacccctgt catgttaggc
    12721 tattttcccg atacatttgg aaatatggga cagacgcctc aattaatgaa acaagctggt
    12781 atttcagcag cagcgtttgg ccgtggtgta aaaccaattg gctttgataa tcaagttttg
    12841 gaagcagaaa actattcttc ccaatactca gagatgtggt ggaaaggccc tgatcaaaca
    12901 gcaatctttg gtctgctatt tgccaattgg tatagcaacg ggaatgagat tccagtagaa
    12961 aaagaagcag ctcttgcctt ttggaagcaa aaattagcgg atgcagaaca atatgcctct
    13021 acgaaccact tattgatgat gaatggtgtt gatcatcaac cggtacaaaa agatatatca
    13081 aaagcaatcc atctcgcaaa tgaactgttt ccagattacg agtttattca ttctaatttt
    13141 actgactatc tggaagcagt tcaaaaagat gtgccagaag acttaggctc agtagaagga
    13201 gaattgacaa gtcaagaaac ggatggctgg tatactttag ccaatacagc atctgctcgg
    13261 gtttacttaa aacaatggaa taccaaggtc cagcgacagc tagaaaatat tacagaacca
    13321 cttgcgacga tggcttatga agttagtgga aactatcccc atgatcaatt agattatgct
    13381 tggaagacat taatgcaaaa tcatcctcac gacagtattt gcggttgttc agtcgattct
    13441 gtccatcggg aaatgatacc tcgttttgaa aaagcagatg aagttggaaa gtatcttgca
    13501 caagattcct tagaacagct gaccgcagca atcgatacga ccggattccc aaaagacagc
    13561 tttccatttg tcattgtaaa tactgcagga atggataaaa ctggtgaagc agaaataacc
    13621 atcgaattag aaagaaaaag attcgctgaa ggaatacctg aacaactata tcaagagtta
    13681 gaaaatttgc ctaaacgtaa gtatcatgta gaaacaaaaa gtggggccac tataccagct
    13741 attttatctg aagaaactgt ccaatttggt tatgatttgc cgaaagaccg tttccgtgtg
    13801 ccatatatgg caagaatgat caaagtgact ttaccattag aaaacatgcc agcattttct
    13861 tgggaaacat tcgcgcttgt agaaggggaa gcgaaagcag aagaaaagga aacaatgatt
    13921 catcagagtg gacgaatcat cgaaaacggc cctctacatt tgacgattga aaaaaatggt
    13981 accattacaa tggaagatcg caaaaacaaa cgaaagttga ataatctgca tatttttgaa
    14041 gatatcggcg atataggaaa tgagtatatc tttaaacaac ctttctgtga tcagccaatc
    14101 ctatccagta acaaagaaaa ctctgaagta aaagtattag tagatacacc ggaaatagca
    14161 aaaataagta ttctacagga aatggaaatc cctgtttctg cagacgagcg tttagaaaaa
    14221 gaacagcaga tggttgtgga atttcgttat cgaaaagcag aacgatccaa agaaaaacga
    14281 atccttcaaa taaaaacaat catgacaatc agaaaagata gtaaaaaaat cgactttgaa
    14341 acgactctcg acaatcagat gaaagatcat cgcttacgtg tcttgtttcc tacaaagtta
    14401 catgtggaac atcacgaagc agatagtatt tttgaagtgg tgaagcgccc aaaccatgtg
    14461 tctaaaagtt gggaaaaccc cacgaatccg caacatcagc aagcatttgt caatattcat
    14521 gatgaagagt acggtgtaac agtcggaaac tttggtttga atgaatatga agtaacagaa
    14581 gacggacaaa tcgcagtgac gctacttcgc agtgtgggag aactaggcga ttggggatat
    14641 tttccaacac cagaagcaca gtgtctaggt gagcatcggt tcaattacag cattgaacta
    14701 catggaccag aagaaaaatt ctctacttat ttgcatgcat acgcagcaca gattcctttt
    14761 tccactcaac aaatcaaaca tcatgaagga acattgattt caaaacaaca atatttaacg
    14821 ataaaaagtg aaacgtttgc aattacagct ttgaaacgca gcaagtttag tgataaagta
    14881 gtagtaagag gtttcaatat gtctagtcat ttagaaaaat tggaaataac taaagacaat
    14941 ggaaaaactg tcatattgaa tctgcttgaa gaaccaacga agcaagcggt tgtcccaata
    15001 atccaacctt atgagattcg gacaatcgga ttcgaggagg aaaacgaatg tatggcggaa
    15061 tagaagcagg cggaacaaaa tttgtgtgtg cggtttcaga tggtggagaa attttagaaa
    15121 aattaagtat ccctaccaca acaccagaag aaacattgga tcaggtattt gaattttttg
    15181 atcgttatga attgaaggga atcgggattg gctcattcgg cccgattggt atcgacaaaa
    15241 caaaagataa ttacggatac gttttggcaa ctcccaaaaa agggtgggca caattcgatt
    15301 ttcttggcac aatcaaggaa cgctacaata ttcctgctgc ttggacaacg gatgtcaatg
    15361 ctgctgcata tggggaattg aagcaaggag cagcacaagg aaaaaatagt tgtatttacc
    15421 ttaccgtggg aacaggaatc ggtgcaggtg tcgtgatcaa tgaagaaatt ttttcaggga
    15481 tcgcacatcc tgaaatggga catatttggg tgaaacgcca tccagatgac cattatgaag
    15541 gtacttgtcc ttatcataaa gattgtttag aaggattagc agcaggaccg tctattgagg
    15601 cacgcacagg gatcaaaggg caagatctgc cgcaagatca tcctgtttgg gctattcagg
    15661 cttattatat tgctcaagca ctgatcaact atacgttgac gttagcacct gaaaagatta
    15721 ttttaggcgg tggggtaatg aatcaggatc atctccttca aaaaatccgt cagcaatttg
    15781 ttgaattgat gggtggttat atggaaacac ctcaagtaag tgaatatatc gttcgttggg
    15841 gactgccgaa tgaaagtggg attatcggta gtttgctgtt agcagaacac gaacatcaaa
    15901 agtaagcaga caaattacac aaaaagaaca gagtcttatc attatctgct gataagactc
    15961 tgttcttttt tatttctatt cccataatcc aggtaaagct ttaccgacta ttttttcttg
    16021 agtaaatgga tggatcatgt gcagttctaa tgcatggagc atcagtcgtt cagctgttct
    16081 attttggtag agagggtctc caacgatcgg atgtccctta tgagctaaat gaactctgat
    16141 ctggtgtgta cgtccggttt ccaaaacaca atagacttgt gtttgttcag aaagtacttt
    16201 gttgacagtg acatgtgtca aggcgctctg accattttta tcatctacga ccctttttct
    16261 ccgatcatgg cgatctcgtc caattttatt ggtgatggtc atatctttgg caatttctcc
    16321 ccatacgatg gcttgatatc tccgatagat cattttttgt tccaacattc ttccaagtat
    16381 cggcaagaca aatggatttt ttgcaaataa aacggcaccg cttgtttctt tgtccaagcg
    16441 gtggacaacg taaggtcgtt gctgtttagt gtcgagataa gcagctaaat gattgagtaa
    16501 ggtactgttt tctgtcggtt catttggatg agtcttcatt cctgcaggct tatccactat
    16561 aatcaaatgc tcatcttcat ataaaacacg gatgtgcttc gcatctccta attgaacttc
    16621 ttgataatta taatctgttt cttcgaataa caatgtgatc tggtcattgc cctttacttc
    16681 atgatggaac aaagcaggtt gttgattgat ccaaacgttt ttacgtatac gcaaaaagtg
    16741 tcgaaccttt ctaggaacta accattcctt ttcaagcaag tcgcgaattg ttgtttcagg
    16801 atggttcata ggtaaagtga tcgtgatttt cacaagtgta ctcctttcag tgcaattgca
    16861 gactaattgt accattttta aaacatattt aagaaaaaac ttacattttt ctgttcattc
    16921 ttatgttact ctacttgcac taacgtaaaa aaacatattg aactacaact taagcaggaa
    16981 gaaaacactc tttcactatg gtaaaataag acgattgaat caaatagaca ggagaagaag
    17041 caaatggatt ttcaaagcat tttagaaaag ataaaagctg ctttgatcag tttttggcgt
    17101 tggatcagac catatctcgg acaattccat cgatggcgaa agcgcatctg gaaaaaatat
    17161 catgtgaaca aattgatttt gctgattggt ttgatctgtg tcttagtgac aagtatctac
    17221 ctctttattt tagcaaaaca agcaaatgtc gaaacgttaa aatcaggatt aagccaatcg
    17281 acagtcattt atgataagaa aaacgaagaa gctggcacac tgtacgccca aaaaggaaca
    17341 tatgtagaat taaacgcgat ttctccctat atccaagatg cagtaatctc gacagaagat
    17401 aggaattttt acgagcatca cgggtttgat atcaaaggga ttgcccgtgc agcagtccga
    17461 atggtcttgt ctggcggaac atcagggggc ggaggaagta cgatcactca gcagcttgcc
    17521 aaaaatgcgt atctgacatt agaccaaacg tttgatcgaa aagcgaagga attatttttg
    17581 gcaatcgaga tcgaaaagaa gtacagcaaa gaagaaatct tgaccatgta tcttaacaat
    17641 gcctattttg gcaacggggt atggggtgtc caagacgctt ccagaaagta ttttggagta
    17701 gatgcaacga atgttacgct gtctgaggct gctactttgg caggaatgct gaaaggtcct
    17761 ggtatttata atccgatcga tcacccaaaa aatgcgaatg atcggaaaaa cacagtacta
    17821 agtgtaatgg aagaaaacgg aaaaatcaca aaagaacaag aacaagcaga aagccagaca
    17881 gatatcagcg cgtatcttaa tgacacatac gataattcag attctggtta tcgttatcct
    17941 tattattttg atgcagtgat cgatgaagca gttaacgagt atggtcttga tgaagaagat
    18001 gtcatgaata aaggctataa aatctataca gctttagatc aggattatca aaagcaatta
    18061 gaagctactt atcaaaacag tgcattattc ccagcaaatg cttcagatgg tgccatggtc
    18121 caatctgctt cagtagcttt agatccgaat acaggtggtg tacaagcact agtaggacga
    18181 agaggagatc atgtctttag aggattcagc tttgctacac agatgcgtcg ttcaccagga
    18241 tcaacgatca aaccattaag cgtatataca ccagcactgg aagctggata caagccttcc
    18301 agtatcttga aagacgaacc gcaaagttat tacgatgcgc ataattttga cgggacatac
    18361 caaggagaag taccgatgta tcaagctgtt gcacaaagtt tgaatttgcc tgctgtatgg
    18421 cttttgcatg agatcggttt acaaaaagga tatgataaag cagaagaatt cggcctgcct
    18481 ttagcaaaat ctgataagta ttatggctta gcccttggtg gactggaaaa aggagtatct
    18541 ccattgatca tggcgggtgc ttacagtgca tttgccaatg atggtcagat gtatacgccg
    18601 catcttatca cgaaaatcgt tgattcgact ggagcggtga tcgtcgataa cacaaacaaa
    18661 aaaccaaaac aggtcatatc aaaggaaaca gccgatgaaa tgaccagcat gcttcttggt
    18721 acattttcaa atggaacagc ggctgctgcg aatcctgcag gttatacgat agcagggaaa
    18781 acaggaacca cagaaactaa tttcgatgcc acaaaagcaa atgaccaatg gatgatcggt
    18841 tacacgccag acgtagtgat ttcgacatgg atgggttacg aagaatccag caagctgcac
    18901 tatttggaag ggacaagtgg aaatgttgtc gggaaagtct tcaaatcagc ggcagaaggc
    18961 atccttccgt atacttcaaa aacaggattt agtgtagcag atgcctatgc cacaggagga
    19021 caagtggtag ctgcagatca agttggaaac acaggaaatt ctaatggaga gtctggaaat
    19081 tggcaagata acttgaatca atatggtgaa caagcaaaag aagggctgaa aaacttcggc
    19141 gatatggtga aagaaggcgt tcagggaatt ggtgaagcgg ctaaagacct ctggcggaaa
    19201 tatcaaggag agtagcccct ttgaaaacga ataaccccat gttacaatag agagtaacta
    19261 ctaaaagaaa agagggcata acatgtcaaa catctacgat agcgcgaatc aaatcgaacg
    19321 tgagatccgt gaattaccag aattcaaagc gttgaaagaa gcgtacgaga aagtacaagc
    19381 aaacgaagaa gcacacaaac tgttcaaaga cttccaagcg atgcaaatcg aattacaaga
    19441 aaaacaaatg agtggacaag agttcagcga cgaagatgca atgaaagcac aagaaatggc
    19501 tgtgaaaatc caatcagaag aagtaatcaa tgacttgatg caaaaagagc aaggcttcag
    19561 tacaatcatc aatgacttga accgtatcat catgacacca gttcgcgacc tttacagcga
    19621 ataaaactat tttatgaaac aacagagggt gtgaaagaga cgttcggcct tgagcgatga
    19681 caaaattgct tcttattgcc gataggctgt ctcttgaaca ccatttacca agaggtcgtg
    19741 agcctaccgt ttaactctga gttacaagga acaatttcaa aaaacagctt ctcatgtttg
    19801 atgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcagaaatag
    19861 gagctacgac aaaagtcttg tcatagctcc tattttttgt cattcatttt tcaagcggta
    19921 gaaatagcct ttttgagcta acagtttttc gtaagtacct atttcaacga tttttccttc
    19981 atcgatcaca acgattttat ctgcctcttt gatcgttgaa agccgatgag ccacaataaa
    20041 agtagtgggt gtttctgtca ggtaattcag ggcttgctgg atttttttct ctgactggtt
    20101 atctaaagct gaggttgctt catctagcaa aatgattttt ggctgacgta tcaacgctcg
    20161 tgcaatggag atacattgct tttggccacc agacagcttg ttgccatgtt cgcctactaa
    20221 ggtatccaga ccatcaggaa gctggtcaat cagtgaagaa agttgcgcag cttcgatgac
    20281 ttcatctaaa cgttcttttg atacgttttt caagccataa gtgatgttct ctttgatcga
    20341 agcggagaac aacaaagtgg tttgaggaac aacggctaaa taatttcgca cacttcgcat
    20401 atccagttca tccaaaggct gatcatctag aaaaattttt cctgaagtcg gctggataaa
    20461 tccaatcaat aagttaagga gtgtagattt tccagagcca gatggcccaa caaaagcaat
    20521 tgtttctcca gctttcacct ctagagaaaa atgatcgata atcggtttga gtgagtccgg
    20581 ataaagaaaa gaaacatcct caaaacgaaa atttccatcc aaatgttgga tttttttctt
    20641 accgtgataa gtttctacat cgccagcaat caacagttcg ccaatagaag tgatagattc
    20701 catgcctttt gaaatcgtag gaatcagcgt gatcattcca gaaacggaat tgacgatcgt
    20761 cgtgaaatac gtctgataca tcacgacatc gccgggttgg atgcgccctt gtaaagctaa
    20821 tatcccagtg aaactcaaac aaatcagctg aaatatctga aaactagccc agctgactga
    20881 tccgaaaaca gattgcagga tatctaagtg gaatcctttt tcatagattt gagtaaaatg
    20941 cttatttaag cgagttgttt cgtgtttttc taacccatga gctcgagcaa tcggtgctaa
    21001 ttcgatcact tcgatcactt ttgcagaagt ctcttccatt tcttgcctga agtctgagtt
    21061 ggactgtctg atccttttac ggaaaaagag agtaacgaat gcggcgagag gaacagtcaa
    21121 taagaaaaac atgaaaacaa caggactttt ataaagcgtg atacctaaag ctacgaataa
    21181 attgacagcg atatttaatc cgctgacaaa tacttgagag gacaacgttt ggatagcttc
    21241 tacatcacgg atgatctttg actggattcg tccagattgc aattcttttt gataaggaat
    21301 agatagttct tggattttct tgataagggt cgctcttagt ccagcttcta cttgacggat
    21361 cgaagtgcta tggtatttta catgaaaata attcgtcaat atattttgac caatcagaca
    21421 agccatcaaa aaagcgttga aataaatggc ctgcaactga gaagaatctt tcgatgaagc
    21481 tgcattgatt acattggcaa tagcgatcgg cattaaccaa gatggaagat gtttgataat
    21541 aaaaaagagt cctgacatgg caaaacgcca ataatttccc tgataaagat gcagcaatgt
    21601 ctgaaaagca tttgcatgat atttttgata acttttctgt attcgttcct ccataatctt
    21661 ctccttaaca atcttggtaa ataaactgca agtgttctat tgcttcagga acataaatct
    21721 caggagtagc ttcaagagtg gcgtacatta gaggacgctc atatttgata taatgcagta
    21781 ccgggcggaa atctaaacag cccttgccaa caggtaccat gcggatcttt ccattttcca
    21841 tgatatagtc tttgagatga agcgctgtaa tataaccatg taaattgtcc aaggcacctc
    21901 ggatcacttt ttcctgatca gcatgatttt ctttatgcat caaattcgca caatctaaaa
    21961 tgatttttaa atttggtgaa gctacttcat cgattaaacg tttagctaac tgatagttat
    22021 agattggatg attgatccct gcttcaacag caacagttac gccaaaattt tctgcatgag
    22081 cgactaattc aatgatagaa tttctggcga tctgataagc ttcttcagta aaattttctt
    22141 ttgtgtaacc gacagagaca ctaccggttt ccgtagcaat catcttggct tgataggcag
    22201 agcacagagc aatttgctgt ttgaaagttg ccaatgcttg tgcacgaact tcttgatttt
    22261 tgctggatag gttgatatag catcctaaaa tggaaagttc gatattgttt ttttggaagt
    22321 attcaccaaa ataggcagca ctcccaagtg tcatgtcagt taatttaagt tgttcaggaa
    22381 atgctttttg tggtgcaaat tggatatggg acaagtgaag ctgtgatact ttttgaacga
    22441 tttcttcacg atttcgaaca tctaaatcgt gggcacggat gcctaaattt aatggaaaca
    22501 tctcaaactc ctttttgtaa ataattagca agaggcagac gggattcttt tatcaattca
    22561 acaattattc gggcaacttt atcagcgcct tctggactaa agtgggtatt gtcttttagt
    22621 ccttctgggt aatttttaca agtatcaggc ataagatgca ggtagaattt ttttgtctct
    22681 tcttttgaga aggtctggaa caattcttgt gatcttgaga acacatctaa aagaggaata
    22741 tggtgtttct cagcaaatgc acgcatcgct tttggatagt ctccaagtat atctggattc
    22801 aatgttccgt tttctaagta atctcgtcgc gtcacagatg tcagtaagac catttggacg
    22861 ttcgcttttt ttgcgcattc aaaaaatatc tgcagatttt ttaaatagct accgaaggga
    22921 agcgtacctc tttcttcttg gattttctgg tcgttatgtc caaattgtac aaataagaaa
    22981 tcattcggct gaaactgtga agctagacgg gctaaccttc cttcctggat aaatgatttg
    23041 gtgctgcgtc cattctttgc ttggttattg atctggatct cgggtgaaaa ataagcagag
    23101 attttttctc cccaacctgt ctcgggacgc ttgttttcat ctttttctgc cgcagtagaa
    23161 tcaccggcaa tatggatagt aggcatgtta tctcttcttt cataaaggtt tataggcaat
    23221 gaagcagaac agcaagagaa gaagacggag caa
//
LOCUS       DAZUJO010000042        22634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000042 DAZUJO010000000
VERSION     DAZUJO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.043"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(228..1115)
                     /locus_tag="ACG8OL_001690"
     CDS             complement(228..1115)
                     /locus_tag="ACG8OL_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817679.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="ACG8OL_001691"
     CDS             complement(1112..1981)
                     /locus_tag="ACG8OL_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817680.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="ACG8OL_001692"
     CDS             complement(2118..3404)
                     /locus_tag="ACG8OL_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4817681.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="ACG8OL_001693"
     CDS             3677..4255
                     /locus_tag="ACG8OL_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4817682.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="ACG8OL_001694"
     CDS             4255..5184
                     /locus_tag="ACG8OL_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817683.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="ACG8OL_001695"
     CDS             5181..6917
                     /locus_tag="ACG8OL_001695"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4817684.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACG8OL_001696"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="ACG8OL_001696"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS4817685.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="ACG8OL_001697"
     CDS             complement(7813..8322)
                     /locus_tag="ACG8OL_001697"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS4817686.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACG8OL_001698"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="ACG8OL_001698"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS4817687.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="ACG8OL_001699"
     CDS             complement(9623..10714)
                     /locus_tag="ACG8OL_001699"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS4817688.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACG8OL_001700"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="ACG8OL_001700"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS4817689.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACG8OL_001701"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="ACG8OL_001701"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS4817690.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACG8OL_001702"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="ACG8OL_001702"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS4817691.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14677..15651)
                     /locus_tag="ACG8OL_001703"
     CDS             complement(14677..15651)
                     /locus_tag="ACG8OL_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS4817692.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15632..17515)
                     /locus_tag="ACG8OL_001704"
     CDS             complement(15632..17515)
                     /locus_tag="ACG8OL_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS4817693.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17533..19758)
                     /locus_tag="ACG8OL_001705"
     CDS             complement(17533..19758)
                     /locus_tag="ACG8OL_001705"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS4817694.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19873..20907
                     /locus_tag="ACG8OL_001706"
     CDS             19873..20907
                     /locus_tag="ACG8OL_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4817695.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20974..21987)
                     /locus_tag="ACG8OL_001707"
     CDS             complement(20974..21987)
                     /locus_tag="ACG8OL_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4817696.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22065..22253)
                     /locus_tag="ACG8OL_001708"
     CDS             complement(22065..22253)
                     /locus_tag="ACG8OL_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS4817697.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6446 a         4863 c         4096 g         7229 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt acctagtata
    14461 gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt tctgatagtt
    14521 aactttgttt ttcagaaaaa taacaattgg ttttattgaa gaaaatagtc atgaacaaaa
    14581 gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca gctttgtctt
    14641 gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa agccgatttc
    14701 atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc agctattgat
    14761 acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt ctggcggtag
    14821 ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag cggaaaaagc
    14881 aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt tatccgtatt
    14941 atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca tgccgcattc
    15001 ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac cttcgacacc
    15061 accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa aaaattttgc
    15121 tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc ctttttgtcc
    15181 ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg aaattgtcac
    15241 ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg tttcagcgta
    15301 ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct ttttattgtc
    15361 caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc acataccgga
    15421 tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa aaactggaag
    15481 tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc cgcctcttgt
    15541 ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt cgccgaagac
    15601 gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca taataatatc
    15661 ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca ttccctgaga
    15721 attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact tctctgcctg
    15781 tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag cctccttggt
    15841 acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac gaattcccca
    15901 tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca aggtagtcaa
    15961 tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa tcttcgaatg
    16021 ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact tttttgaagt
    16081 aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga gaccaaaaaa
    16141 gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca agaacagccg
    16201 cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg cccatttttt
    16261 gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct gcttggtcgc
    16321 ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg ttgatctttg
    16381 cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg cgtccttttt
    16441 tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca ttgaagtaga
    16501 tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata tactccgtta
    16561 ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc tgaaaatgcc
    16621 cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct tttaacggat
    16681 attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc agctgactgt
    16741 ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga catttgtcat
    16801 ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca actaagaaga
    16861 catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat gcagctgtac
    16921 ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt gttaaatcaa
    16981 attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc aaccaaataa
    17041 acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt agaccttctt
    17101 ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct gaaaagatcc
    17161 cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca tgtccctccc
    17221 acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca atatgactag
    17281 gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat gtttttggtc
    17341 gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac tgtttaaggg
    17401 aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta tgtgcatttg
    17461 attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct ttcatacttt
    17521 tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat caccttgaaa
    17581 atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc gcttcgaggt
    17641 ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat ggaacgctgg
    17701 ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt taggagacac
    17761 aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat aatggccata
    17821 ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt gctttttttc
    17881 ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca tagctgtttc
    17941 tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag ctgatacatg
    18001 agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt tcatacgctc
    18061 cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa acccggcatc
    18121 aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat cagttgtcaa
    18181 atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc cttgtctctg
    18241 tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc atttgatgta
    18301 gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct gccgaacatc
    18361 taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc tacctggctc
    18421 ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga tcatttcagg
    18481 ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta tttcacgtaa
    18541 tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat tgtccgcatc
    18601 tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct cctttgcttc
    18661 atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt cccagttatt
    18721 gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt cacgtagcaa
    18781 ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa ggatcatttc
    18841 aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc tgttgatccc
    18901 accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat ggttgccgga
    18961 ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat gatgaactaa
    19021 agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa atacttttgt
    19081 tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc ctggcaaagc
    19141 aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag ccgctttttc
    19201 tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga tcactgctct
    19261 atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat ccttaagtga
    19321 aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca agccttcgat
    19381 ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta agtctgatcc
    19441 attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat gagaagagta
    19501 ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa catttcctga
    19561 aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga ctggtttgcc
    19621 aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag aaagaaatgt
    19681 atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt tatctgtttt
    19741 tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta ctttttttta
    19801 taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta tttttttgag
    19861 gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt ctctcctgct
    19921 acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac tactcggcaa
    19981 cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa atatatcaaa
    20041 aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga agaacaggat
    20101 atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct gcatttaggg
    20161 atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct agatcagtta
    20221 ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt gttaaaacaa
    20281 aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga tatggtgtat
    20341 gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa tggtcataaa
    20401 agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg gaagcagatt
    20461 tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca tcacttagaa
    20521 aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt acttgcgact
    20581 aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag cgatccaatg
    20641 gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga agagattgtt
    20701 gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt gaccagtgtt
    20761 caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga gcgaattgac
    20821 ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt tagggaaagt
    20881 tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct tcgtttcttc
    20941 atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca caaaatccaa
    21001 tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt attcatgggt
    21061 aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta attctgtcaa
    21121 ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt cagttatata
    21181 cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca attgctcact
    21241 cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga aaaacttcat
    21301 aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc cgcaataaga
    21361 aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt gtttagcata
    21421 cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg cactatctcc
    21481 accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag ctaataaatc
    21541 tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag gcgctaattc
    21601 ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg caaattcttt
    21661 gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata gaacaggaac
    21721 aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat acttggactc
    21781 atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg tttttttatc
    21841 agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag gtctggggga
    21901 aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga agaaccatcc
    21961 gataaattta agaattttct ttttcataag gctccctcag tagttagtat taagcattct
    22021 gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg attttcccca
    22081 aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc aaacaaaaca
    22141 ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg atccaaaggc
    22201 agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc catttcgcca
    22261 tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt tctctaaaag
    22321 aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga cattttcttg
    22381 agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa tcgaataata
    22441 atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga aaataaaaat
    22501 ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca gaatgacgtt
    22561 cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa atcgccaaaa
    22621 taatgtagac taac
//
LOCUS       DAZUJO010000043        21992 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000043 DAZUJO010000000
VERSION     DAZUJO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.044"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1166
                     /locus_tag="ACG8OL_001709"
     CDS             201..1166
                     /locus_tag="ACG8OL_001709"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4817698.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1265..2437)
                     /locus_tag="ACG8OL_001710"
     CDS             complement(1265..2437)
                     /locus_tag="ACG8OL_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817699.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2424..3569)
                     /locus_tag="ACG8OL_001711"
     CDS             complement(2424..3569)
                     /locus_tag="ACG8OL_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817700.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3583..4507)
                     /locus_tag="ACG8OL_001712"
                     /pseudo
     CDS             complement(3583..4507)
                     /locus_tag="ACG8OL_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4624..5703
                     /locus_tag="ACG8OL_001713"
     CDS             4624..5703
                     /locus_tag="ACG8OL_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS4817701.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5700..6328
                     /locus_tag="ACG8OL_001714"
                     /pseudo
     CDS             5700..6328
                     /locus_tag="ACG8OL_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6438..6665)
                     /locus_tag="ACG8OL_001715"
     CDS             complement(6438..6665)
                     /locus_tag="ACG8OL_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4817702.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6768..9356)
                     /locus_tag="ACG8OL_001716"
     CDS             complement(6768..9356)
                     /locus_tag="ACG8OL_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS4817703.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9376..10035)
                     /locus_tag="ACG8OL_001717"
     CDS             complement(9376..10035)
                     /locus_tag="ACG8OL_001717"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4817704.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(10084..10587)
                     /gene="trmL"
                     /locus_tag="ACG8OL_001718"
     CDS             complement(10084..10587)
                     /gene="trmL"
                     /locus_tag="ACG8OL_001718"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS4817705.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10808..11674)
                     /locus_tag="ACG8OL_001719"
     CDS             complement(10808..11674)
                     /locus_tag="ACG8OL_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4817706.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11783..12424
                     /locus_tag="ACG8OL_001720"
     CDS             11783..12424
                     /locus_tag="ACG8OL_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS4817707.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12486..13847)
                     /gene="mgtE"
                     /locus_tag="ACG8OL_001721"
     CDS             complement(12486..13847)
                     /gene="mgtE"
                     /locus_tag="ACG8OL_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS4817708.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13865..14764)
                     /locus_tag="ACG8OL_001722"
     CDS             complement(13865..14764)
                     /locus_tag="ACG8OL_001722"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS4817709.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14766..15563)
                     /locus_tag="ACG8OL_001723"
     CDS             complement(14766..15563)
                     /locus_tag="ACG8OL_001723"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS4817710.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15541..16245)
                     /locus_tag="ACG8OL_001724"
     CDS             complement(15541..16245)
                     /locus_tag="ACG8OL_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS4817711.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16376..16951
                     /locus_tag="ACG8OL_001725"
     CDS             16376..16951
                     /locus_tag="ACG8OL_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS4817712.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            17069..17746
                     /locus_tag="ACG8OL_001726"
     CDS             17069..17746
                     /locus_tag="ACG8OL_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS4817713.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(17825..19633)
                     /gene="pepF"
                     /locus_tag="ACG8OL_001727"
     CDS             complement(17825..19633)
                     /gene="pepF"
                     /locus_tag="ACG8OL_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS4817714.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(19809..20807)
                     /locus_tag="ACG8OL_001728"
     CDS             complement(19809..20807)
                     /locus_tag="ACG8OL_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS4817715.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(21033..21686)
                     /locus_tag="ACG8OL_001729"
     CDS             complement(21033..21686)
                     /locus_tag="ACG8OL_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS4817716.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         6492 a         4698 c         3814 g         6988 t
ORIGIN      
        1 ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg ctataataac
       61 caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat tcaaaaaata
      121 gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata tcttgtttta
      181 ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat ccatcggcag
      241 gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa aataagcata
      301 ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt gctgaaactt
      361 tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc gatactttcc
      421 cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat actttctatc
      481 agatggaagt agaattccca gttgcttata ttccagcagg ttctggagat gattttgctc
      541 gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta gcagctcaat
      601 caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag
      661 ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca aatcattctt
      721 ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta ttctctattc
      781 tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga ggaaaacaat
      841 taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt ggcggaggaa
      901 tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac
      961 gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa aaacaattga
     1021 aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca acaacaccgc
     1081 aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc acaataacca
     1141 caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta catctggaac
     1201 accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt tttttactat
     1261 gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta cccaaaataa
     1321 attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg aaccaaaaag
     1381 attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta ttttgaacaa
     1441 accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag tattctgctg
     1501 gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg atcccaccgc
     1561 tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg atcggcaata
     1621 aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag gtgtggatca
     1681 ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa ctaatcaaac
     1741 taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg tttcgtttac
     1801 tgatctcttt gcgattaaat gctagattga tgacgctgaa accgctaaaa atggatagaa
     1861 acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc atccgctgac
     1921 tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt gtctgttcca
     1981 tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt
     2041 tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt atcttgatcg
     2101 tcggcatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt aagataatgt
     2161 ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca tccaatgtaa
     2221 cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc gactcttctt
     2281 cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca atattttgcg
     2341 caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc gatttatttg
     2401 ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct ttccatccaa
     2461 gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt ggtataaaaa
     2521 ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa gttgacgaga
     2581 tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg ttccgacccc
     2641 atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct atccttttgc
     2701 accagattca tttttggtat caaattacca accaatgtac ctaacaaaat cgcacacaac
     2761 gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc tacttgatag
     2821 ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt aaagcttgct
     2881 gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca
     2941 tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag
     3001 agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct
     3061 gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca
     3121 acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc
     3181 atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat
     3241 gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct
     3301 acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag
     3361 actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta
     3421 atcgtttcga gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct
     3481 aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt
     3541 gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt
     3601 gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta
     3661 actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt
     3721 agttgttact tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg
     3781 acaacacgtt ttctgggagt aatgggatgt cgatcgtcaa acgatccgcc gtccgtacca
     3841 tatcttttag agattctttg gttcctttag caatcacctg tccttgatcc atgatcacga
     3901 tctggtcgca aagaatttct acttcttcca tgtaatgtgt tgtatagaca atggttgctc
     3961 cttgtcgatt cagctccttg atactttcta aaattttatt ccgactttgg gggtctacag
     4021 ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg ggcaatccca catgcgatgt
     4081 tcaaccgacg tttcaagcca ccactaagtt ctcctggacg aaacgaaaca tatttttcta
     4141 atcccactaa ttgaatcacc tcttcaatca aggctttcct tttctctttt tcggaaacat
     4201 ataaaccgca aaaataatcg atattttctt gcacacttag ttcgtcaaag accgcaacgt
     4261 cttgaggaac gaccccaatc attgccttga cagcataatt ttcaggagtc attgttgtcc
     4321 cgaacaattt aatggttcct ttgtcataat ttaacaaaga aagcatacag ttgatggcag
     4381 tcgatttccc actgccgttt ggtcctaaca gacctaagac ttctccttct tgtacttgca
     4441 gatcaaaatg attcaatgct acaaaatctc catagcgttt gactaattta tcaatttcga
     4501 taatcatcat gaaaaactcc ttccaaattt cttgttgatc tgactataat aaagtgcaag
     4561 aaaagaaact agtgacgatt ggctaaacca cacatgacat ttgtcacaat gaaggagtct
     4621 gctatgcata aatctcggct actcaacgaa actagctttc tactggttat ctcattgctt
     4681 ttgctggtaa gtcaaggatt cgttgctatg caagttatct atttattgat cgccgtttta
     4741 ttcactggtg tcagttattt actgcctaca aagccaaatt ggattttcca agttattcta
     4801 tttcttttat gtgccttttt acctgatctt gtcctgtttc tgccagcgac gatgcgagtg
     4861 atcgcccctg aagaaagcca ccttgattac ttgtttttgt taggtttttt gttgatttcg
     4921 acggttggta atcatccccc catcaaggtc gcctttgtcg taattttgct gtttttttcc
     4981 ttttacttac gaaaaaaaga tcaacgaacg accgatcttg agaaaaagta cattgtctcc
     5041 aaagatactc attgggaaaa acaacaagag ttatcggaac aaaataaagc attggccctg
     5101 tcacaagaaa cacaattagc cttgcaaatt gtggaagaac ggaaccgaat tgcccgagac
     5161 atccatgaca atgtcggtca tctgctgtca agtgcgattt tacaaattgg tgcgcttgaa
     5221 atgatcaatc aagaaccaaa actaaaagaa ccattagaaa atttaagtgc caccgttcac
     5281 actgggatgg atcggattcg tcaaagtgtc catgacctcc atgagacatc gatctctttt
     5341 caacactcct tagagtttct gatcgctgat tttccttctt ctgttacgat tgaaggaaac
     5401 ttgtttgaag gattgaatga aacgcagcaa aactgcttct taatggtcat caaagaagcc
     5461 ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg ttttacattt tcgaacgttg
     5521 ccggcctttt atcgcttgca aatcacgaat gatggcacca gcccacaaaa aaattctgct
     5581 cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag aacaaaaggg acaagtccat
     5641 attttccaag aaaaccagca atttcagtta acgatcattt taccaaggga gggacaccaa
     5701 tgataacagt agtcattatt gagatgatcc atttgtgacg aaatcgttac aaaccatttt
     5761 agaaagcacg aaagagatcc gtgtgttagg cattggtcat tgtgccaagg atgcgctagt
     5821 cttatacgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc ctaaacaaac
     5881 gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc ttttgctttt
     5941 gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg tcaaagggta
     6001 tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt ataatggaca
     6061 ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga ataaaccgac
     6121 gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca tcaaagaagt
     6181 agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg acggcactgt
     6241 tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca cgcaattggc
     6301 tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac ctgaatgatg
     6361 ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg caaaccattt
     6421 cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg attgttcggc
     6481 gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga ttcttcacga
     6541 tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat ctcatctaac
     6601 tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc ggtttcttta
     6661 gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga aaaaacaaaa
     6721 gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca agcatttttt
     6781 gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc tactaactgg
     6841 ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc tacctcttct
     6901 ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc cattacttgg
     6961 tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt tacaacccgt
     7021 ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga atcttcataa
     7081 gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat ccttgatttc
     7141 aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc ttgctcttcc
     7201 cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa attacgctga
     7261 agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc actgccttgt
     7321 gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt tcttttataa
     7381 gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc agaatctttt
     7441 gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa ttcgggagta
     7501 ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca atgtacttcc
     7561 ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa cgcatcgatc
     7621 cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg gaatccctta
     7681 tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc atcacttcgg
     7741 aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc ttctttgatc
     7801 tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg attgagttcc
     7861 attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg accgacagag
     7921 ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat cgctttgaat
     7981 aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa aagtccgcct
     8041 tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc cagaagacga
     8101 tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc tcggccagtt
     8161 ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc caattccagt
     8221 ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt ccccgtacct
     8281 ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc ttgttcttgc
     8341 gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt ctgcacttct
     8401 ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga attaccgatc
     8461 ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg ttgtatcttt
     8521 ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat ttcaattggg
     8581 cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta
     8641 tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc
     8701 ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt gaacccgatt
     8761 ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa agactcgttc
     8821 ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg attcagtccc
     8881 atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat ttctcttttt
     8941 ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat tttttcgata
     9001 gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat tcctgggaat
     9061 ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc ttgctcatat
     9121 ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc ataaaaacga
     9181 taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc tttttcacgg
     9241 taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt actaggattt
     9301 tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg ctgcatcgga
     9361 agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt
     9421 ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag tctctaaaat
     9481 cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct cttttcctgt
     9541 catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa gtgggccatc
     9601 tttatgttgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg ccgattcaat
     9661 cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca aattcggcag
     9721 attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag tacccagccc
     9781 tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct ctttgttgat
     9841 accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt taaaaggaac
     9901 atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca aaagcggaga
     9961 atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc catgtcgggt
    10021 aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc tataataaac
    10081 tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag ggaatccttg
    10141 ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca atgagcggac
    10201 atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc
    10261 tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat cagcaaaatt
    10321 ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac gattacctaa
    10381 aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat tccaataatc
    10441 caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct cgattaaatg
    10501 aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg cagggattaa
    10561 tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta tgttcgtttc
    10621 tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt gtcagtcact
    10681 gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag gaacctgata
    10741 aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt tttgcaacct
    10801 ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat aattctatgt
    10861 caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc tcttctacct
    10921 gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt atctcaaatt
    10981 cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg gctactacac
    11041 gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga cgtagttcct
    11101 ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag agtacacgtt
    11161 ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag
    11221 caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat tgttctgtag
    11281 ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc tttttcaatt
    11341 ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc gagacttctt
    11401 caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat tggatcattc
    11461 tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg gataaaaaat
    11521 aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc aagccattat
    11581 cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga ttttctgcca
    11641 aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc tgttttatct
    11701 taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct agaagaaagt
    11761 gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact acacatctat
    11821 tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact tagcggtcgg
    11881 tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg gggaaaaaga
    11941 tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata acgatatcga
    12001 attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg atggcgtagt
    12061 atttggttgt cttactcctt caggctggct agacgaagag gccttagaac tattgatcga
    12121 aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc ctgaagatcg
    12181 gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct tgactcatgg
    12241 cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat tgattgccta
    12301 tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga atgcagcaca
    12361 tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg atcttcagtt
    12421 ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca aatgtttttg
    12481 tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat cagtacactc
    12541 gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc aaatcctaga
    12601 cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat tgcaaatagc
    12661 atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc tacgattaga
    12721 aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc agccaaaaca
    12781 agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac agccacagct
    12841 agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa gactgctaaa
    12901 atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt cgacatacct
    12961 aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac tggattttca
    13021 ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc ttcatcatca
    13081 ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc ataatcggta
    13141 acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg
    13201 cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc gtcgtcatca
    13261 ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg attaacgaca
    13321 tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta catcgcagag
    13381 cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc gccagccgtt
    13441 tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga catcaaactt
    13501 aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc attatctgta
    13561 tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag gtattctttc
    13621 atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt aggcgacaga
    13681 taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg cccctgctca
    13741 tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc ccttaaatct
    13801 tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc aatctcacct
    13861 cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa ggctgcttga
    13921 actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa tgaagggctt
    13981 gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc agggcacagc
    14041 caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc tggatatcaa
    14101 ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc gaacgtcctt
    14161 catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc aaatcgatat
    14221 gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat tttttcacaa
    14281 acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa
    14341 gcatcaaacc agatgtatcc cgatctagtc gcgtcaccac atggattact tggttttcgt
    14401 aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt ggatggtatt
    14461 gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt tcatcttcat
    14521 acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct tcagcaggaa
    14581 tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct acttgatcat
    14641 taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt gagatccctt
    14701 tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc catgtaaact
    14761 gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg catatgtcga
    14821 taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg gatatttgct
    14881 tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc caattttatt
    14941 tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa cactcgatta
    15001 ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat cacagcaccg
    15061 ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct
    15121 ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt acgattggcc
    15181 cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc atccaaataa
    15241 ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt tcgtaagctg
    15301 tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa atgacctgta
    15361 tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa cgccgacaat
    15421 aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc tcgttgaatc
    15481 cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt tgcttcttta
    15541 ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt tcttcaaaag
    15601 cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc gctgtgcttc
    15661 ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag cttctgccgc
    15721 acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat aattcagcga
    15781 atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt ccgccaaaat
    15841 tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat aagaacggta
    15901 gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca tgtcttttct
    15961 tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca tgatccttaa
    16021 cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc gtaattgtga
    16081 ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa tcgggacatg
    16141 tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca attctgatac
    16201 agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg atacctctac
    16261 ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta ctcttgattt
    16321 atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg atcaaatgac
    16381 acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg agatattcat
    16441 cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt atttcgatac
    16501 cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg ttggtcatca
    16561 agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa cggatacctt
    16621 tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt
    16681 ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa ccggaaagct
    16741 tacaacaaaa cgggctgaat tccctattga agaaggactt ttagctattg atgaaagctg
    16801 gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat ctgtgggaaa
    16861 aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg ctaagaacaa
    16921 gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa ggcacttgag
    16981 ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag tcactagttg
    17041 tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg ttaatccttt
    17101 aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag aagagtacgg
    17161 gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat
    17221 tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac gtaatcactt
    17281 attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa
    17341 aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg acataccata
    17401 ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga atatgtttaa
    17461 agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa aaattgctcg
    17521 ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct gcgaccgtga
    17581 ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca cagagctgtg
    17641 taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata caaatgattc
    17701 tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaaaa atacgtcgac
    17761 tcccttacaa gagagccgac gtattttttt cgtttctatt cgagaaaatc aaaaatagaa
    17821 agccttataa acgatcgatc aattcttcta attcatttag acgttgttca aacatcgaca
    17881 ttgcatcttc aatatatgca gcctgtgtca tatcaactcc tgcttttttc attacttcca
    17941 caggataatc gctgttacct gcttttaggt aagccaagta gttctctagt gcctctggtt
    18001 cctggtttag tattttcttc gccagtgctg aagcagcaga aaagccagtc gagtattgga
    18061 aaacatagta attataataa aaatgcggta tccgtgacca ttcgaattta atttctgggt
    18121 cttcttcgac tgctggacca taatattttg cattcaattt accataacta tcacttaggt
    18181 attcactagt caatggcaca cctttttcat cttccgtatg catgaaatgc tcgaattcag
    18241 cgaattgtgt ctggcggaaa actgttcctt taaacccatc caagtaatgg ttgagtacat
    18301 aagcccgtac acgagggtct ttttctgttt ccaataaata ctccgttagg atattttcat
    18361 tcgttgtcga agcaatttct gctaaaaaga tggagtagtc gccgtacaca taaggctggt
    18421 tcgaacgagt gaaataacta tgaacactat gtcccatttc gtggacaagc gtaaatagct
    18481 gatccagcgt atcatgccaa ttcaataaaa tatatggatt tgtgtcatag cttcccgaag
    18541 aataagcatc gcttcgtttc cctttatttt cgacaacatc gatccaacgt tcagagaatg
    18601 ctttttctac gatggccatg tattcttcac ccattggttt cagtgcttct aaagcttttt
    18661 cttttgcttc ttcgtacgta aaggtaattg gagcttcccc taagaccggt gtataaagat
    18721 catacatgtg cagtttttcc acttctaata aacgtttccg taattccatg tatcgatgta
    18781 acaaaggcaa atgcttgttt accacgtcta ccaaagtatc gtatacactt tcaggaatat
    18841 gattattgct caaagacgct tctctggcag agctgtaatt acggactttc gctttaaaat
    18901 tatgtccttt tatatgtgtg cctaaagtag aagcaaatgt atttctaaat tgttcgtaaa
    18961 cactgtacaa tcccttaaat gctgcttcgc gcacccttcg atccgtgctt tctagcaact
    19021 gaccatacac gccatgagat aattggacta tttcaccatt ttctccttca atcgttggaa
    19081 aaactagatc tgcattattc aaaacagcaa atgtatcact tgaagcatca aagatttcac
    19141 ctgctccagc aagaagagat tcctgctcag cagataagac atgggctcga ttatctacta
    19201 tttgctggat ataatggcga tagacttcca attttggttc ttctttgaaa tattgccaaa
    19261 tctggtcatc tgacaattgc aatatttctg gttcaaacca ggaaacggct tcactaactt
    19321 tcgaaaacag actgcttgct cttgcataaa gcgcttggta atctgtattt ccagtgtctt
    19381 gatcgttttt aaggtgcgca tatacataaa tgacttcagt ttggcgataa accctcaata
    19441 cgaattcaat cgcatttaaa aattgagaag cgccttgatc aagtgttcct ttgtgttttt
    19501 cagattgttt taactcttct gataattcca aatatttctc atcaaactct tggtcgcttg
    19561 aaaagatctt ggtcaagtcc caagttaaat tttcaggcaa ttcttctcgt tttggcaact
    19621 gctttacttc catgaaatcc actccttttc ttcccttagc tctatctatc aaaatagatc
    19681 atagtctaac atccacatgt cacagcatct aacttttcta ctcattcatg tgtctgcttc
    19741 catcaatttt agcataacta aatcttgttt tacatagaaa accgattcct agcctatttt
    19801 tatacttact attttttcaa agaagacaag tgtatgcatt cgttaaaaaa ctgctgatag
    19861 accatctctt catcaatcat aggaaaaagc cattcccttt tatgttcttt tatttggcaa
    19921 agccatgagt tgaaatcagg aactttctgg ttttccaagg cttcttcgta caacattcga
    19981 taaagaaaaa cctgttcttg taaataaaag aaaaaccgac ttttttgata gatccacgat
    20041 gatagatgca aaagatgttt atgacgcaag tagcattgtt cttgtatgga tactgtattc
    20101 ttttgcttgt gaataagttg gcgtgtcagc catttcttcc catcttcact cactttttct
    20161 atttttttca tttggttcat tttaatttcg ttactagtga atatttcttt aaggtcccta
    20221 ctagaaaaag tccagcagct actgcaaaag gaaatatgcc ctgatacagt ctctgtaata
    20281 tgatgataca aataaagctt ttgtttcttg agatcagctt tccagcaatg cactcctctt
    20341 tttctattat agtagcagca acttttttct atcaatgaga attgcgattg tccaagaaaa
    20401 gaattaccaa gtatccacca tacttggtaa ctgttattca aataattttg ggttctttca
    20461 ataaatctct ggtgagacag tctagaacat tggatttcta ttgctagatt cgccaacaaa
    20521 atatccggcc tttgccgcaa cctcggcaaa taggcttcca aaaatactgg ttctactgac
    20581 tgtcccagcc aatccatgaa gcattccttt aactgtaagt gctcttttga ttctctttca
    20641 ctaaagaccg aacagttatc tgatttttga tgggcaaaat gagcacattt tttctttccc
    20701 tttttcaaaa tcactctttt tcgacagcca cgacaataat atttctttcc tttttctgca
    20761 tgtttagcaa aacagcactt attatattca ttcattgcaa gcagcatttt tctccctctg
    20821 aacttattta cgtctaagaa tttatttaca cgaaaaaaga gggcgtgaaa aagctattct
    20881 gcatcaagta ataagaacag ctaaacaaaa atagttccca atatttttcg tttggttggg
    20941 cttgttaccg cagatgcaag cttttgaaca catttatacg aaaaaaagac tgcgacacaa
    21001 tttttgtcac agtctcttac tataatgctg atttatacaa aataaaattt cgttcgctcg
    21061 atagcattac gcgccatcag acattgtcca tattcattca atgtttctgg tgtacgatcg
    21121 ctcttttcag caaactccaa aattcgagca atctgattct ctacatccgc ttggttgaga
    21181 ttttccatcc agaagtgaac gactaggtaa tagcgctctt cgaataaata caaatcagtc
    21241 caagcagact ctagttcgac atctgttgca agttggatca cttgttcaaa atcttcaaaa
    21301 gcaaaagttg cttgtctttc ctgttcttct agggcgcttc cagataattc ttcctccgga
    21361 tcgctgccaa taatctgctg acgaagaaag tcactcacat cttctgaatc aacagtagaa
    21421 tcttctgatt gagaaatttc ttcatccgga agatttttac tgataaacag ttctaatccg
    21481 tctccttttg gcaaaacttg gaaagtgaca gcttcgcttc ctcggaactc ctcttcgata
    21541 tcaacttctt ccaaaatact ataaaagaaa ttttcgattt ctttgtgatt gccaagtaaa
    21601 tccaaaaatg aaacgccccg ttcctctaaa tcttcatgac caattagtac acggatcgta
    21661 ttttcattaa tatgttccat ttccattttg ctacacctca ctttgccaca atgtttaata
    21721 tagattcatt gtagcgaaat cagcttaaat gtaaagaaaa aaaatctcgc tgttaagcga
    21781 atttaataaa accagcggat ttttcaacct ttcaagctat cttatttaac tataacattt
    21841 ttggcagacg aatactatat agccgacttt tcagctaatt ttccctctct atttttcgtg
    21901 caatcatcat cttctactac ctttttatac atctaatcaa gaatactttt tgtaaaaaca
    21961 aaaaaacttg gaaaaactat ctttgtaata gt
//
LOCUS       DAZUJO010000044        21508 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000044 DAZUJO010000000
VERSION     DAZUJO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.045"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..889
                     /locus_tag="ACG8OL_001730"
     CDS             188..889
                     /locus_tag="ACG8OL_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4817717.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1498..2514)
                     /gene="tsaD"
                     /locus_tag="ACG8OL_001731"
     CDS             complement(1498..2514)
                     /gene="tsaD"
                     /locus_tag="ACG8OL_001731"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS4817718.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2511..2975)
                     /gene="rimI"
                     /locus_tag="ACG8OL_001732"
     CDS             complement(2511..2975)
                     /gene="rimI"
                     /locus_tag="ACG8OL_001732"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4817719.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2982..3524)
                     /gene="rimI"
                     /locus_tag="ACG8OL_001733"
     CDS             complement(2982..3524)
                     /gene="rimI"
                     /locus_tag="ACG8OL_001733"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS4817720.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3508..4230)
                     /gene="tsaB"
                     /locus_tag="ACG8OL_001734"
     CDS             complement(3508..4230)
                     /gene="tsaB"
                     /locus_tag="ACG8OL_001734"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS4817721.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4421..5401)
                     /locus_tag="ACG8OL_001735"
     CDS             complement(4421..5401)
                     /locus_tag="ACG8OL_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4817722.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5425..6909)
                     /gene="galT"
                     /locus_tag="ACG8OL_001736"
     CDS             complement(5425..6909)
                     /gene="galT"
                     /locus_tag="ACG8OL_001736"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS4817723.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6921..7910)
                     /gene="galE"
                     /locus_tag="ACG8OL_001737"
     CDS             complement(6921..7910)
                     /gene="galE"
                     /locus_tag="ACG8OL_001737"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS4817724.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8158..8325
                     /locus_tag="ACG8OL_001738"
     CDS             8158..8325
                     /locus_tag="ACG8OL_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS4817725.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8387..10198)
                     /locus_tag="ACG8OL_001739"
     CDS             complement(8387..10198)
                     /locus_tag="ACG8OL_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS4817726.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10195..10560)
                     /locus_tag="ACG8OL_001740"
     CDS             complement(10195..10560)
                     /locus_tag="ACG8OL_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS4817727.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10723..11118)
                     /locus_tag="ACG8OL_001741"
     CDS             complement(10723..11118)
                     /locus_tag="ACG8OL_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4817728.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11136..12098)
                     /locus_tag="ACG8OL_001742"
     CDS             complement(11136..12098)
                     /locus_tag="ACG8OL_001742"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS4817729.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12098..12310)
                     /locus_tag="ACG8OL_001743"
     CDS             complement(12098..12310)
                     /locus_tag="ACG8OL_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS4817730.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12331..13029)
                     /locus_tag="ACG8OL_001744"
     CDS             complement(12331..13029)
                     /locus_tag="ACG8OL_001744"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS4817731.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13049..13591)
                     /locus_tag="ACG8OL_001745"
     CDS             complement(13049..13591)
                     /locus_tag="ACG8OL_001745"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS4817732.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13723..14727)
                     /locus_tag="ACG8OL_001746"
     CDS             complement(13723..14727)
                     /locus_tag="ACG8OL_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4817733.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14724..15713)
                     /locus_tag="ACG8OL_001747"
     CDS             complement(14724..15713)
                     /locus_tag="ACG8OL_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS4817734.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15710..16516)
                     /locus_tag="ACG8OL_001748"
     CDS             complement(15710..16516)
                     /locus_tag="ACG8OL_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817735.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16682..17638
                     /locus_tag="ACG8OL_001749"
     CDS             16682..17638
                     /locus_tag="ACG8OL_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4817736.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17715..18233)
                     /locus_tag="ACG8OL_001750"
     CDS             complement(17715..18233)
                     /locus_tag="ACG8OL_001750"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS4817737.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18321..18470)
                     /gene="rpmG"
                     /locus_tag="ACG8OL_001751"
     CDS             complement(18321..18470)
                     /gene="rpmG"
                     /locus_tag="ACG8OL_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4817738.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18698..19144
                     /locus_tag="ACG8OL_001752"
     CDS             18698..19144
                     /locus_tag="ACG8OL_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS4817739.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19339..21234)
                     /gene="asnB"
                     /locus_tag="ACG8OL_001753"
     CDS             complement(19339..21234)
                     /gene="asnB"
                     /locus_tag="ACG8OL_001753"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS4817740.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6475 a         4561 c         3702 g         6770 t
ORIGIN      
        1 tgttttttat acacaaatca ttcgcttcaa aacaaattat tcaataccat tgtatatttt
       61 ttacataata acaaattata tttgtaaagt attaaaactt gtttctaatg ttggtatgtt
      121 aaaactttac aaattcttta aaaaaagtga caataaatat agtgaacaat aaggagagga
      181 ctttttaatg cgtgttaata aaatcgattt tcacttttca aaactcagat atcaacctga
      241 ttttgaacat ttctcggatg aagagttcca acagatcaag gaacatactg ttcttcgttc
      301 ttataaaaaa ggacagatcc ttttcgacga tggtgatccc agacaacggt tgtatatctt
      361 gaccaaagga ctcgttcgtt tagaacgtta tgatgaaagt gcaacttatt actactatga
      421 ctatgtgaat acaaataacc tttttcctat gtcaggaatg tttggagaag agacctacac
      481 ttatacagct caagcgatga ctgatattga aacattctac cttcctgttt cctactatga
      541 aaaattagca cgaaaaaatc ccgaacagtt gatctatatg atacaaaaaa tttctaaagt
      601 agttgaaata catgaattgc gtatccaaat cggattgacc tctagcgcct ttgaccgggt
      661 aaaacaaaat ttattcattt tgaaagaaga attgggcgta ccaacaaaag atggtcatat
      721 caccattcct tatccgatca ccttaaaaga attagcagtg aatagcggca cgacacgtga
      781 aactgctgga caagtaatca aacaactacg tgattccggg ctgttagatt atgacaaaaa
      841 gcgttttact ttctgtaaga cagctactac tccagctatg agagaataac gcgaacaaaa
      901 ataagcagtg accctgataa atatcagggt cactgcttat tttcctccct aaaacttctg
      961 aaagatccgg cagttttaga tcactggaca agactcaaag attttctatg tgtgtttcag
     1021 ttatgtaata ttcactgttt gcaaaaaata ctgttagatt aactagatga agcgtcttgt
     1081 cctcatccga tctagattat tcgatattcc tcgacaataa acgattactc agtgagggca
     1141 atcagaggaa cggataatga taaataagct gtaaaaaata gcaaaataac aataatgatt
     1201 aaaagtatgt aaaaaatagg gctttgacaa aaacgtgtca cagccctatt tcgattaaac
     1261 ggtgtgtacc tgtcaactct tcggtcacaa gtcataatgt tcataaacat gaggagctgt
     1321 tttttgaaat tgttccttgt gaatcagagt taaacgacag gcttacgaac tcttgattaa
     1381 acggcgttca aaagcttgca tacgcggtaa taagaacagc caggtcaaaa acatgagaag
     1441 ccgtttttcc tgggctgttc ttattacttg atgcagggca gctttttcac aacctcttta
     1501 ttcagataaa accaagcttg gatcagcatt caactgatag ctagcaaaat ttcttttttg
     1561 catttccaca tgtgctgctg ctccaatcat tgcagcatta tctccacaaa gtcgaagagg
     1621 gggaatcact agttctacct cagggagttt agctgccaaa gcatcctgca atccttcacg
     1681 caacccttga ttggccgcta cacctccagc tataatcagt tgcttgactg gataattttg
     1741 acaagctcga agtgttttag taatcaatac atcgatcaca cttgcttgaa agctagctgc
     1801 taaatcattt ttgtctagat cttctccacg ctgttgtgca ttatgcacca aattgataaa
     1861 tgcacttttt aatccgctaa aactgaaatc ataattatct tcatggatca tcgctcgcgg
     1921 aaaatggtaa ttatcttttc cctgatgagc tagctgatcg atttcttttc cgcttgggta
     1981 agaaagacct aatactcttc caactttgtc ataagcttct cctgctgcgt catctcttgt
     2041 ctccccaatg atttcatatg atccatcttc ttgcatatag actaattctg tatgtcctcc
     2101 gctaacaaga agtgccatca atggaaactg gaatggttta accagacggg ctgcatagat
     2161 atgccctgcc atatggttta caggaataag cggcaattgg tgtgcccaag caaaagcctt
     2221 agctgcagaa ataccaatca gcaacgagcc gacaagtcct ggtccataag tgacagcgac
     2281 tgccgataaa tcttctgccg acacaccagc ttctaccagt gcatcttcca agcataaggt
     2341 gatttgttct acgtgatggc ggctagctac ttcaggtact acgccaccaa aacgtttatg
     2401 actgtttatt tgagaagcaa cgatgttaga taagatctct gttccatttc taacgaccgc
     2461 tacactcgtt tcatcacaac ttgattcaat cgccagaatc aattcttcat tcatttcatc
     2521 gtctccgttt ttaactttct gctcataatg atcgcatctt ccgtcggatt gcgataatat
     2581 gatttccgtt tacctaatat acgaaacttt tctgattcat aaagttttct tgctgcttca
     2641 ttagatagac gtacttctaa aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt
     2701 aattgtaaca aatgccgtgc atgccctttt ccttgttctg aaactgcaac agcaatattt
     2761 gtgatctcga cttcatctat tactttcgag tatccaataa agccacagat ctggttatct
     2821 tcagctaaaa gcagataatc agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc
     2881 gtccaaggtg aaccatgtgg ataagcattt tcgctaatca tccatacgca ttctgccaag
     2941 tacgcttcat caaaatcttt ttttctgcaa atcaactaat ctcactcatt ttctaaaagt
     3001 tttatcatat ccaacgcatc ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt
     3061 gcttgaaacc ccaatttccg ataaagtctt tgtgcatctt gattactcat acgcacttct
     3121 aatgacatcg tctcacagcg attcattata gcaaagttct cgatttcgtc aataagcagc
     3181 gaaccgattc gtttacgctg atatgccggt cgaacggcaa tattcgtgat atggcaatct
     3241 aaaccgaacg ctcttgaacc gataaaacca atcattcttc cattatcaaa gatacctaga
     3301 tatagatgag gaagcggtga cttcaattct gatataaagg cactttttgt ccatggcaat
     3361 tcgccattat agacttctcg ttcgacatcc agcagctctt tgatatcttc tgtagtcaat
     3421 tctcttagta cgtaacgacg atcgatttgt tcgactgttt tttctgtata aatcatttta
     3481 tcttgaaaaa agcttctggc atacgctcta aattttttca acataacttt cgtctccagg
     3541 tgtgtgcgtc tccaaccatt tttcttctgc ttctactcgt tttaaatagc gaggaagaaa
     3601 agcttgtttc tctgcaactg gttcaactaa agcacctaat tgagccacag ttacaccact
     3661 tggaatatcc catgctggaa ctgtattaat catcgcacta ggcaatattt cctttatttc
     3721 agagaagaat ttgtgacaat cactcccgac gaaataaata gcttcattca gcttttgtag
     3781 ttgtgtgaac aaatcagaag cagaaatatg tacatcttca agtacagttt ctaatctatt
     3841 ttctttccag cggtatgccc cagcgtatac attttttctt cgagcatcaa acatgggcac
     3901 aataatatta tccacaccga tgcaattagc cgcgatggtt ttcagactag atactcctac
     3961 tagctcttta tctaatgtat aagcaatcgt ttttgctgtc gttacaccta tacgaagccc
     4021 tgtataagaa ccgggaccat cagaaaccgc gattcggtca atgtcttttg gacttatttg
     4081 aagatctgca aacaattggt cgattgcagg catcagtgtt acactgtggt tgcgtttgat
     4141 atttgtttgt atttgtccaa gaatttgttc gtcttctacc acaccgatcg ccaaagtttg
     4201 gtttgcagta tctattccta atgtaatcat atatttgatc ctttctgcta tgacattctc
     4261 gccttccatt gtagcatact tttttattct gaggatgaaa ataccaaagg caaaactagt
     4321 acaataatac atgattatct gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa
     4381 gctgcggtat cactccattt tcccacagct tttccatctt ttatttcgtt gattctcgaa
     4441 taatcaactc tgtttccaca ataatctttc ttgaaaccgg tgctttggaa ttacttaatt
     4501 gaagaatcgt ctccactgct agctctccca tccattcggt ataaactttt acagtagaaa
     4561 gcgagggcgt tacatatttc gctacactga catcattgaa gcccatcaca gaaatatctt
     4621 ctggtaccac taaacctttt tcttgaatcg ctttcagtgc gccaatcgct aatgcatcat
     4681 tagctgcaaa aagcgcatca ggaacatgcg gattcgtttt taaatattgc ttcactgctt
     4741 gatatcctga ctcgacagaa aaatctgctt cgataattgg accttcgtcg tagccttttt
     4801 ctgccaaaac ttgttgaaat gccataattc tgggatcttt tagccgctga tggtttttct
     4861 tcgtatattc tgttccagca agcatagcga tatgttgcgc tttatgggag acaaagtgtt
     4921 caatgacttt ttgaacactt gacttaaagt cgacaactag agagtcgatt ccttgagaag
     4981 tgccgtcaga atctaccaaa agcaaggcat tttgggatcc tttcatttct aataactgag
     5041 cttcgtcgaa ttttcctagt gcgatcgttc catctgccgg aagatcacta atggaatccc
     5101 acgtttcttt caataaagta acgtttgctt cttctgcctt tttttcgatt cctaggcgaa
     5161 tagataaata ataaagatct tcaagctctt ccgtatcgtc gtaccactgg atcaggcgaa
     5221 gcgtttgctt agtatttgct gcatttttac gatgtttcgt ataatttaag cgttctgctg
     5281 cttcaaaaat tttttgcttt gtggctaagc caacggaaag atcaggatca tagttgagaa
     5341 ctcttgaaac agtagcagga gaaactcctg ccaactgcgc aatgtcttta attgttgcca
     5401 tttgtctctc ctcctttttt acgtttataa aatcgcagta aatcgttcaa acgctgcctg
     5461 tcctttttca tctcgcttga atactccagc gtcttccagt acacgtgcaa aaacctgacc
     5521 tacagatttt tgtacgacat cggtgaccgt atctttcgtc aaatcaggat gttctttttt
     5581 taattgttct gcccacggac gatgatattc agccatctgg ttttcttctc ctaataagaa
     5641 atgctctact tctactagtt ctggtttcaa acgaggtggt aaaacagcaa gccccattac
     5701 ttcgatcaac ccaatgttct cctttttgat atgttggaca tctttatgtg gatgaaaaat
     5761 cccatcagga tgttcttctg aaacattgtt gtctcgaagg acaaggtcca actcaaagta
     5821 attgtccctt cttcgtgcaa ttggcgtaat cgtatggtgt ggtgtaccat ctgctgaaaa
     5881 agctctgata gatactgttt catccgaata tgctttccat ttttccaata ctgagttagc
     5941 tgcttcaata agcaagtttt tctctgttga ttgcaaacgg atcacagaca tcggccattt
     6001 cacgactcct gcgatgactt gcggataatc ttctagtttg ataagttttt ctatcggtgc
     6061 ttttgccata ggaaattcat gacgtcctgc ctgataatga tcatgagaaa gaatcgatcc
     6121 tccaacaatt ggtaagtcag cattcgaacc aacaaagtaa tgaggcaaga cttcagtaat
     6181 ccgaagcagt ctagcaaatg tttcctttgt gattttcatc gggcggtgtt gttctgataa
     6241 aatgatcgaa tgttcattgt aataagcata aggggaatat tggaatcccc agctttcacc
     6301 gtctagattc attcgaataa tccggtgatt ggtgcgggct ggatagttca agcgacctct
     6361 atatccttcg ttttccatgc aaagcatgca ggtaggatag tctactttta ccgcctcgcg
     6421 ttctgctgcg atttgttttg gatctttttc tggcttagat aaattgattg tgatttccag
     6481 ttcaccatac tccgactctg ccggaaattt gatgtttttt gcgatggctc ttgttttgat
     6541 gtagtcattt ttcttactta aattaaagaa atactcagtt gcttcctctg ggcttttcga
     6601 atagtgctgt gcaaagaatg cattgacgac tgaaggtggc ggtgtgagaa aatccattaa
     6661 ttgcgcttca aaaatctctt gttctgctgg caattctgaa agaacaccat tttctcttgc
     6721 cttagcgaca agctgatcca ataagtctac agattcttcc ttaactggtc ttacttcaat
     6781 gctttctaga gcatcttctc ctaccatccc taaaagacga ttctgtaaat ataagcggtc
     6841 catttccatc catccgccag cttcgatcgc aagtgtggca aaatcacaaa tcgtttgact
     6901 gattgacata tctcattccc ctattcttca taaccatttg gatggctgac atgccaatcc
     6961 catgctgttt tgataatcgc tttgatatct gtgtattcag gtttccagcc cagaatctct
     7021 ctcgcttttt cacttgaagc gactaatcgg ctaggatctc cagctcttct tggtgctatt
     7081 tctgctggaa tttcttttcc tgtcacttct ctagctgctt ctagcatttc cttgactgag
     7141 tatcctttgc tgctgcctaa attgaagaag ttgctttcat ttccttcttt caagtattcc
     7201 aaagctaaaa tatgtgcagc aatcaaatct tctacttgaa cataatctct gatacatgtt
     7261 ccatctggtg tatcataatc gtctccataa acagccaagg cttttctttg accaagagct
     7321 acttgtaaaa tgattgggac aagatgcgtt tctggtgtat gatcttctcc gatagatgca
     7381 tctgcttttg ctccggctac attgaagtag cgcaaagcga catagcgcat gccatatgct
     7441 tggtcacacc acttcatcat tttttccatc atcaatttac tttcaccata cggattttta
     7501 ggattagctg gtgtatcttc tgtgattgga gactctttcg gctctccata agttgctgca
     7561 gtagaagaga atacgatttt attcacgttg aactcgtgca tgacttcaag taagatttgc
     7621 ataccgtata cgttgttgtt gaaatacatc aatggttttt cgacagattc tcctactaaa
     7681 gagcttgcag caaaatggat tactccttca atcggttcct tttcaaatac ggaacgtaga
     7741 aattctttat cccggatgtc tccttcataa aaatgagcat ctggatgtac tgcttgtttg
     7801 tgacctgtga gcaaattatc taccacagct acttgatacc ccttttgaac tagctggtct
     7861 acagcgtgcg aaccgatata accggctccg cctagtacta aaattgtcat aaaaaacact
     7921 cctttgatta gtaaaattgt gtttgttgat tctatataat ttaaaaaacg aagtttactt
     7981 ttatggtgaa aacaaacaaa gtaaaatttc gtaaattcat atggtaaatt tataataaaa
     8041 catttagtaa acattttcaa gggttttttg caaaggttta caaacaaacg taaaactagc
     8101 ctttttttct cttttgttcc ataatagata taacaaataa taaatgaggt ggattcaatg
     8161 aaaaaatttg tatctggaat attagtagga agtctggcaa cagccgctgc cgttgctggt
     8221 ttggttgctt ctgtgaaaaa aacagtgatc gatccaattg acgaaaaaga agcaatgatc
     8281 gaagaaaacc gtaaaaaagc aatgcgtaaa cgcgtttctc gttaataatt atttaaaaat
     8341 acgcatgaag actttctaaa aaacagataa gtcatcatgc gtatttttat ttgtcagtca
     8401 atataagttg tgctatttta tctgcctgtc tcagattcca tggatctgta taaggtctag
     8461 ctactgctgc agttagccaa tccacacctt caacaggtat tccatagcaa taaaagtcag
     8521 actggatttc ccctgaagca tcaatcacgc gattcgtacc tcgctcaaca tctatagctt
     8581 ctgaatgata acttcctgta ttatctgaaa atgaaaaagg gcgaacaagt ttgttttcct
     8641 tcaaattttt tatgacagga ttacttgaac gagaaaagct tgtttgtggc aatcgagctt
     8701 caatcaaata gcgactgctg atttctttat cttgtagaga aataacatag cggttgtgct
     8761 tttgctgaat atcaaatggc ggttcgatca aatgaaaaat acccgcatct atcagtgctg
     8821 ctaattcttt cgtccgaatg attggcggac cgatcgttaa aaagctattt agatgagtaa
     8881 accatcccca tagtatttct ttgtattcct tagcagaaaa aacgtgccat tcaatagcct
     8941 gccggattgg atcacgccag tcttttaatg catcaaatgt tgaagcaaat gctcctgtgc
     9001 aattgccttt ttccgcttct tttatctgcc cttcaataaa tattcggcta gcttctgaaa
     9061 agggctcatt ctgataggca agaggagttt ctaaatacga ccaatcccat ctataaggga
     9121 ttagtgctgg atgctttccg agacactcct ctgaagagag ggagagaaaa tcatgctcaa
     9181 aatttttctt tggaatcggc aagtgatatt cttcgatgat tttcttgtaa taaaacaact
     9241 caacgtcttt tttcagatag tcaaaaaatg tttcgccagt caaaaatccc gactgatgcc
     9301 attgctccag gttttctttt gttagtaacc gtggccatgc cattgctccg ccttgctttt
     9361 gatttcttcc tcgagggtaa taagggagcc cccttcttga tccactgtat acaatcggtt
     9421 ccttgcctga tggatgatag accagttttc ccttttcgtt ctcgaagcgc cctcctcttg
     9481 atactgtaaa caatccgaca taatcaaaaa aacttaagcc tagacctctt aaaaagacat
     9541 tttctttagg cggtatcttc gaaagatcta catctgcagg attagaagga agctgataca
     9601 gtaaaacatt tttctttgcg tagtctaccc acactctttc ttcttctgtc tcatcatttt
     9661 cccagtgacc gcttgctaaa acaagctgat cgatagaata gctttgctga tctgtctgta
     9721 aaagataacc atcttttctt ttgcttactt tttttactaa agaataaaca aactgaatag
     9781 aagctggaaa ttcttcttgc aactttgaga aaaaccagcg ttgatagagt ccgtataaac
     9841 atcgagtaga ttgctgatct ttttccagac gattgatttc ttctataatt agttgcttat
     9901 ttttggttac cgtctgttcg atataatctg tagcaggtcc tttactccat tgaaacaagt
     9961 tgggtccctg tgttaccaat cctccactgg acaatgtttc atctgtaaaa agcgtcactt
    10021 gttgagaaac agaattcatt aataactccg gtggctgatt caaccgccaa actcgacctc
    10081 caggaccatt gggatcgaaa agaaaaagtt caagagaatg tctgtcctga ttattatgga
    10141 gaatcctttc tgctacactt aatcctctag gtccggcacc aatgatccct attttcattt
    10201 gcatgcctcc atttcgataa attttttcaa taaaacagca tggttctgat gttcattttt
    10261 tgcaccataa acaaaagtaa tgactggatg ttctttcata agttgactga gtttaaaaat
    10321 agcttgcttc ttttcttctt cgaaaatcaa ttcttgctgg tatttctttg caaatgaagc
    10381 aaatcgttct ggtacatggt caaaagtttt gcgtgcttca ttactaggtg caatttcttt
    10441 caaccacaga tctaactgcg ctttttcttt tgaaattcct cgtggccaga atctatccac
    10501 gagtattcta tagccatcct ctttttcagc atcaaggtaa acacgtttta atttgatcat
    10561 agttgtttcc ttctttctac cttttcgctt agcttatata tgtagataat agcacaaaat
    10621 aaaaaaaatg aacggcacaa ctatccattg cgccgttcat gtgatcttgg tcaaaatcta
    10681 atgattgatt cggtttttaa agctgtcata caatcatctg ttttatactt ttttactttt
    10741 gatttttcca gaccatccgt catatccacc cttaagtaaa taaatctctt gataacctgc
    10801 tttacgtaat ttatttgctg tacggatgct caaacttttc ttttgatcgt aaatatagac
    10861 aggctggtct tttcgaatag atcctaatga atctttcaaa acagtgtaag ggatgtttct
    10921 tgcacctaaa atatgcccag catcaaacac atctttttcg cgaacatcga ttacttgtgc
    10981 ttttctcatt ccttctcgaa attcttcttc tgtcaaagtc gttgctgaac gtttggccat
    11041 tactcgtaga tagacttccc aaaggatgat tgcaagcaca atcaatagca aaatgatatt
    11101 aattacccat aaaaccatgt aatgcctccg tttttttaag caaatttcaa ggctaatgag
    11161 gcagcaccaa tgacaccagc ttcattacca agctctgcta atttgatttt tgttgattct
    11221 cttacttgcg ggaaagtaaa ttctttaaaa tatttctcta cacggctacg taaaaattct
    11281 cctgctgccg atactcctcc acctaacacg atacttgacg gatttaatgt attccccaag
    11341 tttccgcaag ctaaacctaa gtagaagcat acgcgatcaa caaccatcaa tgcaaacgga
    11401 tcgtcttctt gagccatttc aaagatatct ttacttgtta cttcttctcc gttgtctagc
    11461 attgctttca gttttgaatc ccctgcgtat tcttctgcca agaaacgacc taggcgtacc
    11521 acacctgtag cgctagatac agtttctaaa catccgcgtt taccgcaagt acattcgaac
    11581 ccttcaggat ccacagtgat atgtccgatt tctcctccag ctccgctcac gccgtgaatc
    11641 aaatttccgc cagcaacgat acctccgcca acacctgttc ctagagtaac gaatacaacg
    11701 tctggatcgt tttctccagc acctttccat cgttcaccta aagcagcaac attcgcgtca
    11761 ttatcgatag cgaatttgat tcctgttcct gcctcgatca gttttttcac aaattgaact
    11821 tctgtccagt ttaagttata cgcaccaata accgttccag cttcgctgtc cacgcttcct
    11881 ggggtaccca tgccgattcc gataaagtct tcggctttca tattatataa attcaaacga
    11941 tgattgattg attcaatgat ttcaggaacg atatgcttac catcatccaa aatattggta
    12001 tcgatgctcc atttttgctg aatctctcct tctggtgtta aaattgcaaa ttttgctgtt
    12061 gttccaccta aatcaatccc aataatcttc ttgtccatta caaatttcct ttctttcggc
    12121 tttcttcaat tcggtgctct ctttttaata tgcgccacgc tgtcatgtat gtatcatgat
    12181 caatcaaacg gctatcatac agattacgca attcgatagt cattaattca atgtcataga
    12241 tgcgtgcacc cacatagata aagatgccaa attttttgaa cagctgctgt acatcatata
    12301 agctctccat ctttaagcac ttccttcata ttatacaagt aaaccatatt tttttaaacc
    12361 cagtaaaata catattacaa aaagaaatac aaaaactagt gtagaaagaa tcctttggtg
    12421 tatcgaatag cttgtctgtt gtttcggtag acccaaaaca ttccctaaca ctaatccgcc
    12481 aattagccca ccgacatgtc cccaaacatc tacagaggta tccagaagac caaaaacaaa
    12541 tgtcaacaca ataaataaaa gaaaatgtct gactagctgc tggatcgctg ggttatgttt
    12601 aaaatgaaac cctaagataa acaacgcacc gaacaaaccg aatatcgaag tacttgcacc
    12661 accagacaga acattcgctt cgttaaaagc aaagcttgct gtatttccca ttatcccgct
    12721 gaacatataa atcaagaaaa aacgccaatg gccatagatt gcctcgattt gctgccccat
    12781 aaaatacagt atcagtgagt tcattacgaa atgcatcagt ccaaaatgga tgaagattgg
    12841 tgtcactaaa cgccaccatt cattgaagtg aacaagataa ggaccataca ttccggcata
    12901 ataagggatc tcaagtcgcg gaaataattc catcatgagg aagaccgctg tctgtatccc
    12961 taaaaaagca taggtccata acggtcgtct taaccatgca cgcaatttta tttgctgttg
    13021 ataattcatg aaaacctccc atttcatttt agtcaataaa caattgttgg atcttctgat
    13081 caaagctctc cggttgccat tgttcattca attgttcacg aaaaacaagg ctacatgttt
    13141 ttttatgaaa attatttaaa taccggtcgt aataaccacc gccaaaaccg attcgataac
    13201 cggctctatt gtagataata cctggtacaa tgaggagatc gatcatctcc ggcaaataga
    13261 gcttctctga ttgtggctca agaacaccaa aactcgaacg tacaagaacg gactgttcat
    13321 ccatttcata aaacgccatc tgacgtttcg gcaaagtttt aggtacaact attttctttt
    13381 gttctttttc tgctcgttgg ataaccggct gtgtatccag ttcaaattca ttcgataaag
    13441 tcgtccctat cacttttgct gacttccagt actcgctggc gaatagctgt ttatagattc
    13501 gctgttcttt ttcctttttc ttgccacttt tggccagatt tttcagcttt tcgattcctt
    13561 tccggcgtaa tcttactttc aattggtcca aaaactcacc cttccttctc tcataaaatg
    13621 gtataggaaa aaaagaaaaa tagcaatcga actttcaaat aaatgcataa atagaataaa
    13681 aagaagacct agaaatgatt ggccttcctt ttttaatgaa tttcaactgt tcagcgcgtg
    13741 tttttgtctg ataaaaagtg catacagaaa atatggcgca ccaaaaaggg caatcaaaat
    13801 tcctgtaggt acttccccac ttggtagaat cactctagca accatatctg cactaagcag
    13861 aaaaatggaa ccgaacaaac cgctcagaac aaaggtccag ttattttctt tacgtacgac
    13921 tagcttcgca gcatgaggag ctagtagtcc tacaaaagat aaactacctg tcaccgctac
    13981 actaccgctt gccaaagcaa cagctaacaa caaaaggatt ttttgacttt tagccacgtt
    14041 aaggcctaat ccttgcgcgc gttcttgacc aaatgatagc aatacaatcc tctgtgcata
    14101 aagtcctcct agcggaatta agataaccgc ccatggaagc aatgcccaaa cataaggcca
    14161 gctcgcaccc cagattgatc ccgctaacca tgcattcaca aatccataat tttccttaga
    14221 taattggatc gtgcatagca atgttaatgc agaaaggccg gcattaacgg caataccgga
    14281 caataagatt cgattcatac ccaaaaagcc atttcgatca taggccgcaa gatagacaca
    14341 gcaagcagct aaaacaccac ctagaaaggc tacaactagc aatagaaacg gcagcgaata
    14401 gtgcgaaaag aaacctaaat aaagcatcac taaaaatcct gctccggcat taatgcccaa
    14461 tatactagaa tctgccaatt catttcttgt tactccttgc aagatataac cagaaacgcc
    14521 aagacaagct cctgctaata aactgatcag taatcgagga actcgaaatt ctctaacaat
    14581 cagcgccaaa gaagatgatt ctttccctat gaacgtggca aaaagctgat ccaggtaaat
    14641 aaaaggcgta ccaatcatta aactacctat agacaggatg acaagtacaa aaagcaagaa
    14701 atagaacgtc caatatattt ttttcaccgt tgttccctcc ttacttgtat cagtaaaaac
    14761 ggaacgccaa tcaatgcaat gactaatcca aaaggtgttt caaaaggggg attgatcatc
    14821 ctcgctgtaa aatccgccaa cataaccaat ccaccgccaa ctaaaaaaga gacaggtaga
    14881 acataacgat agtccgttcc aaccattccg cgaacaactg gcggaaccaa cagcccaata
    14941 aatgaaacag gtccgactaa cgaaacggca atggctgcta acactgcaac tagacagaaa
    15001 acaatcaacc gcgttcgttt aggatgactt ccaagagcta atgcatgtgt ttcactcaaa
    15061 tgaatcaaag atattgcttt tcctaatgaa aaagaaacca aaacagcaag tatatataaa
    15121 ggaaacaata ctgctaattg tttccatgtg atattggcag ctccgcctac aaaccaaaaa
    15181 gcaagatcct gctgcagatt aaagaattgg gtaagtgctt ggctgatagc agtaaaaaaa
    15241 gaactaatag ctacgccagc taaaatcagc tgtaaaggat tcatgcccat tttcgaaaac
    15301 tgcaaaaaac caaatacgag caaagttaca gcaaaagaac ctgccaatga cattagaaac
    15361 acagaaaaat cagaagcttt aggccaaaaa gcaaaactaa gggccattgc caacgaagca
    15421 cccgcgttga ttcctagtaa gcctgaatct gctaaaggat ttcttgtcac tccttgcatc
    15481 aatgcaccgc tcaatgcaaa acaactccct acaagaaatg ctcccgccac acgaggcaaa
    15541 cgaatatttc gtatgatttg gtgactttgt tttgatctat caaaatcaaa caacgcctgc
    15601 caaaaatctg ctttttccac agtcgctgcg ccatttgaaa gagcaacaaa aaaaataatc
    15661 aaaagaacaa aacagaccaa aagcaggagt ctatttttct tcatagtcct cataattgat
    15721 cctcctgctc ttttggcaac aaatcatagg ttagaaaaac tggtttattt gttcttggaa
    15781 gcgtgacaat cgttgcatca atctcaaata tttttttcat acactctttt gtgatcactt
    15841 cttcaggtgt accttctgtg atcaacttac cgtttttcat tgctaataaa taatctgaaa
    15901 aacgtgaagc atgattaatg tcatgaatcg acatcaagat cgttgtttgt ttcatttgat
    15961 tgatttcagt caataactgt aggatctcta attggtgtgc cggatctaaa aatgttgtcg
    16021 gttcatccaa gatcaagata tctgtttcct gagcaagcgc catagcgatc cagacacgct
    16081 gggtttgtcc accagacagc gttgcgatat cccgcatacg aaatccagct agacctgtag
    16141 catccaacgc ccactcaatt gcttcatgat cttgcgtgtc catgccactg aacagttttt
    16201 gataagggta tcgtccgtaa gatactactt cttctactgt catacctgta gggtgttcac
    16261 tagtttgtgc taataatgca attttttttg caagttcttt tgaactgtat gtagagatat
    16321 cagagttatt gacaatgatc tttcctgatt tcacaggtag tatacgggta aaacttttca
    16381 ataaagtaga ttttccactt ccatttggcc caatcaaact agtgattttc ccttcaggaa
    16441 tcgtaacaga catgttttca atgatttttt tgttttcgta gccggctgca atgtcagctg
    16501 ctcttaatct cgtcatttca cccatccttt cgctgaaaat cattctcaat tatgacgctg
    16561 tttcaacatt tcgtcaagcg ctactttcat atctatagcc aaatgaagcg gttgacagcg
    16621 ttcgaaatat caggaataat aaccaatgag aaagattctc aattatgtta ggaggaaaga
    16681 aatgaaatta aaaacaaaag tattcacatt atctagcata ttgctgctgg ctggagtact
    16741 gagcgcttgc tccccctctt cagataaaga aacagctaca actgccaatt cgacgacttc
    16801 atccaatagt actcatacag tcacagatac acttggacat aaagtagaga ttccaaacgc
    16861 gcccaaacgg atcatcggca gttacttaga agactatctg attgcactag gggaaaaacc
    16921 tatcgcccag tggactgttg gcagtggttc cattcaagat tatctgcaag atgatttaaa
    16981 ggatgtccca acgatttctt atgaccttcc ttatgagaaa gtgttaagct ttgagcctga
    17041 tttgctgttg atttcttctt ccgctactgt cgaaggcggg aaatacgaac aatatagcaa
    17101 aatcgcgcca acttatgttg taaaaaacgg aacagatgtc acatgggaag aacaactaaa
    17161 agatgtcggc aaagctttgg caaaagaaaa agaggcagaa aaaatcattt ccgattatca
    17221 aaaatccatc aagaaaactc gagaagaact agcagataaa atcgagaaca agacagctgc
    17281 agtgttgtgg gtgaccaaca actcagcctt tatggtcagc aaaaatcggt caagtggaag
    17341 aatcgtatac gacgatctgg aatttggtgt tcctgatcta gtggaagagg tctccaaaaa
    17401 agccacttct gactggtctg ctgtttcctc tgaaaagcta gctgaattag atgctgacta
    17461 catcatcctt gtgaacagtg acaaagatgc tgccatgttc aatgaaccaa gctggaagaa
    17521 tctaaaagca gtgacagaca atcatgtact gcaattcgga cctgaatcta gttggctata
    17581 taacggacct attgccagtc aggaaatggt caatgatatc aaagaaaaac taaattgatt
    17641 ttaactaaaa aaagcatctt cacgattagt cgtgagatgc tttttttctt attaacaaac
    17701 tagacgcata gattttattc ttcttttgaa gcaaccctat tttttactct ttcccatttt
    17761 tgaaaacgat gggcatatag attttcttga tccgttatcc cctcatactc ctctataagg
    17821 gcgaagtctg aaaaatcaat attcccaata tacgtatcgc cttcaaacgt gctatcgatc
    17881 aatgttcgcc agatccgacc agtgtaagga agaagttctt gaaaaatccg actaccaccc
    17941 gaaacataga tatcttctga gatcgtttga ctatacgaca aaagttcatc gatcgaatgc
    18001 attacttccg cattttcttt cgtgcgaaaa tttggttgcg ttgtcaacac gatgatatgg
    18061 cgataaggaa gtgaaagatc ccccattcct tcataggtct ttcttcccat cactaaaatt
    18121 cgatccattg tatgttcgcg aaagaaacgc atatcatttg gcaatcgcca tggtaacaaa
    18181 ccatttttgc cgatcagacc atttttatct tgtgcccaca tggaaataaa catattatcc
    18241 gctccttatg attttagatg ttttgagata aaaaaagtct caattctttg accagcaagg
    18301 aattgagaac tttaaaaaca ttattttgtt tcacggtgca aagtaacttt gcgttcgcgt
    18361 gggcaatatt tttgtttttc caaacgatca ggattgttac gtttgttttt gcttgttaga
    18421 tagttacgtt ctttacaaga agtacactct aaagtgatgt ttacgcgcat gtttttccct
    18481 cccattttta caagaatcct ctaagatgac tctcagactt gattatctta ccatgatttt
    18541 ttctagattg ctagtgtttt tcacgatttt gttaagtaaa actacttgac gataaataca
    18601 acggttttac ttctcaaaca aaatcttttt ctttttgtat aaataccctt acacttgttt
    18661 atatagatta tgttttttaa taaaggggga tactaatatg ccatggaaca tgaaggactt
    18721 tcctgcatca atgaaaaact tagataaatt gacaagaaaa aaagcaatcg acatcgcaaa
    18781 tgcattatta gatgaagggt accctgataa ccgtgcgatc ccaattgcca ttgatcaagc
    18841 aaaaaaatgg tatgaagatg ctagtgaatc agaaaaacga actttcgaaa aagaaaaaaa
    18901 tccaagcaag acggatgaac acgatacgaa tcctcgtgct ggaaaactgc ttgattcaga
    18961 tgtgatcgta gaatatgagg aagaacaatg gatcgtgaaa tctaaagggg caaaaaaggc
    19021 aagcaaccat tttgatacaa aaaaagaagc aatcgaaaaa ggaaagcaag tagcacaaaa
    19081 caaagaatca actttgttca tttataaaaa agatgggaca aaagaaaaag aaatcgatta
    19141 ttgaacatat gtaaaggggt tgtgacgaaa atcgtgtcac aaccccctta tgttcgaaac
    19201 aatggtgttc ctctgccaac tccgaaccat aagtcataat atctataaaa cgggaagagc
    19261 tgttttttga aattgtttct tatcactcag tgttaaacgg taggagaaaa cctctttttt
    19321 attgatattt gattttcatc aaacagtcac atagtcgatt gcttctgctt gcggaatcgt
    19381 ttcatctaca aagtagactt tataccatgt caagaaactg aagattgtcc agatttttct
    19441 ttgctcttct ttacgtcctt cgtgatggtc atctagcaac ttcatgattt tttcttgatc
    19501 aaaaaattct tttgcaaaat ctgcttcgaa taatgctttg acttgttcgt agccgtgttc
    19561 ttctttcaac caagctttga ttggcacagg gaatcctaat ttcacacgat tcgaccattc
    19621 ttccggaaga tgtttgttcg cagcttgtct gaaaatatct tttgtgttgt tttgattcaa
    19681 taaaaactta cttggtattt gttgagccaa tttcatgact tcgatatcca ataaaggtac
    19741 tcggacttcc aaagaacttg ccatagaaag tttatctgcc tttaacaaga tatcttttgg
    19801 catccattga tgaagatcga catattgcat cttattgact tcatctttga ttccttctgt
    19861 tttctcgtaa tgaacagtca tgatttcatc cacagatggt gctgtttgat attttggtgt
    19921 taacacttct gatgcttctg attcttcgaa cactcgagca tgaccgataa aatattcttt
    19981 tgcaggagca agtgattcat ataaatggat ccgtccatgg aagtttttca tttttccgat
    20041 tttacgtcct aaattatagc gtgttccttt tggtaatttt ttcaatcctt gagcaatcac
    20101 acggatgaat tttgaattcg tatggaatcc atacgtttgg tatcccgcaa acagttcatc
    20161 cgccccttct ccagattgga ctacccgcac actttgtgaa gcaagatttg ctaaaaagta
    20221 caaaggtacg caagaaggat ttgaatcagg ttcatctaag tgatactgga tcaaagggaa
    20281 tgctttgaac gacatgtctc cgtcgatcac tgctgctgta ttatctaagt ccaatagttc
    20341 ggttagtttt ctggcttcaa tcgcttcatt ataggtttta tcatcaaacc cgattgagaa
    20401 agaatgatct ggtttcaata cagaggttac ataactggaa tcaaccccgc tggataagaa
    20461 agaaccaact tctacatcac tgaccgtatg tgcttcaata gaagcttgaa cggtttcgtc
    20521 gattttttcg atccattctt gtcgggaatg cgtttctttt gtttcaaaat ctgcatccca
    20581 atattgctga atgtctattt ttccatcttt atacgtataa taatggcctt caggcaaacg
    20641 atagacatct ttgaagaatg tttcgccgtt taatggagaa tattggaaag tcatataagg
    20701 tttcaacgct tctttgttca attctttgtt aaaatcagga tgaggcagaa aacttttgat
    20761 ttctgaacca aacataaatg ttccgttcat ttctgcataa taatatggtt tgatcccaaa
    20821 gtggtctctt gcaccgaata actcattctt ttcattatcc caaatagcaa aagcaaacat
    20881 tccgcggatc ttttgcaata attctgttcc ccattcttcg taaccatgca aaaggacttc
    20941 tgtatctgca tgtgtttgaa aaacgtggcc agctgcaatc aattcttctc tcaaaggctg
    21001 gtaattatag atttcgccat taaaaataat gatctttgtc cgatcttcat tgtaaatagg
    21061 ctgagtaccg ccctctaagt caataatact taatcttctg aatcctaaag caacatgttt
    21121 atcgatatat tctcctgaac tattaggtcc acggtgaacg attcgatcca tcattgtatt
    21181 aataattgtt tttttgttgt ccttgtcgtt aacaaaacct acaataccgc acatttaact
    21241 ttcacatcct aatataaaat tactgtttat ttcattcata tgcatcttat catagaataa
    21301 atagcggacg aatattttct ctcttacaat ttcgtcacgt gaaaacaaaa gatgaaagtt
    21361 tctgttttta cagaaaataa agtcccaata tctaatttac catgttttct tttttgaggc
    21421 taccttattc tcttcgtgtt aaaaaaatat tagtggatgc tatttccaaa aaaaccagca
    21481 aaaaaagaca cgacctaaag atcgtgtc
//
LOCUS       DAZUJO010000045        21457 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000045 DAZUJO010000000
VERSION     DAZUJO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.046"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACG8OL_001754"
     CDS             5..403
                     /locus_tag="ACG8OL_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4817741.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACG8OL_001755"
     CDS             387..1082
                     /locus_tag="ACG8OL_001755"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS4817742.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="ACG8OL_001756"
     CDS             complement(1676..2563)
                     /locus_tag="ACG8OL_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS4817743.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLIDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="ACG8OL_001757"
     CDS             2748..3587
                     /locus_tag="ACG8OL_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS4817744.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="ACG8OL_001758"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="ACG8OL_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS4817745.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="ACG8OL_001759"
     CDS             5055..5411
                     /locus_tag="ACG8OL_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817746.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5456..6571
                     /locus_tag="ACG8OL_001760"
     CDS             5456..6571
                     /locus_tag="ACG8OL_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817747.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="ACG8OL_001761"
     CDS             complement(6597..7037)
                     /locus_tag="ACG8OL_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4817748.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="ACG8OL_001762"
     CDS             7197..7703
                     /locus_tag="ACG8OL_001762"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS4817749.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="ACG8OL_001763"
     CDS             7871..9784
                     /locus_tag="ACG8OL_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4817750.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="ACG8OL_001764"
     CDS             9789..10790
                     /locus_tag="ACG8OL_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4817751.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="ACG8OL_001765"
     CDS             complement(10992..11198)
                     /locus_tag="ACG8OL_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS4817752.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="ACG8OL_001766"
     CDS             complement(11844..12692)
                     /locus_tag="ACG8OL_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4817753.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12856..13758
                     /gene="gnd"
                     /locus_tag="ACG8OL_001767"
     CDS             12856..13758
                     /gene="gnd"
                     /locus_tag="ACG8OL_001767"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS4817754.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="ACG8OL_001768"
     CDS             13760..14761
                     /locus_tag="ACG8OL_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS4817755.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFNVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMCEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="ACG8OL_001769"
     CDS             14758..15042
                     /locus_tag="ACG8OL_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS4817756.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="ACG8OL_001770"
     CDS             15075..15872
                     /locus_tag="ACG8OL_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4817757.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLSSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="ACG8OL_001771"
     CDS             15886..16719
                     /locus_tag="ACG8OL_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4817758.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="ACG8OL_001772"
     CDS             16737..17594
                     /locus_tag="ACG8OL_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4817759.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="ACG8OL_001773"
     CDS             17616..18125
                     /locus_tag="ACG8OL_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4817760.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="ACG8OL_001774"
     CDS             18162..18563
                     /locus_tag="ACG8OL_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817761.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="ACG8OL_001775"
     CDS             18675..19028
                     /locus_tag="ACG8OL_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817762.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="ACG8OL_001776"
     CDS             19062..19505
                     /locus_tag="ACG8OL_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4817763.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="ACG8OL_001777"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="ACG8OL_001777"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4817764.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="ACG8OL_001778"
     CDS             20600..21313
                     /locus_tag="ACG8OL_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4817765.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7387 a         3292 c         4474 g         6304 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tattaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 taatgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatgtg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgtcatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgtt
//
LOCUS       DAZUJO010000046        21302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000046 DAZUJO010000000
VERSION     DAZUJO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.047"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8OL_001779"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8OL_001779"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS4817766.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8OL_001780"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8OL_001780"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS4817767.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8OL_001781"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8OL_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS4817768.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8OL_001782"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8OL_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS4817769.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8OL_001783"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8OL_001783"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS4817770.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8OL_001784"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8OL_001784"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS4817771.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8OL_001785"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8OL_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS4817772.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8OL_001786"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8OL_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS4817773.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8OL_001787"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8OL_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS4817774.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8OL_001788"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8OL_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4817775.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8OL_001789"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8OL_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS4817776.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8OL_001790"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8OL_001790"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS4817777.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8OL_001791"
     CDS             15402..16376
                     /locus_tag="ACG8OL_001791"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS4817778.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8OL_001792"
     CDS             16546..17946
                     /locus_tag="ACG8OL_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS4817779.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8OL_001793"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8OL_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4817780.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8OL_001794"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8OL_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS4817781.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8OL_001795"
     CDS             19454..21253
                     /locus_tag="ACG8OL_001795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS4817782.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLFQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5925 a         4668 c         3840 g         6869 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttttca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ct
//
LOCUS       DAZUJO010000047        20630 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000047 DAZUJO010000000
VERSION     DAZUJO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.048"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACG8OL_001796"
     CDS             133..636
                     /locus_tag="ACG8OL_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4817783.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACG8OL_001797"
     CDS             763..1800
                     /locus_tag="ACG8OL_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFS4817784.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACG8OL_001798"
     CDS             1815..3512
                     /locus_tag="ACG8OL_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFS4817785.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACG8OL_001799"
     CDS             3505..3966
                     /locus_tag="ACG8OL_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFS4817786.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACG8OL_001800"
     CDS             3979..4839
                     /locus_tag="ACG8OL_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817787.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACG8OL_001801"
     CDS             4877..5266
                     /locus_tag="ACG8OL_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4817788.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACG8OL_001802"
     CDS             5253..7700
                     /locus_tag="ACG8OL_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4817789.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACG8OL_001803"
     CDS             7705..9813
                     /locus_tag="ACG8OL_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS4817790.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACG8OL_001804"
     CDS             9810..10814
                     /locus_tag="ACG8OL_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS4817791.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="ACG8OL_001805"
     CDS             10833..11744
                     /locus_tag="ACG8OL_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4817792.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="ACG8OL_001806"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="ACG8OL_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="ACG8OL_001807"
     CDS             complement(12406..12621)
                     /locus_tag="ACG8OL_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817793.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13089)
                     /locus_tag="ACG8OL_001808"
                     /pseudo
     CDS             complement(12880..13089)
                     /locus_tag="ACG8OL_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13392..14831)
                     /locus_tag="ACG8OL_001809"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="ACG8OL_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="ACG8OL_001810"
     CDS             complement(15379..16050)
                     /locus_tag="ACG8OL_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS4817794.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="ACG8OL_001811"
     CDS             complement(16399..17850)
                     /locus_tag="ACG8OL_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4817795.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(17962..19305)
                     /locus_tag="ACG8OL_001812"
     CDS             complement(17962..19305)
                     /locus_tag="ACG8OL_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4817796.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(19439..>20630)
                     /locus_tag="ACG8OL_001813"
     CDS             complement(19439..>20630)
                     /locus_tag="ACG8OL_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS4817797.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTPDGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDN
                     RSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIV
                     VVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQ
                     YHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDN
                     KTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRK
                     KENE"
BASE COUNT         6605 a         3529 c         3825 g         6671 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttagtaacg attatgtttc aatgtttaat cctgttactg cctttaaaat
    18001 aaaattatag ggttggctaa aactaggatg aaataaataa tctaacattt tcaatttgtc
    18061 taatgtaatt tgttcttcta tagcaagaga aaacattgat aatagtccag ataaatcttc
    18121 ttgactacaa agttgtacac ctactattct ctttgtattt ttttcaaagc taattcttaa
    18181 ttttacacga ctattttttc tcaaaaaaac aggtctaatg aaatcttcta tttctatata
    18241 atctatagac tcacttaata atcgtgctgt ttttaaattc aaacctgctg cgtaaaaatt
    18301 gtaattaaat atagaaaagc catttgtaaa ttgggaacca gaaggtgtaa aatcttgatt
    18361 gtcaatatgt tgagttgcaa tataagctgt tcttagagca ttagaaatag aaaaatcaac
    18421 actacttcta ttaatggacg taaaaaaagt tgtagcacaa tccccaatag catatatatc
    18481 ttttatattt gtttgctggt attcattagt tagatatgct ccactaacat ctctcaagag
    18541 ttcctttttg ccaattggac tattagatag aaatcctata gcttgtacga ccatatccat
    18601 agagtattgg gtaccacttg tagtgactat tttttcaatc ttttttcttc ctaaatattt
    18661 tttaggactt tctgatagac aaagatgtat tccatttttt gatagtattt cttcaacttt
    18721 atctgaaaaa ctacggtcgt aataagtcga caacactcta tctgcaacat cgaaaagata
    18781 tacttctttc cctctttttc ttatggcttc agcaatttca actccaatat atcctgctcc
    18841 aataattcct acttttttaa tagtattatc atctaaagct ttaaaaatct ctttagcatt
    18901 tggaaaattc tttatcgaaa aaatattagc taaattattc cccggtacat ctaacataat
    18961 ttgctttgaa cctgtagcta gtataagttt gtcatattct tgggaaaatc ttgtgccatc
    19021 tttttttaca caaataatct ttttattttg aatatcaatt ttgtcaactg ctgtctccaa
    19081 aaacaacttt tttatcttag aatttagttc tcttctattt tgataaaaaa gatcttctaa
    19141 attgctaact tctcttttta tgaacaacga tgtcccacat tctaagtaac taatttctga
    19201 ttttttatct atcagaataa cttcataatt tgaatcacat aagtttagaa atcgagctgc
    19261 agctagcccc gaatggtttg atccaataat tactattttt ttcacttaat cacattcttt
    19321 tcccttctat gaaaagtatc ttcattttta actaacaacc tatctcccaa aataaaaaac
    19381 aaaaagagga agaaaaagat tcttcctctt ttcagagaag atttaagaaa ttgattttct
    19441 attcattctc tttttttcga tttttatacc ctaataaccc taaagttcct aacaggatta
    19501 aaccaccact aatagtaaag atattactat caatctctcc cgtcttagga taagcttttg
    19561 aggactgctt ttgaggactg cttttgcctc tatttttgtt ttgcttgcta ccttgattgt
    19621 cagtcttatt atctttacta acggcttttt cactgtcttt ttcgtttgaa gaaacaacta
    19681 cattagtagg ttcactagta ttaccagatt catcagttgc agttactatc acttttgttc
    19741 ctgcctctaa tttaggaact ttaactgtaa aattaccatt tttatcagct gtcccatggt
    19801 attgactccc gtttggtaat gtaactgtca cctctgcatt tggttcagtt ttaccagtaa
    19861 caatttggtc accctctgta ataggatcaa cttgaggttt attcggtgct tttttatctt
    19921 ctacagaaat agtaacctct acttcttctt tagatccatc tggatatgtt actactacaa
    19981 tccctttttt gtttccagct gtactaacat ctactttctc tttccaagta taagtagttc
    20041 cttttggtag atcttctttg ttcttaattc cctcagatgc gtctggctct ttatttaagt
    20101 ctgtagtcac tttttgtcct tcaggattgt atttatcggc atcggaacga ttgtctgtca
    20161 cttctactgg aactttcact gtatctttcg taccatctgg atacgttact tcgataaccc
    20221 cttcgtaatt acctggtgtg ttggtatcaa tcgtaccatc aggagtgaca tctgttactg
    20281 ttgttccttc tggtaatgtt ggcaagttag taacattatc tgttagatct actgtgcctc
    20341 caacttctac tttttcgcct tctactgttg gctcatattt atcggcgtca gaacgattgt
    20401 ctgtcactgt aacatctact tcaacttcat cagatgatcc atctggatat gtcactacaa
    20461 cagtcccttt tttatttcca gctgcgctaa catctacttt ttctttccaa gtatacttag
    20521 tatcttttgg taaatcttct ttgttcttaa ttccctcaga tgcgtctggt tccttattca
    20581 attctgtagt cactttttga ccttcaggat tatatttgtc agcgtcagat
//
LOCUS       DAZUJO010000048        20395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000048 DAZUJO010000000
VERSION     DAZUJO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.049"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(383..1849)
                     /locus_tag="ACG8OL_001814"
     CDS             complement(383..1849)
                     /locus_tag="ACG8OL_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS4817798.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(2140..4224)
                     /locus_tag="ACG8OL_001815"
     CDS             complement(2140..4224)
                     /locus_tag="ACG8OL_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4817799.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(4749..5108)
                     /locus_tag="ACG8OL_001816"
     CDS             complement(4749..5108)
                     /locus_tag="ACG8OL_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS4817800.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(5138..5479)
                     /locus_tag="ACG8OL_001817"
     CDS             complement(5138..5479)
                     /locus_tag="ACG8OL_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817801.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(5476..6150)
                     /locus_tag="ACG8OL_001818"
     CDS             complement(5476..6150)
                     /locus_tag="ACG8OL_001818"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS4817802.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACG8OL_001819"
     CDS             complement(7326..8624)
                     /gene="asnS"
                     /locus_tag="ACG8OL_001819"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS4817803.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(8664..9854)
                     /locus_tag="ACG8OL_001820"
     CDS             complement(8664..9854)
                     /locus_tag="ACG8OL_001820"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4817804.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(9875..10402)
                     /locus_tag="ACG8OL_001821"
     CDS             complement(9875..10402)
                     /locus_tag="ACG8OL_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS4817805.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(10449..13238)
                     /locus_tag="ACG8OL_001822"
     CDS             complement(10449..13238)
                     /locus_tag="ACG8OL_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS4817806.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(13385..13585)
                     /locus_tag="ACG8OL_001823"
     CDS             complement(13385..13585)
                     /locus_tag="ACG8OL_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4817807.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(14038..14358)
                     /locus_tag="ACG8OL_001824"
     CDS             complement(14038..14358)
                     /locus_tag="ACG8OL_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817808.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(14861..15979)
                     /locus_tag="ACG8OL_001825"
     CDS             complement(14861..15979)
                     /locus_tag="ACG8OL_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817809.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            16212..16334
                     /locus_tag="ACG8OL_001826"
     CDS             16212..16334
                     /locus_tag="ACG8OL_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817810.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            16450..17757
                     /locus_tag="ACG8OL_001827"
     CDS             16450..17757
                     /locus_tag="ACG8OL_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS4817811.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            17878..19077
                     /locus_tag="ACG8OL_001828"
     CDS             17878..19077
                     /locus_tag="ACG8OL_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS4817812.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(19104..20372)
                     /locus_tag="ACG8OL_001829"
     CDS             complement(19104..20372)
                     /locus_tag="ACG8OL_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS4817813.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVRKGILFLPIVKRMKEKNKV"
BASE COUNT         5902 a         4344 c         3559 g         6590 t
ORIGIN      
        1 aatggtgttc aatcagctga ccttcagcat tagctcaaaa agcggcaaaa tatgaggagc
       61 tatttttccg tttgtttctc taatactcag gtcaaatcgc tgattttaca acctcttcct
      121 taaacggtgt tccaaagctt gcatctgcgg taataagccc agccaaacga aaaatatgag
      181 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
      241 gattcacggt gttccagaag tagcttcttc gaaaaacaag ccgaaacttc acaaaaattt
      301 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
      361 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
      421 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacgattg
      481 gtgtgatgat attcgtaaat aattggaacc accatgcttt aggatcagca gtataatcta
      541 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
      601 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
      661 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
      721 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aaaatcggca
      781 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
      841 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
      901 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
      961 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     1021 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcactagca aacagcatac
     1081 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     1141 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     1201 cccctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     1261 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     1321 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     1381 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     1441 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     1501 catttccttg gactgcccaa ccaccagcaa tcagaattgc ttgaggcttt tgtgccaaat
     1561 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     1621 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gatcctaatt
     1681 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     1741 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     1801 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     1861 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     1921 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     1981 ccatcaaaaa aataagttat tttcttttat ttcattgacc ttattgaatt tcaagctatt
     2041 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     2101 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct ctttttttac
     2161 atgcaagctt cgcatggcat tcaaaacagc taatactgtc accccaacat ctgcaaatac
     2221 tgcatctccc attgatgcaa aaccaaaagc acctaaggca agtaccaaaa tttttatccc
     2281 aatagcaaag acaatgtttt gtttgacgat tttcaatgtt ttctttgcta aatggattgc
     2341 ttctgcaatc ttcgatggtt gatcattcat gatgactaca tctgctgctt caattgcagc
     2401 atcgcttccc aatccaccca ttgcgattcc tacatctgct cttgctagca ccggtgcatc
     2461 gttgattcca tctccgacaa atgcggtctt tttattgttt ctttgtaaga tttcctctaa
     2521 ccgctgcact ttatcttcag gaagcaattc gctatatatc ttgtccacgc ctacttgctt
     2581 cccgatatga tcagcaattt ttttcgagtc gcccgtcaac atgactgtgt tcttcacacc
     2641 agcctgtttt aattgaacca atgcgtctac ggcatcttct ttcaaggtat ctgcaataac
     2701 aagataacca ctgtaacttt gatcgatcgc tagataaagc aatgtaccga tttcttgact
     2761 taaaggaaaa gagatttcaa atctttccat caatttgtga ttacctgcta atacttgttt
     2821 gccatcaact tcagctaaga tcccttcacc tgcaaattcc tggatattag ttgttggtaa
     2881 aagcggacct gcgtagcctt ctaaaacaga ttgagcaatc ggatgtgtag aaaactgttc
     2941 tgcactcgca atatattgca tgaatttctt tggatcgatt tttgtatcga tcgtctggat
     3001 cgaaaagtca cctttcgtta atgtgccggt tttatcgaat accactgtct cggtttgcgc
     3061 aagcaactcc aagtaatttg aaccttttac taatacaccg attttacttg cgcctccgat
     3121 accgccaaag aaactaagtg gcacggaaat cactaatgca catggacatg agatcactaa
     3181 aaatgtcaat gctcgataga tccattcaga aaacgccgct ccagaaacaa caagtggagg
     3241 taaaacagct aataaaacgg ccaatcctac aacgactggt gtgtaatagc gcgcgaattt
     3301 agtaatgaaa ttttcagctg gtgctttttt actgctggca ttttctacta attctaataa
     3361 tttgctgata gtagaatttt cataagattt ttctacttga actaaaaatg gtttattttt
     3421 attgataaat ccgcttaata ctgtttcacc aacatataca gatttaggga ctgattctcc
     3481 tgtaagtgct gacgtatcga ctaatgattc tccttcgatg atcgttccat ctaacggtac
     3541 acgttcacca ggcttcacaa gtacaaactg accgatctgc acgtcttcag ggttcacggt
     3601 catcaatcct tccgtcgtct gcacgtgagc tgtttctgga cggatcgcca tcaattcgcg
     3661 aatcgatttt cttgaatgac tgactgccaa tccttggaaa tactcgccga tttgataaaa
     3721 tagcatcact gctacggctt ctggatattc accgatcaac atagcgccaa tcgtagcaac
     3781 agacataaga aaattctcat caaagatttt tcctttgacg atattcatca ctgccttctt
     3841 cactacatca tatcctatca gcagataaat caataaatac cctatcaagc gaattggttc
     3901 attaaaatca aataatagca ataaagcgat acctgcaagg gaaaataaaa tccggaacag
     3961 cgtatttctc tctttttcat ttccatgttc agatactttt tccttatcta tttctgttac
     4021 tttgacatct ggttctaatt ccttcactac tttttttgtt tcttgttcaa tcgtagccaa
     4081 atgatcttcc tctgcgtcaa tcgttagttt tccagaagtg aagtttaccg ttgcttcctt
     4141 gactccattg atcttctgaa ctcctttttc gattttcatt gcacagttcg cacaatctaa
     4201 accttctaat ctgtaacttt tcattttaat gacctcctcg cctcaacata tgaacatctc
     4261 ttcataacta attataaatg aatggttatt catatgtcaa ggtatccgaa agaaaaaatg
     4321 aaaaacatct gcatcggaaa atttcttcca tacagatgtt ttggcttatt ctttcttttt
     4381 ctcttcgatc attgcccctg ctcttatcca acccgcactt gccttcttga tcttgacagg
     4441 atagcaacta agtttgaagc ctgttgctgg caatttgtct aaattcatta atttttcgat
     4501 aggacaatat cttttttcaa tccctgcaaa atgtccttcc cagatattcg aagctccact
     4561 acgctttcaa aaatatagaa agagttcttt aatgaaaatt atttcaactt tccataaaca
     4621 gtaatcccat ataaataaaa gacgtaaaag tcacatggga gaacttatac aggaaaacac
     4681 atagagtcag gtgaaataca tgtatcccac ctgactctat gcgttttcta acctttatta
     4741 ttctcttttt atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa
     4801 gattgcttgg caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca
     4861 tgtgcgagag caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac
     4921 atgccagact ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc
     4981 ttaaaacatt gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct
     5041 gctaaccagc taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa
     5101 caactcattg tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt
     5161 ttattcttct ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt
     5221 ccgtattttc ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca
     5281 cccgttcttc tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg
     5341 ttacagcaac gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta
     5401 cgaatgattc tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg
     5461 cccaccgtat ttccatcatt tctcactttt ctttcgctca tttttatctc cactaaaaaa
     5521 atcgactgcg gcaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt
     5581 tgggtggacc ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt
     5641 gaatatgcca tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat
     5701 ttggatttta gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac
     5761 aaacggctcc atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt
     5821 tcgataaacg gattcaaatt gctgcgcaga tttctcagtt ccgaatatag cctgatcgct
     5881 agtattatgc caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc
     5941 taaacaatct tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg
     6001 gttcatgcat tcaaatttca tcgttggata ttgggagatt agttttccgg caatcaaaag
     6061 tggataacct ttacctagat cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct
     6121 gtcaccgata aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga
     6181 tgattttctt ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta
     6241 taaaaaatga caccagccta gtcccttcaa tcaattgaat gaacgctcat caagtgtcta
     6301 gttttttata ctttttttct taacgtgccg gattcccctt gattccttcg ttgtttggtc
     6361 cagagaaatc gatcacacct aagcttccgt caatcagacc actgatgacg ccatcagaac
     6421 atatatttat aggctcatct aagacttcca gtaaatgttc ttttactaat atctcttcta
     6481 gattgcttgg gatagattgg tcataacttt atctggtccg atgatttcaa ggatgaatta
     6541 aacttacata tggatcattg catgctgaaa atacggacct actccatctt cgaatgttac
     6601 tattagtttg ttttcaggta ctagatgctg ctttatttca acagcagaag catcatcaaa
     6661 tcagatttcc atttgaataa ccttctttgt atatgtttga ttgattgcga tttcgttata
     6721 gaaccttgtg aatcaaatag caatttcttt tgctttcttc tcttgtctgc tggaaacttc
     6781 ctttatcccg tattttaaaa aaagaactgc gagagttcgc agttcttttt tttaaataaa
     6841 cgttgctctc cggccaactc ctcggtcaca agtcacaata gtcatcaaac atgaaaaact
     6901 gttttttgaa attactcctt gtgactcgga gctgaacggc aggcgcacaa cctcttgata
     6961 taaacggtgt tcaatcagct gaccctcgat attagctaaa aaaacggtaa aatatgagaa
     7021 gctatttttc cgttttgttt ctctaatact caggtcaagg cactgatttc acaacctctt
     7081 ccttaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     7141 gaaagccatt tccacaaaat ttttcttaat actcgaaact aaacagcttt ttcacaacct
     7201 ctccactaaa cggtattcaa aagcttacat ctgcggtaat aagtcccgcc aattcaaaaa
     7261 catgaacagc tgtttttgct tggctgttct aattacttga tgcagaacag ctttttcatc
     7321 acctcttatg gataaattcg gttcaacaaa cgtgggaatg gaatagcttc acgtacatgg
     7381 tcgataccag caatccaagt gactgttcgt tcaaggccta gaccaaaacc agagtgcgga
     7441 acagaaccgt atttacgtag atcaagatac catgaatatt ctttttcatc caaaccgtga
     7501 ttacggattt gttctagcaa gaaatcatag tctgttgccc gttcgcttcc gccaataatt
     7561 tcaccgtaac cttctggtgc gatcatatct gcacaaatta ccacgtcttc tcttgttgga
     7621 tgtggtttca tgtagaatgg cttgatggct tttggatagt ttaggataaa gacaggctgt
     7681 tcgaatgaat tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc ccattcgatg
     7741 tcatcaaatc catttttctt cagtaattcg attgcatcat cgtaagtaat acgagggaat
     7801 ggcaattttg tataagtttt taagacttct ttgtctcgtc ctagtacatc taatgcatat
     7861 tcgcaatgat ctaaaacatt ttgtactaag aatgctacat attgttcctg tacttctaaa
     7921 ctttcttctt gatgagtgaa agccatttct ggttcgatca tccagaattc gatcaaatga
     7981 cgtcgtgttt ttgatttttc agcacggaaa gtcggaccga atgaaaagac ttttccgaaa
     8041 gccatagcag cagcttccat gtataattgg ccactttgag ataaataagc tttttgatca
     8101 aagtaatttg tttcgaatag atccgtagtg ccttctggtg cagaaccagt caagatcggc
     8161 ggatcgattt tgacaaaatg atgttgatta aagaattcat acgttgcacg aatgatttca
     8221 tttctgatct gcataatcgc atgttgtctt gaagaacgca gccataaatg acgatgatcc
     8281 atcaagaaat ctgttccgtg ttcttttggt gtgatcggat aatcgtggct ctcgcttacc
     8341 acttccacat ttgtaattcc gatttcataa ccaaattttg aacgactgtc ttcacggatt
     8401 tctccagtta ctaacacgga tgtttcttgg tgtagatttt tagctgtttg gaaaacttct
     8461 tccgacactt cgctttttac aactacacct tggaaaaagg ctgttccgtc tcttaattgt
     8521 aaaaatgcga tttttccgct tgatcgctta tttgcgaccc aagcgccgat tgttactgtt
     8581 tctcccacat ggtttttcga atcgatgatt tgaatttgtt ccacgtatga ctctcctttt
     8641 atattccttg aatttcttca ggattaattt tgacttttac tctcaataaa ttgcttgatt
     8701 ctgcgtaccg cttcttctaa cgtagcgcga tcagtggcat aactcatgcg gacattttct
     8761 ggtgccccaa agccttcgcc agtcacaagc gctacccctg tttcttctaa aagatcttct
     8821 acccattgtg tgacattttc atacttacac atgcgtagcg tttcttttac attaggaaaa
     8881 agatagaaag ctccttgcgg cttttcaaga gaaacacccg gcagttctgc cacaagcgga
     8941 tataacgtat tcaggcgttc ttcaaaggcg ttccgcattt cttctactgt atcttgctca
     9001 cctttcaatg cttcaacagc tgcatattga ctgactgctg tcggattgct agttgattgg
     9061 gaagcaacag cgatcattcc attgatgatc acaggatttc ccaccgcata accaatgcgc
     9121 cagcctgtca tcgcatatgt tttagacacg ccgttgatga tgatggtctg atttttgatt
     9181 gcttctgaaa tcgttgcaat tggagtaaac tcattgccat tataaactaa acggccataa
     9241 atatcatcag caactatcaa aatatcattt tttactgccc attcaccgat tgcctgcagt
     9301 tcattcttgc tataaatcat tccagtcgga ttcgatggag aattgatgat tacggctttt
     9361 gttttttctg aacgtgcttg ttctagctgt tcaacagtaa ctttccagct attggattct
     9421 tcccctttca caaagacagg aagtccttct gccagtttta cttgctcacc gtaactgacc
     9481 cagtaaggca caggaatgat gacttcatct tgcgggtcaa ggatcgtttg gaaaagggta
     9541 tacaaagcaa atttcgctcc gtctgttacg actgtttgtg aaggttcata gacaagtcca
     9601 tagtttttct tcagataatc aacgattgcc tgtcgaagct ctgggattcc tgctgttggt
     9661 gtataatagc tagctttccc attgcggatt gcttcgattg ctgcttcttg gatgttttct
     9721 ggtgtcgcaa aatccggttc tcccacagtc aaactcagca catctctgcc ttttgctttt
     9781 aatgccttgg cctttgccgc agctgccaaa gtgacagacg gttctaatcg ttttgttctt
     9841 tttgataact ccatcccatt cattccttta ccttttaaat tcccttgatt gtcttgactt
     9901 catctccaga ttcaaagcca atcaggtaat aattcagttt cccatttgtt tcttttccta
     9961 ctacttccca cacaggctga tcatcataca tccctaaagc agctttttcg atttggattt
    10021 caggatgggc cgcagcaatt gtctgttttg cttcgttttc agtcagtccc tttgtttgat
    10081 ccaatacttt tactttttca ccagatttag gaatgataac ggcgatttct tttcctttat
    10141 catttgtacc tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa aaatgatcgg
    10201 tttcctcaag atttgcatat ttctttgcga tttctactgc ttctttcttc gcctgcaccg
    10261 ccggacgact ggaacgcaag taaaaaatcg agctgatcac gatgaccgct aataaaaata
    10321 cgatcaagcc aagtaacacg cgattaatcg ttttttcctt tgtctgtttt ttcttcaagg
    10381 gtcgtcgctc ctttatcttc attccttttc attatagcag attttaatta tgaaaaaaga
    10441 cagctcctct aacttccttc ttcatttttc tttaagaatt catggatatc ctgatagatt
    10501 tcctccatag ctagctcttt gatcggtact tttttcggta atgcatggat gatccgtgcg
    10561 ccatattttg cagtgactag tcttcgatcc aaaataagca atacgccacg atctgtctcc
    10621 gtccttatca agcgacctaa tgcttggcgt aacttcaaag ctgctttagg tacggattcc
    10681 tggtaaaaag gattgatccc ctctgattct aaataagcgt tcctagcttt gaccatcggg
    10741 cgaagcggat tatcgaaggg taaacgtgtg actacaacga tctgcaaagc atctccaggc
    10801 aaatctacac cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc tgacaaaatg
    10861 aagcgtttca aaagcttctc ccggcttcca ccaaaccctt gagctagtat ttctcgtcct
    10921 ttttctagca tagacagatg cattttctga tagaccttct gcaaaacatc atgagaagtg
    10981 aataacacaa gtattggccg atcttcttgt tcgatcagac ctgtaagtgc tgtctctaaa
    11041 taatcgatat atgcttgatt gctgacttga tggacggcag gtgcctcttt tggcacatag
    11101 atccttgcct gtttctcata atcataagga gaggcaatca ctttaagagg tgctgcttca
    11161 atgccaagcc tcttagagag atagtgtcga ttagggccga tcttcagcgt tcctccaata
    11221 taaagaatac gatcatatcg aggataccaa acagtcgacg gcaacatagc cgcttgaaaa
    11281 tcatggagat gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca atggatcaaa
    11341 tgatctcgcc agtcttccag ccattgatcc atcagcaagt attgcttgtc taattgatca
    11401 aataggctat ataaacttgc taaatcgatc cgcatctggt ttgtccagct ttcttggata
    11461 atctcaaaag tcagacgcat ttgtgactgt agctgctgga tctcctgata aaaaagctgc
    11521 aggtgatcaa tggctgtccc agattccagt ggcagctgat ccaacatctc ttttgtcatc
    11581 atgacttctt ctggatactg tccgttaggt tgcttgccgc tcactaaact cattaatcct
    11641 tctgccatac aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata aatcgataac
    11701 cagcgtaagg cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa cagctgattt
    11761 ggttcttcta attgctggat ttgtttatgg aaatagttaa tacttaatcg tttgtcattt
    11821 actttttcag cgatttccgg caaatgatgt acttcatcga tcaacaaaaa tggactagct
    11881 ggaagcaatg gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt gacaatcaaa
    11941 aaattacttt gcctgatttt cttttgcaag aaacgaacaa agtcttgttc gtaaaatgac
    12001 tgatttcttg ctaaaaatcc aatacctctg tgcgcgatat ctgcaaagaa tgaatggttc
    12061 aaatttgtta aatgtaattc atccaaatca ccagtcttcg tttgcgtcag ccaaactaag
    12121 accgccattt gatacaaagc atattgtttt tgttctggcg gttccacaag tgtgccttta
    12181 aaacgctgca aatcaatata gtggcgatag cttttgataa ttacaggttg tatgggctga
    12241 tctagcaatt gattcagcaa tggaatgtct ttttctatga tctgctgctg caaaactagc
    12301 gaaacggtac tgatgacagc gggtttttca ggtgtagcca aataactcaa tggcagcaga
    12361 taaccgattg ttttacccat tcctgtagct gcttcgatca tcaaatcttt tgattcatct
    12421 tttgtgaaat ggtcgtagac cgcattcatc agtctgttct gttcttttcg atacatcagt
    12481 gttttaccaa acattttttc ttttgcttgt ttttttcttg gatatgtccg ctcgccataa
    12541 tggacggatg tgaatagttc cacttctttt ttctgcaaag ctagcccatc gacgatttct
    12601 aatgagggat caagtggctc tggtttttct ttcatttctt caatcacatg ataaatgaaa
    12661 cgactcgtat ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa cgtgatgatt
    12721 ggcaattttc tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac ttcggcatca
    12781 ctatctgcct gatgaggatt ctcatgaata aatccaaggc tttctgacaa atcagctaag
    12841 cgaaagcttg gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc gatgcctggt
    12901 atcttcaaac taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt ataatcaaaa
    12961 taaatattat gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt ttgggcgaca
    13021 tcttcgaaat aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa atgctggatt
    13081 tgttttgaaa tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt gatccgacct
    13141 gattcaatca tgacgcagcc aaattggatg atccgatctt cttttggatt cgtcccagtc
    13201 gtttcgatat caacgactgc ataagttctt ggtttcaaaa tatcttcctc ttctcctgcc
    13261 tctaatgttc ttttgaaatt atactacaaa gtgcagaaaa atactctcat cgctttttaa
    13321 agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt ttagtatagg
    13381 ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct tcaacatcga
    13441 aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata gctgagaaat
    13501 gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt tctgagttaa
    13561 accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa cacatatttt
    13621 gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct accacagatt
    13681 accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct agagtttttc
    13741 gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa ccgaactttt
    13801 ttttaaaata aacgacatat tgttcagttg gtgttttttt gctctgatgt tcttttttaa
    13861 actaaaaaaa ttattctttc tgactactta tcggttctgt ttcaacggtc gaggctgggg
    13921 aagattcttt agtaggtctg atgttctcat ggtatttctg atagcctatg acagttacca
    13981 gaagaacaag gcaaaccaat acgaccaacg agattagttt ttcttttttg ctcatatcta
    14041 tacctcataa ataacaggat gatgtccatc atatttctga gtatgagtaa aagcaaaagc
    14101 tgcagctgta gaatgtaaga aatgccagca actttcataa tcacaggtcg gacagtacca
    14161 aaagtaacca aatcttggct gcaaagaccg ttcttctgtt tcaggaatcg cagaatcaag
    14221 atctggtgtc tgtacataaa catttttgta cagacgtgtg cctgtaatag gaaaccgctg
    14281 tgtttcttca gcaaaaactg gctgacttgt taaacttagg gatagagaca gcaaacacca
    14341 taacagtctt tttttcataa agacctcctt atcgataagt ttttcccaat taaaatcata
    14401 tctcaagaac gcccgttatt cacattttgt tcataaatga tgttttttgt atcataatcg
    14461 tcaaaaagag gtcgtgacca cgtcacaacc tcttttttaa acggtgttcc agaagtagct
    14521 tcttcggaaa taagccgaaa tttcacaaaa atttgagaaa taatttttgt caatctcttc
    14581 ttatttcttg aagctgacca cttctgtcac aacctcttcc ttaaacggtg ttcaaaagct
    14641 tgcatctgcg gtaataagcc caccaaacaa aaaatatgaa gagctatttt tgcttgctgt
    14701 tcttattact tgatgcagaa cagctttttc acaacctctc atacacggtg tttaatcagc
    14761 tgaccttcgg tattagctca aaaaacggca aaatatgtga aactattttt ccgtttgttt
    14821 ctctaatact caggtcaaaa cactgatttc acaacctctt ttaataacga agaatcgctg
    14881 tcagactctt ttcatctggc gcatcttttt gatccaaaat aaacactttt ccgccattaa
    14941 tgatcacatc atctgctaat gcattcaaca cttgacgccg atcatattct gtttcaggat
    15001 cttcaccgaa accatcagct aagtttgacg tagcgataaa taaatgtgac acttttcctt
    15061 ctttcgcagc tggaacgata tcaactaact gatcgatgaa tttcttatct aagagtttat
    15121 tgtattcttt tgattcttga gctgcgagtt cacttgacag cttcttcgcc ccttcttcga
    15181 tatctctaaa ggatagctgc gcaggagaag cagaaatcgc aacgtctgca caataatagg
    15241 gatttttagc aatttttttg aacatcgtct gattttctgg tagagcaaat aaataaatcg
    15301 gccattgttc tggattttcg aaagaatcct tcaagtacgt atcaactgct tggtaatagt
    15361 tgacccaatc gatctctact tcttcatctt ttggatttac gccatggaaa gttactccat
    15421 ctcgggctcc gcctcctcca ttagaagaga agttcaaacc gccacctgtc agttcagatc
    15481 ccaatgcagt aacgacatca accggtgccc cttctggtag agatacttct tttacttgtt
    15541 tgttgctgac ttcatacaat ttcatggagt cgcggttcaa tcccaataaa taataagaat
    15601 agttgaactg cccatttttg ataatagcca gtaaataagg aagatcactt acatagtatt
    15661 gatcgtctac cgggatactc aaacgatgga tataggtttg gtcagcagtt ataatgaccg
    15721 ctacactcgt agttgcacta cgccaaaacg aaggatctcc taacaaattt tcaatttttt
    15781 cttggaacgg ggaccagtca tgttcggaat attttttctc aaaacgtttc tttgcttctt
    15841 tcgcaaaatt tttgaatttg atctgatctt tttccacatc ttgatgggcg acatgcgtat
    15901 tcagcatgat cgtgacgaat ggtcctttag cgccttctga agtcaattgt gacaataatt
    15961 ctttatcctt ttttgccata aacgtacccc tcctaaaaaa atcattcctt tgtttctatc
    16021 taaagtctac cacaacacta tttttccata caataaaacg tatctaaaaa aataaaacgg
    16081 tttctacggt tttaaattta gctatttttt taagttatct tcataatttc ctcaattatt
    16141 tagccccagt cttcaactat tcttcatgaa atgctgataa actaagaaaa agaataaaag
    16201 aggtggatct tatgcatttg attatgattt tcagtttatt aagtattatc agtttatttg
    16261 ctttcagcgc ttcccgctta ttagatgcgg aaacacagcc tattcctgta aaagtacgta
    16321 aaaacgaccg ctaaccttcc ataacgtgcg ttcttatcct aaagtttttt cagaaacatt
    16381 gaaactctct cccctctctg ataaaatttt aatttgtagc gaaacaaatt aattacaagg
    16441 agatcggata tgtccataaa aaaaacacgt ctagtcctat tattggctag tctcattctt
    16501 tgccttgctt cttttctacc agctgttgct gtgcaagctg aagatacttt caaagtaaat
    16561 gcaaaagcag cctttgctgt agatgcgcaa agcggtaaga ttctttatga tcaagatgga
    16621 gaaaaaacca tggggatcgc ttcaatcaca aaaatcatcg gtctttacat cgtccttgat
    16681 caagtaaaag aagggaaact atcatgggat gataaagttt caatttcaga ttatgcagaa
    16741 aatttgagtg tcaccccaga tttatcaaat gttcctttgc ataaagaaaa cacctacacc
    16801 gttaaagaat tattcgactc tgcaatcatc cagtcagcca acgcttcaat ggttgccttg
    16861 gcagaaaaaa tctctggcag cgaagcaaaa ttcactgaac gaatgaaaga acaattgaaa
    16921 gattggggta tcaaagatgc aacgatcgtt aatgcatcag gactgaataa ctcttatctt
    16981 ggcgaaaacc gaccagaagg tactggagaa aacgacgaaa atcaaatgtc tgctcaagat
    17041 gtggctatcg ttgcccgtca tttgatcttg gattttccag aaatactgga tgtatctagc
    17101 actacgacac aaatgtttgg cgaaaacacg caatcaccag tagaaatggt caactggaac
    17161 tggatgctcc caggattcgt gaattataaa gaaggcgtgg acggattaaa aactggtacg
    17221 actgaacttg caggagcctg tttcgtcggt acgatcacta aaaacgatca acgaatcatc
    17281 actgtcgtat tgaatgcaga cggccatgcg gaaaacccaa gtgcccgttt caatgaaaca
    17341 gctcgcttaa tggattattg ctacgacaat tggtcagaaa aagagcttgg aaaagccaat
    17401 gcaagtatcc ctagtctaaa aaccattgat gtaaaagacg gaaaagaatc aacagtgccc
    17461 gttgtgctta aaagtcctgt aaaagtatgg gtacggaacg atatggatac tggaaaatta
    17521 acaatcacac caactcttga caaaaaacaa gtcactgaca atgagatcca agcacctatg
    17581 gataaaggaa caaaaatcgg taccgcaacg atcaccttgg cagaagataa attaggttat
    17641 cttgaagaca acaacgcgcc atccacagaa atcatcacgg ataaaaccgt agaaaaagca
    17701 aatattttcg ttattggctg gcgacgcgtc gcagaatttt tcggtaactt gttttaattg
    17761 aacaaaaaga aaaaagcatc tttccatgtg gaagatgttt ttttgctttt tctagtttaa
    17821 ataacagctt ttttcatcta ttttcgttat actgtataag aaaaaggagt tgaatttatg
    17881 aatctaacca gagatctgat ttttttggtt ctctatctta ttggggtatt ctccatcgtt
    17941 tattggcgaa aacagaaaaa ggatccttcc ttgttctttt acagcagtac atggacgttt
    18001 gcggtaatct tgacttattt tgtcgtgttg gaaatttttt attcgacctt ttattttttg
    18061 attcccttag tgtttttcag tatctttgcc ttttcctatt tttcagaaaa gcgaaggctg
    18121 ttgaatggtc ttttattcaa tattttcctc attagttttg gtatctactt gtttgtcttg
    18181 ctatacgaaa cacaaaatat ttttcttggc ggactgattg ccttgatcac gatcccgtta
    18241 ctgcttgtct ttctattcgg catttatgga ttgatcgttt ttcttttttg gaacggtgtc
    18301 acagttttac gtagagagtc gcattcatta gctaatcttt taacgttgat actagccatt
    18361 tttcttacat tgtttcttgt ttttgacttt ttcttgttaa aatatttacc acaatggata
    18421 aatgctttgt ttttctgcat cccattgatc ttgatctatc tatttatcgt attctacaac
    18481 tttttgactg tctcattctt gtatcagttc aaccgccctc gatacaacca agacttcatc
    18541 gttgtgttag gcgccgggtt gatcaatgga gaaacagttt ctcctcttct tgccaagcga
    18601 atcaataagg cgattgcttt ttattgggca caaagccgtg cgacattaaa tccgcctatc
    18661 ctattaatgt cgggtggaca gggagcagac gaaaaagtac cagaagcaat ggcgatgaaa
    18721 caatatgcca tggaacaagg aattcctgaa agagatattc ttgtcgaaac caattcgaca
    18781 accactttgg agaatatgcg ctattcaaag gaaatcatgg atcagcaaat gaaaggtccc
    18841 tatcgcgcga tcttcagttc caacaattac cacattttca gagctggtct ctatgcacga
    18901 caagcgaaac tgaaggcaaa tggcattggg gcaaaaacag ctttctatta cttgcctaac
    18961 gcgtttttaa gagaatacat cgccatcttg atgatgaaca aaaaaagaca tatgatcatt
    19021 tgcggattgc ttttcgcagg ttccgcattc ttatcactga tcactttact tttctaaaaa
    19081 aacaaggtgt gacaaaacat atgtcacacc ttgttttttt ctttcattct cttaactatt
    19141 ggcaaaaaga gtatcccttt tctgaccgct acttgctgag cagaataaat accgctgttg
    19201 cctgaacaca taaagctgat caagcagatc ataaagaagt atactgccgc ttgtgaaccg
    19261 aacaattcga tccccatcac aaagcaggca atcggtgtat ttgtcgcacc agaaaatact
    19321 ccgataaagc ccaatcctgc aagaaaagga acagaaacat ggagcaaagg cgctagtgct
    19381 gcaccaagcg ttgcaccaat ttcaaacaat ggtgtcactt ctcccccttg atatcccgcg
    19441 cctaaagaca aaactgtgaa gaataacttc ccaataaaat catagacatg tgcctgtcca
    19501 ttaaatgatt cctgcagtaa tggaagactc aatccgagat aacgttgtgt ttgcaaaatc
    19561 aatgctgcgg ctacaactac agccccacct atcgtatttc gccaaacagg atgaggaatc
    19621 cattgtgcat atcgcgcttt taaaaagacg atcgaacggc tgaataccca accaatcaaa
    19681 ccaaaagcaa tcccagcaac aaaaactttg aagaacagca acatactcca ttcaggaatc
    19741 acacccattt ggtaatgtag atgagaaaca ccatagcttt ctgtcataaa atttgcaaac
    19801 agtgcagcaa agaagctagg gaagatcgct tctgttcgga ctttcccgat tgccaacact
    19861 tctaatccaa ataatgttcc agctaaaggt gtgccaaata cagaactaaa acccgcactg
    19921 attccactga taatcaagat ttcccgttct gatttatcca atcggaacaa acgtgctgta
    19981 ttatctgcta acgctccccc catttggact gctgttcctt cacggccaac agatcctcca
    20041 aataaatggg tagttatggt tccaaacagt gtaagtggaa taagtcgcaa cgggattttt
    20101 tcttctccac catttccttg atcgatcact aagttgtttc cacgactaga aagaccgccg
    20161 tgatacgcat agagataagc aaaaagcgca ccactaactg gtagtagata aaggagccaa
    20221 ggatgtgata gtcggatatc tgtcacaact gtcaggctat ttaagaaaaa agcagataag
    20281 ctccccatga atataccgag aatcgctgtg atcaccagcc acttcagcat gtacactaaa
    20341 atgcgtactg gactcggtaa ttgtttttcc ataaaaaatt cctcctcaat aaaaa
//
LOCUS       DAZUJO010000049        20181 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000049 DAZUJO010000000
VERSION     DAZUJO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.050"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="ACG8OL_001830"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="ACG8OL_001830"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS4817814.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="ACG8OL_001831"
     CDS             1011..2258
                     /locus_tag="ACG8OL_001831"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS4817815.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="ACG8OL_001832"
     CDS             2334..2678
                     /locus_tag="ACG8OL_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817816.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="ACG8OL_001833"
     CDS             complement(2795..3121)
                     /locus_tag="ACG8OL_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817817.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="ACG8OL_001834"
     CDS             complement(3494..4696)
                     /locus_tag="ACG8OL_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4817818.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="ACG8OL_001835"
     CDS             4813..5328
                     /locus_tag="ACG8OL_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4817819.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="ACG8OL_001836"
     CDS             complement(5527..5973)
                     /locus_tag="ACG8OL_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4817820.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACG8OL_001837"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="ACG8OL_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4817821.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="ACG8OL_001838"
     CDS             complement(7852..8481)
                     /locus_tag="ACG8OL_001838"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4817822.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="ACG8OL_001839"
     CDS             complement(8465..8929)
                     /locus_tag="ACG8OL_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4817823.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="ACG8OL_001840"
     CDS             complement(9416..10684)
                     /locus_tag="ACG8OL_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS4817824.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="ACG8OL_001841"
     CDS             complement(10915..11652)
                     /locus_tag="ACG8OL_001841"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4817825.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="ACG8OL_001842"
     CDS             complement(11719..12288)
                     /locus_tag="ACG8OL_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS4817826.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="ACG8OL_001843"
     CDS             12416..13288
                     /locus_tag="ACG8OL_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4817827.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="ACG8OL_001844"
     CDS             13630..14502
                     /locus_tag="ACG8OL_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4817828.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /gene="nagE"
                     /locus_tag="ACG8OL_001845"
     CDS             14499..16457
                     /gene="nagE"
                     /locus_tag="ACG8OL_001845"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS4817829.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="ACG8OL_001846"
     CDS             complement(16708..17559)
                     /locus_tag="ACG8OL_001846"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4817830.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="ACG8OL_001847"
     CDS             17682..18149
                     /locus_tag="ACG8OL_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS4817831.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="ACG8OL_001848"
     CDS             complement(18412..18894)
                     /locus_tag="ACG8OL_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4817832.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="ACG8OL_001849"
     CDS             complement(18994..19857)
                     /locus_tag="ACG8OL_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS4817833.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6067 a         4156 c         3662 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgacta caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgactccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactaacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt t
//
LOCUS       DAZUJO010000050        19507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000050 DAZUJO010000000
VERSION     DAZUJO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.051"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8OL_001850"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8OL_001850"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS4817834.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG8OL_001851"
     CDS             complement(1173..1868)
                     /locus_tag="ACG8OL_001851"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS4817835.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG8OL_001852"
     CDS             complement(1881..2597)
                     /locus_tag="ACG8OL_001852"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS4817836.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG8OL_001853"
     CDS             2927..3532
                     /locus_tag="ACG8OL_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS4817837.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACG8OL_001854"
     CDS             3857..4180
                     /locus_tag="ACG8OL_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4817838.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACG8OL_001855"
     CDS             4362..5732
                     /locus_tag="ACG8OL_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS4817839.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACG8OL_001856"
     CDS             complement(5862..7163)
                     /locus_tag="ACG8OL_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4817840.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACG8OL_001857"
     CDS             complement(7393..9384)
                     /locus_tag="ACG8OL_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS4817841.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDKVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACG8OL_001858"
     CDS             complement(9528..9794)
                     /locus_tag="ACG8OL_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4817842.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8OL_001859"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8OL_001859"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS4817843.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8OL_001860"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8OL_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS4817844.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACG8OL_001861"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACG8OL_001861"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4817845.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACG8OL_001862"
     CDS             complement(13954..14475)
                     /locus_tag="ACG8OL_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS4817846.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACG8OL_001863"
     CDS             complement(14478..15320)
                     /locus_tag="ACG8OL_001863"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS4817847.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACG8OL_001864"
     CDS             complement(15313..16038)
                     /locus_tag="ACG8OL_001864"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS4817848.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACG8OL_001865"
     CDS             16214..16510
                     /locus_tag="ACG8OL_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817849.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACG8OL_001866"
     CDS             17078..18670
                     /locus_tag="ACG8OL_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4817850.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACG8OL_001867"
     CDS             18683..19423
                     /locus_tag="ACG8OL_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4817851.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4001 c         3702 g         6224 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt tatcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAZUJO010000051        19484 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000051 DAZUJO010000000
VERSION     DAZUJO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.052"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..3659
                     /locus_tag="ACG8OL_001868"
     CDS             132..3659
                     /locus_tag="ACG8OL_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS4817852.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="ACG8OL_001869"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="ACG8OL_001869"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS4817853.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="ACG8OL_001870"
     CDS             7447..7788
                     /locus_tag="ACG8OL_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS4817854.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="ACG8OL_001871"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="ACG8OL_001871"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS4817855.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /gene="pheT"
                     /locus_tag="ACG8OL_001872"
     CDS             9145..11565
                     /gene="pheT"
                     /locus_tag="ACG8OL_001872"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS4817856.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ACG8OL_001873"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="ACG8OL_001873"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS4817857.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="ACG8OL_001874"
     CDS             12999..13736
                     /locus_tag="ACG8OL_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4817858.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="ACG8OL_001875"
     CDS             13750..14565
                     /locus_tag="ACG8OL_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4817859.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="ACG8OL_001876"
     CDS             14578..15225
                     /locus_tag="ACG8OL_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4817860.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="ACG8OL_001877"
     CDS             15241..15897
                     /locus_tag="ACG8OL_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4817861.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="ACG8OL_001878"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="ACG8OL_001878"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS4817862.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="ACG8OL_001879"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="ACG8OL_001879"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS4817863.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="ACG8OL_001880"
     CDS             18239..18757
                     /locus_tag="ACG8OL_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4817864.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /gene="cbpB"
                     /locus_tag="ACG8OL_001881"
     CDS             18864..19364
                     /gene="cbpB"
                     /locus_tag="ACG8OL_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS4817865.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6554 a         3344 c         4183 g         5403 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagcgttt aactccaaga aataagaagg aatt
//
LOCUS       DAZUJO010000052        18564 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000052 DAZUJO010000000
VERSION     DAZUJO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.053"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8OL_001882"
     CDS             6..305
                     /locus_tag="ACG8OL_001882"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4817866.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8OL_001883"
     CDS             378..1739
                     /locus_tag="ACG8OL_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS4817867.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8OL_001884"
     CDS             1761..2021
                     /locus_tag="ACG8OL_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817868.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8OL_001885"
     CDS             2035..2787
                     /locus_tag="ACG8OL_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS4817869.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8OL_001886"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8OL_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4817870.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8OL_001887"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8OL_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS4817871.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8OL_001888"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8OL_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4817872.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8OL_001889"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8OL_001889"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4817873.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8OL_001890"
     CDS             7161..8558
                     /locus_tag="ACG8OL_001890"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4817874.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8OL_001891"
     CDS             8639..9079
                     /locus_tag="ACG8OL_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4817875.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8OL_001892"
     CDS             9227..9553
                     /locus_tag="ACG8OL_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4817876.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8OL_001893"
     CDS             9554..10303
                     /locus_tag="ACG8OL_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS4817877.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8OL_001894"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8OL_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS4817878.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8OL_001895"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8OL_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817879.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8OL_001896"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8OL_001896"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS4817880.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8OL_001897"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8OL_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4817881.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8OL_001898"
     CDS             14770..17436
                     /locus_tag="ACG8OL_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4817882.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8OL_001899"
     CDS             17800..18552
                     /locus_tag="ACG8OL_001899"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4817883.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
BASE COUNT         5964 a         3212 c         3832 g         5556 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct tttt
//
LOCUS       DAZUJO010000053        18198 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000053 DAZUJO010000000
VERSION     DAZUJO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.054"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..840)
                     /locus_tag="ACG8OL_001900"
     CDS             complement(16..840)
                     /locus_tag="ACG8OL_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS4817884.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(813..1493)
                     /locus_tag="ACG8OL_001901"
     CDS             complement(813..1493)
                     /locus_tag="ACG8OL_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4817885.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1516..2454)
                     /locus_tag="ACG8OL_001902"
     CDS             complement(1516..2454)
                     /locus_tag="ACG8OL_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4817886.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2699..3550)
                     /gene="ispE"
                     /locus_tag="ACG8OL_001903"
     CDS             complement(2699..3550)
                     /gene="ispE"
                     /locus_tag="ACG8OL_001903"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS4817887.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3704..6277)
                     /gene="mprF"
                     /locus_tag="ACG8OL_001904"
     CDS             complement(3704..6277)
                     /gene="mprF"
                     /locus_tag="ACG8OL_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS4817888.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6418..7224
                     /gene="proC"
                     /locus_tag="ACG8OL_001905"
     CDS             6418..7224
                     /gene="proC"
                     /locus_tag="ACG8OL_001905"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS4817889.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7303..8859)
                     /gene="rny"
                     /locus_tag="ACG8OL_001906"
     CDS             complement(7303..8859)
                     /gene="rny"
                     /locus_tag="ACG8OL_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS4817890.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9270..10319)
                     /gene="recA"
                     /locus_tag="ACG8OL_001907"
     CDS             complement(9270..10319)
                     /gene="recA"
                     /locus_tag="ACG8OL_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS4817891.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10428..11669)
                     /locus_tag="ACG8OL_001908"
     CDS             complement(10428..11669)
                     /locus_tag="ACG8OL_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS4817892.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11909..12487)
                     /gene="pgsA"
                     /locus_tag="ACG8OL_001909"
     CDS             complement(11909..12487)
                     /gene="pgsA"
                     /locus_tag="ACG8OL_001909"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS4817893.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12696..13619)
                     /locus_tag="ACG8OL_001910"
     CDS             complement(12696..13619)
                     /locus_tag="ACG8OL_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS4817894.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14357..15643)
                     /locus_tag="ACG8OL_001911"
     CDS             complement(14357..15643)
                     /locus_tag="ACG8OL_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4817895.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15636..16904)
                     /locus_tag="ACG8OL_001912"
     CDS             complement(15636..16904)
                     /locus_tag="ACG8OL_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS4817896.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17156..17617
                     /gene="mscL"
                     /locus_tag="ACG8OL_001913"
     CDS             17156..17617
                     /gene="mscL"
                     /locus_tag="ACG8OL_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS4817897.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17676..18122)
                     /locus_tag="ACG8OL_001914"
     CDS             complement(17676..18122)
                     /locus_tag="ACG8OL_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4817898.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         5190 a         3981 c         3181 g         5846 t
ORIGIN      
        1 tcttttcttt ataatttagt tattttttct tctgaaggca accaatattc gtttgatgat
       61 atttacaatc aggaacaaag caataaagac taatgtgatt gttgctcctg gaggtgtatc
      121 tagataaaac gagctagtta gtcctgacag catccctaga aatcctacac aaacactgat
      181 agcaatcacg acattgaagc ttttgcctaa tcgcataccg attgctgcgg gaagaaccat
      241 gattgcggat atcaaaagtg caccggcaat cgggatcatc acagcaatag ccacaccagt
      301 aatcacattg aacatcatcg acatcaaatg aacgggtagc ccatccacgt gggcggtatc
      361 ttcatcaaaa gtcagaacat acatcggccg tttgaataaa aagaacaata taccgatggc
      421 aacgaataag attcctaaaa agatgacttg ctcccatgta atggtcacga tcgaaccaaa
      481 cagataggat tggatacttg ttgctgaatt ccctccgctc aaattcatca atactaacgc
      541 tagcgccaaa ccaccagcca ttaaaatggc aatggagatt tctgaatagg ttcggtagat
      601 cgttcgcaga tactccaata tcacagctgc taatacaacg actagcatgg tcgtaagtgt
      661 aggattaagg ttcaaaaaga atcctaacgc tacccctgct aaagagacat gagaaagcgt
      721 atcagccatc agcgattgtc ttctgatcac taaaaacaca cctagcatcg gggcgattcc
      781 ggcaatgaag atagcggcaa gaaacgctct tctcatgaat tcatatgaaa gcaacgccat
      841 tgagaatcct cctttctcac cagtcggatc tgtctgtccg catactgttt gatatcttca
      901 tgatcatggg tgatcattag aactgatttc ccatggacat gcgcactatg tcggagaaga
      961 cgataaaatt catttctgga ttcttcatcc atacctgttg ttggttcatc aagaacaaat
     1021 aagtcagggt cagtagcaaa aatacgcgct aaactgattc gctgcttttg tccgccagac
     1081 aattcaccta ttcgccgatt tcgcatatcc cacatcccta cagcttctaa agcacgctgt
     1141 acatgttgat gatctttttc agagagacgc ttgaaccagc ggcctcttgg atatcttcct
     1201 gaacggacta attcgatcac cgtactagga aaacctgcat tgaaagatgc aacttgttga
     1261 ggaatataac cgatacttaa tttttttcct tcttgatttt tctcagcaat ctttatcatt
     1321 ccttttgaag gtttcagtaa tcctaacgat gctttcacca aggttgactt ggctgctcca
     1381 ttttctccag ttaaaatgac aaattcaccc gcatctacat gataagagac atcttctagt
     1441 acaggttcat catcatagta aaaggtcaaa tcagatattt cgatataacg caccttacaa
     1501 cctccattct aaaacttaat tgatactttc ttttaaagct ttcagatttt tttccatgac
     1561 agaaatataa ttttcgcctg ctttgatttg ttcattagtc aagctttcta aaggattcaa
     1621 cacttctagc ttcactcctg tttcattcga caatgtctca gctaccttcg aagaggcatt
     1681 ttcttcaaaa taaatcactt tgatgttgtt tttcttcaca aattctttta gctcagctaa
     1741 gcgacttggt gtgggttcct gatctggtga aacgcccgaa atcgctactt gttttaagtc
     1801 gtattgattc gctaaataag caaaagcagc atgctgagtg acaaatgtgc ggtttgttgc
     1861 atcttttagc tcatcggagt atttttgatc cagtgcttca agctttttga tataagcatc
     1921 ggcattcttc gtaaaagttt cctttttatc tggatatttt tcgcctaatt cttcagcaat
     1981 cgttctgact tcttttatag ctaataccgg atcgagccaa acatgtggat cgtattcatg
     2041 tgaatgacca tgcccttctt ctgattcttc atgttcgtct gcttcttgcg attctaaacg
     2101 gtcgattcct ttagctgctt cgatcatcaa ggtctgtttg ctatccactg aatcctttaa
     2161 cgaatccaca aaaaattcca tgtctgaact attatacacg aaaacatctg catcggaaat
     2221 ttctgctctt tcttttgcac ttggttcgaa atcatgcggc tccgttccag cagggatcaa
     2281 aagtttcacg tttccttcat cccctacgat ctcttttgta aaatcataca tcggataaaa
     2341 agtcgtaacg atcgtcaaat cctctttttt gtctgcttcc ttgttcgtat ttccacatgc
     2401 tgcaaacagt aaagcaccta tcattaccgt agctgtcaaa agatattttt tcatttatat
     2461 gctcctttca atgaacaaat cggaatttta cgatttatta acgtgaacta atgtatcaaa
     2521 ataaaaaaac acagtcaata gaaatccatc aatttctgat ttaagcatgt atattcttga
     2581 ttaaatcgta aaatggatga gttgctgctc atatctagcc atttcactac aataaaaaac
     2641 tgaagaatac cattttagga ttcctcagtt tttcatcacc tattttactt cttatatttt
     2701 attgcagggt tcgtactaaa taaacttcct cacaaaaacc ttttaaccca ttatatactc
     2761 tctgcgctct tgaataatgt tgacacaagg caaaaacggt tggtccgctt ccactcatca
     2821 atgcagcatc tgctccgtac ttgatcatac gctctttgat ttgatggatg actggatgcc
     2881 gctttcccgt tatatcttct aaactgtttc ccattttttt gatcatctgc tgataatctt
     2941 gctctcggat ggcttcagca agtccgtgga tgtcttgatg ttggatggct tgcgtatcta
     3001 cttctctaaa cactgtacgt gtagagacac tcattcttgg tttgaccaac acaacccagc
     3061 actgtggcat agaaggtaat acttggattt cttccccttt gccaccgaca aatgccgtac
     3121 ttccgtagac acagtaagga acatctgtac cgacttccaa accgatttct gccagttctt
     3181 ctaacgaaca gcccagattc cataattggt tgatccctct tagagcagct gcacagtctg
     3241 tacttcctcc gcctaatcca gctgctactg gaatattctt cttaacaaag atcttgacac
     3301 cggttttgat attgtattta ttttttagca ataaagcagc ttgataaaca ttgttccgtt
     3361 catctaccgg taaaaatgct ttatttgttt ctacaatgat tttgtcttct gttatttctt
     3421 caaaaaatag atgatctgcc aagtctacgc tcgacatcac catttccaac tcatgatacc
     3481 catcttgccg tctatataat acatccagtc ctaaatttat cttggctggc gctttttcaa
     3541 tcaattccat cctctcaccc attcgaaaag tctaagtcgc aagtattcta tcatagggaa
     3601 aaataaaagt atagcttatg tctgcctagt ggttgatttt gtcatcgttt caacagcttt
     3661 cacagaattt tctcttcata atccaaacaa gttctagtga ttcttataat tctgtgagca
     3721 cttcttctgc caaactcgga acatttcctt tttcttttcc tatcagactg agtagctgca
     3781 tggtagcaaa tccgaaatta cttcctttcc tataagcaaa ataacgtcct tcccaatgat
     3841 cgacatattt taatttcttt tcataagcag actggaagga atagaaaaaa ttaccataat
     3901 tatatacgat atgcatgact ttttcttgaa agaaagaaaa atctgtttct ccaacattag
     3961 ctaatggagc taagcctaga ttggcttccg agtagcccat tttctcgtat acaataaaca
     4021 gattggctaa cagatagtca gctaattcct cagattcttt aggtaagacc cgcaacaaat
     4081 catatcccgc ctgcttcttc gtcatcggct gagcggtgat gaagccaacg actttttgat
     4141 ctttacggac aataccgaca ccgctagata atacatatga tttatcaaat cgtcccacag
     4201 agaagtattt ttctcgtgca ccttgaagcc atttttgtga aacttcattt acagcttgat
     4261 gtaattcttc cggcagttga tcgtaccaag taaaatggta tccttctttc atcaaacgtt
     4321 gatattcaag ttgctgactc agccgagaca agtcttctgt tgctagatca acaatccctg
     4381 cttcacctac tttcgtgaag tgaaaaccca attcatgaag catcactacg tagctttctg
     4441 atatcttata aaacgcaagc tgatagccta aacaatctgc ttgtttcata aactgattcg
     4501 ttgctgcccg catcatttta ggattgccaa ctggatctcc taaaacaaag catttgtttc
     4561 ctttgatctg ataaccaaaa acgacttgat cttggtcgtc ttcttggtaa taaaaataac
     4621 tgtaacctga caagcgaagc tgatggcttg tagctgtgcc tccgtatgtt tccaataaac
     4681 ggtcaaaacg gtctttttcg tactcactgc caagcttcgt agtatcttca gctaaatatt
     4741 gataaagcgc cactaaagct agcaatgaga tgcccaaacc aataagccct gagaaccaca
     4801 catcatctga aggaaacaaa ataaaatgat ttcctgttat tcgaccgact aaaaagcgtc
     4861 cgctgtgata gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag ctagcatcaa
     4921 agattatagc tccccaagaa taaacgaatt ttgttcgata aaattctttt ctggataacc
     4981 acacagctaa cagaatcaag acatatacca atatcaactg ccaagataac gtacgtgtga
     5041 ttgtatttaa aataccaaaa gatagtaaag caatcgtagg cagaaatgcc tttttgactt
     5101 ttactgaaag tgctctagca aggccgagta ataaaaagcc aaccaataga ttcactgtct
     5161 gatctaaaaa attgaatgag aacggcaata atacttgaaa tagccgactg attgcagaca
     5221 gattagtgat tgttgataga agtaccatca tgatccccgc aaaatagaca gcacttacga
     5281 gcacgatatg cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa aatcgattga
     5341 ttttgactcc tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt agaacataat
     5401 aaaacaaccg ataatataat agccaaacta ctgctacatc ttgccgaaca ggcaattgac
     5461 tcattcctaa gatcatcaaa acgtcaaaag tccccattcc acctgggacc atcgtcagca
     5521 ttcctatcaa tgtagcaatc atgaacatag gatagatcga aagcatcgaa atatctattc
     5581 tcatcaactt gccgatgatc aaaaataccg caagggctcc cgtccattgt ccaaaggatg
     5641 ccagtagcag accaagcaag ccttttggat aaaactcgaa gaacaatgtc tttctcttta
     5701 gataagcaaa aaagaaaatg actggtgcta aaaaactacc gccgaaaagc cagatccaat
     5761 agcttcgaaa aatactttct gactgaataa aaaagacatc aataaatgta agaaacgacc
     5821 atacagacaa accagtcagc ataaatagtg cgacttttga tacagtggct aaaaccattt
     5881 ttctatttgt ctgctttcca taaaaactag cccgcagact tgctcctacc acaccaccaa
     5941 aaccggcgag attgttgatc gtgtttgtta cccacgcagc aagaagaaac tcttttcttt
     6001 ccatttttgg ttttccttgt ttttctaaga ctctgatcgt cacccaatca tacaacaaca
     6061 tcggagtgac acccagcagt ccagccagaa tcatgagcag gatcgtcatg cggctttgtt
     6121 gtcccatcct ttgccaaata tcttgccaac tcatgccatt agcaatattt gcgacttgat
     6181 ttgccacaaa gactaacacg gagccgaaaa aaatcagttt taaaatcaga ctatgttcct
     6241 ttatccattg cagcatcaag tatcgtttat ttttcacatt ttcccattcc taacttgtat
     6301 acttttgtgt cataccccaa tgttatctta tttattttaa tttttccact ttagacctgt
     6361 tctttgcttg cgatgataga aaaagaaaga tatcctaatg ctaaaggaga ggataacatg
     6421 aaagtaggat ttatcggcac agggaatatg gcacaagcta ttattgatgg tttattaaaa
     6481 aaaggaacag tctcgaaaga agaggtgttc gtttccagtg gacattttga aaatgcacaa
     6541 aaatttgctg ctgaaactgg cgtccatgca tgcaaaagta atcaggaagt tgcatcacaa
     6601 tcagatatcg ttcttttagc cgtgaaacca aaaatcattg aaaaagtatt ggaagaacta
     6661 agagaatata gccagaaagt cttattcatt tctattgctt caggaaaatc attaacagat
     6721 ttagaaacag cgcttgaaga tcctcaggcc tccatcgtac gtgtgatgcc gaatgtcaat
     6781 gtcagcgtag gagcaggcgt ttctgcgatt tgcaaaaatg aagctgccac ggaggaacag
     6841 cttcagcaag ctacgatgat ttttgaagca atcggcagcg tctatccttt agcagaatca
     6901 gatttcagta cattcattgg ccttgctggc agctcacccg cattcgttta catgctgatc
     6961 gatgcgatgg gacggactgg tgtactgaat ggtttgtcca aacagacagc cactgagatt
     7021 gctgcaaaag caattatggg ttcttgtcaa aaattccttt caagtgataa aaatccatgg
     7081 gaacttgtag accaagtttc ttctcctggc ggtacaactg ttgcaggtgt agttgcctta
     7141 gaagaagcag gattcatccc agcaatcatc aaaggaatca atacgacgat cgagaaagat
     7201 cttgagatgt tgaataaaaa ataatgatag caatgttttt tcgtgcctta caaaaaaaga
     7261 agagctgtgg cttttgccgc ggctcttctt ttgtattttt tattatttag cataatcagt
     7321 tgcacgagtt tcccgaataa cgattacttt gatgtgtcca ggataatcca gttcttcttc
     7381 gattcgttta cgaatatcac gtactaaacg aacagcgtct aaatccgaga tttcttctgg
     7441 cttgaccatt acccgaactt cgcgtccagc ttgaactgcg taacttgatt ctacaccggc
     7501 gaagctgttc gaaatatttt ccagacttct taaacgacgg atgtagtttt ctaatgattc
     7561 acttcgcgca ccaggtcttg ctgctgagag cgcatctgct gcagctacaa ggacagagat
     7621 aacagaagta gcttccacat ctccatgatg agaagcgatc gcgttgatta ctactgggtt
     7681 ttctttgtac ttggcagcta attccgcacc gatctcaacg tgagatcctt caatctcatg
     7741 gtcaagtgct ttcccaatat catggagcaa cccggcacgt tttgctaatt ggatatcttc
     7801 gccaagctct gctgctaaga taccagctaa ttttgcaacc tcgaccgaat gcttcaagac
     7861 gttttgtcca taactagttc tgaatcgcag acgtccgatg atcttgatta gatctggatg
     7921 aagtgtatga gcaccgactt caaacgctgc ctcttccccg tattcgcgaa tacgttcatc
     7981 catttcttta cgagatttct cgaccatttc ttcgatacgc gctggatgga ttcgtccatc
     8041 ttgtatcagt ttttccaagg tcatccgtgc gatttctctt cggattggat cgaatccgga
     8101 aagaacaact gcttccggtg tatcatcgat gattaaatcg ataccagtca gcgtctcaag
     8161 cgttcgaata ttacgacctt ctcgtccaat gatacgacct ttcatttcat cattcggaag
     8221 ggtaacgact gaaacagtcg tttctgaaac ttgatctgct gcgcagcgtt gaatcgctaa
     8281 tgataataag tttttcgctt tgcgatctgc ttcttctttg gcacgctgtt cagattcttt
     8341 taccattaga gttaactcat gattcagctc ttcttcagtc gatttcatga tgacatctcg
     8401 tgcgtcctct tttgataaac cagcaatttt ttctagttct ttttgttgat cttcaatcat
     8461 tttttcaacg tctttttctc gctcttcaat taattgctgt tttgaactaa gtgcttcttc
     8521 tttactttca agtgagttct cgcgtttttc taacgagtca tctttacgat tcagcgtttg
     8581 ttcgcgttga atcaaacgat tctcttgaga ttttaattct aacttacttt ctctcagctc
     8641 actttcaact tcagaacggt atttctgatt ttcttcctta gcttctaaca atgcttcttt
     8701 ttttagagtt tctgcttctt tttttgcact atcgatgatt ccagcagctg atgattgtgc
     8761 accagctatt tctttctcgt gacgtgactt tgcgaccata aaccctaaac caagaccgac
     8821 aattaaaccg atgatagcga ggagaatgtt gaataccata aaatccacct ccatactatc
     8881 tttttcattt ttatcatcta tacagcttct atgataaatt attctaaact cgtcaatatg
     8941 catcttcgca tatatgtgta caaaaaacat gcacaacttt attctaatgt ttgtaccccg
     9001 tagtgtcaac tttaaataaa caaagaaacg gctttctact tattcattaa acgatggttt
     9061 ttaaaataaa tgacactagt ttcatatagg taaaccttta aattgagaaa cttttctatt
     9121 aaattattct ttgtagatga acagatattt ttattttatg tacccactcg tataattaaa
     9181 taactcacga tttctttatt caataaaaaa acggcaggtc aaggagagta cctactcttc
     9241 agaccagccg tttttgttta tcataatttt tattcttcta ctaaaggcaa ttcttcttgt
     9301 cctttttcat cttctgggac agcgacttct tcaccaatac cataagctgc tctaactaga
     9361 gcagaaactt ctgccatcat ttcaggatga ttagccatat agattttagc gttttcccgt
     9421 ccttgaccaa tccgatcttc tttataagaa taccaagcac cgcttttatc aacaatgtct
     9481 ttttctactg ccatgtctag aagttctcct tcttgtgaga tccctaaacc atacatgaca
     9541 tctacttcag cgattttgaa aggaggtgct actttgtttt ttacgacttt gatctttgta
     9601 cggttaccaa caatatctgt accttgtttc aattgttctg cacgacgtac ttccaaacgg
     9661 atcgttgcgt agaatttcaa tgcgcgtcct ccaggagtga tttctggatt cccaaacatc
     9721 acgcctactt tttcacggat ttggttgatg aaaatagcga ttgtttttgt tttgttgatc
     9781 gaaccagaga gtttacgcaa tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag
     9841 tcacccattt cgccgtcgat ttctgctcgt ggaactaaag cagcaactga atcgactact
     9901 acgatatcta cagccccact tgatactaaa gcatcagcga tctctagacc ttgttctcct
     9961 gtgtcaggct gtgaaagaag tagttcatcg atattcacac ctaatttttg tgcatattgc
    10021 ggatctaacg catgctcagc atcaatgaaa gcggccgttc cgccattttt ttgtacttct
    10081 gcaatagcgt gtagtgcaac tgttgtttta cctgaacttt caggtccata tacttcgatg
    10141 atacgtccac gggggtaacc gccgacacct aaagcgacat ctaacgcaag agagccactt
    10201 ggaattgtag agatttgttg gtcgattttt tcgcccaatt tcataataga gcctttacca
    10261 tagctctttt caattttttt cagtgcagca tctaaggctg ctttacgatc atctgccaat
    10321 acgataatcc tccttatatt tacaaatcag agcaacattt cattatcctt atcatatctt
    10381 ttttagtgaa aaaaagcaag aaaaaaccga acaaatattc gcatttatca ttttcttaaa
    10441 agctgttttc tcaaaagatc aaatccttgc ataaccgcat tatatctgat agcagcacga
    10501 tctctggcaa agtgatattt ttccgaaacc acgccgtctt tagatgaaat agcgatccat
    10561 actgttcctg cagattgacc ttctaacgtg tccggtccag ccactccggt gaaagctaca
    10621 ctgtaatctg ttcttgctat ctcacgtgct tgaatggcca tctgctccac acattctttg
    10681 ctgactgttc catattctgc taatagctcc ggatcaatct ccagaaaacc agcttttgtt
    10741 tggagcgagt aggtaacgaa tccgcctgga aatacttcag atacaccagc aatgtcgcct
    10801 aatgcggctt ggaaggcgcc ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt
    10861 tctttcagta gttccactac gacttttgct agcgaattat catcgccgta tccataaaaa
    10921 tattccccta ctcgttcttg gattttttct tctaatgcta aaagagcttg attccctgcc
    10981 tgaacatcat ttgttttcac tgtcagtcgt aatgtcactt catttggttt ggcatatggt
    11041 gcgatcgttg gattgatctg tgtctcaatc agatctttta gctcagtgac cagtcgcgat
    11101 tcaccaatcc cataaaagcg caaaacttta gaaattagtt tttcttcaga aggaaaacgt
    11161 tcctctagta aaggacgtac ttgttccaca aacattggtt tcaactcatt aggcggaccg
    11221 ggaagaagga tatacgcatg tgtatctgtt tgataaaaag tccctaaagc aagccctgta
    11281 cgatttggta gaggaacccc gccttcgata atctgggact gttgcagatt gtttttggtc
    11341 atttttcgat gggttgtttc aaaatatgcc aataattttt tgtatccttc cgtattttgt
    11401 atgagagact taccgaggtg agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt
    11461 cctaatccgc cacataagag tatgagttca cttcttgttt cagctagagc aatcaactct
    11521 tctaaacgct gctgattatc tcctacaact gtttgataat aaacttcgat acctagatca
    11581 gctagctgtt ctgacaagaa agtggcattc gtattcacga cttgtcctag taaaagttct
    11641 gttccgacag ctattatttc tgctttcatt acttcccgct cccttatata gtatacgctg
    11701 tatttttata tttttttatt taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca
    11761 gctccttgtt ccaaataaac agtgttctcc tgccaaaacc tcggtcataa gtcacaatat
    11821 acataaaaca tgaaaagctg ttttttgaaa ttatcccttg tgactcagag ttagacggca
    11881 ggctcacaac ctctccacta aacgatactt acattgatcc tttgaacaca ccgctgtttt
    11941 tgatgaagta atccacacct gaatagatag taaagatcag acaagcataa agcatgattt
    12001 gatctactgg aataccgatc aggctgaatg ggatattatt gataaacagc aagataatag
    12061 caaccatttg tgtagccgtc ttgacttttc ccggccaagc tgcagccatg acttctccat
    12121 gttcgacaag tagtaaacgc aagcctgtga cggctaattc acgacagaca atgatcgcta
    12181 ctacccaacc aggggccttt ccttggtcaa ccaacatgat aaaagcagtc ataactagca
    12241 ttttatctgc caacgggtcc gcaaacttgc caaaattagt gacaaggcct cgcgcacggg
    12301 caatttttcc atctaaccag tctgtgatac tagctaaagc aaaaataact gcacctacta
    12361 gctgtgtgac agccagtgtg gtgtctccta cgttaaattg tccccaatct aaaggtaaac
    12421 ctacaaccaa aatgaaaatc gggatcatac aaattctaag tacagttaat ttatttggta
    12481 aattcacact atcgactcct ttcttcccta ttctacgtga ctatcagcga agtttccacc
    12541 gatattattt acttttccaa atgaatttat tcaaaggagt atttttattg aaattgatct
    12601 ttttgaatta acgaagaagg accgcgacac agatttgtca cgatccttct tttttatacc
    12661 tacaagtaaa cagatgcgta aactaattta tacgatcatt ctgcgtattg aagagtaagg
    12721 ttcacatttt ttggactctg attgttttcc cctggattaa ctggtacgga ttctccattc
    12781 gctttgatcg ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc
    12841 ggtaacacgg tactctgtgt ttcatttgct gccagtgtcc cttgataaac ataggcattg
    12901 tctactagca cacctaacca tacacgattg acagcagtaa attcaagggt tacaggcttg
    12961 gtagcgtctg tgatatttac tgcgatggca ctgccagaat cttctcctgt agctattgcc
    13021 attttctttt cttcacttga ttttgttgtt gattctactg tagaagactc tttggtctgc
    13081 gtgctttgct ctgaggaaga ggactcctcg actgatcctt ctactttaag cgaagaagct
    13141 gtgtctccga ttattggatc agcttgacga tcttgccaag tcatatagcc cacgacaacg
    13201 acaatcgcca aagcaaccaa tcctaataaa atgacaggaa gacttgtcca aaagcgactt
    13261 ggatgcgatt cttcctgatg cattgctttt ctagaagtac ttactgtttc aggctgcgga
    13321 cgttcaggga ccgatcctgt caatttgctt ttcccatcaa aaacatcgac taaatggtct
    13381 ccatcttctc ctacagcttc agcgtattgc cgaataaatg cccgcacgta aaatttacca
    13441 ggcaattggt caaaatcatc attgtcgatt gcttccaaat aacgtttttg aatttttgta
    13501 atctgctgta attcatctaa agatattttt ttattcaggc gtgcttgccg taatttttct
    13561 ccgatggttt caaaagccac tcgttcatct tctccagtct gtttgattct ccgaagcatc
    13621 tgctgttgca acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc
    13681 ttttttccga ataaacattg ttctcctgcc aactcctcgg tcacaagtca caatatccaa
    13741 taaatataag aagctgtttt ttgaacttgt tccttgtgac tcggagttaa acggcaggct
    13801 cacgacctct ttcttaaacg gtgttcaatc agctgttcct cggtattagc tcaaaaaccg
    13861 gcaaaatata aggagatatt tttccgtatg tttctctaat actcaggtca aaacgctgat
    13921 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc
    13981 aagttaaaaa cattaagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
    14041 gctttttcac aacctcttgg tacacggtgt tccagaagta gcttcttcgg caataagccg
    14101 aaatatcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
    14161 ccacttctgt cacaacctct ttgattactt cgtttgattt tattttaata taagttgatt
    14221 aaaagagagc atgatttgtt ttattttctc actaattttt ccaacacaac taagaatacc
    14281 ataaaagctg attttggagg aggttcctac tacaagtcta aaacgaaatt taagactttt
    14341 cctcataaat actggtttat tttggtctca tgtaaaactc gctgaaggcc tcttcactta
    14401 taaatgcttc cccagctgcc agtacgtctt tcatttggat cgaatcgatg atttcaggaa
    14461 gatcaaataa agttcgatcg ccaaataaac tttgcgtaaa ttgattggca atatattcga
    14521 tcgaatttaa tgattggaaa tattgtccaa gcattttctt cttcagcaaa tcgagatttg
    14581 tttctgacac ttccggatca tcagcgaatc caagaatgat ttctttgact ttttctgctg
    14641 ctttctctgg ttcgtccgta tctccgctga agtccgcaaa gtgaaattca cgatccaagc
    14701 tgaattcaaa accaaagctg tcatcgataa ttccttgatt gtacatcgct agatagtttc
    14761 gagaagtatt gcctaaaatc atttggaaca acagattgat tgctgtttta tagcgtaaca
    14821 gttctgttcc ttcttgcggc aatgtgtcta atcctttaat accaagtaca aatttatctc
    14881 gtgtaatggc tgcttccaat gcactttgtt tgatgatttc ttttgtgttt tcagggaaat
    14941 aacgtacaat ttcttgcttt ggagggaaat tttttgcttc ttggttttcc cgaataagtt
    15001 tcatcaattt ctcaggctcc atcttaccaa ctacaaataa aaccatattg cttggctgat
    15061 aaaacgtccg atagcacgta tacaagtctt gagctgtgat tttgtcgata ctttcaaccg
    15121 tgcccgcaat atcgatatgc aatgggtgtg taggatacaa gttatttagt atgccaaaaa
    15181 acatccgcca gttagggtcg tcttcataca tttggatttc ctgaccgatg attccttttt
    15241 ccttgtttac agtttcttct gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca
    15301 gattcttttc tacctgatcc gtcgtagaaa acagatagct ggttttcgta aaacttgtga
    15361 aagcattggc ggaagctcct tgtttcccaa ataattgaaa gacatcacca tcttcttttt
    15421 caaacaactt atgttctaaa aaatgggcaa ttccatctgg aactttcacc ttttcttttt
    15481 caccataggg aataaattcg ttatcgatcg aaccataatt cgtactgaac aaaccatatg
    15541 tcttgtgata gtcattttta ggcaacaaat acacggtcaa accgtttggc agtacttcat
    15601 gataaagtgt ttcattgatt tgctcatatt ctgttttatt catttgcatt tcctcctgct
    15661 aaaaagaaga ttgcctgcaa ttcgatttgc tcagctactt gttgtatctc tttgatcgtt
    15721 acgctctcca tacgtttcaa ccattcttca tcagataatt ttgtttccgg cagccagctg
    15781 tccaaaaaag aagcttcgat tgccgcttgc ggagagtcta gagaaagcag atattggttt
    15841 ctcagcattg cttttgtctg agacagttcc aactctgtta tttcaccatt tcgcaaactt
    15901 tctaattgct cgtgaatcag atgaagtact tgatttcggt tcttttcatc gataccggtt
    15961 tggacactca tgaatcctcg gaacgtatcc acgctactag aggcataata agctagactt
    16021 tctttctctc tgacattcat gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta
    16081 aagaccatta gcgcaaaacg ttctggttca tcgtaataca catttgtctg ataagctagg
    16141 ttcagctttg cttgaacgat tggctcacgt acttgtcgtt ccttcacgat attcacttga
    16201 ggttgggtat aaaacagatc tggataaact gtttcccgcg actcaaatgg taattgccca
    16261 actgcttcag ctgctttgtc cggatcaaca tcgccgacaa caaaaatgtc gatttgatca
    16321 tttgccatca tttcttgata gtaggacgct agcgaactag cagtgatctt gtccaaatct
    16381 tcgactacgc ccaaccctgg gatcttttgt tccgggctgt tttcaaaata aagttcttgc
    16441 aaggcatagg aagcaaaagt ttgtttatct tccttcaaac tttccaaata tagacgcaga
    16501 ttgttctttt ccaagtcaaa cgtcaactga tcaaactgat gatttttaat atttggataa
    16561 aataaaacct cttttaaaaa atctaccgct tgagaaaaaa ggtttggatc attgacatat
    16621 tcaccattca caaaactgat ccctgcgttt agccaatgta aatttccttt ttttctaact
    16681 gataatccaa agcttgcgcc ataaagctct gcaagttgcg cacttaactt tgtctgatcc
    16741 ggataattca aactatttgt ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact
    16801 tcttgttgca gacgcgcagt aaaacggata aaaatccgga tcgttttgta ttgttttgtt
    16861 ggtaagacat gaagattcac gccttctgcc aattgataca ccatatttat atgctccttt
    16921 tcacagttga ttgcccgctt actcttttaa tgggtcaacc tgcctttttt acttattcct
    16981 ctgcttgaaa tttataagtt caggcaattc gccgtttttt ctaaatgatg ttacacttta
    17041 tagtatagca tagatgtttt ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt
    17101 tttatttttt atttttcgat atactaaaga caaataaaag aagggagggt cattcatgat
    17161 caaagaattc aaagagttca tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt
    17221 tattggtagt gcgtttacaa gcatcgtaaa ccaagtggtg caaggcttga tcacaccact
    17281 aataggttgg tttgtcgcaa tgatcacagg tacttctgat ttagaaggag cactttcaat
    17341 tcttgactgg tcgccgacaa aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat
    17401 cattacattt ctgatcactg gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc
    17461 gaagaattta cgctccaaag aagagcaagt ggaagtggaa gaaactccta ctgcagaaga
    17521 ttatcttagt gacatacgcg atcttcttgc caaacaagtg gcagaagaca atacacatcc
    17581 agtagagcaa acaaatcttt ccgatacttc taaataataa agaatctaaa aaaaagaaag
    17641 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    17701 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    17761 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    17821 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    17881 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    17941 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttgtccatca
    18001 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    18061 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    18121 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    18181 aacgaacctt acgaatct
//
LOCUS       DAZUJO010000054        17984 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000054 DAZUJO010000000
VERSION     DAZUJO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.055"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..725)
                     /locus_tag="ACG8OL_001915"
     CDS             complement(27..725)
                     /locus_tag="ACG8OL_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS4817899.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="ACG8OL_001916"
     CDS             complement(718..2070)
                     /locus_tag="ACG8OL_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS4817900.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="ACG8OL_001917"
     CDS             complement(2293..3693)
                     /locus_tag="ACG8OL_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS4817901.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="ACG8OL_001918"
     CDS             3854..4591
                     /locus_tag="ACG8OL_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS4817902.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="ACG8OL_001919"
     CDS             4716..5195
                     /locus_tag="ACG8OL_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS4817903.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="ACG8OL_001920"
     CDS             5192..5959
                     /locus_tag="ACG8OL_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS4817904.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="ACG8OL_001921"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="ACG8OL_001921"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS4817905.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="ACG8OL_001922"
     CDS             complement(6862..7464)
                     /locus_tag="ACG8OL_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS4817906.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..7800
                     /locus_tag="ACG8OL_001923"
     CDS             7570..7800
                     /locus_tag="ACG8OL_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817907.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7791..8039
                     /locus_tag="ACG8OL_001924"
     CDS             7791..8039
                     /locus_tag="ACG8OL_001924"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4817908.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8145..8729
                     /locus_tag="ACG8OL_001925"
     CDS             8145..8729
                     /locus_tag="ACG8OL_001925"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS4817909.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="ACG8OL_001926"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="ACG8OL_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS4817910.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="ACG8OL_001927"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="ACG8OL_001927"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS4817911.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="ACG8OL_001928"
     CDS             10630..10809
                     /locus_tag="ACG8OL_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817912.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="ACG8OL_001929"
     CDS             10831..11856
                     /locus_tag="ACG8OL_001929"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS4817913.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12047..13291
                     /gene="glyA"
                     /locus_tag="ACG8OL_001930"
     CDS             12047..13291
                     /gene="glyA"
                     /locus_tag="ACG8OL_001930"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS4817914.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13444..14391
                     /locus_tag="ACG8OL_001931"
     CDS             13444..14391
                     /locus_tag="ACG8OL_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS4817915.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14525..15154
                     /gene="upp"
                     /locus_tag="ACG8OL_001932"
     CDS             14525..15154
                     /gene="upp"
                     /locus_tag="ACG8OL_001932"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS4817916.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15293..15535)
                     /locus_tag="ACG8OL_001933"
     CDS             complement(15293..15535)
                     /locus_tag="ACG8OL_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817917.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15656..15728
                     /locus_tag="ACG8OL_001934"
     tRNA            15656..15728
                     /locus_tag="ACG8OL_001934"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15689..15691,aa:Thr,seq:cgt)
     gene            complement(15847..16485)
                     /locus_tag="ACG8OL_001935"
     CDS             complement(15847..16485)
                     /locus_tag="ACG8OL_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS4817918.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16608..17240)
                     /locus_tag="ACG8OL_001936"
     CDS             complement(16608..17240)
                     /locus_tag="ACG8OL_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS4817919.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17591..17890
                     /locus_tag="ACG8OL_001937"
     CDS             17591..17890
                     /locus_tag="ACG8OL_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4817920.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6160 a         3149 c         3557 g         5118 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg tagattacaa
    12061 aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac aagaacataa
    12121 tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg cacaaggaag
    12181 tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg gtgggtgtga
    12241 atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat tcggagcgaa
    12301 atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt atctcgcttt
    12361 ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac atctaacaca
    12421 tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg gtgttgatcc
    12481 gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac atcaaccaaa
    12541 attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga aattcagaga
    12601 aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg caggattagt
    12661 agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga ctaccactca
    12721 taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag cattagcaaa
    12781 aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc atgtgattgc
    12841 gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat atagtgaaca
    12901 aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg gaaccagagt
    12961 catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt taggtataaa
    13021 tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata aaaactcgat
    13081 tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca cgccagcaat
    13141 cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag ttgtcaaagc
    13201 attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg tacgcgaatt
    13261 aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt aaacagaaaa
    13321 gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga caatatcggt
    13381 gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca gggaggaaaa
    13441 gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga tgcaaatgcg
    13501 cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact agaagaagta
    13561 caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt ggatactggc
    13621 gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga taaattacca
    13681 gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac ccatgataaa
    13741 ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga atatagtcga
    13801 tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt gcaacagctg
    13861 aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc aggagattca
    13921 gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa gggcctccca
    13981 atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac agattcctat
    14041 catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt ttgggaccaa
    14101 tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag agcaaatata
    14161 aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga tgtattaaga
    14221 gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt tacacaagta
    14281 cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca gacaaaggca
    14341 acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata agctttgctt
    14401 gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc gagtttgact
    14461 agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa ggagagctta
    14521 aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac taactattat
    14581 tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa tcgctatgtt
    14641 gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg aaacaccgat
    14701 cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc caatcttgcg
    14761 cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta aagtgggaca
    14821 tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg tcaaacttcc
    14881 agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa caggcggatc
    14941 agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta aatttgtttg
    15001 tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg atattgacat
    15061 ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc ctggactagg
    15121 ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg gtgtgacaca
    15181 acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag ctttttcaca
    15241 gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa attcaaatct
    15301 tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc aagttcgcat
    15361 tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt gaaatcgttc
    15421 cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg aatttttcac
    15481 caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt ttcataaaac
    15541 ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt aaactaattt
    15601 tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg ataaggccag
    15661 cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt cgatccctat
    15721 agctggcatt aaaagagaag acagatatat caaggatttt taatccattg atacatctgt
    15781 ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta ttatttctat
    15841 aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata tcacatgaac
    15901 tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata acaatcttgc
    15961 caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat tatcataagt
    16021 actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag gaatgctgaa
    16081 cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa gtatcatgat
    16141 aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc ctgttaaacc
    16201 ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta cattgataaa
    16261 aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt ttttcgtatc
    16321 ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag aaaaaatgct
    16381 aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa tagtgcccaa
    16441 tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa taactcctac
    16501 tattcagttt ttgaagatag agtcagtata catgaagagt taaaataggc aatgtttttt
    16561 tatggatatt tgtattgcta aaaatttatc ttatatattc attttgatta tactactttt
    16621 atttgtaaaa attttctagc aaaaccaagt gcttgcaact gattaagcgt acaatttttt
    16681 agatgttccg agcgtacttc aatcatttca aaggagttgc tacttaaatc gatatttttt
    16741 aaatcagtat caaaaaagct agcattttct aaatcattag tagaaaacat tattttagtg
    16801 agggaacagt ctacaaaatc tgcgcttgat aagacagagt gctcaaaggt actttctttt
    16861 atgtttgaac cagataaatt tacatagtcc attttacaat tctcgaataa cgtgttccca
    16921 atataacttt ccatcaattt agtacctgtt aatttacaat tgacaaattt cacgcgatgc
    16981 aacatgcttt ttgtcaatat acagcctgaa aaatcacagt tttcgaatat acagtcggta
    17041 atctccattc tttgaaaatc attggaagca aaaaatgcat acttgaaaat agatttgtaa
    17101 ataattggat ggcagattat ttggttttcg atgacctctt tttgtgaaag gatattatac
    17161 agttcaaaat cttcgttttc atagatatga aaaaaggatc ctttattcaa gcttgcttca
    17221 ttaactttag gaaaaatcat atttaattac ctccattttc ataaagtcat attaacaaaa
    17281 gctaaaccaa cttacattag cagaaaatga gataggtttt cgcctttttc gataacgata
    17341 gtgtcgctat ctcttgaaac gttcttagaa ttgccctgag agacacaaat taggcgtaat
    17401 tctaataaaa atcttagaat aaaaattttt actgatttaa tttagaatgt ttttaaaata
    17461 aaaatcgttg ccataacagt gtattgaaag gttttaacaa tccagtaatg atttaaaaaa
    17521 atctagatgt agcgtatact ttgtttatag aatgtagtgg gaagaaatat atttttggga
    17581 ggaaatcgtg atgtctaaaa gtgaagaaat agaaaacatg gtactaaaat tatatgatct
    17641 cgttttaaat ccagagatca aagaaaagga acgtatactt ttgatagacg caaagacagg
    17701 cttagaaaaa ggtcagtatt atcctaaagt cataaataat ttggaacgta gtttacgtcc
    17761 gttagcaatc aggggagaat tgtctaaacc agtatcaccg ttttatatgg agatatcaac
    17821 aataggaaaa tttgaaaaag aattaggtcg aggaatggct tcggcaccga ttacttttgg
    17881 gcatttataa aaacgagtta ttcatctcaa tgaattaaaa agtatagtca atctcataat
    17941 gcaggctagg ttcatttatt tgaacagagt ctgtattttt ttat
//
LOCUS       DAZUJO010000055        16931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000055 DAZUJO010000000
VERSION     DAZUJO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.056"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8OL_001938"
     CDS             complement(451..1761)
                     /locus_tag="ACG8OL_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS4817921.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACG8OL_001939"
     CDS             complement(1773..3191)
                     /locus_tag="ACG8OL_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4817922.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACG8OL_001940"
     CDS             complement(3431..4999)
                     /locus_tag="ACG8OL_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS4817923.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACG8OL_001941"
     CDS             complement(5003..5413)
                     /locus_tag="ACG8OL_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS4817924.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACG8OL_001942"
     CDS             complement(5410..6333)
                     /locus_tag="ACG8OL_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS4817925.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACG8OL_001943"
     CDS             complement(6330..7208)
                     /locus_tag="ACG8OL_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS4817926.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACG8OL_001944"
     CDS             complement(7195..8532)
                     /locus_tag="ACG8OL_001944"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS4817927.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACG8OL_001945"
     CDS             complement(8535..9638)
                     /locus_tag="ACG8OL_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS4817928.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACG8OL_001946"
     CDS             complement(9712..11220)
                     /locus_tag="ACG8OL_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS4817929.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACG8OL_001947"
     CDS             complement(11186..11878)
                     /locus_tag="ACG8OL_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS4817930.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACG8OL_001948"
     CDS             complement(11900..12874)
                     /locus_tag="ACG8OL_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4817931.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACG8OL_001949"
     CDS             13174..14046
                     /locus_tag="ACG8OL_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4817932.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14628..16826)
                     /locus_tag="ACG8OL_001950"
     CDS             complement(14628..16826)
                     /locus_tag="ACG8OL_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817933.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4983 a         3541 c         2749 g         5658 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagctt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattaaacgg cgtttatctt
    14521 gttgattctg cggcaataaa atcagccagc agcaaaacat gaagtgctgt tttttgctgt
    14581 cctcttttat tgcttgaatc agatcacaag aaaaacagcc tcttgattca caaatatttt
    14641 tttcgatatt ctcccggcgt aatcgattcc ttctttttaa agaaacgaat aaaattttgc
    14701 ggatttcgat attgcaatct ttctgcaatc tcttttacgg agaggttagt ctctttcaac
    14761 catttttttg ctacctctaa acgatgattt tgaacaaaat caccaaattt ttctcctgtt
    14821 tcctttttaa atacattact taaatagtta gggttataat gcaacctatc agcgatcaca
    14881 tctaaagaca gatcacgatc atattctgtg tagacgatat gcataatatt ctccgacaaa
    14941 tttttaaatt catgatcagt gattgcttga gagcattctg tgattggcag aactaattgt
    15001 tctaaaacaa gtttttctag ttctttggga tgataattgt tcagtgcttt tacataaatt
    15061 tgtttcatgt tatcaaagtt ttttgaatct actcccaata actgtcctaa ttgaacgagt
    15121 tcattgatca agcggaccga aactacttcc cgactcaaag gatttttatt ttgtgcaaac
    15181 aatgcatcaa tcaattcatg tactaaattc ttcacttcct gatttccaga acgaatggct
    15241 tcaaacaatc gattttgttc ttctatcgga tatctaataa gtgttttttc atgagcagca
    15301 ggaacgattt cctgataaaa aataattgaa cttggaccag tatttacacg ataatacagg
    15361 gcttcttttg cccgatcaac cgattgcttg ctttcattca gcgtttcgaa ccggttactg
    15421 atgcctatac tgatagttaa attcaagtat tcttttacac gtttttgaat gagtcggcaa
    15481 tactccatga cttttttgct tgcttctgca tcatctttat ccattcgata aatagtggct
    15541 tgcatttctt cattcaaaac gatcggcagc attcgatttt cctttggcac aatttccgtg
    15601 atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc caccgtgact atcttctaac
    15661 agatcgattt gaaccaaagc tgtataatag cagtcatttt gagaactata accaaattgc
    15721 tgcaaccgct gatctagttc tcgcctttct actcgatttc taaataaact aagtacaaat
    15781 agcgtttcca gctgtggctt ttgggtaact aagtgtgcac ttaacgcttc tttttctcct
    15841 atgatccgac tgactgattg agcgaccaaa tctagttctt tcccactgaa tccttttgat
    15901 ttaagattca gacgttcctg tatttgagaa atcggacggg caaatctgtc cgaaaataag
    15961 taggaaaaaa gaccaatgag tcctaccagt gttaaagaca ctgcaactag cccgattctc
    16021 agattgcgga tcattgcgcc aattgcacta gagtcaactt ctaatctgta aatccaatta
    16081 ttataatctg atttcagtac tatatacgtt ttatcttttt ctcttatcac tttcagctga
    16141 ttgtaatcta gatccacgtc cgttaataca gttggttgct ctttattttt tactgtctga
    16201 gtagtaaaaa ggttgccaac ttcgttttca atagaaagta tgtcttctcc actttcacca
    16261 acaacttgtt ctattgtatg tttctctatc gtagcaattc ccaatgcata tttttctttt
    16321 tcaaacattg gcaaagaaat caccatttcg attcctttgt ttttcttttg ccaaaataaa
    16381 ttatttttgt tatttatgta cttctgttga tacttttcaa cttcctcttc ggtcagttga
    16441 ctcaacgatc cattgattac tccccattta ccagccaaac tgactaaatc ataattatta
    16501 tcatccataa caatcgtttc tataaaattt agttctttcc tgatttcaga ataagtggag
    16561 taatctgtat atgtcagcgg atgcttgaat atgtccgaga aatttttggt cgaaccatac
    16621 gtagaaaacg aatattcaat cgttctaatt ttccattcaa cgttagtttt aacttgcctg
    16681 agcaaattct ttttgtctgt taaataactc gattcgatcg attcataggt attctggtaa
    16741 atgaataaac taaatcctat gacaagtacg gctcctaata aaaacatcgg taaaaatatt
    16801 ttataaaata ctgtactttt cttcattttc ttcctctcct ccttttaaaa taaaaggctt
    16861 tcatttttct gtaaaattga tactcatcta attaaggctc ttcgtcaaat tgtgttggcg
    16921 aagacaaatt t
//
LOCUS       DAZUJO010000056        16919 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000056 DAZUJO010000000
VERSION     DAZUJO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.057"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..2134)
                     /locus_tag="ACG8OL_001951"
     CDS             complement(242..2134)
                     /locus_tag="ACG8OL_001951"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817934.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2131..3858)
                     /locus_tag="ACG8OL_001952"
     CDS             complement(2131..3858)
                     /locus_tag="ACG8OL_001952"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4817935.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3874..4524)
                     /locus_tag="ACG8OL_001953"
     CDS             complement(3874..4524)
                     /locus_tag="ACG8OL_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS4817936.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4728..5024
                     /locus_tag="ACG8OL_001954"
     CDS             4728..5024
                     /locus_tag="ACG8OL_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS4817937.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5196..5816
                     /locus_tag="ACG8OL_001955"
     CDS             5196..5816
                     /locus_tag="ACG8OL_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4817938.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5908..7074)
                     /locus_tag="ACG8OL_001956"
     CDS             complement(5908..7074)
                     /locus_tag="ACG8OL_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4817939.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7086..7439)
                     /locus_tag="ACG8OL_001957"
     CDS             complement(7086..7439)
                     /locus_tag="ACG8OL_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS4817940.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7567..8589
                     /locus_tag="ACG8OL_001958"
     CDS             7567..8589
                     /locus_tag="ACG8OL_001958"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS4817941.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8608..8769
                     /locus_tag="ACG8OL_001959"
     CDS             8608..8769
                     /locus_tag="ACG8OL_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817942.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9028..9462)
                     /locus_tag="ACG8OL_001960"
     CDS             complement(9028..9462)
                     /locus_tag="ACG8OL_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817943.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9572..11431)
                     /locus_tag="ACG8OL_001961"
     CDS             complement(9572..11431)
                     /locus_tag="ACG8OL_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4817944.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11432..11572
                     /locus_tag="ACG8OL_001962"
     CDS             11432..11572
                     /locus_tag="ACG8OL_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS4817945.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11549..12709)
                     /locus_tag="ACG8OL_001963"
     CDS             complement(11549..12709)
                     /locus_tag="ACG8OL_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4817946.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13016..13927)
                     /locus_tag="ACG8OL_001964"
                     /pseudo
     CDS             complement(13016..13927)
                     /locus_tag="ACG8OL_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            complement(14093..15286)
                     /locus_tag="ACG8OL_001965"
     CDS             complement(14093..15286)
                     /locus_tag="ACG8OL_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4817947.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(15370..15570)
                     /locus_tag="ACG8OL_001966"
     CDS             complement(15370..15570)
                     /locus_tag="ACG8OL_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4817948.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(15994..16227)
                     /locus_tag="ACG8OL_001967"
     CDS             complement(15994..16227)
                     /locus_tag="ACG8OL_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817949.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(16234..16665)
                     /locus_tag="ACG8OL_001968"
     CDS             complement(16234..16665)
                     /locus_tag="ACG8OL_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4817950.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4751 a         3696 c         3178 g         5294 t
ORIGIN      
        1 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta aggaagaggt
       61 tgtaaaatca gcggtttgac ctgagtatta gagatacaaa acggaaaaat agcttctcat
      121 attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccgt ttattcggaa
      181 aaagggactg tgacacgact tttgtcacag cccctaaaaa aaataataaa tttgtactat
      241 tctatgaagc ttcttcttca gaaaactgac tgttataaag atctgcatag aatccgtctt
      301 tttccatcaa tgtatcgtgg tttcctgttt ctacgatcga accatgattc atcacaataa
      361 tgttgtctgc atcgcgtatt gtagacaagc ggtgagcaac aacgaaactt gtccggtttt
      421 ccaataggcg gttcatcgct ttttgaatca gtatttctgt tcgtgtatct acactagatg
      481 ttgcttcatc aaggatcagg acatctggat ttgctaagaa tgcccgcgca attgtgatta
      541 gctgacgctg cccttgcgaa atattgcttg catcctcgtt taatacagtg ttatatcctt
      601 ctggcaattt acgaacaaaa tcgtctacgt gagcagcttt agcagcctca atgatttgtt
      661 cgtctgttgc atcgtcgttc ccatatttga tattgtcata gatacttcct gtaaacagcc
      721 aagtatcttg cagtaccatt gaaaactgtg cgcgcaattc atcacgtgaa agatcacggg
      781 tatctacacc atcgtaacga atactacctc cagaaacatc gtagaaccgt tcaagaaggt
      841 tgatcagtgt cgtcttccct gcaccagtcg gacctacaat agcgaccatc tcaccaggtt
      901 tgacatccaa gttaaaatca gtcattagca agttatctgg tgtgtagccg aattgaacat
      961 tttcaaaacg aactttgtat ggtgtatctt tttcttctgg aaggccagat ttttcattgg
     1021 acatctcttc ttcatccaac acttcaaata cccgttcggc tgaagcaact gttgattgga
     1081 tagtgttcat caaacttgca agttgagtga ttggttgaga gaactgtgtt gtatattgca
     1141 agaacgcttg aacatcccct aatgtcatgt tcccatttgc aacttttact ccgcctagta
     1201 cagcgacgaa cacatagcct agatttttaa cgaaattcat caacggcatg atgattgccg
     1261 aaataaattg agctttccaa ccagcggcat acagattctg attttctttt tcaaatactt
     1321 cctggtcttt ttcagtatga ttgaatgttt taacaatggt atgaccacca tatgtttctt
     1381 ctacttggtt attcaataaa cctaaacttt tttgctgatc tgcgaaaaac acttgtgatt
     1441 tcggcgctac gatcatcacg atgatcaagc ttaaagggat catcaataac gcgactaacg
     1501 tcaattgcca gctgattgaa agcatcatca cgatgacccc aatcaatgtg actgcactag
     1561 ttacaaattg agtcaaattt tgctgcagcg tgcttgcgat gttatccata tcattgattg
     1621 ctcgagacat gatatcccca tttgtatgtg tatcataata ggaaatcggt acacgattca
     1681 ttttggcttc caaatcatga cgtaattcat aaactgttcg ttgggaaaca cgtgtcataa
     1741 taaattgctg taaaaagttg aatacagcag aaatcaaata catcacgatt actgtcgcaa
     1801 tgatctgcgc gattttatca aagtctatcg gaaattttgt tacttgttgg ccttgttgca
     1861 tctgttgagc gccagccatt acacctttga atatttctgt ggttgctttc cccaagacct
     1921 ttggtgtttg gatttggaag atcacggctg ctattgctag tacaaataca gcgatgatcg
     1981 caaccatacg tttagacata tagccaaaca atcggcgtac cgttccccag aaatttttag
     2041 gcttttcacc ttttacaccg agatttctgc caggtccacc atgtcctgga ccgcctgctg
     2101 gtccatgaga gggacgtgtg tttgttttac tcatgctaag tcctcctctc ttagttgtga
     2161 atgaacgatt tcttgataag tttcgtttgt tgctaatagt tcttcatgtg ttcctttgcc
     2221 aaccagtttt ccttcatcta gcacaaggat catatctgca tccattactg tacttactcg
     2281 ttgagcaact aaaacggtga tactttcttt catattcgtt ttcaatgctt ggcgaagatt
     2341 ggcatccgtt ttgaaatcca acgctgagaa ggaatcatca aacacataga tatctgctgt
     2401 tttgactaat gcccgtgcaa ttgccagacg ctgtctttgt ccaccagaga agtttcctcc
     2461 tccttgttcc acatggctat ctaagccatc ttccatctct gaaacaaaat ctttagcttg
     2521 tgcgatttct aatgctttcc aaatcatttc atctgttgca tccggcttac catactgcat
     2581 attgtctcgg atcgttccag agaacagaac tgctttttga ggaacaaagc ccattaattc
     2641 tcgcaaatta tattgagtca tgtcactaat gtccgttcca tttatttgga tcgaaccaga
     2701 ttcgatgtca tataatcgag ggatcaaatt gactaaggtc gttttacctg aaccagtccc
     2761 ccctatgatt gcgacgatct cgcccgactt ggcttggaaa tcaatatctt ccaatgcaag
     2821 tttttctgct ccatgatatc gataattgac atgattgaag gccaatgttg cgttttttcc
     2881 agcaaaagaa actgttttag gattttcagg gtcatggata ccgatttttt catccaacac
     2941 ttcattaata cggtcagctg aagcttgtgc acgaggcacc atgataaaaa tagcggaaag
     3001 catcataaag ctgatcaaga tctgcatcgc ataagtcata aatgcaatca gattaccgac
     3061 ttccaaagtc atatctgcta tgtagtgtcc accaaaccaa gtgattgcta cgtttgtacc
     3121 gctcatgatc aatgtcatca ttggcatcat caatgcgacg atcgtattaa ctttgattgc
     3181 cgtatttgta taatctttat tcgctaaatc aaaacgattt tcttcaaagc gtgtcttatc
     3241 gaatgctcga atcactctga cccctgtcaa tccttctcga aataccaggt ttaaacggtc
     3301 agttttcttt tgcatacttt tgaataatgg aacagcaaag tacatgattc cgccgatcaa
     3361 aataatcatt actgggatga caactaagaa gatttttgtc aactgatgat ctttttgata
     3421 agcaagtatg ctcgcgccaa tcaacgtgat tggtgcattg atcatcatac gtaaaaacat
     3481 ttgtgtaacc atttggattt gagtcacatc attggtcgtt cttgtgatca aagaggccgt
     3541 tccatattta tcaaacgcat cttttgtcag actctctgat tttttataga catccgtacg
     3601 caattgctta cccagtttct gtgattcacg agtagcaaaa aatgtatttg cgatagcagc
     3661 taagatactg atcaaagaaa agccgatcat cacaaaacct gtatgccaaa tataatcgac
     3721 atctccttgc gctacccctt tatcaataat attggaggta agtgtcggca aatataaatc
     3781 tgccagtact tggatcacca tgaaaactgc agctgcaatt gctgaagtca aggagatccg
     3841 ttttacgatt tttagcatct agttcccttc tttctattta tatactccgt ttttcagcca
     3901 agaaagtttc aaattgaatt cactgattgc ttgctctact gagtaatctt cagatgttga
     3961 aaataagcga tacgaatgcg caattgttgt aaatacagta ttcatcacta attggatcag
     4021 taattccaat tcatgatcat tttttacttt cagcttcgtc cgatcgatca attcaagcaa
     4081 ctcttttgca tcttgtttgt gttctttatg tggatcgtga ggcgtttttt ggcgactcgt
     4141 atgaggagcg acttttcttg aggcacggta atccatgttc ataaacaagt tacgataaaa
     4201 agaagcattc tcgccttcca aaatctcata aatcatttta gaaaaatagg attcgaatcc
     4261 tttgaataga tcaccatcgg attctttcat tagttgtaac atatctttag agctgtcacg
     4321 tctcattgtc tcaaagtaat aaaaataaag atcctcttta tcttcaaaat actgatagaa
     4381 actgcctcta gggatatccg ctaatttgac gatattagca atcgaagctt cttttaaggg
     4441 tactctagaa aactcgattc tcgcagcctc gattagtctt ttttgttttt ctttaggcaa
     4501 atggaaaaat gtctgtgtcg gcatatggat cctcctttct ttcaagatga cacattgtca
     4561 tatgacgctg tgtcattata acacctccta cctgttttgc aagacatttg catagttttt
     4621 ttcattatca tccgatacat taaaaaagtg ataagaggaa tccttccatt tgaattaatg
     4681 agagttgccc attaaaatag ccacatacta aacaaaggat gagataaatg gcccaatcaa
     4741 ttagtgtcca agacttcaat aaattgcctc tcgatgctga cagcatcgtt ttagatacaa
     4801 gaaatacctt agattacaca aaaaaccact taagccactc gctttctatc cccgctgcta
     4861 tcttgccaag acaattaaaa aatctagatc caaagaaact ttattatatt ctaagctata
     4921 caggcaggcg atcagaagca ttagccgatc aacttactgc taatggtttt cgtgctgttt
     4981 atatcattgg tgggatggaa gctcttcagt cgagtgttgc ttgaatattg cttgttagat
     5041 tcaaaagagg ctgtgacaaa aaatcgtgtc acagcctctt ttataccctg tcttttttta
     5101 gttatttatt cttcaacctt atcaatatga cttgatagcc ttttcttttt ctcatagtta
     5161 taacaatcat agcaataaaa ggagaaggta agcatatggc atctgtttta gtagtcaaag
     5221 gtcatcccct aactgcagaa gaatcgcgca cagttaaggc attgacgtct tttttaacaa
     5281 gttataaaga aaatcatcca gatgatgaag tgactgtttt ggaactttat cgcgatgata
     5341 ttccggaaat cgatgaagaa ttattatcag gttgggaagc attacgtgct ggtgcagaat
     5401 tcacttcctt atcagaaagt caacaacaaa aaattgctcg tttcaatgaa ttgactgatc
     5461 agttccttgc tgctgataaa atcgttatcg caaacgcctt atggaatcta aatatcccaa
     5521 caaaattaaa agcttggttc gataccgtca atgtagctgg aaaaacgttc cgctatacag
     5581 aaaacgggcc agaaggatta gtgacaggta aaaaagcact gcacatccaa tctaatggcg
     5641 gtgtatacaa cggacaagac tttgcctctc aatatgtaaa aggtatttta aactttgtag
     5701 gcatcgatca agtagatcag ctattcatcg aaggaatcga ttatgaccct gaccgtgcag
     5761 atgagttgat gcaaaatgca ttagataaag ctgctgcttt aggtaaatca ttttaaaaat
     5821 tgagataaat gcatacaaaa gagacagagg aaaaaatctt caaagcagca tttgtacctt
     5881 ggatattttc ccatctgtct ctttttgtta ttctttctgc tttttcaacg gattttcttc
     5941 ataaagagaa gagattgttg tgtaccattc aaaaacatga gtaatcacta ctttagctgc
     6001 tgcataaaca ggaatcccca agatcacacc gatgattccg aacaatttcc cagaagtcag
     6061 cagaactaat aaaatcgtta ctggatggat tgctaactga ttccctagta ctaatggaga
     6121 gatcaagcgt ccttctattg tctgctctac tgcaaaaacg atcagtactt tgatgatcat
     6181 gaatggacct gctacaatgg ctaaaaagat agccggaatc atcgctaaaa atgatcctaa
     6241 gtaagggatc aggttcaaaa aacccgcaat aatccccaac gtgacagcat actctaaacc
     6301 aatgaccgca aaaccaatca taaacatgat tgccacagca aaagccacag ttagttgtcc
     6361 tcttatataa gaagaaactt gactattcat ttcactcagc acattcaacg tcggtttttg
     6421 catgcgtgtc ggtaaaaatt tgacaaaata aggtgctaat tgtttaccgt ctttcaatag
     6481 ataaaataag ataaacggca tcgtcagcag agccactaaa atagtcgcta ccgcgccgac
     6541 aaaacttcca agactctgaa ctgtactctt agatatatct tggatcatat cgcctgccga
     6601 attcatgaac ttatcacctg ctgtttccaa ctgttcgcga aattggttga acagcgggtc
     6661 ccgcaaaatc tcggttaatt tattatcaat cgtatctaca tattgaggga agttactgat
     6721 gaagctcact gtttgttcct gaatcttagg gatgatcact acgctccccc atgcgattag
     6781 tgcaacaacc aaaacaaaca gcccaatgat actgtaaagc cttggtactt ttcgcttttc
     6841 aaagtaatca actaccggat tcatcaagta atacaagatt cctgctaaaa tgataggcag
     6901 acctacgatt gctaaaaatt gccagattgg tgcaaacaaa tatgaaactt ttgtaaataa
     6961 aaagacgatc agcaatatta acaatacaac aagtaaggct gtgactactt gattatttaa
     7021 aaaccagcgc caaaaccacg ataccggtct gttcttctta ttttttcctt ccataattcc
     7081 tcctgttagt tataaaaaat ttggcttccc aaaccaatag caggcagtct atgcggatga
     7141 agatagattc catttgcaat ttgtccttct tctggacaag catcaaaaat caatgatacg
     7201 tgagctactg tctctaaatt cccattggcc atcggaccga cataatcgat cgtatatacc
     7261 tgatcatcaa aagcaattgt tccgcctgct ttcaaagaaa aaggttcgtc ttttgatact
     7321 tcttgaatca ccgaatattt tcgtaaggca gctgttgctg ttttatcgaa caaaatcagc
     7381 atgggttctt ccttgtttat tgcttctgtc ccaatttctg taatctttgc ctgaatcatt
     7441 gcactctctc ctttaattgc tttcaaaaac agtataacac attctcattc tttagccgtg
     7501 tggaataatt atgtgataga ataaaaacga aaactatttc attacaatta ggaggaatca
     7561 gctcatatga tcgaaaagat aattttagga acctatacgc gtcgcgaaag taaaggaatc
     7621 tatacgatcg accttgatac agaaaaagaa gaattagcca acctttcatt agctgttgaa
     7681 gaaaatagcc ctacctatgt agcaaaaagt aaagctggca atctttacac agttactagt
     7741 gtggatggat taggtggtgc cggtgcgtac gataaggatt ttcatttttt gaatgcggta
     7801 acagagtctg gcgctccttt gtgttatgta gcggttgatg aagcacgtca attactatac
     7861 ggggcgaact atcataaagg tgaagtaaat gtctaccata tattaggaga tggcgaactg
     7921 aaagcagcag attcaatatt ccatcaagaa gcgacaggtc ctcataaaaa ccaagatcat
     7981 gcgcatgtcc actatacgga tctgactcct gaccaacgat tagtcgtatg tgatcttggt
     8041 acagatcgtg tgtatactta tgatgtgtcc gaaaacggca aattgaatct tgtaactacc
     8101 ttcactgcag agccaggaac aggccctcgc catcttgtct ttcatccaaa tggacaattc
     8161 gcttatttat ttggtgaatt agacagcaca gtccgcgtgc tttcttataa taaagaaaca
     8221 ggtgcttttg aagaattaca gaaaatatcg accttgccag aagaattcga cggtgaaaat
     8281 ggaggggcag ctatccgcat ttcaaatgat ggcaagttcc tttatgcttc taaccgagga
     8341 cataactcga ttgctgtatt cgcagttttt gaagatggtt cccacattca aaacaagcaa
     8401 atcatctcaa cagaagggga ttttccccgc gatttcgcac tagatccaag caataatttt
     8461 gtcgtgtgtg ccaatcagaa caccgataat ctgacattat atcgccgtca tcctgaaaca
     8521 ggattactgg aattactgaa aaaagatgtc tatgtaccag aatgtgtctg tgtttatttt
     8581 gatcgttagt aagaaaagga gaatgaaatg aatcagcaag acgtattaga cagactacgg
     8641 gaagaactaa atattccttt ctttgatggg aaaatcgaag ataaagagta tagcgaagag
     8701 gaatatcaga aactcaaagc cgatttacag aactactttg aacaatatgt acagaatgta
     8761 gaaaactaat cagctttcca tactatctga aaagaacatc aggacaagac ttttccgtct
     8821 atcctgatgt tcttttctac aaataaaaaa ccgccgagat ggttagactc gacggaaaag
     8881 gagtatttac tcgattttga gtaagtacaa ggttgttgtt ggttataggt atgtcattat
     8941 cataaaggat tttgatcact ctatcataaa ggtaatatta catttatatt agataactct
     9001 agtttttatt gttatcaaag atttctttta ttttttcaac aacaaattag tcattttttg
     9061 gatggcagct gtcaattggt ctgtcgcttg gtcttgtttt gcttcagatg tattgaatag
     9121 atcttcatct acccctgatt caagatcgat actttggatc atttcttcac agcctgctac
     9181 atatgaaaga taagcttttt caaaattttt atgaatgccc attgcttttg ctggtggacg
     9241 caaagtactg actttcttaa gcattgcccg atattttttt gttccttctt caaataacag
     9301 gatgatctct tcaagagtcg atttgtccag ttcacttgtt tttccttcat caattgcgtt
     9361 tctgatcact tcatatttcg gatgcattcc ttctccgatt gcttctgttt ccttgacgat
     9421 ttgatcgatg gactgcacat aatagccaat attcggtaac atgttcgttt tcctactttc
     9481 tccattgttg ttattattta ttctacttgt ttttatcctc aaatgacaag tacttgaaaa
     9541 aatttaatgg tttctataca gctcgccatt cttattcgtg ctcaaagaaa ccatctgcta
     9601 aactatattg tttccctgcg acaatgtcat attggatcaa ttgatattcg tcgtaaatct
     9661 cttgttgcga gcgattgaat tgcgcttctt tataccactg tccgttttga tagtacagtt
     9721 ctcgttcaaa cgccggaagt tcctgttcta atgataaaag cagctgataa aagccatttg
     9781 ttgtctgatt cgtctgttcc attagattcg ctgcaaaata aaacggactg gtgattgcat
     9841 cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa cataagaaat tgtgtttcat
     9901 gcaatgtttg tttgtcattt tttgcttgga tcgtatctga atagatccct ggaagatgat
     9961 ccccccaaaa aacaactaaa gtccttctag aaagattctt caattcttcc gtgaactgct
    10021 tcagtgccgt gctgctatag gaaatatctt gcagataatt ctctaagtca agtgtcccac
    10081 ttccatctga agtttttgca ggatagtcta gattatcata ctttccatcg tagggcatat
    10141 gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt ttcttccttg agaagtgtca
    10201 ttacttcttt gtaagctgac tcatcagaaa tatatggatt attctctatc gtatccgtat
    10261 aggtcatcgt acgctcgctg atgaactggt caaatcctaa tgtctgataa acatcctctc
    10321 ttttatacat cgaagtattg tatggatgga tcgctgtcgt ctggtatgat tgtgcgttta
    10381 acgctgaaac gatagaagga agctgatcca tttttggtac aagcatcgtg taaggggtgg
    10441 tgagttgcgc attaaataac gccatagaaa aaccagtcaa tgcttcaaat tcgatatttg
    10501 ctgttccacc gccatagttt tgagaaagca tattgccact atatgtttga tcagctactt
    10561 cataataatc tgccaatggc tcaccagata cttctacgcc gtttaatcga ctaggatctg
    10621 agaaactttc actcatcacg aacacgatgt tcggcgactc ttcctctaca gttttatttt
    10681 tttcatcggc tagcttctgg tatttttcag tgatttcttt gatcttttct ttcgaatatc
    10741 cttcaggttc atccattggt tctaccttca gattgtacaa aaacccaccg ataaaaccag
    10801 tgttatagta gttcattttc tggctgtaag gtatccatag tgccgtttta ttatatgctt
    10861 ttcgcaacaa attatcggga ttgttgaaat tactgatata ccctagcagt ccaattgcca
    10921 caagcagtgt cgttaatcgg atcaattgga atcgccttcc ttttttcagg cttcgaaaca
    10981 tgtaccagca aaacaatcct atcacggttc ctgcggcagc taagatcact gtaaacagca
    11041 tagtgccggt catatctttc agcaatccga tttcagtgat catcttcaaa tcgtctggat
    11101 aaatcggttc ttgccgataa ctcattttca tataatttgc aaaacctaat acaccgatgg
    11161 ctacgacata cgtaagggaa cctaaaggga cagatcctgc tagtgcaatc agaaaaataa
    11221 gaataatcag caagacgaga caggctaaga agaatttttc cgtatgccaa gaaaaagcaa
    11281 atttcaacgc aaggtctacg gatagatcat tttgacacca ctgcaaaaat aaattactaa
    11341 aaacaacaag aaaaaagata ctgatgattg ctgctgttcc ttttaaaatc tttttctgtt
    11401 tggtcgaaag tctgcttata tatcttttca aatgcttgct ccttcatttc tatcaataaa
    11461 agaatgttgc ttctcttatt ttagttgtac gaaattcgaa agtaaagaac ggaaagaaga
    11521 attaatgaaa tccaaatatt ctctttcttt atggcctttc tggtttcttt aataagcgga
    11581 cctttcgtac tccagaaatt ttttctagct gttgtttcac ttgattgagc tgttcaaaat
    11641 cagtactgtc tatgtcaatc aatatgtaga catgctcatt cttatggtta cgcgatatat
    11701 ctgcgatcgc tagctcgttc tcattcagca gagaaaatat ctctgctaat atattggttt
    11761 tcttttgata aaatatagtg aatcgaaacg gcgcttggaa aaacattcgt gcggatggat
    11821 aattgaccgc ttctctaacc gtaccgaaca aaagataatc ttttaaggct tcaactgcca
    11881 aacgtccgct gtctgccaaa gcttcctgag tcgatccccc tagatgaggc aacataagta
    11941 tacggtcatt ttctaagaac tcttcttctg gaaaatcagt caagtattta gccagatgtt
    12001 gtttttccaa tgcttgcata acagcatatt tatcaacaat ttcacttctt cctacattga
    12061 taagtacagc atcttttttc attttttcta tattcgcctg atcgatcaat ccttttgtat
    12121 cttctgtcaa cggcagcatg actacgatgt aatccgaggt agaaagaact gtttctaaat
    12181 ctgcttgccg aacataatcc aactgggcat cgcggataga ataacccaag acatccatcc
    12241 ctaagctata gcaacttagc gctacttttg tgcctatcgc tccaagacca agaagaccga
    12301 tggttttgcc ttgcaattct cgtcccacat aagcacttct tttgttttct gcctgttcta
    12361 aaatattagg acctgttagt gtttgtacca tacgacttgc ttcgattatc ggacgggaag
    12421 ataagagcaa acagcacaaa acaagctctt ttactgcatt agcatttaca cctggtgtat
    12481 tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt gatcgtgttc accccaacac
    12541 cagctcttga aattgctagc aaatctggtg taagccattc atctttcact ttcgtactac
    12601 gaataactat tccttcggct ggcaaatcat ctattatttt gaaatattcc ccttgtaact
    12661 tttctatctc ttgttctgga atagcatcaa tcaaatgaac gtgatacata aaaccccctc
    12721 cttactaaca agtatctcga aatttctgca attagacaac tgtttaccat ttgacttcct
    12781 agaagcaaaa agagcagtta gagcagagta ggctgagtga taaaaataag gctatgacaa
    12841 gttactgcca taagccttat tttcgagagc aaaatattga gggtgagata tcttgataaa
    12901 tggtgttccg ttagctgaca ttgataataa gactaaattt caaaaaagtt gaaaagcaaa
    12961 tttcgtcaat ctcttcttat ttcttaaaac taagtggctt tttcacaacc tctttttatc
    13021 taataaccac tgtttccaac tcaactaatt ttgcccacgt ttcaatttga tctgttgtta
    13081 gatttagcga gacaacggta tgatgaccgc cacctgcctg aatccatgaa tggacacctt
    13141 catggaaatt cggtttagtt ttccataaca cacgtgcaac aggtaaatta ggtgcttcag
    13201 tcgttggttc aaaggcttct acttcattga tcagcagttt ataatgcgta ccgaaatctg
    13261 ccatcgatac gactacccct tctcctgctt tcccatcaaa tacaagacgc gccggatctt
    13321 ctctatctcc cattgataaa ggagaaacaa cgattcttgg tttcgtcgca gcaagcgtcg
    13381 gatctacttc catcatatgg gattccaaga ttgcttcttg gccactagct agctcgtaag
    13441 tataatcttc catgaatcct gtatctttcc ctcgagccat gattttcagc aaccgatcaa
    13501 tcgctgcagt tttccagtcg ccttccccag caaaaccgta tccttctgcc atcaaacgtt
    13561 gtacagctag tccaggtagt tgtttcattc catacaggtc ttcaaaatta gtcgtaaaag
    13621 cgttatagcc tccttcttct aagaaacggc gaataccgat ttcctgctga gcttgtactt
    13681 ttacatgttc ctcccatgtc ttttcttcat aatcaccata gtcgaaggta tacaattcct
    13741 tgtactcttc aaataattga tcgacttctt cgtctgatac gcgatcgatt acttgtacaa
    13801 gatctccgat accaaaataa tcaacagtcc aaccaaattg aatctgtgct tccactttgt
    13861 ctccttctgt gacacccacg tttctcatgt tgtcaccaaa acgagccact tttattccaa
    13921 aattatatgg gattttagat tatcttttta gaagttcttg aatgtcggta tagcccgtgt
    13981 ttccgggctt ttccggcatt tttgatattg ttagatactt acaaatgttc ttattacctc
    14041 tcaccatttc cccctcaaaa gtagtaaatg gagtagtaaa accggccttg tgtcaggctg
    14101 ccagccgttc catttctgcc cttgcggaat cgaatgtggc gtgtgcataa tagttcagcg
    14161 tcatggtgat attggaatgt cccataatgt actgcaaggc tttggggttc attcccgcat
    14221 tggctaaccg ggtgcagaat gtatgacgca tagcatgggg cgtcgtcgtt ttcggcaacg
    14281 cttcttcatg gctcttgttg tacttcttca caagcctgcc aaacatcccg ccataatcta
    14341 ccgccgtcat gggatagccg ttctgtttga gaaacaggaa attggcatac ccgtcaataa
    14401 tgaacggctt tgcacctttg cggtttttta tcactcgctg gaacgcctga tacacttcct
    14461 cattcatggg gatttttctg acgccgcttt gggtcttggg ttcgtcaata tagtaaccga
    14521 cgccggtatt tctcaaaagc tggtggctca cgatgattac ccggttttca aaatccaaat
    14581 ctttgtccgt cagtccgcac agttccgaaa tgcgaagccc tgtccccagc agaataacga
    14641 ttgcgtcata atgcttttgg taaaccttat caccttgaat aaaggacaga aaactttctt
    14701 cctgtgctgg tgtaagaggc acctttggtt ccgtatcgtc gtccagaacg tcactaagct
    14761 ggaagtcaaa cgggtttttt cggatgcaat cgtcctgtat ggcggtgtaa aaagccgctt
    14821 tcagggaacg cttgtcgtta ttgatggtct tataagaaag gcccttttct ttcatccgaa
    14881 gcgcccattc ctttgcgtcg gacagcttca cactgtcaat cgggcagctc cccagccgat
    14941 cttgttccag aatacgcatc agccgttccc ggcccagttt ggtgttgtgc ttcacattgc
    15001 cccggtgcct gatctgcttg gcgtagagct ggcagacggt cattttcttg ccgatggtgt
    15061 ctatcccgtc gtcaaggtct ttctggattt ctttctcctt ttcccggagt gaaatatcct
    15121 cccgtttacc tgccggggtc ttatccgtag gcactaactt ccatgagtaa acaaactgcg
    15181 gttttccaaa ggtatcaaca tatttgtaag catatcgtcc gtcttttctc tggctctctc
    15241 cagaccgcaa aacgcgattt ttgctgtctc gtcttttttc tgacatctcg tcatgctcct
    15301 ttccatgacg gaaagagccg catatatgct tataaattat tataacatat ctgcggccct
    15361 ttttcactat tagattgtgt ccaaagtgtc aattattttc tcaaactgct tgcgtttgat
    15421 ctggatgcgg ttgccattca taatgaccca gccagaggcc ggattttcct cggccagctt
    15481 acgcaatttg ttttctccga tacggaaata tttagacgct tcctcaatcg tcagcgtgta
    15541 tttttcccaa ataggcacat catggttcat aatgcagccc cctttcacaa attcaaaggt
    15601 ttgtgtgatt taggggcata gacggacggc tgctggcaca gctccacggg actttcaccc
    15661 ccgcgtggtt ctcacgcggc cctacccatt gcctgcgacg cttttaacgc tcggactgtg
    15721 gctataaggg agtatcatag tcctgcctgt acgtcgtcgc ccgcaagccg ccacgctcac
    15781 attgcggcgt ggtgttcctc gctccgcttt tcccgtaggg aaaagtatca tggcgcacca
    15841 gccgtccggc tcggcacaga cagaatgtgt ccgtatgccc tatgatgcgc cgccgttatc
    15901 ttgcgggcat atttatcaag gtgcgatcag gccaccggga aaacaccgaa acggggaaag
    15961 ggtaagccgt ttcatgctgc cggggccggt tcgtcagttc gtgacgatag cgtggatgag
    16021 cttgatttcc agacggcgct tcatggcctc gtccagacac acatgcggat agccgttttc
    16081 gtcatagagc gtccgggtac acagcttgtt catgtaaccg ctgtaatact gcaaaacgcg
    16141 ctctacggct ccggcatccc cagcgcgggc ggcctcgatc accgaaaggg gcaaaagctc
    16201 aatgcccttg tagttcgggt tcttcatccg cgtctaacct cccttcggca tcaacgcctt
    16261 tagctgtttc cgcagctccg ataaggtatt tgtcctgtgc cgctgaacgg cactccggga
    16321 cattcccaca aggccgccga tctcaccatc tgccatatcc agaacacagt gcaggattaa
    16381 aatactctgc tccatgctgg gcagcgcggc aaaggcttcg gcaacaagct cattgttgat
    16441 atgtaggtca tagccatgcg acgaaaacgt aaagctgtcg ctggggtagt gatctactgt
    16501 acagagcttg tccaaatctg cctgtgacaa agagcttagt gacacttcac ggtcacggcg
    16561 gcgcttcatt tcagccaagt agcttttggc ctcgttacgc agtaccgtct tgcaaaaagc
    16621 atcgaacatg caccgcgctt catagtcgcg ggggatagca tccatcttct cacccccttt
    16681 ctgtggggga cttaggtggt tctctccctt tccgctaaca agactttgac aaggccgcca
    16741 gctacccgct aaatacctcc tttttgaaaa tttctcaaaa aatttttttg gggacagccg
    16801 ggccgcaaaa gacgacaaaa atcgcccggc aggtcgcaaa cctaccgggc gagaaaatgc
    16861 tttgctatgc tgctgattta catggtatag tacatactca taaccggaat agccctgtg
//
LOCUS       DAZUJO010000057        16870 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000057 DAZUJO010000000
VERSION     DAZUJO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.058"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8OL_001969"
     CDS             <1..1087
                     /locus_tag="ACG8OL_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4817951.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1294..2436)
                     /locus_tag="ACG8OL_001970"
     CDS             complement(1294..2436)
                     /locus_tag="ACG8OL_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4817952.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2441..2626)
                     /locus_tag="ACG8OL_001971"
     CDS             complement(2441..2626)
                     /locus_tag="ACG8OL_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4817953.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2980..3225)
                     /locus_tag="ACG8OL_001972"
     CDS             complement(2980..3225)
                     /locus_tag="ACG8OL_001972"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4817954.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3219..3620)
                     /locus_tag="ACG8OL_001973"
     CDS             complement(3219..3620)
                     /locus_tag="ACG8OL_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4817955.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3807..5615)
                     /gene="uidA"
                     /locus_tag="ACG8OL_001974"
     CDS             complement(3807..5615)
                     /gene="uidA"
                     /locus_tag="ACG8OL_001974"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS4817956.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5638..7404)
                     /locus_tag="ACG8OL_001975"
     CDS             complement(5638..7404)
                     /locus_tag="ACG8OL_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS4817957.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7417..8211)
                     /locus_tag="ACG8OL_001976"
     CDS             complement(7417..8211)
                     /locus_tag="ACG8OL_001976"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4817958.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8221..9597)
                     /locus_tag="ACG8OL_001977"
     CDS             complement(8221..9597)
                     /locus_tag="ACG8OL_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4817959.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9717..10364)
                     /locus_tag="ACG8OL_001978"
     CDS             complement(9717..10364)
                     /locus_tag="ACG8OL_001978"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFS4817960.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10448..11875)
                     /gene="uxaC"
                     /locus_tag="ACG8OL_001979"
     CDS             complement(10448..11875)
                     /gene="uxaC"
                     /locus_tag="ACG8OL_001979"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4817961.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11949..13028)
                     /gene="uxuA"
                     /locus_tag="ACG8OL_001980"
     CDS             complement(11949..13028)
                     /gene="uxuA"
                     /locus_tag="ACG8OL_001980"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS4817962.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPACEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13031..14662)
                     /locus_tag="ACG8OL_001981"
     CDS             complement(13031..14662)
                     /locus_tag="ACG8OL_001981"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS4817963.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14966..15637
                     /locus_tag="ACG8OL_001982"
     CDS             14966..15637
                     /locus_tag="ACG8OL_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4817964.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(15797..15988)
                     /locus_tag="ACG8OL_001983"
                     /pseudo
     CDS             complement(15797..15988)
                     /locus_tag="ACG8OL_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(16356..16697)
                     /locus_tag="ACG8OL_001984"
     CDS             complement(16356..16697)
                     /locus_tag="ACG8OL_001984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817965.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT         5507 a         3253 c         2242 g         5868 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa tccatgtaaa catggtttat
     1141 tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc
     1201 gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta
     1261 tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt
     1321 tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat
     1381 agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa
     1441 ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc
     1501 tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa
     1561 aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc
     1621 aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa
     1681 ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat
     1741 taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac ctgtaacaaa
     1801 aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat aaacactatt
     1861 atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct gagtttttat
     1921 tttacctata gtatctacag gattttcttc aataatttca agtacaattg cccttttaaa
     1981 aactactctc aacttattga atatctgttt aatataattt ggtgataatg gttgctcaat
     2041 gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta tatgaattgg
     2101 tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta aaatatgatc
     2161 tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa aatactctcg
     2221 ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag aaaaactttg
     2281 ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc ttctggtcac
     2341 ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc aagtgcctct
     2401 ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc caagaaatat
     2461 ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta cgataaaaaa
     2521 agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta gcatagtact
     2581 gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata gcaccttcct
     2641 tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg aatttcacct
     2701 ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta aatcatcgct
     2761 ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac agcaagaaat
     2821 catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat ctatttaatt
     2881 ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac tattagaaga
     2941 ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt taaattttaa
     3001 gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta cataggtaac
     3061 gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat aagaatgata
     3121 atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata agataatgga
     3181 tagtggaact aataggtact gccgcttttt agatttatct accataggtc ataccccatt
     3241 ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct catattgaga
     3301 attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat aatctttctt
     3361 cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat taatatttcc
     3421 attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata cctgccttca
     3481 tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt tcgaatagac
     3541 ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg tatagatatg
     3601 tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac taatacaaca
     3661 atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata tttatcattc
     3721 tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg attaggctag gctaatcgtt
     3781 ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg tattgatgac
     3841 catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt aaagattcct
     3901 ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc aaaattccac
     3961 acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc ttcgtaataa
     4021 gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc agctactgta
     4081 tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat ccatccatct
     4141 agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac ataccaacca
     4201 taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac atctggcgtt
     4261 gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc tttagttaat
     4321 tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg ttcatttgca
     4381 atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc cttgatcacc
     4441 gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc cacattatca
     4501 tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc atcaataact
     4561 acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata atgtgatgtt
     4621 ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt tacagcctca
     4681 ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt aaaataaaaa
     4741 ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat accaaattct
     4801 tctgtatagg tatcgatcac tttattatca tcaattaaac taattttcat actatataaa
     4861 taagcgtcta gaggttccca aaaaattgga ttcttaagct gaatcattcc tttctttcct
     4921 tcagcttcag ctacaagttg agcctcttcg tcatatatcg ctattaagat ttttccggtc
     4981 cattctgtat cgatattgaa ttctacactt gcatccaaat cagttattct tgtattaata
     5041 gtaatatcac ttatatagtc tttcggagtt gtatataact taacaggtct atgaatacct
     5101 gcataattga agaaatcaaa attaactaag ttttcctttt ttatatcccc attttcctgt
     5161 actgtttcct tcataatccc cacaggtaaa gtactatcgt caataatatt gtttacagca
     5221 actgtaattc gattattccc gtcttttaca aaccgattaa tttcagactc aaatggtgta
     5281 aacccacctt tgtgttgagt aataaacttt ccattcaaaa aaacttttgc ttcatgtgtt
     5341 gcagatccaa aacgaagaac taatctttct tctaataatc ttttaggaag agtcacttct
     5401 ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta
     5461 taagaagagg gaactgaaat cggataactc tctttttcat ttattggttc taaagcaagt
     5521 tcttcctgta agtcatcatt tgtatctaat ttaaatctcc agagaccgct taagtcaatt
     5581 acttgcctcg tctctgtcat aatgggatac aacatataaa ccactcctat ctaaatatta
     5641 aataaatgta tcctttaaac cacaataagc atctattgga cttactccct taaattcaga
     5701 aagacccatt agtttgtcca ccaatatttc taaagtttct tgttttccat cataagtatt
     5761 aatataagtc tttacttgag gtacatcagc caaatgaaac ggacactgta cagatacaaa
     5821 aattgttgga atttcatgaa tgtaaaacgg tatatccgga gttcctttac ttgctggcca
     5881 gacaattcgt tgaactgtgc ctggattaac attagctata ttaatgatca agtcatattt
     5941 tttagttaaa tttttaattg gtctcttagc agcatatatt ccagctatat ttttaatttt
     6001 ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa atgtattttt caacttcaaa
     6061 tccttttgct gacaaaatct gtttcaataa atcaatggct tgaggtctat ttccagcaac
     6121 tacttcacca aacccacctt ttaccccatg tacatctact agtaaaatcc ttttatattt
     6181 ttctggtaca ataggccaaa tatcttgttt atctttaact aatgtaattg cttggtcagc
     6241 cacctcttta aaaattgatt tattttcaat taaaccaatt ttttccagag cttcttgctt
     6301 aggtaataaa atttgttctt ttgctttttt gtgtaatccc aatttagctt ttaaccctaa
     6361 aattcttttt aaagcatcta ataaccgatc atcagtgata attcctttac gatagccttc
     6421 catcatataa ttaaagtctt catctggatc attaaaaaat agaaatagat cacatccagc
     6481 tgcaatagaa gtgggcaata attctgatcg tttcattgcc ccagtcattc ctaacatatg
     6541 actagcatca gtaactacta atccattaaa tccgagtttt cctctaagta agtcagtgat
     6601 taattctttt gataaagttg caggaagtaa atcttcatct ttcagatcag gattaaaatg
     6661 ttttgaataa gctggcatat ggatatgacc tgccataatt gaaggtaatc cagcttctat
     6721 taagcctgag tatacttttc caaaagaatt atcccattct tccgtaccta aactattaat
     6781 actaaaagat agatgctgat ctcgttcgtc aataccgtca ccagggaaat gtttagctgc
     6841 aggcaaaatt ccactctcct gaataccttt catataagct aaactaagtt ctaaaacttt
     6901 atcagggttt tgtccaaaag atcgactaga aatgatagga tttctccaat ttctattaat
     6961 atctacaata ggagcaaaac tccagttaca tccaatagcc gatgcttcaa tcccagaaac
     7021 tctccctaat tcataagcat atttcgcatc atttgttgca ccaattttta cttctacacc
     7081 aacttcagta ccatctgtac aagctccgtt accacctgct tccgtattag cagcaattaa
     7141 tagaggaatt ttactttctg tttgtaagat atagttttga tcatatacct catcagcagt
     7201 tcctggatta tatcgtacag ctccaatatg gtactgattg accatattac tcaagtaatt
     7261 tttatcacga ctagatccca tattaacaaa caattgacct attttttctt ccaaagtcat
     7321 agaatcaatc gtctcttcaa tccaagaaat agcatcttca tctaaattat atggtttttt
     7381 tcgtaaatct acttttatag tcattttctt tctcctctac ttattagtaa gatttttcca
     7441 aaaagtatct atatattttt gttcaatatc tacataatct cccgataaac atttaaataa
     7501 aacatcagtt cttaagtttt cccatgattc tttttcttta ttgaccaaaa taggataaaa
     7561 gaaaacttta tttttatttg cagcttctag atcaccagga gaatcaccaa tcattaatat
     7621 cttatccaaa tcacaaccat tttcaattac acttcgaata atatcttctt tttttccttt
     7681 atcttgacaa cacagttcat caactaatga taataattca tgtctttccc attcttcttg
     7741 aacagcttcc ttattagctg agcttacaat aattatcttc ccatattttt tcagagcttt
     7801 tatagtatct ctagcactta aaaagggttt atctaatcct tttgcttgac taatctgttg
     7861 attgacttct tccgaccatt ttaaagcttt taataaatcc tctgactgag aatattctat
     7921 ttccctcttc aaagatggat tagacaattc ttttgttaga gatacccagt gctttaatcg
     7981 attaatcgat gttacatcat ttttttctaa tgttttaact aaacctaaaa atctgtttat
     8041 tccacgagtc tctgaatata aattaatatt attccaatcc tgtaggaatt tttctctatg
     8101 atgaacatca aaaatctctg cagcaattgg tccgaaaaag agattgtgtt tataagtcat
     8161 cgtatccata acacagccat ctgaatcaat acaaaatatc caattattca tatttctctc
     8221 ctatttatgt ttttgtttta aaagctcgca attttctaag actttatctt tagataatgg
     8281 ataaaggaaa aataaaatta atcctacaat cagaaaacaa attgatggaa ttaatgttga
     8341 agcgttatat aacttttcta aaacatctcc cgtttgtaca tttgctaaag agtcatagcc
     8401 tataatagaa agagtccaac ctccgacgcc tccagctaga gcttgaccta tttttctagc
     8461 aaaagaatat accgcatata cggtcccatc ttctctttga tcagacttta cttcttgatc
     8521 atcaataaca tcagtaatat ttgcccaaat aacagtatta aacacaccca aacccatata
     8581 gccaaccgaa ctaagaatta agaatacata catattacta gcttttatca aaaataagat
     8641 caaatatact attgctgaaa agaacattcc tactgatgcc agttcttttt ttccatattt
     8701 tttacttaaa aacaaagtta aaggcgaaac aactattaaa accaaaatag gattgataaa
     8761 attcattaaa actattccct gtgcatcatt atagtaatca ggaaatacat aattattcat
     8821 actcataatt aataattgag caaggagtaa gaaaatagcc gcaccaatta tagaaagtaa
     8881 tgccctattt ttaactaacc cttttaaaga ttttaacaat gaaactttgt attgagattg
     8941 gtcaattctt actcgttcag tagttagttt ataacatatt aaataacaaa taagtgcaca
     9001 tatcgaaaat attcccgcaa caattgtaaa agttgatcca ccacgaacta tttgagcgcc
     9061 tgaatcatct cttgtataaa taaataatgg tgttaataca ccaatgacca aacttgctaa
     9121 agtagctcca acatttcgga atgtcgatag cattgttctt tcttctgtaa tagaagtaat
     9181 agctgatgcc attgatccat aaggaatatt tatagatgtg taaaaaatac tcccccataa
     9241 tatatacgta acatacatgt atactacctt aacgctcata gaagctgaaa tcatagaagt
     9301 ttgatacatt aaaaaacttg ctaaagcgac tggaccactc attcttaaaa tccacggttt
     9361 aaacttaccc tgcttagttg ctttcgatct atcaacaata cgccccatag ttacatcagt
     9421 aactgcatcg ataaatcttg ctactaaaaa taatgttcca acggcagctc cgcttatccc
     9481 caatactttt gtataaaaga tcattaaaaa agaactagca aaaatgaaag taaaatcgtt
     9541 tccaaaatct ccaaacatat atcctatttt gtctcttata ccaaattttc tttccataca
     9601 attctctccc ttctgttgtt aagcgttttc attctgatga aagtacgtac tatactctga
     9661 agcgtaaaga gtcagtattt attttttaca tttcagagta ttatttgttt tttctcctaa
     9721 taattcttta tctcataaaa cttatctata tatttactag ctactcgact tacttcttca
     9781 aaatcacctt tttcaacaga agccaataaa ttcccaccta caccaactgc aacaactcct
     9841 acttcaaacc aatcttctaa gttctctata cttactccgc cagtaggcat tatatttacg
     9901 tgtggtaatg gagcttgata ggcttttaca atggatggac caaaagcaga acctgggaat
     9961 agtttaatga tatctacgcc agcagtaagt gcctccttta tctcagtaat tgtcatacaa
    10021 cctggtaaat aaggaatttg atataaatta caaatctcag ctgtttgtcg atcaaaagat
    10081 ggactaacga tatattctgc cccagccata attgcgatac gagcagtaaa cgcatctaaa
    10141 accgtaccgg ctccgattat tacatctttt tgatcagcat attttttagc aatttctttg
    10201 attatttctt cagcattagg aactgtaaaa gttagttcaa tccctttaat tccacccgca
    10261 ataattgaat ttattgcttt aatggcctca tccttatttt tcccacgtac aaccgcaatg
    10321 actccagccg cttctaactt acttaaaatt tctacttttt tcatatctat ctccttcctt
    10381 tatggataca tactttgtct agctgtacta acttacaaag aataaattta agcttttatt
    10441 tcaagtatta ttttctactt ccgatttcaa aaaaattaaa ataattatac gcattacgat
    10501 aacaaatatt ctccactatc tgactaagat acatttcatc atctggaatt cggcctgctt
    10561 taacccactc gcttagtaat tgacataata ctcgacgaaa atactcatgg cgaggatagc
    10621 ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgtg
    10681 ccatcgtagt caactgttga cgcattcctt cagctgtatc attgaaccac caagcacatc
    10741 ccaattggat tttttgaatc ataccccctt ggaagctttg cattgctgtt gctagtggaa
    10801 gccaatcagt tggatttaat gaatataaaa ttgtttttgg taagcaccca ctcttttcag
    10861 cggtatcgaa caatgctaca acattttgtg tcaaactagc ttgatccccc atcgaatcac
    10921 caccgacatc tactccaatt tgttccttta atatacttga attatttctt aaagcattta
    10981 aatgcatttg catcgtccaa ccatatcttg tatactcaga catcaaatgt aattgaatac
    11041 ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct aaagctttta
    11101 ttaacagatt gtcgagtatt ttttcatctg ctatttcata aaaataagta ttcaaaccgt
    11161 gatctgctaa tcgacagcca atttgatgaa aatgatccac tcgttggctc aatgcttgct
    11221 tcaacccatt aaagtctttg attggtatag ccgctgtttg actaagtttt aaaatatagt
    11281 tcgaaaagcc tgatgatttg atactccaga ccttatctgg acggaaagta ggtaatacct
    11341 tgaaagaatt atcctgttta gaaagctcag tatgatacca caagtcatct gtaggatcat
    11401 ccgttgtaca gatggctttc acatccatca tctccaacaa ttctttcggg cgataggaat
    11461 ccgtttgaat tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg
    11521 tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa ataggattac
    11581 ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt ttcataatca tcacctggac
    11641 cactgatcaa ctcttcttct ataccattag ctcgcatcaa tcgccattta taatgatctc
    11701 cagctccttt atcatttaac catacctgag tcaaattctt gtattgctta ttttctagca
    11761 aatatgctgg gtctaaatga caatgatagt cgataattgg ttggtgtttt gcaaatttat
    11821 gaaaaagtct tttagctatt tcattctcta ataaaaagtt cttatctaaa aacatattta
    11881 aacactcctt tccttccgtt agtttttaga agagaaaatt tttcctctaa ataaactccc
    11941 ttatataatt atttactcgc ccccttttca agcgcttccc aaataccatt taaataaact
    12001 gcacctagag ctcggtcata taatccataa ccaggtctac cggtttctcc ccaaatcatc
    12061 cgaccatgat ctggacgaat atagccatta aatccattat cgtacattgt ttttaatatc
    12121 ttatacatat ctagagaacc atattctgat ttatgcggcg cttcgtaaaa atcgagtcca
    12181 ttgtcactta aatatttgat attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa
    12241 aattcagata gaatttcata gacatcatta tctggtcgtt ctgcaatact tccagtacat
    12301 aatgtaatac cattagaagg agaatccact acattacaca accaatctaa atcgtcacga
    12361 tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg gtgaactgcc
    12421 attttaatcc caacttcttc acatgccggg ataattgctt tgagaaaata tgcaaagttc
    12481 tctcgtaatt tctcttcatc aacttcttta taggcttcta ataattgctt aatttgtgat
    12541 aaccgatgag gttcccaacc cggcaattca aaattattag tagaattttc tacttctcta
    12601 ataatttcct ccggatcagt agggatattt tttgaaatat aggctaacgt agaagaccca
    12661 tcatctaaac gataatccaa atccgatttt acccaatcaa atactggcat aaaattataa
    12721 cagataacct caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata attctcaatg
    12781 tattgatctc gtgttggtaa accaattttg atatcatcat gaacattaac actctcaata
    12841 actttcaagg taagtccatt ggcttcgact tcatctttta aagatttaat tcgttctctc
    12901 ggccaaacct ctccggctgg tatatccatt aatgttccaa caattccttt cattgctggg
    12961 atctgacgaa tttcttgtag tgagattttt tcttcatttg ccccatacca tctaaaagtc
    13021 atttccatag ttactttcct ccaacttctg ttatatattt attgattgtt ttacgaactg
    13081 cttgaggacc tactagcatt tcgcaaaaga ttttttcagt tttctttcct accccaactt
    13141 gatataaatc taaaccaaag atttctttat tttctaaaat ttcctgtacc gcacaatgaa
    13201 tctttgaagg atcttgttca cctaatttca atactgataa tttatcctgc aactctacca
    13261 ataacggatc aggacttggc acaaaatttt ccccttgatc atcgactgcc agtaaataac
    13321 gtaaccaagc agcaatagcg ataggaataa atgttagatt ttctgcattt ccgtcttttt
    13381 cgacatactt tttaattgtt tcaccatagc gaatcgctac tttttgagaa gtatctgttg
    13441 caattcgttg tggtgtatct ggaatatagg gattaggcaa acgtttatta attacttcat
    13501 caataaattg ttttggatca ataatgcctg gattttcaac tactggtaaa ccttctaaat
    13561 aacctattcc tttgattaaa ccaaccaaat catcatcctg catctctgct gaaattgatt
    13621 gatatcctag cagacaacct gttactgcca aagcagtatg aagtggattc aagcttgtcg
    13681 taaccttcat gatgtctgct ttatcaaccg tctctctatc tgtcataatc acaccagcct
    13741 tttccaattt aggccggcca tttggaaaac tatcttcaat aactaaataa tgaactacct
    13801 cagtattagt gaaagcagca atattggtat gtttttttgt gtgaatgata tctaaatcct
    13861 cgagccccat catcaataga tcttccttta cattctctga aggatttggc gtaattcgat
    13921 caatcatact ccaaggaaaa gtcacttttt ctttatcctt taaataagca ataaattttt
    13981 cagaaacata tccttctttt tgccaagctt ctgcaatcgt aaaaatacta tcttgaaaac
    14041 gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac tctccagcat
    14101 taaatctgct atataacaag gatgttacaa tactcatcgt atgttgagga ttgctaggtc
    14161 cagatttgat atcagctttg actaccgaaa agaattgccc tttagaatct tttaaactgt
    14221 aacctttttc agtaatagta aacgttgtaa actgtaattt aggatcttca aaatatttaa
    14281 ccactctttg atagtccgct atattcttcg gattgcaata taaactatca gccgttactg
    14341 ccaaaagtct ttttttgagt tcaccatctc cactcataac aatttccaaa atgtcattgt
    14401 tatagtcatg ataagctttt tccactactt cttcatcaaa tgtttcacac acaactactc
    14461 ctgtttctaa ttcattttga tctgctaagc attgagcaat ttctgcatga aatcctcgat
    14521 aaagatttcc tccaccaaaa tgaagccata ctggatgttg ctggccttct tgcttaactt
    14581 ctttgacatc aaaacacggc agtagaatct gcatttcatt gaattcttcg atagaatttt
    14641 gccaattttt aatcgaaacc ataattaacc tcctcaaact gatatgttag ttttgataca
    14701 ttttatattt taaaacccat tgtaaccgtt gtcaatataa aaagggaata aacttgcttt
    14761 tttctaccat atcagaatga ttgtattttt tattcagaaa aataaaatac tccttctttt
    14821 tggactgatt ttatcgaaac ttattttttc tttttttcat gtattctttt atttttataa
    14881 ctagtgtatt acttcaaaat ttgatattat atggcatgaa cagaattaaa atatcagtta
    14941 aaataaaagt taaggagcat tgtgtatgac aattaaaact agtctccaaa atcaagctta
    15001 ccaatctatt cgccgacaaa ttatctatgc tgacttagaa cctggaaaaa aagtatcaga
    15061 aaaaaaatta gaagaaacac tttctattgg tcgtacacca atacgtgaag cactgattca
    15121 actccgacaa caaggtcttg ttgatacaat tcctcaaagc ggaaattata tctcattaat
    15181 tgatttagat tcggccaaaa atgctcgatt tgtcagagaa catttggaac gacaaattat
    15241 gttagagtgt tgtgccaaaa tgaataacca aaaaaaacgg attttagaaa ctataatttc
    15301 agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt ttttttcata atgataattt
    15361 attccataaa gcttgttttg aaattgcaga gcgagaagaa gtatggcaat ggttagaaaa
    15421 taacaataca cacttagaac gatttcgatg gctacgagta aaaacggaag gattaaagtg
    15481 ggaaaaaatt atggaacaac attatcaact atttgaggca ttaacaaaac aagatccaga
    15541 agaagctaac tttctaacat cgttgcatct tcatttaatg cttaatgagc aaaatattgt
    15601 tttagctact catccaaaat atttcaaaaa tatataactt ttaactcttt ttaaattaaa
    15661 tggttgttct gatcagaaaa atatcgcttg ttttcttatt atgtaatata tttcataaac
    15721 tattctgcat aacaactatt gtttgtactt tttacattgg ataactaatt atgactgctg
    15781 ataacttttg atattaaact agtatctcca atacaaataa aaaatagata tcttgttcat
    15841 caaaattagt tcgtggatac ttaccttcat ttacatcatt cagcggtttc ccattttctt
    15901 ttattctatt atttcatttt ctaatagact tggcttcatt tcgcaatact ttttttcaat
    15961 aggcatcaaa ttggtgttgt ttagatattt agttgtagtt ttgtgacatg ataagtatgc
    16021 tcctttttct cttttccact aatacaacga tcaaagggtg cttttttcaa aaaactagaa
    16081 aaatcaaaat aagattcgta ttttgattct atatatcctg tttcaactac atcttatatt
    16141 tcacaataga ataaatagcc ttttgcatag ctgcttaact tataagaaat tgcaatttac
    16201 ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat tcttcttaca gataattaaa
    16261 aatagcgcgt aacaaaaatc ggcattcaat cacataatta ggattgatgc cgattactta
    16321 tattctacgt atagttttag tattcttatt ttttatcacc agtaaccaat tgagaacagc
    16381 cctaaagcag aatagtcttt tatctcaaaa cgcttaattt caggcttagg aatatgaata
    16441 atcttttgag tatattcttt ctcttttaat cttactgaag aaataccact gcctatcttt
    16501 tttacaaata tttcatttgg tgtacagact aataaaacaa tatttgtaat caatccttgc
    16561 actaaagaat taaaaggaag actatttaaa acagggtgtg cataactata acatgtgaag
    16621 tagttatttt gaactttaac ttttaaaaac tttaaaaata atcttatttg ttcttctaaa
    16681 caatcatcta tactcatata atcactccac taattaatct ccccaaaaag acacagcatt
    16741 aagagatata ccaacacaac agtgcaaata actctacatc ttccttcgca accttatttc
    16801 aataaaccat aaaaattatt ttttaataga aactgaaaat taaaactaga aaaagtagtt
    16861 ttaccgaatg
//
LOCUS       DAZUJO010000058        16025 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000058 DAZUJO010000000
VERSION     DAZUJO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.059"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8OL_001985"
     CDS             226..894
                     /locus_tag="ACG8OL_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS4817966.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8OL_001986"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8OL_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817967.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8OL_001987"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8OL_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4817968.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8OL_001988"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8OL_001988"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS4817969.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8OL_001989"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8OL_001989"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4817970.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="ACG8OL_001990"
     CDS             complement(5260..6684)
                     /locus_tag="ACG8OL_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS4817971.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="ACG8OL_001991"
     CDS             6859..7764
                     /locus_tag="ACG8OL_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS4817972.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8OL_001992"
     CDS             7855..8532
                     /locus_tag="ACG8OL_001992"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS4817973.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8OL_001993"
     CDS             8529..8699
                     /locus_tag="ACG8OL_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817974.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8OL_001994"
     CDS             8813..9709
                     /locus_tag="ACG8OL_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4817975.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8OL_001995"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8OL_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4817976.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8OL_001996"
     CDS             11372..12118
                     /locus_tag="ACG8OL_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4817977.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8OL_001997"
     CDS             12115..12954
                     /locus_tag="ACG8OL_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS4817978.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG8OL_001998"
     CDS             12955..13905
                     /locus_tag="ACG8OL_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS4817979.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG8OL_001999"
     CDS             14046..14798
                     /locus_tag="ACG8OL_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817980.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG8OL_002000"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG8OL_002000"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS4817981.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5201 a         3000 c         3228 g         4596 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actgg
//
LOCUS       DAZUJO010000059        15860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000059 DAZUJO010000000
VERSION     DAZUJO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.060"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACG8OL_002001"
     CDS             complement(12..482)
                     /locus_tag="ACG8OL_002001"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS4817982.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACG8OL_002002"
     CDS             complement(641..1099)
                     /locus_tag="ACG8OL_002002"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS4817983.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACG8OL_002003"
     CDS             complement(1270..2415)
                     /locus_tag="ACG8OL_002003"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS4817984.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACG8OL_002004"
     CDS             complement(2428..3345)
                     /locus_tag="ACG8OL_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS4817985.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACG8OL_002005"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACG8OL_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS4817986.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="ACG8OL_002006"
     CDS             4437..4607
                     /locus_tag="ACG8OL_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4817987.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="ACG8OL_002007"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="ACG8OL_002007"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS4817988.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="ACG8OL_002008"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="ACG8OL_002008"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS4817989.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /gene="dapA"
                     /locus_tag="ACG8OL_002009"
     CDS             6871..7752
                     /gene="dapA"
                     /locus_tag="ACG8OL_002009"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS4817990.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /gene="dapB"
                     /locus_tag="ACG8OL_002010"
     CDS             7749..8465
                     /gene="dapB"
                     /locus_tag="ACG8OL_002010"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS4817991.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="ACG8OL_002011"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="ACG8OL_002011"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS4817992.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="ACG8OL_002012"
     CDS             9182..10183
                     /locus_tag="ACG8OL_002012"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS4817993.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="ACG8OL_002013"
     CDS             10180..11541
                     /locus_tag="ACG8OL_002013"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS4817994.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="ACG8OL_002014"
     CDS             11501..12652
                     /locus_tag="ACG8OL_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4817995.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="ACG8OL_002015"
     CDS             12627..13796
                     /locus_tag="ACG8OL_002015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4817996.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="ACG8OL_002016"
     CDS             13971..15350
                     /locus_tag="ACG8OL_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4817997.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15631..15860
                     /locus_tag="ACG8OL_002017"
                     /pseudo
     CDS             15631..15860
                     /locus_tag="ACG8OL_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5185 a         2799 c         3378 g         4498 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa attcgggaga agcacaagga ttatggctgt
    15721 ttacggatga ccaatgaact aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag
    15781 cgcttgatca agaagctagg aataaaagta acggcataca cgcgtaaatc acgtcgctac
    15841 aattcttatc gaggaaaagt
//
LOCUS       DAZUJO010000060        15255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000060 DAZUJO010000000
VERSION     DAZUJO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.061"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACG8OL_002018"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACG8OL_002018"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS4817998.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG8OL_002019"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG8OL_002019"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4817999.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACG8OL_002020"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACG8OL_002020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS4818000.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACG8OL_002021"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACG8OL_002021"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS4818001.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACG8OL_002022"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACG8OL_002022"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS4818002.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG8OL_002023"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG8OL_002023"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS4818003.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACG8OL_002024"
     CDS             6726..7466
                     /locus_tag="ACG8OL_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS4818004.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="ACG8OL_002025"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="ACG8OL_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS4818005.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG8OL_002026"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG8OL_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS4818006.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /gene="rpe"
                     /locus_tag="ACG8OL_002027"
     CDS             10609..11259
                     /gene="rpe"
                     /locus_tag="ACG8OL_002027"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS4818007.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="ACG8OL_002028"
     CDS             11260..11898
                     /locus_tag="ACG8OL_002028"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS4818008.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="ACG8OL_002029"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="ACG8OL_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS4818009.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="ACG8OL_002030"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="ACG8OL_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS4818010.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..15249
                     /locus_tag="ACG8OL_002031"
     CDS             13675..15249
                     /locus_tag="ACG8OL_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS4818011.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5114 a         2864 c         3319 g         3958 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca
    14701 gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa cagaagaatc aacaacacct
    14761 gcgcctacaa caccatcaac agatcaaagt gttgatacag gaaacggcac aggaagtagt
    14821 actccggctc caacgccaac accaacacct gaacaaccaa aacctgtaac acctgctcca
    14881 gctccttctg gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa atatattggt
    14941 actccatatg tttggggcgg taaagatcca agtggctttg actgctcagg gttcacacgc
    15001 tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc tcaagaatca
    15061 gctggaacaa aaatttcagt ttctcaagca aaagctggag acttgttatt ctgggggtca
    15121 caaggcggaa cttaccacgt agcgattgct ttaggcggag gacaatatat ccatgctcct
    15181 caaccaggcg aaagcgtaaa agttggttca gttcaatggt ttgcccctga ctttgctgtc
    15241 agcatgtaag aatga
//
LOCUS       DAZUJO010000061        14994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000061 DAZUJO010000000
VERSION     DAZUJO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.062"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8OL_002032"
     CDS             <1..608
                     /locus_tag="ACG8OL_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818012.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ACG8OL_002033"
     CDS             628..1656
                     /locus_tag="ACG8OL_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS4818013.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ACG8OL_002034"
     CDS             1653..2411
                     /locus_tag="ACG8OL_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS4818014.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ACG8OL_002035"
     CDS             complement(2497..3237)
                     /locus_tag="ACG8OL_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS4818015.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ACG8OL_002036"
     CDS             3298..4551
                     /locus_tag="ACG8OL_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS4818016.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4846..5100
                     /locus_tag="ACG8OL_002037"
                     /pseudo
     CDS             4846..5100
                     /locus_tag="ACG8OL_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ACG8OL_002038"
     CDS             5222..5548
                     /locus_tag="ACG8OL_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS4818017.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ACG8OL_002039"
     CDS             5616..6329
                     /locus_tag="ACG8OL_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS4818018.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /gene="licT"
                     /locus_tag="ACG8OL_002040"
                     /pseudo
     CDS             6639..7491
                     /gene="licT"
                     /locus_tag="ACG8OL_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7612..9477
                     /locus_tag="ACG8OL_002041"
     CDS             7612..9477
                     /locus_tag="ACG8OL_002041"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4818019.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9491..10934
                     /locus_tag="ACG8OL_002042"
                     /pseudo
     CDS             9491..10934
                     /locus_tag="ACG8OL_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11042..11743
                     /locus_tag="ACG8OL_002043"
     CDS             11042..11743
                     /locus_tag="ACG8OL_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4818020.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11869..12381
                     /locus_tag="ACG8OL_002044"
                     /pseudo
     CDS             11869..12381
                     /locus_tag="ACG8OL_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12779..12988)
                     /locus_tag="ACG8OL_002045"
     CDS             complement(12779..12988)
                     /locus_tag="ACG8OL_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFS4818021.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13229..13804
                     /locus_tag="ACG8OL_002046"
     CDS             13229..13804
                     /locus_tag="ACG8OL_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS4818022.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13804..14244
                     /locus_tag="ACG8OL_002047"
     CDS             13804..14244
                     /locus_tag="ACG8OL_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS4818023.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14260..14961
                     /locus_tag="ACG8OL_002048"
     CDS             14260..14961
                     /locus_tag="ACG8OL_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS4818024.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5066 a         2485 c         3161 g         4282 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    13141 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    13201 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    13261 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    13321 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    13381 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    13441 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    13501 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    13561 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    13621 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    13681 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    13741 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    13801 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    13861 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    13921 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    13981 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    14041 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    14101 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    14161 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    14221 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    14281 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    14341 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    14401 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    14461 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    14521 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    14581 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    14641 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    14701 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    14761 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    14821 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    14881 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    14941 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa aatt
//
LOCUS       DAZUJO010000062        14990 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000062 DAZUJO010000000
VERSION     DAZUJO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.063"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(501..1067)
                     /locus_tag="ACG8OL_002049"
     CDS             complement(501..1067)
                     /locus_tag="ACG8OL_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4818025.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1067..2023)
                     /locus_tag="ACG8OL_002050"
     CDS             complement(1067..2023)
                     /locus_tag="ACG8OL_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS4818026.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2023..2679)
                     /locus_tag="ACG8OL_002051"
     CDS             complement(2023..2679)
                     /locus_tag="ACG8OL_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS4818027.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3049..5067
                     /locus_tag="ACG8OL_002052"
     CDS             3049..5067
                     /locus_tag="ACG8OL_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4818028.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5137..5343
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5494..7908
                     /gene="leuS"
                     /locus_tag="ACG8OL_002053"
     CDS             5494..7908
                     /gene="leuS"
                     /locus_tag="ACG8OL_002053"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS4818029.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8256..9809
                     /locus_tag="ACG8OL_002054"
     CDS             8256..9809
                     /locus_tag="ACG8OL_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS4818030.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10190..11974)
                     /locus_tag="ACG8OL_002055"
     CDS             complement(10190..11974)
                     /locus_tag="ACG8OL_002055"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818031.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11989..13710)
                     /locus_tag="ACG8OL_002056"
     CDS             complement(11989..13710)
                     /locus_tag="ACG8OL_002056"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818032.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13810..14571
                     /locus_tag="ACG8OL_002057"
     CDS             13810..14571
                     /locus_tag="ACG8OL_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4818033.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4886 a         2748 c         2964 g         4392 t
ORIGIN      
        1 ctgctttgat cataaaagtc tgcatattca tcactgatat tttttctaat aaattcttcc
       61 atcataaaaa tcacctcaaa acgattatac gaacttacgt tcttgttgta aagcgatatt
      121 tgaggggcaa aaaaggggca taatttgact gatattaagt tagctattga tatatgaatg
      181 acatacaaaa atataaaatc ttttaaaaat gcttatatat caatgttttt gccattcttt
      241 tatatatctg ttatatgtca aaaaaatgtg ttcaatttca atttgtattt gaataaagag
      301 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caatcaaaaa cagcttctca
      361 tgtttttgat ttggttggac ttattaccgc agatataagc ttttgagcac cgtgaataaa
      421 gaggttatga aatcaacgct ctaacctgag tattagagaa aaaaacgcaa aacatatcat
      481 ttatgttttg cgttttttat ttatcttttt ttctcaatac aatataaaat aggcggctgg
      541 ttcttttggt tgataaaccg ataattcagc acattatatg tttcctgtgg cagtttggaa
      601 caaaaagaat caaccatatc cagttctttt tctccacctt catgaccata gtagacgaca
      661 aggatcaatc gtccgcgagg cacaagtcgt ttcaatattt cttccatagc tgtgcgtgta
      721 gtctctggca tagtgatgat tgctttatca ctttttggga gataccctag attgaaaatc
      781 cctgctttga gattttgttg ttcagctatc acttctccta acgtttcgtg gccttgtaga
      841 aacaatgaga cacgttcttc taactgacgc tgttctagcc tttcacgagt agcatcgatt
      901 gcttgttgct ggatatcaaa actatagaca tgaccagttt ttccgatgtg ttctgctaga
      961 aaaaccgtat cgtggccatt gcccatcgtt gcatctacga cgtgatcccc tggttgcaag
     1021 atttcttgca gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag acgctcacct
     1081 ttttccgtac atcatatttt ccttggaagc tatctctgcg aagaagttct tcatcaattg
     1141 cattcaagac ttcccatttt ttcaagctcc acattggtcc aatcaaggaa tcccacggcg
     1201 catcccctgt caaacgatgg atgatgatct cttttggtat catttccagc tggtcgcaaa
     1261 tgacattgac atagtcagga cggctcatta attgaaggcg accttcatga tagtcccgca
     1321 acattctcgt tttgcgcatc agatgcatca aatggagctt gattccttgg atatctgaat
     1381 ctaaaatggt gcgtctgaca ttttctttca tcatttctaa actttcgcct ggtaatccgt
     1441 tgatcaaatg cgtacaaaca cggatattat gtttgcgtaa tttagccacg ccatctaaat
     1501 aagtttgata atcatgtgct cggttgatca gttttgaagt ttcttcaaaa gttgtttgca
     1561 atccaagttc tacccataaa tacatccgct cgttcagttc agcaaggtaa tcaactactt
     1621 catctggcag acaatccggt cgtgtaccga ctgataatcc tacgacacct ggtaaattga
     1681 ctacttgctc aaatcgttct ttgattattt caactggcgc atgggtgttt gtaaaatttt
     1741 ggaaataaac gatgtattgt tggacatgag gccatttttg atgcatcatg tcgatttctt
     1801 tctggaattg gattggaaga gggtcttcag gtgcaacgat catgtctccc gaaccagata
     1861 cactgcaaaa agtacaaccg ccatgggcaa cagtcccatc tcgattcgga cagtcaaatc
     1921 ctccatcgat cggcaccttg aaaattttcc cgccaaactc atttcgtaaa gcgtaattcc
     1981 aagaatgata tcttttattt ccttctgaat aaggaaaagt cattaaaagt ccttctttct
     2041 gtaagagtta tctatttgtt ttcttttttc tctatacact ggataagtca ggcacagcca
     2101 agaaagactg agtaagtagc cgccgatgat atcagtagga aaatgaacgc ccagataaat
     2161 gcggcttatc ccgacactta ctataataaa catcatcagc aattgaagca atgtgcgtaa
     2221 ggttcttttt tgaacaaaag ctggaagcag aaatagtaaa gaaccgtaaa gaatcatact
     2281 gcctgctgca tgtccactag ggaaactgta acttgtttca actactaagt gcgccaatga
     2341 aggacgctct cgcatgaaaa atagtttgat caccgggttg atgattccag caattacaat
     2401 aaatccagaa acgaaccaaa atgtttctgc cctttttttc ttataccaca tccataaagc
     2461 cgcacaagca aataaaatga tgacggtaag aggattaccg aatttagtca taaagagttg
     2521 gtagctgttc agatcaggat aagaagctct gacaatgcct gtaaaccact gatcgaaacc
     2581 agaaagccac gacagatgga actttacgat ccagcccaaa atgaaaaaca ggacaagaaa
     2641 gaagctacca gccacctgaa atgatgtttt atatttcata caattcctcc tttgaagcaa
     2701 tgacttatta tagcattttt ggtccagcga aaaaaacggt aaagagataa aaccaatgta
     2761 tgattcctct ttgaataacc aatattctta taaaaaaaat aactgtaatc ggaaagacat
     2821 tttttggtaa tgcaaatgaa atgttaggga aataaaatca gtgtttttcg aaatcctttt
     2881 aaacaagaac gtagacgtag gaagaacttg aaaatgaaca gaagttgttc attacggtaa
     2941 attacaatat gattacaaaa aacaacaaag ctcttaaata acttttaata aatattaaga
     3001 gaagttataa ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt
     3061 gcaagaaaag aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg
     3121 gcagcaatcg tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg
     3181 gttcctgttc aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca
     3241 tttatcacag aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca
     3301 tcagtgatga tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa
     3361 gcaccttatt ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg
     3421 gatacgcttg aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat
     3481 ccttcttttg ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc
     3541 aacggttatt actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca
     3601 gcttgtttga cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc
     3661 atcgcaactt acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga
     3721 gcgactacag gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct
     3781 ggtaatacaa actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct
     3841 gtttggggaa tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac
     3901 atccaaaaca atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat
     3961 ggctcttcga cgaataattc cggtaacaat accaactctt caggaaatgc tggtacatca
     4021 aacggaggac aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag
     4081 atctcaaatg atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac
     4141 aatttcgttt atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga
     4201 gcaaatactg gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct
     4261 tctaatacag cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc
     4321 tcaaataaat acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac
     4381 tttatctacc caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca
     4441 ggtaatacag gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt
     4501 tcgtctcaag gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa
     4561 aacggcatca cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat
     4621 ccaggacagc aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact
     4681 acttctgcag cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact
     4741 ggtaatacga tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat
     4801 catatcacga tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca
     4861 ggacagcaag tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact
     4921 ggttcgaaaa cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat
     4981 ggaattacaa tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca
     5041 ggtcaaacgt tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc
     5101 acaaaaatat ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt
     5161 aaaaagactg aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa
     5221 ctcgcctttg agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta
     5281 caacgttcga ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta
     5341 taaaaagagc aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa
     5401 agcaaaagga aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa
     5461 acaagtgttt tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag
     5521 aaaaaatggc aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa
     5581 aagccaaaat tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta
     5641 gggcatcctg aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga
     5701 tacaacgtgc tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg
     5761 ctagatacag gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc
     5821 caaatcaatt ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg
     5881 gagtattata aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat
     5941 gaagcagaag tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa
     6001 gtgatcgatg ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa
     6061 tggatgctga aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat
     6121 tggccagaaa gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat
     6181 attgaattta aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat
     6241 actctattcg gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt
     6301 acgacccctg aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca
     6361 gatctaaaga gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca
     6421 atcaatccag ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca
     6481 tacggcacag gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct
     6541 caaactttcg gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct
     6601 tacacagaag atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa
     6661 gcaatcgata aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc
     6721 tatcgcttac gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc
     6781 atccattggg aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg
     6841 cctaagacaa aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag
     6901 gagtgggtca atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca
     6961 atgccacaat gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa
     7021 aaagaactag cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt
     7081 ggagcagaac atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac
     7141 gatatcggtg ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc
     7201 ttaggcgaaa acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat
     7261 gtggtcgaaa cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta
     7321 gatgcttcga ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt
     7381 gtatggcgtt tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat
     7441 gatggcaagc ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa
     7501 aatcttcatt tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa
     7561 gtggacgctc tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct
     7621 cctcacatgg gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca
     7681 ccatggccaa catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa
     7741 gtaaacggta aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta
     7801 gaaaaagcag cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga
     7861 aaagttattg cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt
     7921 caataaggac tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc
     7981 tcagattttt tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat
     8041 gttctcaaac acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat
     8101 catcaaattt ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag
     8161 agtacatacc aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac
     8221 gcataatgaa tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg
     8281 aagtattatc agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa
     8341 tgttcaagct tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt
     8401 tcctatatcg ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata
     8461 atttgtatca cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc
     8521 ttctacttat ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc
     8581 aaagagcaca acagttttta cataataaac caacttctca agatgatgac tatctttatt
     8641 ttgagaagac ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc
     8701 tgagatcact tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga
     8761 acacattcca tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc
     8821 ctggtggata cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt
     8881 tagctggaac aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg
     8941 ctgcttctca catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag
     9001 ggttgttagc tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca
     9061 aacttgatac agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat
     9121 ttgtgccgat tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga
     9181 ttcctgaagg ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta
     9241 tctattcttc tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc
     9301 aaacgtctat tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg
     9361 gcaacgatgg tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca
     9421 tttacactac tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc
     9481 agccagtaca aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa
     9541 gtttgtttta tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg
     9601 aaaactatca gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa
     9661 gcgcgaatac gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga
     9721 aaatgtttgc ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc
     9781 aactatcgca agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca
     9841 ccgagtcaca agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc
     9901 gtgattcgga gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt
     9961 acaagatcgt gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg
    10021 gattgtgaca agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag
    10081 tatcattagt attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat
    10141 cgatcatgga ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct
    10201 gaaattgact attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg
    10261 ttcctttttc gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg
    10321 ttgacaaacg gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt
    10381 gctgaatcaa tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga
    10441 caggaggatt cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata
    10501 acgtgccgtt ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt
    10561 gatcacattg tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc
    10621 cgtaggcgat attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc
    10681 caaaagaacg acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga
    10741 tctttccttt attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg
    10801 caccagttgg accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat
    10861 tcatcaatat tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt
    10921 tcggttgttc taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata
    10981 tctcaaatat cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag
    11041 cggtagaaat tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga
    11101 tcgtcacacc acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga
    11161 gttgattaat gaaacgaatg gccggataga tagcacagtt cataaattga gcacgtttaa
    11221 aagcgttgta ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat
    11281 tgaatgtttt gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac
    11341 ccagttcata ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa
    11401 caattgaact gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa
    11461 acagcatcag accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg
    11521 tcaataacac ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta
    11581 cttgatgatt gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc
    11641 gcaccctcaa tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag
    11701 tgatgctgct taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt
    11761 tgatatcact gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat
    11821 cgatcccgat tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc
    11881 atagaaacaa tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca
    11941 aagcacgcgt gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg
    12001 tgattttgca aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg
    12061 tccaacgatt tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag
    12121 gcgttgagca acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa
    12181 tgctgcatct gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc
    12241 tggtttgatc aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga
    12301 accgccttga gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa
    12361 ttgagcgatc ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg
    12421 caagttgctt aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg
    12481 ggagcggatc tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc
    12541 tatcgtaggg tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt
    12601 cccgccaacg atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac
    12661 tggatcagag atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg
    12721 atcaaaggtt ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg
    12781 cagtatttct tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat
    12841 agctgcgatc atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac
    12901 accgatttgt aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata
    12961 aacgattccc atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt
    13021 gatcatttga ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg
    13081 attatcaaag gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat
    13141 ccgatccatt tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata
    13201 aacaatccat acataaaaaa caacagagat caatgtaacc catgttagta atagagaata
    13261 acgaaacatc atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca
    13321 aaataaaaca atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga
    13381 tccagaacta aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt
    13441 ctcccgcgtt tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac
    13501 tgcagcgatt gttcctagaa acgctacaag cagcatttca actccaattt tttttataac
    13561 tgaggcttcg ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc
    13621 taatgtaccg gcgatttcta aacagatcat cacaaaggta aggagagcga gtcctttgtt
    13681 tttctgtaag aatgttttta agatagacat attattcctc cttttttcct gatacttttc
    13741 gattctatac cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg
    13801 tgagaaaaaa tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca
    13861 aaatcaacga tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag
    13921 aatgattatc gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat
    13981 agaaaattag atataccgat caaacagatg aagaatttat atggaaaaac attgacggag
    14041 ttatatgaga cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa
    14101 caaaaacaaa aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac
    14161 gaggaagaat ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa
    14221 gatattgtgg aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg
    14281 ccagcttcca gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta
    14341 tttactcaag acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg
    14401 aaatccgaga tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat
    14461 gaaaaaggat ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa
    14521 aacaatatca gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt
    14581 gtttaagata actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga
    14641 ttacgggttt aatgtggggg attattagtc gtggatagat agaattttag ttttttagag
    14701 gaaatatgtc tttaaaaatg gaatggttgc aattcttagc gatatatacg atgtaattgt
    14761 cctcttaaaa aagtatggaa aaataattgt attgggtgtt atgtataatt catggtgaac
    14821 tgagactaat gagaagaaaa attcatattg ttatgagtga gattgtatca gatgagaatg
    14881 ataaattaaa taggtgcata aaagcattac acttaaaata cattgttgct tacattatta
    14941 caatatgtta ataaggcaaa aataaataga tggattgaat aaacatattt
//
LOCUS       DAZUJO010000063        14975 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000063 DAZUJO010000000
VERSION     DAZUJO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.064"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..632)
                     /locus_tag="ACG8OL_002058"
     CDS             complement(51..632)
                     /locus_tag="ACG8OL_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS4818034.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="ACG8OL_002059"
     CDS             complement(638..1897)
                     /locus_tag="ACG8OL_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS4818035.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="ACG8OL_002060"
     CDS             complement(1899..2945)
                     /locus_tag="ACG8OL_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818036.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="ACG8OL_002061"
     CDS             complement(3180..3455)
                     /locus_tag="ACG8OL_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS4818037.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACG8OL_002062"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="ACG8OL_002062"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS4818038.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACG8OL_002063"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="ACG8OL_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS4818039.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACG8OL_002064"
     CDS             complement(6554..7234)
                     /gene="cmk"
                     /locus_tag="ACG8OL_002064"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS4818040.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7283..7918)
                     /locus_tag="ACG8OL_002065"
     CDS             complement(7283..7918)
                     /locus_tag="ACG8OL_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS4818041.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7984..9387)
                     /locus_tag="ACG8OL_002066"
     CDS             complement(7984..9387)
                     /locus_tag="ACG8OL_002066"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS4818042.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(9374..10405)
                     /locus_tag="ACG8OL_002067"
     CDS             complement(9374..10405)
                     /locus_tag="ACG8OL_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818043.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10436..10657
                     /locus_tag="ACG8OL_002068"
     CDS             10436..10657
                     /locus_tag="ACG8OL_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS4818044.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10822..11559)
                     /locus_tag="ACG8OL_002069"
     CDS             complement(10822..11559)
                     /locus_tag="ACG8OL_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS4818045.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11712..13052)
                     /locus_tag="ACG8OL_002070"
     CDS             complement(11712..13052)
                     /locus_tag="ACG8OL_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS4818046.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13289..14812)
                     /locus_tag="ACG8OL_002071"
     CDS             complement(13289..14812)
                     /locus_tag="ACG8OL_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4818047.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         4244 a         3216 c         2495 g         5020 t
ORIGIN      
        1 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     6541 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
     6601 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
     6661 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
     6721 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
     6781 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
     6841 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
     6901 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
     6961 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
     7021 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
     7081 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
     7141 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
     7201 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
     7261 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
     7321 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
     7381 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
     7441 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
     7501 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
     7561 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
     7621 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
     7681 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
     7741 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
     7801 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
     7861 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
     7921 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
     7981 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg
     8041 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
     8101 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
     8161 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
     8221 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
     8281 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
     8341 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
     8401 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
     8461 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
     8521 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
     8581 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
     8641 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
     8701 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
     8761 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
     8821 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
     8881 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
     8941 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
     9001 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
     9061 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
     9121 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
     9181 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
     9241 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
     9301 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
     9361 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
     9421 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
     9481 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
     9541 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
     9601 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
     9661 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
     9721 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
     9781 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
     9841 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
     9901 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
     9961 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    10021 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    10081 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    10141 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    10201 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    10261 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    10321 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    10381 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    10441 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    10501 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    10561 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    10621 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    10681 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    10741 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    10801 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    10861 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    10921 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    10981 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    11041 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    11101 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    11161 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    11221 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    11281 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    11341 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    11401 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    11461 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    11521 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    11581 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    11641 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    11701 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    11761 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    11821 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    11881 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    11941 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    12001 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    12061 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    12121 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    12181 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    12241 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    12301 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    12361 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    12421 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    12481 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    12541 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    12601 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    12661 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    12721 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    12781 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    12841 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    12901 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    12961 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    13021 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    13081 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    13141 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    13201 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    13261 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    13321 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    13381 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    13441 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    13501 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    13561 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    13621 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    13681 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    13741 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    13801 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    13861 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    13921 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    13981 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    14041 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    14101 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    14161 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    14221 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    14281 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    14341 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    14401 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    14461 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    14521 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    14581 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    14641 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    14701 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    14761 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    14821 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    14881 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    14941 cctattccct cctctttttt caggttttta aagct
//
LOCUS       DAZUJO010000064        14327 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000064 DAZUJO010000000
VERSION     DAZUJO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.065"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..537
                     /locus_tag="ACG8OL_002072"
     CDS             <1..537
                     /locus_tag="ACG8OL_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818048.1"
                     /translation="YTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENK
                     LEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVL
                     PVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIIT
                     LIVLLFYCIKLKIRTSHE"
     gene            complement(804..1973)
                     /locus_tag="ACG8OL_002073"
     CDS             complement(804..1973)
                     /locus_tag="ACG8OL_002073"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4818049.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            2177..3352
                     /gene="mltG"
                     /locus_tag="ACG8OL_002074"
     CDS             2177..3352
                     /gene="mltG"
                     /locus_tag="ACG8OL_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS4818050.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3488..3967
                     /gene="greA"
                     /locus_tag="ACG8OL_002075"
     CDS             3488..3967
                     /gene="greA"
                     /locus_tag="ACG8OL_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS4818051.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            4136..4867
                     /gene="liaF"
                     /locus_tag="ACG8OL_002076"
     CDS             4136..4867
                     /gene="liaF"
                     /locus_tag="ACG8OL_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS4818052.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4864..5931
                     /gene="liaS"
                     /locus_tag="ACG8OL_002077"
     CDS             4864..5931
                     /gene="liaS"
                     /locus_tag="ACG8OL_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS4818053.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            5938..6570
                     /locus_tag="ACG8OL_002078"
     CDS             5938..6570
                     /locus_tag="ACG8OL_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS4818054.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6710..7375
                     /locus_tag="ACG8OL_002079"
     CDS             6710..7375
                     /locus_tag="ACG8OL_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS4818055.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7403..7696
                     /locus_tag="ACG8OL_002080"
     CDS             7403..7696
                     /locus_tag="ACG8OL_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS4818056.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7764..8711)
                     /locus_tag="ACG8OL_002081"
     CDS             complement(7764..8711)
                     /locus_tag="ACG8OL_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS4818057.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(8889..9692)
                     /locus_tag="ACG8OL_002082"
     CDS             complement(8889..9692)
                     /locus_tag="ACG8OL_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS4818058.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9838..10752
                     /locus_tag="ACG8OL_002083"
     CDS             9838..10752
                     /locus_tag="ACG8OL_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS4818059.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10800..11270
                     /locus_tag="ACG8OL_002084"
     CDS             10800..11270
                     /locus_tag="ACG8OL_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS4818060.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            11355..11963
                     /locus_tag="ACG8OL_002085"
     CDS             11355..11963
                     /locus_tag="ACG8OL_002085"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS4818061.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            12097..12297
                     /locus_tag="ACG8OL_002086"
     CDS             12097..12297
                     /locus_tag="ACG8OL_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4818062.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            13051..14277
                     /locus_tag="ACG8OL_002087"
     CDS             13051..14277
                     /locus_tag="ACG8OL_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4818063.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         4898 a         2171 c         2861 g         4397 t
ORIGIN      
        1 tatacaagga gaagaaattt tatactatta ttaatattag ctatttcaat tttaaattat
       61 tcatcagtag ttaacatggt gaaagaaatg aattttcaag gaacttattc taatgaacaa
      121 atggaaaata aacttgaaga atttgagaat tttgattatg tacctatagg ctataaaaaa
      181 gatcaattgc ttttgaaaaa ccatcaagtt acagaagatg gtcaagttat ttatagtagc
      241 ttttctccaa ataaagatga aattacattt acgtttaaat cttcaaaagg caaaacaata
      301 gttttacctg tattccggta taaaggatta acggttactg ttaattcaaa aaatgctaca
      361 gtactaaaac aaggaggtcc ttttgttaca gtaaaaagtg aaagtggaat aaataaagta
      421 acaatcaggt attcatacac atttttttct aaaatctcat tatttatttc tataatcacg
      481 ctaattgttc ttctttttta ttgcataaaa ttaaaaataa gaacaagcca tgaatgattt
      541 gctagcacat attttagcaa tctaaagata ctattttaga tttaataggc cctttgtcaa
      601 ataatgttgg taaagagaaa tcttagaata atttggacag aacagaaaga agaacacctc
      661 tttctgttct gtttatttgg aattaaagtc tgtagcaaaa attgattttt agatttgtcc
      721 cacactcttt cctacgttta gtcgagtact ttttttaagc aaatgctatt caaagaagta
      781 gtatttgcta tcttttcatc atatcagcta tttgccaact tagtagttcc ttccatcagg
      841 tcatagattt ggtttcttag atcagcagta ggcagtttga tagttttgtc agcttttata
      901 ttgctttcca tgatgatagc atcctgtgaa tctttgctaa atatagcaga tgtatcaaaa
      961 gagtcgatat ttccttgcga caggatataa ttatctccgc tatatacccc accactatat
     1021 ggatgtccat tcgcattttc acgccataat gagaagagta agtctttgga aactagtttt
     1081 ccagataaaa cttgtgtaaa cagcgtattt aaatcataga cggacatact gatattacca
     1141 gtccctaatt cgttatggat ctctgtttca gattctgtca gtggtttaaa gtaatcatcc
     1201 tctttagaca tttgatagga tagtgcatga ttggatgaat ttttgacttg gtcataaaat
     1261 tttgtatgct gtaatcctaa aggttcgatg atatcatcgg tcaataattt ttcatacggt
     1321 tgatttgtta atttgatcaa aattccagtc aatatggtaa agttcaatga atcataatga
     1381 gaggtctgat aatctgtaaa ttttaagtgc tgaatcgcaa attgaacgac ttcttcatca
     1441 gtcatcggta tggttggaga agcagttcgg tgcaagcctg agcgcatata cagcaagtca
     1501 cggatagtaa tttgctggct gccagcaatt tgtggataga attgagccag cttagtatct
     1561 aaagaaaggt gcccacgctc gacttgtttt aaaatcaaaa gagtagtcaa tgctttttga
     1621 accgaaccaa tctgataata aagagaagga tcatttggtt gttgcgtgag tttattcgca
     1681 tagccatagc ctttttccat gatgatgcga ttatttttct ttacgtaaat tgagccaata
     1741 taatcatttt ttcttaatag ttgatcgatt ttttcaacga gctgttgttc attggtagtc
     1801 agtatcaact ttttttgagc tgattgagca tgggcaacta tctgagagtg ttcattattg
     1861 taaaattgcc atccaagaat agttcctgta gagaacgcac taaggctaaa caacacgaca
     1921 acgatcatta aaaatgaata ttttcttttt ttagcatgtt ttcctttttt cataatagtt
     1981 tatttagtct cctttctttt ttacagaaag aataaagcta aaatagcttg tatcttgata
     2041 acatttattt taatcttata gtaatggatt tactacttac atttttgata gaatatttaa
     2101 ataaaatcat tcacgccaat tcaaatatca tgtataatga ataaagttac gaacttgata
     2161 gtaaggagga gttgttttgt caggaaacaa acaacaccca agtaaagata aaaaagaagc
     2221 cagtaaggca gctacagcag atataatcga caagcggaga aatctgcgta aaaaagaaga
     2281 taagattgtg aggaagatca ttttagtgat tgctcttaca ttattgatta ttggcggatt
     2341 tcttggattt accgtgtatc gttatgtaga tagcgggttg aagcctttag ataaatcaga
     2401 tgaccaatta gtccaagtcg agatcccgag tggctcttct aacaagcaga ttggtgagat
     2461 tttggagaag gacaatatta ttaaaagcgg gattgttttc aattattata caaaatttaa
     2521 aaatttaact ggctttcaag ctggctatta tcagttagcg cctaatatga ctcttgacga
     2581 gattggcaaa cagttgcaag aaggcggtac ttctgagcca acaaaagttg cagatgggaa
     2641 aattgccatt ccagaagggt acgatatcga tcagattgct gaacgggtag cgaaggtaac
     2701 tggtaaagac aagaaagaat ttttagattt agtgaatgat gagactttct ttaacgagtt
     2761 gcaccaaaaa ttcccagaac tgttggaaag tgcatctaaa gctgagaaca caaaataccg
     2821 tttggaaggt tacttattcc cagccacata tgattattac acgagtactt cgcttaagga
     2881 tctagtgatt gaaatggtga acaagacgaa tacggtcatg caaaactatt attcagctat
     2941 caagcagaaa aatttgactg tacaagaagt tttaactcta gcttctctag ttgaaaaaga
     3001 aggcgtaaaa gaaaatgatc gtaaaaacat tgcacaagtc ttctttaacc gaatcaaagc
     3061 aaatatgcca cttcagtcag atatctctgt tttatacgca ttaggtgaac acaaagaatt
     3121 agtgacgtat gaagatacag ctgtagattc accatataat ttgtatacca ataccggcta
     3181 tggacctgga ccttttgaca atccaagcga agaggcaatc aaggcagtat tagaaccagc
     3241 agaaaatgac tattattatt tcgtagcaga tacgagcact ggaaatgttt atttcgctaa
     3301 aacttatgaa gaacatatgg aattagtgga aaaatatgta aataattctt agaaaaacag
     3361 gctctagaca ttgatcattt ttagaaaaac atttacattt gaagaaaagc gtggtaatct
     3421 tttttggact atattcctca aaagattacc ttttgcggta ttttatcgaa aaaaggagac
     3481 gttacacatg gtagagaaag tttatccaat gactctagaa ggtaaagaaa aattagaaaa
     3541 agaattagaa gaactaaaaa cagtcaaacg cggagaaatc attgaacgaa tcaaaattgc
     3601 ccgcggtttc ggagatcttt ccgaaaactc tgaatatgaa tcagcgaaag atgaacaagc
     3661 atttgttgaa ggaagaatca caacaattga aaatatgatt cgttttgctc aaatcatcga
     3721 taacgacggt gtagatgcag atgaggtatc aattggtaaa acagttacat tcatcgaact
     3781 accagatgga gaagaagaag agtacacgat tgtcggaagc gctgaagcag atccgttttc
     3841 tggcaaaatt tccaatgatt ctcctattgc gcaagcattg attggcaaac gtaaaaatga
     3901 tgaagtaact attgctactc caggtggcga tatgttagtt aaaatcgtca aagtagaaca
     3961 agcgtagtct gactgaaaga tctagagagt tcatcttact gatgatacac tctagatctt
     4021 tttctctttg tgtattttag atttcaacaa acgatcttac atctaaagtc ctatagccaa
     4081 cagttaaaaa aataggtatg atgaaatgag aatgtgtcag gagggagaaa gagcgatgaa
     4141 tagttcttgg cgtttttttg tggtagttga agcactactg ctgttatttg ctgtttggca
     4201 aatcgtaaac aatactggac ttttgctttt agtgatattc ggtattttca atatatatct
     4261 agctatgcgc aaacatcctc gtactaaatt tcaaaatttt caattgatat taggaagttt
     4321 ggtcattttt tttagcttag tcaacagtcc tgctttatgg atgatggctg tatttgccgt
     4381 tttatttatt ggtctgaaag gtgtagagat ttcagggata gatttaacca aaaatgcttt
     4441 ttggcgaaag aaacagatca tgatgatcca aacagagcaa ttaaaaacac ataataatga
     4501 gcggaaaaga cagcaattat tcggtaacca acgtattgga aacgacgtct atgaatggga
     4561 cgatatcaac atcgccgtca tttctggtga tactatcata gatttgggca atacgctttt
     4621 gccaaaagat gataatattg tgattgtaag aaaagggata ggcagaacac ggatcttagt
     4681 acctttgggc gtagccatcc gattagagca tgcgacatta gtaggaaatg tcttgtttga
     4741 agaagagcaa ttttctttga aaaatgaaca aatcaaaatc tacagcagtg attatgatga
     4801 aaaccctcgt agattgaaga ttattactaa taccttgatt ggagatgtcg aggtgatacg
     4861 aatatgatgg gaaaaatatc cagagcgatg ctagctgttt attcggggat tgcggctttc
     4921 cttattattc tattttcact tttcacttat ttttatgcca gcaatcaaag tcattggtgg
     4981 ggagaattac tgcgtgcacg tttattatat gttccgctta tcttccattt gttagccatt
     5041 tccttaggtg taggattaat tgtctttcta ttattatcac ttattcaaaa gacaaaatac
     5101 gggaaaatcg aagaaaagct gcgtgcactt tcttctggca attatgaatc caaattgttg
     5161 cttcttccga tcccaagtgc atcggacgat ttatacatca aagatattga taaggaaatc
     5221 actaagataa aagaaaaaat gattgaagta tctagtgaat tacaaattgt aacgagccgt
     5281 ccccaatatg tagatggaca aaccaaagaa gaaattttag aattagaaag acatcgatta
     5341 gcccgtgagc tgcatgattc agtttcgcaa caattgtttg cagcaatgat gatgatgtcg
     5401 gcattgacgg agcaagcaga aaaaagcgag acaccagaaa tgtttcgcaa gcagttgaaa
     5461 atggtagcag agatcatcaa cgcttcccag tctgagatgc gtgcgctgct tcttcatcta
     5521 cgtccagtca atttagaaga gaaaagttta aagcaaggta tcgagcagtt attgaaggaa
     5581 ttgcaaaata aaatccagat ttcgctgaaa tgggatgtag aagatgtaaa actatctagt
     5641 tccattgagg atcacttgtt ccgaatcgtt caagaattgt tatcaaatac attaagacat
     5701 gctaaagcca atgaattaga ggtatatttg cacaaaatag acaataatct tttgttacgt
     5761 attattgacg acggaacagg gtttaatatg aatgaaacga aaacgggaag ttatggattg
     5821 aacaatatca aagaacgagt agctggtatt ggtggaacag taaaaatcat tagttttaaa
     5881 ggtcaaggta caagtgtaga aatcaaggtc cctttgatga aggaggcata gataaggatg
     5941 ataaaagttt tattagtaga tgaccatgaa atggtgcgct taggcgtctc tttttattta
     6001 tctattcaag aagacattga agtaattgga gaagctgaaa atggacgaca aggctatgag
     6061 aaggcgatga cacttcgacc ggacgtcatt ttaatggact tagtcatgga agaaatggat
     6121 ggtatcgaat caacaaaagc aatcttaaaa gattggccag aagctaaaat cattatcgta
     6181 accagtttta ttgatgacga aaaagtttat cctgccattg aagcaggtgc agcagggtat
     6241 ttgttgaaga cctctaccgc acatgaaatt gctgatgcta taagggcaac gcaacgtggc
     6301 gagcgagtat tagaaccaga agtgacaaca aaaatgatgg aaaaaatgag tcggcgaaat
     6361 gagcctgtat tacatgaaga actaacgaat cgggaaaatg aaattttaat gctgatttct
     6421 gaaggaaaaa gtaatcagga aatagcggac gaattgttta ttacactgaa aacagtaaaa
     6481 acacatgttt ccaatatatt ggctaagcta gaagtggaag accgtacaca agccgctatc
     6541 tatgcattca aacatggttt ggtgaaataa aaacgatgat aaagaggata atcaaaagct
     6601 gagaaaataa ctattatttt tcttggcttt tttacaataa tgatgcttgc ttttgttttt
     6661 gtaaaggctt tgttatactg agaaaagaag atactaaagg aggaaaacgg tgaaacaaaa
     6721 ttttgcaatt gtaggtcttg gacgatttgg cggaagtatc tgccgaacac tgatcgaagc
     6781 tggtcaagaa gtactggcta ttgatagtaa tgaagatcgg gtcaatgaat atatgaatat
     6841 agccactcat gctgttgtgg caaatgcaca ggatgaaatg acacttcgat cattaggcat
     6901 aagaaatttc gatcatgtaa ttgtcgctat tggagaagat attcaagcca gtatcttagt
     6961 tactctgatg gtaaaggaaa tgggcgtacc aaatatttta gcaaaagccc aaaatgagta
     7021 tcatgccaga gtacttgaaa agatcggagc tgatcgcgtc gtccatcccg agcgagacat
     7081 gggaataagg attgcgcata atttagtgtc aaagaatatt ttagattacc tagagttgtc
     7141 agataaatat tctttagcag aaatccgagt ctccaatcct aaatttttca acaaaacatt
     7201 agctgaatta aatttcaggc aacgttttga cttaacagta gtcgccatac gaagagtaga
     7261 tcaaacggtt atcgcttctc ctgcagcaga tgaatatgtt cgtgaaaatg ataatctact
     7321 ggttattgga gagacaggcg atgtggatat tctagacgat aagatgaatc aatagaaaga
     7381 aaagagaaag taggttgttg ggatgaaatt aacggtaacg ccaaaagcac aaaagtggtt
     7441 tgactcagaa attgatctgc cagaaactta cggaatacgt ttttttggaa aagtctacgg
     7501 caaaacagag gttcatgatg gtttttcgat tggaatgtca gttgaacagc cagaacatcc
     7561 tgtaaagaaa gtggaaattg atgggacact tttttttatc gaggaagcag atgaatggtt
     7621 ctttaaagga tatgatttac ttgttgatta tgatcccaac ctggaggagc caacctatca
     7681 ctttaaaaaa caataaaaaa agagagggtg acacaatttt tgtcaccttc tcttttttct
     7741 tttgaaaata ttgttttcag ctattaatat cctgcagaca attttacttg attggtttga
     7801 agctcatggg tggcaaagta gcttttcaga ttggttaaaa aaatagccat taatttcttt
     7861 tgaaaatcag gtgtcaaacc tgcaatgtgg gaagtaatca gtacattgtc cattttccaa
     7921 agaggatgat cttgtggaag aggctcttcc tcaaaaacgt ctaatgctgc aaaggaaagt
     7981 tgctggttat ttaaagcctg caccagatct tgtgtcttaa cagaagcacc acggccaaca
     8041 ttgatgaata cagattttgg atcaaagtgt tcaaacatat gctgatcaaa gagcccagtt
     8101 gtttcctccg ttagaggtaa gatattgact acgaaatcta cttcaggaag aacttccttt
     8161 agttgattta aagaaaaagt cttttccatt ccttctgctg gatgaccgga tgtattgatt
     8221 ccatagcagt tcatgtctaa tgctttgcat ttttttgcaa tcatttttcc aatatggcca
     8281 gttccaatga ttagtatttt tttcccttgg atagattggt aatggaccga tgaacctaac
     8341 catttcatgt cactctgtgc cttttgtgct tgaaaaagac cacgagaata cccaaggatg
     8401 attcctaaga catgctccgt aatcgattca ctgtggatgc cactaccatt tgaaagtaaa
     8461 atatgtttct ttgaaaaagt ctcaaggggt agctgatcaa caccagctga gatagattgg
     8521 acccatttca aggtagaaga ggctaatagt ttttcttggt agtctgagtt ccagcccaaa
     8581 ctgatctcta cattttgtag atcctctggt tgtaattctg ttttaaagat ataaccaggc
     8641 gctatagtat ggatttcttc gataaattca tcacgaaatg gacgatctgc ataaatgatt
     8701 ctttctgaca tagttaattt cccgcctttc tttactctaa gtgtagcgca tacaagaaga
     8761 actttgaagc gccttgcttg ttttttgaga aagaaatatt tttcaaataa aaaaggggct
     8821 gtgacaaaaa aatgtcacaa cctctaaaag ggtctattga agtagaaata acgagttgtc
     8881 tttatttctt aattgatcga tatttttgcc catcctttga cagcttgagc aaaaataaag
     8941 attcctgcaa aagcaatgaa ggcgctagtg tcaaaagaag gaagaaagac catcaagatt
     9001 gttgcaatta tagtaggcaa tgaaattgaa tagatcaatg tttttaaaca atcaagaaaa
     9061 gtagcttttg ttagtcgaaa gcgtgcatag agaaaagcag caatgttggt aaatagcaaa
     9121 gtgatcagca gattcagaaa actaggataa atagaaaata taaatgtcag gacttttatc
     9181 caaagtggta tagatgcttc acttaaaaaa gaacggagct gcttacctgt taagtttttt
     9241 aaagattcat tggtgtaggg aagttctaat tggttattgt tcaacaaagc agaagtcgtg
     9301 cctgtattag gaagagctat gataagtttg ttttttaaca tcccgacact aacaaaattc
     9361 cccatcaaat cagtagagat gtcctgttct gttcgttttc cctctggatc aaatgtgaag
     9421 ataatggagt ttgtttggta aatgaatcct tctttttctt cggtttgaag ctgcccgttt
     9481 tggattgtaa aatcaggaat gcgagtagca atcttttgcc catccaattg aatgttgttt
     9541 aagacttgaa aaacttgaaa agagattggc aaagccataa ttatactcaa aactaaaaga
     9601 tagacaatgg ctttccaaaa tgggacattt ctcgcctcct ttaggtcttt gaatcgtata
     9661 aaggaactga taattaattg ttttgttgtc ataagtactc ctcatctagt agtgtcatca
     9721 cttgcaattc tagcgaagta tcaagtgaaa tacaagtgct ttaaggaaac agctgaatga
     9781 ttggtgaaat atcaaaaaaa tgttattctt gtctaaagac tatccaatat aaataaaatg
     9841 gaggaaggaa tagtgcctac acagccacag agatcatcaa aaaaactatt tggagtagtg
     9901 gttttgaatg cggtgatcac tttatcagaa tttattggag ggattatgtc agggagtttg
     9961 tctcttgttt cagatgcttt ccataacctt agtgatacat tggcaattat tttttcttac
    10021 ggggcacaaa aaatggcccg aaaagaagca aataaaaaac gtacgtatgg ctaccagcgt
    10081 ttagaaatat tgagtgcttt catcaactca ttcatattga tcatattatc cttgttttta
    10141 accgttgaag cattcaagcg gttcacttca ccagaaaaga taaatagtca tttgatgcta
    10201 acagtagcag ttattggatt gctcgcgaat ttgttctcag tattattatt acgacaagaa
    10261 gcagatgaaa gtttgaatat aaaatctagt tatttgcatt tgttaagtga tacactgtct
    10321 tcggtaagtg tgattatcgg agcaattttg atccgttttt tcgatatcta ctggatcgat
    10381 ccagttatta cactgatcat ttcgatatat atattgatag aagcgataat ggtgatcaaa
    10441 aaagctgccg ctattttgat acaaagtgca cctgctatcg actatgaaaa aatggaacaa
    10501 gaaattgaag caatcgatgg ggtaaaggat gttcatcatg tccatatttg gcagtatagc
    10561 gaaaagataa ttattttcga cggacatatt gattttgaag atcagctact aagcgagatt
    10621 gaaaagatat atcctcgtat aatttcttta ttaaaattaa aatatggtat tacacatgtg
    10681 acgatccaag cagagacaaa ggcaaaggat caaaaaaaat taattttttt aaataaagat
    10741 gaaagttatt gaaatataga gtatgataaa taagaatttt taataaggta ggttggctca
    10801 tgaagcagta tcgacagtta aaaggatatt tagaagcaaa aggatattgg gagatactag
    10861 tctatctctt ttttggtggt ttggctacga ttgtaaattt tgttagtttt gctctcgcac
    10921 gccaatattt tgatttaagt atggccttat ccaattctat ttcgtggttt tgttctgtat
    10981 tgtttgcttt tgtaacgaac aaactttggg tattccattc aaaatcaccg aattttacac
    11041 atgcacttat tgaatgcggt aagttcttct tctaccgtat cctttcttac ggattggaca
    11101 tgggggcaat ggtattgttg atcaatgtta tgaacagcaa tgagtatgtg gcgaaaatca
    11161 tcactcaaat tattgttatc cttgcgaact atattttcag caagttgttt atttttaaag
    11221 aaactgaggt ttttgaagaa gaagtaggtc aaagtgaaaa agacgactag gcataacaat
    11281 tgaagaattt ccttctcttt gttactcttt gtaatgtaga cattattaag aaaacattga
    11341 ggaggaattt ttttatgact tacacattac cagatctacc ttatgcatat gacgcattgg
    11401 aaccttatat tgacgaagaa acgatgcatc tgcatcatga taagcatcac aatacttatg
    11461 tgacgaattt aaattcagca attgagaaat acccagaatt aggcgaaaaa acaatagaag
    11521 aattattatc tgatatggac gctattccaa cagatatcaa gacagctgta cgtaacaatg
    11581 gtggcggaca tgctaaccat tcatttttct gggaaatcat ggcaccaaat gctggtggcg
    11641 aacctacagg agaaataaaa gaagcgatta atgaagcttt tggtgatttt tcttctttta
    11701 aagaagaatt caaaaaagca gccgctggac gatttggttc tggatgggct tggcttgtaa
    11761 tggaaaatgg aaaattagct attacctcta ctgcaaatca agattctcca ttgatggaag
    11821 gaaagacacc aattctaggt ttggacgttt gggaacatgc ttattatttg aaatataaaa
    11881 atgtccgacc agattatatt gcagcattct ggaatgttat taattgggat gaagtaaaca
    11941 aacgtttcga agcagcaaaa taaccatttc ctccgtcata tcagtcttaa aaataatttg
    12001 gaaaataaaa gagcgaaagc ttgctagcgt tttctaaatt gggtataatg ttcttgcaag
    12061 gttacttgca agaacttttt tggaggatat acatttatgg aacacggaac ggttaaatgg
    12121 ttcaacaatg aaaaaggatt tgggtttatt acagtagacg gcggagatga cgtatttgtt
    12181 catttctcag caattcaagg agacggcttc aagagcctag aagaaggtca agaagtagaa
    12241 ttcacgattg ttgaaggtgc acgcggacct caagctgctg aagtgactaa attataataa
    12301 tttgttgaat aaagacacga cttcttagag agtcgtgtct ttttgtctat ttatgaagtt
    12361 tttagatttt ttggctaagt aaatgcattt gttcacttga catgtcctaa ctaattatca
    12421 agcaaggaac attttcttcg ttagtaatgg attttaaaga aatttcagtt tttggatgtt
    12481 gaataaaaga ccatttgtga aaccactagt atacatactc attaccatgt caattctatt
    12541 actttaaaag aaaatcgttt tactttgaag aggagcatat ctatccatta aaagatgatg
    12601 gtaatgcaat cactagttcg agtcatttta agagccatgc agtgagctgg aaataaaacg
    12661 aatagaagat gggtttaaca aaaggaccag tcataggaag aaattgtcgt gaaatcgaat
    12721 gataatctga acggtaggca aagactgtta aaaagatggg aaaggtatgt gcgatcattt
    12781 tactgcacga ttatttggaa aataccttgt tgttcgcttt ttctggaaaa aatacataaa
    12841 aaaagataaa acgattgcga actgcgtgta ttcggtcttt cgatcttttt cacggtatgg
    12901 tatgatagtt aagaattttg agaaatttta aaaaataata atcgttacac aaaaatgttt
    12961 tttaaagttt tcttatgatt aaaaagtgaa tttgctgatt gaacagttat atgagacaat
    13021 attttagaaa atgtgaaaca gaggtgtatt atgaagcgat ggcaaaaagt tctattaacc
    13081 ttgttagggg tattcgtttt gctgattgcc ggattatcag catacggaat cagatttatg
    13141 ggagaagcga gccaaacagt aaacaaaatt tctaaaaact ctaatcgaat atcctccaaa
    13201 cgttctgaga gagtaagcat tgatgacaaa gaaccttttt cggttttgct attaggttta
    13261 gataccggtg gattaggaag aacggaacaa ggacgttctg atacgatgat ggtagtaaca
    13321 gttaatccga aacaaaaaaa atcaacgatc atcagtttgg accgagatat ttatacgaat
    13381 atcgtaggtt atggaacggt agataaatta aatcatgcct acgcatttgg tggtgtcgaa
    13441 atggccatgg attcaattga gcaactgctc gatattccta ttgatcatta tgtaacgatc
    13501 aatttggatg gcatggaaga tttgatcaat gcggtcggtg gcatcaaagt gaataataaa
    13561 attgacttta ctttggatgg tgtccatgta ccagtaggtg aacaaacatt gaatggagaa
    13621 aaaggtttag cctattctcg aatgcgacac gaagatccag aaggcgatat cggtcgacaa
    13681 cgtcgtcagc gggaagtagt gacgaaaatc gtcaataaag ttttaagttt agatggcgta
    13741 agcaattata gaaaaatttt aaaagcagtt gagaacaatg tgacgacgga cttagattgg
    13801 gatgacatgc ttgatgtagc gacgaactac accccggcgt ttgaaacaat caaacaagac
    13861 cagttgcaag gcgaaggaca aatgatcaat gatatctact atcaaatttt aggaaaaaat
    13921 gatcttcttt cgatccaaaa tgaactgaag aaacaattaa atataaaaac aagcgataca
    13981 ttacctaatt tgaaaaatga gaatgcttca atgatgttct ttgatgattc ggaagatggc
    14041 gattcgacaa acggtgatgg atcgaatagt gaagggtaca ccgattattc ttcctattca
    14101 gaaaacacat atgatcagta ccaagatccg aatacctatt atcagcaaac agaagataca
    14161 agtcagtact atacaccaag tcaagatact caaagttatg atccttacgc tggacaggaa
    14221 caaaatggtt atggttatga ccagaataat gagtatggaa caacaagtgg gtactagaaa
    14281 aaaatagttt ggttaataaa gagtgccacc taaattagac aaaaaat
//
LOCUS       DAZUJO010000065        14189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000065 DAZUJO010000000
VERSION     DAZUJO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.066"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..483
                     /locus_tag="ACG8OL_002088"
     CDS             <1..483
                     /locus_tag="ACG8OL_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFS4818064.1"
                     /translation="FGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIET
                     ALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNG
                     ILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI
                     "
     gene            805..975
                     /locus_tag="ACG8OL_002089"
     CDS             805..975
                     /locus_tag="ACG8OL_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818065.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            989..1591
                     /locus_tag="ACG8OL_002090"
     CDS             989..1591
                     /locus_tag="ACG8OL_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818066.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1607..2284
                     /locus_tag="ACG8OL_002091"
                     /pseudo
     CDS             1607..2284
                     /locus_tag="ACG8OL_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2595..3092
                     /locus_tag="ACG8OL_002092"
     CDS             2595..3092
                     /locus_tag="ACG8OL_002092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS4818067.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            3093..3509
                     /locus_tag="ACG8OL_002093"
     CDS             3093..3509
                     /locus_tag="ACG8OL_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS4818068.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3529..5073
                     /locus_tag="ACG8OL_002094"
     CDS             3529..5073
                     /locus_tag="ACG8OL_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818069.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            5216..5440
                     /locus_tag="ACG8OL_002095"
     CDS             5216..5440
                     /locus_tag="ACG8OL_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818070.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5455..6324
                     /locus_tag="ACG8OL_002096"
     CDS             5455..6324
                     /locus_tag="ACG8OL_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4818071.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            6305..7039
                     /locus_tag="ACG8OL_002097"
     CDS             6305..7039
                     /locus_tag="ACG8OL_002097"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4818072.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            7072..7980
                     /locus_tag="ACG8OL_002098"
     CDS             7072..7980
                     /locus_tag="ACG8OL_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4818073.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7977..8519
                     /gene="sat4"
                     /locus_tag="ACG8OL_002099"
     CDS             7977..8519
                     /gene="sat4"
                     /locus_tag="ACG8OL_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4818074.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8612..9406
                     /locus_tag="ACG8OL_002100"
                     /note="aph(3')-IIIa"
     CDS             8612..9406
                     /locus_tag="ACG8OL_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4818075.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9682..9813
                     /locus_tag="ACG8OL_002101"
                     /pseudo
     CDS             9682..9813
                     /locus_tag="ACG8OL_002101"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9889..10382
                     /locus_tag="ACG8OL_002102"
                     /pseudo
     CDS             9889..10382
                     /locus_tag="ACG8OL_002102"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10552..11448
                     /locus_tag="ACG8OL_002103"
     CDS             10552..11448
                     /locus_tag="ACG8OL_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4818076.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11547..11756
                     /locus_tag="ACG8OL_002104"
     CDS             11547..11756
                     /locus_tag="ACG8OL_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4818077.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11773..12045
                     /locus_tag="ACG8OL_002105"
     CDS             11773..12045
                     /locus_tag="ACG8OL_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4818078.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            12047..12910
                     /locus_tag="ACG8OL_002106"
     CDS             12047..12910
                     /locus_tag="ACG8OL_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4818079.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACG8OL_002107"
     CDS             complement(13173..13910)
                     /gene="erm(B)"
                     /locus_tag="ACG8OL_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4818080.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14035..14118)
                     /locus_tag="ACG8OL_002108"
     CDS             complement(14035..14118)
                     /locus_tag="ACG8OL_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFS4818081.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         5018 a         2222 c         2928 g         4021 t
ORIGIN      
        1 tttggtcacc ttgaaggtga tacgatcgtt ggcattcatc ataaaagtgc cgtcattact
       61 ttagttgaaa gattatctaa agtcattatc acgattaaac ccaacggccg taaggcatta
      121 gatattgaaa ctgcccttaa tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct
      181 attacgtttg actgtggaaa agaattttct aactggaaag ccattagtaa ccaacatgat
      241 attgatatat attttgcgga ccctggaaca ccttctcaac gcccattaaa cgagaattct
      301 aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg attttagaaa agtgaatcag
      361 acatttattt ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac
      421 agaacaccaa tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt
      481 tgacaaatca tactatgata cgtgtttgtt tttctgtgct gtttagtgaa tgattagcag
      541 aaatatagag agtaagattt taattaatta ttagggggag aaggagagag tagcccgaaa
      601 acttttagtt ggcttggact gaacgaagtg agggaaaggc tactaaaacg tcgaggggca
      661 gtgagagcga agcgaacact tgatctttta agttgctatc tttttaaggt caatagagta
      721 tacttatttg tcctaatatg gttttagcag tataattgac ttggtgaata ggtcatttaa
      781 gttgggcata ataggaggag taaaatgaaa aaatttattt atcgagtttt agaaaatgac
      841 gaagtggtgg ctatttttaa tgagcaacaa tatgcgcaag attttatcgc ttacgaaaag
      901 acaatttctg ataagcaatt tgaaattgaa aaagtagata tagctgattg gttattgcaa
      961 ccgagagaat tttagaggtt ggttgaaaat ggctaaaatt ggttatgcac gtgtcagtag
     1021 caaagaacag aacttagata gacaactgaa agcgttagag ggcgtttcta aggtcttttc
     1081 agacaaatta agcggtcaat cggtcgaacg cccacaatta caagctatgc ttaactatat
     1141 tcgtgaaggg gatatagttg ttgttactga attagatcga ttaggacgaa ataataaaga
     1201 attaacagaa ttgatgaatc aaattcaaat taagggggca accctggaag tcttaaattt
     1261 accctcaatg aatggtattg aagatgaaaa tttaagacgg ctgattaata atttagtgat
     1321 tgaattgtat aagtaccaag cggaatctga acgcaaacga attaaagaac gccaagccca
     1381 aggaattgaa attgctaaga aaaaaggaaa attcaaaggg cgacaaccta aattcaaaga
     1441 aaatgatcca cgtttacaac acgctttcga tttatttttg aatggttgtt ccgataaaga
     1501 agttgaagaa caaactggaa ttaatcgccg aacgtttaga aggtatcgat caagatacaa
     1561 cgtgacagta gatcaaagaa aaaaacaatg aaaagaggga tagttaatga gtacggttat
     1621 tttagctgaa aaaccaagcc aggcattagc ctacgcaagt gctttaaaac aaagcaccaa
     1681 aaaagacggt tattttgaga tcaaagaccc actatttaca gatgaaacgt ttatcacctt
     1741 tggttttggg catttagtcg aattagcaga accaggtcat tataacgaaa agtggcagaa
     1801 ctggaaactt gaatctttgc cgatttttcc tgatcgatac gattttgaag ttgcaaaaga
     1861 taagggaaag cagtttaaaa ttgttgctga acttctcaaa aaggcaaata caattattgt
     1921 cgcaacagat agcgacagag aaggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa
     1981 tgccttttct aaagataaaa cgtataaaag attatggatc aatagcttag aaaaagatgt
     2041 gatccgaagc ggttttcaaa atttgcaacc aggaatgaat tactatccct tttatcaaga
     2101 agcgcaaaca cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttatatac
     2161 cttgaattta caacaaaaag gcgtacaagg tacattttca ttaggacgtg tacaaacacc
     2221 gactttatat ctcatttatc aacgccagga agcaatagaa aactttaaaa aagaaccttt
     2281 tttcctgaat aattcatagt tttactaata gtctttccta acattgattt aatagtcttt
     2341 aaagtattct gcttcatcac ccattaggtg atgaaaaaag aacccctttc tgatttagcg
     2401 gacaaatact ttgacctaat ctcagaagat ataaaagaaa aaataataaa atatccgatt
     2461 tatactttgt ataaagaact aaagtagtta gcaaatcatt tacagcaaat cactatgttc
     2521 cacataccat tttacatact taccttatcc acgacacatg ttgagatagt atcgaagata
     2581 aagaaagttg ctccatgttg tttttgcgag atggggaaat caaagccaca ttgacaacca
     2641 tgatgaataa gcttgctttc agtcataagc taatcctaga accacttttc aagtcagtta
     2701 gccaaatcga tgaagaaagc gaccgtgaaa gaatggacgc tattgataag ctaatggagc
     2761 aactattgga agaacgcaat acccttattg cactgatgtc caaaggtttc ctagaacctg
     2821 ctctttttaa tcaggaacga aatgttttag atagtgagat taaaaatctt actaccgaaa
     2881 aaacaaacct ggtaacgaac tctgcgagtg gggttttgcg agcaaacgag ataaaggacc
     2941 tcattaatta tgtgtcagca gataatttta atggtgacta cacggaagaa ctatttgaag
     3001 aatttgtagt gaacatcatt gtaaattcca gggatgagct gacattcaat ttgaaatgcg
     3061 gtctttccct gaaagaaaag gtggtgagat aaatggcata tattccatat ggatacaaaa
     3121 ttcaagatgg agtggttact gtcgatgaaa aggcagcagg tcaagtaaag gtattctttg
     3181 agaaatacat atcaggacta tcccttacag tggctggcga acaggcaggt attgaaaaga
     3241 cacattctgt gatggggcgc attttgaaaa acgtcaacta ccttggaaat gatacgtatc
     3301 cagcaattat tgataaagag atatttgata aagctgaaga agttagagat aaacgtgcaa
     3361 aggatttagg acgagtggta gagcttgccg ctttcacctc tccccctccc aaagaacgat
     3421 tcaaaatgaa aaaggcagat aataagatgc cagtggatcc ttttgaacga gcagaatact
     3481 tatacagtct gatagaaagc gaggaataaa gtgacagaga aaaatataat ggttattcct
     3541 gctcgtaaaa gagtaggaag tacagccgca aaagaaaaga taaagaaact tcgtgttgct
     3601 gcctattgcc gtgtttctac agaaacagaa gaacaaaatt ctagctatga ggttcaggtc
     3661 tcacactata cagagtttat aaagaaaaat aatgaatggg agtttgctgg catctttgca
     3721 gatgatggta tctctggtac gaacactaaa aagcgtgacg aatttaatcg catgattgca
     3781 gagtgtatgg acggtaacat cgacatggtt attacaaaat ccatcagccg atttgcgcgt
     3841 aacaccctag actgccttca atacattaga cagctcaagg ataagaacat atcagtttat
     3901 tttgagaaag agaacatcaa caccacggat gccaaaggtg aggttttgct gactattatg
     3961 gcatctttgg cacagcagga aagccagagc ctttcacaaa acgttaagct tgggctacag
     4021 taccgatacc aacaaggaaa ggtgcaggtc aaccataagc gatttatggg ctacacgaaa
     4081 gatgaagatg gaaatttaac tattgtcccc gaagaagctg agattatcaa acgcatctac
     4141 cgagaatacc ttgagggtca gagtctagtg ggcattggtc gagcccttga aaaggatggt
     4201 attttaacag cagcgggaaa accaagatgg cgaccagaat cagttaagaa gatacttcaa
     4261 aacgaaaaat acatcggaga tgcccttctg caaaagactg tcacagtaga ttttctgacc
     4321 aagaaacgag tgaaaaatga agggcatgtt ccccaatatt atgttgaaaa tagccatgaa
     4381 gcgattattc ctaaagagtt atttttgcag gcgcaagaag aaattcatcg gagaagcaat
     4441 atctacacag gagaaggcaa gaacaaaaga atttacagta gcaagtacgc tttaagtgcc
     4501 atcaccttct gtggagattg tggcgatatt tatagacggg tctattggaa tatccacgga
     4561 agaaaagaat ttgtctggcg atgcgtgact agaatcgagc aaggtcctga agtttgtaag
     4621 aaccgaaccg taaaagaaga tgaactttat ggtgcagtaa tgactgcgat taataagcta
     4681 cttgcaggtg ggaacaatat gataaagaca ctggaagaga atattcatgc tgtgattggc
     4741 gaaaccacag aataccaaat ttcagagatt aacaactcac tagaggaaaa acaaaaagaa
     4801 ctaatcaagc tggcgaacaa gggtcaagac tatgaccact tagcagatga gattgatgag
     4861 ctgagagaca agcgacagat ccttttagta gaagatgcct ccctcagtgg cgagaacgag
     4921 cgaatcaatg agctgattga atttatccgc aagaacaaat tccgcacctt agaatatgat
     4981 gataagcttg taaggaagat aatccagagc gttacagtct atgaaggcca cttcgtcata
     5041 gccttcaaat ctggcatcga aatggaaata tgaaaaccag aagtaaatag cccatgactc
     5101 tacagtagag ttgtgggttt tcttttgctt gctgattata ataatatttt aataactatt
     5161 gtgtttatca accaaatggt ttataatatt cttagcgtaa acttggaggt gctgtatgac
     5221 tacggcagaa atgattaaag aactgtgtga gcaaatgaat ataagtgttt ccgaacttgc
     5281 tagacgtatt ggccagactc cacagaattt caataaaaaa ttacaacgag aaacagtaac
     5341 cttggatgag ttgaaggcca ttgctgatgt gctaggtgtc aagtttgtgc aggcatttat
     5401 tttaccagat ggagaagaaa taaaaatatc taacgaataa aggaggatgt ttatatggta
     5461 ggtaatgtta ttcaaatagt aacagagaaa ttatcatctt tgccttttat agaaggcatc
     5521 gtattagggg gctcacgtgc aagaggcacc catacagaaa attctgatat agatatcgga
     5581 atctattaca attcagactc atttgacctg acagcgatta atcaaattgc tacagagttg
     5641 gatgatgaga atagaaacaa ccttgttgta cctcccggag cgtggggtga ttgggttaat
     5701 ggcggtggat ggttagttat aaacgggtgt catgttgact tgatattacg tgatataaaa
     5761 cgggtggaac aaataatcaa agatacggag caaggaattg ttactgccaa ttatcagacc
     5821 gggcatcccc atggttatat aagtgctatg tatcgtggag aattagcgat tagtaaaata
     5881 ctatatgcta agaacgaaag cttatgcgaa ttaaaaaagc aggcagaaat ttaccctact
     5941 gctctaaaga aaagcttgat gaactttttt atatttgaag ctgaattctc tttaatgttt
     6001 gtaaaagcaa atgcgggggc agaggataaa tattatattg caggccatgt ttttcgtatt
     6061 atttcatgct taaatcaagt actatttgca tgtaataatg cttattgcat taacgaaaag
     6121 aaagctataa aactgcttga aacttttgaa tataaacctg aaaaatatgc cgaaagagtt
     6181 aatcatattt ttgaagtact cggtctttct ctttttgaat gctacgacat gaccgagaaa
     6241 ctttataaag aagtgaatga aattgtatcg gagataaata actttttaaa cgaggagagt
     6301 tcagatgaaa gaaaacaaat atgatgataa tatatttttt caaaaataca gtcaaatgag
     6361 tcgctcgcag aaaggactgg ctggtgcggg agaatgggag actttgaaaa agatgctacc
     6421 tgattttaag ggtaagcgtg tgcttgattt aggatgcggc tatggatggc actgtatata
     6481 tgcgatggaa aacggtgctt cctctgtagt aggtgttgat atttctcata aaatgctcga
     6541 agtagcaaaa ggaaaaaccc attttccaca gattgaatat gaatgctgtg ccatagaaga
     6601 tgtggatttc ccagaggaga gctttgatgt aatactaagt tcgcttgcgt ttcattatgt
     6661 agcagactat gagaatttaa taaaaaagat atataggatg ctgaaggctg gtggcaattt
     6721 agtttttaca gttgaacatc ctgtttttac tgctcatgga acacaagact ggtattataa
     6781 cgaaaaagga gaaatactgc atttcccggt ggacaattat tattatgagg gcaaacggac
     6841 agctatgttt ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa
     6901 tacactgctt tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa
     6961 catgatggat attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc
     7021 ggcaaaaaag aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca
     7081 gaaaaagaaa tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt
     7141 gtgacccttg aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat
     7201 gatattacat attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat
     7261 caatttggga atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa
     7321 gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta
     7381 ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt
     7441 gataaagatt gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga
     7501 aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat
     7561 gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt
     7621 gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa
     7681 ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag
     7741 aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta
     7801 tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat
     7861 ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt
     7921 aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga
     7981 ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg
     8041 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     8101 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     8161 attgccttga atatattgac aatactgata agataatata tctttactac caagacgata
     8221 aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata
     8281 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     8341 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     8401 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     8461 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     8521 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     8581 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     8641 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     8701 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     8761 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     8821 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     8881 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     8941 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     9001 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     9061 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     9121 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     9181 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     9241 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     9301 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     9361 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     9421 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     9481 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat
     9541 attagagatg taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt
     9601 gcaatgagaa gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa
     9661 gtcacagagg aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag
     9721 ctgcaatcaa aaaaaataat tcctgccaaa gaaattgcac agcgatttaa tataagctta
     9781 aggacagttt acagagatat ccggacactt gaagaagccg gaataccaat tggatctgag
     9841 gccggaaaag gatattttct tgtagagggc ttcctacttc cgccggtaat gtttacagcg
     9901 gcggaagtgg gtgcattgat tacagcaggg aaatttttaa attgccatgg agatgaatca
     9961 tttataaagg attttgattc agctatgtat aaaatcaaat ctattttgaa gcatggtgaa
    10021 aaaaactatg cacaggagtt ggagaacagt attaacgtgt acagcacatc tggacagaaa
    10081 aatacattgg cggataacgt tattgcagca attcagactg caatctgcaa taaaagggta
    10141 atatcaattc aatatcctgc atcaggaggg caagaaccgg aaagcagaat gatagagcct
    10201 atatcactgg ggttttatga acagaactgg tacttaatcg gttttgcagg ctaagaaatg
    10261 aatataggaa ttttcgtgtg gatcggatta aaagcatttg catagaagaa gagctctgtc
    10321 aaagccatga tggctctttg gaacaaatat taaaacaaat gctttcatat aaaaaactat
    10381 atgccctaca atgccgttgt aaaaattggg gaaaagggat atattgattt tatatctttc
    10441 tcaaaataaa tataaaagcc ctttaaagag ggcttttata tattaatcac aaatcactta
    10501 tcacaaatca caagtgatta atcacaaatc acttgtgatt tgtgattctt aatgatacaa
    10561 tattactata caaaaaaaga atggggcgta gttatggaga aggaaaaact aaaaatactt
    10621 gaagaattaa gacgtatttt aaacaataaa aatgaagcaa ttattatctt gaataattac
    10681 tttaaaggtg gtgttggaaa gtccaaatta tcgactatgt ttgcttactt gacagacaaa
    10741 ttgaatttaa aagttttaat gatcgataag gacttacagg caacattgac aaaagactta
    10801 gcaaaaactt ttgaggtaga attgccacgt gtcaattttt atgaaggctt gaaaaatgga
    10861 aacttggctt cttctattat tcatttgact gataatttag acttgatccc tggcacgttt
    10921 gatttgatgt tactgccaaa attaactcgc tcatggacgt ttgaaaatga aagtagattg
    10981 cttgctactc ttttagcacc tttaaaaagt gactatgatc ttattattat tgatactgta
    11041 ccaacgccaa gcgtttatac aaataatgca atcgtggcga gtgattacgt tatgatccct
    11101 ttacaagcag aagaagaaag tacaaacaac attcaaaact atatttccta tttgattgat
    11161 ttgcaagaac agtttaaccc tggactagat atgattggtt ttgttcctta tttagttgat
    11221 acggacagcg caacgataaa atcaaacctg gaagaactgt acaaagaaca taaagaggat
    11281 aatttggttt tccgaaatat tatcaagcga agtaataaag taagtacttg gtctaaaaat
    11341 ggcattacag aacacaaagg ctatgacaaa aaagttttgt ctatgtatga gaacgtattt
    11401 tttgaaatga ttgagcgaat cattcaatta gaaaatgaaa aagaatagaa tcacaaatca
    11461 caagtgatta atcacaaatc acttgtgatt tgtgataggt gatgataaaa taaatagtaa
    11521 gaagaaatag aaagaagtga gcgatcgtgg gaaatttagg cgcacagaaa gcaaaacgaa
    11581 atgatacgcc aatcagcgca aaaaaagata taatggggga taagacggtt cgtgttcgtg
    11641 ccgacttgca ccatatcata aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag
    11701 ttatggatca agccttagaa gaatatatac ggaaatattt acctgacaaa ctttaaaagg
    11761 agtgaaaatg aaatggcagt tacgtatgaa aaaacatttg aaattgaaat cattaatgag
    11821 ttatcggcaa gtgtttataa tcgggtatta aattatgttt tgaatcatga actagatact
    11881 aaaaatactc gtttactaga agtgaatctt ttaaatcaat tagaagtggc acaagaagtt
    11941 gatttatttc aacaaccatt tgaagaatta caagctattc atgagtattg gcggtcaatg
    12001 aatcaatatt caaaacaaat tttgaataaa gagaaagtgg cttaacatgg cgaatattac
    12061 tgacttcacc gaaaagcaat ttgaagatcg tttagaaaag aatgttgaac gactaactaa
    12121 aaatagacta gcggttgaat cgccaaccgc ttttttactt ggtgggcaac cagggtcagg
    12181 gaaaaccagt ttgcgatcag caatttttga agaaacacaa gggaatgtta ttgtcattga
    12241 taatgatacc tttaaacaac agcacccaaa ttttgacgaa ttagtgaaac tctatgaaaa
    12301 agacgtagta aaacacgtta ccccttattc gaatcgcatg acagaagcac ttattagccg
    12361 tttaagcgat caaggctata atttagtgat tgaaggaaca ggacgaacaa cagacgttcc
    12421 cattcaaaca gccacaatgc ttcaatctaa aggttatgaa acaaaaatat acgtcatggc
    12481 agtaccgaaa attgaatcat acttaggaac gattgaacga tatgaaacca tgtatgcaga
    12541 tgatccaatg acagctaggg caacaccaaa acaagcgcat gatattgttg tcaaaaactt
    12601 accgaccaat ttagaaaccc ttcataaaac gggcttattt agcgatataa ggctttacaa
    12661 tagagaagga gtgaaactct attcaagctt agaaacgcct tccattagtc cgaaagagac
    12721 cttagaaaga gaattaaatc gtaaagtatc agggaaagaa attcaaccga ctttggagag
    12781 aatagagcaa aaaatggttc aaaatcaaca ccaagaaacc cctgaattta aagcaattca
    12841 acaaaaaatg gaaagcttgc agctacctac accaccaata cccaaaacac ctaaacttcc
    12901 aggtatttaa accttaaaaa aggaaaagag tagttaccaa aaaacggtaa ctactctttt
    12961 tttataaaaa catttcctca tgttggggtc ccgagcgcct acgaggaatt tgtatcgata
    13021 agaaatagat ttaaaaattt cgctgttttt ttctaatttc attttgaggt gaattaattg
    13081 gtagttatta gaggtgccct atacctaata atttatctac attcccttta gtaacgtgta
    13141 actttccaaa tttacaaaag cgactcatag aattatttcc tcccgttaaa taatagataa
    13201 ctattaaaaa tagacaatac ttgctcataa gtaacggtac ttaaattgtt tactttggcg
    13261 tgtttcattg cttgatgaaa ctgattttta gtaaacagtt gacgatattc tcgattgacc
    13321 cattttgaaa caaagtacgt atatagcttc caatatttat ctggaacatc tgtggtatgg
    13381 cgggtaagtt ttattaagac actgtttact tttggtttag gatgaaagca ttccgctggc
    13441 agcttaagca attgctgaat cgagacttga gtgtgcaaga gcaaccctag tgttcggtga
    13501 atatccaagg tacgcttgta gaatccttct tcaacaatca gatagatgtc agacgcatgg
    13561 ctttcaaaaa ccactttttt aataatttgt gtgcttaaat ggtaaggaat attcccaaca
    13621 attttatacc tctgtttgtt agggaattga aactgtagaa tatcttggtg aattaaagtg
    13681 acacgagtgt tcagttttaa tttttctgac gataagttga atagatgact gtctaattca
    13741 atagacgtta cctgtttact tattttagcc agtttcgtcg ttaaatgccc tttacctgtt
    13801 ccaatttcgt aaacggtatc ggtttctttt aaattcaatt gttttattat ttggttgagt
    13861 actttttcac tcgttaaaaa gttttgagaa tattttatat ttttgttcat gtaatcactc
    13921 ctgaagtgat tacatctata aataaataca gaagttaaac gatttgtttg taattttagt
    13981 tatctgttta aaaagtcata agattagtca ctggtaggaa ttaatctaac gtatttattt
    14041 atctgcgtaa tcactgtttt tagtctgttt caaaacggta gatgttttat ctacattacg
    14101 catttggaat accaacatga cgaatccctc cttcttaatt acaaattttt agcatctaat
    14161 ttaacttcaa ttcctattat acaaaattt
//
LOCUS       DAZUJO010000066        14141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000066 DAZUJO010000000
VERSION     DAZUJO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.067"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG8OL_002109"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACG8OL_002109"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS4818082.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG8OL_002110"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACG8OL_002110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS4818083.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACG8OL_002111"
     CDS             8118..9086
                     /locus_tag="ACG8OL_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4818084.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACG8OL_002112"
     CDS             9104..10009
                     /locus_tag="ACG8OL_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818085.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACG8OL_002113"
     CDS             10006..10812
                     /locus_tag="ACG8OL_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818086.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACG8OL_002114"
     CDS             10969..11937
                     /locus_tag="ACG8OL_002114"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS4818087.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACG8OL_002115"
     CDS             complement(12254..12778)
                     /locus_tag="ACG8OL_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS4818088.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14141
                     /gene="scm"
                     /locus_tag="ACG8OL_002116"
     CDS             13065..>14141
                     /gene="scm"
                     /locus_tag="ACG8OL_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4818089.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSD"
BASE COUNT         4005 a         3052 c         2789 g         4295 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 atgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga c
//
LOCUS       DAZUJO010000067        14068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000067 DAZUJO010000000
VERSION     DAZUJO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.068"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACG8OL_002117"
     CDS             279..1127
                     /locus_tag="ACG8OL_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS4818090.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACG8OL_002118"
     CDS             complement(1298..1639)
                     /locus_tag="ACG8OL_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS4818091.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACG8OL_002119"
     CDS             1725..2432
                     /locus_tag="ACG8OL_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS4818092.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACG8OL_002120"
     CDS             2419..2742
                     /locus_tag="ACG8OL_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS4818093.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACG8OL_002121"
     CDS             complement(2831..3868)
                     /locus_tag="ACG8OL_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4818094.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACG8OL_002122"
     CDS             complement(4315..5298)
                     /locus_tag="ACG8OL_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS4818095.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="ACG8OL_002123"
     CDS             complement(5714..6484)
                     /locus_tag="ACG8OL_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818096.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="ACG8OL_002124"
     CDS             complement(6477..7412)
                     /locus_tag="ACG8OL_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818097.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="ACG8OL_002125"
     CDS             complement(8006..9655)
                     /locus_tag="ACG8OL_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS4818098.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="ACG8OL_002126"
     CDS             complement(9850..10746)
                     /locus_tag="ACG8OL_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4818099.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="ACG8OL_002127"
     CDS             complement(10739..11419)
                     /locus_tag="ACG8OL_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4818100.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="ACG8OL_002128"
     CDS             complement(11420..12202)
                     /locus_tag="ACG8OL_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS4818101.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="ACG8OL_002129"
     CDS             complement(12195..13112)
                     /locus_tag="ACG8OL_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4818102.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="ACG8OL_002130"
     CDS             complement(13115..13399)
                     /locus_tag="ACG8OL_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818103.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4162 a         3005 c         2455 g         4446 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt ttcttcgtat
    13921 cattgaataa gtttgaaaag ccttgattta taaaggtttt tccgtaaata tattttgtta
    13981 tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa aggcagtata
    14041 tttcgtattt tatattaaat ttttaggc
//
LOCUS       DAZUJO010000068        13953 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000068 DAZUJO010000000
VERSION     DAZUJO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.069"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..176)
                     /locus_tag="ACG8OL_002131"
     CDS             complement(<1..176)
                     /locus_tag="ACG8OL_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS4818104.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
     gene            complement(655..1374)
                     /locus_tag="ACG8OL_002132"
     CDS             complement(655..1374)
                     /locus_tag="ACG8OL_002132"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS4818105.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1423..3069)
                     /locus_tag="ACG8OL_002133"
     CDS             complement(1423..3069)
                     /locus_tag="ACG8OL_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS4818106.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3248..3838
                     /locus_tag="ACG8OL_002134"
     CDS             3248..3838
                     /locus_tag="ACG8OL_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4818107.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3930..5435
                     /locus_tag="ACG8OL_002135"
     CDS             3930..5435
                     /locus_tag="ACG8OL_002135"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS4818108.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5519..6871)
                     /gene="celB"
                     /locus_tag="ACG8OL_002136"
     CDS             complement(5519..6871)
                     /gene="celB"
                     /locus_tag="ACG8OL_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4818109.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6871..7389)
                     /locus_tag="ACG8OL_002137"
     CDS             complement(6871..7389)
                     /locus_tag="ACG8OL_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818110.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7405..7731)
                     /locus_tag="ACG8OL_002138"
     CDS             complement(7405..7731)
                     /locus_tag="ACG8OL_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS4818111.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7744..9723)
                     /locus_tag="ACG8OL_002139"
     CDS             complement(7744..9723)
                     /locus_tag="ACG8OL_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4818112.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9744..10058)
                     /locus_tag="ACG8OL_002140"
     CDS             complement(9744..10058)
                     /locus_tag="ACG8OL_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS4818113.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10226..10519
                     /locus_tag="ACG8OL_002141"
     CDS             10226..10519
                     /locus_tag="ACG8OL_002141"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS4818114.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10597..11709)
                     /locus_tag="ACG8OL_002142"
     CDS             complement(10597..11709)
                     /locus_tag="ACG8OL_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4818115.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11862..12710
                     /locus_tag="ACG8OL_002143"
                     /pseudo
     CDS             11862..12710
                     /locus_tag="ACG8OL_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13063..>13953)
                     /locus_tag="ACG8OL_002144"
     CDS             complement(13063..>13953)
                     /locus_tag="ACG8OL_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS4818116.1"
                     /translation="LTTGEDGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERS
                     QTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKL
                     AIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKI
                     DDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYEL
                     DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFIL
                     SILGVLLVFISIKFRKKRNI"
BASE COUNT         4345 a         2853 c         2391 g         4364 t
ORIGIN      
        1 ctagccaaga acccatctgc tgaaatatga aaatttttga atgctacgac attcgccatt
       61 acaggtatgt aaaagaaaaa aatcatccaa agcatacccg gtacagccat caggacaagt
      121 gctttataac gccatagatt cataaaaaaa tgattgaacc cctttcgttt tttcatctaa
      181 aaaccccttt cgattcattc gcaacgtaat tatagcgctt tcaataatga gaataaataa
      241 acaaagtata gggaaaatga aacaaagtat aggagaggtt gtgaaatcag cgttttggcc
      301 tgagtattag agaaaaaaac gggaaaatag ttcctcatat tttgccgttt tttgagctaa
      361 taccgagggt cagctgattg aacaccgttt atcagaggtt gtgaattcag cgttttggcc
      421 tgagtattag ggagatcgtg tccttactat ctaactctga gtcacaagga acaatgggga
      481 ctgtaacaaa agtcttgtca cagtccctta ttccgaacaa acggtgttcc agaagtagct
      541 tctacggaaa taagccgaaa tttcacaaaa aagccatccg aaagtcagac ggacaagtct
      601 gcttttcaga tggcgctaca actactctgc ttattctttt agctatccat tcaattattt
      661 ttctctcact tgttctagct cttctttggt catgggtcga tattgccctt tattcaaatc
      721 tttaggcagc caaaattttc ccatacggat tctttttaaa tataagactt cttttcctac
      781 tgctttgacc attcgcttaa cttggtgaaa ctttccttca tgcaaaatca gacggataat
      841 tgaagtttct gtctcttgat ctacttgatc aatttggagt tttgccggca atgtctgctc
      901 atcgccatca ataatcagtc cttctgcaaa taaacggata tcttcttctg tcattatacc
      961 gctaacctca gctagatatt ctttctccac atgtttttta ggtgatagta actgatgggc
     1021 aaacaccccg tcattcgtta aaatcaagag gccctctgtg tctttatcta atcggccgac
     1081 tggaaaaaga tcttcccgat aatcctggtc ttccaataaa tcgattacgg tttgatcatg
     1141 attatcctga gtagcagaaa tgactcctgc aggtttatgc aaaatgtagt aaaaatcttt
     1201 ttgatacgta atgatttctc ctaaataagt aaccgcatct tcgaattcat ttacttgcgc
     1261 tttatcgctt ttcaccactt gtccattgac ttcgatcgtt ccttttttga ttaatccttt
     1321 gacttctttg cggctgccaa gtcccatatc tgctaaaaac ttatctaaac gcatgcgttt
     1381 cccccatttc aaaagagcat caatctagct cttctgattc acttatttta tttttaattt
     1441 atgtcgtaaa cgcgctattc ctggtcctag tagtctttct gccaagcgga ttttcaaagc
     1501 taaataacca tacactaaag cccctatacc tgctactaca agaatcaaca atagagactg
     1561 gaatttgcta tcttcgttta aaaaagcacc aaaaatcatc tttgtgatcc acgaagccaa
     1621 aagcataatc aaggtaagaa taaagatcag caatgtcctg cgccagacaa aacttggttt
     1681 aaagcgggct acttcatgga ttctctttaa gatcaaggta caagacacag caaatccgat
     1741 cattgtcgcc aataaagggc cgtatacttc aaataaacga atggctgggt attgaagaag
     1801 gagtttcact agaaacccaa taaccaaata attaattgcc gccttgtttt caaacattcc
     1861 ttgcagcatg ttggaaacta gcatatatag tcctaggaag agtcctacaa aacttgcttg
     1921 gatcagtaca ttacttccta gagaatccgg tgtataaaat aatgtattta gcggataggc
     1981 aagtaacata acaccaaatg tcgccggaaa cataaaaaat gaaaacaact gtaaatttcc
     2041 gcttgtcaat tttgccagac ctactcgatc tttcaacgta acagcttctg taatcaacgg
     2101 taacccagta gacgcgatcg atgttgccaa tgcaataact accatcgtca acttatctgg
     2161 attggcacta aaaatcgaaa aaagatccag taactgtgca ttggaatact cggtggatgc
     2221 agacatgatc ttcataaaag tgaactgatc gactaatttg aagatcgtta cccctgaacc
     2281 cacaataata aacggaatcg cttctttaac ggtatctaac aacaattcct tcgttgcaat
     2341 atttattttg ttttcgctgt agtgaataaa cgccgatgta tacgcttgat gtttcttcaa
     2401 aaaatacaaa aggacaagaa tacttgctag cataccgata aatgccgcga aagttgattg
     2461 cgtgaccgcc gtcacatagt caccatccat gactttcatg atgatgaatg tagccaatag
     2521 catataaaaa atacgtgcca cctgttctac aatctgcgac agcgcataag gcatcatttc
     2581 ttgattgcct tgcaaatacc ctcgtatcac actcatacaa ggaaaaacca atacagcgat
     2641 actcaaagaa cgcatcgttg gaatcagttc ttctccgcca ccagaagcat gagctagcca
     2701 aggagaggcg atgtacatga acaaagcaaa caacaggcct aacccgccca tcatctgtaa
     2761 tgcacggata aataatcgtc gtgaagtgcc atattcattc aatgaattat agcgtgcagt
     2821 ttgttttgca atcgcagcag gtattcctgc tgtagagatc agtagaaaca atgcatatat
     2881 attgtatccc atattgaata atccattggc agccttagca ttttcgccca tccaagcata
     2941 ccagggaatg atatagacag cgcctagcaa tcttgaaatg atattactgg cggtcatcca
     3001 agcagagcct cgtgccattt tttcttgcgg tgttaattga tctaatgctg gttttctttg
     3061 attatccatc ggtcagattc caactttcta caaaacatta ctatcatttt aattggtaaa
     3121 acagaaactg gcaatcgatt ttatataact tttataagaa caattgtttt tttgaattcg
     3181 gtttcgtttt tttggtaaag taaagaaata tacagccttt tatggctaac atttatagga
     3241 gatatcaatg acacaagaaa taaaaaaact acccgttata tttttaggtc ttttctttat
     3301 cacagttgga ttgaattggt tcttgctccc tcacgatata gcttccgccg gagtgggcgc
     3361 catcggacat ttagttgaaa cggccttttc tatccaacgc tcagttactg tttggagcat
     3421 caatcttatg atgctgcttc tagccgctgt cctattagga aaatcagtat tttttaagac
     3481 tgtagcggga agtctgcttt ttcctgttgt tttagagatt gttccaaaag tggaaatgat
     3541 ctcctcacat ttcttttcac tgatttcagg aagtcttttg ttcagtcttg gggtttacac
     3601 attgtacaca gtcggggcct caaatggcgg tataacgata ccgccgatta ttttccacaa
     3661 attttttcgg cttccaatgc cgcaaggact tttacttacc aatagtctta tcgtcttttt
     3721 gaattattat gtttttggat tgttggagac actattcgtt ctcctatcta ttacagtcag
     3781 ttcattgttt atgaaaatac tcatccgctt gtcccctgtg tcaagaaaat ttgaataaag
     3841 ctggattttg tcatagcgga aacaaacgat atattaaaaa gagtgtgcta taataaacca
     3901 agaaattgaa taaagcgagg gatcaatcaa tgagcttctc tttagaagac atccgtcatt
     3961 tactattaaa agaaaatcta ttaaaagaat ttgtttcaac aagaggctgg catcttgatg
     4021 taccagaaaa agctgaattt cttcagttaa gttatgattc aagaaaagct gatgcttcta
     4081 ctttgttttt ctgtaaagga atgaatttca aagaagaata tttggattca gcaatcgaac
     4141 agggtattca atattatgta tcagaacaac catacgaaaa ttcagcagta ggaatcatcg
     4201 taacggatat ccgtaaagca atggcgcttc ttgctatggc tttttacgac tatccgcaaa
     4261 ataaattgaa agtgatcgga ttcactggaa caaaaggtaa aacaactgct gcgtacttta
     4321 caaaagcaat cttggatcac acaacaaata aaaaaacagc attgctttct actatgaata
     4381 caacgttaga tggcaaaaca tatttcaaat cccatttgac aacgcctgag tcattagatt
     4441 tatatcgtat gatgtcagaa gcagttgaaa atggtatgac tcatctagtg atggaagttt
     4501 cttcacaagc ttacaaaaca caacgcgtat acggcttgac tttagatgtc ggaatcttct
     4561 tgaatatctc gcctgaccat atcagtccga tcgaacatcc gacatttgat gactatttct
     4621 attgcaagcg acaattgatc ttgaattcaa aaacagttgt tttaaatcat gaaagtgact
     4681 attttgattt acttagggaa acagccgatc tattccagat tccaacgatc acttatggtc
     4741 gttcggaaga aagcaattat caagtgatcc gttcagataa aggtactcac ggttttactt
     4801 tatcttctca tgaagatcgc ttagctatct gcgatactgc ttatgatatt ttgctggctg
     4861 gaggattcaa ccaagaaaat gcagccagtg cgatcattgc cgcagcacta acaggtgcct
     4921 ccagtcaaga tgcacaagac ggattaaaag aagctcgtgt accaggccga atggatcaat
     4981 tgatccaatc caatggagcg catgtctatg tggactatgc acataattat ctaagcttga
     5041 aaacattgct gaaatttgcc aaaaatgaac atccagatgg ccgtgtaatt gttgtattag
     5101 gcagcccagg aaacaaagca atctcccgtc gacatgattt tggaaaagtg ctatcagaaa
     5161 cagctgacat cgcctttttg acggcagatg acccagcatt tgaagatccg aaaaaaatcg
     5221 cggaagaaat caatgaagca attactaatc cagatttgat cgttcattat gaaatggatc
     5281 gtcctgaagc gattcgacgc gctttagcag aaagcgctcc gcaagattca gtcgttattg
     5341 cgggaaaagg cgtggatccc tatcaaaaaa tcaatggtgt agatgaacct tatgaagggg
     5401 attatgcaat cgtgaagcgg ttgattgaag aataatggta tttatatact agtggtttcc
     5461 catttctaag aaaaaaaggg actgtgattt tttgtcacag ttcctttttt cttagaaatc
     5521 attttaactt ttcactagct aattctgctg ccttttctct ttcttcattt aataatttat
     5581 tatcatagaa tttgataaat gggaaccaga taatgaatgc gatgattgca caaatgactg
     5641 ctaacacagc agctttccaa tcaccaccac taccaacaaa tgctccgatc ccgactggtg
     5701 aaggccacgg catctgtgca agcatcggtt tgacgatttc cagccgaatg gcaaaataag
     5761 cgagtgtagc agaagccatc ggagctaaga aaaacggaac cgctaaatag ggattgtaga
     5821 cgattggcat cccgaaaatg attggttcat tgatattgaa taatcctggt acaagtgagg
     5881 cttttcctaa cactttcagc tgatcagatt tcgcgaggaa tgcaataaag atgatcaatc
     5941 ctaaagtcgc acctgatcca ccaacggtta cataagaatt gttgaattct cctgccaacg
     6001 gataatttgc gccttgtgcg ttagctgcca tattagctaa tacgatcggg gtcaagaaag
     6061 aagtgatgat attcgctcca tggatcccta cgatccacaa tgcatggatc aagaaataaa
     6121 tcaccatgac accaagccat gtattcgtca actgcgtaac aaacccaaat gggatagcaa
     6181 tcattttgaa gatatccgtt cctagtgcaa ctaataatcc attgatgatc atcaccacaa
     6241 acgcaatgac aaatgttggg atcagagcag tgaaagaacg agaaactcct ggcggaacaa
     6301 catctggcat ttttatgacc caatttctct tgacacacat acaatacaat tcgaccgcaa
     6361 taatcgacat aataatagct gtaaagattc ctgaagttcc taaacgttcg acaaaagagc
     6421 ccatcctaat accatttact acagtttctg tctcattgat agaagcaatc aagctgattt
     6481 taccatcagt catgatcaat tcagggatac acataaagaa tgcaaaaaca gatagtaatg
     6541 cccccgtgag cggattaaca tttaattttt gttcatctcg ttcaattgac gttaattcat
     6601 aagcaaacac aatggcaaag taaatagata aaatccccat cgtcattgta ttgacgatca
     6661 tgtacaagtc actaactttg aaaaaagttg cattaaaaaa tccttctaaa aatgggaacg
     6721 gtaatggcaa aatatttaaa accaaaaaca tcgagcctac aatcgtgaaa ggaatagaag
     6781 ccatccccgc tgccatgaca gcccgaacga ttttaagttg cgcaattttc cccataggtc
     6841 ccatcaggta tttctgcaaa aaatcaaaca ttagttattc tcctttccta aatataacgc
     6901 tttttcatat tgtttaatac ggatatactg ttggtcttgg ttgagtgaaa ttttctttaa
     6961 acgatactgt ataaatgcac tggcaaaaat accgcttaca acaagaatga aaacaaataa
     7021 ctgtcctcca ttgttatttg ccataaaagg atacaagaat gtaaatcctg aggtaaacaa
     7081 cactggaatc actaataaat taaagacact ctggaacaaa aaataccact ttgtccatgg
     7141 aacgttcgtt tggtgttttc gataaagctt tacttgttct cctactgcag gcaaaatagc
     7201 taaaatcaga atcatcggta aactacttcc taaactttta gctgatagaa gcagaatcat
     7261 ccattgcatg ttcatgaaaa agaaaaaggc agtcaggtaa cgtacgatta gataacgatt
     7321 gaaatacatg tttttcaaac taagttgctt tttttctaat tcgattttcc tttttactgt
     7381 atcctgcaca atgatccttc ctttctattt atttttttga tacaaataat tcatttcctg
     7441 tgcaacttcc aataatgtca tcgtcgtcat cagatgatct tgtgcatgga ccatgataat
     7501 ttccatttct atttttgtcc cgctggcata ttctttcaga agttgagttt gtgactgatg
     7561 cgcatgaaga atctcttcat ttgcttgtcg caacttctct tctacttctt caaacatctc
     7621 tttacgcatc gagcgaaatg cttcatgtat cagtgtccgt gcatttccgc tatggaaaat
     7681 gatatcaaaa gaagtcactt gtagtgtttc actgtttatt ttttcagcca tctgttttcc
     7741 tccttattcg atcagtttga taaatttact gctgaaattc tcaaaaatag gttcagctaa
     7801 gatttcctgc tgtactttta gctggtcaac taacctaacg attgctttag tcacgaccgt
     7861 aattccttca ttttcgacat aagaaggcga aactaaaaaa acaaaatgaa tttcttgata
     7921 ttgatcccac tgcatcccag aaggaatcaa tgcaacggct acctttgttt cgattccaac
     7981 tggaacagcg ggatgtggga ctgccactgt atcactgaat atgatttgtc ccatattttc
     8041 acgttgctgg atctgctttc gcatttctct tatatatcct tcttcttcat ttatgctcag
     8101 cttttgcagc aaatcatgaa gaatttcttc ctttttcggt tcgtacgtat aagtcttgaa
     8161 aaatttttca gacaactgtt cggtgtaaat ctgctttttt tgctgtagag aaaatgctgg
     8221 tatcggtcta tctgaggaat gatagggtat agattctcca actacctttc gtattttacg
     8281 aacatcatct tcattcaaaa aaatactgac atgtaaaact ggaattttga aaaacattgt
     8341 cgaaagatca atagaagaaa taatcaggtc gattcctttt aaagttgttt cattgatctc
     8401 ataatagcct tttacactta ccactgtaat atttttaccg aattcactga ctacgcgatt
     8461 ctttaatagt tgggcactgc cgtaacccgt tgcacagata atcaatgcat gtagtttatg
     8521 cgcagctctt tctttttcca atgccgccat gagatgcaaa gccaaatagg cccattcatc
     8581 ttcattgatg gtatagtttt tcaaagccgg catgttgctg aaatgccttt ttactgcgtc
     8641 aaagactttg ggattttctt tctttatctc ctcagtcaat gggttctcaa tgcgaatacc
     8701 tcggctcaat cgaatcatca tcggtgacaa atgatcgagt agtccattga aaagctggag
     8761 atcctctact aatgaaaaat gaaattcctg agacaatctt ttcagaactt tcgctaattc
     8821 ctttgatagt tcctgatcat tttgattttc tttgtgattc gacttagcca tgagatgtaa
     8881 tgtcaaataa gcaatttctt cttcgggaaa cgatacacct gcgattacct caatacgttg
     8941 aacgatccgc tgcgccactt gatactctac tcgcttagaa acttcttgtt cgatgcctaa
     9001 ttccttgatt tcaaaccctt ctcggagccg tttgatcccc aaagccaagt gtaaagtcag
     9061 attctgtatg ataaaatccg aaacttttaa cttagcttct cttgtttcat cgagaatgat
     9121 aatgactagc tcttcaaaac tgatgtctgg gaaaaaacgg ctttgcccta aatattcttt
     9181 caatgaattt ccataatggt ttccaaaaaa agtatccatg ataaaatgcc gtcgatttct
     9241 ttcttcccct tcgatccatg tcccttgacc atgcttagaa acgattttta gtgaatatgg
     9301 aatcaatcgc tccttgattt cttgcatgtc ctttgtcaaa ctggaacgac tgatataaag
     9361 ctcttctgcc aaatcatcca taaaaagaat ggcatcttcc aaaagcaatt ggttcaatat
     9421 atacttttgt cgatcttcgg actcataaaa tccttgcggt tctctagcat atccaggtac
     9481 tatttcttgc cgactcaaaa acaagtcaaa tgtcagtctt tgtaaaatat gcagttggta
     9541 gccataacct ggtttagcaa tgatcttacc tccgtttttt tctactaact ctttgatccg
     9601 ctgcaagtaa ttacggattg tccgatcaga taataacaat tcagaagcta aacgttgact
     9661 ggtgacatac tccttcttgt gatcaatcaa atatctcaac agcaattttt ccttctgttt
     9721 cattatcatc cactcctatt ccatcatatg ttctcttcga tcacctctgc taacttctgg
     9781 atgcctgtag ggattgggac atacgcttgg aaagggatac taacaacagg ctttccaaca
     9841 gattctccta attttttgaa tttatccaaa aacatcgtcg tttgcgggct gatcagaaat
     9901 aaatcaaaat cactattctt aatcatatta tcgccttctg tagctgaaac tgcatctacg
     9961 agtacctctt ctcttttttg cttaaagaaa tccgttgtct ttgaggccat catcgaggat
    10021 gacattcctg ccgcacaaat aatcaatgct tttttcataa atcaatcact ccttttattt
    10081 ttataaacca agcatatctt gtaagcgttt tctattcaaa atatatattt ccgtagcatt
    10141 gcggaaaaaa tgtgtatcat atcttgaata ataatttctg gcaccgtgat atactagtga
    10201 tagaattttt tcaggagggg atttagtgag tatcacagtg aatattttgt atacaagtac
    10261 aaatggacaa gccagaaaat ttgcagaaga aatggtcgaa agaaacattg tcaaagcgat
    10321 tcgtgcagaa gaaggaaacg agaaatatga ctattacttt ccactagaag atccagaatc
    10381 tcttttactg atcgaccgtt ggaaagatga agaagcaatc gaaaagcatc acaaatccga
    10441 aatgatggct caaataaacg aactccgtaa aaagtataaa ttaaaaatga aagtcgaacg
    10501 tttcactgat ttaccgtaaa aagatacaca tggaatgata gcaatttatc atccatgtgt
    10561 atcttcactt tttccttatt ttttattcga tctcatctaa tgattcaaac cgtgtttttc
    10621 tcttattttt cggataagta tttcagtcac cccaatatac atgattccta aacatacccc
    10681 aaggatattc tcaaaaatgc ggatcgttac tgctcctgga accccgaata aactggcagt
    10741 gattgccaaa gcaccaaaag aattaaaaac tgttttatat ctataggtag aacaaacacc
    10801 taaagctaga ccacctaata taccgaccca gttaaaagga ataaattggc tgataccaat
    10861 aaataaaaca catccaatca atgagccaat gattcggtct actgctcgtt ctttgaaacc
    10921 aatcgacatc aatccatagc tagatacaat agaagaaaat gcaaaccctg cccacatcaa
    10981 tcgctgaaaa gggacatact ctcctgcaac gatcaaaaga ctaatgccaa atgcatagat
    11041 gagcatccaa attttttcct gcgaatacat gcctttcaaa aaaatctctt taaacaagct
    11101 tttgtctttg ttcttttctc gatgttttct gtaaaggaga actgaaaacc aacaaaaaaa
    11161 caaaaataat aagctacttg tttgtgtaaa gaaattcttg ttcaacgaat ctttaggtaa
    11221 cgaataaaca ataaagagat atgaaaagcc gtacaaagaa gcattcccca ttttgggatc
    11281 actagcggtt gccattaata tgctgcttag taaaataaaa tgaatcaaca tgcccataac
    11341 tgaccatttg atcagctgga catacggtgc tactaatagt gagaacatga cgattgctaa
    11401 acctatgatc gaatgggaaa tcttataacc aaagtcaaca aaacgaatac tcatcaacat
    11461 acagaataaa accacagcgt atggtttatg cttcgattca aataatgttg taattataat
    11521 gataaaacaa atggcaaaca atacgatgaa taatgatcgt aaaaacaatg aacaaatata
    11581 catgcacttt tcttttttag aagctgcttc tcgtattttt tgttttaaaa taaacggatc
    11641 tagttgtaga gactgataaa aatccacctg ttgtcgtcct ttctttttac ttttattttt
    11701 tacagtcatc tatgacaggt tgtaaatact atggaacatc atatggcgtt gtcattttct
    11761 tgtcaactat ttttcctatt catgttttta taaaaaaaca tatcgttttc ttgtatgatt
    11821 aaatcaccac aaaataaaca actgagagaa cgatatgctt tatgaatggt tctatcaaaa
    11881 aaattctgag atcaatagac aaaaatttga tgattacaga ggtctcttac gaaaccttac
    11941 agaagaaaaa aacattgatc gttgctgcag ttctcttccc tgctccatgt gtctgtagaa
    12001 attgtggttc tgctgtggta gatggaaata ggaaattaat ggtggtgaaa aacaagaaga
    12061 aggaaaccat tgtccacttt gaataatata atcatatgcc aatggttata cgcctaaaaa
    12121 agcagcgcta tacctgtaaa aactgtcgaa gccattggaa cgctcaaagt tattttatcc
    12181 gtccaaggca ctcgatttca aatcatgtca gacataaaat cacttcttta ctgactgaaa
    12241 aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc tttgacaacc gttattcgca
    12301 cattgaaaga gttgagaagc tatttaccaa agtcttccaa aagaattctg tcttgagtgt
    12361 tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat aaactgagtt ttatttccgc
    12421 ggatggcgag acagaaaaat taatcgagaa aatttaccta cacaaaaaat tcctcgatta
    12481 acaaactatt tccttagctg cactaatcct aaagaaatag aattcttagt tacagacatg
    12541 aacgccacct actttcagct caccaaacat gttctaccaa atgcgaaatt ggtgattgat
    12601 cgattacttg gtttttccac gcctttcaag gaggcgtaac ctttattcac gagttggttg
    12661 aagctttccg agacaaaaga cccgacttgt ttttctcctt attgacaaag agccattaaa
    12721 actagagaaa aaattacaac tgatcgctga ttatttgtag aaagaaaatc aaagtattac
    12781 ccaataaact tgagtcatta ggcaaaaaac actgcgaaac tcaagaatga ttttcgcagt
    12841 gtttttgtat attttttatt aactagtatt actacaaagg tttatttttc acttctcaac
    12901 gcagcacaaa attgtcttta cttatttact ggttgggttc aaccttctct actccgatac
    12961 taatatttgt ttgtataccc cttctaaaat cggtagtttc cattcaattg atgggtacta
    13021 gttgatttta aaaactatgc ttattcaatt ttttatttta gctcatatat ttcgtttctt
    13081 tctaaatttt atactgatga aaacaagtag cacacccaat attgacaata taaactggct
    13141 aatgatggtt tctccagttt ttggaagatg cttatttcca ctgcttgttg gattgtttcc
    13201 tcttatatct gagctatcag caacttttct attctctttt gttaaaaaga tcgccacttc
    13261 ttgttctttt tcgatcttga aagttactgg tgtcgcatcc aattcatacc ccgttggcgc
    13321 ttgagtttct accagctgat atgctcctgg tgctaagcca tctatcgcca gttttccatc
    13381 tgctcctgtg gttagacctg actgcagaac ggttccattg gcatcttgta actcaaaaac
    13441 agctccttgc aggacttctc cgctttggtc atcgatcttt gtcaacacga caccgcctgg
    13501 tgtaagtcga ttttctttcg tcaattctac caccgctgtc tgacttcttt cgatctcaaa
    13561 ctcgattggt gtcgcatcca attcataccc cgttggcgct tgggtttcta ccagctgata
    13621 cactcctggt gctaagccgt caatcgccag ttttccatct gctcctgtgg ttagacctga
    13681 ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca gctccttgca ggacttcccc
    13741 gctttggtca tcgatctttg tcaacacgac accgcctggt gtaagtcggt ttgctttcgt
    13801 caattctacc accgctgtct gacttctttc gatctcaaac tcgatcggtg tcgcatctaa
    13861 ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctggtg ctaaaccgtc
    13921 aattgccagt tttccatctt ctcctgtagt tag
//
LOCUS       DAZUJO010000069        13948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000069 DAZUJO010000000
VERSION     DAZUJO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.070"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG8OL_002145"
     CDS             136..309
                     /locus_tag="ACG8OL_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818117.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ACG8OL_002146"
     CDS             949..1422
                     /locus_tag="ACG8OL_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS4818118.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ACG8OL_002147"
     CDS             1431..1658
                     /locus_tag="ACG8OL_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818119.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ACG8OL_002148"
     CDS             2294..2665
                     /locus_tag="ACG8OL_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818120.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ACG8OL_002149"
     CDS             complement(2921..3163)
                     /locus_tag="ACG8OL_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4818121.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ACG8OL_002150"
     CDS             complement(3195..4097)
                     /locus_tag="ACG8OL_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4818122.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ACG8OL_002151"
     CDS             complement(4110..4298)
                     /locus_tag="ACG8OL_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4818123.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG8OL_002152"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ACG8OL_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4818124.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ACG8OL_002153"
     CDS             complement(4913..5806)
                     /locus_tag="ACG8OL_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4818125.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="ACG8OL_002154"
     CDS             complement(5884..6807)
                     /locus_tag="ACG8OL_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS4818126.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="ACG8OL_002155"
     CDS             complement(6856..7206)
                     /locus_tag="ACG8OL_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4818127.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="ACG8OL_002156"
     CDS             complement(7239..8141)
                     /locus_tag="ACG8OL_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4818128.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="ACG8OL_002157"
     CDS             complement(8134..8991)
                     /locus_tag="ACG8OL_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS4818129.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="ACG8OL_002158"
     CDS             9325..10134
                     /locus_tag="ACG8OL_002158"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4818130.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="ACG8OL_002159"
     CDS             10174..10671
                     /locus_tag="ACG8OL_002159"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS4818131.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="ACG8OL_002160"
     tRNA            complement(11056..11129)
                     /locus_tag="ACG8OL_002160"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="ACG8OL_002161"
     CDS             11316..11639
                     /locus_tag="ACG8OL_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818132.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="ACG8OL_002162"
     CDS             11803..12057
                     /locus_tag="ACG8OL_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818133.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="ACG8OL_002163"
     CDS             12127..12372
                     /locus_tag="ACG8OL_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4818134.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="ACG8OL_002164"
     CDS             12448..12813
                     /locus_tag="ACG8OL_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS4818135.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="ACG8OL_002165"
     CDS             complement(12874..13437)
                     /locus_tag="ACG8OL_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818136.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4090 a         2577 c         2304 g         4977 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atgaattt
//
LOCUS       DAZUJO010000070        13904 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000070 DAZUJO010000000
VERSION     DAZUJO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.071"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..2292
                     /gene="acm"
                     /locus_tag="ACG8OL_002166"
     CDS             127..2292
                     /gene="acm"
                     /locus_tag="ACG8OL_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS4818137.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2564..3049)
                     /locus_tag="ACG8OL_002167"
     CDS             complement(2564..3049)
                     /locus_tag="ACG8OL_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS4818138.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3139..3484
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3529..4170
                     /locus_tag="ACG8OL_002168"
     CDS             3529..4170
                     /locus_tag="ACG8OL_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS4818139.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4189..4824
                     /locus_tag="ACG8OL_002169"
     CDS             4189..4824
                     /locus_tag="ACG8OL_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS4818140.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4842..5657
                     /locus_tag="ACG8OL_002170"
     CDS             4842..5657
                     /locus_tag="ACG8OL_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4818141.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5976..7412
                     /locus_tag="ACG8OL_002171"
     CDS             5976..7412
                     /locus_tag="ACG8OL_002171"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4818142.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7601..10048
                     /locus_tag="ACG8OL_002172"
     CDS             7601..10048
                     /locus_tag="ACG8OL_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4818143.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10278..11345
                     /gene="rlmN"
                     /locus_tag="ACG8OL_002173"
     CDS             10278..11345
                     /gene="rlmN"
                     /locus_tag="ACG8OL_002173"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4818144.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11335..11541
                     /locus_tag="ACG8OL_002174"
     CDS             11335..11541
                     /locus_tag="ACG8OL_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818145.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11538..12641)
                     /locus_tag="ACG8OL_002175"
     CDS             complement(11538..12641)
                     /locus_tag="ACG8OL_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4818146.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13193..13741
                     /gene="aac(6')"
                     /locus_tag="ACG8OL_002176"
     CDS             13193..13741
                     /gene="aac(6')"
                     /locus_tag="ACG8OL_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4818147.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4762 a         2304 c         2951 g         3887 t
ORIGIN      
        1 ttgtaaaata actatcaatt ttttagttaa aagattggaa atgagtgggg gaggaattga
       61 agaaaggatt tttgagagat gatatagtag gttttatttt tagttaaaat aggggagata
      121 gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat tactaatata
      181 acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag cagtaatgtc
      241 acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa agtaaaattc
      301 tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat caattggcca
      361 agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat gattgaaaat
      421 aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt taatgaaaat
      481 attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc cagaaacgta
      541 accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga taaaacagct
      601 actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta ttataaaaca
      661 ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat taacaataac
      721 ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg acaaagatta
      781 gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt ttatagagga
      841 gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa tttttcagta
      901 actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag gaaaataatg
      961 gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa tcattcggaa
     1021 gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa tcatacagtt
     1081 aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt gaagattttc
     1141 aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt aagacgagca
     1201 gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga acagggagaa
     1261 gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga agcacccaat
     1321 tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa cgaaaatgat
     1381 acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat cacagcaact
     1441 aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt attcagagca
     1501 agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga caatggaata
     1561 acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga atatacgttt
     1621 aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta tcaaaaaatt
     1681 gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc aggacaaaaa
     1741 acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac agtaaatcta
     1801 ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga ttggcgctat
     1861 cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac tgtaacggaa
     1921 gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa cagttataca
     1981 ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa aaattttcca
     2041 gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca aatatttgct
     2101 catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc tgtacctaaa
     2161 cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa atctagtttt
     2221 gctgtaatca ttggtttatt gatagtaact atctgtgctg ggatattctt acaaaaatat
     2281 aaaaaagttt agttagaata taatattttt tgttaaatat gactgaaatc aaaagaataa
     2341 aattccgatt tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat
     2401 caataaatat ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata
     2461 ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa
     2521 gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa
     2581 ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg
     2641 tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga
     2701 tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg
     2761 ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt
     2821 ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat
     2881 agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg
     2941 aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac
     3001 ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc
     3061 ctcctttttt tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa
     3121 ttacacgtaa agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag
     3181 agagtctctg gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat
     3241 tttctgaagc gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga
     3301 tgaccagata aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat
     3361 taacgtcctt tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg
     3421 tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct
     3481 cttggcattt gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg
     3541 attgaagtta tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt
     3601 accttgattg gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt
     3661 aagccgctac gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg
     3721 ttgcaactga tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc
     3781 gaagcagcac tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt
     3841 ggagggatac aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt
     3901 ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct
     3961 gtagggaatg aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta
     4021 ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg
     4081 ctaggcgtgg caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa
     4141 gttgaaggaa aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg
     4201 aagaaagtga acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt
     4261 ccagatggga aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta
     4321 cgtatcttgg ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg
     4381 ttcaacccat tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc
     4441 cagctatttc cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg
     4501 aaacagacaa aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt
     4561 agtgatttat tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg
     4621 attgccagag cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct
     4681 ttagatcctg aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg
     4741 gtaacccaaa ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta
     4801 ctaaaagttt ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc
     4861 tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag
     4921 cagataattg ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat
     4981 ttgttcctat gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag
     5041 ccaaagcagt ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa
     5101 tgaaggaatt tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa
     5161 cgccggcaag agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc
     5221 tgatcacacc taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag
     5281 gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag
     5341 agatagtcaa aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc
     5401 tgaaaagcag acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga
     5461 aacaaagaaa cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt
     5521 ttgcagttgg tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag
     5581 atcaactaga gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg
     5641 ttacgccgag aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt
     5701 ccgaataaac agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac
     5761 aacctcttgg taaacggtgt tcaaaagcca gtttttcggt attaagccaa atttatgcaa
     5821 aaatgtagaa agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc
     5881 acaacctcct ttacataatt ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga
     5941 ggaacgattg aaggttatag gaaaggaaga gatgaatgga acacaaagaa cttacctcat
     6001 ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc
     6061 aagaagatgg gaaaggagaa tcagtctggg atcgatttgt acggatacct ggaaagacat
     6121 ttaaaggaac gaacggtgat ttggcagtag accactatca tcgttataaa gaagatattg
     6181 ctttaatgaa agaacaaggt ctgaaagctt accgcttctc tgtagcttgg acacggattt
     6241 ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc tttttatgag agattgatcg
     6301 acgaattgat tgaaaacgat attgaaccta tgttgacttt atatcattgg gatcttccac
     6361 aagcattgca agatgaatat aatggttggg aatcacgtca gatcattgaa gatttcactc
     6421 attatgcaga aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga
     6481 atgaacaaaa tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga
     6541 cagacccgaa acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca
     6601 tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc
     6661 caaattatcc aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc
     6721 tgatggccaa ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga
     6781 ggttcttaga agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag
     6841 aatctgctaa accagatttt cttgggatca attactatca aacggcgacg aatgcattca
     6901 atccattaaa cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa ggttcatcag
     6961 aagaaactgg agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa
     7021 attgggactg ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc
     7081 gttaccgcat gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag
     7141 aagataaaca aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca
     7201 tcaaagaagc aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag
     7261 atttgcttag ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag
     7321 acgaaacaca agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa
     7381 aactgattga agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg
     7441 ctggatatgg ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa
     7501 taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt
     7561 ttttttgtat aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc
     7621 tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat
     7681 gctgacgatt ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt
     7741 aggtttttta ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca
     7801 aatcctctgt cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt
     7861 ttcaattggt aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt
     7921 attgttacat gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac
     7981 gacatgggat ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg
     8041 tgggatgatt ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag
     8101 ttatttagca gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg
     8161 ctatcagctg ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg
     8221 tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc
     8281 agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag aagctgcaga
     8341 atatgagaag aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga
     8401 accagaaaat acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa
     8461 tatccatcct gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc
     8521 agaaggtttt tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga
     8581 agcagaacag gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc
     8641 aactgtggat caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca
     8701 aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt
     8761 cacaaaattc gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac
     8821 agatgatatc gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg
     8881 taaatcactg attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga
     8941 aatcatcgaa gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga
     9001 tgtttctgga cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg
     9061 ttcgactggt agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg
     9121 tgctaagccc catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat
     9181 gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca
     9241 agccttacaa aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg
     9301 agtaagaaat atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac
     9361 gaaacatcca accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat
     9421 ggtagccagc aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc
     9481 ggggattcat atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat
     9541 caaagcaaat gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac
     9601 gatcattgat tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc
     9661 aatgggtgag aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga
     9721 acgggtcgtt caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc
     9781 gacagatgaa gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga
     9841 agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt
     9901 tcggatcggt tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt
     9961 tggcccgtca gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga
    10021 aacgttgccg gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata
    10081 accaataaaa agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt
    10141 ctaatacggt atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt
    10201 atttgtgaag tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga
    10261 aaaagaggga aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa
    10321 tcaattggtt tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt
    10381 tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg
    10441 aaaaattatc ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt
    10501 cggatggtac agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac
    10561 tgatgagaca agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg
    10621 gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa
    10681 ttgttgcaca gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg
    10741 tttcacatgt cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg
    10801 actttttgca tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag
    10861 tttccactag tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca
    10921 atctggctat ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca
    10981 accgaagttt cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa
    11041 accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg
    11101 cgcaacaatt agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc
    11161 catataatcc agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga
    11221 gattttatga tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta
    11281 cagatattga tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca
    11341 aataatcgta tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag
    11401 gaactgttca acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg
    11461 acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg
    11521 ggggttttag caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca
    11581 tgataagttt ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc
    11641 acatgtttaa tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg
    11701 acattgagat tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag
    11761 attttatcaa acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg
    11821 acagatgctc cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat
    11881 gagtgtgcca tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta
    11941 acaatgatat ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct
    12001 ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt
    12061 cctaatctat ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct
    12121 ttggataaag catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt
    12181 gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg
    12241 ccttcttcgt tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg
    12301 ggaaaggtga agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca
    12361 cggccaccac cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac
    12421 tgttcaatat ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt
    12481 tcctgtgtca aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca
    12541 atcagcacta cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct
    12601 gttccaatgg tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt
    12661 cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta
    12721 gaggtgaacc taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc
    12781 aataagtatc ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt
    12841 gttcctctta gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa
    12901 aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta
    12961 aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct
    13021 ttcctgatct ttcctatttt gaataaagtg tttctgtata attttttatt ttataagtaa
    13081 gataaataat gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat
    13141 ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat
    13201 cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact
    13261 gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc
    13321 agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat
    13381 ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg
    13441 aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg
    13501 aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac
    13561 ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc
    13621 gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg
    13681 ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata
    13741 ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa
    13801 ttacttcttg aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac
    13861 cgtgtaccag aggttgtgaa aaagtcgctt agtttcgagt agta
//
LOCUS       DAZUJO010000071        13862 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000071 DAZUJO010000000
VERSION     DAZUJO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.072"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8OL_002177"
     CDS             168..362
                     /locus_tag="ACG8OL_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818148.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8OL_002178"
     CDS             631..930
                     /locus_tag="ACG8OL_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818149.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8OL_002179"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8OL_002179"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4818150.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8OL_002180"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8OL_002180"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS4818151.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8OL_002181"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8OL_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS4818152.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8OL_002182"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8OL_002182"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS4818153.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8OL_002183"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8OL_002183"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4818154.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8OL_002184"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8OL_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS4818155.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8OL_002185"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8OL_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4818156.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8OL_002186"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8OL_002186"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS4818157.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8OL_002187"
     CDS             7521..8291
                     /locus_tag="ACG8OL_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS4818158.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8OL_002188"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8OL_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818159.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="ACG8OL_002189"
     CDS             9959..11194
                     /locus_tag="ACG8OL_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4818160.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="ACG8OL_002190"
     CDS             complement(12193..12453)
                     /locus_tag="ACG8OL_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS4818161.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="ACG8OL_002191"
     CDS             complement(12480..13781)
                     /locus_tag="ACG8OL_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4818162.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4464 a         2833 c         2179 g         4386 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 ta
//
LOCUS       DAZUJO010000072        13694 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000072 DAZUJO010000000
VERSION     DAZUJO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.073"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..1524
                     /locus_tag="ACG8OL_002192"
     CDS             97..1524
                     /locus_tag="ACG8OL_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818163.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1616..1981)
                     /locus_tag="ACG8OL_002193"
     CDS             complement(1616..1981)
                     /locus_tag="ACG8OL_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4818164.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1978..2343)
                     /locus_tag="ACG8OL_002194"
     CDS             complement(1978..2343)
                     /locus_tag="ACG8OL_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS4818165.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2535..2783)
                     /locus_tag="ACG8OL_002195"
     CDS             complement(2535..2783)
                     /locus_tag="ACG8OL_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818166.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2782..2910
                     /locus_tag="ACG8OL_002196"
     CDS             2782..2910
                     /locus_tag="ACG8OL_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS4818167.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2967..4661
                     /locus_tag="ACG8OL_002197"
     CDS             2967..4661
                     /locus_tag="ACG8OL_002197"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS4818168.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4761..5057
                     /locus_tag="ACG8OL_002198"
     CDS             4761..5057
                     /locus_tag="ACG8OL_002198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818169.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5435..6568
                     /locus_tag="ACG8OL_002199"
     CDS             5435..6568
                     /locus_tag="ACG8OL_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS4818170.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6599..6919
                     /locus_tag="ACG8OL_002200"
     CDS             6599..6919
                     /locus_tag="ACG8OL_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS4818171.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6948..7412)
                     /locus_tag="ACG8OL_002201"
     CDS             complement(6948..7412)
                     /locus_tag="ACG8OL_002201"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4818172.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7543..8796)
                     /locus_tag="ACG8OL_002202"
     CDS             complement(7543..8796)
                     /locus_tag="ACG8OL_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4818173.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8825..9142)
                     /locus_tag="ACG8OL_002203"
     CDS             complement(8825..9142)
                     /locus_tag="ACG8OL_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS4818174.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9168..9488)
                     /locus_tag="ACG8OL_002204"
     CDS             complement(9168..9488)
                     /locus_tag="ACG8OL_002204"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4818175.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9952..11367)
                     /locus_tag="ACG8OL_002205"
     CDS             complement(9952..11367)
                     /locus_tag="ACG8OL_002205"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4818176.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11384..12700)
                     /locus_tag="ACG8OL_002206"
     CDS             complement(11384..12700)
                     /locus_tag="ACG8OL_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4818177.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12850..13548)
                     /locus_tag="ACG8OL_002207"
     CDS             complement(12850..13548)
                     /locus_tag="ACG8OL_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS4818178.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4348 a         2782 c         2357 g         4207 t
ORIGIN      
        1 atatgctttt tcagaaatta actgatgatt tcaaaaagaa aacatgtcat aatatttccc
       61 atattgtgca aaaaaataca aggcggaggc tctactatga ttgaagaata tattgagaaa
      121 aatattttaa gacagctgtt tttatgtggg caattctatg taaataaaga agtcaatcta
      181 gaaaagctct ccaatctcct gcatgtttgc aaaaccactt tactcaatga tatcaataac
      241 ataaaaaaag aatttgaaga acagatcgct tatacacatc gggaaaagga ccgttacact
      301 ttatattttt cagaacatat acctcgatgc aagatcatgc agcaattgtc tcaaaattcg
      361 ttgtttttaa aaacttgtct gctttatttg gaagaagatg aacctgatta tcttcaatta
      421 actgaatgtg aattcatctc tgtcagcaaa gcttattcat taaaaaaaca agtattagct
      481 tattttaatg actgcgggat cgaaatcgac cgctactccc ctcgattcac tgaaatggaa
      541 agacgtctgc tgcttcttaa tgtgtcgtat cgtttaggcg gttttaattc ttgggagcta
      601 cctgaatcgt tttttgaacg tgccgatcgt ttcattgaat ccgtaacgga aaatagtggc
      661 agattttatg ataaagaaaa taaagaaatc ttatcgattg gttttgctat cagtttccta
      721 cgccagcaag tgtgtgctgt gacaatagat tcaaaattta tcgaagaaat caaaaaacgt
      781 cctatttaca attatgtcga atcagcatgg gaaaacactg atttccaaac ctattataaa
      841 aaagaagaat tcgcctttat tttgacacta ttcaatcttt gtaattatgg tttccattct
      901 tatcagttga ttgcggaaga ctttcaacag ctacaccaag tgtttattga caatactccc
      961 gaaataaaag aactagttgc tacctttgaa tctcacttca atcaagaatt attcggaaac
     1021 caaccgttcg aacgtgcgct gattcatctg atgcgttcgg cttgggataa ctatcaatta
     1081 ttcatgccag aaaaatttta tcttttaaat gaggaacaga caaatttata taaagaagtc
     1141 cagacaattt tttccagctg gtccagccag ttaccttatg atctacggtt gaatcctaat
     1201 tgcatgcggg cttttgtgat cgaattgagc ggaattctcc gtctgacaaa agaacatctg
     1261 actatctata tcgtcacaaa ttcggatgtt cattatctga tttatcggga agccttggaa
     1321 gctgtaacta ctttcgattt tcaagttgca ccaacaattt attcaagtat atctgatata
     1381 aagaagtatg cgcagcagtc ctcaaaccgg gtattatgtg aacgtacatt atatacacca
     1441 gatgcggtcc aatatgaaaa tattattcct atttcgatca atacgatcga tcgagctatc
     1501 atttcagctg tgcaaaacaa ataaggggct gtgacaaaaa tcatgtcaca gcctcttatt
     1561 tgttgaagat gcccatttct gtaacaatct cttgatacac gtcaggctca caacctcact
     1621 tattttggac atctacgggt ccgatctctt tatttaaaaa ataagaaatg atggttcgga
     1681 taactaccaa cgccgctagt tggaaaatat cctgcaaggt aggtttgata atcgattcga
     1741 tgatcccagc tgaaacaaga atttccaaac ttagaagtat atagcctccc aacatctttt
     1801 taatagcatt gttctcgctg actcggtcgc tttgcacttt tgtatggaca aataaatatt
     1861 tcaaaaaatc cttgattgat agacaaacac cccaaaccaa taaacagata gcaaagatat
     1921 caagtcccag tacaagtttt tcgaaatatg gaagaagaaa ctgcatcaac tctgtcgtca
     1981 tcctacctct cctcttcttt gtttttcttt gtcttctatt tcctgattca agaaataaga
     2041 aatgactgtt cgaatgataa taataagtgc aagcttcaaa atatctgtcc aagtcggctt
     2101 gataactgac tcgatgatat cagcaacgac tagtacttcc aagcttaaca aaatataact
     2161 tgctagcagt tttcttgtat ctgcattacg agtattacgt tctgcatagt ttttcttgac
     2221 atgagaccaa tcaaaaagtt tgaatagaac aagaacgatc ccgacaatta agataccgat
     2281 agaacaaata tttaaaatag caatgatcca ttctagaaat tcaattaaat ttgagcgaaa
     2341 catatacgct acctactttc ttttaatttg ttcctattct tttattgtaa aagaaagtta
     2401 ccttgttcgt ctatttaaaa gttgttctaa tgataacggt tcgcttaaat ttttcttttt
     2461 cactctccca tttttctccc tccatccata aatcgtggaa gtatccaaaa caacgtagca
     2521 agttaaaata aggcttagtc cgtcatcagc ttttcaaaga agcactgtct cggtgctttt
     2581 tttgtaccca aatttctaaa aatagaacca tacacctcct caaatctttt cttcttacct
     2641 accctatttt tatttacagg aaaatcagac acttcttctt ttttgtcaaa atttcctttt
     2701 tcttctcgtc ctatcatttg gcattctaat aaaaacgaac tatccttgga tatagaaaga
     2761 gaaaacaagg aggcgttttt catgagtaaa aaattaatca aagtcggcat cggtttaggt
     2821 ttgcttgccc ttggcgctgc atatctagga aaaaagacag gactattcga agatgacagt
     2881 catttatatg atgaattcga atcaatctag catcctattt agttgaatca ctacggacat
     2941 acttggaaga cattggagga atcacaatga gaaaaagtaa agcaataatg attggtgccg
     3001 gtattgccaa tatggcagcg gctgtttatt tgattcaaga aggcaaatgg aaaggggatc
     3061 agatcacctt ttattcattg gatgatcacg gttcaaatga tggtgcaccg accgacactg
     3121 tagtggatga atattggaat aaaaaccatc cattagaaaa ccaaaaaggc tatattgctc
     3181 gaggcggtcg catgcttaat taccgtacct atgtagattt gatggatctg ttaagtcgaa
     3241 taccttctgt tactgagcca gggatgactg ctgaagaaga tacgagagat tttgatgcaa
     3301 aacaccgaac ctttgacaaa gcacgtttga tggaaggtgg caaaggaatc attgatggcg
     3361 gaaaactggg attcaataat aaagaccgta ttcttctgac taaactgatt gctatgcctg
     3421 attcagaaga agaaaaacta gacaatatca ctatcgaaga atatttcaaa gatgatccac
     3481 acttttttga aacaaacttc tggtttatgt gggaaacaac atttgctttc agaacacgta
     3541 gttctgctca agaattgcgt cgctatatgc atcaaatgat ttatgaattc actcaaatcg
     3601 aacatctagt gggcgtcaat cgaacacgtt acaatcaata cgaaagtatt atgcttcctt
     3661 tgatcgagta cttgaaagaa caaggatgca aaatcatttt gaaccgccgt gtcactgact
     3721 gggaattcaa agaaacaccg atgcaagatg aaatcactgt gactggtttg aaaattacca
     3781 atacactgac tcaagaagaa gaatacgttg tggtcgatga agatacagca gttattttca
     3841 ctaatgggtc cattacagat tcagctactt taggagatta cgaaacacca gctgtagaaa
     3901 atatggatta tggtgcagct tctagtctct ggaaaaaagc cagtgaacgt ttctacaact
     3961 tgggaaatcc tgacaaattc tttgctgatc gagatgcaag cgaatgggtc agctttaccc
     4021 tgacgacgaa aaaccatttg ttgttgaatg aaatcacccg tatcacgact caagaaccag
     4081 gaaatgcctt gaattcattt atttcaacca caccgatcac gccgttaggc caaaaagatg
     4141 tcaatatgtc tatcgtcgtg catcatcaac cgcattttac gtctcagaaa ccaaatgaaa
     4201 cagttatttg gggttatttc ctttatccaa gacgacgtgg cgaatttgtg gataaacagt
     4261 atattaaaat gaatggaaaa gaaatgctag aagaactgat tggacaactt tcaaaagtag
     4321 atcctggacc agtgaatatt cgtgaaaaag aaacagaaat tttcgacagc gtaataaata
     4381 atatccctgt ctatatgcct tatgcttccg cgttgttcaa taatcgtgcc aaaagcgatc
     4441 gaccaaaagt cattcctaaa cattcgacca atctggcctt tacaggagaa tttgtcgagc
     4501 agccttatca gatgatcttt actgaacaaa gtgctgtacg ttctggagaa atcgctgctt
     4561 tccattttgc gggtattcct atgtcccgtc ttgtcaaaac accgcgttac gacaaagata
     4621 ttcctactct agctcgtgcc gcaaaaaaaa tgtttgaata atcaagaggt tgtgaaaaag
     4681 ctgccctgca tcaagtaata agaacaacca ggctaaaaca gcttcttatg tttttgattt
     4741 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttgtgaaa
     4801 aagctgccct gcatcaagta ataagaacac ccaagctaaa actgcttctt atgtttttga
     4861 cttggttgga cttattaccg cagatgcaag cttttgaaca ccatttacca agaggttatg
     4921 aaaaagctgc cctgcatcaa gtaataagag cagccaagct aaaacagctt cttatgtttt
     4981 tgacttggtt agacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt
     5041 atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg ttcctcacgt
     5101 ttttgcataa atttgactta ataccgagaa actgatccct tctgtcataa cctcttttta
     5161 tttatgtctg tttctttttg aaaacaacta ttttttcaac gtttactctc ttgccaatcc
     5221 aacatcatct aacggataca actatttcaa tgattaccat tacactaaaa aatcggaaac
     5281 acacctctcc gaacacaaat acataagact atagaatgtt tcatttaaga aaaaaagcgt
     5341 ttcattatca aaaaggtaag atctccttta aaatgaaatt atctgtagta ctaataatgc
     5401 agacaatttt atgaatctga aaggagcatc tccaatgaaa gcagtcactt ggcaaggaaa
     5461 acagaaaatg gaaatcagaa acgtagatga tccaaaaata ttagaaccaa cagatgcaat
     5521 catccgtatc acagcaacag ccatttgcgg ctccgatctg catctttacc atcatggtga
     5581 agcagttttg gaaaaagggt atgttgtagg acacgaacca atgggaatcg ttgaagaagt
     5641 gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt ttaatattag
     5701 ttgtggtcat tgtcatttct gcctgaatca catggaaagc caatgtgatg aatcaaatcc
     5761 agatcagtta tatggcgggc ttttcggatt tggcaagttg aacggtaatc attggggtgg
     5821 tcaagcggaa tatctaagag ttccttatgc agactctact tcatttgttg taccagataa
     5881 tgatcttcct gatgaaaaag ttttatttct atcagatatt ttgcccacag cttattggag
     5941 cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg attctaggtt ctggtccagt
     6001 cggtctattc gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa tcgcagtaga
     6061 tccagtacag caccgtctag ataaagcggc taattacaat ggggttgaaa cttatttctt
     6121 agaagacacg gcacaagctg gaacagaatt gtatgaactg gctaaaggtg gcgcagatgt
     6181 cattatcgat tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag cgaaaaattt
     6241 agtaagctta caaagcggca caatctctcc attacagatg gcatcgcaag ctgtcaaaaa
     6301 atttggaaca gtccaagtaa ctggtgttta tatgactcca gcttcttctt acccgctgca
     6361 agaatttttt atgcgggata tcgctgtcaa gcatggacag gcaccaatta tccatctaat
     6421 gccaaaaatt tacgcaatga ttgcagaagg actattcgat cctacacaaa tcattactca
     6481 ttcgctgcca ctatcagaag cagcacaagc atatgagatc tttgacaaaa aagaagataa
     6541 aaatataaaa gttgttttga aaccttgaat tcagtaaaaa atacaggtga ttttctttat
     6601 gcccaaaatc aaacgaaata cccatgagat cgaaatagga aaagaagaag atatcgaaat
     6661 acgaggacgt cgcttccgct tctatttcca aaatcgttac acattgatct cactggctgt
     6721 cgatttatta accgggattt tctatatttt tggaagcctt gcttccttaa ctaaaatccc
     6781 cgatcgatat ggcacctatt tttatttggc tggtgctatt ttcttgacga ttcgtcctat
     6841 tctcagaatc gtaaggaaca tttttattta cgacgaaaaa aaagcaatga agagaacaag
     6901 caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc tcttttttca ccctatcgta
     6961 tcactttgat acagacgagc ggtcgtccca ctttcttcct tgaccattgt caaaaatgtc
     7021 cactcgtatc gttcataaaa cgtggtatga tctgtaatta agtaaagttt ttcgtaccca
     7081 agttgatgca tttcttttct gatttccgct aatatccgct tggctatccc ctgcttacgc
     7141 ttttccggtt caacaaacag ggcacaaaga tttggtgtca gatcagggcg gttatggaag
     7201 tcgttttcaa tgacgcccgc tcctccgacg atggtttctt tttcatctaa gaccacatac
     7261 cattgaggaa tactttcctt attagcgatt ccagtacgaa tactttcctc atacacaaca
     7321 gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt gttttactaa ttctgggtgt
     7381 tctcttaatt tgatccagtt atatgtaacc aatgtttaaa cctccgacta aaataaaacg
     7441 tttacaaaaa ttatacaagt ctttatgaaa attagcaatg acaacaatag tgatgtaaaa
     7501 aaaaaacgga aaacaatcac ttgttctccg ttttttgatt aatcaagcat ctaaagcttc
     7561 ttttcttctt tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag
     7621 aaccaaattg acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc
     7681 aataattggt ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga
     7741 agtcgcaaag taagcaacac taactaagac cattggtgtc aaaataaatg gaatgaacat
     7801 gatcgggttc aacacgatag gtaacccaaa catcgttggt tcattaatat tgaagacacc
     7861 tggcgcaatc gacaagtttg ttacaaccat atatggtttg tttttacgtc ctactaaata
     7921 aatagctaac agtaaaccta aagttgcacc tgttcctcct aagttgacaa agctgtcaaa
     7981 gaaaggtttg ttcacgataa acggtgcgct atgcccagct gcgattgcat taacgtttgc
     8041 ttcaatggct ggcgcattga ttgtttgcat caaaggatcg accatattcg ctccgtgtaa
     8101 accaaagaac cataagaatg gtgtgataaa tgcaagcagt aaggcagatg gataagagtt
     8161 tgcaagtccc ataaatggtt cttggaccac tgtatagaaa gaacctacga tatcatcaac
     8221 accgaatgca gcaaaaattg cagcaactaa agcaaacgca gagatagtga tcattgctgg
     8281 tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca ttttgatgat
     8341 caatttttca tttccgctta aacgtacaaa gatttctgtt gaaataatcc caacaataat
     8401 agcaataaac aaaccgtttg agctcatacc taatgctccg cctaaagcga agaaagagcc
     8461 taaagaaatc acacctgcgg aaacaccgtc ttttcggtag ccgttagcta aatgataagc
     8521 aagcaagaaa gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag cagcagcccc
     8581 gaatcctttc catgattcac caccaaaaat tgagttcatc aattcttgga acccgggaat
     8641 cggtaagtta ttgatcatta ctgctaatgc tcctaagatc attaacggca tactcaccat
     8701 aaatgagtcc cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg ctacaggtat
     8761 aaaatgtttc tccataaatg caacaaattt atccataaat cttccctctt ttctttactg
     8821 tagattacgc gttcaatcga cgatacagat caatgatctc ttttgctaaa tctgtaaaag
     8881 ctatagatgt catcagatga tcttgactgt ggaccgtcaa aagcgtgact tcaacgtgat
     8941 ttccttgtgc ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg actaaagatt
     9001 cctctgcatc tttgattttt tcatctgcta aagtgaaatc accttttttc gcagcagcaa
     9061 tcgcttccat tgcatcgctt tttgcatttc cgccaaacat gatcagaccc atgatggctt
     9121 ctagattttt ttgatcttcc atttattgta tcctccgata atttttatta tttgtttatt
     9181 agtttttctg cttggtcgag tactttttca ccattcatca tgccatagtc agccatattg
     9241 attacatcta aaggaatgcc tttaggtgct agcttttcag caaattgatt ttgcataaag
     9301 cgtacttgcg gtcctaaaag taaaacgtct acatttttag attctagttg attatccgca
     9361 tctgaagcag atacagcaaa gatgtctgct tccattcctt ttgcttctgc tgccttttgc
     9421 atttttgtga ctaggagact tgtactcatt ccagctgaac atacaagcat gattgttttc
     9481 ttttccattt taaacacgtt cctttctata cttacacttg tatataagca agtagcgtgc
     9541 caactttaaa agcgctttaa aaagttgata tcatcacatt taaggtctat accaagttgc
     9601 atacacacta aaaaagaaag cgcacactaa acacacaaat taatttttat tacctttttt
     9661 cttttaacgt gcacaaaaaa agttgtgaca caaaacttgt cacaactttt ttcaaataaa
     9721 cggtattcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cataagatgt
     9781 tgtttttact ttgctattct tattacttga tgcaggacag ctttttcaca acctctcaat
     9841 aaacggtgtt caatcagctg accctgggta ttagctcaaa acgctggatt cacaacctct
     9901 tttatttcaa ataaccagta aatacaatga acatctacaa aaaacttcta tttatttata
     9961 gatttcgtca ggcaaatcca attctctctt atcaatcaaa gtctgatacc acgaagcaga
    10021 ttttttcaat gttctgcttc gttcatcgtt caattcgatt cgaactaatc catatcgatt
    10081 cttaaatgca ttcattggtg acacacagtc agtgaatgcc cataacatat agccttcgca
    10141 atttgcgcct tcttctgttg ctttgatcaa gtaatgcaaa tgctgtccga taaattcgat
    10201 acggtaatca tcttgcacga ttcctttctc atccatatac tgttcttcat attctcttcc
    10261 cataccgttt tctgttatca gccaagggat attatgataa ttttctttta gatacatggc
    10321 tatatcatac atgattttcg gatagatttc ccatcccctg gaattattca ttttcttccc
    10381 aggtaaatca aagacttcat aatatcgttc aggatggaaa ggtgtctctt cattccattg
    10441 ataacgaggg gcgcgaacgc gtttaggata atattgattg attccaagga aatctactgt
    10501 atgatgctga atcgtttcta aatcctctgt tgcaggatcg aaataaatcg attgtttttg
    10561 acacaactcc aggaactctt ctgtgtagta tccttttacc atcggatcaa aaaatactcg
    10621 attataaaac aaatcataca tataagcagc tttttggtct tctggagaat tggatcgagc
    10681 atagaccatt tcaggattta aaatacagcc aatccttcca ccataatttc cgctgcggta
    10741 aatctctact gctttagccg ttgctaaaac tttatgataa ttccacatca tccacttttt
    10801 tgtgttttgt tcatgcggcc agcgaacagc atccaaatag caacgcgttt ggggaacgat
    10861 tggttcgttg aatgtaaacc agttttttac cctatcaccg taacgatcaa aggcaatttg
    10921 agcatactta gcataaaaat caactacttt tcttgaagac cagccatcat atttttccaa
    10981 aagaaaagct gggacttcat aatgttccaa acaaatcatc ggttctacgt ctgcttcgat
    11041 caaagagtcg atcatttcac ttatatgctg agcatattct tcatctacta tacagttctc
    11101 ataatcagta aaaaaacgag cccaattgat agaagtccga taatgtgtca gatggatgga
    11161 tttcatcaga tccacatcct gctggtattg ttccataaaa ttagttgcta cggctgggcc
    11221 atacccatta tgccaaacaa aatgttcatt tttataccag ctatctataa acgaatcctg
    11281 tccttctttt tttcctttcc aaccttccgt ttgccaagca gaagatgcag caccaagaat
    11341 aaaatttttt ggaatagtaa ttttcatgat ttcctcctgt tttttagatc ccatcatttt
    11401 ccacacgatt tgctactttt acaaatggca gatagatcaa aatagaaaca ataatacaaa
    11461 caagttgcgt aataactgca ccgatatttc ctgctgttgc cagataagca ttgacgatcg
    11521 gcggcattgg ccaagggacc attacaactg ctttcgccgc aaatcctaaa gcggttgcaa
    11581 aataaccaat cgttccagtg ataagaggcg ttaagataaa cggaatagct aaaattggat
    11641 tcagcatgat cggcatacca aaaataactg gttcattgat gttgaagatc ccaggagcaa
    11701 gggataactt ggtgatttgt ttcatatctt cccgttttcc aactaacaat actgcaatca
    11761 gtaaaccgat cgtgactcca gacccgccca tactcatata catatcccaa aatggcatag
    11821 taataatgtt cggtacttct tttccagctt caaaagcttc cgtatttaca gcaattgccg
    11881 ctaaaagcaa cggttcccgt atcggcttca ccatttgatt tccatggata ccaatcaccc
    11941 aaaagacttg cgcaataaac atcaatagca aaatacccgg taacccttgt acgacacctt
    12001 ctaatgggcg ttgtacgaca ttgtaaatga tatcataaag gtacattcct gtaagttctt
    12061 tgatgataaa tcccgcagcc gcgatacccg atacagtcag aatcgtcggg aataaagcag
    12121 aaaaactagc agaaacattc gttggcacgg tatcaggcat tctcagctgt agtttcttct
    12181 gtttcctcaa ccagcaataa agttcaatag aaaggatgga gaccaacatt cccaaaaata
    12241 accctttcgt atctgtatat tgtttagcca atacgttttc tactataaca gtctgatttt
    12301 cattcactaa ttcataaaca gttggattaa cagataaata agacatcact gcgatgatac
    12361 cagggaatac accatccact ttattcagct tggctaattc gatccctatc agaaaaacag
    12421 catagatggt caagaaactt agtgtgacat agctgatggc agatgcgata ggctttaatt
    12481 cttctaaaaa acgcaagaaa cttatctgtg ctaagccatt ttccgagtca aagaccattg
    12541 ctgaaaacaa tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt
    12601 tttttatcac catgatatac cttaaattat tgattctcgt tgccacgttc cctaaaacat
    12661 tggctaattt atccataaaa ctcattccag atcccctcat tcctgcttct tctccttgac
    12721 gtcatctctt tttgtctatt agtcagcgct tacatttcga tgaacagaaa ctatttactc
    12781 ctctttttta ccaaatataa aacaaaaatt catctgatgt atttatcata aaatacatca
    12841 gatgaactgt caattagttt ctttttctcc tattattttc tgtaattctc tccgatttat
    12901 tgccatatgg acagtcattg catcaaacgc agcaagtgca ttccgccttt cgatggcctg
    12961 aacgatctct gtatgaatat cgatcgtctc gattttcaga tgctggatat cataattttt
    13021 attaaacaaa gcaattgatt cattaatgat tgggataata tgcagcaagg ctacgtttcc
    13081 gctagcttct gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc
    13141 ttctcgaaaa gaagtttcaa tcttttgcgt taattttttc atttggacga tttgattctt
    13201 cgttgcatat tccgctgcca aacctgccat cttaggctcc aataaatatc tcagttcaaa
    13261 taagtctcgt atcatccgat cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc
    13321 aatccctcgc ttatcactga caaatgtccc cgctccttgc cgaacttcaa ggatattcct
    13381 ggaagctaga gcccgcacag cttctcgcac cgtgcttcgc cccacctcta aataatcgga
    13441 caattcatat tcattcggca gtttatctcc tacttgcaac tgcatttcct ttaaatatct
    13501 aaggatcttg tcagtagtta cttctactag ggttttattt tcttccaaac cacatacctc
    13561 cttttcatct gatgtatttt ttcgtaatta ttataacacg tttgaatcat ctgatgattt
    13621 tttgtataca aaattgatta atccgttcat agacatacaa acgaagaaga tatttaccat
    13681 aaaaaagact agga
//
LOCUS       DAZUJO010000073        13236 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000073 DAZUJO010000000
VERSION     DAZUJO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.074"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..1040
                     /locus_tag="ACG8OL_002208"
     CDS             171..1040
                     /locus_tag="ACG8OL_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4818179.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1249..2274
                     /locus_tag="ACG8OL_002209"
     CDS             1249..2274
                     /locus_tag="ACG8OL_002209"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS4818180.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2313..3263
                     /gene="manA"
                     /locus_tag="ACG8OL_002210"
     CDS             2313..3263
                     /gene="manA"
                     /locus_tag="ACG8OL_002210"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS4818181.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3357..5060
                     /locus_tag="ACG8OL_002211"
     CDS             3357..5060
                     /locus_tag="ACG8OL_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4818182.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5062..7095
                     /locus_tag="ACG8OL_002212"
     CDS             5062..7095
                     /locus_tag="ACG8OL_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS4818183.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7106..7546
                     /locus_tag="ACG8OL_002213"
     CDS             7106..7546
                     /locus_tag="ACG8OL_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4818184.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7746..8873)
                     /locus_tag="ACG8OL_002214"
     CDS             complement(7746..8873)
                     /locus_tag="ACG8OL_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818185.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8876..9553)
                     /locus_tag="ACG8OL_002215"
     CDS             complement(8876..9553)
                     /locus_tag="ACG8OL_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4818186.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9817..11583
                     /locus_tag="ACG8OL_002216"
     CDS             9817..11583
                     /locus_tag="ACG8OL_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS4818187.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11598..12035
                     /locus_tag="ACG8OL_002217"
     CDS             11598..12035
                     /locus_tag="ACG8OL_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4818188.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12048..13199
                     /locus_tag="ACG8OL_002218"
     CDS             12048..13199
                     /locus_tag="ACG8OL_002218"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS4818189.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4495 a         2300 c         2733 g         3708 t
ORIGIN      
        1 agaacagctt tttcacaacc tctttttata agagttttta ctcattaatt tacaaaaaac
       61 aagcaaaatg cgaattttct cacatttttt ttaacaaaat caaagttatt gcttgaaaga
      121 agaagaatcc atcgttaaaa taaagcttga cgtagagggg agcaagaaat atgaaagatt
      181 ttcaattggg ggctgccacc tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata
      241 aaatgattga tttttacaag aacgttgttg gctttgattt gaaaagagaa gaaaacgaat
      301 tagccatttt aggaacagaa gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta
      361 gagcgaatga tcacatgggg gtgatcaaaa agctgaaccg tttttcattg attatcccta
      421 gcacagaaga aatggcagat atcctttgtc gtatccgtaa aatgaattat ccaatcaaag
      481 aagctttaga agatcatgga cgtatgggga ttttgctcgt agacccagaa gagaatgaat
      541 tggaaattta ccatgaagag aataagaacg accaaagaga aattccagaa acaatggatc
      601 aagaagcatt attaagaaaa tctgaagaaa aattcagtaa actatcaaca ggttcttatt
      661 ttgaaaaaat ccatttaaat gttactaact taacaaaaga acgccaattt cttaatcatg
      721 tacttggttt gaaggtacat aatgaagaag agggcatcca tgtattgaat gaaggagatt
      781 tccatgttgg tttgacagaa gctagcggag gcgcaattga tttgcctaca gatgaagtga
      841 ttggacttga ttttctgaaa ttcatgatcg atactgaaag catggaagga ctgattgctc
      901 acttgcagca actatcagtt gatttcttct tagacaaaaa gcacacagta ctcacaatct
      961 atgatgctat aggaatcgaa tggtggttcg tcagtgagcc aaaagaaaag aataaaactg
     1021 acattcgatg cgacgcttaa ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt
     1081 atgaacggtc atactatcgc ttattctaga gatagaaaga gaaaatagta taccggggga
     1141 aactgcgcgg tttttcgcgc agtttttttg tgtaaaaaag gttgattcct catagattaa
     1201 gctttaagct tggactgtaa gaatctgata gaaaagcagg agaaaaagat gtatccacac
     1261 acgaaacagc ttattttgaa aaagatggaa gaaggatttt atccaggtgt agcagtcgcc
     1321 ttcataaaag aaaatcaagt agaaacattt acagccggct ttgcccaagt cgttcctgaa
     1381 aagcagctga tgacagaaga tctgttgttt gatgcagcct cactgacgaa agtcgttgca
     1441 accacttcat tagttttacg tctattagaa gatggaaaga tagaactcga tcaaccgctt
     1501 cataaatatc ttcctgaatt tgaaaatcag acaatcactt tgcgtcattt actgacacat
     1561 acttctgata tcaaaacatg gataccaaac agagataagc tatctcagga agaattgatc
     1621 gccgcttatt tgaagctaga accaggtgag caactaggaa aagaagtaaa atatacagat
     1681 gccggtacga ttcttcttgg gttgatgcta gaggaaattt accaagaaac tgcagtggaa
     1741 atctttcaag aacaagtatt ggaaccatta ggaatgatga acagcttatt tcttcctttt
     1801 ccttccaaaa aaatcgttcc cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa
     1861 actcatgatc cgaaagcaag agttttggct gaacatgcag gtaacgcggg tctctttatt
     1921 gatttgaaag acagcattac ttttgtccaa atgtatttga acaagggaaa aatccgtaat
     1981 ggtcgttttt tagaagaaga gacgattgat tctttactgc aagatcaaac accgaaaaaa
     2041 aatggctatc gttctttagg atgggactta aaaaaggatg tagaagacgg tcatgcgatt
     2101 ctttttcata ctggttatac aggtacgttt ttcttgattg atataaaaac acaagaagcg
     2161 ttgatatttt tgtcaaatcg cgtacatcct gtcgatcatc gtgcggagta tctcatatgt
     2221 cgagatgaaa ttgttgaaaa ctttttgaaa gagaaagcgg tattaccgaa ataatgctat
     2281 aatagctatg aaaaatgaga ggagtttgaa ttatgcagga acctatgttt ttaaaaccag
     2341 tttttcaaga aaaaatttgg ggaggaagtc gtttacgttc agtctttggt tttgatatcc
     2401 ctaacgataa aatcggagaa gattgggcga tcagtgcgca tcctcatggt gtcagtgtag
     2461 tagaaaatgg cgaattcaaa gggaaaagat tagatgaact atggtcagaa cacaaggaat
     2521 tgtttggcca tccttcagaa ccagtgtttc cattattgat caagatctta gacgcagaag
     2581 atgatctatc tgtacaagta catccagatg atgcctatgg gatgaaacat gaaggtgaat
     2641 taggaaaaac tgaatgctgg tatatcatcg atgctgaacc tggtgcagaa atcatctatg
     2701 gtcatcatgc aaagacaaga gaagaactag ctgagatgat caaagatggg cgctgggatg
     2761 accttttgaa gaaagtacca gttaaaaaag gtgatttctt ttatgtacca agcggaacga
     2821 tccatgctat tggtaaagga atcatgattt tagaaacaca gcaaagttct gacacgactt
     2881 accgcgtgta tgactacgac agaaaagatg cgcaaggaca aacaagagag ctgcatatcc
     2941 aacaatcgat cgatgtaaca actgtccctg cgaaaacacc agaattacaa atcaaagaag
     3001 tccgaaaagg gaattcatcg atcgtcactt atttagaaac tgagtttttc aatgtatatg
     3061 agtgggatat taaaggaatc acatctttca aaaaacaagc cccttataca ttgatgacgg
     3121 taatcgaagg agcaggagac cttgtcgttg acggaaaaac atatccatta gaaaaaggaa
     3181 caagctgtat tattccaaat ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca
     3241 ttgcgactga accaggcaaa tagttcccag atagctgaaa gcagcagacg gtttttcgtt
     3301 tgctgctttt tctacataaa atccgtccat caataaatag aagtatggtg aaaaagatgg
     3361 aatacaaatg gaaagcaaac aacagcagtc gcgtgtctat gaaacaactg agaaaagctg
     3421 gacatcactt aaatgaaatg atcatgccaa atctaagtct gtttatcgct tggggaatct
     3481 tgtcattagc aacaaaaggt gtgtctggcg atttgcacac cacattagaa gaggtatgcc
     3541 attggatgat ccagctgctt ttacccatct tgatcagtta ttcaggcggg aaatcggtag
     3601 ggggacaaaa aggggcagtc gccggtgcaa ttgcttcttt agggttgatc gctcgttcca
     3661 atgctccaca aatcgtagga gcgatgatta tcgggccgct tgccggcatc gtatatgaac
     3721 aatttgtttc tagattccac gtgaaattca aggctggata tgaaatgctt gtcagcaacc
     3781 tactagtagg actgactggt agcgttattt gtataattgg aatcgttttt attgaaccag
     3841 ttttaaatag tgttcacttg ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc
     3901 tgttaccact tgtcagtctg atcttagaac ctctaaaagt actatttttc aataatgcaa
     3961 tcaatcatgg tgtattgaca cctttaggaa tcgaattctc acaaacagtc gggtactctt
     4021 ccttattttt gatggaggca aatcctggac ctggtttagg gatcttactt tctatccttt
     4081 gttttgcaga aaaacaggaa aaagtaaatg cgagtggagc attgatgatc catgcgattg
     4141 gcgggattca cgaaatctat tttccatttg tcttgctgcg accgtatcta tttttagctg
     4201 taatggctgg tggggcaagc ggcacgttga tatttcaatg gttagatacg ggattagctg
     4261 cacctgtatc gcctgggtca ctactcgtga tttttgccaa tgcgccttat ccacaactag
     4321 ctggggtaag tatagggata gcaatcagta catttgtgac cttcatttgt gcagcgatac
     4381 tattgaaatt accaaataaa gaaaaagaga acaaaacggt caaagaaagg ggagaaatct
     4441 tggaaccatc tatcattcat tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg
     4501 ctatgggagc cgccctgctg aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg
     4561 gatacagctc tatctatgaa ttgaataacc aagtaaatca gctggttatc gtgcaacatg
     4621 aactagcggg tcttgcaaga gaaaaagtac ctcaaagtca aatctatgta ctggatcact
     4681 ttttggaaac agatatcttg ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg
     4741 aagaaaaaaa agagatagca gatatcacag aacaaaaaaa gtcgcctgct aatattgtat
     4801 ttttgtataa aaacaacaga cgcggctcac aaactatggg catgacgatt ttaagtcaat
     4861 tagcaaaaaa agcgaaaaaa cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa
     4921 tccaagaaga aacgatttat atcgcacaaa gagaactagc tgtaactgaa cagctagaag
     4981 aaaagatccc tcggctagtt ctagttgaac attgggtagc tacgaatgaa tacgaaaaac
     5041 tcgtgaaggg agaaatataa gatgtattta tcgcccagag aaaaacaatt attagcagaa
     5101 tttctcagta gcccgtcacc tgtatctatc caaaaaatga tgaatttatt gaaggtcagt
     5161 aaacggacta tttaccgcga attagataat cttgaagtct cattgaaatc ggtaaatgct
     5221 tctttgaaaa aagcagctag aggctcattt gtcattgagg catcagaaga agtattagag
     5281 caattaaaag aaaatacaga tgggaaaagt aatgacttat ccactgctaa acgacaacac
     5341 gcgattttag ccgagttgtt actgacgaca gagccgttga gtcttgctca tttcatggaa
     5401 tcatatcgca tcagtaatac cactttttat gcagatatca aacagttgga agaaagtatt
     5461 cgccaactgc ctttggaaat cattcggaac cagggatacg agatcgaagg accggaaaaa
     5521 taccgccggc ttctgacagc gaatgtatta gaattagaaa tcaatgaata tgaacttttt
     5581 catagtattt ctttcgatgc atcactgaat tatttttttc gattcgttga tccgcaacat
     5641 ctatctcttg cacgcaaagt aatcggggaa gaattgatcc aaaaaaagac caatctaagt
     5701 gatagaaagc ttgaacattt ggttttgatg ctgacattga cgatggagcg tgtgactaaa
     5761 aatcatttac tgacaaatga gacatataat agtctggcaa acaaagaatt actacagaca
     5821 gctaaacgat tgttttcacg gatcgcttct gagacaaaac aactttatcc agtaaatgag
     5881 atcgtctttt ttgcaggatt attaaatgac ttcacgaact catttgatca gaatttcttt
     5941 gaagagacgt tcgatacaca gcttgcttat ttagtaaaac aactgattca agaagtaagt
     6001 gaagagacga atgtccattt ttatgaagat gaaactttgt acaaaatgct gttgactcat
     6061 ttatctggtg tgttttcaag agccgtttta caagaagaga cactgaccaa tccgatttta
     6121 gaaaaaatca tggttcaata cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg
     6181 atttttcaag aaaaaaaatt atccgaagaa gagatcgctt atatggtcct tcattttgcg
     6241 aattcattgg aacgaagtcc gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt
     6301 ggattagctt ctactagcat gttggaacta cgattgaaaa ggtattttcc cttcatccaa
     6361 caaattcatt tctatcggat tgctgattta aaaaaaatcg atttacaaga agaatatgat
     6421 gttgttattt ctacatctat tttaccagga tacggtggaa aatatatttt agtctccccg
     6481 ctactgctag aagatgaagt caagcagcta aaagaagctt tttcgcatat tgagaagaaa
     6541 aaaaggcaaa aaattacgta taacagcaat aacgagtgga tgatagaaga tacttatgag
     6601 gaaacaatgc atttcatgga tcaaatcaat caattactcg gtcgtttttt tatccaagaa
     6661 ctggataatc ctgagacgat ttccgaaacg attcgcagct tatttcctta ttttcctgaa
     6721 ggactagtaa cggatgaaga ggtggttcat cgccggctgc ttaagcgata tgaacaagca
     6781 cctatcggta ttcctcatac aaatatgggg ttattccata catccagctc attgatttct
     6841 aaaccgatct tttgtatttt caatttgaaa catccgttga ccattgaagg gatggataga
     6901 cagccagtcg atttgtcccg tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa
     6961 gacgggattc ttttaggaaa gatcagtgga gcaatcatca tgaatgactt gaacaccgag
     7021 atcttccgct caggaaataa agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa
     7081 gaaataaaga aatgaaggtg aaaagatgat acagctacca gcagaaaaaa tattgctcga
     7141 tcaatcctat caaaattgga aagaagcgtt gaccgagatc ggccagtgga tgacccaaca
     7201 agggggaatc aaaccggcat atatccaatc aatgatcgaa agacaagaaa aagcgagtgt
     7261 ctatatcggg aatttcgtag ccttgccaca tgggactgac aaagatgaag aagtagtaga
     7321 agaaggaatc tatttagtcc aagtgcctga tggtgtggac tttggaacaa aagaggaacc
     7381 aaaaatcgcg acactcttat ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct
     7441 ccaagattta gcctttttct gttcagacat cgaccaagta atggcgctca gtgatgcgaa
     7501 aaccattgaa gaagtccaaa tgatttttga acatggacat atataaccaa tgttcacagt
     7561 aaaaaaagct gccttgcatc aagtaataac ttcaaccaag ataaaaataa gaggagctat
     7621 ttttgttggg aggttcttat tactgcggat gcaagctttt gaaaactatt tattctagaa
     7681 aaccgtttat ttgaaatagg ggacggtgac aaggtttttg tcaccgcccc tattattttt
     7741 gtgttttaat agctcttttt tatatagata gtgcccatac ctatgaataa taaaacgata
     7801 ctccatatac caagtgtcaa gataccattc atgaaagtaa cctgttctga agctagtggc
     7861 gagaaaatat ctcctcctat gatcacattt tgaaaatcaa cataagaagt tggcaagtaa
     7921 tgactgattg attctatcca tccaatcgaa gaaaacaagc gatactctgc taagaaaaga
     7981 caaagaagtg tacctaaaag attgactaga gaatgttcaa ataaagcaga tagaagaaaa
     8041 ctgattgtac ttaaaaagag cacccacaac aacaggaaca gaaagctctg gaaaagatac
     8101 tcatacatcg gaaggattcg tatctcctga ccaatgatcg ttcctacagg ataacggaag
     8161 tctcccaaac catttttgat tattactagt atagagacga tcaaaaatgg catcacgaga
     8221 cttagcatag ctgcaccata ataaatggta aaacggatgc tatgtttctt taccatacta
     8281 tccggtaata gattgactag agagattacc tgctttcttt tttcataaga gatgatggaa
     8341 gaaagaaaca aggcggttat acataaaatc aaaaaactag aaatcattcc tgaaaaaata
     8401 ttttcgtagt aatttgctaa agggagatgc gctggcaagt caaaaataga atatctttgg
     8461 atctgatgtt ctttcatata acggagcaac tcgacacgtt tttgttgttc aatgattgga
     8521 attccttgtc tttgaccaga aatcagtcga tccagtatat ctttttcata atgataagtt
     8581 gcttcgacca tttgctggac attccctgtg ttttttgcat gtaagattgc ttcgatcagt
     8641 ttgttgcttt tctctaaact ttccacaaat tctttctcat tctcatcttc tttcaacgtt
     8701 tcaatcaaac gttgcatctc ttcatagtta tgattcagtt catcgaattc tgtataagca
     8761 agtttatgat caaaaaaaag tgggaatgca aagagcacga ccaacaatag aggaaataaa
     8821 aagtttactt tgttttttag taagagattg cgttcaaatg taagtaaaga catagttaca
     8881 cctcatataa tttacgataa atagctaaaa tctcttccat atttgaggat tctgtgacga
     8941 tttctccatt ttttagaaaa catgctttag aacaaatcga ttggatattc gcgatagcgt
     9001 gggaagaaag aagaatcgtc ttcccctctt tctgccattg tctgatgagg ctattcaaga
     9061 tatcaatact tcctgggtct aatccgttca acggttcatc gaataacatg acagatgaat
     9121 cactgatcaa gtaagcagca agcaagacat gttgtttcat tcctagagac atttttccaa
     9181 tcggcaaagt aaggtactca gtcatcttca atcgttggaa aacagtttgg cattctactt
     9241 tgctctgcca atagtatttg atcaaagtca aataatcaac agccgataga tagtcggata
     9301 actgattcgt tgattctaga aaaaagaaag accggcgata atctgtataa gcagtaggag
     9361 agatgttgtg aagggacaat tgatcataag ttgttggcag cagttgggca atgccattca
     9421 gcaaagttgt tttgccaaat ccgttcgggg caatcaaagc agctacttct cctgattcta
     9481 ctaaaagatt caattgtcga atcacacgtt ttttaccacg tttcatgttc atgtttttga
     9541 tcagtaatgt catagatagg cctccaatat aaaatttaat aacataatag aatggaaaac
     9601 atctaaatta aatgctaaca aaagttattg tttgcatatt ataaaaaaat taaaataaca
     9661 gttttattta aaattgaagt tttctgatac cgaaatcttt ttattttgct gacatactca
     9721 aacctttggc acaaagtttc gtgccaaagt acgactattt ctctttttag taagcggata
     9781 caattagagt atcaaaagga gagagggaga gacaaaatgg aacggacgat tcccaaaaac
     9841 aacatttcgc taaaagcgag ggtacaaaaa ttaggcagta ctttatcaag catggtcatg
     9901 ccaaatattg gagcactgat tgcctggggt gtattaacgg ctctatttat cccagatggt
     9961 tatttaccaa atgaggcttt ggctaccatg gtaagcccaa tgctaactta tctgattcct
    10021 ttattgatcg gttatactgg cggaaaagta attgctggtg accgcggatc agttgttggg
    10081 gcaattgcaa cgatgggtgt gattgtcgga acagatattc caatgatgct aggcgcgatg
    10141 atcatgggac ctcttggtgg atatgccatc aagaaatttg atcagctatt ccaaaaacgg
    10201 atcaaatccg gttttgaaat gctagtcaat aatttttcag caggtcttat cggttttgga
    10261 ttagcgttat taggtttttc tgcgattggt ccagttgtcg atgcattgac tcaagcaatg
    10321 gcaaaaggtg tagagatcat tttatctgcc catttgattc cattgacaag tatttttatt
    10381 gaaccagcaa aaatcttatt tttgaataat gcaatcaatc atgggatttt gacaccattg
    10441 ggaactgaac aagtagcaga ggctggaaaa tctattctat tcttgcttga agcaaatcct
    10501 ggtccaggac ttggcgtatt acttgctttt actttgtttg gcaaaggagc agcaaaatct
    10561 tcagcaccag gagccatgat cattcacttt ttaggcggga tccatgaaat ctattttcct
    10621 tacgttatga tgaaaccgtt attattctta tcagttatag gcggaggaat gtcaggaagt
    10681 ttcattttcc aattgatgga tgctggacta agagcgccag cttctccagg gtcgatcatt
    10741 gcgattctag cgatgacacc gttcaattct taccttcctg ttattttggg cgtgattgtt
    10801 gccgcagctg tttcgtttgg tatttctgca gtaattttga aagcggatgc gaaagataca
    10861 aatgaagact tcgaaaaatc tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt
    10921 gcccaaggta ttcctcaagg agctacattc tctggcgtac aaaaaatcat ttttgcttgt
    10981 gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa
    11041 gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc aactgacaga tgactcgaat
    11101 acattgatcg ttactcaggt cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg
    11161 cgttttgtct cagtagaaaa ctttttgaat tcacctcgat atgatgaaat cattcaagaa
    11221 ttaagcagca agcagaaaac atcagtttct gaaattgaga atgagccagc agaagagttt
    11281 ctttcagcaa acatcaaaca aatcaaagag attctacttg tccacgatga tcgttccgga
    11341 tcagcaacaa tggggatgac cgtgctcaaa gatatattag acaagaacca tttaactatc
    11401 ccgttaagaa aagtcaatat ccaagattta aaagatgaac ctgattcact gatcgtagcc
    11461 aaagaaagtt tagcggaaaa agtacagatg gctgcacctc aatccattca tttatcagtc
    11521 aacagcatgg tcaatccgca aaaatacgaa tcgattgcac aatatttgaa acagacagct
    11581 taagggagga aaacagcatg caaagtttgc cattcgacat gatcgagctc aatcgatctt
    11641 ttaaaaccaa agaagaagcg atccgctatt gcggtcagat gcttgtagaa gcagggtgcg
    11701 ttgatgagga atatatcgat gccatggtag atagagacaa aatgttatct gtttatatgg
    11761 ggaattttat cgcgattcct catggaacag atgaagcaaa aaaatatgtg aaaaaatccg
    11821 gcatctgtgt cgttcaagta ccagatggtg tagattttgg taccgaagaa gaagaaaaga
    11881 ttgcgactgt tttgttcggt attgcaggag tcggagatga gcatttgcaa cttgtacagc
    11941 aaatcgcact ttattgcagt gatatggaca atgttgtcca gctagcagac gcgctcagca
    12001 aagaagaagt aacggaaaat ctagcaattg cttaaggaga gagaaaaatg agagcagtac
    12061 attttggtgc aggaaatatc ggtcgaggat ttattggaga ggttttggca gctaatggat
    12121 ttgagattac tttcgtagat gtgaatcaca cgatcattga tgctttaaat gaaagaaaag
    12181 aatacgaaat cgaattagca gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag
    12241 gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga aatcgctgaa gcagatttag
    12301 ttactaccgc cattggcccg aatatcttac cttttattgc tgagttgatt gcaaaaggta
    12361 tccaagaaag agaaaaggaa ggaagcacaa catctttaga tatcattgcc tgtgaaaata
    12421 tgattggcgg ttcagcattt ctagaaaaag aagtgtataa atatcttccg gaaacatcat
    12481 ttactgacaa atatatcggc tttccaaatg cagcagtcga tcgaatcgtc ccattacaac
    12541 accatgaaga tcctttattc gtccaagtgg aacctttcaa agaatgggtc attgacgata
    12601 gccaaagaaa aaataaaaaa atccaattaa aaggggtttt atatgtagat gatctagaac
    12661 catatatcga acgcaaacta tttagtgtca atactggtca tgcgactgtc gcttatacag
    12721 gtgccttgtt aggttaccag accattgatg aagcaatgca agatgcgtta gttgtggcac
    12781 aattaaaatc agttttgcag gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg
    12841 cagagcagca tcatgcgtat attgaaaaaa tcattcatcg cttccaaaac aaaaatatct
    12901 ctgatgcgat cacacgtgtc gctcggacac cattacgtaa gttaggttat caagaaagat
    12961 ttacacgtcc agtgagagaa ctacaagaac ataacttgac ttgcccacat ttgacggcaa
    13021 caatggggat tatttttaat tactatgatc ctgaagatga acaaagccgt caattgcatg
    13081 aaatgaagat ccatgaaaac ttggaacaat tgattcaaga agtcacagga atcaatgatc
    13141 cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa
    13201 ataggatacg aaaaagggga atgtgacgaa attttt
//
LOCUS       DAZUJO010000074        13227 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000074 DAZUJO010000000
VERSION     DAZUJO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.075"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8OL_002219"
     CDS             complement(<1..430)
                     /locus_tag="ACG8OL_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818190.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLS"
     gene            complement(441..1109)
                     /locus_tag="ACG8OL_002220"
     CDS             complement(441..1109)
                     /locus_tag="ACG8OL_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS4818191.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1240..1824)
                     /locus_tag="ACG8OL_002221"
     CDS             complement(1240..1824)
                     /locus_tag="ACG8OL_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4818192.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2159..2389)
                     /locus_tag="ACG8OL_002222"
     CDS             complement(2159..2389)
                     /locus_tag="ACG8OL_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS4818193.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2404..3354)
                     /locus_tag="ACG8OL_002223"
     CDS             complement(2404..3354)
                     /locus_tag="ACG8OL_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS4818194.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3351..4193)
                     /locus_tag="ACG8OL_002224"
     CDS             complement(3351..4193)
                     /locus_tag="ACG8OL_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS4818195.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4326..5051)
                     /locus_tag="ACG8OL_002225"
     CDS             complement(4326..5051)
                     /locus_tag="ACG8OL_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS4818196.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5512..5826)
                     /locus_tag="ACG8OL_002226"
     CDS             complement(5512..5826)
                     /locus_tag="ACG8OL_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS4818197.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(5842..7437)
                     /locus_tag="ACG8OL_002227"
     CDS             complement(5842..7437)
                     /locus_tag="ACG8OL_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFS4818198.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7460..8350)
                     /gene="iolE"
                     /locus_tag="ACG8OL_002228"
     CDS             complement(7460..8350)
                     /gene="iolE"
                     /locus_tag="ACG8OL_002228"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFS4818199.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8367..9392)
                     /gene="iolG"
                     /locus_tag="ACG8OL_002229"
     CDS             complement(8367..9392)
                     /gene="iolG"
                     /locus_tag="ACG8OL_002229"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFS4818200.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9416..10423)
                     /locus_tag="ACG8OL_002230"
     CDS             complement(9416..10423)
                     /locus_tag="ACG8OL_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4818201.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10536..11696)
                     /locus_tag="ACG8OL_002231"
     CDS             complement(10536..11696)
                     /locus_tag="ACG8OL_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFS4818202.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(11772..13226)
                     /locus_tag="ACG8OL_002232"
     CDS             complement(11772..13226)
                     /locus_tag="ACG8OL_002232"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS4818203.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         3950 a         2865 c         2174 g         4238 t
ORIGIN      
        1 ttgatagcga aaaaatgttt ttatctaata tatttgaaag atctaataat tcagctaatt
       61 cattgattct tttatccata tgctcttttg taaatccaaa gttttccatc gaaaatacta
      121 actcactcgt tgtatttgtt gtaaagaatt gtcctctagg atcttgactt acgtagccaa
      181 tattttttga tatactttct tgtgttaagg attgtagcga gttattttca aataaaattt
      241 ccccaaccaa ttttccgtca taaagtcttg gaataagtcc attcattaac tttaatagtg
      301 tggatttccc actcccagaa gaaccagtaa tcaatgtaag actacctttt tttattgaaa
      361 aattaaggtc aacaagcgct tgttccttaa catccttata tgaaaaagaa acattgttaa
      421 attcaaacaa ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt aacggaaaac
      481 aggatgatat agtctaataa gctcatgttt gttttatagt aagaacttct tttcacagga
      541 gattctatcc cctttgttat tgcattagcg gtcatttctt cagcaatcat agttgcacgt
      601 ataagaacgg gaactaacag aaattccaaa tattgcagag gctgaaaaaa agacaagcga
      661 atgtttctca tttttaaaga ttctttaatc attcttattt cagaacgata agtcggaaag
      721 aatctgatca ctactgtaat acttaacatt atttttttcg gtaaatgtat tttttctaaa
      781 gaggataata attcattgct agtatagaca ctaaccaaca accatgagat cattacgacc
      841 ggcaacattc gaaatacaat atataaggat gtcccaaaaa atatagtaaa aaactctatc
      901 ttgatagcat caataatttt cattactaaa aacagaactg aataaatcaa gatgaaataa
      961 atacatgtct ttattttacg aaacatcaat actaaaactg tagcaataat gaaacacccc
     1021 acttgaaata tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat atttaaagta
     1081 atcaaggtta acggattgat ttttgacact ctttcacctc caaaaaataa gatcgcactg
     1141 ttatcttaag ctgataaaaa tgcaccgtta ttcagataac agtgcgatct tatcaaaaag
     1201 cataactgag aacaattctc aatgcaagct tttttttaat taactcagta attcaatcca
     1261 cccaggaaca gaaaattgat acaatcttct ggttaatagc atagtctttt cttttgaata
     1321 gctatgcttc aatatatcat agaatgtagc gtagaatgag taaatgtaga agtggatttc
     1381 ttcttcatta aagactaaat tttgattgat cattccgata gtctttaact tatctagaca
     1441 acagactgtc ttttttgtca caaatgatgt aattttttca acaatatctt gatatttggt
     1501 tccagctgat tggaataaaa ccaaatcgaa tatacgttta ttctcaaaaa tataattaat
     1561 aaatggatct aattcattga agttctctct ccaacttttc tctatatcta tattttctaa
     1621 gtttaagagg tatttggtaa actcttcatt atacatatta ataagcgcat taatatgcgg
     1681 ctgaataatt tcttcaaata acgcttcctt agaagtaaaa tgtttataaa aagcacccgt
     1741 agttacgcct gcggtcaagc acagttgcct tatcttagtt tgctgaaatc ctactttttc
     1801 aaaatggctt agtccacttt ctattaattt ttggtgagtt gtaatataat ctctggtttc
     1861 tatgggtact tcctcctaat aagatgaatt aactatatta tcttacacag gacttgaagt
     1921 ttcaatcgtt aacgtctatt actgaactca ttccttagag ctaaactaat gagaaagagc
     1981 aaaataactg atacaaaaag caatcctaca agaattaata gactcatcta aatcattcac
     2041 cttttctaaa ataatccgag aaagatttaa aaaaattata tccagctatt aggataagtc
     2101 ctatcataat cactccaata gccattttct tatcctccct aaaaataaag ccttttactt
     2161 aaaacctttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat tgaaatccta
     2221 tatctaaata gtcctcatat ctaagtttag tcagcatctc acttacagat gctggactaa
     2281 cacttagaac ttttgctaaa tcattatttt ttaatttctt ttttcttttt gataattcct
     2341 gaattgctag taaataaatt agtatagtag ttgttttaat ttgtaccata cctaccctcc
     2401 aaattatttc gaaaggcctt cagcaatttt atccaggttc catttcatca tcgcatagta
     2461 actatcacca tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg aatagatagg
     2521 aatattggta tcttttgata ctgttttcat tggtctatcg tccacactgc tttctacgaa
     2581 taaggaggga acttttgttg tgcgtaattt ttcaactaag tgttttattt gatctggtgt
     2641 tccttcttct tcagtgttga tttcccaaat gtaagcagaa ggcacattat acgctttcga
     2701 gaaatattta aagcatcctt cacttgtaac aatcattttt ttatcattcg gaattgaagc
     2761 aaatttagat ttagcttctt tgtctagaga atccaatttt tcaatatact tatctagatt
     2821 ttctttatag aattttttat tatcaggatc tttttccgct aactgttttt caatattttt
     2881 agcgtaaata ataccgtttt ctaaatttaa ccaagcatgg ggatcttcct tccctttctc
     2941 actctgaccc tctaagtaaa taacatctat gccatcactt gctgcaaaat agtctttgtt
     3001 ttcctctttg ttcgcatttt ttactaattt tgtaaaccaa gcatttcctc cagtttccaa
     3061 gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat cttcaggcaa
     3121 aggttcatat tcgtggggat ctttcccaat aggtacgata ctgtgtaaat caattttatc
     3181 ttttgctata ttttcagtaa tatctgctaa aatcgaatta gtaactacta cctttaattt
     3241 ttcgttcgaa gccactgtat ctttgtcttt actatttgtt gaacatgccc cgaatataaa
     3301 aagagcaatc aaaatcccca gacttcccag tactgctttc cattttctca tcataacttt
     3361 ctctccttct ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat tacaaataag
     3421 aatgccgctg ttaaaacaat acttgatcct gctgctatgt taaaactata accaatgaac
     3481 aaacctagca atgatgagca cgctccgact gtcgccgaaa taaatatcat agtttttaaa
     3541 ctgtttgcat ataaatatgc tgtagcagct ggtgtaatta acattgcaac aattaatata
     3601 gttcccacac tttgcatagc tgttactgct actaaagtta atagaatcat taatagataa
     3661 tgataaaaac tgacattcat ccccattgct tttgccatta atggatcaaa agaagtgatt
     3721 aataattctt taaaaaatag aacaactgta accaacacta aaacagaaac gccaattgta
     3781 atccacttat caatatcttg tacagctaaa atatttccaa ataagatatg aaataaatcc
     3841 gttgagctat ttgcaactcc tataaggata actcctaatg ctaaaaaaga actgaaagta
     3901 ataccaattg ctgtatctcc ttttattaca ctattgcttt ttataaaagt aataatgata
     3961 gaagctaata gaccaaatac aatcgcacca ataaaaaaat taatacctaa aatataagaa
     4021 attgctactc ctggcaaaac agcatgagaa atagcatcac ccataagaga cattcctctt
     4081 aaaataataa aacaacctac tgttccagaa acaatcccta taactacaga tgtaattaga
     4141 gcattctgta aaaagtgaaa gtcatacaga ccattaataa aattttgaag catattattc
     4201 cccttgtata ttagagttag ggttttaacc ttccggtttt gattggataa ccctatgaac
     4261 catcgttatt ttaatttggt attaactcat ttcttttgag ataatatatt tattggattg
     4321 agaggctaag ccgttccccc ctctccaata aatatagtat ctccataggc tttttttaaa
     4381 ttctcttcat taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga
     4441 ataatattat caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt
     4501 tttccctgat gtttcaattc ttttaacgta tccataatta ttcgttcact aactaagtct
     4561 atccctacaa atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct
     4621 aaaagtacac gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt
     4681 cccatatcga ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt
     4741 tgaaaaactt tcattccggc atatgttccc atagatacac attcttttac ttttattgga
     4801 aaagtaaaat caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg
     4861 atatcttcat tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta
     4921 agcaatgtag attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt
     4981 atttttaaag aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc
     5041 acttctaaca taactttctc cttctaaatg ataatgatta tcacgatcat atggtataca
     5101 aaaattatat tttagtcaac cctaaatttt ataatttaga tttttttctg ttgttgaaga
     5161 ttaggatgtc aaaattattt gaaactactc gtatagtaaa tagtaagtaa ttttcaaaac
     5221 acgaagaaaa acaaaaagaa aaaaggaaac gccgaagcat ttcctttcag tgatttaatg
     5281 agaacaacac gatttgactt agagtctcaa aaagtcgaat aggtacactc atttaagtgt
     5341 ctccattcga ctttttgggg atgagctggc tattgaggtc cgcttaccct attgaataaa
     5401 tacaagattg ggtatttcta ataagtccgc ccttcatatt ggggtgatcg gggcgaacat
     5461 gccatctttt catataccac ctcctcgaca ctcaataatc aaagattctc gttaatcttc
     5521 ttcgatgctt gcaatgatgt tttcaatatt catataacga aaatcttctt gtttgatata
     5581 aacgatctga gcggtacatt tttccttttt gatgatttct tttacttttt cctcagaacg
     5641 tgcgacctgc ggcgttaaaa gtaaaacgtc atagtcttta taattcagtt tttcttcaac
     5701 gtttcggatc gttgcataat ctatctcata cgatagattt ttggctctga tcgtttcaag
     5761 catttttgat acgagcagcg acgcactgat tccggtctcg caaataatca atagatcctt
     5821 atgcataata ccctcctgtt tttagcctgc aaagccaatt ggtgaaaatg ccacataagt
     5881 taaaataaca atgactacca atgctatagc agtcaccttg gtgtatttcc aaggctcgat
     5941 atcgaccttt gtatcgaatg agttcaacac aaattctcca tcaggtttca atttggaaac
     6001 caccatcaaa atgataatgt ccagaacgaa taatgccgtc aaaacataaa ggaaatggat
     6061 attttggaaa agcatttttg atagtgcata caagacgata tgcacggcaa tcgtcgtttt
     6121 tgccccaaaa gcagttgctt tttttgaata aaaggcaaag agaatgatca cgagcagcgg
     6181 catgttgtat agtccattga attcttgaac gaactgatac aatccagccg gtgcaaaaga
     6241 aatgaatggc gctacgataa tggagataat ccccacaacg accgttactg ttttaccagc
     6301 acgagcgatt tgcttatcat ttgctttctt attgataagc ggtttataaa aatccagtga
     6361 aaacaaggtc gatgtcgcgt tcaaagctcc cgcaaatgat gataagatcg cgccaaagat
     6421 aacagcggcg aaaataccaa agatcatttt aggcaatact tcactgacta aaaaaggata
     6481 cgcgttatct ggatttgaca ttaaactatc gccaaaaata ttgcgagcga cgatccctgg
     6541 aaagacaagg aataacgcac ctaagatttt gaagacgccg actaggaaag cgcccttttg
     6601 tgcctcagct aaattcttgc cggacaatgc tttttggacg atcatttgat tggtacacca
     6661 gaaatacaga ttgttaaaca gcatccctgt aaatagtgtc ggccatggaa caacagccga
     6721 gtcgatcgat cccacagagt taagcagcca tggcgtgttt accacaacgt gttcgatccc
     6781 gccaacaaag ctgccgtcac caagcttcat taagcctaaa attgggacca gcagaccaca
     6841 agccagcaga ccgactccat aaatggagtc actattggca cttaacgaca agccgccgat
     6901 cagcagatag aggatgccga caataccaat tgccatcgct acaattatga tcgcgacgat
     6961 cggctggacg cccagcagct catcaatatg gaagatctta ttgaacacca gcgatccaga
     7021 ataaagaacc acgggtaaga aagaagtcat ataggtcaca ataaataaga tcgaaataat
     7081 tcgtttggta accgtatcat aacgaatctc gatgaaatct gagattgtat tgatgccgta
     7141 tttaaaatat tttggcaaga atatcacagc caaagctacg atcgcaatcg tagcagttac
     7201 ttcccaggcc attacttcca tccctgcatg ataactttga ccattttgtc cgactatttg
     7261 ttcggtggac aggttcgtca tgatgatcgt gccggcgatt ggcaaagcag ttaagctccg
     7321 ccctcccatg aagaaccctt ctgatccaga agtatcgaca cttctggatc tagaataggc
     7381 aaataaccat acacagatga caatcactag gaaactgatt atagaaaaaa gattcatcgt
     7441 ctagctcctt ttcaatagat taaagaccgg tgacctctcg aatgtagctt ctggctttga
     7501 ctgcatattc aaatggattg gctttagccg ggtcctgttc tgcttcaact acgatccagc
     7561 cttcatagtc gctggcatgg attgctgtcc agattggagt gaaatcaatc atcccatcac
     7621 caggaaccgt gaagacgcct ttcttcacgc cgtttaagaa gctgtctttt tccttttcta
     7681 cttcttcagc cgtttcttga cgaatatctt tgaaatggat gtgtttgata cgatcttgat
     7741 gacggttgta aaccgcgatt ggattttcac cagaaaagac caaatgtccc gtatcgaaca
     7801 ataatttaac tttgtctgga tcagtcattt ccattaaacg atcgatttct tccgtcgttt
     7861 gaacgccggt acccatgtga tgatggtaaa cgatctccat atccttttcg tgggccaaat
     7921 cccctaattt ttctaatcct tccgccagtt ttttccactc ttcatcagtg aaaactggtt
     7981 tatcatcaaa gactggcgct gtcataatcc cttggatact atgaccctgc tcagctacta
     8041 cgatgacttt agcgcccatc gcatgtaaga aatcccgatg tgctttgaag gcttcggcag
     8101 tttcttcaaa aggtttagtt gtcagaaacg cactgaacca tgcactggca acctttaagc
     8161 cgcgaatatt caaatattgt tccaacacct ctggatcttt tggatatttg ttgccaatct
     8221 ctgttccggt gaacccagca agggccattt cactgataca ttgttcaaag gtattttcac
     8281 taccaagctc gggcatatca tcattggtcc acgcgattgg tgcgatggct aattgaattt
     8341 gtttggtcat attctcacac tcctgcttat ttttttagtt gtactaaact cttcgtatcg
     8401 aatgattctt gacaagccaa cgcgatctta gttgattgca atccatcttc tgcattaacc
     8461 gctggttgac gtttttcttg aatacatgag acaaattctt gggcttcacg aatgaaggct
     8521 tctctgaatc gttctgggaa gttttgtgaa catggacgga tgatcccttg atcgttcatg
     8581 atcgtcacaa gatttttctc aggcgcttgc gctacccgca gcattccttt ggttcccatg
     8641 atctctgttt cgacatgata gccatgggct gcgtttctgc cggcaactag taaagctatt
     8701 gttttatcgc tcaggctcat cattgcggct ccagtttcaa gctcaccagc ctgatccaat
     8761 tctggatacg ccgcattttt cccaatcgcc cacacttgat cgacttcttg gccggtaaac
     8821 cagcgaacca aatcaatatc atggatcgcc atatctaaga acaagccgcc gctgtcgtta
     8881 ttcatggcaa atttgacaaa gctttccaag ccttcgctcg gatcgatccc ataacaacga
     8941 atcagtgtga tttcgccgat ttcgccatct tctaccattt tcttcgcata ctgatacgac
     9001 tcatcatagc ggcgcatgaa gcctaacatg aagacctgtt caggatgctc ttcgatgact
     9061 ttttgggtct ttttaatatc ttcaacttca aggcctaatg gtttttcagt aaagacatgc
     9121 ttgccatttt ccatcgccaa gcggacttgg tcacagtgaa agcctgatgg tgaaacaata
     9181 aagactgcat cgatcacgga acttttgacc atttcttcat agctggaaaa agtatgcttg
     9241 acgcctaatt tcttctgtgc gaatgctaat tctgtttcaa ctaaactgca cgcagccgta
     9301 agctcacaac aactgacacc tgttgctaaa ttttgtgcat gtacttttcc taatcttcct
     9361 aaaccgacga tgcctactct aatgttgttc atgaaatacc tccgttttct gagaattact
     9421 cattgtatag tgctggtcgt tcatccattt gaatcattac ttttgttttt gtttcacgag
     9481 actcatgaca agccatcatg gtgatcgctg ctacataccc atcccaagcg gtaggaccga
     9541 caaccgcatc atttttgatc gaatcgatcc acagcttgaa ttcataatca taggcatcca
     9601 aaaagcgggt ctgccaatct ggtgaaacag gtgtgtaatt tttttcttcc gatttaattc
     9661 gtgtataggc tgggtccgtc attcctactt ctgccgtttc gaaaaccaca tcacatttaa
     9721 tatcgtagcc aaagcggcaa ttcacaaaga tttccaaatc aatcgttaca ccggattttg
     9781 tttgaaagat cagcagctgc ggatcatgca aatcggcatg ggagttgctg gtttttttcg
     9841 gcaagatcat ttgaacagat tcataatctt catttagcaa ccagcgaagc acatcaatct
     9901 catgaatcgc ggtgttgctg atcgccatcg gtgttgtgta gttttcatct gcgctaggat
     9961 ttctatgggc acaatgcatc attaatgctt ctccgtattg cttcgattcg attgcttctt
    10021 tcagttcaat gtagccgcgg tcatagcggc gcatgaagcc aacctgaacc aatttcttcc
    10081 cataagcaac ttccgcatca acgatccggc ggcagcctgc tgcatccatc gctaatggct
    10141 tttcgcagaa aacatatttc ccatgcttga ttgcttctaa cacatactgc tcatgggtcg
    10201 ggtcccaaga agtcacaaca acgacatcca catcttcaga agcgatcaat gcttctccgg
    10261 tttcaaagaa tttagcatcg acacgtttgg caacctcttt agcagctttt acattgatat
    10321 ctgagactgc tgtcacataa gccccttgtg ttttctgatt gatcctgtcg atatgctcga
    10381 tgccgattgc acctgttccg ataacgccta cttttactct caattactta tccccctgtt
    10441 ctgttgttta agcacacata gtatgcgctt acaaaattat tcctgtgtag cttttattct
    10501 taaaaggtag agccctctct ttgctccgcc ttttactacc cataagcttg tacgtataat
    10561 tccttaattt gactatgact aggcaccttt ttattatttt gcggacttcc gctggtcaac
    10621 gcatccgcgg ccattttgtc taaggctgcc ataaacgctt gctgatcaat accataatct
    10681 cgaatcgttg ggatcgacag ctgctcacag agctcttgga tttttttaat cagataatcc
    10741 gcagcctctt ccagcgataa atcttcggct gccggataaa taaatctggc gatcgtcgct
    10801 aactcagtct gaatagcatc ctttgagaat tcaaaaaaga ccggcaataa catcgcattt
    10861 gatatcccat gaggaatgtg aaaaagtgcc cccaccggtc gactcattcc atgaatcaat
    10921 gtgactgacg cattcgaaaa ggcaatcccc gcctccgttg aagctaagct catttgttct
    10981 ctggcttcag tgtcgtcgct attttcatat gcctgagaaa gcgtgttcaa aattttcttc
    11041 atcgcttcca gtgaaaatag ttgcgttact tcattggctt tgactgaaac ataagattcc
    11101 aaagcgtgac atagcgcatc gactcccgtt gccgcggtta cttttggtgg tacagttttc
    11161 gtaaaatcag agtcaacgat tgagatctgt ggcatgaaaa cgagatcctt catcatcatt
    11221 ttgactgctg tttttgtatt ggtaataact gccacatcgg ttacttccga accagttcca
    11281 gcagttgttg acactgcaat cagcggaatg cccgcttgca gctgatcaaa atttcttgca
    11341 tgaatcgctt cgagacttaa ttgattagca gccaaaacgg ctaccacctt ggctgtatcc
    11401 agacagctgc cgccaccaaa agcaatgata tgatcacatt gatgcttctg aaaaagcttc
    11461 agcgcatcat aaacattttc ttgcgtcggt tcggcttgaa tgtctgtata ctccgctaat
    11521 tgatcatctg tttttcccaa cagctccttg atcttttgaa gatgcccggt ctgttccata
    11581 accgtatcac taatgattaa cggcttgttg cctagttgtt ctgattcttc cataagaaat
    11641 tggattgttc cgccgccaat tcttaaattg gcaggcattt tcagttgaat attcacagaa
    11701 aaaacctctc tctggcttat agcctataac agaatcctcg aagctcttta acagagcttc
    11761 gaagcaaatc tttaaatctc actttgcgca taacgtgctg taacaacttt ccgatgggta
    11821 taaaactcaa cactgtcttt gccattggca tgcaaatcac caaagaaaga gtgcttccag
    11881 cctgaaaagg caaaccaagc catcggtgca ggcaccccaa gattgacccc cagcatccca
    11941 gcatcaacat tttctctgaa gtagcgaatc gctgatgcgt tgttggtata cagacacgaa
    12001 ccattcgcaa attcagattc attcgtcagc tcaacggctg ccatcaaatc ttttacccgc
    12061 acgattgatg cgaccggggc aaaaatctca tcctgccaga tcttcatatc catcgtgaca
    12121 ttatcaaaaa tcgttggtcc aacaaaatag cctttttctg gaatgttttc gcgaccatct
    12181 agtaccagtt tcgctccttc atcgacccct ttttggatat agtcaaaggt ccgctgctgt
    12241 gcatcttttc gaatcactgg tcctaaaaag accccttcgt ccaagccatt gccgatcttg
    12301 atcgcttttg ccgcagcctt aaatttttca acaaactgat ccgcgatttc ctcttccaaa
    12361 acaatgacgg aggctgccat gcagcgttca ccagcagaac caaaagctcc gccaataagg
    12421 tccttgactg cccgatcaat attggcatct ttcagcacaa caacatgatt tttcgctcca
    12481 gtcaacgctt gtacccgttt tagatttttt gttccttcac ggtaaacgta ttcaccgaca
    12541 ttttttgatc ctacaaagct gatgcctttt accagtggat cttgcagcaa tgtgttgaca
    12601 acatcgtggg caccatagac aacattaaaa acaccgctag gaaagcctgc tttttcaacc
    12661 agctcaacaa ttttctccat caacaatggt gttttttctg aaggcttcag caccaccgta
    12721 tttcctgccg ccagagccat cgggaagatc cagaagggca ccatcattgg aaaattaaac
    12781 ggtgtgatcc ctccgattac tccgattggg taacgataat tcgtgatctc aatatcagtc
    12841 gccgctgttg ataaagaatc tcccatcatt aaattggtga ttcccgctgc gtgctccaca
    12901 ttttcgattc ctcgttgaac ttctcccagt gactcggaca atgatttccc attttccagc
    12961 gtgatcaaac gcgccaattc acttttatga tctgttaaaa gctgatgcag cttgaacaga
    13021 taacgtccgc gaacaggaac aggaactttt ttccaagttt gaaacgcagc atcggcagct
    13081 ttaactgctt cggctgtctc tgctttgcta gacaacggta cattcgccag cgcttcttct
    13141 gtagccggat tcattacttc ttcaaactct gttgagcgac tagcgaccca acgaccatca
    13201 atataatttc ttaaaaatct tgtcatt
//
LOCUS       DAZUJO010000075        13085 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000075 DAZUJO010000000
VERSION     DAZUJO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.076"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG8OL_002233"
     CDS             complement(79..2199)
                     /locus_tag="ACG8OL_002233"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4818204.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG8OL_002234"
     CDS             2429..3607
                     /locus_tag="ACG8OL_002234"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS4818205.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8OL_002235"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8OL_002235"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS4818206.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8OL_002236"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8OL_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS4818207.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG8OL_002237"
     CDS             complement(4960..6213)
                     /locus_tag="ACG8OL_002237"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4818208.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8OL_002238"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8OL_002238"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS4818209.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG8OL_002239"
     CDS             7790..9136
                     /locus_tag="ACG8OL_002239"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS4818210.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG8OL_002240"
     CDS             complement(9444..9830)
                     /locus_tag="ACG8OL_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4818211.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG8OL_002241"
     CDS             complement(10098..10247)
                     /locus_tag="ACG8OL_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818212.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACG8OL_002242"
     CDS             10709..10921
                     /locus_tag="ACG8OL_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818213.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACG8OL_002243"
     CDS             complement(11199..12998)
                     /locus_tag="ACG8OL_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS4818214.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DAZUJO010000076        12803 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000076 DAZUJO010000000
VERSION     DAZUJO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.077"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACG8OL_002244"
     CDS             5..2377
                     /locus_tag="ACG8OL_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS4818215.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMVEHQRKTNGQ
                     PMNDKRNGRSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKKMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNESVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ELSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYHEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACG8OL_002245"
     CDS             2438..3898
                     /locus_tag="ACG8OL_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS4818216.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAELFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     FHFETEKYKDHPVDSQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKDI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACG8OL_002246"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACG8OL_002246"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4818217.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSADMYDENWKQWSLEHLPIFPNQYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGEMIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPESFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKSQITAQNGIFDAKFDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPVDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPKPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNRRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACG8OL_002247"
     CDS             6129..6278
                     /locus_tag="ACG8OL_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818218.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6359..6955
                     /locus_tag="ACG8OL_002248"
     CDS             6359..6955
                     /locus_tag="ACG8OL_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS4818219.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMELDRKNLRKLNEYRRILPTDKTMRSYIKALN"
     gene            6968..7867
                     /locus_tag="ACG8OL_002249"
     CDS             6968..7867
                     /locus_tag="ACG8OL_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS4818220.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQINREMER"
     gene            7870..8355
                     /gene="ssb"
                     /locus_tag="ACG8OL_002250"
     CDS             7870..8355
                     /gene="ssb"
                     /locus_tag="ACG8OL_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS4818221.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKSATESRAHADQSSTSPNTTTFEQHDTMTTNNNGLNASQNPFGGQSIDISDDELP
                     F"
     gene            8377..8634
                     /locus_tag="ACG8OL_002251"
     CDS             8377..8634
                     /locus_tag="ACG8OL_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818222.1"
                     /translation="MAKNISLTMDLLIPVIAFIDGISLGITENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(8793..8915)
                     /locus_tag="ACG8OL_002252"
     CDS             complement(8793..8915)
                     /locus_tag="ACG8OL_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS4818223.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9069..9329
                     /locus_tag="ACG8OL_002253"
     CDS             9069..9329
                     /locus_tag="ACG8OL_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818224.1"
                     /translation="MEHYTGLLDKIRVIKTHPLLVRFTLRTNHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            9777..9983
                     /locus_tag="ACG8OL_002254"
     CDS             9777..9983
                     /locus_tag="ACG8OL_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS4818225.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9983..10234
                     /locus_tag="ACG8OL_002255"
     CDS             9983..10234
                     /locus_tag="ACG8OL_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818226.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            10250..10687
                     /locus_tag="ACG8OL_002256"
     CDS             10250..10687
                     /locus_tag="ACG8OL_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818227.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            10680..11375
                     /locus_tag="ACG8OL_002257"
     CDS             10680..11375
                     /locus_tag="ACG8OL_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818228.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            11540..11740
                     /locus_tag="ACG8OL_002258"
     CDS             11540..11740
                     /locus_tag="ACG8OL_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818229.1"
                     /translation="MNKIIPLLLVLFVGEILMRLSAAVHLNLAVFFVILMFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            11756..11935
                     /locus_tag="ACG8OL_002259"
                     /pseudo
     CDS             11756..11935
                     /locus_tag="ACG8OL_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11997..12181
                     /locus_tag="ACG8OL_002260"
                     /pseudo
     CDS             11997..12181
                     /locus_tag="ACG8OL_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            12215..12646
                     /locus_tag="ACG8OL_002261"
     CDS             12215..12646
                     /locus_tag="ACG8OL_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS4818230.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         4707 a         2154 c         2701 g         3241 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgaa aagatggttg aacaccaaag
      121 aaagaccaac ggtcaaccaa tgaacgataa gagaaatggg cgttcttggt ttaggcaaca
      181 agagcgaatc gaaaataaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 agtagaaaaa cttgaaagac aagaagagct aaagaaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgaga atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 atatcaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctctgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt agatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg taaaaaatga atctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaactatcct ttagaaacca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagagg ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtaatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaacagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattgggat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcatatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttggcta caagtattca
     1741 cgaattagcg catgctagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaact
     1801 acaggcagaa atgacctcct atattgtgtg caagcatttt gggatggaca cgtctgagaa
     1861 ggcgattcct tatattgcga catggacgaa gaatggtcag actttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccagc cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 tcatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctga
     2641 actttttttc atagacaaag agattcaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aattttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattctca accactcaca gaaaaaggaa ggaattattt
     2821 gaaggaagaa cgaaaactgc ccgattggtt gattgattat gcagaaaaag aaggattgat
     2881 cgccgaacta aagccaaaac atgaacggca aaatttctta gttcgagacg atcgtttgga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaaca ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggatttaa cctaaagatc ggtgacccta aacagttaaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagttga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaagacattt atgaagaagt tgcaaaagaa atgaaagttg aacctgaagc
     3481 aattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaat tggtttccgc
     3541 tcacaaagta aatgcgtctt ttggtcagca gctttctgtt aacgattcaa tagaagctat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagcttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg ctcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggtaga cagcaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccacc cgaaaagacg ggtattttga gatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgagttagat tcggccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagagcattt gcctattttc cccaatcaat
     4141 accactatca tgttcctaaa gataagaaaa agcaattcaa tgtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa atgatcgctt
     4261 ggacgatcat tcaacaagca ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcaag gattccagca gttaagagac gcaggagaga
     4381 cctatcccaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta atcggtatga
     4441 atggcagccc gctgtatagt ttactcttgc agcaaaaagg cataccagaa agtttttcct
     4501 taggtagagt ccaaactcca acgctttaca tgatttatca gttgcaggaa aagattagaa
     4561 acttccaaaa agaaccatat tttgaaggga aatcccaaat aaccgctcaa aatggcatat
     4621 ttgatgcgaa gtttgatccg aatgaaaccc aagcgacgca agaagcattt gaagcttatt
     4681 taaaagaaaa aggtgttcaa gtaggaaagc agccaggcac gattcttgaa gtcgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagcttat gaaagctagt gccaaagaca cattggaagc tatgcaaggc ttatatgaag
     4861 ggaaatatct aagctatccg cgaacagaca ccccttatat tacggaaggg gaatatgcgt
     4921 atctgttgga tcatttagat gaatacaagc attttttaaa ggccgaatca atcccaaccc
     4981 ctattcatac gccgaatagt cggtatgtga ataacaagaa agtccaggaa cattacgcga
     5041 tcattcctac aaaaacggtt atgacagcag cggcttttga acaactaagt ccacttcagc
     5101 aggccattta tgaacaagtg ttaaaaacaa cggtggccat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaggttc agcagcttca gttaaaagca atcggaaaag
     5221 tgccggtaga tctaggttgg aagaagttat ttggaaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccaaaac cttataccga aggtacgttg atcactgcaa
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagctcgtgg tgacgaataa agggattttg ctttgccaag
     5581 ccgtggaaaa agagccgctg cttacgagcg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggggaacga aaaggaacac aaaccacttt tctagccaat atccaaaaat
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccactgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagca tgtacgtctg
     5881 aagcctgtga ttttaagtta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agacgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaga gaacacgacg cactacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa attgaattag atcgataaaa tcttcctggc tattgtatac
     6301 gtataaactt atgttatact atatttataa gtttatacgt atacaaagga gggagaaaat
     6361 gacaaaagca atggtgtaca agctgctaga agaatttaat ggaactttgg ctttgaagga
     6421 tgccaaaaaa gcgggcattt ctcctgtaac gataaaacgt atggtagatc gtggagagct
     6481 ggatcgtgag tatccaggtt ttttcacctt acctggtcaa tttcctgatg aattgtttat
     6541 ggcgcaaaaa aaatatgaac gcggcattat ttcacatatt accgctttag atttgtatga
     6601 tttgacagat atgatcccta gacagatcga tctgactgta ccttacggtt atcacgtttc
     6661 tgaaaaagga ttgaaggaat tcgcagttga gctacactat acaaaagcgg aatggtatga
     6721 attaggtaag attgaaataa aaagtcgcta tgggaatcct attatagctt atgatccaga
     6781 aagaactttg tgtgacattt ggaatccctg gtataccgtt gaggatgaga tcaaagtaaa
     6841 agcaatcaaa aactacatgg agttggacag aaaaaatctt agaaaattga atgagtatcg
     6901 acgaattttg ccaacagaca aaacgatgcg ttcttatatt aaggctttaa attaaaggag
     6961 aagatacatg acacctacgc agttaaaagc aaaagtgaag aatatttcca gagaaaaaga
     7021 agttgatccg cagcttgttt taagacattt tatgatggaa aagttcttgg aaaaaatctc
     7081 agagtctcct tatcgtgaaa attttgtgtt aaaaggcggc ttcttaattg gctcaaagta
     7141 tggcattgaa aatcgcacaa ccaaagacat tgatacgact ttacgggaaa tgaaagtaac
     7201 caaagaaaca ttaacaacgg ttctaaacga tattttttca acgccgacga aagaaggcat
     7261 tcaatttgag attcaaggaa tgaaagaaac gagagaagcc gattactatc caggatttag
     7321 cttacgtgtg ctggcacatt tggaaaacat gcggccagat tttaaggtag atgtgactac
     7381 aggtgactcg atctatcctg caacaatcac tcacagtcat aaattgatgt ttgaggatcg
     7441 aacgattcct ttggaatcct atcctacaga acaaattatt gctgaaaaat taagtgcaac
     7501 ctttgatttt ttaacagata attcaagagg aaaagatttt tatgatctct atactattcc
     7561 taaaatggag aagatcgatg agaagacctt gaaagatagt gtgagaaata catttacacg
     7621 tcgaggaaaa actgatccgt taaagattta ctatgaaaag gatatggatg tactgaaaaa
     7681 tagtcacgag ctaaaagata cttggaagaa atatcaaaaa agcaatcctt atgctgcgtc
     7741 gatcacgtac gatgagacaa tggattcgat tagtaatttg atggaaaaag tgattcacgt
     7801 cgaagaaaaa gagcgaaaac tagcttatca aagacaccaa cagataaata gagaaatgga
     7861 gagataaaga tgattaataa tgtgacatta gtaggacgct taaccaaaga tcccgattta
     7921 cgttatactg caagtggcac agcagtagcg acttttaccc tagccgtcaa ccgcaatttc
     7981 acgaatcaaa acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca
     8041 gcagaaacga tggcgacatt agctaaaaaa ggcagtttga tcggcgttgt cggacggatt
     8101 caaacccgaa cttatgacaa ccagcaagga caacgggtgt atgtgacaga agttgtggcc
     8161 gataatttcc aattattaga aagtaaatca gcgacagaaa gccgtgcgca cgctgatcaa
     8221 tcgtctacca gtccaaacac caccaccttt gaacagcatg acacaatgac gactaacaac
     8281 aatggattga acgcttcaca gaatccattt ggtggacaat caatcgatat tagcgacgat
     8341 gagctaccgt tttagttcga gaaagaagga gtaagcatgg caaaaaacat tagtttgacg
     8401 atggatctat tgatacccgt tattgcattt atagacggga tttctttggg tatcactgaa
     8461 aatcctttgg taaatagaat tgcgaaagga tcactatttc ttgtattcgt gtatcttgtc
     8521 tgttcttatc ttttgaaaag agcaggaaag gataattcat tttggcgatt gactaaagtc
     8581 acaatagcag cgacagctgt tatacttacg gctattctac cgctttatat gtagggttaa
     8641 cgggaaatcg ttgaacttct tgcgaaatat tgctataatg aacatgaaaa agaggagttg
     8701 cgcgaacaac ccctcattaa cagcaccgat taaagaacgg tggcagaata ttagttattt
     8761 ggaaataacc gctgacctgc taaagtagac ggttatttct ttttgtcatt tttaagcata
     8821 tttacaatca aactaatcaa agcaatggta aacataccaa aaccaagaat ggtttgaatt
     8881 gtctcatatg cggacaaaag gcttactcct ttcatagatt ttagcactta cgttcataag
     8941 caccaccacc tttcagaagt cgccaccgtc ttcaaacttt actgtcttga tcattatagc
     9001 atatctaaaa tcgattaaaa attctcaatt ttagctgttt ttgctataat tttagcgagg
     9061 tgatcgcgat ggaacattat actggtttac tggataaaat tcgggtaatc aagacacatc
     9121 cgttgcttgt gcgatttaca ttacgtacaa atcatgaacc gattaattgt gtggtggcca
     9181 atatagaaat agcggataaa ttgctgattt tggaagaagg caagtatact atttcagtga
     9241 caggtcactt caataaacga aatcaactag tcattgaatc aatgcaaatt agaaaccctg
     9301 atcattttac gagattaatg ggcatataaa taaaagaaga gctgcagtcc taaatgagga
     9361 ttgcagctct ttttgtttta catagaaaca agaaaaatga aaaggccttt ttaggaaggc
     9421 gcaagcgcct tctcaattga aaaacctctc tctcactcca accgccaccg ccgttcgaga
     9481 agaaaggaag tgaacgttgc acgttcacta agataagggg tcttgcaaag caagacaaaa
     9541 tagaaaaaac ttcctctcac caaaaggttc gttcctgttc gctatctctt tattgatttg
     9601 ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag ggtaaatggc
     9661 acgctgcacg cccttgtcct tgtcgctttt agttgattca ttctggccaa caaatcaata
     9721 aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa tcaatcatgc
     9781 gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac tatcatgcac
     9841 catatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc tattgtttgt
     9901 atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattat attttgattg
     9961 atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgactg taaaagaagt
    10021 cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa gtagcagaag ctttacttat
    10081 tccgcggtac tggacgatag aaaccccttt agtagaggat gcaccgacta tgctatttga
    10141 agacctagtg aagattgcag gatatgaact gcattatcaa gaaaacatgg aaacttatga
    10201 aaacgaatat tggttaacca aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa
    10261 ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa tggaggatat
    10321 gacattccta tacaatgaga cacgtgttcc aaaaaaacat tataaggaaa gattgtcggt
    10381 agctgtggaa gaaatgattg aatcgggtgt tgaaatgaat ctcattgcga cctactatcg
    10441 aacgcttgaa gaactgaaaa agcaaaatgg acaatggttt ttccaagcct tactatgctt
    10501 ggaagcaggc gtcaagccaa acactatcaa accttctgaa taccaagctt tagaactaac
    10561 gtatgggaaa tttgtcgaaa cgaaaaaagc caagactgtt tcaagtgaat ggttggacta
    10621 cttagaaaat gtcaacaagt atggcgcatg ttacatcatg aaaaaggagg acaacgaaaa
    10681 tgaataaggt gatttatcaa ctaacaacgg aagatgtgtc acacttttta acaaacaaag
    10741 aacatttgaa tgaagtaatc agacggattc aaaaagactt tagttgtatg gaagaaatga
    10801 agtactttat tcaaagagtc gaagaacaat tgtatttgga aagtgaaacg agtcaaacga
    10861 aggttacagt cagcaataat tatttagacg tgtataaacg tcgtcctgac acattaaaaa
    10921 aaggagcgga gcttctacga ctagaaacag aaaaatatat catgaatgtt tacctagaaa
    10981 ggagtgaact ggtcgaaaac tttgtcagct acacgatttg caggaaacac tattactcag
    11041 aatcagaata ttattcaatt tttgagagca tggaattaat ggaagagcta aacagcaaag
    11101 atgcgacggt agacgattat taccttgtat ttactaaatt tttgacgtgt gggacggaat
    11161 ctatcgtgtt aatgggaatg gattatgaac tcgctgtacc cgaagtattt ttgagggaat
    11221 atgtagaaga agtagataac ccaggcgacg actggtacac gactttaact gattttttag
    11281 atacctacac ttacgacgat acagaagaaa tccaaaatga acttgaccgt aaacgaatgc
    11341 catatcaacg atttgaaagg aaaaaaggtc aataaccatt tataccgaaa agtgagaatg
    11401 tgtgaaataa gaacaaaaaa ccttgaaatt aagggaatgg ttcttatttc gcaccaatcg
    11461 ctccaaccgc ccccgccgct taggtggtgc gaaataagcg agcaaagccc gaaaacctaa
    11521 gagaaagaag gagatttata tgaacaaaat tattccctta ttattagtcc tatttgttgg
    11581 agaaatactt atgcgactta gtgcagctgt acatttaaac ctcgcagtat tttttgtaat
    11641 actaatgttt tgtttgatct tttggcgatt tgtattagcc ctcgtaagtc tactattcaa
    11701 gatgattgca gccatatttt tcataggatt atttcgatga aaggagagag taccaatgcc
    11761 aattgcaata ggaaataaac ggttacccgt gacgttggat gaaaagagac aaaaagaatt
    11821 gcagtaacca aagcagaagt acggcaaaag tgaatccagg attatgtgta ttgcgttaga
    11881 tttattgatt gcgcaagaaa aagcaggatt tgacatacca gcactcaaaa agtgatcgca
    11941 ctttgcgcac gtgttaatac gtgttataat tattttgtaa ggaggtatag tgcacaatgc
    12001 cacttacggg aaaagaaatg cttaagttat tgaaaaaaat ggctgggttg agcgaaggca
    12061 agagggattt catcatcatc tttataagga tggtgttaga atcactgttc ctgttcatgc
    12121 caatcaagat cttggccgag gacttgagcg aaaaatttta aaggatgcgg gattgaaata
    12181 atctcgcatt cgattaaaga aaggattgac tgaaatgtta agtgagcctt tagcaaaaac
    12241 ctatcctgct atttttagcc cagaagaagg tggtgggtac tttattgaat ttcctgatgt
    12301 tcaaggtgct tacacaggaa ttaacgaaga cgatatatct tatggaattg ctatggcaca
    12361 agaggtgtta ggtatggttt tagcagatta tattgagcat gaagatctac ttccagaacc
    12421 aacaccaatc aataagattt ctgttgaaga tgattctttt actactttaa ttcgtgtaga
    12481 tgttgccaaa tatttgaaag acacagaact tgtcaaaaaa accttaacaa ttcctcaatg
    12541 ggcggataag ctaggcaaaa gggctggcat aaatttttca gtccttctaa cagaatcaat
    12601 tgcagataaa gcagataaca ttcttcgttc tggaagaaat aattagaact caaaagggtc
    12661 agaaacttaa aaatctttgg cttctttttt tactacaaag atggcttctt taaattattt
    12721 gcctgattga tttaaaaaaa ttgcgcaaag actacgagct aattaagttg ctcgtggtct
    12781 ttttttatga aaacttttta gat
//
LOCUS       DAZUJO010000077        12738 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000077 DAZUJO010000000
VERSION     DAZUJO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.078"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8OL_002262"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8OL_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4818231.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8OL_002263"
     CDS             complement(323..892)
                     /locus_tag="ACG8OL_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS4818232.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8OL_002264"
     CDS             1078..1980
                     /locus_tag="ACG8OL_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS4818233.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8OL_002265"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8OL_002265"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS4818234.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8OL_002266"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8OL_002266"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS4818235.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8OL_002267"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8OL_002267"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS4818236.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACG8OL_002268"
     CDS             8548..8733
                     /locus_tag="ACG8OL_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS4818237.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACG8OL_002269"
     CDS             complement(8795..9991)
                     /locus_tag="ACG8OL_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS4818238.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACG8OL_002270"
     CDS             complement(10161..11597)
                     /locus_tag="ACG8OL_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS4818239.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACG8OL_002271"
     CDS             11890..12699
                     /locus_tag="ACG8OL_002271"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4818240.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2250 g         4012 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaatt
//
LOCUS       DAZUJO010000078        12505 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000078 DAZUJO010000000
VERSION     DAZUJO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.079"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      complement(21..87)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(129..776)
                     /locus_tag="ACG8OL_002272"
     CDS             complement(129..776)
                     /locus_tag="ACG8OL_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS4818241.1"
                     /translation="MEQTIKPYQYFFIKREVDQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(1055..1558)
                     /locus_tag="ACG8OL_002273"
     CDS             complement(1055..1558)
                     /locus_tag="ACG8OL_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4818242.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1681..2445)
                     /locus_tag="ACG8OL_002274"
     CDS             complement(1681..2445)
                     /locus_tag="ACG8OL_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS4818243.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     NAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2552..2827
                     /locus_tag="ACG8OL_002275"
     CDS             2552..2827
                     /locus_tag="ACG8OL_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS4818244.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3504..4454
                     /gene="yidC"
                     /locus_tag="ACG8OL_002276"
     CDS             3504..4454
                     /gene="yidC"
                     /locus_tag="ACG8OL_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS4818245.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4633..5607)
                     /gene="lacD"
                     /locus_tag="ACG8OL_002277"
     CDS             complement(4633..5607)
                     /gene="lacD"
                     /locus_tag="ACG8OL_002277"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4818246.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(5604..6062)
                     /locus_tag="ACG8OL_002278"
     CDS             complement(5604..6062)
                     /locus_tag="ACG8OL_002278"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS4818247.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIALDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIATLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(6059..7522)
                     /locus_tag="ACG8OL_002279"
     CDS             complement(6059..7522)
                     /locus_tag="ACG8OL_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS4818248.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGIKIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(7504..8454)
                     /gene="pfkB"
                     /locus_tag="ACG8OL_002280"
     CDS             complement(7504..8454)
                     /gene="pfkB"
                     /locus_tag="ACG8OL_002280"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS4818249.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPKIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFETWENIN"
     gene            8597..9346
                     /locus_tag="ACG8OL_002281"
     CDS             8597..9346
                     /locus_tag="ACG8OL_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4818250.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            9613..11091
                     /locus_tag="ACG8OL_002282"
     CDS             9613..11091
                     /locus_tag="ACG8OL_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS4818251.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(11159..12250)
                     /locus_tag="ACG8OL_002283"
     CDS             complement(11159..12250)
                     /locus_tag="ACG8OL_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4818252.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT         3900 a         2533 c         2110 g         3962 t
ORIGIN      
        1 aagaaagggg ctatccccag actttagtag gcgtcattag ctatctttcg ttagcattta
       61 aaggcgaaca ccatcaccta ttcacttcca ttaaaagata gcatattttt ctgtaaaaaa
      121 caataaaatt atcgttcttt ctttacacgt tcagcaaatt catagaaagg agccaatttc
      181 gttaactgtt gattacattg gtcactatca tgacaaatat aattcccctt tttcgtatac
      241 gtgccatcag aacctgcttt cgttgtagaa agaaaaagcg acacattcga tgttttctga
      301 cagattgcac agattccctt atttatcgtt ggtgataaag taccgctaat cccttttaat
      361 ttttcatctt cgtaatagac catgaatttt ttctgcgtac ctggatcatt ccagccgatg
      421 tacgtatgtt cttgcaagtc gatttcattc caagctggtt gtttgagttt tttcactttt
      481 cggaataatt tctcgatttg tggtttgcta ggctgcataa acggctggac gatttgcttc
      541 agctcctcca atgctctttc tgcttgcgct tttgtcagtt ttacgtctaa aactgtcgtt
      601 aaaaatgtat cgatttctgg cagttcgtgt tctaatatgt ctcgaatctt ttcagctgct
      661 agtgcctgaa ctgtttgtac cgtttttgga tcattgacag atgagtacgc atttaataac
      721 tgatctactt ctctttttat aaaaaaatat tggtatggtt ttattgtttg ttccatgatt
      781 ttaaagtcct ttcttctaac caaagtgcat actttgtaga aaaaagtgat cttctgcaaa
      841 gtatgtgtta cctgaagatc ccggactctt ttaaataaga tacgttttgc atcttatcat
      901 cacccccttt aatgttcatt cttctatgta catgattcat tcgataaata aaagtaaacc
      961 gtatcttgaa cagatagtca actgcaaaca gaaaaaaccg agaaaatcag ccagattttc
     1021 tcggaatata gtctattatt attggtttta cctattaaat cgaggattct ttcccaaagt
     1081 aacgttgcca tctcttacgc atacttgcag acatgggctg agccgcttct ttgactgcgc
     1141 agtatttgtc cacgaatgtc acaatatatc cttctttata cttaggcggt gcgatcgttg
     1201 caccccacat gtgcttcaaa atgatatctc gctcaagatc cgacaaatcg gtgatttttt
     1261 ctgcattctt tacagcaatt cttggatgga tatacgcatg tgtcccctcg tcaaatttcg
     1321 tcgtacgcca atcatagtag aacagatcat gcaaaagtcc agcacgcgct gttgctttag
     1381 cattgccacc ccattttttt gcgagcagat aactatagta agaaacactg atggaatgct
     1441 ccaaacgagt ggaatgaaca tgctgtgtgt aattcgctaa ttttttcact gcttctgttt
     1501 ctaaaagatc ttgtacataa gacatatatt cttcgtcttc acgccatttt tctgtcataa
     1561 ttatcgatct gccacctttt tgaatttcat tttcttctga aattgatcta agtataccac
     1621 cagtgttcgg attattctag tcgctctaga taaaatagct aaagcttaat agttccttaa
     1681 tcataaagaa aaatgataaa gaacgatccc tgctgcgact ccgacattca atgattccgc
     1741 atttcctttc attgggatat atacgttctg atcagtcaaa gacaagattt cctgacttac
     1801 tccctgacct tcgtttccca tgattatagc gaaatcctct gttttttcta ctttgtctaa
     1861 tgcatgtgct tcttcattta attctgtacc gtacaccgta aaccctttgc ctttcattac
     1921 agggattaca tctgccaaat ctttttgtaa cacaggcaaa tgataattgc ttccctgcat
     1981 gcttcgcagc acctttgtac tataaatatc tgccgttcct ttacctaata aaacaccagc
     2041 cataccaaac gcatctgccg tacggatcat cgttcccaca tttccaggat cctgtacatt
     2101 atctagtaaa agccagcctc ctgaaaaatc tggcgtatct tcagatgtta acgtcatcac
     2161 tgctaagatc ccttgcggtg taggtagatc tgacatagcg gtcatgacct catctgaaac
     2221 tagaaaagta gaaatctctt tgtttacagc cagccagtct ccccattctt tcatacctct
     2281 ctgtgtcaca aaaagttctt ttatttctgc attagacgac gcagcttctt cgatcagatg
     2341 gaacccttcc aaaagatatt ctgccttttc ttctctgtat ttctttttat gtagtttttt
     2401 taattctttg attcttggat tttttgctga ttggatctct ttcacattcc tcacctcaac
     2461 tgattatatc atggaatagg tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg
     2521 atttataaaa caggaaagaa aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg
     2581 acgtgtccag ggcgtgggat ttcgctatat gaccaagatg gttgctgatc agttaggcgt
     2641 gaccggaacg gtaaaaaacg aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga
     2701 cgacatcatc caaaaattta tcgaagaagt caagaagtct ccgagcccaa gtggacgagt
     2761 gcagtatgtg gatgtccaag aagatccgat gattgaggaa cgaaagaaat ttgatgtagt
     2821 gggataaggt tgtgaaaagg tcgcttagtt ttgagtatta aggaaaattt tgtgaaaatg
     2881 gctttccaca tttttgcata aatttgactt aataccgaga aactgacttt tgaacaccgt
     2941 cgataaggtt gtttttgaag cgctttgcct cgagtattaa ggagaaacat tttcaaaata
     3001 gcttctcata ttttgaaatg taatgactta ataccgagag gcagctgata aaacaccgtc
     3061 aataaggttg tgaaaaagct ggtctgcatc aagcattaag gactggttgt gataaatagc
     3121 tctccatatt tttcaacagg ctggacttaa tgccgtagat gcaagctttt gaacaccgtc
     3181 gataaggttg tttttgaggc gctttgcctc aagtattaaa gacaggtagt aaaaaagctg
     3241 atctataact tgcaatgaat ttgcttaacc catattttcc agacagaaca aaaaagtttt
     3301 tagtttttta aataagaaag aaacacttgg aatcactctt ttctttctaa gtatttcttt
     3361 cttgattttt tgtctttttt caaaattttt cgctacgaaa aagaacgaat atttatatga
     3421 aatttctatg aagttgattg aattccatgc gcttttttag tatagttaag tttgtgtaaa
     3481 aattatagaa gaggaagtat tctatgaata aatggaaaaa ctggttattg ggatccggac
     3541 ttgtcagtat cctgctcttt ttatctggct gtgtgcggat ggattcaaac ggaaaccctg
     3601 acacttcagg aatcgtttat cgagtactcg tccatcctat gggccaagcg attacatatc
     3661 tagttgagaa tttcaattgg tcttacggct gggcagttat tgcaatgact gtgatcgttc
     3721 gtatcatcat cttgccacta ggtatcagtc aatcgaaaaa aacaatgatc caatctgaaa
     3781 aaatgcaagc gttaaaacca caagttgaag cagcacagca aaagctaaaa gctgcaacaa
     3841 cacgtgaaga acaaatggca gcacaagcag aaatgcagca agtatatcgc gaaaatggat
     3901 tgagtatgac cggcggaatc ggctgtctgc ctctattgat ccaaatgccg atcttctctg
     3961 ccttgtattt tacggcacgc tacacagaag gtatccggga atcatctttt tacggcatcg
     4021 atctaggctc accaagtatg gtgcttgtgg ccatcgcagg ggtcgcttat ctgcttcaag
     4081 gatatatttc aacaatcggt attcctgaag aacagaaaaa gacgatgcgt acgatgctga
     4141 tcgtctctcc tgctatgatc gtcttcatgt caatcagtgc gccagccggt gttacacttt
     4201 actgggtagt cggtggtgtg ttcagctgtc tgcaaacatt catcacaaac gttatgatga
     4261 aaccacgttt gaaagcacaa gtggcagaag agttgaaaca aaacccgcca aaacaagtcg
     4321 tcactccaag aaaagacgtg acagaatcag caaagccagc taacaacaaa caagcaacaa
     4381 aacaaatcaa acaaccaaaa aacagtacaa acaacaaagg ccgcaacgca ggaaagcagc
     4441 agaagcggaa gtagatagta aataagagtg taataaaaat tcattaacag ttttagcaat
     4501 taaaaatatc gaccaagatt tttgaaaaag aaatctggtc gatattttta tttaagtgaa
     4561 tgtttaattt gaatcatcga ttgatcgcta tctaattgca gcagcatctt tttatgaaga
     4621 aacaactttt tcttatctat caacgtagac aggtgttgct gtttcttcta atagtgtact
     4681 taattcttta atattttgtt ttccttcttc taacatccaa ctactcgctt tttcttcgcc
     4741 ttcggtaaca aaaggtgcca ctcccttcgc ccatgttgct cgcccacata agacaccatt
     4801 gaatgtcgaa cctgattgtt ttgcaaaacg taatgtatct tgaaatagcg tagcattgac
     4861 tcccgcactt aaaaaaataa acggttgttc agtcactttc gattgttcat agaaatacgc
     4921 ggatgcctct gcttgactat aacaagtctc ttgcccatat ccttccacaa atctcatatt
     4981 cactggcact tctactttca atacatccac ttgataacgt tcttttgaaa attctcgcat
     5041 ggcttcaata actttatgtg gttttacttt tgcataggca agagaagacg gatcgccaat
     5101 agttgcatca taagaaagaa tctctaaaaa gaaaggtatg ccttctgctt cgcattctga
     5161 accaatccgt tctataacta tttgtttttg cacattaatc tctgctggtt catcaacgtc
     5221 atagtacaat aaaaatttac aagcatccgc accagcttct tttaaccttt tcaccgacca
     5281 aatattcaat aaatcaggta atcttcccgg tattttcgta tcatagccgg ttttttcata
     5341 agctagcagt aagccacatt ctttctctcg aagcgctgct gcaggcaggc cgtattctgg
     5401 atcaagtaaa atagctgagg catagggagt caggtattga gaaaccatct gtttaaattg
     5461 gatgatgtct tctgatgtag cttctttata ttggttgatc attttcttca aagcaccacg
     5521 ctggtcgatt gctaatgcgc taataattcc agcttcatcc gtcaatcgct tcaaacgttc
     5581 ctgtttttct atactcatct tcttcataga tcttcaccta tttctttagt taatattgtg
     5641 gctatttctg tttttgattt tgcttttctc aactgttctg taacctcttt tcgcatcaat
     5701 gatcgtgcaa cgagagacaa taatttcaaa tgagtcgttc ctgcttctga atctggaata
     5761 aataacgcaa aaataaaatc aactgactgt ctatctaaac tgttccattc aattcctttg
     5821 ttcagtgtgg cgataatcat actaggttcg ttgattgttt gagacttcgc atgagggata
     5881 gcaaaacctg acattattcc tgtagtccct tctttttctc tttctagaag tttttgatat
     5941 acttcttttt cgtcaagagc gatccctaaa cagaatgttt gctttgctaa aaacttaaat
     6001 gcttcttctt gactatccaa tggctgttgt aaaaaaatgt tctcttcatg aatgattgtc
     6061 atgataagtc tcctgctctt cctttttcga taactgtacg atttgctgct ttattatcca
     6121 tcttcttgtc tttatcagtt actcttttct tttggattaa tcctagtatc actccaccaa
     6181 cgatcgaacc aataagaatc gctgtaaccc attggatttt tcccgtcact acaggtaaca
     6241 ctaaaaaacc accatgtggt gctggaactt ggacgccaaa caaataagtt aagacagcag
     6301 caattgatga gcctaacatc atcgtgggta acactcggat cgggtctttc gcagcaaacg
     6361 gaatagctcc ttccgtaata tgagttgatc ctaaaataaa attaaccaaa cctgcattac
     6421 gatcattggt atcaaaatat ttaccaaaca ataaagtagc aaaaccagta atcaacggtg
     6481 gtgcaataca agctgctgac acaccagcca taaaagtcgt gtttccttgt gccaaaagag
     6541 ctgttcctgt tacatatgcc gccttattaa ccggtcctcc catatcaaac gcacacatac
     6601 agccaacaat cacgccaaga acaagtggat tcgaatgttc aaatccagct aaaaagctca
     6661 tcattccttc atttatagcc ttcattggac ctgccagcaa agacatcacg atgccaataa
     6721 atgctacacc aataactgga tataagaaga ttgccttcaa gccatctagt tgtttaggta
     6781 aaattgtcag tagcttttgc aaaaataaga taaaataacc tgctaagaac ccagctaaaa
     6841 tacctcctaa aaatccagta ccgccattca tggctacgat tccaccaaca aatccaacga
     6901 ctaaccctgg acgtttcgcg atcccttctg caatataagc tgctaaaact ggaaccatca
     6961 ttcccattgc tgcatcgcca actgctttta ccattgccgc tgtagcattg tattgtgcat
     7021 gatctggatc aaaagaatag attccccaaa cggcaaatga aatagcaatc aatactccgc
     7081 cagctaccac gaaaggcaac atatgagaga caccgttcat taaattttta tagatgctac
     7141 gaccaaaatt agcaccactc atcgcttctg gttctaaagt atcagcagct tcattatctg
     7201 ctaagacagt accttttcct gccaatgctt cgtcaatcag ccgttgtgca tccttgattc
     7261 ctgtactgac agaaacatca atgactcgtt ttcccgcaaa tctttctgaa tggacatctt
     7321 tatctgcggc aataatgaca gcatctgcct gtttgatttc ttcagccgtt aattcatttt
     7381 cgatcccaat ttgtccatgg gtctccactt tgatcttgat gccttttttc ctagctgctt
     7441 gttctaatgc ctcttgcgcc atgtaagtat gcgcaattcc tgtcggacaa ccggttgctg
     7501 caattagttg atattttccc atgtttcaaa cctctcttcc ttgattttga tttgtttact
     7561 taattcctcc acatccaaaa aatcagtcag tcctttacga aaagcggttg agctgcctgc
     7621 tgctatgctt ttacgtaacg tttcatcaag cggtcgtcga ttcatatatc ctgctaaaaa
     7681 agcgcctaac atggtatcac ctgcacatgc tgtattgaca acttttcctt ttggcgagtt
     7741 tcctcgaaac accctgtctt tcgtaaaaag aatcgctcct ttctcaccga gggataacaa
     7801 aacattttct gcacctaact ctactagtcg ttgtccataa acaaagtaat cttcatctgt
     7861 aaccattttt cgtccgaacc aagaagaaag ttcttcctcg ttcggtttca gcaaaaacgg
     7921 tcgatagggt aaacaatcca tggcttcttg atcactacta tcaataatca aatgaatccc
     7981 cttttcctga caaatccgac tgatttctat taaaaccttt ggagacagcc ctcttggtaa
     8041 actcccagaa acacaaagat aatcttctgc ttgtaatgct cttatttgat ttaaaaaatt
     8101 atggacagct gtctgcggta ttttaggtcc ttgattcact aatttgtatt cttctcccgt
     8161 ttgattgact tgtgtaaata cattgatacg cgtcatacca ggcacttcaa taaactctgt
     8221 ttggatttgt ttctgctgta aatagtcttc tatatacttt cctgtaaacc cagcagaaaa
     8281 acctaatgcc gtacttggga aatctagcat ttttaatact agtgacacat tcaccccttt
     8341 tccgtttgcc tgaatgtcat cctcttttgt gcggtttacc ataaaaggat gcatctcctc
     8401 tgtttcaata aatagatcaa ttgccaaatt catcgtgcat gtgtagatag acataaccaa
     8461 cgacctcctt gactctatta tgaaatacgc gaaagagaaa atgttttctt aatcgtccca
     8521 acacaaagaa aacctttaca tttataaact cgtcgtgcta ttttttaact aacttttggt
     8581 gaaagaagga aagaatatgg atcacaaatt aagtgaaaca gaacagtacc tctggaactt
     8641 tatcgaacac cacatcctag agatccctaa ctactccatc gtgaaactaa gtgaacaagc
     8701 aaatgtctca acagccacca ttgtgcggac aatgaaaaag aaaggctatg aaggatttac
     8761 ttcgtttaag caccatctaa aagaaggaga aaacaagaac atcaattttt ctttcttaga
     8821 taaagtagac aaaggtatcc gtcgagccat tctaaaaaat gaacaagaag tggtacgtac
     8881 gatcaatatg ttggaaatcg gaaatattga ggatgcaata caaagaatca aatttgctga
     8941 ccgggttttt gtctttgcac gaggattttc agaaatgatt ggtcaagaga tgctcgtaaa
     9001 acttcaatta acagggaaaa attgtcaaat gcatactgat ccagaaatca tcaaaagcat
     9061 cagtcggaaa ctaactaaga aagacagcgt tatttttgtt tctttgaacg gcgagacaaa
     9121 agaactcgtc accgccgcaa agaattgcta tgatgcagag attggttcga ttctagttac
     9181 agccaatcat tcctctacac tgaaagacta ttgcgagatc aatctcgtag gtttcaaatc
     9241 agaaggttct tatttccctg attatgaagt acggtcacgt ttgcctctcc agattatagc
     9301 gagaatcctg cttgacgcct atgtattgcg tatgggggag aattgaaaag cagtttatta
     9361 tggttggtta cagtttaaaa catcacattt tttattcttt atattgtatg gactcacgga
     9421 aactttttat gtataaagtt attcacaatc tataaagtta ctttaaataa caaaaaaata
     9481 attgaaaata cttaaaatca aaaaatatct ttttaatcaa taaaaaaatt gacagtttta
     9541 ttaataataa gctattctgt tatagaagaa aaaattgttt gttattatta gcaacaaaaa
     9601 aaggagtgca tcatgaaaaa gttatctcaa aaacgtttag ctcaaatcat tattgattct
     9661 agaaaatctc tgaaaatgac acaacaacaa cttgcagtag ctactggtat caaccgtgcc
     9721 ttaattagtc gattagaatc tctcgatttt atcccgtcaa tacaacaatt agaatcatta
     9781 gcagaagttc taaattttga accaacagat ctatttataa atgaatcaaa taaatcaaac
     9841 agaacaaaac atcagtcgaa gaatattgct gtagccggta ctggttacgt aggtctttcc
     9901 attgctactc ttcttgccca gcacaatcag gtaacagcag tagacattct tcctgaaaaa
     9961 gtaaaacaaa ttaacaataa gaaatctcca atacaagacg attacattga aaaatatcta
    10021 gctgaaaaaa atcttaattt aattgcaacg actgatggtg cagccgccta tagagaagcc
    10081 gattttgttg tcattgctgc tccaacaaat tatgatagta aacaaaatta ttttgatact
    10141 tctgctgtag agtcagtaat caaattggtg ttagaagtca atccaaaggc aataatggtc
    10201 atcaagtcga cggtacccgt aggctatacc gaaagtattc gtaaaaaata caatactaaa
    10261 aatattctat tcagcccaga atttctcaga gaatctaagg cattatatga caatctatat
    10321 ccttcgcgaa ttattgtcgg cacggaccaa actgacaaaa ctttacgaga agcctctcac
    10381 acttttgcag atttactcca agaaggagca atcaaaaaag atatcgaaac tcttttcatg
    10441 ggattcacag aagctgaagc agttaaatta ttcgctaata cttaccttgc acttcgagta
    10501 tcttacttca atgagctgga tacttatgca gaaatgaaag gactaaatac acaagaaatc
    10561 attcacgggg tttgcttaga tccaagaatt ggcacacatt ataataatcc atcttttgga
    10621 tatggaggct attgtttacc gaaagataca aaacaacttc tcactaacta taacgatgtt
    10681 cctcagaata tgataagtgc aattgtcgat agtaatcgta caagaaaaga ctttatagca
    10741 gatcgagtgt tagaacttgc tggcacgtat gaggctaaca gttcgtggaa tgcagaaaaa
    10801 gaaaaagagg tagtcatagg tgtataccgt cttacaatga agagtaattc agacaacttc
    10861 cgtcagtcat ctattcaagg agttatgaag cgaattaaag caaagggggc aactattgta
    10921 atttatgaac caacacttga aaataacact acattttttg gttcaaaagt aataaataat
    10981 ttagatgatt ttaaaaatat cagtcacagt attattgcta atcgttacga tagtaatctt
    11041 gatgatgtaa aagaaaaagt atataccaga gatttatttc aacgagatta aaaaataaca
    11101 cctcagattt gtagtgtctg aggtgtttct ataaactttt ataagtaaaa aaatacaatt
    11161 atacttcata aaaagactta taccactgcg caaatttacg aagcccgtca cgcaaacttg
    11221 tacttggttt aaattcaaaa tctcgttcta acgcactagt atctgcataa gtaactggca
    11281 catctcctgg ttgcattgga actagttcgg tatgagattc aaagtcgtaa tcttctggta
    11341 gcacccctgc tgcaattagc tcttgttgta gaatatctac aaaatctagt aggttttccg
    11401 gcgtattgtt accaatatta taaatatgat atggtgggat tggtagccca tcttccccat
    11461 caattttctc tggagcgcac tgcataaccc tctttactcc ttcaacaata tcatctatat
    11521 aggtaaagtc acgcttgcaa ttcccataat tgaatatttg gatattatca ccagcacgaa
    11581 gtttatcagt aaaaccaaaa taagccatat ctggacgccc agcaggacca tatactgtaa
    11641 agaaacgtaa ccctgtagac ggaatattgt aaagtttaga atatgcatgt gccattgatt
    11701 cattactttt cttagttgct gcatacaacg atacttgatt gtctacttta tcctctgtag
    11761 aataaggaat tttcttatta gtaccataaa cggaagacga tgaggcatac actaaatgtt
    11821 ccacaccaga ttctccatta tcatatgaat gacgacaagc ttcaagaata ttatagaatc
    11881 ctattatgtt agattcgata tacatatctg gattagtaat tgaataacgg actcctgcct
    11941 gagctgctaa attcaccacg atacttggtt tataattatc aaagatgaaa tcaattgttg
    12001 ctttatcagc aatagaacct ttgataaaat tccaagtaga tcctggatgg ttctttgcct
    12061 ccgtttctat ctctgataat ctatagttct taatagagac atcatagtaa tcattcatat
    12121 tgtcaatgcc aatgattttt attgatgact gagtacgtaa taactcaaga accaaatttg
    12181 atccaataaa accggctgcg ccagttatta atattgttct atttgataag gacgtgttta
    12241 attccagcat tttttagcac tcactttctt tttttcttat gaatacatat ttgttatata
    12301 ttaaaaaatc ttatttagac ttatctattg tcacttttat cattctaatc tttgcacgta
    12361 gtagaataat tatacagata ggaaattgct aagcaaccca cttctttcta ttatctatta
    12421 acttattcta aaacgaatag aaattattct tgatcacata agtaacctta aaagaaaaaa
    12481 tttaatatat gtgtctaatt ttggc
//
LOCUS       DAZUJO010000079        12226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000079 DAZUJO010000000
VERSION     DAZUJO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.080"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACG8OL_002284"
     CDS             <1..566
                     /locus_tag="ACG8OL_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS4818253.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            967..1572
                     /gene="fic"
                     /locus_tag="ACG8OL_002285"
     CDS             967..1572
                     /gene="fic"
                     /locus_tag="ACG8OL_002285"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS4818254.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1588..2160
                     /locus_tag="ACG8OL_002286"
     CDS             1588..2160
                     /locus_tag="ACG8OL_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818255.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2412..2669)
                     /locus_tag="ACG8OL_002287"
     CDS             complement(2412..2669)
                     /locus_tag="ACG8OL_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818256.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2795..3610)
                     /locus_tag="ACG8OL_002288"
     CDS             complement(2795..3610)
                     /locus_tag="ACG8OL_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818257.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4127..5452
                     /locus_tag="ACG8OL_002289"
     CDS             4127..5452
                     /locus_tag="ACG8OL_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS4818258.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5445..5795
                     /locus_tag="ACG8OL_002290"
     CDS             5445..5795
                     /locus_tag="ACG8OL_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818259.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5792..5962
                     /locus_tag="ACG8OL_002291"
     CDS             5792..5962
                     /locus_tag="ACG8OL_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818260.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACG8OL_002292"
     CDS             complement(6866..7141)
                     /gene="prgO"
                     /locus_tag="ACG8OL_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS4818261.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7113..8066)
                     /locus_tag="ACG8OL_002293"
     CDS             complement(7113..8066)
                     /locus_tag="ACG8OL_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4818262.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8678..10171
                     /locus_tag="ACG8OL_002294"
     CDS             8678..10171
                     /locus_tag="ACG8OL_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS4818263.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10306..10602
                     /locus_tag="ACG8OL_002295"
     CDS             10306..10602
                     /locus_tag="ACG8OL_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS4818264.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10703..11554
                     /locus_tag="ACG8OL_002296"
     CDS             10703..11554
                     /locus_tag="ACG8OL_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818265.1"
                     /translation="MKSDDWIEHNWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11575..11787
                     /locus_tag="ACG8OL_002297"
     CDS             11575..11787
                     /locus_tag="ACG8OL_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818266.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4206 a         1872 c         2286 g         3862 t
ORIGIN      
        1 attattttaa gtctgaaaag gatacatcgt ctctaacgtt aactttagag gaaggagttg
       61 aacctgacaa ggtgacctct aaagtgataa accaattgaa agaaaaaggt agtttgaaac
      121 atttggggga atatgaagtg ttagatacgg ctatgttaac gaaaggaatt ggtcaaatcc
      181 tcagtacgat cacttatttt ataacagctg tagcaggtat atctttattt atcgcagggg
      241 taggtgtaat gaacatgatg tatatatctg tatctgaacg aacaaaagag atcggtataa
      301 gaagagccct aggagctact cgaaaatcga ttatgctgca gttcctttta gaaggtttga
      361 ttttaactct ttcaggaggg ttaattggct atttattagg aatggctctt gcttatagtg
      421 ttggttcttt gataaaggta aacgtgtcac ttgatctatt tactatttta ctagcggttg
      481 gggtatcttc ggttattgga ctggtctttt ctgtaatgcc cgcttcagag gctgcaaaaa
      541 aagatttaat tgatattctt agataaaatc agaatgacaa gaatatttac gttctagatt
      601 ctaaaataaa acacaaagag accaaccagg caccctattg tttctttgta gtatagagat
      661 attctttggc ttgtaagaga tcaatagagt taggtgaccg gagttcgaaa taaaggaaat
      721 ttttgattgt ataggagaat tctttatccc taatatttag aattttgagt atgccatgtt
      781 gatgttttag attctctata aatatagagg tgtatcaaag gtcaactaga ctgtgcaaaa
      841 tagagacact tgaaaaatct atcgggggtc agaaacttaa tagtttttga cacataaaca
      901 aaatcgcgat ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga
      961 tttcgcatga ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt
     1021 gaagaaaaac tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa
     1081 atcgaagttg gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt
     1141 tacgattttg ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca
     1201 ccagttatgt atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt
     1261 gaagagatta tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat
     1321 ggccgaagta ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta
     1381 gattggaact taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac
     1441 gatttggaaa tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag
     1501 ttatatatga aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata
     1561 gatgatttgt aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc
     1621 gcttctcaaa gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag
     1681 aatatttttt cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg
     1741 ttggctggtc tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt
     1801 aacacgagag aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag
     1861 gttttgaatc taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg
     1921 ttgagtgtag cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg
     1981 tttgcaagaa agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg
     2041 aagaagaggc acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct
     2101 ataaccggtt tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa
     2161 aaaagaagtc tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat
     2221 tagcatgttg tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta
     2281 tggtaactgt aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat
     2341 accatcatat atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc
     2401 gtaagtcacg tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac
     2461 tctattgatt ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag
     2521 aaccaaataa ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt
     2581 gtgatattac ctattgattt taccataact acgattaaag taataatggc attcaagtct
     2641 cctttgttct ctatatataa attagccata aatcatcttc ctttcaaatt aagatacttg
     2701 tttgatacca ataaatagta agcgtggcta gcataccttt ttctgtaata gcaagtgttc
     2761 taatagtcca aacttaatga tggaccgatg ctagttatag tacagtaatg ttttctttct
     2821 tatctagatt tttagtaagc tctatcagca aatctaacaa atctgagtcc ttttcagaaa
     2881 ctaaattaaa tctagtgttt acaacaaagt tttcatgttc tatattgttt agattatttg
     2941 cggaaaggag ggcttctaga tcttgacatt caaatctata tgcatcgtca taaatcttac
     3001 tagaaggagt gcttaattcg aaattactta ttttctcatc ttggaccaag ataaatttat
     3061 agatagtatc tttttgtgtt tttcttttaa aaacacaaca gagtgtgtta taaggaacgt
     3121 tataattttc atcagcctca ttttttctcc attcaaacaa atgataaact gcaaccaagt
     3181 tgccatcaat ttcatggaca gtgctctcgg taacatcctt taaagaacta tcaagggcta
     3241 atttatagta actagagaga ggaagtaaaa ttgtcgattt actgagatgg ttatcgtcta
     3301 tacgtgcaat catacctaaa tcaactaagg ttttattcca ggtgtttata gttttagttg
     3361 tcactcctag agactctgct atggtattga ccgaatgcca ggaatccccg ccttcattct
     3421 ttgccttaaa acagtaatac agatataaat taatagcttt gttattcggt aaatattttt
     3481 caagttctgt tggtagaata aagtaagaat ccttaatgtt ttttctgaac tgtctccagt
     3541 ctctgtagtt ttgtttataa agttgtgaaa cggtttttcc acctttagaa agtcctgcta
     3601 tcttaaccaa tcttattgct cctttcttga atctgtcatg atcatacagt attttttact
     3661 tgtagtccag caccttaata tattatccta tgtaattaat ttcgtactag atagtaatta
     3721 ttttttattt aattctctag tcacttacaa ataagttata taaactacga ttaataaatt
     3781 attacaaaaa ctattaagaa aacttctaca tacaactatt acaaaactat taagaaactg
     3841 aatactccta ttttttgttt ttaaggaaag ttttttcttt tttgaatttc ttattttttt
     3901 ctaatataac taagcaaaac tggataatac gacacttatt ttcaaatttt cacaagacta
     3961 atagtaaata tccatttaat aaagtgatac aatgtgttga ttttacaaat caacagaaga
     4021 agtagggaaa taacttacct atgcttcttt ttttgtacat ttaatcgatt cttctttaca
     4081 aaaacgaacg tatgtttgta taatgtggaa gaatggagtg ttatttatga tggattattc
     4141 aaaagagcca gtaaatgatt actttttgat cgatatgaaa agcttttacg caagtgtcga
     4201 atgtatagaa aggaatttag atccattaac gactgaatta gtcgtcatga gtcgagcgga
     4261 taatacagga tcagggttga ttttagcttc ttcaccagct gcaaagaaga agtacggtat
     4321 ttctaatgtc agtcgaccac gagatctacc gcaacctttt cctaaaacgt tacatgtcgt
     4381 tccaccacgc atgaatctct atattaagcg aaatatgcag gtaaacaata ttttcagaag
     4441 gtatgtagca gacgaagatt tattgattta ctcgatcgat gaatcgatct taaaagtgac
     4501 cagatcaatg aatcttttta cgactgaagg aacaagaagt caacggagaa agaagctggc
     4561 tcaaatgatc caggagaata ttaaagaaga gctaggattg cttgctacag taggtgtcgg
     4621 agataatccc ttattagcta aacttgcttt agacaatgaa gcaaaacata atgatggatt
     4681 ccttgctgaa tggacgtatc aaaatgttcc tgataaagta tggaatattc ctgaaatgac
     4741 agacctttgg ggtattggat ctcgaatgaa aaaaagactc aaccaaattg gtatcatgag
     4801 tattaaagac ttagctaatt ggaatccata cactattaag agccatttag gtgtgattgg
     4861 cttacagctg tattttcatg cgaacgggat cgatcgaaca gatatagcga tcccaccaga
     4921 accgacaaag gaaaaatcat atggtaattc acaagtgtta ccacgagatt ataccaggag
     4981 aaatgaaatc gaactggtgg taaaagaaat ggcggaacaa gtagctattc gtatcaggca
     5041 acataactgt aaaacaggtt gtgtccattt atacattggc acgtcgatct tagaaacgag
     5101 accaggtttt tcacaccaga tgaagatccc tataacagat aatacaaagg aattgcagaa
     5161 ttactgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc acgttggaat
     5221 aacctatagc aagcttgtgt acactgattc gcttcagctg gatttattta gtgatccaca
     5281 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
     5341 ttttacgtcg attgtacatg cttcttctat gttagaaggc gctcgtagta ttactagaag
     5401 tacattggtt ggtggccatg caggcggcgc aggagggctt aaaaatgatt gattataatg
     5461 atcgtggacg tttaaagtgg gcaggttttt atttatctga tcatactgaa aaaattgatg
     5521 ccgacaatga acaacgagcc aatcaaaatt tggcaaaaga acaaatgacg actgaagagg
     5581 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatgtatt caaaaagaag
     5641 aacgaaacgc tgaaggatac tatccagaag atattgttag caagatctta ggtagtgatg
     5701 agctggggat ctacgttgga aaaaacaaag ttcactacga cgaaatacgt catatcgaat
     5761 tttaccagga taataaatgg ggtcgattag aatgaacatt atccagcaat atgagctaaa
     5821 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccagc gtttgataaa
     5881 tgaggttggt gttgaacgct tctctttcta tgtagaagct gcagcaggat accataaata
     5941 cgaatactat attggctatt aaagtgcaat aaaattatat ttcttttatg ttttacattt
     6001 tgtatcaatt ctgataaaat tacctatagg taattaaaaa atataaaaaa actagttaca
     6061 accttaggac gttgtaacta gcttgaagca tactagagct aactaaaata gcactagaaa
     6121 gcaatgttaa acccgtccaa caaacttgat ttaacattga gaccagctta ttcagctaaa
     6181 tatagttgaa taagcttctt ttgttttaaa aaaacaaaag gtgtactacg aataacgcaa
     6241 gtaaaagaaa tactattaca accagtaggc tacaagtaag tattactaaa cattgcgtag
     6301 ttagaacctt tgctctattg tctaaagcca caaaattaga gatcagcttt atgatgctaa
     6361 gagcgagctg catccatgcg gacataggtt cttttgacac tattaattct cctttttagg
     6421 ataagggaaa ttgctagtag ttttttcatt agcaccatcc cccttcattt gattgagtct
     6481 agtgctgctg tgatagtttg ctgtttaata tgcttacatt cttattataa cataaatgaa
     6541 acaaacacat ggttagaggt cgtatcaatc aaaatagtgt ctcacaaacg atttattact
     6601 tactcctgct gactaacgga ctgttcttca gtaactgtaa ctatatgttt gttaaaacca
     6661 ccattacctg tagataaaaa ccaatatatt tactatactc actatattaa atatatttag
     6721 tatagtaaaa actagaaaca ataaaatttg tgaatgttgt atacccccaa acaatacaca
     6781 tattttgaag gattaggatt aataaagcta aaaatatagt aagtatacta aatatattta
     6841 ctatatctag tatacttact atattttaat cttttgatcg tctattatat ataatccgtt
     6901 cctcgtcgtt taaagtatca acataggcgt ccattgcaag tttgactatt tcatacatag
     6961 gaatatcttt tgcataacta agatcccgaa tggtttttag tacgggagga tctacttgaa
     7021 ctggttttct ttctttcgca gtgtactttc gttcccctaa ctcgacatta atatcatctt
     7081 taattgaaat atcttcagca ccaattttct tcttatcttt gttcgttggt aataatgcca
     7141 tttagtttaa tctcctttcc aagaggtagg atatgattgg ccattgagtc agcatattta
     7201 ggtgtataat caaatccttc aatatcacca tgttgttcat agtattcaat tctttcttct
     7261 aattctgtaa agatgtctgc aaaaatagcc caccatcttc gatctacata atcatttaat
     7321 tggataccat tctcaccata catttctaac cgatccgagc ctttaacaat gcttttaaaa
     7381 atgtttgatt caccatactt atcaattgtt ttttgatagt ttgcgtgttg agaggaacgt
     7441 cgcactgaga aaagaacagg caaaattcca atgatttgaa gatgagactc ttcaaaattc
     7501 gtgacaatcg gaactaaaac tttattaata aaatcttctg ttccttccat tgcgtaccgt
     7561 tttaattctt gcattgcaat aatataatct gcagcagtta agaatgcacg aaccacatta
     7621 tctgttgatg gtgggcagtc gataaagata taatcgaaat tttttctcaa ttcttctaaa
     7681 ggtttaatga aggccaatga tctcttacga cgatcaggat atttttcgga caagtattca
     7741 ttaagttctt cagatccagg agagccagcc ataatatata aatgtggact tacttgagta
     7801 atacctgaaa cccagtcccc ttttttgatt gctgcagaca tggatcgatc aaagctagta
     7861 atttgatacg ttgatccaat gttggaagta gtatttcttt gttggtcata atcaattagc
     7921 aacacgtctt tttcaaaaag ttgactagaa acaatcgcta ataaatcatt gtctgtagtc
     7981 ttaccaatgc cgccttttct acctgtaagc attacaactt ttcctagttt atctgatgtt
     8041 ttaaatgcag tccgtaagtt ttccatagtt ccctccaatt tcctatacta aatatattta
     8101 gtatacttca tatacttaat atattcgata tatctaatat actccaaatt gataagaatt
     8161 cagactttat aaggaatata gtggatatac taaatatatt tgctatattc agtatatcag
     8221 atatattcaa aatgtcaaaa tatatttgtg cgttttgcgt tgacaattta gagtgaaaag
     8281 aggtaaactt aattctatag aaaccacaga taattcacta tttacaaagt agcaaaagca
     8341 gtggtactct ttaaacagaa aaattacata aaaaaaagag acggaaataa ttgattttag
     8401 cagaatcaat tatttctgaa tcacatggtt aggacatgtg ttcacgctcg tctcaagaat
     8461 atgtaagtag ctaccaacta cttatattaa aagttgatcg gatacctttt ccgattgcct
     8521 ttattctacc aaaaaaaggc gagcaactca acagaagaac gtttatttag tgtacttaaa
     8581 ttaaatattc ttaaacactg aagagagaac acatactatg gctgagatta aagctaaact
     8641 gtgttctctt tttgtttgag aaaggacgtt acaatcgatg ttgagcgaaa tttattcagc
     8701 tattcttaaa aacggcttac gtacatacaa atatcgaaac agcaccatga aacctgcagg
     8761 tcatattgat tccagtagga gtggtgctat ctttgcgtac cgtagcaagc aattaatgaa
     8821 tgttgggcgt ggtatggtca ttacttcgga agaagctatt ttagagaacg aaaagaaact
     8881 aacgcattgg acacctaata cctatcgctt tggtacttat gcggatgatt atcgaacagt
     8941 ggttaaaggt cattctgaga aaaacttaag tcaaatcaac acatttgtag ttgatatcga
     9001 ttcaaaagag aaccatcaag gcgaaattat tttagcctgt ttagatcaag tgggctacat
     9061 gccaacgcta attttagaat cagatcatgg ttatcaagtt tattttgtac taaagacacc
     9121 aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat gtagcaaaaa aaatttcaaa
     9181 aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg gatctaggat gtaaccactt
     9241 cggaattgct cgattcccac aagaaaataa tattgttttt aaagaaatga actatcagta
     9301 ttcttttgct gactggattc aatggtcgat gaagcttgaa tcagatcaaa aaacaaaaga
     9361 agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa gaaattcgtc aagtagatga
     9421 accgtggttc gatatgttac ttcataaagc aaacatcgtc ggcggagaag gacgtcttgg
     9481 tcgaaataat gtgattttca cgctttcttt agcttattac tcttcaggtt atgcattgga
     9541 tacttgcgaa tacaatatgt tcacattcaa cgaacggtta caggaaccgc tatctgaagc
     9601 agaattagta aagattgtac gtagtgctta ttctggtaat tatcaagcag caagcagaga
     9661 attcattatt gaattgtgcc agcaatgggt ttcaccaacc ttaaaaaata atgatctgtt
     9721 cattcaacga cgtggctggt ggaaatttaa aaagccacga gatcaaagaa aatatagtca
     9781 taaaaatgaa tggaaagaag atctattggc ttatctaagc gaaaaaagtc ttgctagtga
     9841 gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa ctaacgatgc ccaagcgaac
     9901 acttgataag atcttaaatg agttacgaga ggagaataaa gtcttctaca aggtaacacg
     9961 aggaagaaat ggcggaatga tcattgcttc agtcaaaacg ttgttcgcga gggttattca
    10021 actaagtaaa gaaaagagac tagattattc aacaaggatc acagaggtat ttggactcaa
    10081 cgagagctta gtagaagaga catttagaca attatcaacc cccttgaacc ctcttaaaga
    10141 agtgaatctt tttgaagtag atacaggttg aaaaataaaa ggtgcaaatt gccttataca
    10201 atatatctat gatacttctg taggtgttgc tccctttgcc tagctacgtg taggtggtat
    10261 acttataact gcgtatgcag tcttatgtat catggaggaa gttgtatgtc gttaaaaaat
    10321 tatgtctatt cacatcctgt taatgtgttt atcattggtc gtttgggatt atctgtggaa
    10381 gtgttctgtg agctttatgg ttttaagcag gggactttgt cttcttgggt taccagggaa
    10441 aaaacagttg cttctttgcc gattgagttt cttcatgggt tgtccttagc ttctggggag
    10501 actatggatc aggtatatga ttgtttgtgt gtgttagagc aggagtatat tgagtataaa
    10561 atagctaacg agttaagaaa aaggaagaag tatatccagt agtagttttt atttacttaa
    10621 aaaattggtt cattgagcaa cctatcgctg ctaaagtagc aaaggaaaac gttaaaggaa
    10681 agaatttagg gaagaaatcg aaatgaaatc ggacgactgg attgaacaca actggcttcc
    10741 tctgaaaaga aatagcaaac gaaagaaatt ttgggtattg caattatggc cattaggaaa
    10801 actttgtagt tacggggcac gacttattct tgatttaacc gtttactcga ttggagtatt
    10861 tggaacggct tattacttga taaatgatcc caataggatt gcagcatcag acatttttac
    10921 agtgctaatg acgttgattt ttgtcatgct attgtctatt tttactcaat tagtcatgca
    10981 atttaccagg caaaaattaa cagcaggaaa acttttcaaa agcctattcg atttaattgt
    11041 tgtaaatgca attctagcga ctttaataaa acaaattgaa caatctggaa gaacaacaaa
    11101 aggagtgaag gattttttat tgacgtttat tcactttgta aatggacaca tgtatttgtt
    11161 tgaagtgggt attattctct tttttgtcgg gtatgtatcc acgaaactta ttaaacgttc
    11221 aattgaaaaa gaaattgaaa ttgacctgat tccgacactt cttggttatg agtacaatgt
    11281 tgattttgga agatatgacg aattgatcaa tacggatatc gttcacacga gtatccatga
    11341 atctactgtt aagggcatca gatctatttt aaaaaatggc caggaaatga aagcaataga
    11401 tcgaagaaaa gagatctaca atttttatga agtcgaagca agcctacgat atggcatcgg
    11461 aaaattgtgg ttagatggaa gtaatattcg tcaaaacaaa ccatacattt tttggaacag
    11521 tagtgattta gaacttccaa tgaaagataa ataattatgg agaatcgttt gaaaatgatt
    11581 cctctataca ggattgtgct tatagttggc atggagttaa aaaccttaaa gaaggagcag
    11641 ttattcaaag tgaaagaacg tcataatcag ttactatgta gtaaaacggg tacgatgaac
    11701 attgaatacc gaacagaata tttagaatta acgaatcaaa ctgatttttt gttgattcga
    11761 aagcaacaag agcaagaaaa aaattaatgg ctacttatag ccaattgatt gatgaatggg
    11821 aacaattcaa ggaatctaaa attccagaac gtctctaaaa gcctttattt gtaaagagca
    11881 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
    11941 tctcaaaaat aaatgtggga acaaaatatt taaaaatagg atagtgaaag gatagtgaag
    12001 gatagtaaaa ggagggtagt aggatagcaa aaaggatagg ctccttttcg ctatcctttt
    12061 taaattggag gaattaatat ggaagtaaga ataagaaatg tggatgagaa aatcgtaaga
    12121 gaaattgatc gaagatgtga cgaacggttc tgttgcaaag ttttaaatct actatcaaat
    12181 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatc
//
LOCUS       DAZUJO010000080        11733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000080 DAZUJO010000000
VERSION     DAZUJO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.081"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            406..675
                     /gene="rpsN"
                     /locus_tag="ACG8OL_002298"
     CDS             406..675
                     /gene="rpsN"
                     /locus_tag="ACG8OL_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS4818267.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            690..869
                     /gene="rpmF"
                     /locus_tag="ACG8OL_002299"
     CDS             690..869
                     /gene="rpmF"
                     /locus_tag="ACG8OL_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS4818268.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            901..1278
                     /locus_tag="ACG8OL_002300"
     CDS             901..1278
                     /locus_tag="ACG8OL_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818269.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1299..1448
                     /gene="rpmG"
                     /locus_tag="ACG8OL_002301"
     CDS             1299..1448
                     /gene="rpmG"
                     /locus_tag="ACG8OL_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS4818270.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1472..2455
                     /locus_tag="ACG8OL_002302"
     CDS             1472..2455
                     /locus_tag="ACG8OL_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4818271.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2485..2607
                     /locus_tag="ACG8OL_002303"
     CDS             2485..2607
                     /locus_tag="ACG8OL_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS4818272.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2625..4148
                     /locus_tag="ACG8OL_002304"
     CDS             2625..4148
                     /locus_tag="ACG8OL_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS4818273.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4172..4411
                     /locus_tag="ACG8OL_002305"
     CDS             4172..4411
                     /locus_tag="ACG8OL_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818274.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4604..5167)
                     /locus_tag="ACG8OL_002306"
     CDS             complement(4604..5167)
                     /locus_tag="ACG8OL_002306"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4818275.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5260..5478)
                     /locus_tag="ACG8OL_002307"
     CDS             complement(5260..5478)
                     /locus_tag="ACG8OL_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818276.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5667..6614
                     /locus_tag="ACG8OL_002308"
     CDS             5667..6614
                     /locus_tag="ACG8OL_002308"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS4818277.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6581..6670
                     /locus_tag="ACG8OL_002309"
                     /pseudo
     CDS             6581..6670
                     /locus_tag="ACG8OL_002309"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6803..7210
                     /locus_tag="ACG8OL_002310"
     CDS             6803..7210
                     /locus_tag="ACG8OL_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4818278.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(7451..8791)
                     /locus_tag="ACG8OL_002311"
     CDS             complement(7451..8791)
                     /locus_tag="ACG8OL_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4818279.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9229..9375)
                     /locus_tag="ACG8OL_002312"
     CDS             complement(9229..9375)
                     /locus_tag="ACG8OL_002312"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818280.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9406..9609)
                     /locus_tag="ACG8OL_002313"
     CDS             complement(9406..9609)
                     /locus_tag="ACG8OL_002313"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4818281.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            complement(9519..9851)
                     /locus_tag="ACG8OL_002314"
     CDS             complement(9519..9851)
                     /locus_tag="ACG8OL_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818282.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10365..10556)
                     /locus_tag="ACG8OL_002315"
     CDS             complement(10365..10556)
                     /locus_tag="ACG8OL_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818283.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10654..11040)
                     /locus_tag="ACG8OL_002316"
     CDS             complement(10654..11040)
                     /locus_tag="ACG8OL_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4818284.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3917 a         1829 c         2245 g         3742 t
ORIGIN      
        1 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
       61 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
      121 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
      181 agtgaattct tatttttcag agcttaaact gcatattctt ttaaatgatt tgattatctc
      241 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
      301 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
      361 acgatttgag atgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
      421 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtacgcaga taaacgtcag
      481 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
      541 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
      601 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
      661 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
      721 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
      781 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
      841 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
      901 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
      961 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
     1021 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
     1081 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
     1141 gaggagttat ttcaatgtat tgatggttat tgggagatac ctgttgatat gaaagaaaat
     1201 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
     1261 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
     1321 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
     1381 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt ttcaaagaag
     1441 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
     1501 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
     1561 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
     1621 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
     1681 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
     1741 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
     1801 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
     1861 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
     1921 catagcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
     1981 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
     2041 tgaaacgatt caaaaaaagg atttttataa tttggaaaaa ttcaatcatc ccttagtttt
     2101 tgaaagtgat tcatcagaaa atcactataa tggcaaacac tatcatcaac atcatcaaga
     2161 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttatt
     2221 ccaattaatt gaccggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
     2281 gattgctttg caagatcaag attttatagt tgccatacaa ggggtaaacg aacaggttaa
     2341 ttttcaatac accactcgac aaaaagacca aaccactatt attctaattg gaaaaatgtt
     2401 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
     2461 agtattgatt aggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
     2521 cttaaaagcc cgaatatcaa aactagaaaa agggcattaa aaattatcaa agaacataaa
     2581 caaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
     2641 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
     2701 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
     2761 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
     2821 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
     2881 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
     2941 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
     3001 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
     3061 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
     3121 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
     3181 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
     3241 tgacacaaca tgcagccttt ggatatttag ctttggatta tggattgata caagttccta
     3301 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
     3361 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaatcg
     3421 caaaaacttt ggccaatgaa gctgggataa aattggaagt attgaatcct cttgaaagtc
     3481 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
     3541 aagcgttaga gaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
     3601 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
     3661 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
     3721 atgaagtttt cgattacaaa gcaaaactca ataaagacat gacagctgcc gagtacaaag
     3781 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
     3841 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
     3901 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
     3961 gtgctggtcg ttttaaatat attcaattta gcgatcatgg aattgcacca agtaaggcag
     4021 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actttataat gaaatgcata
     4081 attggcctac tttctatcca gcttcattat ctgagcatga gattgctcaa gaaatgatgg
     4141 cccattaaaa tccaatcttt ggaggaaata catggtaaga aaagtattat tgtctatgga
     4201 ttccaagaat attgatgctt atcggcgtta tcaatttgca aagcaaatga gattaaatgg
     4261 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
     4321 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
     4381 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
     4441 cttatttgga tttgatctgc tttttattat ttcttgaatt gtcaaatcaa gcgcaacact
     4501 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
     4561 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
     4621 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
     4681 gaggatctgg aaaataaaag ggcatattat tcaatacttc tgtaactgcg aaatgattgg
     4741 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
     4801 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
     4861 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ctagttttgc
     4921 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggat
     4981 ggccatggat cgaatgagta aggcaaatct tcccacgaga ctcgacatga ttttttgtat
     5041 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
     5101 gttctgtatc gaaaagatca ttgcaaattg cacggtagat cgtgataaaa ctaattgaaa
     5161 agtccatatg ttcatgcttc aaacgattcg agattttctc tggcgaccac tgaaaatcga
     5221 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
     5281 atttttttct tcgataagca taattttttt ccgctttgga aggtgattag tttttttctt
     5341 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
     5401 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgtttga gtatagtaag
     5461 atgacaatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
     5521 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
     5581 agaaagaggt tccctatgaa aaaataccta ttacaatctt aactggctat cttggttcag
     5641 gaaaaacgat acttctaaat catattctgt acggtgatca cggaaaggaa attgctgtca
     5701 ttgtaaatga cataggggaa gtaaatgttg atggggtatt agtcgacaag aaaggtttta
     5761 aaagaactgg agaaaaactt gtttcgatgc cgaatggatg catttgttgt acattacgcg
     5821 aagatttgat tgttgaacta gatcaattag tgcaattttc tgacttgaat caaattgtta
     5881 ttgaagcttc aggaatcagc aaaccaattc caattattca agcaattata ttagctaaaa
     5941 accctttaga cattgatttg acccaacgat tagagattgg tgccattatt atagtagtgg
     6001 atgtaaaacg tttttgggat aatttcgaaa cgggtgaaaa gattcaggaa agaaaagctg
     6061 aaggtaatgt agttgaagaa gaacaggata ttcgtggctt gttaattgat caattcgagt
     6121 gttgtaacat attactttta aataatagtg acttagtgtc aaccaagacg atttacaaaa
     6181 ttaacggtct aattgctaag ttacagtcca atgccaaaat aattaggaca attaaaggtg
     6241 aagtagactt atcgaaaatt ctaaatacaa atctttttaa tatagatgag acttcctatt
     6301 caatgggctg gttcaaagag ttagagcttg gctatcaaaa tcatattcct gaaacagaag
     6361 aatatggtat ctcaagtttt atctatcgaa atcggttact ccttgacgca caaaaatttg
     6421 cccatcttac ggataaaaat tttcctggca atattacaag agcaaaaggg tttctttggt
     6481 ttgcccaaca tagtgatttt tcgacgatct ttgaaacatc gggtagccat aaaagtattg
     6541 aatcattaaa ttattgggta gctagtttac ctaaaattga gtgtcaaatg attatacaaa
     6601 aaaacccgaa ttgaaggaag cctggcatcc gttgtttggt gaacgcatta ataaactagt
     6661 ttttattggt atgaagatgg atcaacaatg tattaaggaa caacttgagg gttgtttaac
     6721 tgattctctt aatgcagata atttccgaaa aatttcacca tttccatgga aaaattctaa
     6781 tatgtttagc cagatatcgt taatgaaatt tttaagaaaa gctggtgtca agataggcga
     6841 tgtcataatt attggcattt tgattattcc ctcttttatt cccttatttg tattctctat
     6901 agaaaaggag aatgaaacaa ctgaagatgc tatcaaatat gccatcgttc aaattgatgg
     6961 caaggaaatg gatcgcttcg atttggataa gatcaatcat aaactcgtga attatcatcc
     7021 tgcgaaggga caatataata ttgttgagat taaagatgga agaattcgag ttaaagaaga
     7081 taatagtcct gatcaaatcg cggttagaac tggatggatc tcaaaacccg gtcaaacgag
     7141 tatttgttta ccccataagt tagttattag tattgagaaa aaagaaagca aagattacta
     7201 catttactaa tctggaaata gatgaggagg tttttaataa gaaaaagata ttattattaa
     7261 taatagcagg ggctttatta agtgtgttaa gcgcttgtgg acaagaaaaa gaagcggtga
     7321 caacaggcac aagtatcttt aacgggacct gcaatggttt agttaacagt cggttagatt
     7381 acttattttg gctcttcgtc aatgagtgtt ggtgaagaaa ttcaatagaa taatcgagat
     7441 actcacacgg ctagacagct atcttgtcta gccgtgttgt tgttctggct ttagtcagtt
     7501 gggtagtgtt cttagttttc ttgaaatatt ttcctcgata taaaaagatt cgttctcggt
     7561 agttttgaaa gtttctaaag ccataggcaa ttcgttttag tactttgata tggttgttca
     7621 aacattctat agggccgttg gaatagggaa ctcgcaacgc tcgtttgatt tctgttcgat
     7681 atttcttgaa ggtcgtgaac actggttgat agtgctctgg caactccttg tagtcttctt
     7741 ttaggagcgc gacgaattca ggaacatctt gtcttctgat agcggaaaga aaaacctggt
     7801 agacttcgta gcctcgcttt aaggagtcat cataatataa tagccgatcg actatctccg
     7861 cttctgtcag atgatctcga aatgacggac gccagtagcg atgctgacca ttcaattccc
     7921 acgcattctt ttgaagtagt ctccagtatt tctttaagtg cttcgaccga cgatcccctt
     7981 ttttaaaggt cttcattact tgaattcggt ggttggtaaa ggcccgaccg atgtgttggc
     8041 taatatgaaa gcgatcggcg acgattcggg cattcggaaa acactctttc accaatgtgc
     8101 gatagggtgt atagaaatct gaaaccacta gacgtacttg ttcacggact tttctagggt
     8161 agcgcaagaa atagtcgcgt aaaggattga gttgccgatt ttccacaata tcaatcagtt
     8221 gatgggtctt gccgtccatc atgacaaagc tcatggctcc cgagacgttt ttcaccgatt
     8281 tgaattcatc gaaacacacc acttctggta acggctggtt caaagaaacg gttttaggcc
     8341 gaaggctgcg aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcaatca
     8401 aagacataga aataggctca cttaatagct caagaatggc ttgtttgact ctgcgggcaa
     8461 tggagcagcg acgatcgcta actgaatctt ctgctaaaaa agtctttcca cagtgtttac
     8521 atttgaagcg ctgtttgtta atccgcacaa tggtcttgta gttactgaca tcattcaata
     8581 agatggatac ttttttccag ccccatttaa tgatcgtttg cttattttga tagtggcaat
     8641 ggaagcattc ctctggttga taggtcaatg tgccatcaaa aacaaaacat gtttcgtcat
     8701 taatgcgctc tttgcgaaag gcatcttcat gaaaggtaag atttaggtct agaatatcta
     8761 gtaactcttt ggtaaactct gaataggaca tcttgaaaca ctcctttgaa tggttttcgt
     8821 cgactttatt ctaaaggatt tcaaggtgtc tgtttgcgtt tattagaaaa aagaaaaagt
     8881 attggcaaca gaaggcgtca ccaacactag atattataga tcccttattt tcttcgcttc
     8941 aaattgtcaa ggagtcatca taaattttgt atggattctt ttgtttgggt agtagtcttc
     9001 taaataatct tggattaaca ccttcatctc tattttactt ttatatttgt tggagtcgat
     9061 taattctcat ttgattgatc gatgaaacgc ttcaattact gcattgtcaa caggagtccc
     9121 agtttggctc attgaatgtg tgatgtggtg gtcggtcaac agctcttgat aaataaaaga
     9181 ccgatgctgg ctacctctat cggaatgaat taccgtaggc aattctcctt aataaatttg
     9241 taatggacgg acgacaatct cttttttcat gtgtgaatcc accatatagt aacgtagaat
     9301 acgagttgct aagtcaatat aggtggacaa atagaggcga ccgtcattac atggaatgta
     9361 agtaatatcc gtcacccaaa cagcatcaat ttggttctgt tcaaattatc gattcaacat
     9421 tttttttacc atttctttga ttgttttttc tctaccatga gaagatttgc gtcgatgaaa
     9481 tcctcttgtg cataaattca tttcacgcat aagaacagct acaacacgtt tattgatttc
     9541 gattccttca tcgtaaagtt tttgagtgat ccctggagat ccgtatcgat ctttgtgttc
     9601 gtagaacact cgtcttcctc ttttagtcgt ttattttcct gttcaagaat gtagaaatct
     9661 gctttatcag gtaatctttt tccttgtcca gcaaaagcat ttttcccgta tcgacgatat
     9721 tctgaaaccc atcgggaaat tgttggacga gagatattgt attcttcagc aattttcttg
     9781 gtagttactt ttttctcaag gatatcctgt gaaatcttta gtttaaattt acgatcatat
     9841 gtttttttca atagagttca ttccttccgg taacgtaact gatagtcaag aaaagtgtat
     9901 catagcccaa cttagtatca gggtttgtca actaaactgt ggaagttgat agataagagt
     9961 ttttaacaac caaaattctc ttgactattt ttgaaatcag acaaattttg attggacaca
    10021 tagttgaata aatctaccgt cgttacggaa ggtaaatcgc atgcttttca attctagagg
    10081 agaagtcctc gttgaagtac catggcccag ttctggattc gacctcctgc ccacttcgta
    10141 cgtaaataaa gtaattttag gagtgagact gtgacaaaag ttatttttaa gctaaaaacg
    10201 aattaaaaaa cgttaataat taaaatcatt cgttttagat tctatgaatc attatagaac
    10261 gctgtaaaat cgatacttta taattataaa gaagatggct tcaaatttat tgttcgaaat
    10321 ttgaaactat ctttttactt atgtcacaga ctcccttagt tgagttattc tttaatttcg
    10381 aatttcgaga tagctagata tattgcaagc atacattctt gataacaatc ttgatcatat
    10441 tttccagttc tccaggattg cttatggacc aaagcgctaa atcttttgag aatggccaca
    10501 actgcttctt catttccatt ctttgctaaa atgaaatctt cttttaaagt attcataaat
    10561 ttaccttctt cccatgaact tataatctat ttattttttg gggaaggagg aggacgagca
    10621 aagaagcata caaaattagt tattatgcag atattaaacc agcatttttt gaattttttt
    10681 gtaaagtcga tgttttaaat ttgtcgcacc ctgtcttgta atacccagaa cctttccaat
    10741 ttctttatca gttttattaa agataaattt ttcgataaga aaaatttgtt caggttcttt
    10801 caacaaagga aggatttttg ctaattttat atcttcaaca aggagagtca catttaaaac
    10861 attaatcggt tgtttaaatt ttatagtatc tttagtaaac aaatatggtg ggagttgatc
    10921 aagttgttca tgtttttgaa tttcttcttg ttttttataa taagtattag cggtgttcaa
    10981 aatgaccttt tcaaagaatc gaatcaactg ggcatttacg cgttcgtcgt tatctgacat
    11041 attgcacacc tcctccatta agatttaaag ggaaaagagg caaaaaacac gaaaagaagc
    11101 taatgcaatt atgctgcgct gcgaataact tctataagct cacatgcatt gataggactg
    11161 ctgatgttta ttttcaaaaa tgttttttta gttgataatg gatagttatc tttatactta
    11221 gaaaagaaaa ttcatatatc ttcttaaata tcaaacatgt ccttatggct aagaaaatgt
    11281 cttgagcttt ttacgtataa taaatctttt ctaaaacctc cttttggata atattcttat
    11341 tcgtgtatag attgtatttt ttatttcttc ccccttcata attaacaact agaaaagagt
    11401 tcgaatagac acgtatttga acgaaaattt ttattggatt cccaaattgg agtatttaga
    11461 aacgaaaaat tcctattatc tgtatagtgc ttagataata acggatcata ataattattg
    11521 tctttgaact agatatatgt aaatagtatt catcatgatg aaaaatgatg agttctcggt
    11581 gataaaatgt actttttacg gtggaatata catgaaagcg ataagtggat attttataat
    11641 gaagtaagtt agcttatttt gacagaggaa tagtatttta gataacgtat aaaatactat
    11701 ttaatgatgt agaaaggaaa gagagaaaca aaa
//
LOCUS       DAZUJO010000081        11563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000081 DAZUJO010000000
VERSION     DAZUJO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.082"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..3297
                     /gene="fss3"
                     /locus_tag="ACG8OL_002317"
     CDS             70..3297
                     /gene="fss3"
                     /locus_tag="ACG8OL_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS4818285.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            3409..3723
                     /locus_tag="ACG8OL_002318"
     CDS             3409..3723
                     /locus_tag="ACG8OL_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4818286.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3736..4110
                     /locus_tag="ACG8OL_002319"
     CDS             3736..4110
                     /locus_tag="ACG8OL_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS4818287.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            4111..4464
                     /locus_tag="ACG8OL_002320"
     CDS             4111..4464
                     /locus_tag="ACG8OL_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS4818288.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            4533..5549
                     /locus_tag="ACG8OL_002321"
     CDS             4533..5549
                     /locus_tag="ACG8OL_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS4818289.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            6143..8017
                     /gene="ltrA"
                     /locus_tag="ACG8OL_002322"
     CDS             6143..8017
                     /gene="ltrA"
                     /locus_tag="ACG8OL_002322"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4818290.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            8119..8499
                     /locus_tag="ACG8OL_002323"
                     /pseudo
     CDS             8119..8499
                     /locus_tag="ACG8OL_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            8544..9686
                     /gene="dcm"
                     /locus_tag="ACG8OL_002324"
     CDS             8544..9686
                     /gene="dcm"
                     /locus_tag="ACG8OL_002324"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFS4818291.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            9676..9965
                     /locus_tag="ACG8OL_002325"
                     /pseudo
     CDS             9676..9965
                     /locus_tag="ACG8OL_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10221..11405
                     /gene="mobT"
                     /locus_tag="ACG8OL_002326"
     CDS             10221..11405
                     /gene="mobT"
                     /locus_tag="ACG8OL_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS4818292.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            11402..11515
                     /locus_tag="ACG8OL_002327"
     CDS             11402..11515
                     /locus_tag="ACG8OL_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS4818293.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         3876 a         1981 c         2575 g         3131 t
ORIGIN      
        1 actttataaa caaagatgtt caagcgagca tcttattttt ttgtgcctaa tttgaaagga
       61 gaacatttca tgtataaaaa acaaaaaaag aaattatgtg tattaactat tttgagtttg
      121 gctgttcaat caattgtagg agcaattgct cctacgattg cttttgctga tgaaattacc
      181 catccacaaa cggtaaccgt agaactagat ttagcacatc aatatgctgt tgaaggaact
      241 tttagtgatg gtcgtccaat gtcagaagtc actgttccgc attatgcagt ttacaatggt
      301 gtgaagcaag atattttctg tgttgaacca ggagtcccga tttacaatga gttcactcct
      361 ggatatgaga agaacccgtt accagatatg tccgataaag ccaaattagt ttccgtttta
      421 tggaaaaaag taggtacaga tgcagatact catattgtgg cacagaagat gatctggcaa
      481 gaagttaatg ggtataccct tcactcaata aaacgatcag atggtagtgc cgtaaatatc
      541 gcagccattg aggcaaaaat caatcaagcc attgccgatt atcagaagaa accaagcttc
      601 cataatagta cggctaaaac agtattaggg caatcgacta ctgtgacgga tacgaataat
      661 ctgaatttgt cagagttcga tgaggttgtg gaaaataccg caaacattga ttatcgtgtg
      721 aatggcaatc agttgataat cagcccaaat gccaattcaa aagaaagtgg tgtgttgacg
      781 cttaagaaat ctgctggtac cggaacacct gtcgcctata aaaaggctgg ccaacagact
      841 ttaatggccg gggcgatcga taagccgaac acttatacgg tcaaaattga tgtggaaatg
      901 gagggttctt tgaagattaa aaaagttgat aaagaatcgg gtgatattat accaggaacg
      961 gttttccatt tagactttgg aaaaacttta cccgcaaaag acgtgaccac cgataaagaa
     1021 ggcattgcga ccttagatga gattcctcat ggtacaaagg taaccattac tgaaaaatcg
     1081 gtgccggcgc cttatacgat tgataccaca cccatgactg ccacaattaa agcaggtgaa
     1141 accatttcag tgacttcaaa aaatacgcga gaaaaaggtc aaattattct ggataaaacc
     1201 ggaattgaaa cagggagtga tctttggaac gataattact ctttagcggg caatacgttt
     1261 gccattcgca aggatagccc aactggtgaa atcgtccaag aaatgaccac ggatgaaaaa
     1321 ggtcatgcag aaacaccaaa agagattgcc aatgcgttgg aattgggaac ctactatgtg
     1381 actgaaacca aggctagtca tggctttgtg aatactttca aaccagtaaa agtcgaattg
     1441 aattatgtca atcaaacagt ggctcttgtt accagtaacg taaaagggca aaaccaagaa
     1501 attactggtg aaaccacttt gacaaaagaa gacaaagata ccggtcatga aactcaaggg
     1561 aaagctgagt ttaaaagagc tgaatacacc ttgtttactg ttaaggatgg aaaagcggtc
     1621 aaatggaccg agaactttaa accagaattg ttgaagggaa cgaaagcttc tgatgaaacc
     1681 gtaactttga ctttagatga aaagaatcaa gttgccgtta aacacttagc gattaatgag
     1741 tattactggc aagaaaccaa agcgcctgaa ggatatacct tggatgaaac aaagtatcct
     1801 gtatccatca aaaaggtgga tgatagcgaa aaaaatgctg tgattactcg agatgttgcg
     1861 gcaaaagaac aagtgattcg ctttggcttt gatttcttta aatttgctgg atcaactact
     1921 ggcactgccg aaactggctt caatgatcta acctttaaag tgtcgccatt ggaaggaacc
     1981 aatgaaatca ccggtgccga agatgaagcg accacagctt ataacgagca attagggttt
     2041 gatggttatg gcaagtttga aaatcttcct tatggggatt atttacttga agaagtagaa
     2101 gccccagaag gttttcaaaa gattacgcca ttagaaatcc gttctacttt taaagaaaat
     2161 aaagaggatt ataccaagag tgattatatc tttactatta ccgaaaaagg gcaaaagcaa
     2221 ccgattaaaa cggtaacggt cccttatggg aatctaacca atgagaattt ctcagtcagt
     2281 ttgaaccgtt tgatgcttta tgatttgcct gaggaagaag atagcttaac ttctcttgcg
     2341 acttggaaag aaggagataa agaattgact actcttgatt ctaccgagct agtcgataaa
     2401 ttgagctata atctacatga aatcaaagaa gactggtata tcgtagctca agccatcgat
     2461 gtagatgcta caaaagctgc ccaagaaaaa gataaaaaag ccaaaccggt ggcgattgcg
     2521 gaaacaaccg caactttagc gaataaagag aaaacaggaa cttgggagat tctgcataaa
     2581 ttaactgtcg aacaagtttt agacaagacc attgtcttat tcaactatgt gtatgaaagc
     2641 aaagaagcct atgaagctgg tgatgagccg gtagcgaagg atgctagctt gaacaatcaa
     2701 gcgcaaactg ttaattgtat ggtggaacgt catgtttcca tccaaaccaa agcgcactta
     2761 gaagaaggtt ctcagacctt tactcatggt gacgtggtag atatgtttga tgatgtatcc
     2821 attacccatg atgtactaga tggctcaaaa gaagcttttg aaacaattct gtacgcttta
     2881 tttccagatg gtacaagtaa agaaatttgg aaatctggca agattgatta cgaagtgaat
     2941 gacaaagaat ttaccaaaac cgtacttgcg gaaaaagtgg atacaagtaa atatccagaa
     3001 ggtacaagtt ttaccttcaa agaaattaac tatgacaaag atgggaatat caatggaaaa
     3061 cacaatgaag atttgaaaga aaaatctcaa actttaacgc ccaaaaaagt tccgacaaca
     3121 ccaagtacac cagaacaatc tgaaatgcca actgttccaa gtggctctca agaatctagt
     3181 cctacagtga aggcattccc tcaaaccggg gagaaaactt ccaatgtgct actgttcatt
     3241 ggctttagct tgatctttgc gacggctggc tattattttt ggaatcgtcg gaactaaggt
     3301 gataagatga aacatgtaaa tagtagaaaa caatacgcgc gaccgcctcc taaaaaagtg
     3361 gtcgcgctat ttttgtattc aaaactaaca atgaaaagag gaaaaaatat ggaattgaaa
     3421 tttgttgtac cagatatggt cgagacgttc ggaaaactaa catacggtgg tgaaggtgaa
     3481 gttctatcgg aagggtatgg tcgaaatact acaattgttg gtcgaagtta ccatctgttt
     3541 tcaaataaac aacgggccga tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag
     3601 gattttgacc aagatcaacc actaaaagct gtaaatcccc acttaattgc caaaggctat
     3661 gaaattgaga atcgtgggtt taccgattac gtgttgtatg ttgatgattt agtgaaagca
     3721 taggaggaaa agaagatgag attaacagaa ggtattgtcg tagattcagg attaaccttt
     3781 ggcaagttgc gtttttccgc attacgtcga gaagtacgca agcaaaatga ggacggaacg
     3841 gttagtaacg aggtaaaaga acggacctat aatttgaaat cctccgcgca aggtcgaatg
     3901 atccaagtca gtattccagc aaatgtaccc ttacgcgagt ttgcttatga tgcagaggta
     3961 gagctggtca atccaatcgt ggatacggtc gccaattatg tttttcggga aggaaccacc
     4021 gttaattggt ttatcaaggc agatgattta gtcttgaaac gacaactgaa tcaaggaaaa
     4081 cctacgaacc aaaatgaagg aaagaaatag gtgaagaata tggaaattaa tgttgaacat
     4141 attttagatt gtctcgatca atttggcaaa ggtgagctaa cggaggaaca actcactaac
     4201 gcattaacct atgatgaaaa aatgtttctg attatgcatc aagggttact ggaagtaggt
     4261 aacgatgcca aagaagactt tgatgtactg gattggttag gggaagaaga gtctttcttt
     4321 atggttgtcg aggtaaatga aagtttatgt cggcaggcag aatctgtttt ggaagaaatc
     4381 gatgtggaga tgccggatgc catcgaaaga tttttaaaac aattagtgga aacgaaacaa
     4441 cttcccgtag tagtaaatga ctagcataac tttttgaaat tgcccaatga aatcctattg
     4501 ggctttttcg actggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     4561 gagatcaaca cttgatttat cactttgttc ttggctggct aatctttttg ttcattggtt
     4621 ggatgagcgt cttttattta caagaactta gacaatttga tatttctaag gtatcatttt
     4681 ccactagtga aattggctgg tctatgaagg atgtaatctg cttgctagga agtctcgctc
     4741 tttcaggaag catgatgtta ctttatatac acttttttca ggatcattgg cgaagtcttt
     4801 ggcatcgaca aaagttagct cggatgattc tagaaaacca ttggtatgaa gtaaagcaga
     4861 ctcaaggtga aggttttttc aaagatttga atagtagttg gaccaaagag agtatcagtt
     4921 acttccccaa aatttattat cgaatgaaag atggcttact ttctattcgc gttcaaattt
     4981 cactgggaaa atatcaggat cagctattga agttggaaaa taagttagaa agcggactgt
     5041 attgtgagtt agtagaaaaa gaactcaaag actcctatgt ggaatacacc ttgttatacg
     5101 atatgattgc caatcgaatt gggatagaag aaatagtggc agaaaatggg gcgttgcgat
     5161 tgatgaaaaa tcaagtatgg gcgtatgatt ccttgccgca tatgttaatt gctggcggca
     5221 caggtggtgg gaagacctat ttcttactca ccattattca agcactattg aagtcagatg
     5281 cagaattgtt tatcctcgat cccaaaaatg cggacctcgc cgatttaggt acgataatgc
     5341 ctcacgtata ctcccaaaaa gaagaaattt ctgaatgtgt ggaagatttt tacgaacgca
     5401 tgatagctcg tagtaggtca atgaaagaaa tgcccaacta caaaacagga gagaattatg
     5461 cgtatctcgg acttccgccc aactttttaa tctttgatga atacgtggct tatatgggtg
     5521 cgaatcgttt tcctacaagc atcgaataga tgtgaaatgg aagcctattg agattatcaa
     5581 tagtgacaac taatgttttg agagatggaa tgaaagcctt caagttgagc tgaaacatcc
     5641 ccgctaatac tcctacatgc tctaatcaat gaagtagatt ggttttggta tgaagctagg
     5701 tgaagtcgtc tgaaatctgc ctaagtgctt gagcataagg ctgactatag ggcgggcggc
     5761 tgaaaaagat aactatggta agaatgtctg aaaatgactg acgaatctgc gaatgtacgg
     5821 gtctaaaagg ctgatcttat tgaaagatag gaacatacgg acgtatggtg agataccgac
     5881 aagtaaggtt ccatgatatg aaaatcggga tagctaacga agaagctata atttcacagg
     5941 ctcatagtag atacctaagg ttatacatga tagctaaaaa cattggaacg atgtaagcag
     6001 aaaactatca attaaatgac tacttctaga taggtgcata taagtaataa cgaaattgct
     6061 ttgtttctgt gaaagtaagg gcacagtacc gatgaagcgt ctaacaaatg tggagggata
     6121 gccctaagtc tatttaataa caatgaatgt tgaaacagat gaagttcaag gatctggtaa
     6181 gaacgtggga aaatccgtta tggaggtgcc acgcttgaac acaaaaatac gttactggga
     6241 atattatgat ctacaagaaa cattcgatca gctatttact caaagtagaa acgggaaaaa
     6301 gttttatcaa ttatatgagt taatcatttc tgaaaataat attcttttag cataccgtac
     6361 tatcaaggca aacaaggggt cttcgacacc aggaacggat tccttcacaa ttgataacta
     6421 taaggaaatg aaccaagcag agtttatcca tcttattctc agtcatttag aaaattacaa
     6481 accaaaatcc ataaagcgag taatgatacc taaacccaat ggtgagaaac gcccattagg
     6541 aattccatgc atgattgatc gaatgatcca acagatgttc aagcagatac tggagccaat
     6601 atgtgaagct aagttttatg aacatagcta cggtttcaga ccattgagaa gtgcaaagca
     6661 tgctctaggt cgcattatgt atctcataaa catcagtaaa atgcactatg cagtagacat
     6721 tgatatcaaa ggtttctttg ataatgtaaa ccaccgttta ctgataaaac aattatggaa
     6781 tataggaatt tgtgacaaac aagtcttagc tattctttca aaatctctga aatcacccat
     6841 tcaaggagaa ggtatcccga gcaaaggaac gatccaagga ggtatcattt ctccactatt
     6901 atctaatgtt gttctaaacg acttagacca ttgggtctca aagcagtggc atacctttga
     6961 aacaaagtat ccctatacta aaggttacaa taaatttcgt gctctaagag atacaaatct
     7021 aaagcaaggc tatattgtca ggtacgcaga tgactttaaa atcatgacta atgattatcc
     7081 gacagcatta aaatggttcc acgctgtcaa gctttaccta aaagacagac ttaaattaga
     7141 tatttccaat gagaagtcta aaatcgtcaa tttgcgtaaa tgtaagtcgg aatttttagg
     7201 attcgctatt tgcgtgaaac aaaaagggaa aaaatgggtt tgtaattcac gtatttctaa
     7261 caaaaaaaag gatcagatta aagaagagat taaacaaaga atcaaagata ttcaaaagag
     7321 tcctacagca caaaatgctc tcctgtttaa tgctctcgtg ctcggtgttc ataattattt
     7381 caggtatgcc actcatatta atttagattt gaataggatg aactacgttc tttcattcac
     7441 tacttacaac cgattatcaa attgtagtac aagatgttac cccaacaaag cttccccaac
     7501 ttacaaaaag ttttactcaa caacgatgaa aacgtataaa gtagctggag tctatttgta
     7561 ccctctttgc gacgtatcta caaaaacaat ttacggattt aatacggagg atacaccgtt
     7621 tactccgttt ggtcgtcaaa gattggagca caaaagccta cagtctcttg tgttccaaga
     7681 attaagaaaa ttgatggaat cgaaaatacc gaatagaacg gttgagtatt tagatagtag
     7741 aatatctcgt tactcaatga aatcaggcaa gtgtgagatt acaaaacaat ttttgccagc
     7801 aaaagccgtt cactgtcatc actatttgcc aaaaagtctt ggtggagacg acaaattcga
     7861 taacttgaga attattcata aggatatcca tcttctgatc catacaacaa ataaaatgat
     7921 aatcgatcac tacgtgcatg aactgaaact ggtgccagag caaatagcaa aaatcaacct
     7981 ttatcgaaag atgtgtaatc tgcaaaacat tcaatagaaa ataaatagat ggaacgccgt
     8041 atgaggtgaa agtctcacgt acggtgtgga gcgggggaaa agatggagat tatatcaaat
     8101 tcttacctat cgctatagat gttaacgaca aaagaaagtg cggtgatttt gaataagctt
     8161 aaacaaatcg tgatgttagg ccgtcaatct ggtttctttc tgattctagc ttgtcaaagg
     8221 ccggatgcga aatatctagg agatgggatt cgcgatcagt ttaacttccg tgtggctttg
     8281 ggtcgtatga gtgagcttgg gtattccatg atgtttggag aagtcgataa aaattttttt
     8341 atgaaacgca tcaaaggacg aggttatgta gatacagggg gcagtgtgat tagtgagttt
     8401 tatacgccac ttgtaccaaa agggtatgat ttcttaaggg aaattagcaa cattgttgga
     8461 ttaaccgttc atacggaaag agaaaataat agtgtgtagc ctaggaatat caataatcct
     8521 gggctttttt attgaggtga tttatgacat ttttagattt attcgccggg attggtggct
     8581 ttcgcttagg tatggaacaa gccggtcacc agtgtatcgg tttttgtgaa attgatgaat
     8641 ttgctcggag gagttataaa gcaatccatg atacaaggaa ggaggtggaa atgcatgaca
     8701 tcacaagtgt atcagacgaa tttgttcgat ccctcggacc agtggacatc ctttgcggcg
     8761 gctttccgtg ccaagctttt tcaattgcgg gaaagcggca aggattttcc gatactcgag
     8821 gtactttatt ctttgaaatc gctcggttcg ccactcttct ccaacctaag tttctattcc
     8881 ttgagaacgt ccggggattg ctcaatcacg aaggaggggc tacgttcgag acgatcctcc
     8941 gaacgttgga tggattgggg tatgatgtgg aatggcaagt gcttaactca aaggcctacg
     9001 ttccccaaag ccgtgagcgg atcttcctta tcggacattc tcgagatgca tgtaccgaac
     9061 aagtatttcc tatcattgga tcgtcttcaa catctgatca aaacatcaga aacttggtaa
     9121 atatcaatcc ttccaatcga ggaatgggtg gccaagtcta tggttcagat agcgtagcac
     9181 caaccttgac cggtgatgaa ggaattaaaa ttgccttacc ggtaaaggat gggatttctg
     9241 tagcgggtat gttgtcggga aattttgaac aaggcaatag gatttatgag gtaaccggaa
     9301 cagcccccac tttgtcaacg aaacaagggg gaacgaagat tatgattcgg aaaaatgaaa
     9361 cagaatatca agaggtgaag ccaggggaca gtgtgaacct ggcttttcct aattccacaa
     9421 gtcgccgagg tcgtctggga aaacaaagtg tgcatacctt actaacagga gaccaacaag
     9481 cggtagtcac ggatcagtac cagattcgaa agttaacacc tagggagtgc tggcggcttc
     9541 agggctttcc agattgggct tttgatcgag cttctcaagt caattcagat agtcagttgt
     9601 ataaacaggc cggaaactca gtaacagttc cggtcatttt tgatattgct agaagattaa
     9661 agggggtaaa agcatatgat ttatgacgag ttgattggag aaatctactg gatgattggc
     9721 aagattcagg cagatccgaa attggaagaa gaactgtacc ggctcaattt tgatattcgc
     9781 aaaaacgggg tgaaagtcca ggtgattcgt atgtagtgga tgaggaaacg gatgctcgaa
     9841 tggagctcaa tcaagtcatc ggtgaattgg aacgaattgc ggaccttgcc aaagaacctg
     9901 atatccgcca atacttgttt gagataaaag cagagttgga aattgagggt atcaccgcgg
     9961 agtaataggg aaataaccca ctgataggca ctgaccgcgg gggatttgcg aagcagaaat
    10021 cctccgtggt gctgtgcctg ttcatggacg gcggcagccg gccatgatgt ctcacccccc
    10081 gtatctaaca ggggggtaca aaaacacaaa aaacaagcaa gtaaccataa aaaatgcttg
    10141 ttgtatcaag gatattaagt aatttgtata ggtgttaact catttggaaa agttaacacc
    10201 ttgaatttgg aggtgaaaac ttggcgcaac gaaatttaga ttatcggtta ttaaaagaac
    10261 gacgcaacga atatggcgtt tcacaaaata aattagctac tgcttgcgga cttagccgcc
    10321 catacctaaa tcagatagaa aacggtggcg taatcgcctc tacaaagacc atgagaaaaa
    10381 tttttgatca actggaaagt ttcaatcccg atttaccttt aacgttacta tttgactatg
    10441 taaggattcg ctttcccaca acggatgtgc ggaaaattat tcaagaaatt ctccatttga
    10501 aattcgagta tatgcttcat gaagattacg ccttttactc ctatcaagaa caatatgtca
    10561 tgggcgatat tgtagtgatg ttatctcatg aagaagataa aggtgtcctt ttggaattaa
    10621 aaggtcgtgg ttgccggcag tttgaaactt ttttactcgc ccaaaagcgt agctggtacg
    10681 acttttttga agattgtcta aaagccggtg gcgtgatgaa acgcttggat ttagcaatca
    10741 atgatcgagt ggggctactg gatattcctg atttaacgaa aaaatgtcag aaggaagaat
    10801 gtatctcctt gtttcgtacc ttcaaaagtt accgttccgg ggaactgttg aaagctgatg
    10861 aaaaggatgg tatgggcaat accttatata ttggcagcct caaaagcgag gtttattttt
    10921 gtttgtacga aaaagattat gaacaatata tcaagttagg gattccattg gaccagaccg
    10981 aaacgaaaaa tcgttttgag attcggttga aaaatgaccg tgcttattat gcgattcaag
    11041 atttgttaaa aggtcgtagc attgaaagta ccacgttttc cattatcaat cggtatttgc
    11101 gatttgcgga taaagtagat ggaaaacggc gaaccaattg gcccttgaat gaacaatggg
    11161 gtcgttttat tggacggaat cggaaagaga ttcaattaac ttctgaaccg aaaccgtata
    11221 ccattgaacg aacattaaat tggctaggta gacaagtcgc ccctacgtgg aaaatggcta
    11281 aggagttgga tcgattgaat caaacaacgt atatccagga tatggtacag aatgcgcgac
    11341 tctcggaccg gcataaaaag attttggagc aacaaagtat ggcaatcgaa aacttgattg
    11401 tatgaaaaga ggaattttga tggaaattgt aaaagatatg ttattggtac ttggtggcag
    11461 tatgcttggt gttaccgtga tgtgcttgat gaatgcaagt gcagcagcgg attgagcaat
    11521 ggaaaagaaa ggaaagtgac aaaatgaatt tcggacaaaa ttt
//
LOCUS       DAZUJO010000082        11432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000082 DAZUJO010000000
VERSION     DAZUJO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.085"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..290)
                     /locus_tag="ACG8OL_002328"
     CDS             complement(66..290)
                     /locus_tag="ACG8OL_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS4818294.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(488..2068)
                     /locus_tag="ACG8OL_002329"
     CDS             complement(488..2068)
                     /locus_tag="ACG8OL_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS4818295.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2469..3845)
                     /locus_tag="ACG8OL_002330"
     CDS             complement(2469..3845)
                     /locus_tag="ACG8OL_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS4818296.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3973..5061)
                     /locus_tag="ACG8OL_002331"
     CDS             complement(3973..5061)
                     /locus_tag="ACG8OL_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS4818297.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5233..5667
                     /locus_tag="ACG8OL_002332"
     CDS             5233..5667
                     /locus_tag="ACG8OL_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4818298.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5745..6278)
                     /locus_tag="ACG8OL_002333"
     CDS             complement(5745..6278)
                     /locus_tag="ACG8OL_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS4818299.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACG8OL_002334"
     CDS             complement(6372..7550)
                     /gene="mutY"
                     /locus_tag="ACG8OL_002334"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS4818300.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACG8OL_002335"
     CDS             complement(7543..8340)
                     /gene="recX"
                     /locus_tag="ACG8OL_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS4818301.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8447..9829
                     /gene="rlmD"
                     /locus_tag="ACG8OL_002336"
     CDS             8447..9829
                     /gene="rlmD"
                     /locus_tag="ACG8OL_002336"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS4818302.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10003..10581)
                     /locus_tag="ACG8OL_002337"
     CDS             complement(10003..10581)
                     /locus_tag="ACG8OL_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS4818303.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10903..11373
                     /locus_tag="ACG8OL_002338"
     CDS             10903..11373
                     /locus_tag="ACG8OL_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS4818304.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         3364 a         2293 c         1949 g         3826 t
ORIGIN      
        1 aaatttttgt gaattccagc ttatttcttg gagttaaacg cttctgtctc agcctctttt
       61 tctttctagc tttttgattt ttctcttttc ttttgtactt ttctcttttc attttcagtt
      121 ctacgcgtgt ctttattttt aactttttcc tctttatgga tgattgatcg tactttttgt
      181 gcaatttgct cgtttttgtc ccagtctttt acatctaata atcgacataa tcgtaacatc
      241 tcttcttttt tctgtttttt tctttcacgt ctttgccatt ctgtttccat gtgtcacact
      301 ctcctttctt taatcatatg tacgcaaaat aatttttata tttttttgat tagacatttc
      361 tatgatttta atatagtaaa aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa
      421 tatgagaggc tgtttttgtt tggctattct tattacttga tgcagggcag cttgtgtcaa
      481 gacttcttta catcaaactt tttaatttta cattgggata tttatccgca aaccagcgtt
      541 ctgcaaattg gttctcgaat aagaacaacg gttggtcgaa acggtcacga gccaagatat
      601 tgcgacttga actcatcttc tcatccaaat cttctggatc gatccagcga gcgattttgt
      661 gtcccattgg agacatgatt acttcggcgt tatactcatt taacatacga tattggaata
      721 cttcgaattg tagctgacca acagctccaa tgatatattc ttccgttaga tatgttttat
      781 aaagctgaat cgctccttct tgaactaatt gatagattcc tttgtggaag gatttttgtt
      841 tcatgacatt ttttgcagtc actttcataa acagctctgg agtaaatgaa gggagttctt
      901 cataagcaac ttttagtttt ccttcgaaca atgtatcccc aatctgataa ttgcctgtat
      961 catatacacc gatgatatcg cctgctactg ctttttcgac gttttctctc gaatctgcca
     1021 taaattgtgt gacattgctt aatttcagtt ttttatttgt acgttccaac cagacatcca
     1081 ttcctcgttc gaatgttcca gaacagattc ggacaaaagc gattcggtca cgatgggctg
     1141 gattcatatt tgcttggatc ttgaatacga aacctgaaaa ttcttcctca taaggactta
     1201 ccatctgatc atcttgtgtt ttgtgtccat aaggagctgg tgccaattca acgaaagtct
     1261 ctaaaaaagt ctgtacaccg aagttagtca atgcagaacc aaaaaatact ggtgtctgat
     1321 cgccacgagc aattttttct tgattaaatg catctccagc ctctttgatt agttctactt
     1381 cttctaagac ttgttcatat aaggaatttt cagttagagg atgattttcg ggaagcagtc
     1441 catcttcatc caattctact aatcgttcat cctgataatt ttccggacga taaaactcta
     1501 cacgattatg gtagatatca taaagacctt ctagcccttt acccataccg attggccaat
     1561 tcatcggata agattcgata tctaatagct cttctaattc ttctaacaat tccaatggtt
     1621 cacgaccatc acgatctagt ttgttgataa aagtaaatat cgggatgcca cgttttttta
     1681 caacttggaa taatttcttt gtctgtgctt cgatcccttt tgcactatcg attaccatga
     1741 ccgcactatc cacggccatc aaagtacgat aagtatcttc agaaaaatct tcatgtcctg
     1801 gtgtatccaa gatatttact cgtttccctt gataatcaaa ctgcataaca gaactagtaa
     1861 cagaaatccc acgttgtttc tcgatttcca tccagtctga tttggcaaaa ttacctgttt
     1921 ttttgccttt tacggtcccg gcttgacgga tagccccacc aaacaatagt aattgttctg
     1981 taatcgttgt tttcccagca tccggatggg aaatgatcgc aaaagttctg cgattgtcca
     2041 cttgttgctt taattttgga ttattcataa cctaactcca tttcttccat tcaattattt
     2101 atattttctc taggcacaga ttgtccgatc tcttaaagaa agacaatttc atatagggct
     2161 gtgacaaaag ccgtgtagca gtctcttttt ccgagtaaac ggcgttctag agacagccta
     2221 tcagcaataa gcccacccaa gccaaaaata cgaaaagctg tttttgcttg gctgctctta
     2281 ttacttaatg caggatagct ttttcacaac ctttgtcaac tactacaact gaatcagtat
     2341 agcatggatt cagcgaatga aaaagccacc aaaccataag agtcaaatat ttttttgtca
     2401 gctgactaaa agaaaagacg tgttgcttgt ttattcaagc aacacgtctt tcataaccaa
     2461 gtttccaatt atcttcttgg ctcgtcatct tcgaattctt ttctgaagta tcttcgttct
     2521 tcttcaggtt ccgcttctgt ttcttccgga tcatgaaaaa tgacacggat tttaaggatt
     2581 cgagaacctt ccatttcttc agaaaccagc gtaatattat cgacatcaaa cgataacttt
     2641 tcgccttcat ccggaatcat gcctaatgct gtaatcaaat aaccagccat cgtatctaca
     2701 tcactcatat gcagatcact gtcaaaagct tcattgaact catcaatcag catacgtcct
     2761 tggatgatat attcgtgttc atcgattttt tcataaagat tctcaacttc atctgattca
     2821 tcatcgattt ctccgacgat ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg
     2881 ccgccatatt catctaataa aatagccatc tgattctgcg tctttttcat ttcatataac
     2941 agatcatcaa taaagaccgt ttcaggaacg aataaaggct cctgcatgat tttcttgata
     3001 tctaaattat caaagccgaa tttatgggca gcttttagta aattctttgt atgaaggatt
     3061 ccgactactt tatctttgtc ttcgttataa acagggattc ttgaatagtt ttcggataaa
     3121 acttcattca cattttcttc cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt
     3181 gggaccatga cttctcgggc aactttcgta tcaagagaaa aaaccccttg gagcatttct
     3241 aattcttcat tattcaatac gccttcgttt tcaagcatat aacgcatttc atctctggtc
     3301 atttttgaat cttcatcatc aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa
     3361 gcagaaagca gccagacgaa gggcttagct actacaccaa tcaagcgtat cagccctgaa
     3421 gtgaactttg cgacttcttc tgatttattt aaagcaatac gtttagggta caattctcca
     3481 aaaacaatcg atacataggt caaaatagcc agcacaatga tatttgcaat attttttgcc
     3541 gctgtgccgc cacctaatac cggagctaac cgttccgata aagtattagc tagtgaagca
     3601 ccagacaaaa tgttgaccaa agtgattcct acttggattg tcgataggaa attatttgga
     3661 ttctgcaata tttttaaaag cttttgagat ttcacatctc cttcatcagc tttttgctcg
     3721 attcgattct tattgatcga aacaacagca atttcggaag ctgctaaaaa agcattgatc
     3781 aatgtcaaaa cgatcaataa taaaatctgc gctaacagcg actgactctc agggtcagca
     3841 ttcataatcg cattctctcc ttataaaatt aatttttagt ttacgataga aaaaaacgtg
     3901 gcactatagc cacgttaaaa tggtatcaca aagcaatttt ctttgcaata aaaacaagtg
     3961 tactaaaact acctacgagg tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc
     4021 tttgacgatt ttgattatat aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc
     4081 taagaagata ccaagcaatc cggcaatatt tccagctact agaagaacga taatgatcgt
     4141 taatggatgg attttcagtg atttcccaat tacattagga taaatgatat tcccatccaa
     4201 ctgttggacg attagcacaa ccacacaaca aagtaatgct tttaccggat cattgaagac
     4261 tgttactaga aatgcaggtg ctagtcctag ataaggtcct aaatatggaa ttaggtttgt
     4321 caatcccgca atgaccccaa ataagaaagc ataatccaca ccaattacca tatatcctaa
     4381 aaatgtgaag gtaccaacga ataaacattc gattgcttgt ccgctaatgt aattagccaa
     4441 tgttttattc agttgcccca ataactccac aatttggttt cttctttttt ctgggaaaaa
     4501 ctgttggatg tttggcacta gtttttctcc gtctttcaac atatagaaca agatgaatgg
     4561 tgctgtaatg attactacgg ttgccgaagc gatcgttcca acgatcgaac ctaaactatt
     4621 ggatactcca ctaaggaatt gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa
     4681 atatttattg atatctactt ccttgaacaa aggcaattcg gctacctctt ttaaccagct
     4741 ttctacctgt ttgatgaaat caggcatatt agatgccaat gaggaaattt gctgtactag
     4801 acttgggatg acactaagta aaatccaaat aattgctgca actaaaagga gcagcacaat
     4861 tgcgacagcc catattcttt tgatttttgc tttcatcaac agcttgacaa taggattcaa
     4921 taaatagtaa agaaagcctg caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc
     4981 gatcggttcg aaaataaagt ttatttctga tgaaatcatg atcaatgcag ctatgatcaa
     5041 taattcaagc gaccaaaaca ttagtttaga ctgtctaagt ttttcaaaca atagatttcc
     5101 tcctttcttt cttactagta tatattatat tcgttaaaaa ttcttttatt ctttttttat
     5161 ttttgaaaac ttgccatact ttttatttgt tttattttcc agtatactat acgaaaggaa
     5221 ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa tgagcaatat ttacttagat
     5281 gctgtacgga tcaggcatca agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa
     5341 atagacaaag atgaagccca ttgtatccac ttcgtcctgt actcagataa aaaagaaccg
     5401 caaggaactg tacgtctttt gcctttagaa aacggaaaaa tgaaattaca gcggatggcg
     5461 attctttctg aatacagaca ccaagggtta ggaaaaatat tgattgaaga agcagaaaac
     5521 tttgctaaga atcaaggcta taatacgatt ttattagggg cacagtccac agctgaaact
     5581 ttttacgaaa aactaggata tacagcttac ggcgatcctt ttgaagatgc tggcatgcct
     5641 catatttata tgaaaaagac tttataaaaa tttatttcga aaaaagcaaa aaacagcaag
     5701 cttttgagaa aaatctctca ctggcttact gttctttttt gtgtttactt acgcgacaat
     5761 tgcttgtaac ggttatacca aatatcaata tagccttccg agaacgggcc atctccatta
     5821 ttgatccagt caaccagaat cttcacattt tcttttagga tgaagtcaat atcgttcggg
     5881 taattcatca ttttgctgtg catctcatat tcatcgacat ccaatagtcg tttctcgccg
     5941 tcaggaaata ccttgatatc caagtcatag tcgatatatt tcaaggcctc atcgtccagc
     6001 aaaaaaggtg aagctagatt acaataatag gaaaccccct tttctctgat cattgctatt
     6061 acattgaacc agtatttttt atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg
     6121 cgtccatcag attcagtgac taatgtgtga tcattcacgc cgatcataga gtgttcgctt
     6181 gtttttaata ccatggtatc tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt
     6241 tggatcgtca caaactctcc ttcttttgga actcccatag caacccttcc ttaagcagca
     6301 ttttgtgctt tgtgtttggg tgttcattac accgacatca taattattat atcaataaaa
     6361 atgagctttg cctagaaagg attcctattg tttcgatttt tctctaactg atctaccagt
     6421 ttcatttgga ccttaggaaa aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa
     6481 gttttgttct cggtaaattc ttgttctgct ccctcggttg cccgtccgta aaaaaggaga
     6541 acatgccatt tcagatgact aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa
     6601 acaaagagtt cctgcttttc aaatgggaga ttttgctctt cttctgctac taggtcatca
     6661 aacagatcga tttctggctt tgtttcccat tcttttttca atcgctcata ctcctcttgt
     6721 gtcacttcca tcattggaaa tgtccacata ttcgccagca atttttggct atctctttct
     6781 tcaaaataat acgctccaga atgattttga agagctgcac tgatataata aacgtctttt
     6841 ggctttgctt tttttgtttt tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa
     6901 caaaaggctt ggatcggaca agtctcacac tttggagaag ttggtgtaca gatagctgaa
     6961 cctagatcca tcattgcttg gttgaattca cctggatgct tttcatcaat gattttccgc
     7021 atcgcttcat caaagatttt cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac
     7081 aatctgctga ctactcgcat cacgttgccg tcaactgcag gttctggaag tccaaatgcg
     7141 atacttgcaa tcgcgcctgt cgtatatggt cctattccct tcaatgagct aatttcttca
     7201 ggcgtttgcg gcatttcccc atcaaattct gacatgatct gtttagcagc agcttggatg
     7261 ttccgggctc ttgagtaata accaaggccc tcccatgctt ttaaaagctt ttcttctgga
     7321 gcatttgcta gctcttcaat tgtaggaaac cattccatga aacgataaaa ataatcgatc
     7381 actgtgtcta ctctggtttg ctgaagcatg atctcagaga tccagatacg ataaggatca
     7441 cgattatagc gccaaggcag gtttcttttt tcttgttcat accattgaat gaactgatct
     7501 tgaaattctt ttgtttcttt atctgtccat ttttcccatt ctttattcac tttcctcttc
     7561 cttttccgta atgaatcgct ggatcatatc caaatcaaat cctttttgat ataagctttg
     7621 ctttactttt agatttcttt ttttaggttc aaaccttaaa tttcttcgcc atagtttatc
     7681 tccttgtttg actaaataat catattctat ttcttgatct gcttcttgcg gaagatcagc
     7741 tatcgcttgt tggatcactt cttgagaaaa gcctttcgtc atcaatgttt gttggatttt
     7801 tctcaataat tctttttggc tttttccgtg attttttcca aacgcttttt cggctgtctg
     7861 atgtgcattt tgaacttggt cttcaaatgt atattggctg agcgcttgtt caatgatttc
     7921 aggtttcaaa cctttttgct gcatctgctg cgctaatttc tttggtcctt tgtcacttaa
     7981 gcgcatattc gtgcgcacat aactttcccc atagaccaaa tcatcgacaa ggtccaattc
     8041 ttttaagcgg gctacgattt tatttcgatc ttcttgaagg atttcctttt cttttaagta
     8101 tgttcggatt tctttttccg atcgcaactg ataacttaag taattcattg ctaggtgaaa
     8161 accgaaatca gctccgctac tcttcttgat ctcttggata gtttcttcgt ctaattcccg
     8221 tccttttaat aatcgatagc gtactaatac atcttcagat acttgtaaag cttctccttc
     8281 agataaatct acgcgataga aagggccttt gcctttactg attctcgtaa cggttaacat
     8341 aaacatcctc ctcgaacata tattctctat cataccacag aaaatccccg taatcaggta
     8401 ttatatgtta gaataaacca cagaaaaaag aaaaagggag atacttatgg cagaaacaat
     8461 tgtcaaaact ggacaaacgg ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg
     8521 aatcggttat ttcaaacggt tgatcatatt tgttccatat gcactaccaa aagaagaagt
     8581 actagtgaaa atcacgaaag caactcctcg ctatgcagaa ggagaattaa taaaagtcaa
     8641 aaaaacaagc aaagatcgcg tagtggcacc atgtcctgtt tactatgaat gcggtggctg
     8701 tcaattacaa cacttagctt atcaagctca attggatttt aaaaaggatc tgcttttgca
     8761 agcattggaa aaattcaaac cagcaggctt tcgatcttat caacttttgc cgacgatcgg
     8821 gatggatgag ccttggcatt atagaaacaa agcacaattt cagttacgaa aaaataagca
     8881 gactcaaaaa attgaagctg gtttatacga agcaaactct catcagttag tccctataac
     8941 agactgcttg gttcaagaac ctcgaacaca aaaagtaatg aatactgttg ttcaactatt
     9001 gaataaattt gatgcaccca tctatgatga acggacaaac agtggaatct tccgcacaat
     9061 catggttaga atcggtgtta aaactgggga gttacaagtt gtttttatta ctcgcagtaa
     9121 aaaattcccg caaaaaaacg caatgatccg tgagatcaat gaacgactgc ccgaagttgt
     9181 ttcaatcatg caaaatgtcc aggcacaaaa aacgtctctc gtgatggggg atgaaacgat
     9241 ccacttatgg ggaaaagaag ccattgaaga acaaatcaat gaagtgactt tcgatctttc
     9301 tcctcgtgcc ttctttcaac tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat
     9361 caaagcatta gatgtccagc ctaatgaaac gatcgtggat gcttattgcg gagttgggac
     9421 gatcggctta agtgtagcaa aacaagccaa agaagtacgg ggaatggata ccgttcctca
     9481 agcaatcgct gatgcaaaaa aaaatgcgga aagattagga tatcacaaca ctcgatatga
     9541 agtcgggaca gcagagcaag ttctgcctaa atggttgaat gaaggttttc agcctgatgg
     9601 tatcatcgta gatccaccac gtacgggttt ggataacgca ttgatccaat cattattgaa
     9661 ataccctcca aaaaaacttg tttatatctc ctgtaatgtt tctacacttg ccagagatct
     9721 ggttaattta agtaaaaagt tcaagattga atatttacag tccgtcgaca tgtttcctca
     9781 gactgcacgt tgtgaagttg tcgtgaagat gacgagactc gctgactaaa tgaaagaggt
     9841 tgtgaaaaag tctgccctgt atcaagtaat aagaacagcc aaacaaaaat agctcttcat
     9901 gttttatgaa tactgtactt gtgaccgaag agttaacagg aggacactat ttatttaaaa
     9961 aaagggttgt gacatagttt ttgtcacagc cctttttgat tattattgga tattcacgtc
    10021 tatcacttga taaaatgcat ttgtcgtatc gctgatattc catactgcta aaatcacatt
    10081 atatcctttt cgatcctgcg gaagtgtgat cgtttggttg actgtttttg gtggaacagt
    10141 tgctttgtcg tctatttttg tgatcaattc aaaatcacta aatttcaaag gttgattagg
    10201 attccaagtt ggtttggtaa tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa
    10261 attccaagta atatttaatg ttcctggatt aaccatgttt ttatgccatc tgttagatgt
    10321 ctgttcatct aaaggctcaa agccgctgac tcctgcacta gcaatttttc catcaatgaa
    10381 tgtatttttg gtagcttcga tactttgcgg ttcccattgt gctcttccga cattttgatt
    10441 taagtttcct gctgctgatg ttcctaggta tgcacggctt cctggagaag taatataacc
    10501 atgcgcaaaa cctggaatgc taaaaccgaa caaccctaaa ctaactaacc caataaacaa
    10561 taacgtacta atctttttca tgtagaaacc tccttcattt gataaccaca ttgtaaacgc
    10621 tttattgtat taacacaaac attatgcttt tataatttta tgttgaagta aaaaaggtga
    10681 aaaaagtaag atgcagatta taacagaagt agacgagaaa agtacaaata gaaaacatga
    10741 gaatcaaatc atatgatctt tttcaaaaac atcattcata taaacaaatg ttcaactttt
    10801 taataacaac aattatcaat ttttttctta cttcacaaac taaatacagg tcttgctgtg
    10861 agccttcatg gtaaactaat attatagaaa ggagttagta aaatgaagat gataaaagat
    10921 attcgttttt gggtttgtgt tataggaatt gtcatattag gctttttatc agggttgctt
    10981 agcggaaatc ccggaaaata ttactattct ttgcaacttc caccatttgc accacctagc
    11041 tggatatttg ggccgatgtg gaccttactg tatatattga tgggtatttc tctttatttg
    11101 ttacttaatc acaacaacaa aaaacagcga aacaatcttg tcggtctttt tgtgattcag
    11161 ttcattttta attttatatg gtctgctcta ttttttaatt taagaaatat tttcattgca
    11221 gcaatcgata ttacactttt agttattttc ttgtcagtgc ttatgtatca gttgtggctg
    11281 catcatcgcc tggcaatgtg gctcatgatt ccttattatt tatgggtact attcgcaact
    11341 ctattgaatt attcaattta ttttttaaac taagcgctaa aaagagacgt cctgtaattt
    11401 aacacataga aaaagtgcca cctaagttag at
//
LOCUS       DAZUJO010000083        11409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000083 DAZUJO010000000
VERSION     DAZUJO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.086"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACG8OL_002339"
     CDS             complement(69..1490)
                     /gene="tyrP"
                     /locus_tag="ACG8OL_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS4818305.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACG8OL_002340"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="ACG8OL_002340"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS4818306.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACG8OL_002341"
     CDS             complement(3901..5157)
                     /gene="tyrS"
                     /locus_tag="ACG8OL_002341"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS4818307.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5269..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5737..7038
                     /locus_tag="ACG8OL_002342"
     CDS             5737..7038
                     /locus_tag="ACG8OL_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS4818308.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="ACG8OL_002343"
     CDS             complement(7107..7706)
                     /locus_tag="ACG8OL_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4818309.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7961..8842
                     /locus_tag="ACG8OL_002344"
     CDS             7961..8842
                     /locus_tag="ACG8OL_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4818310.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8855..10465
                     /locus_tag="ACG8OL_002345"
     CDS             8855..10465
                     /locus_tag="ACG8OL_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818311.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10542..11201)
                     /locus_tag="ACG8OL_002346"
     CDS             complement(10542..11201)
                     /locus_tag="ACG8OL_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4818312.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT         3324 a         2281 c         2075 g         3729 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     7801 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
     7861 aaaataataa aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc
     7921 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
     7981 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
     8041 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
     8101 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
     8161 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
     8221 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
     8281 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
     8341 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
     8401 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
     8461 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
     8521 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
     8581 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
     8641 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
     8701 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
     8761 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
     8821 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
     8881 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     8941 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     9001 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     9061 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     9121 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     9181 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     9241 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     9301 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     9361 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     9421 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     9481 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     9541 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     9601 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     9661 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     9721 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     9781 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     9841 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     9901 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     9961 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
    10021 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
    10081 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
    10141 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
    10201 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
    10261 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
    10321 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
    10381 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
    10441 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
    10501 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
    10561 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
    10621 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
    10681 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
    10741 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
    10801 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
    10861 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
    10921 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
    10981 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
    11041 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
    11101 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
    11161 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
    11221 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
    11281 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
    11341 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
    11401 ttacttttt
//
LOCUS       DAZUJO010000084        11232 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000084 DAZUJO010000000
VERSION     DAZUJO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.087"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..655
                     /locus_tag="ACG8OL_002347"
     CDS             227..655
                     /locus_tag="ACG8OL_002347"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4818313.1"
                     /translation="MSKVDKNRNKKIQDASRNNNWEEVSRLLDQPLENSLRKDRQYKT
                     VSMNNQVSYNGSLKEYGDNIADTNPDPLEQLIINEEEQHLDHAISRLSKQEQLLILGY
                     YFDSKSYARLAKELDISDKTLKKRLQSTLEKMKTILHENF"
     gene            781..1095
                     /locus_tag="ACG8OL_002348"
     CDS             781..1095
                     /locus_tag="ACG8OL_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818314.1"
                     /translation="MNTRHKKGGYSAQIANDYIDHKQPIHSLSIELEKQFRFEDGKPT
                     KEVTGYKAWFTQEGLPPFVVKFSEAIKLPNYLSLIQFDNLEACEVNYNVYFKASGITE
                     VK"
     gene            1095..2171
                     /locus_tag="ACG8OL_002349"
     CDS             1095..2171
                     /locus_tag="ACG8OL_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002272712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS4818315.1"
                     /translation="MTKKELEQYLLQSLNMALGTLMQGETSYTNSFDCQVTEEGFFFL
                     PRLPAGYIIDDDLYQKIFLIANASLYPRFTLLKQNSAYFVPIDTNDIHIQRALFFPWK
                     KGISERLVIPDLGHFVSSIGDANIPIMKNYSVDYNKVTSILIAGNSGSGKSYALTYFL
                     SILQKFSDLILVDPKFDTPSRWARENSVPVIRPQQNRSKSDFVSEINENLSNCLTIIQ
                     KRQEILYENPSHEFTHLTIVIDEVLALSEGVNKTIKESFFSLLSQISLLGRATKVHLL
                     LVSQRFDHTAIPVSVREQANVLIQIGNINQKTTQFLYPDLDPEGIVIPIGKGTGLIQV
                     IDNEHPYQVLPLLCPTYYTKRGIL"
     gene            2171..2773
                     /locus_tag="ACG8OL_002350"
     CDS             2171..2773
                     /locus_tag="ACG8OL_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818316.1"
                     /translation="MKKSYFMRSLNVTIFLLMIALFTLFLVFLSNFLVVSLSESDTKL
                     IQQTSNVIKTISVSLSKTGLLSATVAVLIIAIEMFNRFKIDKIMNYFKSIYSTMLLRH
                     FLTQTERTEKVMTIETQTITTYNPVNNTFNRAVSKCVVDIRQDFVTIFIKTPSKQQAQ
                     KLLKEMESQIKEEIANRNPDYYFSAPIRTKNALWFEGKRR"
     gene            2961..3563
                     /locus_tag="ACG8OL_002351"
     CDS             2961..3563
                     /locus_tag="ACG8OL_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002941176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4818317.1"
                     /translation="MAKERRSNKWAFLMYEESVPDNYMEILEGLHVPFVLSPWHDKDI
                     HSETGEIKKSHKHGAFYFDSLKSYSQVSELVSDKLNGPAHVEVVMSPKGMFDYFTHAE
                     NPDKTPYYIENIESGAGFELDKFLLEQNTDNFMNDVIDIIEENNFMEFEDIVRYARDN
                     NYLLLGLIIERTYFFAKYLDSRRYSQSKNKAKPTEVKEDG"
     gene            3556..3738
                     /locus_tag="ACG8OL_002352"
     CDS             3556..3738
                     /locus_tag="ACG8OL_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818318.1"
                     /translation="MDKEPLLLNRQQASEYLGIDPKSFDKYIRCHPDFKCFMVGKQER
                     YLKSELLKFLEAHCVN"
     gene            3828..4871
                     /locus_tag="ACG8OL_002353"
     CDS             3828..4871
                     /locus_tag="ACG8OL_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4818319.1"
                     /translation="MATITKRGNSYRATVSLYKKGKYKRETKTFKNKKDAELWTLEME
                     LEKGRGKNIAERSTLFPDFYRNWVETVKKNDVREATYTNYMRTLTVVDSLFQGVQLKH
                     LDDIVMQKKLDHYAETHSKKTTHELVLKIRGSLKYAYARGLITNDFGHLLKARGQEQP
                     KRNIALSITNFKKLRQYCLSHQDEEFNILVLLALETGARRGELLGLTKDDLFEYGINI
                     RRSISPTDNDTQLKTKHSKRDISINKDVYIALQNLAETKLTYLFDWNGFHQSSQLQRL
                     LNKLDIPKTTFHGLRDTHASFLFSKEISIDYISRRLGHNSILTTQNYYLELMPEKKHQ
                     QDADALELLNDLS"
     gene            complement(4999..5616)
                     /locus_tag="ACG8OL_002354"
     CDS             complement(4999..5616)
                     /locus_tag="ACG8OL_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818320.1"
                     /translation="MAKIRYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTRINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(6299..7294)
                     /locus_tag="ACG8OL_002355"
     CDS             complement(6299..7294)
                     /locus_tag="ACG8OL_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4818321.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            complement(7389..8261)
                     /locus_tag="ACG8OL_002356"
     CDS             complement(7389..8261)
                     /locus_tag="ACG8OL_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS4818322.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            complement(8274..9701)
                     /locus_tag="ACG8OL_002357"
     CDS             complement(8274..9701)
                     /locus_tag="ACG8OL_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS4818323.1"
                     /translation="MTKKAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            complement(9755..11029)
                     /locus_tag="ACG8OL_002358"
     CDS             complement(9755..11029)
                     /locus_tag="ACG8OL_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4818324.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
BASE COUNT         3726 a         2140 c         1701 g         3665 t
ORIGIN      
        1 tttaataata taagtataca agataacttc ttgtgcacaa attattaaag tccttatagt
       61 gcgcactaaa cggacggatt gaaacaaata tgatgactag acaacaacag tcttgtcttt
      121 gctaaatatt gtttatccgt ttatttggtg tgcttttttc accaaaggtt caattactag
      181 ctcgtgtatg tttctttctg tccgctaagg cggaaaggac ataatcatgt cgaaagtaga
      241 taaaaataga aataaaaaga tccaagatgc tagtcgtaac aataactggg aagaagtatc
      301 tcgattactt gatcaaccgt tagaaaattc cctacggaaa gatcgtcagt ataaaacagt
      361 cagcatgaat aatcaggtat cctataatgg tagcttgaag gaatacggtg ataatattgc
      421 tgatactaat cctgatccct tggaacaatt gattattaac gaagaagaac agcaccttga
      481 ccatgcaatt tcacgcttat caaaacaaga acaactgctt atcctaggat attattttga
      541 tagcaaatca tatgcacgtc ttgctaaaga attggatatt agtgacaaga ctttgaagaa
      601 acgccttcaa tcaacgttag aaaagatgaa aactatcctt catgaaaatt tttaatatat
      661 tcgtagctag ggactccgag tccctagctt ttttctccct atacatgagg aggtaaaata
      721 ctgaaacaaa aagttcaaat aatttgatga gctaataatt atagaaagag gtacttatcc
      781 atgaatacac ggcataaaaa aggcggctat tcagcccaaa ttgcaaacga ctatattgac
      841 cataagcaac ctattcattc tttaagtata gagctagaaa aacaatttcg ttttgaagac
      901 ggaaagccta caaaagaagt cacaggttac aaggcttggt ttacacaaga aggtcttcca
      961 ccgtttgttg taaaattttc tgaggcaatc aaacttccta actacttatc acttatccaa
     1021 ttcgataatt tggaagcttg cgaagttaac tacaatgtct atttcaaagc aagtggaatt
     1081 acagaggtca aataatgact aaaaaggaat tagaacaata cttgttacaa agcttaaata
     1141 tggcattagg tacactcatg caaggtgaga ctagctacac taatagtttc gactgccaag
     1201 tgacggaaga aggttttttc tttcttcccc gcttgccagc aggctatatc attgatgatg
     1261 atttgtatca aaaaatattt ttgatcgcga atgcttcatt atacccacga tttactttgc
     1321 taaaacaaaa ttcagcctac tttgttccaa tagatactaa cgatattcat atccaacgag
     1381 cgctgttctt tccatggaaa aaaggaattt ccgaacgatt agtcattccc gatttaggac
     1441 acttcgtttc ttctattggt gatgcaaata ttcccattat gaaaaattat tccgttgatt
     1501 acaataaggt cacttcgatt ctgattgcag gaaattcagg ttctggaaaa tcatacgccc
     1561 taacttattt tttaagcata ttacaaaaat tttctgacct tattcttgta gacccaaaat
     1621 tcgatactcc cagtcgttgg gctcgagaaa acagcgttcc tgtaattcgc ccacaacaaa
     1681 atcgctcaaa atccgatttt gtgtcagaaa tcaatgaaaa tttaagcaac tgtttgacta
     1741 ttattcaaaa acgtcaagaa attctttatg aaaatccaag tcatgaattt acacatttaa
     1801 ccattgtcat tgatgaagta ttagctctat ctgagggtgt caacaaaact atcaaagaat
     1861 catttttctc tttgctatct caaatctcct tattaggacg tgccactaaa gttcatcttt
     1921 tactagtaag ccagcgattt gaccatacag cgattccagt ctctgtacgt gaacaagcaa
     1981 atgtgctcat tcaaattgga aatatcaatc aaaagacgac acagtttctc tatcctgatt
     2041 tagaccctga aggaatcgtg attccaattg gaaaaggaac tggcttgatt caggtcattg
     2101 ataacgaaca tccttatcaa gtgttgcctt tgctatgtcc cacttattac acgaaacgag
     2161 gtatcttgta atgaaaaaat cttattttat gcgtagttta aatgtaacta tctttttact
     2221 catgattgcc ctgtttactt tatttttagt ttttttgtct aattttctcg tagtatctct
     2281 ttctgaaagc gacactaaac tcattcaaca aacctcaaat gttataaaga caatcagtgt
     2341 gtctctatca aaaacaggac tgctatctgc cactgtggcg gttttaataa ttgctattga
     2401 aatgttcaat cggtttaaaa tcgataaaat aatgaattat ttcaagtcaa tttactccac
     2461 catgttattg cgtcatttcc taactcaaac agagcgcaca gaaaaagtaa tgactataga
     2521 aactcaaact atcacgacat ataacccagt taacaacacg tttaatcgtg ctgtgagcaa
     2581 atgtgttgtt gatattcgtc aagactttgt aacgatattt attaagacac caagtaagca
     2641 acaagctcaa aaactactaa aagaaatgga gtcacaaata aaagaagaga tagcaaatcg
     2701 aaatccagat tattatttct cagctcctat taggacaaaa aatgctcttt ggttcgaggg
     2761 aaaaagaaga taaaaattgg cgccgtggct cacgaagtga aagccacagg tgccttttac
     2821 tatcattaaa gggattgggg tactacgacc cccaatcccc ccaataatca ataatcaaaa
     2881 aaataacgtt cttccgagtt tatacccaac tttctcctta ttcatgtaga caccaaattc
     2941 tatttgaaag gaagaatact atggcaaaag aacgacgctc aaacaaatgg gctttcctga
     3001 tgtatgaaga aagcgtccct gataattata tggagattct cgagggatta cacgtaccat
     3061 ttgtacttag tccatggcat gacaaagata ttcattctga aacaggcgaa attaaaaaat
     3121 ctcacaagca tggcgccttt tactttgatt cattaaaaag ctactcacaa gtatctgaac
     3181 ttgtaagtga taaactgaat ggtcctgctc acgttgaagt tgtgatgtcg cccaaaggca
     3241 tgtttgatta tttcacacac gcagaaaatc cagataaaac accttattat attgaaaata
     3301 ttgaaagcgg tgctggattc gaactagata aatttctact agaacaaaac accgacaatt
     3361 tcatgaatga cgttattgac attatcgaag aaaataattt tatggagttc gaagacatcg
     3421 tacgttatgc ccgagataac aattaccttc ttcttggtct aattattgaa cgaacctatt
     3481 ttttcgcaaa atacttggac tctcggcgct atagtcaaag caagaacaaa gctaaaccaa
     3541 cggaggtaaa agaagatgga taaagaaccc cttttattaa atagacaaca agcctccgag
     3601 tatcttggca ttgatccaaa atcatttgac aaatatatcc gctgtcatcc tgatttcaaa
     3661 tgtttcatgg ttggtaagca agaacgatat ctaaaatcgg agcttctcaa atttcttgaa
     3721 gctcactgtg taaattaact tggtggttga taaaagaaga ttcgtcattt attctataag
     3781 tgactagtct attttatcaa tcacttttaa taaggaagga tgataaaatg gcaacaatca
     3841 ctaaacgagg caattcatac cgagcaacgg tttctcttta caagaaaggt aaatacaaaa
     3901 gagaaaccaa aacattcaaa aacaaaaaag acgcagaatt atggacattg gaaatggaac
     3961 tcgaaaaagg acgtggtaaa aatatcgctg agcgctcaac actttttcca gatttttacc
     4021 gcaactgggt agaaactgtt aaaaagaatg acgttcgtga agcaacctac actaattata
     4081 tgcggacgct cacagttgtt gatagtttgt ttcaaggagt tcaactcaag catcttgatg
     4141 atattgtcat gcaaaagaag cttgaccact atgctgaaac gcattctaaa aaaacaactc
     4201 atgaacttgt gttaaaaatc cgtggctcgt taaagtacgc atatgcacga ggactgatta
     4261 caaacgattt tggtcatttg ttaaaagcac gaggtcaaga gcaaccaaaa cgtaatattg
     4321 ctttatctat caccaatttt aaaaagctta ggcaatattg cctaagccat caagatgaag
     4381 aattcaatat tttagtatta ctcgctttgg aaacaggtgc tcggcggggt gaacttttag
     4441 gtctgacaaa agatgacctc tttgaatatg gcatcaatat taggcgctcg attagtccca
     4501 ctgataatga tacacaactc aaaaccaagc actctaagcg tgatatctca atcaacaaag
     4561 acgtgtatat agctttacaa aatcttgctg aaacaaaatt aacttatttg tttgattgga
     4621 acggattcca tcaatcttca caactccaac gtctgttaaa taagttggat attccaaaaa
     4681 caacgttcca tggcttgcga gatacacatg cgtcattttt gttctctaaa gaaattagta
     4741 ttgactatat ttctcgtcgc ttaggtcata attcgatact cacgacccag aactattatc
     4801 ttgaattgat gccagaaaaa aagcatcagc aagatgccga tgctttagaa ctgttaaacg
     4861 atttatcata attaacacca actgacacca aaaagtgcca gaaaacgttg atacaataag
     4921 gaattgatta acgttttgag aattgtgaag ctttacgagc tttcttaaga cctggtttct
     4981 tgcaaaatta tattagcctc aactgaaaga tttatttttt tgctccctaa cttcattagt
     5041 aataccatgt ttcattcgat atcttctaaa cgttctacga ttaatccttg ttagctcttc
     5101 aacttcttta tctgtagatc cagtcaaaaa caaattaaat gctaattgaa gtcgcggatc
     5161 attctcacga tattttagcg tacctccctt gtatttccct tttgctttag cgagtgcaat
     5221 tccttgttgt tgcgtttcgc gaatataccg acgttcttct tcggcctggt acttgtatag
     5281 ttcgataatt aagttattaa gtaatcgacg taaattatca tcagtgatac ctgttaacgc
     5341 tggcaaattc aaaacttcca gtgtagcttt cttcagctga attaaattca tcgtttctgt
     5401 tagctcttta ttatttcgtc caagtcgttt taattctgtc acaacgacaa tatcaccttc
     5461 acgtatgaat ttcatcattt cttgaaatcc ttctcgattg gtatctttcc cacttgcttt
     5521 atcactaaaa attttatcac actctgataa taattctaat tgtcggtcta aattttgatc
     5581 taagctgctt actcgagcat acctaatttt cgccataata aaactccttt aaaattctga
     5641 aatccggatc atttttaaat tctgttgccc aactctgttg tcatttttct agataaatgg
     5701 cataaatttt cagcgctaag tgacaaaagc aactaatttt tcatgataga tttttaaata
     5761 tctcaatcta tctccaaaat tagttgcttt tgtagctata gttgactatg aatatgacaa
     5821 ataattagaa aaaactgtgc caattaaata taaaaatgac actcatttac attcaataga
     5881 acaccaagag cccaacaggg tatactctgg tgtcctttta aaaaagtaac tttctgacct
     5941 aattaattga gctactaaaa aagctatgaa atgaggaata aaaaagggat caaaaacttt
     6001 taagtttctg acccttttga gttctaatta tttcttccag aacgaagaat gttatctgct
     6061 ttatctgcaa ttgattctgt tagaaggact gaaaaattta tgccagccct tttgcctagc
     6121 ttatccgccc attgaggaat tgttaaggtt tttctgacaa gttctatgtc tttcaaatat
     6181 ttggcaacat ctacacagat taaaagcatg attttgtatc atagtctatt ataagagatt
     6241 taacaatttt ttatatcacg aattaataat gaaattatca ttgatagtct ttataaattt
     6301 aattgttctt tttctttact gatgtcttcc ccatccataa tgtgacaggt aaaatatatt
     6361 ccttttttgt ttcttcttta tttattcgtt tcatcaaaat agcaacagct gtgtctgcta
     6421 tatcattaat tggttgtatt actgttgtca gatctggtaa tatactccga gtcattaaag
     6481 taccatcata tccgactact aataaatcag ctggtacatt taaccccttt tcttttgcta
     6541 cttgtaatat tagggccgca tcgatgtcat ttgaagcaaa aataccatct attttaggat
     6601 aatcctcaaa catttggaaa aaaatctgtt ttttctttac ataagaaata ttgaaatcta
     6661 atgtcacagt tcgtggaatt aactgatatg ctttcatagt atcttcatag gctagtctcc
     6721 gacgattagc cggagtctgt aagtcaattg gaccgtttgt atgaataata ttttttgcac
     6781 cttgtgcaat taataacttg gttgcaagtt ttccaccatt gtagttatcg gattcaacaa
     6841 ctggaatatc ttcattcatc actcgatcaa ttgcaacaat cggtaaattc tgatatttat
     6901 attcctgaat tccttggtta tgtgtaccaa caattaaacc atctacttga tcagacaata
     6961 actgatttaa ataatttgtt tccttttctt tattattcat cgaattgcct attaatacct
     7021 tataaccaat ttcatataac tttttttcca atgcctcaac gagttcacta aaaaaaggat
     7081 tggctactgt aggaaatagt aacccgacaa tgttagtttt cttttgaaat aattgacgcg
     7141 ctacaacgtt cggttgataa ttgagttctt ctatagcctt ataaacatta tcaatcgttt
     7201 tttgacttaa atagccacga ttgtttaata cacgtgaaac agttgttttt gatacatttg
     7261 ctcttttggc aacatcttct aattttgggc gcattctctt gtctctccta aataatgcaa
     7321 taattaacta tcgaaaaaaa gaactctata gaactactat acctgataat cgatattttt
     7381 tctatgtttt attcttgtaa ttgattcaag cccaaagcaa agtttcctag tgttgccgat
     7441 ccattatttt taattaaagg acgcgtaata tatttactta acggtggtat ttccacgtaa
     7501 tcatccaata atttattaac attaacacga attttttcta acaatttttc atttactacg
     7561 cttccaccaa atactatttt atccggtcgt ataatcaatg ttgtttgaat cacagcttgt
     7621 gctagataat aggccattac atcccaaata ggatccgtta aagaaacgtc ttcacccttt
     7681 tttccaaaac gtgcttcaaa cgttggtccc gcaactaagc cttctaaaca atcaccatga
     7741 taaggacaaa ttcctttaaa atcaatatca tcaggatggc gttttacaaa cgtatggccc
     7801 atttctgaat gaccaaacga tcctaatagt tttccgtcaa caacagctcc accaccaacg
     7861 ccagtaccaa cggtgtaata aactaatgaa tttatttttt cattcgataa agtggacatg
     7921 atgtactctc cataagctga tccgttcaca tccgttgtcc aagacattgg aacattcaac
     7981 tcatcttgta aataacctaa cacattaaca tttcgccatc ccacctttgg tgtagacgta
     8041 atatatcctg attttggtga attttttcga atttctattg gaccaaatga tgcaatacct
     8101 aatgcattta tatcaaactg tttaaaatag tcaacagttt ttttcaatgt ttccctgggg
     8161 gtggtcgttg gacaaattat ttggtcttgt actcggtaat cctcgtctcc cacggcacat
     8221 acaaatttag tcccacctgc ttctatgcta ccaattaaca tcataaaacc tccttactcg
     8281 taagaaattg catctttttt tagatgataa attgtactcg agacaaaagc ttcttcacta
     8341 acactcaaat gcatacttgg ttgattttct gtataataac gctctgtaaa aacaagctcg
     8401 ccatcattaa taaaaatctc tgccgaactt ttatcgataa acactcgaag tgataatcta
     8461 tcacacatct gaatggttcc ataccttgga tcatttttgc cgttacgagt taaggtacat
     8521 tcatttttat ccttagaata aaaaatattg attaactctt catcttttgt ttttaatgaa
     8581 aatttgattt cttgattttt tgatttagtc aatagtacat caattagagc ttctttgaat
     8641 gaagcatctt gagcgattat tatttctcct gccaaattaa ctgtcgtttc aacgccgctg
     8701 ccaactcgaa gttgttctaa ttctctcaca ggtttcatat acaaacgatc tccaattaac
     8761 tctaattctc gaggaagcgt taatgctcct gcccatccgt ctactttttc aggcataggg
     8821 ctttcccaca tatccatcca ggccataaca atacgtctac catctggaga taaagttgtt
     8881 tgtgttgcat agaaatcgtg tcccttatct aattcataaa aatttcctct aatgaatttt
     8941 gctgtctcat aatcataatc accgataaag tagcctgttt catatagatt taaataatct
     9001 tttccatttg cttcaattcc ctgaggggat aataaaagaa tagattttcc atttaattca
     9061 aaaaagtctg ggcattccca cataaatcct tctgtaagta cttccttcgc tttagaaatt
     9121 ggtccaatgt aatcccaatt taataaatct aacgactgat aaacaatagc acgacctaat
     9181 ccatcttttt cttgactacc caaaaccatg taatattttt catcgtgttt ccatactttt
     9241 ggatctctga aatgatgtgt gttatcttct ggtgcttttg ctattacagg atttttttca
     9301 tatttagtaa agtgaatgcc atccgtacta tacgccatgt tttgattttg ccaaaaatga
     9361 tctggatcat tgtctccata atagtgatgt cctgtataga acagatacaa cacaccattt
     9421 ttttcaatag cgcttcctga aaaacaacca ccttcatctt cttgatctcc aggagtcaaa
     9481 gcaagtggta aagattccca atgaactaaa tccttacttc tagcatgtgc ccagtgcatt
     9541 ggtccccatt cagcactata tggatgatgt tgataaaaca catgataata cccatcaaaa
     9601 tagcaaaatc catttggatc attaatccat cctgatggtg cagacacatg atatcccaaa
     9661 cgataacgtg tatttgttaa ttctatagct ttttttgtca taatatattc gctcactttc
     9721 tagataaaat tgttttttat tctgttggtt tccattatcc tcgaataaat ggatcccctt
     9781 gaacatctct atcatcttgt ttaagaataa agaatgcata tatgcctgct agtaatacaa
     9841 taagagaaat aattttaaat gttggttgat agccaacgtt gtctcttaat attcccaatg
     9901 gtgttgataa aatcacttgc ccaatttgag cagctatctg aaatcctatc atatagaggg
     9961 ttgctgatag ctttgtatca aaatgaagtg taaaatagcg aaacattggt aatgtaaata
    10021 atggtacttc taaagcatgc aacattttta tgcatgaaac agataatggt gtcgaggcaa
    10081 acccgcacaa tccaattcgt acacacataa tggttacacc taataacaaa gtatttcgaa
    10141 cccctaattt tctcataatg attggaacaa ttcccatcat taacgcttca caaaatactt
    10201 gaatagcatt caatgtccca tatatttttt caccattagc tgatgttgaa aacaaaccag
    10261 tataaaatcc cggaaacatt tgttgatcga ataccgtata aaatgtccat gtaaaaacaa
    10321 taaagatgat tatcgtccat aattgaggca gtttcattag atcaaacatt tcttttaaag
    10381 aaggaatact atttgattca gcttgttctt gattaaaatt ttgatttgct tcccgttcaa
    10441 ctttagggtt ccaaaataat aaatttaaca atagtaaaga accaaagaaa gatcccgccc
    10501 aaaagtttaa atgaggattt tttacaaata aaaatcctgc caaaagcgct accaaggcat
    10561 aaccaaatga tccccacgcc cttgcttgtc catattcaaa gccaaataaa cgactaaacc
    10621 tttctgacac agcttcaaaa actcctacag aagctaaata tccagctgac aaaaatagcg
    10681 ctcccataat aatgcctaaa ttaaaattgt tatctagcaa tggcccatat atccatataa
    10741 aaaatggtcc aacaagtgtc gctaatacag aagcaaaaat taataaattt cgtttaatat
    10801 acaatttatc ttgtaatgtt ccataaataa acatcaaaat taaggtaact aacgaatttg
    10861 ccgagaatac tgttcctaca gcactgccag ataaccctaa accatttgat tcagatgtta
    10921 gccaaagttg aaaaaatgac caccaaacac cccaagaagc aaaaaatagt aataatgtag
    10981 ctgaactttg taaataagat tgatttttaa atacgttaac ttttgacata tttttatcct
    11041 ccaacgatat atttgtatcc cctttcatga taaccggtta acatttttag aaaaggactt
    11101 gtactcctaa aattataaaa gaaatccgct tacatgtcaa ccggttattc tatttggata
    11161 atgccatata atgtcacagg atatgacttt tattggtgtg tcgcttttct tttttaatgg
    11221 cactattttt ta
//
LOCUS       DAZUJO010000085        11223 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000085 DAZUJO010000000
VERSION     DAZUJO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.088"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8OL_002359"
     tRNA            12..84
                     /locus_tag="ACG8OL_002359"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8OL_002360"
     tRNA            88..160
                     /locus_tag="ACG8OL_002360"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8OL_002361"
     tRNA            168..249
                     /locus_tag="ACG8OL_002361"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8OL_002362"
     tRNA            267..339
                     /locus_tag="ACG8OL_002362"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8OL_002363"
     tRNA            351..422
                     /locus_tag="ACG8OL_002363"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8OL_002364"
     tRNA            439..524
                     /locus_tag="ACG8OL_002364"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8OL_002365"
     tRNA            535..608
                     /locus_tag="ACG8OL_002365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8OL_002366"
     tRNA            618..691
                     /locus_tag="ACG8OL_002366"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8OL_002367"
     tRNA            711..783
                     /locus_tag="ACG8OL_002367"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8OL_002368"
     tRNA            811..884
                     /locus_tag="ACG8OL_002368"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8OL_002369"
     tRNA            898..971
                     /locus_tag="ACG8OL_002369"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8OL_002370"
     tRNA            995..1084
                     /locus_tag="ACG8OL_002370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8OL_002371"
     tRNA            1097..1170
                     /locus_tag="ACG8OL_002371"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8OL_002372"
     tRNA            1189..1264
                     /locus_tag="ACG8OL_002372"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8OL_002373"
     tRNA            1266..1336
                     /locus_tag="ACG8OL_002373"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8OL_002374"
     tRNA            1350..1423
                     /locus_tag="ACG8OL_002374"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8OL_002375"
     tRNA            1448..1535
                     /locus_tag="ACG8OL_002375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8OL_002376"
     tRNA            1557..1628
                     /locus_tag="ACG8OL_002376"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8OL_002377"
     tRNA            1651..1740
                     /locus_tag="ACG8OL_002377"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8OL_002378"
     tRNA            1753..1826
                     /locus_tag="ACG8OL_002378"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8OL_002379"
     tRNA            1845..1917
                     /locus_tag="ACG8OL_002379"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8OL_002380"
     tRNA            1921..1993
                     /locus_tag="ACG8OL_002380"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8OL_002381"
     tRNA            1998..2068
                     /locus_tag="ACG8OL_002381"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8OL_002382"
     tRNA            2081..2154
                     /locus_tag="ACG8OL_002382"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8OL_002383"
     tRNA            2166..2237
                     /locus_tag="ACG8OL_002383"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8OL_002384"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8OL_002384"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS4818325.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8OL_002385"
     CDS             3826..5298
                     /locus_tag="ACG8OL_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS4818326.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8OL_002386"
     CDS             5381..6169
                     /locus_tag="ACG8OL_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4818327.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8OL_002387"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8OL_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS4818328.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8OL_002388"
     CDS             7019..8731
                     /locus_tag="ACG8OL_002388"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818329.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACG8OL_002389"
     CDS             8734..10506
                     /locus_tag="ACG8OL_002389"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818330.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11223)
                     /locus_tag="ACG8OL_002390"
     CDS             complement(10588..>11223)
                     /locus_tag="ACG8OL_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4818331.1"
                     /translation="RKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKF
                     LFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEI
                     IDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHS
                     HISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3225 a         2009 c         2543 g         3446 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtatct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tct
//
LOCUS       DAZUJO010000086        11067 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000086 DAZUJO010000000
VERSION     DAZUJO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.089"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..231)
                     /locus_tag="ACG8OL_002391"
     CDS             complement(58..231)
                     /locus_tag="ACG8OL_002391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818332.1"
                     /translation="MKNSKEEGKNCIFCKKKINSAKKISICSSCRKRSKDSLSALGIV
                     SLSLVGISKKMKK"
     gene            complement(221..1378)
                     /locus_tag="ACG8OL_002392"
     CDS             complement(221..1378)
                     /locus_tag="ACG8OL_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818333.1"
                     /translation="MDEQKETFEISKTVKNNIENFELFNSYVDKYTQDIFEMSSPYEF
                     LLPQYMEKGGTGELSNIFSLDGNSLLNLAGTSINLKELLFSSKNGFVYQPDFDSLSND
                     TKEKIKSGIFKIGESKKIEGNLRAVIVDSTNSNRRVEDLTLKEVVVDKNIARTLTDLA
                     VQAQLKQIYEIVVDIQETQEYQLHWDRNNSILAPFFTARTKILEFKKSGDLNRKIQLL
                     EAASDSMEQAVSAIKADLISNKDHIKKILKKPMYRYSTFERHANFILSDINLLLKIVG
                     IQTYIDLTLDNEEVANDRLEAVKYIFELYSSRNIGATGNIILNTLERLPFINNVIPDV
                     KKVELPSLLEIIHDNYRYTSENKDSWLNINDEMKEGNQFSLLGEGGSEYEE"
     gene            complement(1475..2188)
                     /locus_tag="ACG8OL_002393"
     CDS             complement(1475..2188)
                     /locus_tag="ACG8OL_002393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818334.1"
                     /translation="MRKTVGLFRPLRFDHLDLTAKLLVKYSIPDTFEILDDVLAKEFK
                     GKEGRRKIINNLTRVWSGGKKEPSTFQKEVLAKYNVSDKNDKIIYQYLMTSLAFPFFS
                     ETTTFVGKYLKMSDTFKSSVILSEMRNSYGSAESVYKGVNAVLNGLKSWEILSEGNSK
                     NHYVFKDNLLEISNEFQLNLLVLVVIQNHVGDSLTIEQINNHASMFPFRYLIRKEDIV
                     SSQFEVIEDRIDTYISILS"
     gene            complement(2201..3664)
                     /gene="pglZ"
                     /locus_tag="ACG8OL_002394"
     CDS             complement(2201..3664)
                     /gene="pglZ"
                     /locus_tag="ACG8OL_002394"
                     /inference="COORDINATES: protein motif:HMM:NF033449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system phosphatase PglZ"
                     /protein_id="HFS4818335.1"
                     /translation="MGWEDKIRNMFPRAGEVYLVKDRDNILDDSSVIDMFSRNNIDYI
                     DFLDPIVFRYAYETKYKYERTNTLIIRTDNYSEIPYDVYSNSFQVQIKLKDVITFLDY
                     SAMKEYSIGIPELVHIDSFQNKTLNYEETKRLLSKDQSREDSEEYVLPVANDITTFGL
                     VVMIIGQWRSGDNTLNDKQISSINDVNSKFQNWLIQNFDQTVSLPSYPKPKMVHQIPD
                     FMNHSDYNQKKALIVMDGMSFTQWNIINDYLINNQFKTEVSPTLAWIPSITSVSRQSI
                     FSGKPPLNFSDTINTTNYEEKQWEDFWVDKGFDKSDIFYQRALGSDNYLEMDLQHILR
                     NKKIVGCVIDSIDRFMHAAQQGLWSVHSELNYWLNKGFLLSFLKDLIENDFEVYITSD
                     HGNIESQGVGRINQGVLANSKGERVRVYDDKNIRNRTREDYFEVSENWDSQFLPQNYY
                     PLIAKENYAFITKNDKIVGHGGTSIEEVFVPFVKVTR"
     gene            complement(3676..6486)
                     /locus_tag="ACG8OL_002395"
     CDS             complement(3676..6486)
                     /locus_tag="ACG8OL_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFS4818336.1"
                     /translation="MDQGSLFEEENRPVECLGMTFESDNERREYFRNELRKKLPYLKS
                     IEGFPIGEDEDIIELSDPPYYTACPNPWMNEMIDNWENEKKYENKVVQHAYVSDVSEG
                     KNDAIYTSHTYHTKVPYKAIMRYILHYTNPGDIVLDAYAGTGMAGVAAYYCGKEEEVA
                     NLGILSRKDDIQIDEESFKVGVRHAILNDLSPAASYISNMFNSPRDSSKIYLEIKNTM
                     NDIKSDFFWMYKTITTEDDELKGKMTEELKRINSLDEMKEYMVRYDKSLGDIQYVVWS
                     DVFVCNNCGNELTYFDNGIDWKNHTTLKQFSCPKCDNILNKRSLEHLFEQSYDKFLDK
                     NVSLAKQVPIKINYVYKNKRYNKEIDSFDLNLIEKISRLIEYNNFPIDELPNGYNTKQ
                     PKESHGITHVHQFYTIRNLFIASEFWRRLDWNNRWILTSFISRNATKLNRFVINKYNP
                     NGRINGPLSATLYVPSLSVEQSIFILIEDKLRDIDWNTYGNIVEVSSASDLRINDESI
                     DYIFIDPPFGANIMYSEVNYIWESWIKVKTNNKEEAIQNPTQDKDINSYKNLMIKGLK
                     ECYRVLKSGKWITIEFSNTQAAVWNVIQSVVQEAGFIISSVGVLDKKHGGIRAMTNPT
                     SVKQDLVISAYKPNDAFKNELINNQNSILSIYYFLDYHLEKLPTFIGRNKEAELIVER
                     TPKVLFDRVVAYHIQNGLSVPVSSAEFQEKVAQKYVMRDGMVFLESQVAEYDKKRILA
                     KEFSQQSLFVSDESSAIEWLRQQLMKKPQSRQDLHPQFMKEIQHIAKHEELPELDKLL
                     AENFLLYDSEDVVPDQIAAYLRKNYHDMRGLENQDDKLKSKAKNRWYVPDPNKQADLE
                     KLREKSLLREFNHYVEEVSGTKKKLKLFRSEAIRAGFKQAWSEKDYQKIVDVAEYLPE
                     KIIQEDDKLLMFHTHALMRLDI"
     gene            complement(6494..10216)
                     /locus_tag="ACG8OL_002396"
     CDS             complement(6494..10216)
                     /locus_tag="ACG8OL_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HFS4818337.1"
                     /translation="MKYKDLVNFEPIDSVIQLTDANNKDRALHLLDTFVISDRMADTI
                     NNTIINQLQFSKPVDNKGLMIVGNYGSGKSHLMSVISTIAEYKDSSQHLQNESVAEKA
                     QEIEGKFKVIRFEIGSTEMSLREIITQQLELGLLDLGITFEFPNADQIVSNKNSLLEM
                     MSIFNEKYPDKGLLLVVDELLDYLRGRKEQELTLDLGFLREVGEIAHNTRFRFIAGIQ
                     EMLFDNPRFSFVADSLRRVKERFEQVRIVKEDISYVISERLLKKDEKQKALVREHLTS
                     FTKLYSRLAEDMDKFVDLYPIHPSYLTAFEKVHNIEKRVALKTITMEIQKIIDHDVPV
                     DSTGVVAYDNYWTFIEEDPSNKTIPSVREVLEKTAILKDKVNSSFPSDRKGQMYKDVS
                     LQVINGLALNRLTMDDIYSPVGLTASTLKDDLFLVIPGQLDFLLEDDDPSAFLETSVS
                     VAIKMIMETVSYQYISVNQENGQYYLDLKKDIDIDSLISERTEIIENATLDSYYYKIL
                     QNAVALDDNTYVSGYRIWQHELPWEQRRVMRRGYLFFGSPNERSTAQPERDFYVYMLR
                     PFEKVQYKDELKSDEVFFELSLEDKNFKMNLLNYAAANELYNETTSAQKRLYKDKIDS
                     YFKKLNKWLTERFVDAFKITYKGKTGYLNDFGMFLPQEDNVKSLVNFSAQEFLAEHFS
                     EKYPEYPSFRNYKPGFISSNNIKVVASDALQRINGRNSIQGESVLEGLVLLSNQKTIT
                     SESVTNSAYARWIIETLEIKGNGKVLNNDELFNINAVRGVEDRRLSKSFDLEPELVVV
                     LLGALLSTGKIEVNIDGTTYTAMNFSEFAVLSMEKLTKFSYIKKPSGLPLIEINEILN
                     LFGESAPNNNEEVLEGSIRRMNSKLNTSIEEALKAKRDLSIGYLVGNLDVLDNNTRSE
                     YADQLESFKLFCEQLLRYNSIPKMKNLNVSISEIEKQKQNKELVESLNILKKNVDEVS
                     QLVNYLDQAKYSYGQNSDWSKTIDETVSKLRSNISDGQSYMSNLSELRQLKEEYITQY
                     YTIHEQSRLTATEDNKKQEILRSPQLNMLRRLQSSISLLPGEQLDNWQENLERTKACY
                     QLKKTDLEMSPTCPHCNFLLSIDKRDSRNIVENSMDKLTDIYDNWLNILIDNLKQDSI
                     QDGLTLLTNDEQRSIELLISSKELPLPLNQRFIDMIKNLFDGFEKVELSKEDIIQMLG
                     NGSPMSIDELEGRIRELIATAVDGKDRDKVRIMYKG"
     gene            complement(10228..10686)
                     /gene="brxF"
                     /locus_tag="ACG8OL_002397"
     CDS             complement(10228..10686)
                     /gene="brxF"
                     /locus_tag="ACG8OL_002397"
                     /inference="COORDINATES: protein motif:HMM:NF033453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system P-loop-containing protein BrxF"
                     /protein_id="HFS4818338.1"
                     /translation="MEDIEWLVKMLEEKVEEKKLDYNQFMVLLWNNNTEIKYLFKEID
                     SSILNINRLISEQLLDIPSKLQAIKTQQIITEVIRNNPQKVIVIDNFEILFSPIVKVK
                     PIELFRELSRYKTIIVIWRYSYENNRLVYAVPEHIEYQEAQINKNEMIIN"
     gene            complement(10854..>11067)
                     /locus_tag="ACG8OL_002398"
     CDS             complement(10854..>11067)
                     /locus_tag="ACG8OL_002398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818339.1"
                     /translation="DKIKSISSSIYSYEKYQLEKLEIEISKDNSIISESAQFRYIFGI
                     TLREQVEKLHYDNELLLRSLDNIGIR"
BASE COUNT         3487 a         2017 c         1321 g         4242 t
ORIGIN      
        1 taaaaatcca ttaaattgga taaagttaac accaattaac accataaaat agactagtca
       61 ttttttcatt ttttttgaaa tacctactaa actcaatgat acaattccta aggctgagag
      121 actatctttt gaacgctttc tacagctgct acatatacta attttttttg ctgaattaat
      181 tttcttttta caaaatatac agtttttccc ctcttctttg ctattcttca tactctgacc
      241 ctccctcacc tagtaaactg aattgatttc cctctttcat ttcatcattg atattcagcc
      301 atgaatcttt attttctgaa gtatatcgat aattatcatg aattatttct agtagactgg
      361 gaagttctac ctttttaacg tcaggtatta cattatttat gaacggcaaa cgctccaatg
      421 tattcaaaat aatatttccg gttgcaccaa tattcctact agaatacagc tcaaaaatat
      481 attttacagc ctccagccga tcatttgcga cttcctcatt atcaagagtt aaatcaatat
      541 atgtttgtat gccaactatt tttaacaaaa gattaatatc agataaaata aaattagcat
      601 gcctttcgaa cgtactgtat ctgtacatag gttttttcag tattttttta atgtgatcct
      661 tatttgagat aaggtctgct ttaatggcac ttactgcttg ttccatgcta tcagaagcag
      721 cttctaataa ttgaattttc cgatttaagt caccactctt tttaaactct agtatctttg
      781 tacgagcagt aaaaaatggc gctaaaatac tattgtttct atcccagtga agctgatatt
      841 cttgtgtttc ctgtatatcc actacaattt catatatctg cttaagttgt gcctggacag
      901 ctaaatctgt taaagtacgt gcaatatttt tatctacaac cacttctttt aaagttagat
      961 cttccacacg tcgatttgaa ttagtactat caactattac tgctctcaga tttccctcaa
     1021 ttttctttga ttctccaatt ttaaaaatac cagactttat tttttcttta gtatcattcg
     1081 ataaactatc aaaatctggt tgataaacaa aaccattttt actactgaat agaagctctt
     1141 ttaaattaat agaagttccc gctaagttaa gtaggctatt tccgtctaaa ctaaatatat
     1201 tacttagctc tccggttcct cctttttcca tgtactgtgg gagtaaaaac tcgtaaggac
     1261 tactcatctc gaaaatatct tgagtgtatt tatcaacata tgaattaaat aattcaaagt
     1321 tttcgatatt attctttaca gttttagata tctcaaaagt ttctttctgc tcatccacta
     1381 tttatacaca cccttttagg attctatcac ttcaaactct ggtaattcat caattaattc
     1441 tggagttttc taggcattct ttagattatt actcttatga taatatcgaa atataagtat
     1501 caatcctgtc ttctataact tcaaattgag aagatactat atcttctttt cggattaaat
     1561 atctaaaagg aaacattgat gcatgattgt taatttgttc aatagttaat gaatcaccaa
     1621 cgtgattttg tataactaca agtaccaaga gatttagttg gaactcatta ctaatctcta
     1681 ataagttatc cttaaaaaca taatgattct tactatttcc ttctgataat atctcccaac
     1741 tcttcaaacc atttaaaaca gcattgactc ccttataaac tgactccgca cttccatagg
     1801 aatttctcat ttcagataaa ataacactag atttaaaagt atcagacatt tttaagtatt
     1861 tcccaacaaa tgttgtcgtt tcactaaaga atggaaacgc taaagaagtc atcaaatatt
     1921 gatagataat cttatcattt ttatctgata cattatattt ggctaatact tccttttgaa
     1981 aagtagatgg ttccttttta ccacctgacc atactctagt caaattattt attatctttc
     2041 ggcgaccttc cttaccttta aattctttgg ctaaaacatc atctaatatt tcaaatgtat
     2101 caggtatcga atacttaact aaaagctttg ctgttaagtc tagatgatca aatctcaatg
     2161 gtcgaaataa accgactgtt tttctcattc tatccattcc ttatcttgtt actttaacaa
     2221 atggtacaaa tacttcttca atgcttgtcc caccatgccc aacaatttta tcatttttag
     2281 tgataaatgc ataattttct ttcgcaatta aaggataata attctgcggt aaaaactggc
     2341 tatcccaatt ctcgcttact tcaaaataat cttctctagt tctatttcgt atatttttat
     2401 catcatatac tctgactctt tctccctttg aattcgctaa aacaccttga ttaatccttc
     2461 caacaccttg ggattcgata tttccatgat ctgaagttat atatacttca aaatcattct
     2521 caatcaaatc ttttaaaaat gatagtaaaa agcctttatt caaccaataa tttaattcag
     2581 aatgaaccga ccataaacct tgttgagcag catgcataaa tctgtctata ctatctatca
     2641 cacaaccaac aattttttta tttcttagta tatgttgtaa atccatttcc aaataattat
     2701 cacttcctag agccctttga taaaaaatat cagacttgtc aaacccttta tctacccaga
     2761 aatcctccca ttgtttttct tcataatttg ttgtattaat agtatcgcta aaattcaacg
     2821 gtggttttcc agaaaaaata gattgtcttg atactgaagt tatcgaagga atccatgcta
     2881 aagttggaga tacttctgtt ttaaattgat tatttataag atagtcgttg attatattcc
     2941 actgagtaaa actcattcca tccattacaa ttaatgcctt tttctgatta tagtcactat
     3001 gattcataaa atcaggaatt tgatgaacca tttttggttt aggatacgat ggtaaggaaa
     3061 ctgtttgatc aaaattttga attaaccaat tctgaaattt tgaattaaca tcattaatac
     3121 ttgaaatttg tttgtcattt aaagtattat ctccacttct ccattggcca ataatcataa
     3181 ctactaaccc aaaagttgta atatcattag ctacaggtaa aacatattct tcactgtcct
     3241 cacgtgattg gtctttagat aacaatctct tagtttcctc ataatttaaa gttttatttt
     3301 gaaatgaatc aatgtgaact aactcaggta tcccgatact atactctttc attgctgaat
     3361 aatccaaaaa cgttattaca tcttttaatt taatttgtac ctgaaaagag ttagagtaaa
     3421 catcgtaagg tatttcagaa taattatcag ttcgaataat caatgtatta gttctttcat
     3481 acttatattt agtttcatag gcatatcgaa aaacaattgg atcaagaaaa tcgatatagt
     3541 caatattgtt ccgactaaac atgtctatta cactcgaatc atccaagata ttatcacgat
     3601 ctttcacgag atatacttct cctgctctag gaaacatgtt tcttatctta tcttcccaac
     3661 ccatcataga acctcctaaa tatctaatct cattaatgcg tgagtgtgga acattaataa
     3721 tttatcatct tcttgaataa ttttttctgg taaatattct gctacatcta caattttttg
     3781 ataatctttt tcgctccaag cttgtttaaa tcctgctcga atagcttcag aacgaaagag
     3841 ttttaatttt ttcttggttc cactaacttc ttctacgtaa tgattaaatt ctcttaacaa
     3901 acttttttct cttaattttt ctaaatcggc ttgtttattt ggatcaggaa cataccatct
     3961 atttttagcc ttactcttca gcttatcatc ttgattttct aaaccacgca tgtcatgata
     4021 attttttcgt aaataggcag caatttgatc tggcacaaca tcttcactat catacaacaa
     4081 gaaattttct gccaaaagtt tatctagttc aggcagctcc tcatgttttg caatatgctg
     4141 aatttctttc ataaattgtg gatgtaaatc ttgcctactc tgtggttttt tcattaactg
     4201 ttgacgtaac cattcgattg cactactttc atctgagaca aataatgact gttgcgaaaa
     4261 ttctttcgct aagattctct tcttatcata ctcagctact tgagactcta aaaagaccat
     4321 tccatctctc ataacatatt tttgtgcaac tttttcttga aattcagctg aagagacagg
     4381 tacagacagt ccattttgaa tatgataagc aacaactcta tcaaataata ctttaggtgt
     4441 tcgttctact atcaattcag cttctttatt tcgtccaata aaggttggca atttttctaa
     4501 atgatagtct aaaaaatagt aaattgataa aatagaattc tggttattaa taagttcatt
     4561 tttaaaagca tcatttggtt tatacgccga tatgactaaa tcctgcttta cactagtagg
     4621 atttgtcatc gctcggattc cgccatgctt cttatcaagt acacctacgc tagatataat
     4681 aaatccagct tcttgtacga ctgactgtat cacattccaa acagcggctt gtgtattcga
     4741 aaattcaata gttatccatt taccagactt taaaactcta tagcattctt ttaatccctt
     4801 tatcatcaag tttttatacg agttaatatc tttgtcttga gttgggtttt gaatagcttc
     4861 ttctttattg ttggttttta ctttaatcca tgattcccaa atataattaa cttctgaata
     4921 cattatattt gccccaaaag gaggatctat aaaaatatag tcaattgatt catcattaat
     4981 tctcagatca cttgctgatg aaacctccac aatatttcca taggtattcc aatcaatatc
     5041 tcttaattta tcttctatta aaataaatat tgattgttct accgataaac ttgggacata
     5101 aagtgtagca cttaatggtc cgttaatcct tccattaggg ttatatttat ttatcacaaa
     5161 gcgattcaat tttgtagcat ttctagatat aaacgatgtt agtatccatc gattattcca
     5221 atctaatctc ctccaaaatt cacttgctat aaataaattt cgaattgtat aaaattgatg
     5281 aacatgagta ataccatgcg attcttttgg ttgttttgta ttatatccat taggaagttc
     5341 gtctatagga aaattattat attctattaa tcttgatatt ttttcaatta aattcaaatc
     5401 aaatgaatct atttctttat tataccgctt atttttatat acatagttta tcttaatagg
     5461 gacttgcttt gctaagctta catttttatc caaaaattta tcataacttt gttcaaacaa
     5521 atgttctaaa gaacgtttat ttaaaatatt atcacatttt gggcaactaa attgcttaag
     5581 tgtagtatga tttttccaat caataccatt atcaaaataa gttaactcat taccacaatt
     5641 attgcataca aatacatctg accatacaac atattggatg tcccctaaac ttttatcata
     5701 ccttaccatg tattctttca tttcatctaa agaatttatt cgttttaact cttcagtcat
     5761 ttttcctttt aattcatcat cctcagtagt aatagttttg tacatccaaa aaaaatcact
     5821 ctttatatca ttcattgtgt tttttatttc tagataaatt tttgaggagt ctcttggact
     5881 attaaacatg ttagaaatat atgacgctgc tggagataaa tcatttagta tagcatgtct
     5941 cacaccaact ttaaatgact cttcatctat ttgaatatca tccttcctag ataaaatccc
     6001 tagatttgct acttcttctt cttttccaca ataatatgca gcgacacctg ccatcccagt
     6061 tccagcataa gcatctaaca caatatctcc agggtttgta taatgtagaa tatatctcat
     6121 tattgcttta tacgggactt tcgtatggta ggtatgtgaa gtataaattg catcattttt
     6181 accttctgat acatcggaaa cataggcatg ttgcacaact ttattttcat acttcttctc
     6241 attttcccaa ttatctatca tctcattcat ccacggattc ggacaagctg tataatacgg
     6301 tggatcagat aactcaataa tatcttcatc ttctccaata ggaaatcctt caatactttt
     6361 taagtatggt aatttttttc gcaactcgtt tctgaagtat tcccttctct cattatcgct
     6421 ctcaaatgtc attcctaaac attcaacagg tctgttctct tcttcaaata atgaaccttg
     6481 atccataaaa acatcatccc ttatacatta ttcttacttt atccctatct ttcccatcaa
     6541 cagctgttgc tatcaactca cggattctac cttctagctc atctatactc ataggactac
     6601 cattcccaag catttgtata atgtcttctt tagacagctc cactttttca aatccatcaa
     6661 aaagattctt tatcatatct ataaatcttt ggtttagtgg taatggaagc tccttgctag
     6721 agattaacaa ttctatagat ctttgctcat cattagttag aagagttaat ccatcttgaa
     6781 tagaatcctg ttttaaatta tctattagaa tatttaacca attatcgtaa atgtcagtta
     6841 acttatccat tgaattctca acaatatttc ttgaatcacg tttatctata ctcaataaaa
     6901 aattacaatg tggacaagtc ggactcattt ctaaatccgt tttctttaat tggtaacaag
     6961 cttttgttct ttccagattt tcttgccaat tatctaactg ctctcctggt agtaaagata
     7021 tacttgattg taggcgtcga agcatattta actgtgggga gcgtaatatt tcttgttttt
     7081 tgttatcttc agtcgctgtt aatcttgatt gttcatgaat tgtatagtat tgcgtaatat
     7141 actcttcctt taattgacgt aattcagaaa gattagacat atatgattgt ccatcagaaa
     7201 tatttgaacg tagtttagat acagtttcat caatagtttt agaccaatct gaattttgtc
     7261 cataactata ttttgcttga tcaagataat ttactaactg acttacttca tctacatttt
     7321 ttttcaaaat atttaatgat tcaacaagtt ctttattttg tttttgcttt tctatttcag
     7381 aaatagatac atttaaattt ttcattttag gaatactatt atagcgtagt agttgttcgc
     7441 aaaataattt aaacgactct aattgatctg catattcgct tctcgtattg ttatctaaaa
     7501 catctaaatt accaacaaga tatcctattg atagatcacg tttagccttt aatgcttctt
     7561 caatagaagt attaagctta gaattcattc tgcggattga accttctaat acttcttcat
     7621 tattatttgg agctgattca ccaaataaat ttaaaatttc attgatttct attaaaggta
     7681 gtcctgatgg ctttttaata tatgagaatt tggttaactt ctccatagat aatactgcaa
     7741 attctgaaaa attcattgct gtatatgttg ttccatcaat attgacttca attttccctg
     7801 tacttaacaa cgcacctagt aaaacgacaa ctaattcagg ttccagatca aaggattttg
     7861 atagtctacg atcctcaacg ccacgaacag cattaatatt aaacaattca tcattgttta
     7921 atactttacc atttcctttt atttctaatg tttcaattat ccaacgtgca taagctgaat
     7981 ttgtaacaga ttcactagta attgtctttt gattacttag aagaactaag ccttctaaaa
     8041 ctgactcacc ttgaatactg tttctaccat ttattctttg taatgcgtca gaagcaacaa
     8101 ctttaatatt attactagaa ataaatcctg gtttataatt tctaaaactt gggtattctg
     8161 gatacttctc agaaaaatgt tctgcaagaa attcctgtgc agaaaaattg actaaagact
     8221 ttacattatc ctcttgaggt aagaacattc caaaatcatt taagtatcct gtttttcctt
     8281 tataagttat tttaaatgca tctacaaagc gttctgttaa ccatttgttt aattttttaa
     8341 aatagctatc tattttgtct ttatataatc ttttttgagc tgaagtcgtt tcattatata
     8401 actcgttagc tgcagcataa ttcaataaat tcattttaaa atttttatct tctaaactaa
     8461 gttcaaagaa tacctcatct gatttcaatt catctttata ttgaactttt tcaaacggtc
     8521 tcagcatata gacataaaaa tcacgttctg gttgagctgt agatctttcg ttaggtgacc
     8581 caaagaaaag gtatcctctt ctcattaccc ttctttgttc ccaaggtaac tcatgttgcc
     8641 aaatacgata gcctgaaacg taagtgttat catccaatgc aacagcattt tgtagaattt
     8701 tataatagta actatctaaa gtagcatttt ctatgatttc tgttcgctcg ctaattaaac
     8761 tatcaatatc aatatctttc tttaaatcaa gataatattg tccattctct tggtttactg
     8821 aaatatactg atagcttaca gtttccataa tcatttttat tgccacagac acactggttt
     8881 ctaaaaatgc tgatggatca tcgtcttcca ataaaaaatc taattgtcct ggaattacaa
     8941 gaaacagatc atctttcaaa gttgatgctg ttaaccccac tggactgtat atatcatcca
     9001 ttgttagcct attcaacgcc aacccattaa tgacttgaag tgaaacatct ttatacatct
     9061 gccctttacg atcactagga aaggatgaat tgactttatc ttttaaaata gctgtttttt
     9121 ctaaaacttc tcgaacagat gggatagttt tattagatgg atcctcttca ataaaagtcc
     9181 aataattatc atatgctact actcctgtac tatccactgg tacatcgtga tcaattattt
     9241 tctgaatttc catagtaatg gtttttaatg ctactcgttt ttcaatattg tgaactttct
     9301 caaaagcagt taagtaactt ggatgaattg gataaagatc cacaaactta tccatatctt
     9361 cagctagtct actgtataat ttagtgaaac tagttagatg ctccctaaca agagcttttt
     9421 gcttctcatc tttctttaaa agtctttcag atatgacata tgaaatatct tcttttacaa
     9481 ttcttacttg ttcaaagcgc tcttttacac gtctcaaaga atcagctaca aagctaaatc
     9541 ttgggttatc aaaaagcatt tcttgaatac ccgcaataaa tcgaaatctt gtattgtgcg
     9601 caatttcgcc aacttctcgt aaaaatccta aatctaaagt taactcttgc tcttttcttc
     9661 ctcttaaata atctagtaac tcatcaacta ctagtaataa acctttgtca ggatattttt
     9721 cattaaaaat tgacatcatc tctagtaaag aatttttatt actaacaatc tgatcggcat
     9781 taggaaattc gaaagtaatt cctagatcca ataaaccaag ttctaactgc tgtgttatta
     9841 tttcacgtaa tgacatttct gtactgccta tttcaaatcg aataacctta aatttacctt
     9901 caatttcctg tgctttttcc gcaacagatt cattttgaag gtgctgactt gaatctttat
     9961 actcagcaat tgtagagata acactcatta aatgagattt accacttccg taattaccta
    10021 caatcatcaa tcctttatta tctacgggtt tggaaaattg caactgatta atgatagtat
    10081 tattaattgt atcagccatt ctatcggata taacaaatgt atctaataag tggagagctc
    10141 tatctttatt gtttgcgtct gttagttgga tgactgaatc tatcggttca aaattaacta
    10201 aatctttata tttcattctt cattctccta attgataatc atttcatttt tatttatctg
    10261 tgcttcctga tattcaatat gttctgggac cgcataaact aatctattat tttcataaga
    10321 ataacgccaa ataacaataa ttgttttata acgactcagt tctctaaata attctattgg
    10381 tttaactttt actattggac taaataatat ctcaaaatta tcaataacaa taaccttttg
    10441 aggattattt ctaataactt cagtgataat ttgctgtgtt tttatagctt gtaatttaga
    10501 aggtatatcc aatagttgtt ctgatataag tctattgata ttaagtatag aagaatctat
    10561 ttctttaaaa agatatttaa tctcagtatt attattccat aacaaaacca tgaattgatt
    10621 gtagtctaat ttcttttctt ctactttttc ttcaagcatc tttactaacc attcaatatc
    10681 ttccatccag ccaccaccta taagatttac tttttaaacc acatgtactt attataacat
    10741 ctgaaacgac taatatagtt tgttttttga atgttacctt cttaaagcaa ttcaaatttt
    10801 tctgagtaat aatttagatg aaagcattct aatctatata tccagtataa tttttatcta
    10861 attcctatgt tgtctaatga tcttaataat aattcattat cataatgtaa cttttctact
    10921 tgctctctaa gtgtaattcc aaatatatat ctaaactgtg ctgattctga aatgatacta
    10981 ttatcttttg agatttctat ttcaagtttt tctaactgat acttctcata tgaataaatt
    11041 gaagatgaaa tactttttat tttatct
//
LOCUS       DAZUJO010000087        11053 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000087 DAZUJO010000000
VERSION     DAZUJO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.090"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(367..1158)
                     /gene="msrA"
                     /locus_tag="ACG8OL_002399"
     CDS             complement(367..1158)
                     /gene="msrA"
                     /locus_tag="ACG8OL_002399"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4818340.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1349..1984)
                     /locus_tag="ACG8OL_002400"
     CDS             complement(1349..1984)
                     /locus_tag="ACG8OL_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4818341.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2250..3302)
                     /locus_tag="ACG8OL_002401"
     CDS             complement(2250..3302)
                     /locus_tag="ACG8OL_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4818342.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3315..4253)
                     /locus_tag="ACG8OL_002402"
     CDS             complement(3315..4253)
                     /locus_tag="ACG8OL_002402"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4818343.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4704..5354)
                     /locus_tag="ACG8OL_002403"
     CDS             complement(4704..5354)
                     /locus_tag="ACG8OL_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818344.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5454..5963)
                     /locus_tag="ACG8OL_002404"
     CDS             complement(5454..5963)
                     /locus_tag="ACG8OL_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS4818345.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(6164..7033)
                     /gene="scrK"
                     /locus_tag="ACG8OL_002405"
     CDS             complement(6164..7033)
                     /gene="scrK"
                     /locus_tag="ACG8OL_002405"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4818346.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(7053..8174)
                     /locus_tag="ACG8OL_002406"
     CDS             complement(7053..8174)
                     /locus_tag="ACG8OL_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS4818347.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(8176..8571)
                     /locus_tag="ACG8OL_002407"
     CDS             complement(8176..8571)
                     /locus_tag="ACG8OL_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4818348.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(8590..9387)
                     /locus_tag="ACG8OL_002408"
     CDS             complement(8590..9387)
                     /locus_tag="ACG8OL_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4818349.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(9389..10177)
                     /locus_tag="ACG8OL_002409"
     CDS             complement(9389..10177)
                     /locus_tag="ACG8OL_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4818350.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(10191..10667)
                     /locus_tag="ACG8OL_002410"
     CDS             complement(10191..10667)
                     /locus_tag="ACG8OL_002410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4818351.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(10739..>11053)
                     /locus_tag="ACG8OL_002411"
     CDS             complement(10739..>11053)
                     /locus_tag="ACG8OL_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4818352.1"
                     /translation="QLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCL
                     IERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVK
                     RA"
BASE COUNT         3494 a         2358 c         1622 g         3579 t
ORIGIN      
        1 tatagttatt gtttgcgctt ttatacggaa tttagttcat cattaatgtg gaattcgatt
       61 aggtgtctat taattatcaa gatgattctc tacaaaattc catagaaaaa tcaagattct
      121 acaattcgaa ttctctttca catggaaata aaggctagta aaaaaacagg agttgtgaca
      181 aaagacttgt catacctcct gtttccttgc gacttgaagc tgaatgcttt gcgccacaac
      241 cttttcctcg gtgttctcaa agcaactcct cgaaataatt cataactgtc caaaaatatg
      301 aaaagctatt tttgtcagtt gctcattatt ttgtcggagc tgaacgcttt gcgccacaac
      361 cttcctctat ctatgtcgct gaaaaaataa atgaagctga tgcgaatgct tgatcttttt
      421 atatctttta ggttgcttct gataaaattg ttgatgatga ttttcagctg gccaaaaagt
      481 ctttgcttgg cgaatctctg taacgatagg acggctaaat cggttagatt gaatcagttg
      541 ttctcttgat ttttcagcca attgcctttg cttttcatta acaacaaata gaattgaacg
      601 atattgtttc ccccgatctt gaaattgacc aaaggcatct gtagggtctg aaatttgcca
      661 ataaatctcc aacagctctt cataggttat ttgacgtgta tcaaatatga tctcaacagc
      721 ttcaacatgc ccagtatatc caccactgac ttgatcgtaa tttggttttt tggtctctcc
      781 acctgtataa ccagataaca ctgagattac acctggtttt gtttcaaaag gctcaaccat
      841 acaccagaaa cagccaccgg caaaaatcgc ccgttcttgg tataagggtg aagttatctt
      901 atgtttggca taatcataag cctttgcact cgctgactcc cctgtgattc ttaaataaaa
      961 atccatcgca tctggtgtca aattatctcg tagtgctagc ggacgcaagt tgacctctag
     1021 tttttctatc acctgcttta tcgacatttt tgctaggtct tcttttgcct cttcaaattg
     1081 tgttctttcc cattctcttg tatttcgatt taaaataata ttataaaggt ctatttccat
     1141 ctgctccaca tctaacatga agtcaccctc tttcaagttt tcttcttatt cattcaaaaa
     1201 atgactacct gaaatttagc acaacgcata acaagttaag actaacttgc ttttcgctct
     1261 cctcatctct attcttagtc gaacacatcc acacgaatgg ttacccatag agaaattctt
     1321 ttacttaatc agcttcacac tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa
     1381 tcatcggcaa atagttaaag aaaaaactta agcgttgaaa gaagcccaat tgcttaaaga
     1441 aggcaaactg actaatcgac ggaatccgtg ctaatccgta aagacctgca attagtaaac
     1501 taataactgt taaaacaaga taaaaatggc taagcgtacc ttgaccactt tgacgataaa
     1561 gcagaacaag taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctatat
     1621 tatggatcca agtcgataga ttccaagagc tttcattcgt attgatactg aataacccag
     1681 taaaaataca atcacctatc ccatataaac caactgttgc aacagcaagt attgcaagtg
     1741 tttttgatgt tccaataaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc
     1801 cagatacaac tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca
     1861 cactaattac ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacggtg
     1921 tcgataaatc actaatcaca cctaataata aaaaataaaa accatatttt tgcaaaaaaa
     1981 tcattttctt ctcctacttt ttagtgatta ttcgatacat ccaacaatca aattttttct
     2041 cattttaaca catattttta ttctagttgc cttttgaaca atcaataaca taaaggtgag
     2101 tttcttctac attttaaaaa atcgaatggc actgaagagt tcagttgtca gcatcaatct
     2161 cctactcaaa aaggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc
     2221 attgattgaa aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga
     2281 tcatacagtt taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg
     2341 atgactaacc aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac
     2401 ggaccaacaa gtccgaccac gccgaaacca gcagaagcag gcgtacctga aatagaaaat
     2461 agagcgacag gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt
     2521 tttctaaata gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca
     2581 cctgacttat taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc
     2641 atcgcagccg caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt
     2701 gaaataggag agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc
     2761 ggttgtaagg tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca
     2821 aaagggaata aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac
     2881 acaatcgaca ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat
     2941 gctgtcagca tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca
     3001 cccaaatcag gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc
     3061 atcattctca tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt
     3121 gttgctagtt gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta
     3181 aaatatttta aaatggcacc taatactgca ttaggaatca atccaataat gattcctgtt
     3241 gctgtaccag cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc
     3301 attcaatctc tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc
     3361 aagaaatcac aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca
     3421 ttgatataat cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga
     3481 taatgtaacc caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg
     3541 acatctagtt caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc
     3601 accattttat gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg
     3661 catagtccgt tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg
     3721 tcagaaaaat gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct
     3781 gcttcttttc catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc
     3841 tgaccaggac cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt
     3901 ggtacaaatt tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct
     3961 gtatcttttt tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg
     4021 gtaaatagaa taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt
     4081 ggtagttttg cttctacctc tttaccatta aaatttgctt gtaatccatg ctctttgatt
     4141 tgttggacat gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc
     4201 attaacccaa agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct
     4261 tcctcctttt tctttctatt atattcattt gagaatcatt tcacaacctt gttttgttat
     4321 tcttctctta tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata
     4381 aaaactggaa tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata
     4441 gaaacattca tagaaaaaac ctcactcctc tgattataaa agagggggca ttaacctcgt
     4501 gtatactaaa accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat
     4561 tgtagtacga tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa
     4621 caatacactt atttaaaaac ttctttccca gattttcttc catttttaca gttataacta
     4681 gtattatcat gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact
     4741 tttttattaa ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca
     4801 accggaatta tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat
     4861 atttcattgc catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta
     4921 ttgagttgat aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca
     4981 gtaatgacca ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcatttca
     5041 cctaatgaat caattatttc ttgcccttta gctctaatat attgatctct attacctatc
     5101 tcactcttat ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta
     5161 aattcgcttc tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac
     5221 tctgatttat tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca
     5281 aaattgagcc tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta
     5341 tattcatcat tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac
     5401 aaaaactagc tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact
     5461 tttctccgat tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa
     5521 ggcttaaaat ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga
     5581 tagatttctg cctcagcaaa aacgtctaaa atatcatcag tggtcataga caaggcgatt
     5641 tttaattttt ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg
     5701 tctactatcg gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat
     5761 gcgtcaaaaa cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg
     5821 ttcttatcct gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg
     5881 cctagctcaa aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc
     5941 aataacctat cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg
     6001 tactttttgt cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgataa
     6061 ggcgctgggt atcatgagtg aatgatattg ctgaatacaa atagtccgac cacattaagt
     6121 tgatcggact tcataagcat tttattgact aatacttttt tgcttaagaa ttgattctaa
     6181 catcttttgc taaagttaag catcctatag ttcctgcatt atctgctaac ttaggtgtga
     6241 caatgtattc ttcgatattt ggcgtatcga catagccgtt aattagttct aaaaaacgtc
     6301 tttgtacttt ctttttcaga tgttcttgtt gcatgacacc accaccaaaa attataatat
     6361 ctggtgaaag catcagggtc gtattacagg cacattgtgc aatataatcc gcctcgattt
     6421 cccagaacgg atcttctata gaaagatctt gtcccttggt tcccgtacgc atctcgatag
     6481 cgggtccaga tgccaaccct tcgagacaat catgatgaaa aggacaagta ccagagaaag
     6541 tatctcctga atgtctctta actaacgtat gccccatttc tggatggcta aacccttcaa
     6601 caaaaatccc atcttgtata gctcctccac caattccagt tccaacagtg taataaacaa
     6661 cactggataa accttttcca tgacctgaaa catactcccc gtaacaagca gcattcacat
     6721 cggttgtcca ggcaattgga atttcgaaat gcttattcaa cgttccaaca aaatcaaaat
     6781 ttttccaggc agtttttgga gtagaggtga tatgtccata ggtctttgat tcacgatgga
     6841 tatcaatcgg tccaaatgac cctactccaa tgctttctaa ttgttcttta tagtgattaa
     6901 agaaatcaat taccaaactc attgtttcat tcggtgtagt tgtaggaaaa ctcactcttt
     6961 ctaaaacctt taaatcatca ttgccaatgg cacaaacaaa tttagtccca cctgcttcta
     7021 ttgagccata catttcttac tccaccgtcc ttttatctag gaattcgttc ttccctgtct
     7081 aatagttttt cgaaaaactc atgtggttcc ttttggtacc aataggcaac agaagaaata
     7141 tcgtcttgac gctcataaag cttgatgtca tcatttccta gggtttggaa agtaacacgt
     7201 aaattttctt tgaaactaat ggggtcgggt aaatgccagc ggtacaaccc atgcatcggt
     7261 aaagcatcat cgccaaaacc atgaactgga tttggattag gttttccagc actaaaataa
     7321 tcccgggtgt gatctctttt agattgaaaa ggatagccca taaatagcgt attaaaattt
     7381 tgagcatatg ccctttctcc aaagttcttt ttatggaaag cccaagcgcc gccaaaataa
     7441 tcttctgctc ctgtagatgt tattgtaggg aaatcttcat ctccatctat ataaaactta
     7501 aattctcctt ccccccacca gtatctttcc aatgcgcaca aagcaaagta ggttcctaca
     7561 taatacccag tgcctttgat tccatcaata agggtgtaat cttttccttt tgtcgtgatg
     7621 ggttcacggt tccatttagc atgaaaatac atcgcatcat ccggtacttc agtcaatgca
     7681 taattaaatg tataaaaaat gtgcttaata aactcaggat gttcattcgt taaagtaatc
     7741 ttagcttttt ttcgaaatgg catctcaaaa tagcaattca tccctgatgt tggattcact
     7801 acgattggta aggaattcac ttcgcatctt gtcccaaacc cattacaaaa aaaatctcct
     7861 aatggacttt ctacagaagg agtggattca tcatcccaat aaatacgcaa aattaaatca
     7921 cgtaagacga aactcccagc gattgttttg tctgtcactg tcatccaaaa atgacgtaaa
     7981 attccagggc cttccatttc tgctaaagta attgtctctc cagcatttat cggaacagaa
     8041 ggtgaccctt ttctcccaat tcctagatga ctcgctgtca ttgccgcttt ccctttttca
     8101 ccatttttat tttctggtga aatagttcta ctttcctctc ccttgtggag ccatatatta
     8161 tttaaacaat tcattttatt cgtcctctat cctttcttca cttttttgat tattcatata
     8221 gatgatttgt tgttgtgcct ccgcaataat tgctccaata tctaaattat catttcctaa
     8281 ctgcaaagcg attgacattg ccaagggaag attcattcct gcaattacgt gcactctgtc
     8341 agaaatatag gtaaaaaact tttgatttac acttcctcca gccatgtcag ttaaaacaat
     8401 tatttcgtct gtagtatcca ctaactcttt aatttgttca tctatatcat caatttcttc
     8461 gacatatgca cataagctac taatttgatt atgcatacct gttaaaaact ctagtgtctc
     8521 tttaaaaccc tcagctagta ttgaatggct agcaatgatc aatcttctca taattcaaat
     8581 ccttttcctt tacgctaata ttccaaatgc agccataacc atcgcaaaaa caataacgag
     8641 tagaatcaaa atggtcatac tgattttttt cttactgagt agataatatg ctgcagcagt
     8701 aataattact ggaattaatg ttggtaaaat tttgtccaac attggttgga ttttcatact
     8761 aacttcacca aaagtgaatt taagtggcgt tgaaacactg ataactgaag caattaatga
     8821 tccaactact gttagcccca atactgaagc agcttcagtt aaataattaa tcttttcacc
     8881 gtattcagta acaatcttca ttccctgctt ataaccaaat tcaaagcctc tcattcggaa
     8941 aaagaagatt accatgatta aaatgaacca taaaaagatt cctacaggat tattttctaa
     9001 agccatataa gccgcaattg atcccatgat ggttgggatc atcgtcatca aaagagagtc
     9061 cccaatacca gcaattggac ccatcgtacc aactttaaag tcattaatcg catcttttgc
     9121 ttctaatcct tgcttttcct ccattgcaac acaagcacct aaaataatgg ctgccagcca
     9181 aggctgtgta ttgtaatact gaaagtgatt atttaaagct tcgatgtatt catcatcatc
     9241 tttgtataat tttcttaaaa taggtgacaa tgcgtaagtc acagatgcac cttgctgtgt
     9301 ttgataatta aaggtacaaa ctgacatcaa ccaacgatag ttcgctttcc taagatcctt
     9361 tttcgttact tgatattttt cgctcatttt attcatcctc cccaataaac ccaacatcag
     9421 aagctcccgc agaagcagtg atttgtgcat ctttttcagc tctatgatac acattaaatg
     9481 ctaaagcgaa tcctatgatc gccgctccaa taataggtac cttcaaaaaa gcggctaaga
     9541 aaaatccaac aagtaaataa ctaatgtatc gacctgtagg cataaagcgt aacaacatcg
     9601 caataccaac agcaggcaac atttttccgg ctaatgttag cccatccata aaccaattag
     9661 gtaaaacatc caaaacgtaa ttaacagcag actgtcctaa agtaacggca ataaatactg
     9721 gaattgctgc agataaacca aagaaaattg gccccaacca caaaacacta ttcattttct
     9781 taaattctcg tttattcgca tattcctgtg atttgcgaac aacaaaacca tttaagattt
     9841 tgacaattac atctagttgc acccctaaca tacctacagg tagtcctatt gctaatccgg
     9901 cttctactcc aagctggcta ccaaatgccg ttgcaataat tgcgcacgtt ccatagtccg
     9961 gtacagatga gccaccaaaa ccggcaactc ctaatgacat caattgaact gtaccgccaa
    10021 ctatcaaacc tgtttttata tcacccatca aaattcctgt gaataatccc caaaaaactg
    10081 cgtaatttac aaagatcatc ggaatcccat aataatcaac atgtttaata aatgctaata
    10141 tagtcagcat aataatttga aaaatagtaa aatgcattat ccttactcct ttatcctaat
    10201 tttgattgaa tttcctgtgc cttatccgcc ggaacaaatt gtgtaacaat ttctacacca
    10261 gaatccaaca attttttata cgtgctgata ttttcatctg atgcataggc tctagaagat
    10321 agctgtaatg cattatcttt aacaacagta ccgccaacaa ttagtcggtc aattctaata
    10381 ccgttatcta ctaaatgaag gattgtttcc ggctctttca cgatgacaaa tactttttcc
    10441 tgatcgtatt tccccttttt aaaattcgca atcgctgttt cttcagtgat aattgataca
    10501 gccattccag caggtctagc taaacgcata cttgattttt ggatgtcgtt atttgcgaca
    10561 gaatcatcga tcaccatcac tctgtttgct ttagacattg gtgcccattg cgtgacaata
    10621 atcccatgca ataaacggtt atcaactctc gacataacaa tactcataaa tacttcctcc
    10681 tttatctttc attttcttac aagcaattac catgccaaaa caaaaagaac ttgataaatc
    10741 aagctctttt tacataatca taaacgtatt taacctcatg aatcgggatg tcgacactat
    10801 aataatgttc aacgacactg aaagattgct taaaatgtgt ctgaaatttt tgaaattcct
    10861 cactcgtttc attgaaataa atttcatctt ccaaagtatt ttgttttact aatcgttcaa
    10921 ttaagcaact aatgtgaaca tataatccga aacacgtatt attatcaaat gaaattccta
    10981 gatcattttg taagatgtat atagcatctg aaacccgttc taataattta gtcggattta
    11041 atatggtcaa ttg
//
LOCUS       DAZUJO010000088        10774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000088 DAZUJO010000000
VERSION     DAZUJO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.091"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..1181
                     /locus_tag="ACG8OL_002412"
     CDS             258..1181
                     /locus_tag="ACG8OL_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818353.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLENINETVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            1364..4039
                     /locus_tag="ACG8OL_002413"
     CDS             1364..4039
                     /locus_tag="ACG8OL_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS4818354.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKIWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            4221..4460
                     /locus_tag="ACG8OL_002414"
     CDS             4221..4460
                     /locus_tag="ACG8OL_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818355.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            4476..5849
                     /locus_tag="ACG8OL_002415"
     CDS             4476..5849
                     /locus_tag="ACG8OL_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818356.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            5853..7490
                     /locus_tag="ACG8OL_002416"
     CDS             5853..7490
                     /locus_tag="ACG8OL_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818357.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7653..8585
                     /locus_tag="ACG8OL_002417"
     CDS             7653..8585
                     /locus_tag="ACG8OL_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818358.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            8701..9021
                     /locus_tag="ACG8OL_002418"
                     /pseudo
     CDS             8701..9021
                     /locus_tag="ACG8OL_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            9199..9558
                     /locus_tag="ACG8OL_002419"
                     /pseudo
     CDS             9199..9558
                     /locus_tag="ACG8OL_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            9622..>10774
                     /locus_tag="ACG8OL_002420"
     CDS             9622..>10774
                     /locus_tag="ACG8OL_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4818359.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVF"
BASE COUNT         3871 a         1594 c         2102 g         3207 t
ORIGIN      
        1 acggtaagga ggtgaaaaca ttaagaatta agggctttta gtttattctt tctctccctg
       61 tctaccggtg aaaacattta atataaaaaa aggataaagt cgataaaggc ggactcaatc
      121 gatattaccg gtagagagac cagttttttt taagaataaa gtgtgaattg acgtatcttt
      181 tattcttttt tctagtctac cggtaaattt tgagaggagc gtcatatgaa atttattatg
      241 aataaggaga tgtattaatg gctacaaatt acgcaaaaaa gataaatttt gatgtggata
      301 aactttataa aagtttgata gttgttccca aaaataaatc agaagatgga aattatgaat
      361 tagaagtttt gcaatttaag attgaacaaa agtccagtga aagatttatg agcaatcaat
      421 tagtgaaaat caaaaaatgg ctccctatga ttgatcaatt agtatctaat aataaggaag
      481 ctaaaaattt atgtattttt gtcttattat tacatggaag agttattaac ttagttaatc
      541 gaaaaagtac gactaaattt gcaaactcat tctggttttt agaaaatatt aatgaaacag
      601 tggaaaagaa tggagtttat aaaggtgatt ttcattttca aaatgctgag actattgaac
      661 gagaacatca ttatttcgat ttattagtga atacagtggt agaattagcg aaatgggcaa
      721 agtcaaacga aacttatagt caagtactta aaagaattat gagtcaagaa acttcattta
      781 tctatgagtt aagcaagtca aatttgaaat atacttataa tgaagatgaa cttgaaattt
      841 tagataatcc agaaaattca caatggatta aagagttgaa gagagaagac tttattgaga
      901 cactaagaat tctaaaatcg ttatttgatt tgaaattagc caaagctacc atcatgggaa
      961 ttgaaagaga ttcatattat tgggaggaaa ttggactata tgctccccag attgaaggtg
     1021 aagataaaga gaaaaggata gacaacatta ttaactatat taatagttta agtgaacctt
     1081 taaaatgtag aggacttgat tttataggaa cattaggatg gacagaaaca tactatgaga
     1141 aaatatataa agaaataatt gaacaatatt ttcctaaata atttaataag gattgattta
     1201 ttgtaaaaga aaaaagttgt gattttggaa atcacaactt ttttcttttg tcaagatttt
     1261 gagtgggata tatttttgta gaactgaaga tcatgaaatt atgagaggtt ggaggtaatg
     1321 tagtcataca aataaaaatt ttttagaaag tgagtggtaa ttaatgaaaa tgaatcacac
     1381 ttcaattcag aagcatttac aactgttaaa tacactgggg atttcttatg ttaatttgag
     1441 aactttttct acattaaaca agaaaaccgt ttctcatatt ttttctactg cagattctca
     1501 agcaattatt aactatttag aaaaacagaa attgtctgaa ggtacagtaa acataactta
     1561 taatcctatt aaacaagagg tgattacaca aaaaaagcat tctgtgagag acaatgatat
     1621 tgaaaaaatt cggtttgtat ttattgatat tgaccctaaa aggaaagagg gaagtgcaac
     1681 agatagcgaa aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt atttgaaaga
     1741 aaagagaata gaatcatttg taaaagttga tagtgggaat ggctaccata tttttcttcc
     1801 cataaatgaa caaccaaata atcacgagac gattcttact attcagaatt ttcttcaatt
     1861 acttcaccga agatttggag tagaagatga agttgatatt gatacttctg tttataatcc
     1921 ttcaagatta tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca caaaagagcg
     1981 tccgcatcgt ttatctagtt tcctttctat tcccgataac atagatagaa gtaattgttt
     2041 taaaattctt gaacagatag ttgaagaaaa ttccatgatg tcaacctcta gagtaaaaag
     2101 ccatccatct tttagagaag tgttaccttt tattcaagca gactataaac aatggttagc
     2161 tcactatcct gaattaactt ataccataaa agagggcgat cataaaggag ttaccttact
     2221 tatatttgac gaatgtccat taagaaaaca cacgaataat tctaatggtt cttgtttgtc
     2281 tgttacggct gataataaga ttcgatttca ttgtctgcat gcttcggata aaaataaaga
     2341 tattcaagac tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt ctgtagaatc
     2401 attagaatcc aatcaagaat attattttga ccagtttaaa ctgaatcaag aaggagtatt
     2461 tttggtgaaa gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta agcaaatacg
     2521 caaaaatatc gaacaaaaag tcattacact tgttcttgcc tattatgtcg atcattgttg
     2581 ggatgaaata gaaatagaag gtggatgttt acaaactaat aattttaaac aattagcaaa
     2641 acatggctta atcattaaag ctagaagaga agctgaagtc gttgactttt tacagattca
     2701 acgggagaat gctccaatct tttttgaaca tgaatctctt ggctggaaaa aagaaaatga
     2761 agcaatgatt tttcagttac aagattcttt tggacgtgaa attccttctg tattaactaa
     2821 aaactcatgc taccaattaa caactaatgg tacagaagcg gaatttgacg cattggttca
     2881 taagtatgtt ttaggaacga acttagaact tgcttggtcg attggtattg cttctattgt
     2941 gttaggctat ttaaacgtta cgaaggctaa tacttttttg agtttactaa ttaacctaca
     3001 gggcaagagc acaacgggta aaaccactac gttacacttt attgcctctt tatttggaaa
     3061 tccaactacg gttcttagaa atatgaatgc taccaataaa gccattataa agctagcatc
     3121 aaacaatcag ggaggtgttc cactaatact agatgagtta ggagcagcaa ctcaaaataa
     3181 ctttagctcg ttgatctatc aattagcttc aggagaagaa aggctaagat tagattctaa
     3241 ttgtcagctt atgccacaag agactttttc tgttttgacc cttacctctt ctgaagagcg
     3301 tttggaaaat tatttcaatg agaaattaac tggtttgaaa gttcgatata tcgaattttc
     3361 tgatatcaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc tttcttctaa
     3421 tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag ggattgcaaa
     3481 aattgaagca acttttgtcg aagctcaaaa aaaaatatgg gctgttttac ctgaacattc
     3541 tttgaagcaa cgcattggta ataatctagc tttgatttat agtggagctc tcctaattca
     3601 agatatactg ggctatcctt tacgattaga gcatatacag acggtgctta ttaattcgta
     3661 cgaacagtta attgtagata ctagtttagg aagtgagcaa acggtatttg accgagtgaa
     3721 aacgttgttg gtgatgaata gccataaatt tttaacaaac agtgacaaca aacatacgcc
     3781 cagtacgatt tggggaaaag tatcaattca aaaaaatggg gatattaagg taaatatttt
     3841 tcctatcccg tttgaggact tgttaaggcg agaatttcaa gtaaaggatc taacctatct
     3901 ttttagacaa ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa gaacgaaacg
     3961 tattcacatg aatcgtcaag ttcttccaac atacgagatt ctattacctt cttcactcaa
     4021 atcgtatttt cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc accatgttat
     4081 ggaaatattt cttattgatt tagcaaaaca actgattact tatctgtatg atgacgagca
     4141 aacccaaacg gaagtaaatg aagcttgatc tatgcttcat ttacttcatt caatcaaaca
     4201 tttaagaaag gggaattcta atgactaaaa ataaaatgac actaaaagca gaggttcttc
     4261 tgtatattca agaacatttt tctaatcaag ccttttttac aaaaccaatt tacttggctt
     4321 tcgaaataag aggggtatct gctggatcta ttggaggtac tcttcaagcg ttgaagaacg
     4381 aaggatattt agaaaatcac tttgtgcaac gttcgttcaa tggaagagtc gcgaaagaat
     4441 ggtatttagt tcattcatag agaggtgatt caagcatgcg tgtggcttgt tatgatcgaa
     4501 cgtctgttta tgaagaagcg aaaatcagta atgacgctca atatgatgca gtgaaagagt
     4561 atgttttaga gaaatatgaa caaattcctc aacgattttc agacaaagga gaatcaggtg
     4621 gaaaagcgga tcgtccagct tttcgacagc ttagagcttg ggtttgtgcg tcagaagaag
     4681 agggggtgat ctatgtctgg cgttatgatc gattggcacg tcaagccaaa atagccttag
     4741 aatttattga gttgtgccaa caacatcagg ttcaagttta ttcgatttct gaacctcttc
     4801 ctgcttcttt ctcgcacgcg tcaacgaata aactatttgt gcaattattg ttcacttttg
     4861 cagaacaaca acgaaacgtc attattgaaa atgttcgtgc cggacttgcc tataaaaagc
     4921 aacaacggca atatttgtct tctgcggttc catatgggta tcgtttagtg aatgggaaaa
     4981 tccaacaaga agcacatgaa gcaaaagttg tccgccatat ctttcaactt tatttgacta
     5041 aaaaatatgg ctacaaaaag ctttgtcaac gattgacgca acaaaagttc ttcttccgtg
     5101 aacgtccttt tcagccctat catatttata gtatcctaaa aaatccattg tactatggcg
     5161 aaatcaaagg aggatctttg gggaagtatc taggaacctt tgaaccgatt ctctcgaaaa
     5221 caatcttttt acaagcccaa gagattcgcc aatcaaggtg tacagctaaa aaagatacgt
     5281 atccgtactt attacgtcaa aaaattcggt gtcccttctg tggtcgtcat ctgagttcga
     5341 agtatcaatg gaatacaaag aagacgaaga cgcttcatta ttatcattgt accgaccgaa
     5401 gctgccaagg aatctttctt cgggcagaaa gggttgagtc agaagtattg gaaaagatac
     5461 aggctttttt tcaatacgaa ggcgtctatc aagccttgat tgcccaattg acgcaacaat
     5521 tagccgaaaa gcgaaaagat gaaaaacaca aagtaatcca acaaaagaaa caagaagcac
     5581 aatggctcaa ggcatttgaa gagggcaatt gttcactgga agaactcaag caacagctgc
     5641 aagctcttca aaagccgagg tctacttctt cgtttctttc actccaacag tgtgaagcaa
     5701 aagtgaaaga atggcttcaa ctaaaagacc tctctagtcg acaattgtta tgtgaccatg
     5761 tttgtcggat cgaggtaaca aaggaaaaaa cagtctgtgg gatttacttc cgacaaatga
     5821 cacaaaatat tttagaaggg aacgaatgat ctatggaaac gacaccaacc aaacgagtcg
     5881 cattgtatat tcgggtgagt acccaagaac aagctttaga aggacatagt attcaagcac
     5941 aagaacaaaa tggacgacag tttgccgaac ggatgagcta tgaagtggta gaagtttatg
     6001 ccgatgaagg gattagtggg aaatccacaa aaaatcggtt cgcctatcaa cgaatgatga
     6061 acgatgctcg gaaccatcgt tttgatctac tagtgatttg gaaattgacc cgtctcggac
     6121 gcaatatgtt agatgtctta caaaccgtcg aggaactgtt gcgatatgat gtggggttac
     6181 attcgatctc ggaacaattt gacgtgacta cttcgacagg aaaactaatg ttacaactcc
     6241 ttgggagttt cggtgaattt gaacgcaacc aaattgcgga gaatgttcag atgtcaatgc
     6301 aaagcttagt gaaagataaa aagcgttatg caggtggacg acgtttaggc tatgtcagtg
     6361 gtattgacac gaatggtcaa aaacaattgg tcattgaacc agaagaggcg aaaattgttc
     6421 gtctgatcta tgcaaaatat ttaggcggag cagggtatcg agcgattgcc aattttttga
     6481 atcgtcaagg atatcaaaca gtcaaaaaga atgctttttc taccacggca gtcaaagata
     6541 tcttacgcaa taaaatttat gcagggtatt tagaatatgc acgctatatc aattgggaaa
     6601 ccaaacgacg aaagggcaag aacccaaaac cgattttagt gaaaggtacc catgaaccga
     6661 ttatttcgga agaagattat caaaaagtgc aagaacgcct tgttttagaa ggcaaacagc
     6721 ccaaatggaa ccatacaggt gaaaatgtat tgacaggctt gttacgttgt ccagaatgtg
     6781 gggcaccgat ggcagcgagt aatgtcacaa atactctaaa ggatggcaca aagaaacgaa
     6841 ttcggtatta ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt catgccaatt
     6901 cgattcgtgc cgaacaagcg gaaacatttg tcgccgaccg attaaaggag atcgttcagc
     6961 taccagaagt tcttccccga ttggtggcag cgttaaatga agaaatagtg cgacaaagcc
     7021 aaccattgga acaagaactc gtggtgttac tggaacgaaa ggaagaattg aagactaaaa
     7081 ttgaaaaatg ggaagctgct ttggaagata gtcctgaatt gtttccgatg ttaaaagacc
     7141 gcttagacga attgactgaa aaacgacgac agttgcacat tcgagaaaat gaaatcttag
     7201 ggatctttca acaacaagga gagccgattc aagtcaaaga tgtccaacgg atattgacta
     7261 gtttggatcg ttttcttgcc cagagtgaga agaagcaagt caaagccttg tatcgtacct
     7321 ttattgagaa aataaccttt gatcctaact ataaggagat attggagatt acaatgaaat
     7381 tcacgcctgc cgtggtacaa caattgaatg agcaatatca aatagccgta tccaaaacaa
     7441 aggatacggc tatttttgtg ttaaagacac cctttacgtt aacgatctag tctaggttat
     7501 atcaaacaac gttagagggg cgctaacgct tctctagcga tttgaaatcg ttagatgagt
     7561 cgttagggag gtccttatca taaataggga tatttattaa tgaatatttg aaatataatt
     7621 gtatataaga attaaaaaga gtggggatag atatgaaaaa gatgggtaag tgtattttat
     7681 gtgaaaaata cactgaactt actaaagaac atgtaccacc taaaaaatca gggaatactg
     7741 gaggtaaaaa gaccagaact ggtaatttag atgatttcct taagagcgat tttacaaaag
     7801 gtgattttcc gaaggggatt aaaaggaaac ctcaaggaaa tgtttattat acgttatgta
     7861 gtaaatgtaa tagttttttt ggtagtgagt atgttgagga atatattaga tttgcagaag
     7921 acaataaaaa tttcctttat aacaatacaa gtttaaagaa tggtaagtct gatttaacac
     7981 attcaataaa gaaaatgaat tcacttagag ttgcaaaagc aattgtagca atgtttttta
     8041 gtttaaatgg ggatgaagat tcaatggata agccattctt agattcggta cgagaatatc
     8101 ttagcaatcc taaaagtact ttatttcctc atgaagacta caaaattata atgaattatt
     8161 atcttccaat aggtgctcca cagaattcta aaatcaaaat tcctaatgac attgtaaatg
     8221 atggtgacac tcacaatgta ataaaaggtt ttatgcccct tgatctatca actgggagaa
     8281 aaattcgatt ttcagaaatt cagtatgaag gaattggttt tactcttata gatttaaaaa
     8341 attcaacttt tgaaataaaa gaaggattag atttaaaaaa tctcctttgt gcagatgata
     8401 aaatcaaaaa aatacaattg tacaaagtcc ctatattaac accaacagta cttacaatgt
     8461 tggagatgga aaataatatt aattatattg actcaggttc agatttgctg attaggtggc
     8521 aggtaataac taattttctt atggaattaa aaaaatacag taatactcaa acgaagttct
     8581 cttgaagtta taaagaatac aataaatata aatttccttt agactatatt aaaatcagca
     8641 atcgaaggaa aataaacgaa gaaaatggta cgcaaaatct aactattaga gcaaaaacag
     8701 gaagctgctt tggaagatag tcccgaattg tttccgatgt taaaagatcg tttaaatgaa
     8761 ttgactgaaa aacgaagaca gttacaaatt cgagagaatg aaatcttagg gatctttcaa
     8821 caacaagggg aaccgattca agtcaaagat gtccaacaga tattgactag tttggattgc
     8881 tttcttatcc agagtgagaa gaagcaagtt aaagctttgt atcacacctt tattgagaaa
     8941 atcacctttg atcctaatca gaagtagata ttgccgatcc cgatgagatt cacacctact
     9001 gtgatttgat aattgaatga gtaatatcaa atagccgtat ccaaaccaaa ggatatggct
     9061 atttttgtgt tagggatata cgtcacgtag cttgttatca agaataagtt tctttatttt
     9121 tttgcttttt ctaaaactga gtatgctaaa ataagagggt atatagcaac tttaaataag
     9181 agataggagt gtatcttttt gggtgcagaa ttaaatcaga agttatttag tgcggcagat
     9241 aatctccgta gtaaaatgga tgcgtcagaa tataaaaatt atttattggg gctgattttc
     9301 tataaatatt tatccgaccg tttgttggaa caagtggttc ttttagcaga tgaatcctta
     9361 gaggaatatg acacagtaag caaacaaacg atgttgtatc gtgaattact ttcagatgaa
     9421 gaatcaaaag aagatctgat tgcaacaatc gttgatattt tggggtatgc gatttcgcca
     9481 gagtatcttt tcaacgtctt agcggatcaa gcaaaacaag caacgttcca attgaatgat
     9541 ctaaacaaag cctttttagc aagtcatatt tatggaaaaa tactgtcggc aaaattcgaa
     9601 tagtgtcgag gaggtgctgt gatggaagaa ttaaaagaac gcattgtcaa ttatgtcata
     9661 gagaactctg agattaaaaa taaatggggg aacaatacag cacagaaaat atctgaaaaa
     9721 ttaaatgtta gtcgtaatat tgtctctcaa tatttgaatg aattcgtaaa agatggagaa
     9781 tttggaaaaa tcaatactcg tccagtaatc ttcttttctt taccggagaa agttccaaat
     9841 caacaaattt ttagctcttc agaggaagcg gaaaaatggt atgatagctc ttttagggtt
     9901 ggaatagaaa gggttatcgg aagtcaagga agtctgcgaa atgtaattgc tcaaattaaa
     9961 gcagcaatct actatccgcc aaatggctta cccattttac ttcatggcga gactggaact
    10021 ggaaaaagct atttagcaaa ttatgtgtat aaggatttgt tggccaataa ttatatagaa
    10081 gagaaggcat ctttcatttc agttaattgt agtgaatatg ccaataatcc agaacttttc
    10141 ttgtccaatt tttttgggca tgtaaaaggt gcatatacag gagccgaaga tggaagaaag
    10201 ggtttagcag aattagctaa taatggagtc ttatttttag atgaaattca tgcattgagc
    10261 aatgaatgtc aggagaaact ttttcagttt atggatacga ggaaatttca tcggatgggt
    10321 gataatgaaa actggtacac atcaagttgc tggattatat ttgcaacatc agaaccacct
    10381 gaggataaac tattatcaac tttgttgaga agaatacctg taaagctaac acttccaaca
    10441 ttagacagtc gttctcttta tgaaagaaaa cagttaatca agatattttt taaatcagaa
    10501 gaagaaagac tagcaaagaa aatactgata tctcaacaat tattagattt tttattaaac
    10561 tatcgcttta gtgaaaatgt tggcaatttg aaaaatatca ttcaattgac ctgtgccaat
    10621 gccttttatt tcttagatga tttggaagaa ctttatattc agatgtatca tttaccacaa
    10681 tgggtgaatt tcaacggcat gaaggttatg aaaagacaga atgaattaaa atatattcaa
    10741 atggatgatt tggaaaaagt agacgaagtt ttta
//
LOCUS       DAZUJO010000089        10756 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000089 DAZUJO010000000
VERSION     DAZUJO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.092"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG8OL_002421"
     CDS             complement(33..1076)
                     /locus_tag="ACG8OL_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4818360.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ACG8OL_002422"
     CDS             complement(1237..1920)
                     /locus_tag="ACG8OL_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFS4818361.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ACG8OL_002423"
     CDS             2115..3044
                     /locus_tag="ACG8OL_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS4818362.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8OL_002424"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ACG8OL_002424"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFS4818363.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8OL_002425"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ACG8OL_002425"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS4818364.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8OL_002426"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ACG8OL_002426"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS4818365.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ACG8OL_002427"
     CDS             5429..6232
                     /locus_tag="ACG8OL_002427"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS4818366.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ACG8OL_002428"
     CDS             6316..7863
                     /locus_tag="ACG8OL_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS4818367.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ACG8OL_002429"
     CDS             complement(8160..8357)
                     /locus_tag="ACG8OL_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818368.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ACG8OL_002430"
     CDS             complement(8433..8633)
                     /locus_tag="ACG8OL_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS4818369.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ACG8OL_002431"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ACG8OL_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..>10756)
                     /locus_tag="ACG8OL_002432"
     CDS             complement(9985..>10756)
                     /locus_tag="ACG8OL_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4818370.1"
                     /translation="NKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGIS
                     VSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAADITY
                     IPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTD
                     LGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEA
                     NRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT         3114 a         2109 c         2177 g         3356 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattc
//
LOCUS       DAZUJO010000090        10490 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000090 DAZUJO010000000
VERSION     DAZUJO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.093"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            204..353
                     /locus_tag="ACG8OL_002433"
     CDS             204..353
                     /locus_tag="ACG8OL_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS4818371.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            364..1878
                     /gene="dltA"
                     /locus_tag="ACG8OL_002434"
     CDS             364..1878
                     /gene="dltA"
                     /locus_tag="ACG8OL_002434"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS4818372.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1875..3080
                     /gene="dltB"
                     /locus_tag="ACG8OL_002435"
     CDS             1875..3080
                     /gene="dltB"
                     /locus_tag="ACG8OL_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS4818373.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3125..3358
                     /gene="dltC"
                     /locus_tag="ACG8OL_002436"
     CDS             3125..3358
                     /gene="dltC"
                     /locus_tag="ACG8OL_002436"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS4818374.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3361..4629
                     /gene="dltD"
                     /locus_tag="ACG8OL_002437"
     CDS             3361..4629
                     /gene="dltD"
                     /locus_tag="ACG8OL_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS4818375.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4770..5852
                     /locus_tag="ACG8OL_002438"
     CDS             4770..5852
                     /locus_tag="ACG8OL_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS4818376.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5852..6655
                     /locus_tag="ACG8OL_002439"
     CDS             5852..6655
                     /locus_tag="ACG8OL_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4818377.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6725..7465
                     /locus_tag="ACG8OL_002440"
     CDS             6725..7465
                     /locus_tag="ACG8OL_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4818378.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7476..8480
                     /locus_tag="ACG8OL_002441"
     CDS             7476..8480
                     /locus_tag="ACG8OL_002441"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS4818379.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8525..10369)
                     /locus_tag="ACG8OL_002442"
     CDS             complement(8525..10369)
                     /locus_tag="ACG8OL_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818380.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3389 a         1718 c         2106 g         3277 t
ORIGIN      
        1 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
       61 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
      121 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
      181 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
      241 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
      301 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
      361 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
      421 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
      481 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
      541 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
      601 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
      661 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
      721 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
      781 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
      841 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
      901 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
      961 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
     1021 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
     1081 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
     1141 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
     1201 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
     1261 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
     1321 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
     1381 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
     1441 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
     1501 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
     1561 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
     1621 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
     1681 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
     1741 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
     1801 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
     1861 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
     1921 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
     1981 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
     2041 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
     2101 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
     2161 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
     2221 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
     2281 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
     2341 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
     2401 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
     2461 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
     2521 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
     2581 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
     2641 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
     2701 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
     2761 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
     2821 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
     2881 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
     2941 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
     3001 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
     3061 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
     3121 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
     3181 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
     3241 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
     3301 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
     3361 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
     3421 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
     3481 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
     3541 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
     3601 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
     3661 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
     3721 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
     3781 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
     3841 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
     3901 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
     3961 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
     4021 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
     4081 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
     4141 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
     4201 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
     4261 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
     4321 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
     4381 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
     4441 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
     4501 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
     4561 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
     4621 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
     4681 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
     4741 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
     4801 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
     4861 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
     4921 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
     4981 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
     5041 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
     5101 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
     5161 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
     5221 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
     5281 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
     5341 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
     5401 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
     5461 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
     5521 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
     5581 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
     5641 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
     5701 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
     5761 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
     5821 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
     5881 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
     5941 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
     6001 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
     6061 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
     6121 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
     6181 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
     6241 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
     6301 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
     6361 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
     6421 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
     6481 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
     6541 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
     6601 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
     6661 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
     6721 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
     6781 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
     6841 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
     6901 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
     6961 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
     7021 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
     7081 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
     7141 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
     7201 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
     7261 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
     7321 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
     7381 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
     7441 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
     7501 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
     7561 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
     7621 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
     7681 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
     7741 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
     7801 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
     7861 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
     7921 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
     7981 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
     8041 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
     8101 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
     8161 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
     8221 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
     8281 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
     8341 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
     8401 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
     8461 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
     8521 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
     8581 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
     8641 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
     8701 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
     8761 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
     8821 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
     8881 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
     8941 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
     9001 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
     9061 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
     9121 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
     9181 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
     9241 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
     9301 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
     9361 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
     9421 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
     9481 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
     9541 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
     9601 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
     9661 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
     9721 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
     9781 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
     9841 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
     9901 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
     9961 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    10021 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    10081 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    10141 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    10201 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    10261 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    10321 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    10381 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    10441 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta
//
LOCUS       DAZUJO010000091        10318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000091 DAZUJO010000000
VERSION     DAZUJO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.094"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..904)
                     /locus_tag="ACG8OL_002443"
     CDS             complement(71..904)
                     /locus_tag="ACG8OL_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS4818381.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1208..3373)
                     /locus_tag="ACG8OL_002444"
     CDS             complement(1208..3373)
                     /locus_tag="ACG8OL_002444"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS4818382.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3633..5927
                     /locus_tag="ACG8OL_002445"
     CDS             3633..5927
                     /locus_tag="ACG8OL_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS4818383.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5920..6585
                     /gene="pgmB"
                     /locus_tag="ACG8OL_002446"
     CDS             5920..6585
                     /gene="pgmB"
                     /locus_tag="ACG8OL_002446"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS4818384.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6578..7585
                     /locus_tag="ACG8OL_002447"
     CDS             6578..7585
                     /locus_tag="ACG8OL_002447"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS4818385.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7607..8626
                     /locus_tag="ACG8OL_002448"
     CDS             7607..8626
                     /locus_tag="ACG8OL_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4818386.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACG8OL_002449"
     CDS             complement(8907..10274)
                     /gene="nhaC"
                     /locus_tag="ACG8OL_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS4818387.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT         3198 a         2242 c         1909 g         2969 t
ORIGIN      
        1 ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt tatgttatat
       61 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
      121 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      181 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      241 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      301 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      361 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      421 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      481 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgaa
      541 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      601 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      661 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      721 tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      781 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      841 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      901 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      961 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
     1021 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1081 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1141 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1201 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1261 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1321 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1381 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1441 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1501 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1561 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1621 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1681 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1741 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1801 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1861 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1921 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1981 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     2041 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2101 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2161 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2221 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2281 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2341 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2401 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2461 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2521 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2581 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2641 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2701 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2761 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2821 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2881 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2941 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     3001 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3061 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3121 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3181 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3241 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3301 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3361 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3421 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3481 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3541 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3601 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3661 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3721 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3781 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3841 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3901 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3961 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     4021 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4081 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4141 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4201 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4261 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4321 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4381 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4441 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4501 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4561 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4621 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4681 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4741 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4801 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4861 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4921 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4981 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     5041 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5101 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5161 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5221 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5281 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5341 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5401 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5461 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5521 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5581 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5641 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5701 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5761 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5821 tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct tattctttag
     5881 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5941 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     6001 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6061 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6121 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6181 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6241 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6301 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6361 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6421 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6481 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6541 cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag agatctccgt
     6601 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6661 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6721 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6781 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6841 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6901 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6961 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     7021 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7081 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7141 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7201 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7261 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7321 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7381 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7441 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7501 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7561 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7621 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7681 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7741 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7801 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7861 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7921 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7981 ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat accaaaacca
     8041 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8101 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8161 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8221 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8281 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8341 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt atggctatgt
     8401 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8461 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8521 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8581 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8641 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8701 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     8761 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
     8821 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
     8881 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
     8941 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg caagtaatgc
     9001 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
     9061 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
     9121 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
     9181 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
     9241 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
     9301 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
     9361 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
     9421 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
     9481 agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
     9541 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
     9601 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     9661 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     9721 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     9781 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     9841 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     9901 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     9961 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
    10021 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
    10081 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
    10141 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
    10201 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
    10261 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaa
//
LOCUS       DAZUJO010000092        10126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000092 DAZUJO010000000
VERSION     DAZUJO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.095"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..366)
                     /locus_tag="ACG8OL_002450"
     CDS             complement(<1..366)
                     /locus_tag="ACG8OL_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFS4818388.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIEADGLTLDI
                     WLRKKRDTQAAYTFLKRLHK"
     gene            complement(398..1072)
                     /locus_tag="ACG8OL_002451"
     CDS             complement(398..1072)
                     /locus_tag="ACG8OL_002451"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS4818389.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGGSVAKFS
                     KKSILV"
     gene            1415..1831
                     /locus_tag="ACG8OL_002452"
     CDS             1415..1831
                     /locus_tag="ACG8OL_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4818390.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1828..2298
                     /locus_tag="ACG8OL_002453"
     CDS             1828..2298
                     /locus_tag="ACG8OL_002453"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4818391.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            2312..3085
                     /locus_tag="ACG8OL_002454"
     CDS             2312..3085
                     /locus_tag="ACG8OL_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4818392.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            3099..3911
                     /locus_tag="ACG8OL_002455"
     CDS             3099..3911
                     /locus_tag="ACG8OL_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4818393.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3965..4930
                     /locus_tag="ACG8OL_002456"
     CDS             3965..4930
                     /locus_tag="ACG8OL_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4818394.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            5067..7790
                     /locus_tag="ACG8OL_002457"
     CDS             5067..7790
                     /locus_tag="ACG8OL_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4818395.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVGALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7803..8435
                     /locus_tag="ACG8OL_002458"
     CDS             7803..8435
                     /locus_tag="ACG8OL_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS4818396.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9334..>10126
                     /locus_tag="ACG8OL_002459"
     CDS             9334..>10126
                     /locus_tag="ACG8OL_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818397.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3398 a         1565 c         1959 g         3204 t
ORIGIN      
        1 tttatggagt cgttttaaga aagtataggc tgcttgcgta tcccgtttct ttcgtaacca
       61 gatatctaag gttaagccgt ccgcttcaat tgcacgataa agataatgcc aacgtccctt
      121 aattttgata taggtttcgt ccattttcca tgaatagaag gattgtctat ttttcttctt
      181 ccaaagataa tagaggactt tgctgtactc ttgtacccaa cgataaatcg tagtatgaca
      241 aacatttatt ccacgatcat ataacaattc ctgaacttca cgatagctta gattgtaacg
      301 caggtagtaa ccaacagcga caataatgac gtcttttttg aattgtttgc ctttaaaatg
      361 attcattact ctgtcctctc tgtctttttt ctcaatttta cactaaaata gattttttgg
      421 aaaactttgc aacagaaccg ccaatcatat ggaagccacc gattaggtat tttatgggtt
      481 tagtcggaaa tgtttcaaga gcaatttcca tcatatcgat aatgccatga tgactacagc
      541 cagtaaagac aatcagaccg tcttcttgct caattacgac caataattca tctgaaaaat
      601 catcataaac aaatttatca tccatctttt tgtataaatt cttggtggtc gagtttgctt
      661 tgcctagggg gatgatatgg aagctcggag caagttctgt tttcgttttt attgtatgta
      721 aattctcatg cattttaagg atttcatttt taggaatacc aacatacaga aacgtggtta
      781 aaatctttaa ataatggggt aaagttacac cttcagctgt ataaataggg gcgtttcctg
      841 attttaaata tcttaacccg ccggtgtggt cagaatgacc gtgtgaaata atcactgcct
      901 ccagatcatt caaagtttcc cccattttag tcgcattata gactaatttt ttattagcgc
      961 cagcatcgaa gagatatttc ttccctgagt agttaattaa gagagataac ccatgtccac
     1021 ttttaaagtt caaatttatt cttgagtttt caaataaaac cttgatttcc actataacat
     1081 ctcctttcac atgaacgtgt attcatatat tgtagcacat taagttctat tttttgttgt
     1141 aattaccgaa aaaaatatta atggacgctt tttacgcttt atttcgtatg tggaagaaaa
     1201 tttcttagga tgtggcgggg gattggggca aagctaaatt ccaattacag tgatcattta
     1261 attagacacc tttaaatacc cacactttca tcaacactat aatttaggga aagaaaagtg
     1321 ttggcatgat atagaaaaac ctttaattaa cagcgtttcc taaaattggc acgcatcttg
     1381 cttatagaaa agcgtaagga taggaggaat gaaaatggca actataattc ttgccagcca
     1441 tggggaattt gctcaaggat taaagcaaac agcgacaatg attattggag atagtgtacc
     1501 aattcatgcc ttgtcagcat ttagagatga agatgaatct attttggtac aaataaaaaa
     1561 acttttagcc acaatagata tagaagaaac ctacatttta actgatattc taggtggtag
     1621 tgtgaataat gatatgctca ctattattaa agagtatgag ggattgcaat taattactgg
     1681 aatgaactta cctttagtta tttctattgc aacctataat ggaaaaattg gggaaagtga
     1741 gctagaaaag cttattaaag agagtcaaca atcagtgatt gattgtagaa aattattagt
     1801 agaacaactt actggaggag atgacctatg attaagttag tacgtattga ccatcgtttg
     1861 ttgcacggac aggtagtatt ttcttggagt aaatcattaa atatcaatcg tattattatt
     1921 gccaataaag aggcagcgaa tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg
     1981 actggcatta aattaacaat tttttcagtt gaacagttac tagaaaaaat gcctaaaatc
     2041 gaaacactta acgaaaatat catgcttatt tttggtaata caaaagaaat tcgtcaattt
     2101 tgtgaagcct atcctaaagt acaagaaatc aactatggag ggataatcaa aaaagaaggt
     2161 tccaagcaat ttagtaatgc cattttcttg aatgaatctg agcaagaaga tgccaaaaaa
     2221 ttaaaagctc ttggcattaa acaatttatt caacaggtac caacttctaa aaaagaagat
     2281 ttgaatacaa tgttataaag gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt
     2341 aatcggtgtt ttttgtattt tagactcaag gcttttagga agaatgaatt ttgaacgacc
     2401 gttgattgtc agtacgcttg ttggtttggt acttggagac atggaaaaag gattaattgt
     2461 tggagcctct ttggaattga tgtccttagg attagtaaat atcggagctg ctgcaccacc
     2521 tgatatgaat atggcatcaa ttattgctac agcctttgcc attttatcaa atgcgaatgc
     2581 agaagcagca ttaacgattg ccattccaat ttcagtgtta ggtcaaatac taggtatctt
     2641 aatgcggacg attttgtcaa atttgactca cgttgcggat cgctttatcg aacaagggaa
     2701 atttagccaa gcaaggagaa ttcatatact ttatggctcg ggattatatg ctctcatgta
     2761 ttttattcct atttttctag caatttattt tggaacgaat cttgtttctc agattgttga
     2821 tgcgattcct gagtggctaa caaatggctt aacggttgca agtaaaattt taccagcata
     2881 tgggtttgcg ttgttattac aaacgatgtt aacgaaaaaa atggtgccat ttttattact
     2941 tggattttta attactgctt attctggatt gggaatcatt ggtattgcag ccttcgcatg
     3001 tattgttgcg tttgtcatgg ttcaagttca aggtaaaaac tcaaatggta accaaaatga
     3061 gccagatcca ttagatgact tataaggagg tagattaagt gaaagagaaa gatcaaaatg
     3121 catggaaata tcggcagttc ttatggcgtt catgggcaat tcaagcctca tggaactatg
     3181 aacgacaaat gaacatgggt tttatgtacg gaattgcacc aattctagat aacatttaca
     3241 agaatccaga agatgaagca atgaagaaag aagcttacga gcggcatatg atgtactaca
     3301 actgtacccc tcaaacaagt gcatttgtgt tagggttaac tgcttctatg gaggagcaat
     3361 actacgaaga ccaagaaaac tttaatccgg aatcaatcag tgcagtgaag acttctctga
     3421 tgggaccgct atctggtgtt ggtgattctt tctttcaagg aacgattcgg gtattagcct
     3481 ttggactagg aattaattta gcccaacaag gtagtatttt aggtccaatt ctagcaatgt
     3541 taatttcctt tgtaccttcc tttttagtta cttactatgg tgggaaaatt ggctataaca
     3601 tggggaataa atacttgtct aaactgtatc aagaaggttt gatggataaa gtaatgtatg
     3661 tttgctccat tgtggggttg atggtcattg gttcgatgat tgccagcatg atcgggatta
     3721 caacaccaat tcaaatgggt aaagcttttg tcttacaaga tgtattagat agtattatgc
     3781 cacaattttt accattagga ttaaccttct tcatgtactg gttattgcag aaaaaggtca
     3841 aaacaggatg gatgttggca atctgtttag tcggaggaat tatttttagt ttgttaggta
     3901 tcttacaata agggaataac caataaattt ggttgaataa tataaataag gaggaattca
     3961 attgatgaat tcaaaagaaa ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat
     4021 cgtatttgtt ggctgtggcg cttcaaaagc ggacctttat cctgcaaagt attttctaga
     4081 ccaaaatgcg aagcagttac ggattagtca ttacacagcc agcgaattta actatgcaac
     4141 accagcatct gtagatgagt caactgtcgt tatttcagct tctttaggag gtacgactcc
     4201 agaaacagtt gaagctaacg cagtagctaa agaaaaaggt gctacagtta ttagtctaac
     4261 acgaagcatt gactcaccgt taacaaaaga tgcagattat gtaatttatc atcgatttgc
     4321 agagaactat gctgctaaat tagaaaaaac aggctactgt ttagaattgg cagtagaact
     4381 tttacatcaa gtagaaggtt atgaccatta tgaagcaatg caagaaggat ttgctactat
     4441 ctatgatctt gcccaaaaaa gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga
     4501 acgctttaaa gatgaaaaag ttatttatgt aatgagtagt ggtgctacac atgaagtagc
     4561 atactcaaca tcaatttgct tgatgatgga aatgcaatgg attaattctg gtaccttcca
     4621 ttcaggagaa tttttccacg gaccattcga aattgtggat aaagacgtac cgtttatatt
     4681 attaatgaat gatggaaaaa ctagaaatat agatgctcga gcattaactt tccttgaacg
     4741 ctttgatacg aaaacagaag ttgtagatgc cttggattat ggcttgtctt cacatattgc
     4801 taaagaagtc ttagactact ttaacccatt cgtgattact gcagtattta gagtttatgc
     4861 agaagaattg tcagaagcaa gagaacatcc attaactaag agacgttata tgtggaaact
     4921 aacttattaa aaaatagatc aaactaaata aggtggcttg agaaattaag ccaccttgtt
     4981 ttcttcaatg tgattttata catatggtaa aataactagt acatatggta aaataactag
     5041 tacatattgt gaaagcaggt ggcaagatgc agcgattaaa tgactatctt tatcaactaa
     5101 cattgctttt gaaatctacg ccttcagata attattcagc tcgcagtatt tcgcaagctt
     5161 tacagttgaa tagaagtaca atcagtagct acttaaatga aggtgttcgt gaagggcttt
     5221 tcataaaagt aaaaagttac ccggttttat ttttacatag aacagctctt gaagaactac
     5281 atatcacact gaataactcc gaaatagaat caattgaaag cttgttaaca atttctcaaa
     5341 aacccgcatt agatcaagta attggctcta aaggaagttt gaaagaagca attgatcaaa
     5401 ttaaaacggc agtcttatat cctggaaaag ggttgccttt attattgatc ggcgctagtg
     5461 gatcagggaa aacctttcta gcgaataaaa tatatgaata tgcagtagaa gaaaaggtca
     5521 ttcacacatc ggcacctttc atagattata attgtgcaca atatgtgagc aatccagagt
     5581 tactatcctc tgctttattt ggctacacga aaggagcatt taccggagca agccaggagc
     5641 atactggttt gctagaaaaa gccgatggag gagtattgtt tttagatgaa gtgcatcgct
     5701 tatctgaaga agggcaagag aaattattta catttatgga tacgggtgaa ttctctccta
     5761 tgggggataa tagtattcgg aaaaaagccg atgttcgatt agtctttgca acaacagaaa
     5821 atatatatac gactttctta ccaacttttc ttaggagatt gccagttatt gttaatctac
     5881 ctagatttca acaacgtccg tcgtttgaac gtctgtcatt gattgatgaa ttttttgttt
     5941 ctgagagtaa aattttagct aaggaactat ctgtttctga tgcattgatt catttcttaa
     6001 tgaatagtga ttttgaaggt aacgtgggga atattaaaaa tattatcaag tatgcttgcg
     6061 gaaacgcata tatccaccag aagagtcagc aaacaattca agttaaatta ttggatatgc
     6121 ctttagagta cagttataaa ttcaaggagc aactaaataa acccagaaag aaaagagcag
     6181 atcgaatcta ctatccagaa gaaacacgac aagtgcaact agaaagaaac aatcagcttt
     6241 ttgttcaatt ttttgaccaa ttacttactg gattctctga agtgattgaa aataacaagg
     6301 atattaaact atttttagaa gaaatggtta ccaaggtaac acaagtaatg gatacgctta
     6361 tttttcaaga ggattacgaa aaagagcagt ctttatacgc cattctaaac tatcatattc
     6421 gtcaaagtat tgattttatg cgagaaagct acgggtttga acaagatggt aaccgggtag
     6481 gcgctttggc caactatcta tatgtcaagg ataatcaagt gatttttgaa aatagtcttc
     6541 aagaaaagaa aaccaaagat cagcttaacg aattttttgc taatgttctt gaaacccctt
     6601 attggtatgc aaaaaaaatc ttaagccttt tatctgagtg tttagatcaa cctgtgtatg
     6661 aagaggacat tttctttatt acgctatatt tttatagttt gaatgtcacg aatgttaaaa
     6721 caggtattaa gagtattgtg ctagctcatg gttattctac agctagtagt atggcgaatg
     6781 ttgccaaccg tatgctgaat aagaacgttt ttcaagctta tgatatgcct ttagatatta
     6841 cattagagaa aattgaagca aaattgattc aattccttgg agattatcga acagattcag
     6901 gcttgatttt attggtagat atgggttcac taaaccaact aggcggttta ttagtgaatc
     6961 atctaaatgg accactgtta atcattgatc atatcaatac accgttatta ttggaagtcg
     7021 gccaacaagt attaaacaat aaaagtatca ccgaaatagg agcagaaatt gaaacaagtc
     7081 ttactttcaa aaagcagtta ctacttccca agaaaaagaa aaagaaagcc atcattactt
     7141 gttgttatac agggattggt agtgcgaccc agattcagga gattttaacg aaatgcttga
     7201 aagatgcagt tagagaatta gtaattattc cgtatgatta caaaaagctg ttagaaaaca
     7261 agaattttga gactcctttt caattatacg atgtcatcat gattgtaggt acagaagatc
     7321 ccaaaattaa tcagattcca tatattggat tagatcgcct gattaatggg gaagcaattg
     7381 gcgaatttgt tcattacttg gaaaaagagc tgactattga agtgaacgat ttacaaaaag
     7441 aattaatttt caatttttca attagtaaaa ttgtcgaaaa tttaaccatt ttggatccag
     7501 ataaggtgct atcttttgta caaaaagcaa ttaaagaatt atcgaagtta ttaaattttg
     7561 atttttctaa taatcagctg tttttgttgt accttcactg tagttgtatg attgaacgaa
     7621 ttgtacgtaa agaaagtgtg gatgagcaag aggacattga agaatatatg caagcaaatg
     7681 gagaaataat ggataaaatt catagcgctt tttttgagat tgaaagggaa tacacaattg
     7741 aaataccaga tttagagttg cgattattga gcgatattgt tcaaggatag gagtgaattc
     7801 gaatgacaat gggagtaatt tttgatatgg atggggtact catagacagc gagacatttt
     7861 attttgagag aagaatgaac ttctttcaag aactggagat agatccagga tcaaaccaga
     7921 ttgatgatta tgtggggaaa acagaagctg caatatggga aacattagtt cctgaggaca
     7981 aatcattaag agaggaactt tatccgaaat atgtagatta tcgaaaaacg catccaattg
     8041 atttcgctaa agatttacga aaagaagtga aaccgttact tgacttcctc aagaagcggg
     8101 atataccatt ggcactggca tcttcttctc cagttgttga gatagagagt atgctcattc
     8161 aatgtgaact tactgaatat ttttcctttg ttataagtgg tgagtcatta aaacaaagca
     8221 aaccacatcc cgaaatatat ttaaaagcga aacagcaatt agactgtgac ttctttattg
     8281 cagtagagga ttcaacgatt ggtattaaat cagcaaaggc agcaaatatc tatacggtgg
     8341 cactaaaaca accaatgcaa atcgatcaga gtgaagcaga catacaaatt gaaagtctac
     8401 aagaactgga acaaattgtt ttagaaaaag gttagtgaat taatggaaat aaggctatat
     8461 atctgtgcta gctattttga ctaaccaaaa aaacttgcta taattcaggt gtagtaaaag
     8521 gtgctggtaa caccttttac gaaaatgctt cggcagtgat aatgggattg gctttttaag
     8581 ttttttattt tgttcgcaat ccctaaggtc atgtgaatga tcctagggat tgctagaata
     8641 ttttgcaaca ttctatctat ttcagaacgt aaccttcccc agttcattgg gattcattag
     8701 catacaacca acatttcaaa aagaaattgc actacaccat tattagctat tttgactaac
     8761 cccaaaaaac ttgctataat tcaggtgtag gtaaaaggtg ctggtatcac cttttacgaa
     8821 actgcttcgg cagtggtaat gagtgtggct taaaggacta gtcctttaag ctttttactt
     8881 tgttcgcaat ctctaagatc aagttaatga tcgataagat tgctagaata ttttgcaaca
     8941 ttctatttca gaacgtaacc tttcccattc cactgtgatt cattggcaac cctcctcaca
     9001 accaacgttt caaaagaaat tgtaaagttg ttggggtacc acgaccgaac aatactacac
     9061 ctgaatgata gtaagttgtc tcattaagta tagcatatcc tctttttggg taaatagaaa
     9121 tcggttacat tttttatcca agaccaagtt ttttcatttg gtcttatttg ctatacccaa
     9181 acaattccgc agtatagtcg acggtattga ttttatcagg tgagtaatag aaacttgttt
     9241 tccttcccca aaacattacc tataaaggag aaaaaatggt atcgtaatta tataggtata
     9301 ggatgaaatt tttttctaag aggagggttg ttgatgaaaa atttacagtt aaaatttatt
     9361 gttgatccat cgattgtacg ctggtttcgg attatcaatc aatttgagcg caatcaactt
     9421 gccacaaaag gagaattagc aaagagtaat catgtatctg aacgaaccat tcaaacagat
     9481 gtcaataaaa tgaaggacta cttttctgaa agtgttcaat ttgtttcaac gaaaagtggg
     9541 tattcttttc agaaggaaag accggttctt tttctcaagc agaaggagca actactggag
     9601 aacgaaattt tatttgagtt gttagggaat gtcttttatg gcgaattgga agatatgacg
     9661 gagctaattg atcgttttca tttatctgaa gcgacttttc gtcggtattt gaagcagatt
     9721 caaccagttt tggaagaata cggcttagaa ttgagtcttt atcctcttga tttaaaagga
     9781 gaagaggcaa atattcgcaa gttttttaaa gacttctatt atgaagggga gatgacgccc
     9841 catactcttg ttccaccaag ggatcttcat gaacttgtgc aagctacatt ctatgataaa
     9901 tttgagtatt tctcgattgg aacagggact tctcctgctg aattttatta tagtttgtat
     9961 attgcgattg agcgtgtgag acaaggaaaa acgatagcca ttcctcaagg tctgattgag
    10021 cgagtaattg ctagtgagaa ttttcgctta atgtattcct tgaaagaagc gattcaaaca
    10081 aattatcaag ttgctttatc agaagaggag ttttgttggt tgtatt
//
LOCUS       DAZUJO010000093        10038 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000093 DAZUJO010000000
VERSION     DAZUJO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.096"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(504..728)
                     /locus_tag="ACG8OL_002460"
     CDS             complement(504..728)
                     /locus_tag="ACG8OL_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818398.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            919..3696
                     /locus_tag="ACG8OL_002461"
     CDS             919..3696
                     /locus_tag="ACG8OL_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4818399.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3777..4196
                     /locus_tag="ACG8OL_002462"
     CDS             3777..4196
                     /locus_tag="ACG8OL_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4818400.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4208..4678
                     /locus_tag="ACG8OL_002463"
     CDS             4208..4678
                     /locus_tag="ACG8OL_002463"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS4818401.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4695..5474
                     /locus_tag="ACG8OL_002464"
     CDS             4695..5474
                     /locus_tag="ACG8OL_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4818402.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5461..6282
                     /locus_tag="ACG8OL_002465"
     CDS             5461..6282
                     /locus_tag="ACG8OL_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4818403.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6299..7348
                     /locus_tag="ACG8OL_002466"
     CDS             6299..7348
                     /locus_tag="ACG8OL_002466"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4818404.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7361..8365
                     /locus_tag="ACG8OL_002467"
     CDS             7361..8365
                     /locus_tag="ACG8OL_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4818405.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8998..10017
                     /locus_tag="ACG8OL_002468"
     CDS             8998..10017
                     /locus_tag="ACG8OL_002468"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS4818406.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3364 a         1656 c         2093 g         2925 t
ORIGIN      
        1 ctctatttac ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa
       61 aaaagtgggt tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa
      121 tatttattcg tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat
      181 ggtttggcgc agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca
      241 aaagccactt gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg
      301 tttctacctt gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa
      361 aaaggatggt gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc
      421 attatgccaa taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac
      481 aggaaagttg aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc
      541 aagataaaga tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa
      601 cgagtttgtt ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa
      661 gatccgatga tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc
      721 gatttcattg aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa
      781 actggaagga actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc
      841 tataataaga tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt
      901 ttctatgatg gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag
      961 aaccagacag cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta
     1021 gctgatcatt ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat
     1081 gagcaactac tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt
     1141 gaaaaacaat tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag
     1201 gaacagcctt ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc
     1261 agtttgaaag aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta
     1321 ccacttttga ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat
     1381 cagtattgtc tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt
     1441 gcacaatatg cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga
     1501 gcgtacactg gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc
     1561 ttgtttttag acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat
     1621 cttgatcaag gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca
     1681 cgtctctttt ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga
     1741 agaataccta tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta
     1801 gaactgattt actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc
     1861 agtggaccag ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg
     1921 aaaaatatcg tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa
     1981 atccatgtca cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat
     2041 acagcacagc aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag
     2101 caacagcccg aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag
     2161 aaaaataact gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta
     2221 tttgattttt tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg
     2281 acacaatcta tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat
     2341 ggaaacagtg tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta
     2401 ccggaagaac atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct
     2461 tatccatcta gttatcacta tgtccaacga attctggaat taagtaagat caaattagat
     2521 attgaagaca caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg
     2581 tcaaaagaaa aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc
     2641 agtatggcaa atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg
     2701 ccactagatg tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc
     2761 gatgtttcaa atggattgat catattagta gacatgggat ctctgaaaga aatccatcag
     2821 ttttttaaaa aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta
     2881 gcaatcacag taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag
     2941 actgttcgac agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa
     3001 gcactcatta ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta
     3061 gaaaaaagct tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta
     3121 aaagataaga aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc
     3181 accacagatc ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg
     3241 gaaaagatgt ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa
     3301 atattcaatc agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact
     3361 attctagata cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg
     3421 cagttgaatc gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt
     3481 ctcattgaac gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg
     3541 aagcagtgtc aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag
     3601 cattatagtg tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa
     3661 gatacagagt ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa
     3721 ttttggcatg caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg
     3781 atagagaaat tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag
     3841 agctgatcta tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt
     3901 ttgatttagc ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg
     3961 ttctgacgga tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg
     4021 atcatgtcta tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt
     4081 tggacgctga agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa
     4141 tgatccagtt ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg
     4201 aagaaaaatg attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt
     4261 ttcatggaca caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac
     4321 gaatgagata cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat
     4381 caaatcaatt gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt
     4441 attcatcgta gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat
     4501 caaaaccatc aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc
     4561 ggtaaatatt ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga
     4621 agtagaaatc cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga
     4681 aaggaagaac ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac
     4741 aatcagaata tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta
     4801 caggaatcgt gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag
     4861 catttatcgg atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga
     4921 ttttagggac ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag
     4981 gtttgccgat tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc
     5041 caattatgaa ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc
     5101 atatgcactt gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt
     5161 cttatatggt gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc
     5221 aaaaggggtt gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc
     5281 gtttgctgat caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt
     5341 acttagagat tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag
     5401 tcaatattat gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag
     5461 atgaagaatt ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt
     5521 tggcgttctt ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat
     5581 gtttatgcaa tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca
     5641 gctttgaaac ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta
     5701 ggaatcacgg ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg
     5761 atcgatagta tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt
     5821 tggggaactt tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat
     5881 attttaggcc ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat
     5941 ctagcaacag gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac
     6001 ggtatgatgg aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca
     6061 atgactgcca cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct
     6121 gtgactgttc aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt
     6181 ggcatagtgt tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta
     6241 gctttctttg gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat
     6301 gtttacgatg caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa
     6361 gaatgatttt tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac
     6421 gggttcatct tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct
     6481 tactgtcgat attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat
     6541 cgatactgtg attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa
     6601 aatgatacaa aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact
     6661 tgctttagaa gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt
     6721 tacaaaaggc tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat
     6781 aagtaaaaat aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat
     6841 aatcaaccat ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag
     6901 tttatttcgt ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc
     6961 taaagaagca gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt
     7021 agaagcttat atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat
     7081 tgaaggggaa agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta
     7141 cgttggagag actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt
     7201 agcaatagaa tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt
     7261 cgcttataaa accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt
     7321 tgatacagtc ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga
     7381 agaacaaatc aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat
     7441 cgataaactt tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta
     7501 tgcttcctcg atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt
     7561 acagcatgct gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt
     7621 tattttgtcc tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa
     7681 gaaagtagga gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct
     7741 atgcgatttt gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc
     7801 agcagatcga ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca
     7861 gctcgaacgt tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg
     7921 cttatcattt gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa
     7981 tcaatgggga gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc
     8041 atcgaaatct atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac
     8101 aaccccagtc acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc
     8161 taaacttttg ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt
     8221 agaaggtatc agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt
     8281 gacacaacgc atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg
     8341 ctactatcgt cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga
     8401 gagcagcgat aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa
     8461 cacatttttt gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc
     8521 caaccatttt ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt
     8581 ccaagtacaa cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa
     8641 caaaagttcc tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat
     8701 ttagaaatgt tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc
     8761 ctaacctcct aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca
     8821 agcaaagcat aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt
     8881 tcaagtaaaa acgataaaag aagtaaacaa acacttaaaa acaatggaaa ttagtcccaa
     8941 ctttaagaaa tgtatactat aatggaaatt atcaattatt tatttggagg aatatccatg
     9001 aaacaaaaaa tcgcagttct tggtcctgga tcttggggca ctgctttagc acaaacattg
     9061 gcagaaaacg ggcatgatgt ccgtatttgg ggaaatgtac ctgaacagat tgatgaaatc
     9121 aataagtatc atacaaatca gcatttttta cctgatttga cgatcccaga atcgattatc
     9181 ggatataaag aattggcaga agcagtggat aacgcagatg ctattttatt tgttgttcca
     9241 acaaaagcga ttcgttcagt cgcacaggaa cttattggta aaatgaatac taagccaatc
     9301 attatacatg caagtaaagg actagaacaa gatacgcata aacgaatctc agaagttttg
     9361 gctgaagaaa ttccagaaga aaaaagacag gcaattgttg tattatctgg tcctagccat
     9421 gctgaggaag tggcagtaca tgatatcaca acaattacag ctgcaagtat tgatcaaaaa
     9481 gcagcagcct atgtacaaaa actgttcatg aatgattatt tcaggattta tacaaataat
     9541 gatgtgattg gcgtagaaac tggtgcagca ctgaaaaata tcattgcaat tggtgccggc
     9601 gctatccatg gattaggatt cggagatgat gcaaaagcag caatcatgac tcgaggattg
     9661 gcagaaatca gccgtcttgg tgtcgcgatg ggtgccaatc ctctgacatt tatcggtttg
     9721 agtggtgtgg gggacttgat cgttacttgt acaagtgttc attctcgcaa ttggcgtgcc
     9781 ggtaacttgt taggacaggg acataagctg gaagaagttc tagaaaacat gggaatggtc
     9841 gttgagggtg tggcaacgac aaaagcagca gtagagctag ctcaacagct aggtgttgag
     9901 atgccaatta ctcaaacaat ctataatgtt ttgtataatg gagaagatat caaaaaagca
     9961 gcaaaagaaa tcatgttacg tgacggaaaa atggagaatg aattttctct tcgttaaata
    10021 gatgatcaat agttaatc
//
LOCUS       DAZUJO010000094         9893 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000094 DAZUJO010000000
VERSION     DAZUJO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.098"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..566)
                     /locus_tag="ACG8OL_002469"
     CDS             complement(54..566)
                     /locus_tag="ACG8OL_002469"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS4818407.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(580..2877)
                     /gene="recJ"
                     /locus_tag="ACG8OL_002470"
     CDS             complement(580..2877)
                     /gene="recJ"
                     /locus_tag="ACG8OL_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS4818408.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3019..3462)
                     /locus_tag="ACG8OL_002471"
     CDS             complement(3019..3462)
                     /locus_tag="ACG8OL_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS4818409.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3463..4251)
                     /locus_tag="ACG8OL_002472"
     CDS             complement(3463..4251)
                     /locus_tag="ACG8OL_002472"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS4818410.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4248..5189)
                     /gene="rnz"
                     /locus_tag="ACG8OL_002473"
     CDS             complement(4248..5189)
                     /gene="rnz"
                     /locus_tag="ACG8OL_002473"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS4818411.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5366..5953)
                     /locus_tag="ACG8OL_002474"
     CDS             complement(5366..5953)
                     /locus_tag="ACG8OL_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS4818412.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6174..7487)
                     /gene="obgE"
                     /locus_tag="ACG8OL_002475"
     CDS             complement(6174..7487)
                     /gene="obgE"
                     /locus_tag="ACG8OL_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS4818413.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7775..8785
                     /gene="gap"
                     /locus_tag="ACG8OL_002476"
     CDS             7775..8785
                     /gene="gap"
                     /locus_tag="ACG8OL_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS4818414.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8870..9805)
                     /locus_tag="ACG8OL_002477"
     CDS             complement(8870..9805)
                     /locus_tag="ACG8OL_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS4818415.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2721 a         2267 c         1708 g         3197 t
ORIGIN      
        1 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
       61 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
      121 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
      181 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
      241 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
      301 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
      361 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
      421 attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata agcgtcacca
      481 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
      541 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
      601 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgtcg
      661 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
      721 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
      781 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
      841 ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
      901 caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg aataagaccg
      961 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta gagagtttgg
     1021 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     1081 ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat aaaggctacc
     1141 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     1201 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     1261 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     1321 accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt tcagatgctg
     1381 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     1441 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     1501 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     1561 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     1621 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     1681 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     1741 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     1801 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     1861 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     1921 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     1981 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     2041 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     2101 cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg aaacaaggat
     2161 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     2221 aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     2281 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     2341 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     2401 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     2461 gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc catgctcaaa
     2521 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     2581 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     2641 gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca gatacggatc
     2701 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     2761 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     2821 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     2881 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     2941 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     3001 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     3061 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     3121 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     3181 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     3241 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     3301 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     3361 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     3421 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     3481 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     3541 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     3601 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     3661 atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg taacagctac
     3721 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     3781 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg cgatattaat
     3841 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     3901 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     3961 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     4021 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     4081 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     4141 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     4201 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     4261 tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt gttttcaaac
     4321 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     4381 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     4441 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     4501 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     4561 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     4621 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     4681 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     4741 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     4801 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     4861 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     4921 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     4981 aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct cggacgaata
     5041 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     5101 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     5161 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     5221 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     5281 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     5341 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     5401 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     5461 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga gtaatttctc
     5521 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     5581 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     5641 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     5701 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     5761 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
     5821 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
     5881 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
     5941 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
     6001 tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
     6061 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
     6121 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
     6181 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
     6241 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
     6301 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
     6361 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
     6421 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
     6481 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
     6541 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
     6601 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
     6661 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
     6721 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
     6781 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
     6841 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggt
     6901 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
     6961 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
     7021 tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc cggtgcagga
     7081 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
     7141 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
     7201 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
     7261 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
     7321 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
     7381 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
     7441 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
     7501 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
     7561 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
     7621 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
     7681 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
     7741 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
     7801 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
     7861 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
     7921 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
     7981 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
     8041 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
     8101 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
     8161 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
     8221 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
     8281 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
     8341 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
     8401 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
     8461 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
     8521 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
     8581 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
     8641 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
     8701 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
     8761 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
     8821 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
     8881 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
     8941 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
     9001 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
     9061 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
     9121 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
     9181 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
     9241 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
     9301 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
     9361 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
     9421 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
     9481 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
     9541 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
     9601 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
     9661 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
     9721 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
     9781 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
     9841 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DAZUJO010000095         9874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000095 DAZUJO010000000
VERSION     DAZUJO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.099"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..267)
                     /locus_tag="ACG8OL_002478"
     CDS             complement(22..267)
                     /locus_tag="ACG8OL_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS4818416.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(382..960)
                     /locus_tag="ACG8OL_002479"
     CDS             complement(382..960)
                     /locus_tag="ACG8OL_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS4818417.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1037..1567)
                     /locus_tag="ACG8OL_002480"
     CDS             complement(1037..1567)
                     /locus_tag="ACG8OL_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS4818418.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1571..2431)
                     /gene="rlmB"
                     /locus_tag="ACG8OL_002481"
     CDS             complement(1571..2431)
                     /gene="rlmB"
                     /locus_tag="ACG8OL_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS4818419.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2424..2819)
                     /locus_tag="ACG8OL_002482"
     CDS             complement(2424..2819)
                     /locus_tag="ACG8OL_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS4818420.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2816..4228)
                     /gene="cysS"
                     /locus_tag="ACG8OL_002483"
     CDS             complement(2816..4228)
                     /gene="cysS"
                     /locus_tag="ACG8OL_002483"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS4818421.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4225..4761)
                     /gene="epsC"
                     /locus_tag="ACG8OL_002484"
     CDS             complement(4225..4761)
                     /gene="epsC"
                     /locus_tag="ACG8OL_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS4818422.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4847..5086)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5083..6543)
                     /gene="gltX"
                     /locus_tag="ACG8OL_002485"
     CDS             complement(5083..6543)
                     /gene="gltX"
                     /locus_tag="ACG8OL_002485"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS4818423.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6713..7870)
                     /locus_tag="ACG8OL_002486"
     CDS             complement(6713..7870)
                     /locus_tag="ACG8OL_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS4818424.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7904..9274)
                     /gene="radA"
                     /locus_tag="ACG8OL_002487"
     CDS             complement(7904..9274)
                     /gene="radA"
                     /locus_tag="ACG8OL_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS4818425.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9337..9864)
                     /locus_tag="ACG8OL_002488"
     CDS             complement(9337..9864)
                     /locus_tag="ACG8OL_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS4818426.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2509 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaaagaaat gcaataaaaa taaataagaa
      361 gctcattaag atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt
      421 gagttttact ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc
      481 catgtctaca aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga
      541 aaactggatt tcgcaatttt cgattgcttc ttttaataaa agatgctgaa tcgctaaagg
      601 ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat
      661 tgagacttca tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata
      721 gatttgattg cttataatgc ttttataata acttccaaag ctaacacctt tgcttttttc
      781 gtaagttctc aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac
      841 ataatttctt aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata
      901 cagggcagca aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat
      961 aaaataattc ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac
     1021 tgattagaac gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt
     1081 tttttaactg ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt
     1141 tattttcttg ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag
     1201 aagttcgtaa tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca
     1261 ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag
     1321 ccgtttcgtc ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga
     1381 tgcccggaac tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat
     1441 aatttgataa acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa
     1501 gctggacaag ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt
     1561 ttttcatggt ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag
     1621 caacactggc atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca
     1681 tttctttttt caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg
     1741 atcctttggc attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa
     1801 atcctgtttc tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga
     1861 catgttcgac tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt
     1921 gttttggtat gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa
     1981 aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt
     2041 ttgttcgttc aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga
     2101 ctccttgatg cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga
     2161 ctggcacagc ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat
     2221 cttcttgaag aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt
     2281 gcccaaagac gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc
     2341 gtccagtttg ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg
     2401 attgatgttt gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag
     2461 cggatcaatt cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct
     2521 tcgaaccctg ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt
     2581 ttagcatttc ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc
     2641 agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga
     2701 tgcagcttat tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg
     2761 taaatcgcat ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg
     2821 cttctcttct ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca
     2881 acaaatcacg aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt
     2941 cttcgatcaa ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga
     3001 agatcgaaag ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt
     3061 tcggctggtc tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat
     3121 ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca
     3181 aagcgatttt atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca
     3241 aattgtcaaa cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt
     3301 cactgtagcg gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg
     3361 gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt
     3421 tttcatcgtc ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt
     3481 tccctgtttt cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt
     3541 gcccgccacc atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt
     3601 caatatgcca accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt
     3661 ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg
     3721 tacgctgact tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat
     3781 tttcaaattt acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt
     3841 caaccaacac ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca
     3901 gtgtagcagg ctcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat
     3961 cagcaacttc tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta
     4021 catccgtgaa attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa
     4081 ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg
     4141 gtccgcagac atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc
     4201 tggtcaatgt attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg
     4261 aacgactttt gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac
     4321 ggcacctgca ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt
     4381 cgcagagagc atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt
     4441 gccgcctaat gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc
     4501 aatgacgatt cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg
     4561 gatctcaaca ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag
     4621 ccggtgctga tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta
     4681 tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat
     4741 cgctcgggta aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat
     4801 catcttctta taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt
     4861 ccgcgtggtt ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat
     4921 acgttcttgg ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa
     4981 gaccactccc agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc
     5041 tcttgtaatg atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt
     5101 taaatgtgct aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc
     5161 atgcatttgt cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc
     5221 agtttctttt tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat
     5281 ttgttctagc tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac
     5341 ttcttttgct gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac
     5401 gatttctgca gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc
     5461 gattgtcttt tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca
     5521 catgtcagta aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag
     5581 ttttttcgca tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat
     5641 caattcttgg tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat
     5701 aaagttgaac attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa
     5761 gattgattcg tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat
     5821 atgtccgaag gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt
     5881 attggcaata tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac
     5941 cgcaacagca aaattataag ttggcatacc atcgcgtttt tgtatgacaa agtcgccacc
     6001 gatattatcg gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt
     6061 attgcgaggc acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg
     6121 ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg
     6181 ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt
     6241 gctagcaagc agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta
     6301 agggccgtat tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag
     6361 gttttccaac tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat
     6421 acgaataata aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct
     6481 tgcatttcca atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt
     6541 cattttcttc ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac
     6601 ggcttctcaa atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta
     6661 aatagtaaag ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg
     6721 attgtttagt ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag
     6781 gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga
     6841 tacgttcatt catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt
     6901 aggctactcc ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac
     6961 cagggatgac gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat
     7021 tttggaattc agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa
     7081 gcttagccaa tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt
     7141 acatttcgac agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac
     7201 gaccacgcac acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa
     7261 caaaattagg gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc
     7321 gaccgtcaat gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt
     7381 ttcgttctaa cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc
     7441 gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat
     7501 agcctaagat gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat
     7561 tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac
     7621 cgaagagaag ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag
     7681 ctgagatatg tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc
     7741 tgttggtaaa tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg
     7801 agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac
     7861 gtttttgcat cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct
     7921 tcaacgtttc agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt
     7981 tacctaagtt gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga
     8041 ctcgttgttc aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga
     8101 aacattccgt tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag
     8161 ctaaatcaat cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt
     8221 gattctgaag aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat
     8281 tgaaatcaag acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg
     8341 cttggatctc taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag
     8401 ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc
     8461 catgctcatg catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg
     8521 ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca
     8581 tatgttctaa cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa
     8641 aaatggctat cccatttgtt ttagcgattt tgagtagttc tgcagtggtt tctcttactt
     8701 ggctgacact tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa
     8761 tgataacata atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt
     8821 cagcgtatag ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct
     8881 gctctgcgct ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct
     8941 gagaaacttg taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca
     9001 tagaccctgg caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga
     9061 tccgaggttc ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg
     9121 tcaaacttgt ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat
     9181 tccattttcc gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt
     9241 ggcaaacaaa ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca
     9301 tgagacatta aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc
     9361 gccttgacgt tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc
     9421 ttggccgata cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg
     9481 gaacatgata tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac
     9541 tccgtttgtc aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa
     9601 aaattcatct tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg
     9661 tttttcattt tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt
     9721 ccagcctgct ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc
     9781 agcagcatga tgtgtagcgc gctgcggtag attgatattt tgatctttat atttagaaat
     9841 aacttcaaat cctctttttc tcaccggttt tcct
//
LOCUS       DAZUJO010000096         9864 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000096 DAZUJO010000000
VERSION     DAZUJO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.100"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..786
                     /locus_tag="ACG8OL_002489"
                     /pseudo
     CDS             278..786
                     /locus_tag="ACG8OL_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            879..2041
                     /locus_tag="ACG8OL_002490"
     CDS             join(879..1155,1155..2041)
                     /locus_tag="ACG8OL_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4818427.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2321..3301
                     /locus_tag="ACG8OL_002491"
     CDS             2321..3301
                     /locus_tag="ACG8OL_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4818428.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3423..4841
                     /locus_tag="ACG8OL_002492"
     CDS             3423..4841
                     /locus_tag="ACG8OL_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS4818429.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4912..6381
                     /locus_tag="ACG8OL_002493"
     CDS             4912..6381
                     /locus_tag="ACG8OL_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4818430.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6391..6858
                     /locus_tag="ACG8OL_002494"
     CDS             6391..6858
                     /locus_tag="ACG8OL_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4818431.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6894..7766
                     /gene="scrK"
                     /locus_tag="ACG8OL_002495"
     CDS             6894..7766
                     /gene="scrK"
                     /locus_tag="ACG8OL_002495"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4818432.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7847..>9864
                     /locus_tag="ACG8OL_002496"
     CDS             7847..>9864
                     /locus_tag="ACG8OL_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4818433.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEK"
BASE COUNT         3419 a         1430 c         1973 g         3042 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaggaaata agaaaactaa acttgtatcg
      601 ttaacaaaat atggtcgaaa ggtgtacgaa tcacataatt atttacatga agagctttat
      661 agctcaataa atgaagcgtt agaatcattc tcaccagacc aaatttcaac cattaaacag
      721 attctagaaa aagtatctta tacaatcaat gaatatcaag taagtctgac aagaaaggaa
      781 gattgacatg aaaaaaatga tttttgaatc ggctacacta gactagacag aaaaaataag
      841 gtgtgtaaaa taaatgaaaa cacactggag gaatttttat gtcaagacgt caacgtagaa
      901 cctattcaaa agaattcaaa caacaagtcg tcgatcttta tctcgcagga aagccccgag
      961 ctgaaatcat tcgagattat gagctgacta cctcggcctt tgataagtgg gtcaagcaag
     1021 cgcaaactac tggctcgttt caggaaaaag ataacttgat ccctgaacaa gtagaactaa
     1081 ttgcattacg aaagaaaaat aaacagcttg aaatggagaa tgacatttta aagcaagcgg
     1141 cgctgatatt gggacgaaaa gacaagtaat tgataccaat aagcacaaat actcaatatc
     1201 agcgatgtgt aaagtgttaa acatctcttg ccaaacctat tattatcaag ccaaaccagt
     1261 tgaagatgaa tcagaactgg aagaactcgt tcaagaagaa tttattcgca atcgaaaagc
     1321 ttatggtacc aggaagctga agcagtgctt ggccaatcta ggtcttcaag tgagtcaacg
     1381 cagaatcggc cgtatgatga aacagcgtgg cttgcgttca acatacacat tggctcattt
     1441 caaattccat tcttctactt gtaacgaagc caagaccgcc aatattctag acggaacctt
     1501 cctacaggaa aagtcattag aagcgattgt aaccgacctt acctatgttc gtgttgggaa
     1561 gaaatggcag tatatctgcc tgattcttga tttgtttaat cgagaaatca tcggctattc
     1621 atgcggggag aagaaagatg cgacgttagt caaggaagct tttgcaaaga ttccgtactc
     1681 gttgaataat gtacaacttt ttcacacgga tcgtggaaaa gaattcgata accagacgat
     1741 tgacgaaatt ttatgggcct ttaatatcac gcgttcctta agcaaaaaag gctgccccta
     1801 tgataacgcc gttgcagaat ccacctataa atctgtcaaa gtagaattcg ttcatcaata
     1861 ccgctttgag acattggcta atttgcgatt agagctattt gattacgtcc attggtggaa
     1921 taccatccgg ctgcacggca cgttaggcta tgagacaccg atcagttttc gacagcagag
     1981 attggcgcag cgaatccttg ataatgagct cggatgtgat accggtggcg aggcacctta
     2041 attatttgaa tgagcttctg ccttagagaa tcagattcga ggaggctcgt tgtcaagggc
     2101 agtcggaaca ccagcagcaa gcttacgcaa taccttataa tttttgtcaa aaaaagtgtt
     2161 gccattccac atatatcttt aaacagctat tgccagataa aaataaatga tattatatat
     2221 ataaatactt atccgaaaag gggaatgaga gagtcaaggt tcttggaatg tcgaatagtc
     2281 gagtaggtag attttctatc tacaaccttg ttgtttaaaa atgaaaaaaa ttggaattag
     2341 agaagttgcg aaagaggcaa atgtttctcc tacgactgtg tcgagggtat tgaatgatcg
     2401 tggatatttg agtgaggaaa caaaaagaaa agtttttgat gccatgaaaa aattagaata
     2461 ctatcctaat gaactggcga gagcactttt taaaaacgaa acatataccg taggtttgat
     2521 ttttcctaca attactaatc catttcacgc tgagttaatt caagatatcg agttcgagct
     2581 ttccagcagg ggatataaag tgttgctgtg caatagctta aacaatccaa caaaagaaaa
     2641 agagtacttg acgatgttga gaaagaatca ggttgatgga attatagtag gaacgcacaa
     2701 taaagatatt gcggattatg atattccagg gctacctatt gtagcgattg atagaagtct
     2761 tggtgaaaat acagttacag tatcttgtga taattacagg ggtggggaat tagctactca
     2821 actacttgta aatcatggct gtcagaatat actgtgtatt cggggagata gtaagataaa
     2881 gctacccgcg aatagtagga ctttagcata taaagatgtt ttgaataaat tcatgctgga
     2941 accgttagtc ctagaagtac catttgtaaa agaaatcaat gaaaaaaatg aaataattaa
     3001 gacatatctg gaaactcatc caaatattga cggggttttt gcaggagatg atttattggc
     3061 ttctttggcg attaacaact taggaagttc gggcaaaaga gttccaaaag aggttaaagt
     3121 gattggattt gatggagctc aacaaacatt gatttataat ccagaattat caactattca
     3181 acagcctata gatcaaatat ctaaagtagc agtaaaaaaa ttattgaata tgattaaggg
     3241 cgaaaaggag acggatgaac tttcacttcc agtaaagctc ataaaaaaaa atacagcata
     3301 agttaaatcg gcgaaaaatt ccttcgtcga tttaaaaata tttttatgaa acgggttgac
     3361 atatgaaacg ggttgacata tattagttgt gaagataaaa accttttcac tggaggaaga
     3421 aaatggagaa attattaatt aaaagcaata atcatcaact ttactaccaa cctaaggatg
     3481 tttgggtcgg agatgttatg ccttacggga aagaaggaaa attctttatt tatcatcaac
     3541 gtgatactag aaatccagtt ccttttggag aaccgtttgg ctgggcttta gcaactacga
     3601 aagattttgt tcattttgaa gactttgggg aatctctttt aagaggggat gacaatgaga
     3661 gagaccagtt tatctacgct ggttctgtat ttgaggcaaa tggaaaggtt cacgcatttt
     3721 atacagggta taatcgtgaa ttcttagcgc agggaaagac atctcaagtg ttgcttcatg
     3781 ctgagagtga tgattttgtt cattgggaaa aatcagaaac agcattggag ttagaaccac
     3841 aagaagggta tgatattcga aactggagag atccatttgt cgtttgggat gatgaaaaag
     3901 aagaatattt gctaatttta ggggcgcgaa agggtcagga taaacatcta caaaccggac
     3961 gtttagtgga atttacatca aaagatttaa ctaattggga attcaaaggt gatttttggg
     4021 ctccgggatt gtataccatg tttgaaatgc cagaactttt caagattggt gactggtggt
     4081 atcttgtcta ttcagaatac tcagatggaa ataaaatact ctatcgaatg agtcaaagtt
     4141 tggaaggtcc ttggattaag cctaaagatg aagcttttga tggacgggct tattatgcag
     4201 gtagaacagc ttatgatgga aaacgtcgag tcctatttgg atgggtacca acaaaagatc
     4261 aagaaaatga taaaaacaat tatttatggg gaggtacttt ggttcctcat gaagtctatc
     4321 aaaaagagga tttttcactt ggggtaaaac ccatagacga agtgtggaat gcattcataa
     4381 atttacatga tattgccgat gtagagatag aaacagcaga cacaagagaa gaagtaacgc
     4441 ttgtcaaaaa tagtggaact ttgtttaatt tagaaacaac aatttcattt gatgagaatc
     4501 ttagtgactt ttcactgaga ttatataaaa accttgagac agatgaatct tacgaatatc
     4561 gcttcaattt agatgagggg cgtcttacat ttgataaaaa cccttgttac ccttggtttc
     4621 aatatatgga taaaggactt attagaccta tatctttaaa gcctaaccaa tcttataaac
     4681 taaaagtgat tgttgatgac aatataatga cactttatat tgatggaatc gctcttaacg
     4741 ctcgtgtaca tggttgtttt ggtcaagcaa ttactattac agcaacaaat ggatcaatga
     4801 ctgcaataaa taccaagttt tcaaatgaaa taaagaaata ataaacgtca atcatgtggc
     4861 aggtctgaaa atcaaacatg acctgtcaca tttaaaaggg aggataaatg aatggactac
     4921 aaaaaaattg ccgaagatgt attggaaaac gttggaggaa aagagaatat ctcaagcgct
     4981 acacactgca ttactcgttt acgtttaatt cttaaagaca aaagcaaagc gaatatggaa
     5041 atgcttgagg aaatagaagg tgcaaaaggt gttcttttca atagtggaca gttacaagta
     5101 attttcggaa caggtataat tgaaaaggtt tacaaagagt tcgttgaatt gacaggagct
     5161 aaggaagtat ccgtttctga aatgaaagac gaaggagtca ataattcagg aaatagactt
     5221 caacgagctt ttaaagtttt ctcggatatc tttattccga tcattccagc ttttattggg
     5281 gcagcaatgg ttttaggaat tcgttcgttg attaccactc ctggaatgtt tggaatggat
     5341 ggctcattcg ctgatcacaa tgtattccta aatgatttat ccaaatttct gggtattatt
     5401 gcaaccacat ttgattattt accagtttta gttatgtatt ctgcaacaaa acgttttggc
     5461 ggaaacccta ttttaggatt acttgtagga tttgttatga ttcatccagg actagccaat
     5521 cggaatgatt tcgtcatggg aactataaag cctgattatt ggcatttatt tggattaagc
     5581 attccagcag ttgcgtttca agggggtgtt tttccagcaa ttttgacttc ttggttttta
     5641 gcaaaagttg agaaaattac aaataaatat tctcctcaag tacttgctta cattttagtc
     5701 ccaactgtga caattgttct tgccaacttt gcactatttt tagtgtttgg tccattgggg
     5761 aattttatcg gtaatgtttt aggtggaatc attgatttcc tatatatgaa gatgggtgcc
     5821 tttggtgctt ttgtttttgc aggcgcgtta caaccattag ttgtaacagg aacacaacat
     5881 gctattcaag gtattgaggc aaacttagtc gcaacaactg gattcaatta tatccaacct
     5941 atttggtcag tttcaattat tgcccaaggt ggcggttgta ttggtatgta tttactcttt
     6001 aagaaaaaat ctaaagatag agatattgcg atttcaagct ttattccaac actggtaggt
     6061 attactgaac cggctatctt tgctgttaat ttgaaatatt caatcgttcc ttttgtttgc
     6121 tcgtttattg gtgctggttt tggtggagca tttatgaaaa tttttgatgt aaaagccatt
     6181 ggacaaggat tgactgtatt gccaggtctg actattgcta atccagttct tccttatata
     6241 tttggtaatc tcatagcatt cattttacca atcattttta ttctcgctta tgataaatat
     6301 aaagtgttta tccctgaagc taaacaccgt aaatttgcag aaaaaactga ctcaaaaaaa
     6361 ttgccagttc aagtaaagtg agatgagagc atgttaaatt tttttagaaa aaatgaaata
     6421 agcgttgtag cacctgcaac cggtgttttt gaaaaactgg agaaattaag tgatccagtc
     6481 ttttcaaaag gaatgatggg acaaggattt gcaattgatg caaaaaacga aacgattgta
     6541 tctccaatcg atggttttgt ttcaagtatt tttcctacaa aacatgcctt aggaataaaa
     6601 acaaaatcag gaattgagat acttgttcat gtaggaatag atacagttga gctaaatggt
     6661 gagggatttg atattaaagt tcaagaagga caaaaagtta aggctgggga tattcttatg
     6721 accattaatt ttgtagtttt tgaaaagaat cacaagacta aagatgtcat tgttgttttt
     6781 ccagactacc aagacgaaat tttcattccg aaacttgaag aagtaaaaca aggacaagaa
     6841 ttgtttgttc ttggctaatg ataagtaatt aataatagaa tcgaggaaaa tgtatggttt
     6901 ttggggctat tgaagctggt ggaacaaaat ttgtttgtgc cattggagat aaagatttga
     6961 acatatttga aaaagtcagc tttcccacga ctacacccaa agaaacaatg aatttagttt
     7021 tagatttttt taatcatcat aaagatgatt tagaaggtat cggagtggga tcgttcggtc
     7081 ctattgatat tcattgtgaa tctaaaacgt atggcttcat taaatcaacg ccaaaacttg
     7141 cttggcagga ttttgatttt attggaagtt taaaaaagca acttaatatt ccaactgctt
     7201 ggacgactga tgtaaatgcc tcttgctatg gtgaatatgt ttctggaaat ggcagaggcc
     7261 tttcaagtgt tgtttactat accgtaggaa cgggcatagg aggtggcgct atccaaaacg
     7321 gaaacttcat tgaaggtttt agtcatccag aaatgggaca tatgcttgta aaacgacaca
     7381 ataaagacaa cttttctggg acatgtccat tccacggaga ctgtttagag ggcttagctg
     7441 ctggtccggc aatcgaaaga agatcaggga aaaagggaca agaagtgact gaaaatgacg
     7501 ttacttggga aatagaagct tattacattg ctcagtgtgc atataataca acattgatgt
     7561 tatcacctga tgtcattata tttggtggcg gagtaatgaa acaaaaacac ttaaaaaaga
     7621 aagtacaaga taagtttatt gagttgatca acggatatgt cgctattccc gatgttgaca
     7681 attatattat gacaccaaaa ttgttagata attctggtat tgtaggttgt ttagctttag
     7741 caagagacgt taaagaaaaa tcttaatttg gaaaaaagat cgtctcgact atttgtttgg
     7801 ttgatgcgat ctttttttca attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat
     7861 gaaagatgaa gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca
     7921 agatattgca atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt
     7981 attttcagat ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa
     8041 tcaacaaaag ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc
     8101 cactatagat aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat
     8161 aaaaggaaac agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct
     8221 tgatagaaaa gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc
     8281 aaataatcca gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg
     8341 ggctgaaaaa gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga
     8401 tgaggttcac aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca
     8461 aaagtatcgt aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt
     8521 agccacaaca gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc
     8581 tgaaatcact ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt
     8641 tgaatttctt aaagatgaag caattacgat tgattcaaaa atattttgtt cgattgaact
     8701 ttttactgat cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat
     8761 caagcttctt tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg
     8821 ggacaaacta gacagcggtt tttggataga tccagaaatt agtatagatt taaaaaaaat
     8881 gtttcttaaa tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat
     8941 tgaagaaagc atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga
     9001 gtataattat ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat
     9061 atcaccaatt attgattcga caggtctatg tttattagat gaattcttgg cggatatcag
     9121 tctatttata atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa
     9181 gttcagaaaa tttcgtatga aaaatgataa acaaaaatta ttggcaaatg agatttggag
     9241 tagtttagaa gtcgaagaaa gtaaacaaga acttttgttg aaatggatac tttttcttgt
     9301 aaagaagttc tatgtgaaaa taccagaaac ccattgtctg atagttatgc acggtaaaat
     9361 aacagcttct gcgatcgcaa gggaaacaaa taagttgctg aatacatatg tttatgaggc
     9421 tttcgatatg ccaattgaag gtgagactag tgacttaatt aacctaatca acaatttttg
     9481 taaaagtata gatacgacga atggactcat tctgctagtt gatatgggat cattggaaca
     9541 aatgtatgaa aaaattgaat ccaatgtaat aggtgatttg gtaattttaa acaatgtttc
     9601 tacagcttta gcgattgaat gtgggattca agtttgccaa aaaaagccaa ttagtcattt
     9661 ttaccagatg gattttgatt cgtttcaagt taaagttcag tattataaag gattatcaca
     9721 aaagaaaaat gtaatagtat cttgtctctc tggtgagggg atttctgaaa aagttaagga
     9781 tattttaaaa cggtatctcg ataatcaagt ggagattttg atttttgatt ttaacgcatt
     9841 aaagaaaata gcaaaggaaa aaga
//
LOCUS       DAZUJO010000097         9809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000097 DAZUJO010000000
VERSION     DAZUJO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.101"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1358)
                     /locus_tag="ACG8OL_002497"
     CDS             complement(69..1358)
                     /locus_tag="ACG8OL_002497"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS4818434.1"
                     /translation="MKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKD
                     TAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLPF
                     YNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQFVT
                     GFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQKET
                     TFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHFIKQ
                     DVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKLISD
                     SEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQPKG
                     ILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(1495..2010)
                     /locus_tag="ACG8OL_002498"
     CDS             complement(1495..2010)
                     /locus_tag="ACG8OL_002498"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS4818435.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(2083..2997)
                     /locus_tag="ACG8OL_002499"
                     /pseudo
     CDS             complement(2083..2997)
                     /locus_tag="ACG8OL_002499"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(2994..5321)
                     /locus_tag="ACG8OL_002500"
     CDS             complement(2994..5321)
                     /locus_tag="ACG8OL_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS4818436.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            5930..6076
                     /locus_tag="ACG8OL_002501"
                     /pseudo
     CDS             5930..6076
                     /locus_tag="ACG8OL_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(6164..6457)
                     /locus_tag="ACG8OL_002502"
     CDS             complement(6164..6457)
                     /locus_tag="ACG8OL_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS4818437.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            6494..6679
                     /locus_tag="ACG8OL_002503"
     CDS             6494..6679
                     /locus_tag="ACG8OL_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818438.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(6860..8038)
                     /locus_tag="ACG8OL_002504"
     CDS             complement(6860..8038)
                     /locus_tag="ACG8OL_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4818439.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(8142..8456)
                     /locus_tag="ACG8OL_002505"
     CDS             complement(8142..8456)
                     /locus_tag="ACG8OL_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818440.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            8563..8778
                     /locus_tag="ACG8OL_002506"
     CDS             8563..8778
                     /locus_tag="ACG8OL_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818441.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(9078..9320)
                     /locus_tag="ACG8OL_002507"
     CDS             complement(9078..9320)
                     /locus_tag="ACG8OL_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818442.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(9307..9735)
                     /locus_tag="ACG8OL_002508"
     CDS             complement(9307..9735)
                     /locus_tag="ACG8OL_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS4818443.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         2775 a         1898 c         1690 g         3446 t
ORIGIN      
        1 tgtcacaacc tttctcccca tttgaatcac tatcgtttat ttatcgtatg atttttgttt
       61 ccaatagttt aagcttctgt ttcttgatgg cgttttttga agtagatcac tgctcctcca
      121 actaaagcag tacctaccaa gatgaatacg atgatcccca taccaccagt tgaaggcaag
      181 attcctttag gttggttatc gatcgttgtt tcatcagaag ttgttggatc atcatctact
      241 gtaaattcag tgtacgcagc ttcattctta ggtaatgcgt agccttctgg tgcttttgtt
      301 tcttgaaggc agtatgcccc ttgtttcagc ccttcgattg aaaatttccc ttctgaatca
      361 gaaatcaatt tcacttcttc ccagtcagaa cctgtgactt catctgacca agcatatggg
      421 tattctcctg aggcaagatt tgctattttt ttcattaatt taacagttgg atcttttgct
      481 cttagaatag aaaattctgc atcggctaat ttttcttgcg tgttcacatc ttgtttgatg
      541 aagtggaaat caccgtagtt ttggacattt tctacagtaa tcgtgttatc cgtagttgta
      601 ttttcccgat tgactacacc gtttggattc ccaagattgg cttcatctgc gccatggatg
      661 ataccggttg tttgttgcaa ttttccgttt tttactataa aagtagtttc tttttgtgga
      721 ttggttaatt gataaccatc tggtgctttt gtttcaacta aagtataatt gccgtctatt
      781 aatccattga cactgatgga tccttcagta tcagttgtga atgtcaacgc tgactcagca
      841 ggatcataga gagcgacacc agtgttttca tcaaaaccgg tcacgaattg attctctgaa
      901 tttttgatct ggaattgcgc ccctgctaac ttatctaccc ctgaaaagtt cccagctttc
      961 tggattttga tattgctttc tttgatgaca tttttagcat ataggtgaat cgtgtctaaa
     1021 ggtttgtcag ggtctgatga attcgggtta taaaagggta agcttaacac gatatttggc
     1081 gacgtttcga caccgggctt tggtgtttct acaaagacgt atacagcatc tttcccatca
     1141 gatttacctg gaagagtcag gttagctaat ccttcaccag aagtcgttac acttgcgaag
     1201 ggtgtagcgt ctgctggaac tgctgtgtct ttacttggat agtacgtagc agtgatgtca
     1261 tatacgttga attcaacgcc gttcaacggt tcgctattcg gaaatgtcaa ttccgttccg
     1321 ttgtttattt gattttctgg tgctgaagtg aatttcaatt tatgcaaatt aaccgtaaca
     1381 ttttctggtg taggtgatac ggcctagaaa cctctgcagt tcctaggccg agaacgaaag
     1441 gaagcagcat gatagcagtt cctaaaaacg tccatatttt tgttttgatg tttttcattt
     1501 tatttccctc ctattgtttc ctttttgttg ttttttataa aaccaagctg tccctgttga
     1561 aaggactagc aacaacccta aaattgagaa acctccaagc atctcttcgc ttgtttttgg
     1621 aaagcttcct ccattttttt catttgatgg accatttgtc tgcatctgaa ctggcgtttt
     1681 tccattattc gtcggatttg cttttggttc gttgatgatt tccaatggta taggatgaag
     1741 cggatcataa ctgccttttt caacttcaaa gagaatggtt gattgactca atatatatcc
     1801 tttaggcggt ttaatctcca ctagttcgta acttccaaaa gacaatccat ggacagaaaa
     1861 tgttccatct ttactggaag taaaaatcgt tgctcttgaa tcatctttgg ctacccaatg
     1921 atttatcccg ttttcttgcg ctaagtattg gtcatttttt ttgactagaa attgagcatc
     1981 tgctagtttt tgtttcgttt gccgatccac tttgacaaat tgtttgcctc ctgtttggat
     2041 ggcaagatgt gtttctactg tttgattgtc agtcgtcaaa cgtacatcgt tatcaaatgt
     2101 cgttttttca ctgttttctt tcaatgtcat ttggtaacgg atcgttaatt ttttcccagc
     2161 aaacgaacta agatcttttc cagaatctga taagacaaaa ccatgttttg ttttttgtgt
     2221 gtgaaatgta tgaatcttgt ctccaccaat caaaaaatcg atcgtttctg gttctagcca
     2281 taactgagga tcagctgtat ctttgatcgt ataggttcct atctcgttga tgttctcagg
     2341 aattgttacg gtgatgagat aaggaacttt atccccatct gcaaaatctg tctgcttatt
     2401 ctctactgtt tttgttaatg tgagtgcagc aggtattttt tgttcgctct ttggatacaa
     2461 gtggatggtc gtcaatttat gattagatga atctgtcaca ggaagcacaa cgacaaatgg
     2521 tgctgatttt tcttttactt aatctgaagt ctttatttcc gcaaatcggt aaacagcgtc
     2581 acgaccttgc tcatctttgt ccgatgcact aaaagaagca atgccctctt cctcgtctac
     2641 tgtttttgtc acattttctg ccagtatttt ttcactatca cttttttgtg ctagttttct
     2701 ttgtgcgtct tccacactac ttccttctga ccgcagcttg taaaattcat ccgagacatc
     2761 atatatagcg aaagtaacgc cgttcaaccc ttttactcct tgaaagttat ccgggatatt
     2821 tattccgtcg ttttgactga tacttggcat cttatcgctt tcgaacattc gattatgcaa
     2881 agtaaatgta ataggtgatt ctctagctga cacagtcgga gaacctataa aaaagaccag
     2941 taggataaaa aagagaacag caaatatatc cacacgtaca ttttttgttt ttttcatcgc
     3001 tgttcacctg cttttttctt acgatggata aaataaaaga agccagaaaa aataaagatc
     3061 agtcctaaaa agacaaaggg cagtagacct atcccacctg tcgaaggaag caagagagtc
     3121 ggattgatcg tcaacttagc ggtatctgtt gcgataatcg tataattatt gtttacactt
     3181 ccttcgccta aactaatcgt tactatttgt gatccaatat ctgtccggct gttaactgtt
     3241 agggtataat ccaatgattc gcctaagaca acaccggtaa cgtggagatt tccaaccgaa
     3301 atcgagtgac tttgggcatc ataattaaag gcagcgtcat cgacagtaat cactgcgtta
     3361 gctttataga tggtataagt tcctgatgtg ttggctatat cagtaaatga caacttggga
     3421 tttgctgctt tgactttgtc tattccgcta ttcgttagtt taattgtata gctgccagca
     3481 ttaaccatat tttttgctga ttgtgcatct gctgtgtaat ccatactgct aagtgccact
     3541 tgtactggag taagcgaact agtgacaccc tctgctagct tgacatggat tgacgggaca
     3601 cctgcttttc catcatatgt cttactgcca ctgagctcta ccgcaccgct gatttgctta
     3661 atcgtataga catcagatgt ctgcacgata gtaaataaat agtttgttct ggcagctagt
     3721 ttagctagtc cgctttttgt caattgccaa gacgtatagg ttccaatttc agtaggcgca
     3781 gttacttcaa ctcccttttc attcatcgct ttgaaatcag ttggattcca tgtgatttga
     3841 tcaatactga gatcattgtt taaaagggat gttgattctg ttttatcatt aaaggcatat
     3901 ttaacgatcg gtacaacaga aagaccatca tagtacttac tgccatttgc attgattgat
     3961 actgttgcat ttgtcttata atataggatg acttcagaaa tctgttttcc atttgcgtcc
     4021 tttacgatta catttccatt tgtatctaaa ctagctaaaa cactcattgc tgattggttc
     4081 tttgtttctg tcgctgaaac agctgtaaag gtatatccgt tgatagtagg cgtattagtc
     4141 gtcaggtctt gtgcagcact tccgtaagta agattcatta ttttagaagg agcaatcatc
     4201 tgtccaaaat agtcttgata cttaatagtg attggtgttt gtttgcctgt ataggtcacc
     4261 gtatcgacgt tactttctag ggctgtgaaa gaaccgtttg tttgtgcaaa agaatagccc
     4321 gttactgctg ttggagtgat aaccactttg tcacccacat ggattccaga taatgtagtc
     4381 ccgtacgttc cgatttttgc tgctccacca ttgactgtat aagaaaaagt tcctgtccga
     4441 ttcgcattga cctgaaccgt tgctgcttga taggttggaa aatttgaaac atttccctta
     4501 ccatcaacga tctgcccatt tttgatcgag taccccgtga atggaatatc atttgctaac
     4561 tgtataccga ttgaaaaagt actcatacct cctccggcag gaagccctcc ccatgttgag
     4621 ggtgtaaaag aataactttt atccgcattg ggtgtaactg aatcgatcgt atttccagcg
     4681 gtagccaccc ccatatagat actttttggg aatgtgatag tagtaccatt tactgtggag
     4741 ccaataaaca gattatggat atctggacgg ttatcaataa aagcgattgc tttggttacg
     4801 ccttgtaacg cagttttatc cgcactcatc gatccgttaa ccgcaacaat gactccatct
     4861 ttggcatata tcactgtata tccatctggt atggtatgtc cgttgaaatt cgcagtggcc
     4921 gcaacagtac taaatgtctg gcttctacct gtttctgaat aagctttctc ccctgctgct
     4981 tttgctgctg tgatttcttt ttctgtcgga tcaatcagtt gagtcgagga tgtggttgag
     5041 gttgtctcgt tgttgctaga ctgattggtg ctttctatgg actgtatttc agaatagggt
     5101 ggactatttg tctcctctgt cgtttggtta gcaaaatttg acgtttctgt ataaacgttt
     5161 acataaatac ttgataagct ccctaagaaa gtaaaaatca agatgatcga aatcataatg
     5221 ggatgtaagt attttctttt agatacagct ttgagtctag aaacaaaatg tttttgacat
     5281 atacgttgga ttttattttg agttttctta atcattttca tccctcctct caataaaaaa
     5341 tcatcaaata atcctttaca cgttattcta aatttaattt ttattatttt attgatgtat
     5401 agaaagtttg gggctttttt tctcttttta ttctaaggtt attttagtct tgtcctttaa
     5461 tacaaagatt ttatttttaa ataaaaaata ctcttttttt acgattattg aaaaaaaaag
     5521 ataaaacttg attaattgaa gtattgctta aatttggaag ctatgaggga agaataaaag
     5581 agttaggacg ggattatcta ctatttttct ttgttttaaa tatttgttat ctttgaacat
     5641 aaaagagaaa tgtgtcgact gcagtgttgt gtccctcatc acgaataaac ggtgttcaat
     5701 tagctgatct tgtattcact taaaaaatgg taaagtacga gttgctattc cccattttca
     5761 attttctaac gctcaggaca aaatactgtt ttcacaacct cattggaata aaagcatata
     5821 tattgtttca agaacgaaat cttgttatct ttttaaaggg catctgtcca aaatctaatt
     5881 ttaggaggta cgttttggta tttattcgta caaataaaag agaattatct ttattcatgt
     5941 aacgcatcca attgattttt tcatgtacgc tcctggaacg attttactga cgattgctta
     6001 tttaactgcg aatcgctcat tagcttttat catagctatc catattttga ataacgttct
     6061 tggctttgta ttatagatac agatgacaaa aaaagttcaa gtatgcggtg tagaaccaat
     6121 atcgcatact tgaacttttt tcaatgagta taaattaaaa cttctaattt tcaattccca
     6181 tcatctgata tgcccgaccg tctgaacttc ttttgaaaaa gccaaatgtg attagataac
     6241 gtctttgttc gacataatcg ccactagaaa atacgttcag cagtatcgaa ttaacctctg
     6301 tttctgtata agtcactcca tggtcaaagc gtttcaagac ttctttcaat aggatgtatt
     6361 tcttttttgt tgaacttggt acttgtttta cttttccatc attcatgaat gtgtctaaaa
     6421 ctttcatctt ttcttgctcc gtataagtat aaaccatctt ctttctcctt ttgattctaa
     6481 gaatactcca tttatgttag aatattctgg attttttatt aaagcggtta ctcttaagaa
     6541 agatattatc atagtgctct tttttttact actacttata ggtagattta ttctaatcca
     6601 ttattctccc ctaccaataa ttaaagaaaa aactggatca gggtgttcaa attacatgct
     6661 aagaaaaaag aacccttgaa taccaagggt tctttctcag tctgataaca atgattaacg
     6721 acggatttct ttgatacttg tgaaaagtcg ttaaagtatt gatataaaag gatttttatt
     6781 acctctgact acatattgac taatttgtta aaaaatcgtt tttcaagaat cctgtaatac
     6841 cagtgctttc accgtttttt taactctcta aagaactaaa agcaatcaga ggttttacct
     6901 tttcatttga cgttaagtgg cgataaattt tcgaagttgt ttggtcagta tggcctaagc
     6961 gatctttcaa gacttcccag ctacagtgtt ccgctactaa gcttgccatt gtatgacgag
     7021 ctaaatgagt atgaaatggc ttattaattc ctgagcgatt cacgcaccgt ttgacgtgat
     7081 catttacata ctctcgacga taaggaacac cacgtttact agaatcaaga tacacaaaca
     7141 aatatgtttc ttctataata aacctgtaat gttgcctcat ctcacccaaa gccttaatca
     7201 actgttgtag cttttccata actagaggag ttactagaac tcgtctagca gaactaactg
     7261 tcttagtagt ttcaattcga aaatccttac tcttaccttt aactgccgtt tgcttataga
     7321 acagaatttc atttcttttg aaatctatat ctccaggctg tagagcaagc gcctcgctga
     7381 ctcggcagcc agtgtaaaat agaacatgga aaagtacaga catcggtaat tcctcatgaa
     7441 caatgccata attcaaaaaa gttctcaatt catcgacagt aagaaacttg tcctcaatat
     7501 tatttatggc ttcttttaaa tcagttaccg ttaactgata ttttggcatt ctcacagact
     7561 tcattggatt ttcacttatc aaattctttc tttgacaata tttaaaaatc atatttagaa
     7621 cttgaataag tgattgtaaa ctatttttgg catagccaac atttgttcca ttaacgccac
     7681 attcctttaa ggtaaaaagc aattcttgaa ggcactctgg tgtcaaatca gacaactttt
     7741 tatcaccaat ccatcgaaca ataattttta attgttcctc tcgcttatca taggttattt
     7801 ccttttgttc atttaacgaa cggtaatatt ggaaccattg ctcacctaca acactaaact
     7861 tgtcattgct tgcaacaatt tttttcttaa gcctttgttg tttctccatt tccagataag
     7921 cgagttgcgc ctcacgtttt gaattaaagc cacgtcttgt tgttcgatat gcttgaaagt
     7981 accatgtgat cctaccagta gaactgattt gcttcttgaa tcgatctgtt ttttccattt
     8041 attgattcct cctattttga aggaacccaa caagcagaaa ccctttggaa atctttctat
     8101 ctatgtcatc attgtaccgc atcttagaaa gaaaatgtaa cttataaaga tcgaagtagt
     8161 cgcaattttt ctcgatagtc attcgaatca ctctttctca tccattctaa aacatcatca
     8221 cgaaaatagt gtcctcgaat cccgtaccaa cagggaggaa actcaccaag ttttactaaa
     8281 cggttcagtg tactatcact tatctgaaat agtcgcttta aatcagtatt tgttaatacc
     8341 agaggtaaat gactgactgc aagcaatttt tccgtgtaat cttccagttt tttttgtaaa
     8401 attgaataga actctatttc cagagaatta acatttgttt taacttccaa ctccatcacc
     8461 cttctctctc aaaatcaaaa cgaccaccac ggtacccctt aaaattgtaa aaaaacactc
     8521 cagtactcca tcaaaacgga ctagaaaaga ggaattttta atatgaaaca aggaattgca
     8581 gacataaaaa taattaaaga aattcttgaa aaatctacgg ctaatgcaat agcattcgga
     8641 acaggaatca acttgagtac agtaaaaaaa cttaaaagtg gagagagagc tgaagaaaaa
     8701 ttaaatcttg ccgatgctat taaaatcaca gaatttggca tgaaaaatat gccaaccaaa
     8761 atcgaaatat ggaaatagta gtttttctga tctatcagct gttttgccaa agcacatagg
     8821 aatctcacct ccccccagta atggcgggcc gatttggtta cggaagtatc attatcgtag
     8881 tcgcgattct catattaaaa tttgtcgctt aaaagctaac cttgtcatgg cgtaactaga
     8941 gaatcaaaat ctacatgtac tgatagattt gctattcatt tgtcaaagag cgctaggcta
     9001 ttttcagcct atggaaatcc tttattttga cccgataaaa aacttccata gaatgaaaag
     9061 gaaagggaaa aaatacttta acgagttgta tcaaattcaa gtatcttctc aatcaatttt
     9121 gtttccagtt gtctacgcat atattcatct acatacataa ccattccttt ttcttcttca
     9181 taaactggtt tcatggctaa ccgtgaaata taattttgat attttgacag aacgatggac
     9241 atggcaattg aatcaccaga agctgcggct ttgattgtcg ttagtggtat gaatggtata
     9301 tatcgtttat tttgcttcat atttgacacc caaatacttt cctagctctt ccaaagccat
     9361 ttgtcgttgg taccaaacac tgccgagact tttcccaata atatcgccaa tttcacgatc
     9421 attaagaccg atgaagaagg acataaaaat gatttcttgt ctttgactag gtaatgctgc
     9481 aatcgcattt gccaactcct cattggaaat aaagattttc ataccaagga caataaaagt
     9541 accactacta ttcaaatttg gttccaaata atagaactct gtacctaacc ctttttgttc
     9601 caatatttcc attgatattt gccgttggcg aaatctagcg tacttctttt gaatatttaa
     9661 cgcctcattg cagactactt tcttacaaaa actgtcaaac gtatgttgaa tgtgggtttc
     9721 ctctgagtcg cccataatat tctccccctc aaaaaattca ttcaactcgt tttacttcta
     9781 tccctttcca ttattactac aatctagga
//
LOCUS       DAZUJO010000098         9559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000098 DAZUJO010000000
VERSION     DAZUJO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.102"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..1087)
                     /locus_tag="ACG8OL_002509"
     CDS             complement(245..1087)
                     /locus_tag="ACG8OL_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4818444.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(1124..2590)
                     /locus_tag="ACG8OL_002510"
     CDS             complement(1124..2590)
                     /locus_tag="ACG8OL_002510"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4818445.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            complement(2646..4490)
                     /locus_tag="ACG8OL_002511"
     CDS             complement(2646..4490)
                     /locus_tag="ACG8OL_002511"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS4818446.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            5092..6042
                     /locus_tag="ACG8OL_002512"
     CDS             5092..6042
                     /locus_tag="ACG8OL_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS4818447.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKKQIIENTLFSESKNFSKAIDVSPKVTSVA"
     gene            complement(6129..6215)
                     /locus_tag="ACG8OL_002513"
                     /pseudo
     CDS             complement(6129..6215)
                     /locus_tag="ACG8OL_002513"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(6259..6720)
                     /locus_tag="ACG8OL_002514"
     CDS             complement(6259..6720)
                     /locus_tag="ACG8OL_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFS4818448.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            complement(6980..7528)
                     /locus_tag="ACG8OL_002515"
     CDS             complement(6980..7528)
                     /locus_tag="ACG8OL_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818449.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(7529..8386)
                     /locus_tag="ACG8OL_002516"
     CDS             complement(7529..8386)
                     /locus_tag="ACG8OL_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS4818450.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(8672..8959)
                     /locus_tag="ACG8OL_002517"
     CDS             complement(8672..8959)
                     /locus_tag="ACG8OL_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4818451.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(8949..9278)
                     /locus_tag="ACG8OL_002518"
     CDS             complement(8949..9278)
                     /locus_tag="ACG8OL_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4818452.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(9355..9501)
                     /locus_tag="ACG8OL_002519"
                     /pseudo
     CDS             complement(9355..9501)
                     /locus_tag="ACG8OL_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         2888 a         1931 c         1460 g         3280 t
ORIGIN      
        1 attcgtcatg gcaacgcatt gattttcaac taaaaaagat caagaggaac tgatccactt
       61 gatctccaat tattctaaga tttctcttta ccaacatcat ttgacaaaga gccgagattt
      121 tttattatct tacgttaatc ctttttaaat gtctcttata gataacaaac agctggaaat
      181 caccttgttt ttactataat ccatctctaa agattttaaa agcatcaata gtattactga
      241 gcgctcaccc attagcatgt ttcatcaacc gattgatgtg gacacctagg tatacaactt
      301 catcgttcgg aattgtttta tgataaactt tttcaatgta gtctttgatc ttagctgcgc
      361 tttccatggc agacgggtat aagatccaca tttgattgta gaattcttcc tcaccatcat
      421 ccaacaaagc atcagaataa tatctttcta caaagaaacg tacatgtgta atgaagcggg
      481 cataatggat cgaattagta tcgatatctt tctgcaacga ataccggacc aaattgacga
      541 tcccgccgat catttttgca tacttgaatc cagcgccgct atcaggatct tccgattgtg
      601 cattgatcaa atggaaagca atgtttgagg cttcctcttt aggaagatca attccatatt
      661 gctcgttcaa aagggtgagt gcgtacttcc caatttgaaa ttctttggga tagtagattt
      721 ttatttccca gtacaaacgg tttgcaatat ttaccccttt ttgtgcgcgt tcaactgcaa
      781 aatgcaggtg gtctgttaag gttaaataga ctccaggatt cagtttttta tctaatcctt
      841 tttctgcttc aaaaacgata gtacgagtga tttcaaaaaa ttcaataggt atttcttcaa
      901 ctaactctaa tagttgtgaa gatttctttt cttcaaaagg cagaaatatt ttatctactt
      961 tatctcctga tatttcatcc ccagctttct gtccaaagcc tatcccttta cctaatgcaa
     1021 tcatttcttt gccattttct tcaacaagta ccacacttga attcaatact ttttttattg
     1081 aaatcactca ttatccccct atcgctgctt aaaaagatcg agactactcg aataaggatg
     1141 caccgtttgt ttcgatcact tctttgtacc agttgaacga atcctttttc aagcggttat
     1201 atgtcccatt tccttcatcg tctgcatcca cataaataaa tccatatcgt ttagacattt
     1261 gagaagttcc tgcactaact aaatcaattg gtccccagct agtataagct atcaaatcta
     1321 caccttcatc gattgcttct ttcatttgtt ggaagtgtgc tttaaagtag tcgatgcggt
     1381 acggatcatg gattttacca tctactacct gatcgactgc tcccatacca ttttccacaa
     1441 tcatcaacgg tacttggtaa cgatcataaa gttcgatcaa cgaaattttc aaaccttttg
     1501 gatcgatttg ccagccccaa tctgttgaag gcaaatgagg attcttaacc cctaaaacag
     1561 tatttcccgg tgtgcgttct gcgtttggat cagctgattc tgccatggac atgtaatagc
     1621 tgaaagaaac aaaatctacg gtatgtttct ttaaaatttc tcgatcttca tcttccattt
     1681 taatctgaat tccccgattt tccaaatcct tttcgattag agcaggatat tctcccttaa
     1741 cttgaacatc tgcataaaaa ttattttcta agttcttttt caaggtcagc tcaacgtctt
     1801 ctggtgcgca agttaatgga taagtcatca atttcgttaa catacaacca acttggcttc
     1861 ctggaatttt ttcatgagca tcttttgtca ctaacgcact cgcaacaaat tgatgatgca
     1921 atgcttgata gatatcttga gcttcttgac cttctgcgct tttctcttgg ataattccag
     1981 ctgttgtaaa tggatgacgg tggatgctgt ctatctcatt aaatgtcagc caatatttta
     2041 cgtatttgcc gaagcgatca aaacaaacgt tagcgaagcg aacaaaatca tcaattactc
     2101 gacgctccac ccagccatta tatttaactg ctaaagctaa tggcatttca taatgagata
     2161 gtgtcacaat cggctcaatt cctaaacgtt gcatttcttt aaagacgttt tcataaaatg
     2221 caaccccttt ttcattcggt tccagctctt ctcctgtagg gaacaagcga gtccaagcaa
     2281 tagagaggcg caaggtttta aagcccattt cggcaaataa agctaaatct tctttatagt
     2341 gatgataaaa atcaacgccc cttctttttg ggtaccattt atcagatttg tcttcactag
     2401 cttcttttat agactgcagg gtgacatgca tatgagcagc aaaatctttg ttgtctaaat
     2461 ttggtttata agtagccata tccgctactg aaagaccttt accgtctaca ttccaagcac
     2521 cttctacttg atttgctgct atcgcgccgc cccataaaaa tccttttgga aaacttgttg
     2581 tgtttgtcat tattctattc tcctctattc cttcattcaa tatatttttt ttcactatct
     2641 tgtgtttaag ctatcggact gatagctaag agatcactat cattggttac caatttttct
     2701 aggttaaatg aacggatagt gtaattatcc ttattggtta ccacacagat aactgtgggg
     2761 tcgaatcctg cttctttgat taaatcaata tcaaatgcaa tcagcttttg tcctttggct
     2821 actttttcac cagaacgaac aaatgcctca aacccttttc cttctaactg aacagtatca
     2881 ataccaatat gaaaaagaag ttctgcacca ttagccgttt ttaaaccaat agcatgcttc
     2941 gtttcaaata ccattgtgat ttctccatct tcaggagcta ctaatatccc ttcttcagga
     3001 atgattgcta ctccatcacc taccatacca ctagaaaaaa cagtgtcttt tacttctgat
     3061 agtgagatta cttttccatt cacgggactt gaaagaacag catctgaaac aacggccgtt
     3121 tccatcttag tttcaacttc attgacaact tctttatctt tataaagaac caacgctgca
     3181 gcaaaagcaa ccacaaatga aatccccgat actgcaaatg cccaaaccag attcattggg
     3241 tcagctttgt cagcgaacat cgtgatactt gcaatcccag gacccactaa agcaaaggct
     3301 tgaagtgcaa cgattcctgc aaatgctccc ccgatcaggc tagaaaacat aacggcatat
     3361 aaaacacgtt tgtgcagaat cgttaccccg taaagagctg gttcagtaat tccgaataat
     3421 gcagatatac ccgcagaaac agccgtagat ttcaattttt tgtcttttgt tttgatactg
     3481 acagcaaagc atgcgccagc ttctgaaata ttgtgtgcca atgaagccgg gagataaaga
     3541 agttctgcac ccaattcact catggatgaa acagcgtaag ggatcattgc tttatgcata
     3601 cccgttacaa ccattaatgg cagtacagct gctaagactc cggttgcaac ccatccaaat
     3661 tgattgtaca aagacacaat gatagaagag aaagctgttc ctacattata acctaatggt
     3721 cctagaagca acaaagcaat aggaactgta atcaataagc tcatcatagg tacaaagaaa
     3781 atacgaattg gttttggtga aacttttgta aaccattttt caagttgagc ataaaataaa
     3841 acagttaaga tggctgggaa tacttgagat gcatacgcta tattatccaa tccaaaagat
     3901 aagaatgtag caccttccgc caacatagct gtcatttcag gaaggatcaa tgcaccgaca
     3961 gcagatactg caaccaattg attgactttc aatttattag ccgtagtcat tgccaccaga
     4021 ataggcaaga aataaagcgg tgccccgccg accaaattta aaatctgata ggtttgagat
     4081 tggtcactaa tcagccctaa gatagaagct agtagcaaca aggacttcaa tactcctcca
     4141 cctgcaatcg caggaattaa aggttggaaa atagaaataa taaaatctaa tagaactgaa
     4201 cctaattttt gtttttgttt tggtgcttct tcagtatttg aaacattgtc acctaataac
     4261 gctttcactt catcatacac ttcaacaaca tcgtttccaa tgattacttg gcattgcaca
     4321 ttttggcgaa cgcccataac gccatccact ttttctaact ggctcatatt taccttgctg
     4381 ttgtctttta acgtaaaacg taaacgagtt gagcaatgct ccaaatggga tatgttttct
     4441 tccccaccga tgtactgcaa tacttcttct gctgtttgtt tgtaattcat ggtgtttccc
     4501 tcttttcttt tttgagtgat gaacttttga gtcgaagtgg ccatcctcaa cacaaaaaac
     4561 aggcagaaac taaataaatt gacaacaagg caaacgtgta ttgaaacgca tacattgtta
     4621 ttcaacttat ttaggtcctg cctgcattac cagtcacatg cctgtttaag ttatattcaa
     4681 tttaacatat tagaatgtaa acgtaaataa taaaatgatt ttttaatcat tttttcattt
     4741 tattcgttca atatagtttt cgtgttctaa aagttttgtc ttttttctaa gaatatcttc
     4801 caacatatat aactcttatt ctcttctatc ttctagcaga tgttctgtct ttttaagacc
     4861 tagtataaaa agaatgggtt cattttttct ttcttgcagg atgaggtttt ttatataaac
     4921 tttatttgat ataaagaaaa ggcattcata attgtgctat actactaaaa aatgataata
     4981 cagtgaatta gattagcgct ttattaaatt aagaggacag catatcgata ttgcttcctt
     5041 ttttttgcta tttatttaag ggatagaagt ttagaaagga ctgtttataa gatgaaaaat
     5101 gatatagtga cttatgcaaa cacaaaatgt gaagaaattg gcatcagtaa tttagaagag
     5161 tgcttttttg acacaaatga cctgtcgcaa tttttaagaa cgggaagaac aaatgccagt
     5221 aaacttgcca atgacttagt tgcaaagaac gttctattta aaatcaatac tcgtccagtg
     5281 ttatttgcca tcaaagagcg ctttgaagag cactttttga ttccggtaaa agatgtatac
     5341 gaaagtatat ctgaattaaa aacagacttg gagcccaatt ctacagattc cattctacaa
     5401 aagatgattg ggtatgatat gagcttatcc gatgcggtgg aacaaatgaa aactgctgtc
     5461 ttctatcctg gaagagggct tcctatgatg atcactggag atactggagt tggaaaaagt
     5521 ttttttgttc atactgctta tcagtttatg aaaaaagtga acgttattca tgagaatgct
     5581 ccttttaaag tacttaattg tgcgcaatac cataataatc ctgaattatt gtcggggata
     5641 ctatttggct atgttaaagg tgcttttacc ggcgcagatg aagatcgaaa tggattgttg
     5701 caagatgcag acggtggagt actgttttta gacgaagtac acagattgcc tcctgaaggg
     5761 caagagaaac tatttgttta tatggatggc ggaacttatt ctagagtcgg tgaaactatc
     5821 catcacgcct ccaatgtacg tttagttttt gcgactacag aaggcaaaga ggattttctg
     5881 gaaaccttta tgcgaagaat ccctatacat atccatatcc caaatattga ggaacgaggt
     5941 attttagaaa aaaaacaaat tatcgaaaat actctcttta gcgaaagcaa aaactttagc
     6001 aaggctattg atgtttctcc taaagtcact agtgttgcat aaatatagtc agaatattct
     6061 gaattattat tttcttgaaa aaagtaaaaa aaatcgtatc ccaaactttc atctacattt
     6121 ccagaaagat cttcatgttg ataatcaatt cctgaatcaa tgaccgctac cttcacatcc
     6181 ttagaaccct tggtttcttc ccaagcggat ggcatgctaa tctctcttaa attagcttgt
     6241 ttactccaat taggatcgtt atcgatttcc taaagaatca gttgtatttg gctcctcaac
     6301 atcattcaac ggctctagtt caaccacata gtttggctct gcataattga ccatatcaga
     6361 agcattaaat gttcttactg cctcttccac actaatttct tcgttcaatt tcactaataa
     6421 tacttgatct cgcttattat aatcgctttc atttgtccta agagtcaaat ctttttccac
     6481 cataactttt acacccaata aattttgcgg tttatttttc tctgttgaaa ttctttgtgt
     6541 agcctttaaa ctgacaatta gctcattggg tacatactct atttgagagt catctaattg
     6601 gctcataatc ctttcttttt ggttagtatc tgcagttact tgtttagtat gagccatgaa
     6661 cccaaacata ctcaccccaa caaccaatcc taaatatatt ttctttttaa actttttcat
     6721 ctacatctcc tcctaatagt tttattttag agttagctct attacttatt tactaactca
     6781 acaacagatt atcatacact tattaatgaa atcacaatat ctcattaaaa cacaccctaa
     6841 aatcataacc gttcctatgc tcattttttg gattttttaa tcacgtaacc agaaaaataa
     6901 atcccctcta tttcacgcaa atcaaagaag aactaagtaa ctaccgttta aaggtagtta
     6961 cttagttctt ctttgattat tatctaactt tattattcat tccccaccct ttgcgcagtg
     7021 agtcttcggt atcttgtttt atctgactgg tagttcttat tatttctcca cgatcatcta
     7081 attctggaac aattggatat ttttctctga acaagtctag ttctttcacc acgtcattaa
     7141 acaaatcttg tttatcttct tgcgtaagat tctgtttaat atactcatct aaagcttttc
     7201 taagcaattc atctcttgtc atattagtct catacttttc tactaaaact ctggaagtcg
     7261 catttaacaa agtcgacagt tttaaaggat gcttggtgga ataaacctta tcaaactttc
     7321 tgattggtgc gccaactttt tttgttcttc ctctactctg tctttcaaca ttggaggaag
     7381 tttttatttc ttgaacttgg tgcacttcta attgcttatt ttcagatggt gtaattgctt
     7441 gctgcttttt ctttgcttct gcttcttttt ttattttggc gagtcctttg ttgttcccaa
     7501 aaatatcaag tcctttgatg tcagccaatt actccacctc cgcataccaa ttctcgtgtt
     7561 cttccaattc tttcaaaata ctgataaata attcgtggct tttgcgatcc cattgtttta
     7621 agagaccttt ctttgtcttc tccatcgtaa tacctgatcc gtcgtatcgt tttaagcgcg
     7681 catttttgag tacaacattt ttaatcaaat gttccccata aatctcttgg gcttgtttat
     7741 acatttcctg atctactgcg tcaccgcttt ccaacatgaa aggaataaca ccagcaacat
     7801 ataaatttgt atcaaattct ttcacaaaaa agtccatata ctcaagatat tgcatcgcat
     7861 ttctcatcga ttttacttgg gtctgtaaga cgacgattgc ccaatccgca atgtacatcg
     7921 cggatttact atatacagaa atagaaggtg ggacatctaa atagatgacg tcaaactcat
     7981 caaataatgg ttctagcttt tctttcatat atttgaattg ggataatgaa tcattatatt
     8041 ggtctttgat ccaatcatca tacatttcaa aggacatgtc tgctggaagt atatagaggt
     8101 tattgattac tttatagaca gcctctttag gtggaagatt gtttatcaac acgtcagtca
     8161 cagtattttc gtaaacaatc ggttcacctt ctccgcctcc ttcttcgtta gtgaagttca
     8221 tatgcgttaa gttcatcaca tcagaagtat ttccttgcat atccaagtca attatcaagg
     8281 ttctttttcc ttcattaact gctgattcaa accctaacat ttgcgcgcac gtacttttac
     8341 ctacgccacc tttaaaatta ccgattacat atttcttcgc taccacgttc ctacctcttt
     8401 tcttttagca atattcattt gatactatta taacatattt atttttagtt actaccaaaa
     8461 aaaagatata tatgtactac cttataatta ataagtaaaa ggtagtacat aaataaggaa
     8521 aagctgaaat atattgattt atagctttcc tcaaagtaat gacgttattt aatttatcga
     8581 gaagcagaga atactcgcga ctttagtcat gtagacgaat cagattttcg ataaaaaaga
     8641 agccgtcctt cttaagaacg acaacaaatc actagattaa ttttttcaac tgttcataaa
     8701 agttttcatg cgttccacat aacaatactg gaataacctt gttttctata atctcgtaag
     8761 ctactctata tgttgtgccc gcatatttga acccactaga ccaaatacct gataaatccc
     8821 cagttttttg ttcaccctta tatggatcaa tcgcaatttc atcataaatt aaattcaaaa
     8881 atttttcttt caatggcttc tcttttagct tttttaaata cttaatgaca tgttttgaag
     8941 gaacgatttc ataagccaat ttctgttgcc aactcctctt tagtcattac tttagaatta
     9001 gtaagtgtat cttcagcaat atctctaaag gaagaatgca ttttgttttt tctattctta
     9061 aattctacaa gaatctcatc tttatcgtag ccttctgcaa ttatatcaga caaaatatct
     9121 tcgctaaaat cataaaattc tttttgtggt tcaacaaatt cataaagaat accattctct
     9181 accaactttg cttcaacttc gactccatta ggaacattaa aatctttagg tactgtcaac
     9241 atgattgaat tgccttgttt tcttgttttt gtaatcattt aaatccctca tttctaacag
     9301 tatgtaatta cttagtaaat acattatacc acgagataat gtatttacaa gtaattactg
     9361 tgactcgtaa tgcgtccttg ctgaataaat ttcaaccacg tgttcatctt catctacctt
     9421 ataaacaacc cggtgttgtc tattcaatct tctaaaatac ctaccttcat gtgtaggttt
     9481 taacttctca aatgattggg ttctgttgca aagttttaaa taaagaataa aatcccttac
     9541 ggtatctatg atttaagct
//
LOCUS       DAZUJO010000099         9443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000099 DAZUJO010000000
VERSION     DAZUJO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.103"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..114
                     /locus_tag="ACG8OL_002520"
     CDS             <1..114
                     /locus_tag="ACG8OL_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4818453.1"
                     /translation="FISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            164..787
                     /locus_tag="ACG8OL_002521"
     CDS             164..787
                     /locus_tag="ACG8OL_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818454.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1237..2286
                     /gene="trpX"
                     /locus_tag="ACG8OL_002522"
     CDS             1237..2286
                     /gene="trpX"
                     /locus_tag="ACG8OL_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS4818455.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2283..3164
                     /locus_tag="ACG8OL_002523"
     CDS             2283..3164
                     /locus_tag="ACG8OL_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818456.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3167..3937
                     /locus_tag="ACG8OL_002524"
     CDS             3167..3937
                     /locus_tag="ACG8OL_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818457.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4216..5070
                     /gene="aroE"
                     /locus_tag="ACG8OL_002525"
     CDS             4216..5070
                     /gene="aroE"
                     /locus_tag="ACG8OL_002525"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS4818458.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5248..6411
                     /locus_tag="ACG8OL_002526"
     CDS             5248..6411
                     /locus_tag="ACG8OL_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS4818459.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6528..6938
                     /locus_tag="ACG8OL_002527"
     CDS             6528..6938
                     /locus_tag="ACG8OL_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4818460.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            7112..9340
                     /gene="topB"
                     /locus_tag="ACG8OL_002528"
     CDS             7112..9340
                     /gene="topB"
                     /locus_tag="ACG8OL_002528"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS4818461.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         3291 a         1609 c         2029 g         2514 t
ORIGIN      
        1 ttcatctcat ccgttgcgtc caaaagaaac catatcccta gaaaatcact aaattaccaa
       61 acaccattag aagttttttt gagttacgta aatggaaagt tttgtctcgc ttaatttgac
      121 aaataatata taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga
      181 agtagttaaa tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa
      241 tccaagtaag tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt
      301 tgcaatgttt ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc
      361 gtccatacca accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa
      421 acaaagccgg gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat
      481 gcttgatgtt tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta
      541 tccgatcgtt tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt
      601 gacatttatt ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg
      661 aataactgat tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat
      721 caaacaatta gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc
      781 taattgagac attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag
      841 aacaagtgat gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa
      901 attcaaaaat tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact
      961 tatcaaaacg tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg
     1021 tattaatggt gagactttcc catagataca actttttaga gtgcatttct aaagcttgta
     1081 tctatgctag acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt
     1141 aaagacaatg accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat
     1201 aataaataaa aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca
     1261 gttgtagtaa ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc
     1321 gaaaaagaaa gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa
     1381 tacgtgagcc atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa
     1441 gaaggattaa aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag
     1501 agcaagctag ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga
     1561 atcgcaacac ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta
     1621 ggtgctgtaa cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt
     1681 aatgtaacag gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat
     1741 ttgttgccaa atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa
     1801 taccaagtag cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat
     1861 gcggtgcctt ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat
     1921 tttatctatg ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa
     1981 gcaaacaaag caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga
     2041 ctagcaacaa tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca
     2101 gaacttgcga gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg
     2161 gatacagtga tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg
     2221 aaggaaaaag ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac
     2281 aaatgatcgt atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt
     2341 tcatgactta tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag
     2401 gtgctgctgt ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat
     2461 taggcgtagg tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa
     2521 agataccggt tattttagca gggattttag tgatgtcagg attgaactca gttattttgt
     2581 ttgtgatgcg aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc
     2641 atcgctttgg gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca
     2701 tcttgattgc tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga
     2761 caggagacaa cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt
     2821 taggtttaac tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg
     2881 acggatatgc agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca
     2941 tcatcggtga agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg
     3001 tcggcagtat catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga
     3061 cttatctgaa gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga
     3121 agaaagcact gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt
     3181 gacaatcaaa aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt
     3241 ggatgaaatt aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg
     3301 tgctggaaaa agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc
     3361 gatctctttt aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct
     3421 ttctcgagtt ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa
     3481 tttgctactt gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa
     3541 tcaaaaagaa gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt
     3601 ggatactcca gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc
     3661 gacgatcaca gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa
     3721 aacagctaaa gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg
     3781 tctcatgatc acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact
     3841 ccaaaaaggg aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg
     3901 cgaccttttg cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca
     3961 gtagaaggac ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat
     4021 aaacaattaa acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta
     4081 aatagaatat tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac
     4141 ctagacgttt cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa
     4201 caaaggagga ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg
     4261 gcttctcaca gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat
     4321 gcggtgtatt tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt
     4381 cgaacttttg atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct
     4441 tatctggatg aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac
     4501 gaaaagggtc gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa
     4561 gaagctggga tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca
     4621 aaggcagtca ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga
     4681 aacaaccaaa cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct
     4741 tgtaagataa aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa
     4801 agctgtcttc tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg
     4861 ctcttagata taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct
     4921 agagaaacac gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt
     4981 ggcatgttga tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg
     5041 atccatgtga ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc
     5101 tcgcataaac ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt
     5161 ttttattaaa ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt
     5221 atcagtaaga aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat
     5281 aagaaggcga aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa
     5341 cgaacgcgta tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca
     5401 tttgaacatt tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa
     5461 caacaaaaaa ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga
     5521 agaaagcgat cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca
     5581 ggtatctcat tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg
     5641 acttcagaaa cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt
     5701 ggaagtgata cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg
     5761 gatggtcctt cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc
     5821 ccaggggtag gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg
     5881 cgtcaaactt tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc
     5941 aaatcgtttg aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa
     6001 aaagatctga atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat
     6061 gttcttttgc agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga
     6121 cgccaacagc aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc
     6181 cgtgcacctt atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg
     6241 ttcttgacga aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt
     6301 gtgccagtag acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa
     6361 attgataatc aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa
     6421 agatcaagaa gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa
     6481 aaaacgcttt cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc
     6541 tgaacttcaa aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg
     6601 aagcatcagg tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag
     6661 aagaagcaaa aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt
     6721 ttgcagatac gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag
     6781 tcatccagac aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg
     6841 gtaagattga gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg
     6901 atcgatttgg catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct
     6961 ggaaacatca agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc
     7021 gactatacgg tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt
     7081 ccgcaaaaaa ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc
     7141 ttcacaagca aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta
     7201 tgaaatcagc gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt
     7261 ggtagatatg gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa
     7321 tttgcctatt ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt
     7381 ttcgatcgta aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga
     7441 tcgagaaggt gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga
     7501 aaagaactat ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt
     7561 taagaatctc caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca
     7621 gaacgctgat tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg
     7681 aaaagggatt gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat
     7741 ttatcagctg caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg
     7801 tttaattacg acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa
     7861 aacccaagaa gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa
     7921 cggacggatt cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc
     7981 gctatctagt ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact
     8041 tgatgctgct caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt
     8101 ttacattaca gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt
     8161 tttaggaata gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc
     8221 gaaaaaagtt caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg
     8281 ctttgcgaag ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt
     8341 ggctatgttt gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca
     8401 attaaagcta aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg
     8461 catcaagtca aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc
     8521 agaagtagaa ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg
     8581 aacgttgatc acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga
     8641 aattctcaaa gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa
     8701 tcttaagcag aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg
     8761 aatcactctt tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc
     8821 gcgctgggaa gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt
     8881 agagaatatc aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa
     8941 ggtagacttt tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa
     9001 cgatcaatta ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat
     9061 cgctacatgc ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa
     9121 gctgactaaa acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa
     9181 aggttttact ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac
     9241 aatcggtttt tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa
     9301 agaaacatca agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg
     9361 gaatatttat tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa
     9421 tcaagcaaaa acgttggggt agg
//
LOCUS       DAZUJO010000100         9403 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000100 DAZUJO010000000
VERSION     DAZUJO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.104"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..802)
                     /locus_tag="ACG8OL_002529"
     CDS             complement(134..802)
                     /locus_tag="ACG8OL_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS4818462.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            936..1223
                     /locus_tag="ACG8OL_002530"
     CDS             936..1223
                     /locus_tag="ACG8OL_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818463.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1639..3177
                     /locus_tag="ACG8OL_002531"
     CDS             1639..3177
                     /locus_tag="ACG8OL_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS4818464.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3174..4139
                     /locus_tag="ACG8OL_002532"
     CDS             3174..4139
                     /locus_tag="ACG8OL_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818465.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4126..5448
                     /locus_tag="ACG8OL_002533"
     CDS             4126..5448
                     /locus_tag="ACG8OL_002533"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4818466.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5511..5615
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5689..6432
                     /locus_tag="ACG8OL_002534"
     CDS             5689..6432
                     /locus_tag="ACG8OL_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818467.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6447..7439
                     /locus_tag="ACG8OL_002535"
     CDS             6447..7439
                     /locus_tag="ACG8OL_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS4818468.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7451..8188
                     /locus_tag="ACG8OL_002536"
     CDS             7451..8188
                     /locus_tag="ACG8OL_002536"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818469.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8178..8900
                     /locus_tag="ACG8OL_002537"
     CDS             8178..8900
                     /locus_tag="ACG8OL_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS4818470.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8984..>9403)
                     /locus_tag="ACG8OL_002538"
     CDS             complement(8984..>9403)
                     /locus_tag="ACG8OL_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS4818471.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3087 a         1613 c         1976 g         2727 t
ORIGIN      
        1 aaaaacaaac tattttcgca gatcgtttgc aaaaatagtt tattttttta taaagaagat
       61 tttactgaat aatatcattt tagtttacat aatgatattt ctgtaaatta aataaagtag
      121 tgaaaaataa gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg
      181 tagtctgttt tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat
      241 tcaatgtata agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca
      301 aataaagcaa ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct
      361 taatatgaaa atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc
      421 cgaatcgaaa gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga
      481 tcagtagact atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa
      541 ctagcgcaaa tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg
      601 agataagcaa taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa
      661 gattatgcaa aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat
      721 tgattcctgt cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattctg
      781 taaataaatt taattttttc atttctagcc tcttttcttt tgaaaatttt cagcaacttc
      841 tttattatac tgacttatac tcttacttca agaggaaaat gaaaaaaaat tatttttggc
      901 tgtttctttt tatttatcaa ataaggagtg gaaaaatgcg agcagcagac ttattgacaa
      961 ttcttacaaa atcacccaaa aaaacagagt atcaaggagt ttttttggct caagaacaaa
     1021 aactggtgcc tgtgacaatg gtccagataa ataaagcaaa tcaatttatc tttttatttg
     1081 aacacggcaa agcaccactt gctatgaaag acatctttct ttttttgatg aagaaccgtg
     1141 agaaagctgt ttattatcag tcaaaagaag aactcattcc tctttatgga gtgaaagaga
     1201 tagaaaataa agtggtgata taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa
     1261 caaccatttt ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac
     1321 aggccaataa aggtgttctc ccccacgaga tttctacttt attggctgtt tctttttatt
     1381 aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag
     1441 atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct
     1501 tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt
     1561 aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat
     1621 aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag
     1681 ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca
     1741 ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta
     1801 ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc
     1861 ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt
     1921 ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca
     1981 gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca
     2041 ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact
     2101 ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga
     2161 aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc
     2221 agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca
     2281 gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa
     2341 accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa
     2401 gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg
     2461 agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt
     2521 actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa
     2581 gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta
     2641 aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa
     2701 catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc
     2761 acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg
     2821 aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga
     2881 gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg
     2941 actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa
     3001 gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata
     3061 acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca
     3121 gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta
     3181 aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc
     3241 taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa
     3301 aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact
     3361 tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg
     3421 tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc
     3481 ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa
     3541 aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag
     3601 aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg
     3661 acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt
     3721 ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa
     3781 tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac
     3841 cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga
     3901 aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa
     3961 taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc
     4021 ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga
     4081 caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat
     4141 gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca
     4201 ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga
     4261 tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg
     4321 agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa
     4381 cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc
     4441 gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa
     4501 gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg
     4561 gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa
     4621 gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct
     4681 gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg
     4741 tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt
     4801 gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg
     4861 gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta
     4921 gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg
     4981 caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa
     5041 ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca
     5101 agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct
     5161 gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt
     5221 accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt
     5281 gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc
     5341 gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc
     5401 atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa
     5461 ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc
     5521 tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag
     5581 ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc
     5641 atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat
     5701 ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt
     5761 tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca
     5821 gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg
     5881 gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta
     5941 gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg
     6001 ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg
     6061 ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag
     6121 caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa
     6181 ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc
     6241 agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata
     6301 cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta
     6361 ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt
     6421 tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt
     6481 tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt
     6541 tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc
     6601 tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag
     6661 gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt
     6721 atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg
     6781 ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt aacatccatt
     6841 caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg
     6901 tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa
     6961 gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag
     7021 gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag
     7081 aattagatga acggttagat tactatactc cggtaattat tacgaatgat caactgatca
     7141 aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag
     7201 ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc
     7261 ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga
     7321 attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg
     7381 gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg
     7441 agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat
     7501 attagaagac atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag
     7561 cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg
     7621 aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc
     7681 gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa
     7741 aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt
     7801 aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc
     7861 cttgttacga acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc
     7921 gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc
     7981 aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag
     8041 gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa
     8101 acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt
     8161 attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt
     8221 tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag
     8281 aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc
     8341 atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa
     8401 ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa
     8461 aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc
     8521 acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt
     8581 tgatccattg gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt
     8641 tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc
     8701 caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg
     8761 ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg
     8821 agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt
     8881 tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg
     8941 ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg
     9001 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
     9061 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
     9121 aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
     9181 tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag
     9241 caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa
     9301 caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt
     9361 gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAZUJO010000101         8957 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000101 DAZUJO010000000
VERSION     DAZUJO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.106"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            453..1025
                     /locus_tag="ACG8OL_002539"
     CDS             453..1025
                     /locus_tag="ACG8OL_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS4818472.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            1170..1874
                     /locus_tag="ACG8OL_002540"
     CDS             1170..1874
                     /locus_tag="ACG8OL_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS4818473.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            2102..2440
                     /locus_tag="ACG8OL_002541"
     CDS             2102..2440
                     /locus_tag="ACG8OL_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818474.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2425..3279
                     /gene="lgt"
                     /locus_tag="ACG8OL_002542"
     CDS             2425..3279
                     /gene="lgt"
                     /locus_tag="ACG8OL_002542"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS4818475.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3426..3659
                     /locus_tag="ACG8OL_002543"
     CDS             3426..3659
                     /locus_tag="ACG8OL_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS4818476.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3857..5761
                     /locus_tag="ACG8OL_002544"
     CDS             3857..5761
                     /locus_tag="ACG8OL_002544"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4818477.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPTEKVKIS"
     gene            6115..6480
                     /locus_tag="ACG8OL_002545"
     CDS             6115..6480
                     /locus_tag="ACG8OL_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFS4818478.1"
                     /translation="MTAEVCTTTCIHKEKVASVKNNLKDKDLSTLLVLGKCFSDSSRI
                     KIFYALETYKEMCVCDLAAILNASIATTSHHLRFLKKNGMAKSRQYGKVVYYSLANEE
                     ILSAVQAFLKLSENSSMKA"
     gene            6497..7108
                     /locus_tag="ACG8OL_002546"
     CDS             6497..7108
                     /locus_tag="ACG8OL_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFS4818479.1"
                     /translation="MLQNILSALAVYISTSIDYLFILLIIFSQNHTKKGLRQIFFGQY
                     LGTGILVAISLFAAYVLNFIPQDWIIGLLGLIPIYLGIRVAFVGEEEEEEGEVVEKLG
                     SRGTNRLFWTVSLITIASGGDNLGIYIPFFTSLSFSEIVTSLIVFAISVAVLCYISYK
                     FAKISFVSETLEKYERIIVPIVFIGLGIFIMIENGTIQTILGL"
     gene            7136..8797
                     /locus_tag="ACG8OL_002547"
     CDS             7136..8797
                     /locus_tag="ACG8OL_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4818480.1"
                     /translation="MSENKKYHEWILEGIDCANCADKVERGVAKVAGVANSSVNYMTE
                     TLSFEVTEEKEYLVLSNVQQKVKKLEPDITLKNKVDGRLIEINPALIKKSNLKDDKKN
                     YEWILEGIDCANCAAKVERGVATVPGVTNSSVNFMTETLSFEVAGENKTDVLASVKQT
                     VGTLEPDVVLRKKSDGTLIEHGTDSNKNSTSNKSISDKDETNQKQTNRAYLASLLGNK
                     TKITLIRLILGLGILATGIFLPMDGQASLGLFILAYLITGYDVVGRAIRNIFRGQVFD
                     ENFLMTIATLSAFYIQEYPEAVAVMLFYQVGEVFQDIAISKSRRSIADLMDIRPDYAN
                     LVTGNNVEKVAPEAIKIGNIILIRPGEKVPLDGKVVEGASAMDTSALTGESVPRSVKP
                     EDTILSGFINKNGLLKVVVEKPFSESTVKKILDLVQNASGRKAPTEQFITKFSRYYTP
                     IVVIAAALLAIVPPLLVPGATFNEWIYRASIFLVISCPCALVVSIPVGFFGGIGSASR
                     KGILVKESNFLEGLNDLKYVVMDKTGTLTKGFCCKVLNLLSNKVE"
     gene            8825..>8957
                     /locus_tag="ACG8OL_002548"
     CDS             8825..>8957
                     /locus_tag="ACG8OL_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4818481.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     "
BASE COUNT         2891 a         1448 c         1957 g         2661 t
ORIGIN      
        1 gtatacccta aatagacatc ataataacat tgaaaaacat ggttgtcatt tttctagata
       61 aatggcataa attttcagcg ctaagtgaca aaagcaacta atttttcatg agagattttt
      121 aaatatctca atctatctcc aaaattagtt gtttttgtag ctatagttga ctatgaatat
      181 gacaaataat tagaaaaaac tgtgccaatt aagtataaaa atgacatttt aggccctact
      241 ttaatgaaat ttttgtttgg agggaacgac ttcattcctt tgattatttc agttgaattt
      301 aatagagaag cctggcgtct attaagaaat aattttcttg atgagcgtca atttttttaa
      361 cggaaatcta tcctatacta aattcagtaa agaaaaagga ggaatcaaag aaagctattt
      421 taaacaaacc aaaattatag gaggaataaa tcatgactga aaataaagct actcaagaaa
      481 gaacacccca agaaagattg caagaagaac aagaacacaa ggaacatgtt catcatacga
      541 aaattaatgc agcggccatt gcggatcatc ttttaggcaa catgcataca ttacatgtga
      601 aactgcacca atatcactgg tatgtaaaag gagccaattt ctttacgttg catgaaaaat
      661 ttgaagagtt gtataacgaa aatgaaaaat ggttcgacaa acttgcggag cgtctaatta
      721 cttctgggca taaacctgct tcgacaacgg ttgaatttga aaaatattcg atgctttcgg
      781 aagatgcagt taataaatac gctaaagcag aagacatggt tgaaaatatt atcgaggact
      841 tcagaagcac tcgtgaccta actattcgcg cgattcgttt agctcaagac gagggtgacg
      901 atgctttaga agatacattg attgctttta aaaatgactt agacaagaac atttggatgc
      961 tgcaagcgtt cattggtaaa gaagcattag aagatgatga cgctcttgat gaggatgaag
     1021 attaaggtaa aagtagaagc tgatttaggg attggaattc tttccagtcc tttttccatt
     1081 ttcttgatgt gcatcaattt ttttgtagta agagacttat ataataaaag cagcgaatat
     1141 aaacaaaata aataaagggg gagaatgaat tgactaaaca tacccatcat gaaattggcg
     1201 accacgcagc ctgtatccgc ttggttccaa tattcaatca cttagaggat agcgcgatga
     1261 attacattgc tgaaaaagcc cacacgaaac agtatcaaaa aggagagttt ctgtttcgtt
     1321 cacaagaaaa agaggatact ttatatatta tcaatcgtgg gaaaatccgt atttatcgat
     1381 tggcagactc tggcaaagag cagctagtgc ggatcttgaa ccctggtgat ttcaccggag
     1441 aatggacctt gttcaacccg gatgcagtac acgaggaata tgccgaagca acccgagata
     1501 ccgttgtctg tatgattcag caaaaagatg tccaagattt tctggaacaa tacccagcta
     1561 tttctttaaa actattggcg gaaatgtcta aacggttaga aagttcagaa cgtcaaacga
     1621 cacaagtagc cgtggagagt gtcattaccc gtttagtttt gtttttggca gaaaatgtgg
     1681 aacctgaaat gggcaactct cccaccatca ccctgccgat ggcaaaaaag gacattgctt
     1741 cctatttagg aacgacacct gagaccatca gtcgcaaatt tggagaattg gaggacgaag
     1801 gattgattca acagctgtct gggaaaagga tcaagattca ggatttagat gatttattgc
     1861 tttacagcga ataatcggac acactttttt ctacacgttg gtgttctgtc gctcttcatt
     1921 aacaacggga cagaactcag tgcttttgct tccatctgtc cgtttttggc ttttttaaga
     1981 cagaacgacc ttcattcatt ggaaaaataa aagcacaaac atgataaccc tcaagttttt
     2041 ttgttttatt tttctttaag atagggttgt cgaacaaaac gtaaaaataa gtagggtgaa
     2101 tgtgcattgt ttttgggtga tagttgccat cacgttgtca gcagcggggg cattctttgt
     2161 cgtccctttc accaagaaca cgattggcgg cgaagcaaaa acgtctaacg ttgttgaggc
     2221 agataggtta gctatggaaa aagaagagga agttgtaaaa ggagccggcg taatcggaca
     2281 cggaaaagtg gcgtattacg aagcgagtga ccagggagat aaaagtgtat tcactgtttg
     2341 gtttccatgg agcttagccg gaatcttgtt cagcatcacc atgactagaa tgacgcgaaa
     2401 aattgaagaa atggagcaaa tagaatggga ttcgtattag gcgttttgaa tccccatgtg
     2461 gtggagattg gttctgttgt gattcattgg tatggagtca ttattgcagc gggcatatta
     2521 gcggctctcc agttgagcac caaggaagca aagaaaaaag gactggaaga ggataccatt
     2581 atcgatatgg ccttatgggc aattccgatt ggactcctcg gcgctcggct ttattatgtg
     2641 ttatttgaac ttggttatta tctgcaaaat ccaggacaaa tccttgcgat ttggaatggc
     2701 ggcattgcga tttatggggg attgattgca ggagggggta cgctatattg gtatgcaaag
     2761 aaaaaaggta cttctttggc actcgttctt gatattttag cgcccaatgt attgttagcc
     2821 cagtccattg gtcgttgggg caattttatg aatcaggaag ctcatggtgg gccggttact
     2881 cgtcaattct tggaaaatct ttatttacct gaatttatca ttgaacaaat gaatatgaat
     2941 ggtacctact accatccgac ttttttatac gagtcattgt ggagtttgtt gggctttgtc
     3001 ctcatcgtca ctatacggaa ccgaaaacag cttttgcgtc aaggagaagt cgctttgagt
     3061 tacgttttgt ggtactcagt gggtcggttc ttcattgaag gtatgcgaac ggacagttta
     3121 tggattggcg agtggatccg cgtttcgcaa gccttgtctc tggccctgtt tattggcgcg
     3181 attgtagtat ggtttatacg cagacaagat tatccaccaa tttcttatta ttctgatggc
     3241 aataaggggc agaaaaagac tattaagatg gtgaattgag caaaaagata aaaagaagtg
     3301 caaaacttga tatgcgtcaa ttttcgtttg ctaaaagtga gctatactac agttgtaaag
     3361 aggaaaaggc agtgttcatt aatctgctgc cgataaaata aaaacacata aaggagatag
     3421 gaattatgga aaaagctata ttgcaattag aaacgttgtc ttgtccaagt tgtatgcaaa
     3481 aaattgaagg tgccgtgaaa tcagttaacg gtattgataa agacagcatt aaagtattat
     3541 tcaactccag caaagtcaaa accaattttg attcagctgt cacttcgatt gaagagattc
     3601 aaaaagctat cgaaaacgta ggctatccgg ttcttaaagc aaaagtcaag gccgtttaac
     3661 agattaaatg aactaggcaa ttctggaaga aaagctattt ttccaggatt gctttttttg
     3721 ttgcaatccg attcgaggtg cttagaaacg ataaaagaga actttctaaa cttgatgtca
     3781 gtcaattaat tacttaggca attatcttat actgtgttta tcaataaaag agaaattaaa
     3841 tttggaggga aaaagcatgc aacaatatat attaagcaag aaaaatgtta tcaccgtcat
     3901 cagtggattg ttaatcgtac tcggtttctt cagtcacttt gttttagaaa acgtaggact
     3961 ttcagagtgg tctttgatca tcgcctctgt ctttgggatt acgccaattg ccattcaagc
     4021 gtttcaagca atgaaagtca aagtcatcag tattgatgtc ttagtcagta ttgcagcgat
     4081 tggtgcgctt tttattcaaa attatgaaga atcggctatt gtcacgttct tattcttatt
     4141 cggacactac ttggaacaac gaacattgaa ccaaacgcga tctgctatca aagaattgac
     4201 tgaaatggca cccgaaagcg ccttgaaaca aatggataat ggcgaatttg aagaagtaga
     4261 agtggatgat atagatgaag gggatatctt gctcgttaaa acgggtgcga aagttccagt
     4321 agatggcata gtccttacgg gtgaagggca tatcaatgaa gctagcatta caggtgaagc
     4381 tattccggtc aacaagctag ctgatgcaga agtttttgca ggaaccattt tagaaaacgg
     4441 aacgattcaa atcagagctg accgagttgg tgaggacacc acatttggaa aaattattga
     4501 actcgtggaa gaagcacaag attctaaatc cgaagcggaa cggttcattg atcgcttttc
     4561 taaatactac acaccagctg tcttggttct ggcaattgtt gtatgggcgt tcagtcaaaa
     4621 tattgagtta gcaatcacca tcttggttct aggttgccca ggcgcattag ttatcggagt
     4681 gcctgtctca aacgttgccg gtattgggaa tggtgctcgt aacggtgttc ttttaaaagg
     4741 gagtgaagtc attcaagact tcagtaatgt ggatacaatt gtatttgata agacaggtac
     4801 tttaactgtc ggaaacccaa ccgttgcagc gactgaactg tatgaaaaag atcctgctga
     4861 aacgcttggc tatctggcca gtgtggaacg agaatcagat catccattag caaaagcggt
     4921 cttaaatcaa attggagaaa caaactttta tcctgttgaa ggaactgaag ttgtgaaagg
     4981 cggcggaatc gtttcaagtg tagctggaca tagagtggct gttgggaatg tggccttgat
     5041 ggaaaaagaa aatgtcactc tcagcaaaaa agtgcaaaaa gatgtcaaac ggtttgaaca
     5101 acaagggaac tctctcgttt tgacagcggt cgacggtgaa ttaaaagtac tgatgggcat
     5161 tcgcgatcaa gtccgtccgg gtgtgaaagc taatttgcag gaattaaaag acttgggcgt
     5221 gaaaaatcta gtcgttcttt caggagataa ccaaggaacc gttgatgtgg tcgccggcga
     5281 acttggtttg accgaagctc acggctacat gttgccggaa gacaaatcgg cttatatcgg
     5341 aaaacttcaa caacgtggtc aaattgtagc ctttgttgga gatggcgtta acgatagtcc
     5401 gtccttagct ttagcagaca ttggaatcgc aatgggaagc ggaacggacg tagcgattga
     5461 aacatccgat gtcgttttaa tgaactcgaa cttcagcaac ttgcctcacg cattaggatt
     5521 agtaaaagcc accgcaaata atatgaaaca aaatatcgtg atttcagttg gagtagtgtt
     5581 ggtcttgttg accagcgtct tcttcagcga atggatgaat atgtctatcg gaatgttggt
     5641 tcatgaaggt agtatcttgg tggtaatttt caatggcatg agattaatga agtataagtt
     5701 gaaaggtcgg aaagaacaaa aagaagtatc agcacctaca gagaaagtaa aaatatctta
     5761 agaataaatt agaagaggct gagacaatag tccatccttg aaatgacaaa agcggaaact
     5821 cagattttta aatctgggat tttccgcttt tttgagttct tgaaaaaaaa taagagtata
     5881 gagtatgttc tttataaaat taattttcgt gtaaattatt aaaaatttaa taataaaatt
     5941 taaattccta ttaagttgta acacatgaaa ttgcttaaaa tagtgtatca aaaaccttgt
     6001 tttattaatg ttgaatttat ttgaaaggtc tacagattaa catattattg acaaaacaat
     6061 atttaaatag ttatacttca ttcaaacgtc tgtttgaacg aggtggtgaa gttaatgact
     6121 gcagaagtat gtacaactac ttgtatacat aaagaaaaag tagctagtgt taaaaataat
     6181 ttaaaagata aagacctttc aactttactt gtattaggaa aatgttttag cgattcttct
     6241 aggattaaaa ttttctatgc tttagaaacg tataaggaga tgtgcgtttg tgatttagca
     6301 gcgatactta atgctagtat tgctacaacg tcacaccatt tacgtttttt gaaaaagaac
     6361 ggaatggcga aatcacgtca atatggaaag gtagtttatt actctttagc gaatgaagag
     6421 attctttccg ctgtgcaggc ttttctaaag ttgtcagaaa atagttctat gaaagcttag
     6481 tttggaggta gttattttgt tacaaaacat tttatcagca ctcgctgtat atatttctac
     6541 cagtattgat tatttattta tcttgctgat tattttttct caaaaccata ctaaaaaagg
     6601 tctccgtcag attttttttg gtcaatatct tggaacagga atcttggtag ccataagttt
     6661 gtttgcagca tatgtgctaa attttattcc tcaagattgg atcattggtc ttctgggact
     6721 aattccgatt tatttaggga ttcgtgttgc ttttgtgggc gaagaggagg aagaagaagg
     6781 ggaagtcgtt gagaagctag gatctagagg aacgaatcgt ttgttttgga cagtttcctt
     6841 aataacgatt gcttctggtg gcgataattt agggatttat attcctttct ttacctcgtt
     6901 atctttttca gaaattgtca cttctttaat cgtctttgct atctctgttg cggttctttg
     6961 ctatattagt tataaatttg cgaaaatttc ttttgtctct gaaacactag aaaaatatga
     7021 acgaatcatt gtacctatcg tatttattgg attagggatt tttattatga tagaaaatgg
     7081 aaccatccaa actatattag gtttgtagag atcttattag taaaggaagc aaacgatgag
     7141 tgaaaataaa aagtaccatg aatggatatt agaaggaatc gactgtgcaa attgtgcaga
     7201 caaagtagaa agaggcgtcg ccaaagttgc aggtgttgcc aatagtagtg tgaactatat
     7261 gacagaaacc ttgagtttcg aagtgacaga ggaaaaagaa tatcttgttt tatccaatgt
     7321 gcaacaaaaa gtaaaaaaat tggagcctga tatcacttta aaaaataaag tggacggtag
     7381 gctgattgaa ataaatccgg cattaatcaa gaaaagtaat ttaaaagacg ataaaaaaaa
     7441 ctacgaatgg atattagaag gaatcgattg tgcaaactgt gctgccaaag tggaaagagg
     7501 agttgctaca gttcccggcg ttacaaacag cagcgtcaat tttatgacag agacactgag
     7561 cttcgaggta gcaggtgaaa ataaaactga cgtgttggct agtgtaaaac aaacagtagg
     7621 aactttagaa cctgacgttg ttctgcgaaa aaaatcggac ggtacattga ttgaacatgg
     7681 aactgactct aataaaaatt cgacttctaa caaatcgatt tctgataaag atgagacaaa
     7741 ccaaaagcag acgaatagag cttatctggc cagccttctc ggaaataaaa ctaaaattac
     7801 ccttattcgt ttaatactag gattaggaat attagcgaca ggaatatttc tccctatgga
     7861 tggacaagcc tctcttgggt tgttcattct ggcttattta atcaccggtt atgacgttgt
     7921 aggacgtgcc attagaaata ttttccgagg tcaagtattc gacgagaact ttctaatgac
     7981 cattgcgacg ttaagtgcct tctatattca agagtatccc gaagctgtag cagtcatgct
     8041 attttatcaa gtgggagaag tattccaaga tattgctatt tcaaaatctc ggcgctccat
     8101 tgcggaccta atggatattc gacctgatta tgccaattta gtcacaggaa acaacgtaga
     8161 aaaagttgct cctgaagcaa ttaagattgg aaatattatt ctcattcgtc cgggcgaaaa
     8221 agtgccattg gatggaaaag tagtggaagg tgcttctgcg atggacactt ctgctttaac
     8281 aggcgaatcc gttccaagaa gcgtgaagcc ggaagacact attttgagcg gatttattaa
     8341 taaaaacgga ttgctaaaag tagttgtaga aaagcccttt tcggagtcta ctgtgaagaa
     8401 aattttagat ttggtccaaa atgctagtgg ccggaaagca cctactgaac aattcattac
     8461 caaattttct cgctactata ctccaattgt ggttatagct gctgccttac tggcaattgt
     8521 tccgccattg ctagtaccag gcgctacttt taatgaatgg atatataggg ccagtatctt
     8581 tttagttatt tcatgccctt gtgctttagt cgtttcgatt ccagttggtt tctttggtgg
     8641 gattggttct gcttcccgca aggggatttt agtaaaagaa agcaatttcc tcgagggttt
     8701 gaacgatctt aaatacgtgg taatggacaa aacgggtaca ttgacaaaag ggttctgttg
     8761 caaagtttta aatctactat caaataaggt agaataatag aaaaagatag taggaggaat
     8821 gacgatgaat cattttaaag gaaagcaatt tcagcaggat gtgattattg tagccgtggg
     8881 atactatctt cgttataacc ttagctatcg tgaagttcaa gaaatcttat atgatcgtgg
     8941 cattaacgtt tctcata
//
LOCUS       DAZUJO010000102         8939 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000102 DAZUJO010000000
VERSION     DAZUJO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.107"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..895
                     /gene="purR"
                     /locus_tag="ACG8OL_002549"
     CDS             77..895
                     /gene="purR"
                     /locus_tag="ACG8OL_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS4818482.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1398..2771
                     /gene="glmU"
                     /locus_tag="ACG8OL_002550"
     CDS             1398..2771
                     /gene="glmU"
                     /locus_tag="ACG8OL_002550"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS4818483.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2865..3836
                     /locus_tag="ACG8OL_002551"
     CDS             2865..3836
                     /locus_tag="ACG8OL_002551"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS4818484.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3842..3944
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4010..4579
                     /gene="thiT"
                     /locus_tag="ACG8OL_002552"
     CDS             4010..4579
                     /gene="thiT"
                     /locus_tag="ACG8OL_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS4818485.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4660..5433)
                     /locus_tag="ACG8OL_002553"
     CDS             complement(4660..5433)
                     /locus_tag="ACG8OL_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818486.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5700..7380
                     /locus_tag="ACG8OL_002554"
                     /pseudo
     CDS             5700..7380
                     /locus_tag="ACG8OL_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            7350..7568
                     /locus_tag="ACG8OL_002555"
     CDS             7350..7568
                     /locus_tag="ACG8OL_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS4818487.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7588..8478
                     /locus_tag="ACG8OL_002556"
     CDS             7588..8478
                     /locus_tag="ACG8OL_002556"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS4818488.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8496..8780
                     /locus_tag="ACG8OL_002557"
     CDS             8496..8780
                     /locus_tag="ACG8OL_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818489.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3016 a         1532 c         1882 g         2509 t
ORIGIN      
        1 cataaaatgc tcgaattttt gaagaaaaac ttatataatg taaaacgata aaatttctta
       61 aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca tgacacagta
      121 tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac gttatgaatc
      181 tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta aagaacgagg
      241 gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca tccctgaaat
      301 ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat cagaacaaga
      361 ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac ctgaactgct
      421 gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg atgctgtaat
      481 gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc tgaatgcgcc
      541 attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa gtgtcaacta
      601 tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa gtttgaaacg
      661 tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag tcaacggaat
      721 gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat ttgccgaatc
      781 aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg tcaaagatgt
      841 agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag aataagaggt
      901 tgtgaaaaag ctgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca
      961 ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttatctagag
     1021 gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa ttgctcctca
     1081 tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg tttaatgaag
     1141 aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa aacagctttt
     1201 catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca ccggttacca
     1261 aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg ggataaaaga
     1321 aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc agcaaactat
     1381 atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca gggaaaggta
     1441 cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa cctatggtag
     1501 aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg atcgttggac
     1561 acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg ttacaagcag
     1621 aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa ggaaaaaaag
     1681 gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg ctgaacaatc
     1741 tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca caagcagaaa
     1801 acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa aaaatcgtgg
     1861 aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca ggaacttact
     1921 gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac aatgctcaag
     1981 gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat acagttgctg
     2041 cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc gcgttagcta
     2101 aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat ggcgtaagct
     2161 ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca gacacattga
     2221 tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt gtcatcgggt
     2281 cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa aactcagtca
     2341 ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat ttacgaccaa
     2401 aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa aatgctcaaa
     2461 tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg ctgggagaag
     2521 aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac aaacatcata
     2581 cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga cctgtcgagg
     2641 tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt ccagaatatg
     2701 cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa aaactccctt
     2761 atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga ctactattta
     2821 ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca aaacattact
     2881 ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca gagaaaattg
     2941 cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt gatggagaaa
     3001 ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt caatcaacga
     3061 gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca ctgaaacgag
     3121 caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt caagaccgta
     3181 aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt caaaaagctg
     3241 gtgcaacaag aatgctgact ttggaccttc atgcagttca aatccaaggt ttctttgata
     3301 ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta gaaaaaggaa
     3361 tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca cgggcacgta
     3421 aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt ccaaaagcaa
     3481 atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt gtactgatcg
     3541 atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg aaagaagctg
     3601 gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct gctttacaaa
     3661 gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat cttccagaag
     3721 atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga gatgcaatca
     3781 aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat aaataataaa
     3841 taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa acccttcgta
     3901 cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac tattttcgaa
     3961 agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta tgggaaaaaa
     4021 tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt cgttgattcc
     4081 gatccagatc ggcagcagtt tttctatttc attagggcag attccattga ctctttttgc
     4141 gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca tcctacattt
     4201 tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt acccgattgc
     4261 ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag gctatgtaga
     4321 cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg gcgcactttc
     4381 cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg cactatgggg
     4441 gatgaatcct tggctctttt ctttcgtaat gaatggtgcc agcgggttga gtacagctat
     4501 tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt ttgtgccaga
     4561 taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa tcatgtccta
     4621 gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg gcagataaga
     4681 aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga ttaaagtagc
     4741 gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat cttcaatcac
     4801 atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag aaaaaatgat
     4861 cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt tttcaacagg
     4921 tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga tcgaacttaa
     4981 tttactagca aaaatcctat aaaattcatc taaatagcga taataaatcc gattcagctg
     5041 atcattaaga tcgaagtata caaaattatt ctgtagttta taaaaaaacg gcgaatgaag
     5101 atgtgttttc atatgaccaa aatataataa ttctgaaatt tctaaaggtg tcaattcttt
     5161 cagcatcgtt aaatcagtaa agtcgatcca cttcaattca tcgaatccac gcctgttacg
     5221 agaagaggaa aagaaacgct gaatattttc tgtaccacgg attactttca gccctgtatg
     5281 ggattcatat tctccagcag aagcagatgg atcaagcaag agcaagtttt gaggaaaatg
     5341 cacgatactg tttgcaaagt cagtagcagt cagccctttt gttaaaacag cattacttgt
     5401 ggtatcaata tgtacataaa ctgactcagt catcatgttc ctcctcttat ttcctcttaa
     5461 tctcttttat tttacaacat ttgcattaaa aaagcttgtt tcttttattt taaaattcca
     5521 ttacaaaatc acgttttcat tgaaaacact ctcattttca attaattttt gtgatttttg
     5581 tattttttta tttaaaggag gttttatgaa ttgttttctt atggaatcta caaagtcgaa
     5641 tacgagactc atgacctttt tatgacgtac tggtatactt gaagaaaaag gagagcgcga
     5701 tggagataaa aaaagtttac ttttcacaga aaatgtcgct gtattcgcct atccagcaag
     5761 caaaacaacc ccttccattt tttcaaagtc atcaagaaag tagagacgct ttttggcgtt
     5821 ctgcaaaaaa gcaatgtgct tttaaagcag tatcgaaaaa atataccgtc tattttacta
     5881 ttgctaaaag tagaggagat gttattttta ataatctgtt gattgaaggt gaaacttttg
     5941 aatggaaaaa attttttcgg tatatggaag cttgcgctcg tttttttatt aaagaaaact
     6001 gttgtttttt atttcaatct cctctatcag aagaatggtt gcgtattttt cataaaaacg
     6061 gctatcaagg aaccacacaa ttaaataaaa agctggtcta tcatacagca cttgttttag
     6121 gtggaggagg agcgcacggt gcttatcaga ttggtgtgtg gcaggcgctg aaggaacatg
     6181 atatcaactt tgagattatc acaggtacct cggtaggtgc gttgaacggc gcattgattt
     6241 tacaaggaga tatgaaaaaa gctgtcgaca agacaagtgc ttgcgttacc agaaatggct
     6301 gcagttgaag accttcgtga acgcttctat cgagaaagac gccaaatgac gaaaacagca
     6361 ctaattgaag gcggggtttc ttctgctcca ttggaaaaga tggtcaaaga caatttggat
     6421 ttgtccttac tcaaacataa tcccaaaata cgtttatata cggtgtctac taaactgcca
     6481 gaactagcgg aagtagtgac tgatatccaa cagacaaaaa cagaagaaat accagattgg
     6541 atactcgcct ctgcttcatt ttatcccgct atggcttatc gaaagatcgg aaaaaataaa
     6601 tatgcagacg gtgggtatcg caacaaaata cctattgata ttgcgattaa caaaggagca
     6661 acagaggcat ttgtcgtaga tgtgcaaggg ccaggcccag cgaaaaaaat tcgtatgcca
     6721 gacattttta tccattggaa atgtcaaaca ttgtggacac taggcagctt tctgcttttt
     6781 gatagccaaa ggaaccaatt aaatttacaa ttaggctatc tggaaatgaa aaaacgatta
     6841 ggctattact atgggaactg gtatacattt cattctgtta aacctgcaag aacatactgg
     6901 cgaggatttt tgtcttactt aacaaaagaa attcagttag aattatcttt tttcaaaagc
     6961 ataaaatttt ggcaaaaact gcgtaattta tataagaaca gggtcgtacc agagacatgc
     7021 ggattggcga tgctagaact tttagcaaaa aaacattttt tactgcccaa tgaaatctat
     7081 caagtagaca tgatgattca aatgatctgc caacaagata taaaaagtat gccggatgat
     7141 ttattggcac aaattggtca attaagtact gaagaatggc gaaggtatcg aaaataccag
     7201 caaaaaatac aaaatgagcg cacgaaaact gtttattttg attatctgct acaaggaaaa
     7261 cgcaaggatc gtttgcagca tgaattgctt aagcaaccag ttgacacatt actgatccta
     7321 tatttatatt atctcaagga ggaacaacca tggcacaaga attttcatat gaaatcgtag
     7381 aagaaatcgc tatattgtca gaaaacaaca aggggtggcg taaagaactg aacctagtca
     7441 gctggaatgg tcgcccgcca aaattcgatc ttcgagattg ggcgccagac catgaaaaaa
     7501 tggggaaagg cctgacattg acgaacgaag agtttgaaca actgcaaaaa gcaatagaaa
     7561 atatgtaatg aggaaggacc ctgtcgaatg aaaagtatga ctggttttgg caaagccatc
     7621 cgcgaaacaa aagcgtatca gttagatatt gaaataaaaa gtgtcaacca gcgttttcta
     7681 gatgttcaaa tacgaagtcc taaaatcctg aactttgttg aaaacgattt tcgtcagctt
     7741 attaaacagc agttaagccg cggtcgaatc gaagtatttg tgaatctagc ttatattggc
     7801 aaaaacgaaa aacagatcgt catcaactgg gaattgattg attcacttat cactcaattg
     7861 acagacggaa ttcaacaacg atacggtagt caaaatcaag tgtcaattag tcggatggcg
     7921 gaacgcttcg cgctggacga aaacttcgta acggtagaag aaaaaccgga agagagcatg
     7981 gaagaattgg cacagttagc tctagataca ttgaaaagcg cacttgattc tattgaggcg
     8041 agtcgagaaa aagaaggtca agctttagct ttagtgttga aaaagaatac tgaagaattc
     8101 aaagaagtac taaaaaaact cgatagtttt gtagaaatct atgaaaagga atttcaaata
     8161 aggtatcaaa aaaagctgga ggaatttctt ggagcgaaag tcgatgagca acggttgttg
     8221 acggagctag ccatcttgtt ggaacgcgga gatattcacg aggaactgga tcgtctggcg
     8281 atccatgtac aaaaactaga tgaacttatc gaagcacaat gtcctgtagg tcgggaatta
     8341 gatttcctga tccaagaaat gaatcgggaa atcaacacga tcggttccaa atcaaatgca
     8401 atcgaaatta aaaatcaagt cgtccagtct aagacgatct tagaaaaaat cagagaacaa
     8461 gtacaaaata ttgaatgacg aaagaggtag agataatgaa atattatgac gtaacgttcc
     8521 acgaattaag tggaaaaact gtggttaaac gggatatccc ttctgaaaaa aacggctttg
     8581 acgtatggaa agatgcctgt gcagactata atgagaacga actgtttatt ttgatcaacg
     8641 acggtgctta cgtgacgatg aatcggaaat ttattgttcg aattgacaca gaagaggtag
     8701 aagatccgac agaaaaagca cgttctcgaa aagatgagat catgggtgta gtgaatactt
     8761 tgtccaatat gggcttttag caaggatgct gaacaaagaa atacaataaa taatcagaat
     8821 tggatttctt cgcttagctt ctttgctcta ggaatacatt taaaggttgt gacaaaattc
     8881 gtgtcacaat ctttttttat tttgaataag tgatgttcaa aaaccatttt ctcggtatt
//
LOCUS       DAZUJO010000103         8774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000103 DAZUJO010000000
VERSION     DAZUJO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.108"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..2753)
                     /locus_tag="ACG8OL_002558"
     CDS             complement(372..2753)
                     /locus_tag="ACG8OL_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS4818490.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2743..3390)
                     /gene="recU"
                     /locus_tag="ACG8OL_002559"
     CDS             complement(2743..3390)
                     /gene="recU"
                     /locus_tag="ACG8OL_002559"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS4818491.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3454..3993
                     /locus_tag="ACG8OL_002560"
     CDS             3454..3993
                     /locus_tag="ACG8OL_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS4818492.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4083..4502
                     /gene="gpsB"
                     /locus_tag="ACG8OL_002561"
     CDS             4083..4502
                     /gene="gpsB"
                     /locus_tag="ACG8OL_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS4818493.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4524..4891
                     /gene="rnpB"
                     /locus_tag="ACG8OL_002562"
     ncRNA           4524..4891
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8OL_002562"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5100..6266
                     /locus_tag="ACG8OL_002563"
     CDS             5100..6266
                     /locus_tag="ACG8OL_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS4818494.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6312..7808
                     /locus_tag="ACG8OL_002564"
     CDS             6312..7808
                     /locus_tag="ACG8OL_002564"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS4818495.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7889..8572
                     /locus_tag="ACG8OL_002565"
     CDS             7889..8572
                     /locus_tag="ACG8OL_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4818496.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8581..>8774
                     /locus_tag="ACG8OL_002566"
     CDS             8581..>8774
                     /locus_tag="ACG8OL_002566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818497.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2772 a         1585 c         1850 g         2567 t
ORIGIN      
        1 acaaattatg ataattaagc atactatcag ataactcaaa atgaagcctt ctttcaaaaa
       61 attattcaaa accatatatt gtatttttta tttgataata acaatgggat tattaagaag
      121 tggcactaaa taaaagacta gtgtgtattt agccgattta attataaaca agtaaatgga
      181 taaagtatat gtaatgataa aagaaaaatg gattttctaa tttttaagct aaatgttaga
      241 tcacacatgt atatgctgga tagtcttata aaaaatcatg taatacagct tggtacatga
      301 tttttatttg tagctgtttt ttacatagac acaaaaaaga aaccacgctt gtagacgtgg
      361 tttctttttt actatctgtt tccagaagct gaggtgctgc tggatggtgt atggtcattt
      421 gcagcatccg ctccgctagt tgctggctca ctggaagaag caggaggagc agatgaagac
      481 ggtgtcgagc tttcttcgga tgaagaagaa ggaaccgttg ttgaagacgc tggctcgctt
      541 gtttctgctg tcgtgctttc ttttgaggac tcagcagttg attcgctttg actagtagag
      601 ctagagcttt gagtcgttgt ctctgttgtt gagcttccag gagtctggac atagctggaa
      661 gatggaatag ttgttgatgg tgtaattgca ttcgatctag ctgtatattg atctttgtag
      721 tacagttcac cgcccacacg aattaatccg cttggcatct cccagtctgt atttgtcacg
      781 ttagctgaaa catactgcat aagctcgcga taaacatcag aggctacgtg tgacgattct
      841 gaagtgacag gcgtcatttt tttattgtaa cctgtccaca cggaaatcga ataatttggc
      901 gtatagcccg caaataggat atctggatac accccagaag aaataccaag ttttgcatac
      961 tcgtcatctg tgtaattgga tgttccggtc ttaccggctt gatataaccc agcgatttgc
     1021 gcattcgtac cagttccttc agtgatcgta tcttttaaaa tgtctgtgat catataagct
     1081 gtttctggag acatggctgt ttttccatct ggctcataag tttcttctgt tccgtcttgg
     1141 aagacgattt tattaacata ttgaggtttg taataagtcc ctccattggc gaaagccgca
     1201 taggctgctg ccatttttaa ggaagaagct ccgtattttg tcccatcttg ttcttccgta
     1261 ttactagaga tcgcattcga ttgatggatc gtactgtatt cgatcccaag attttttaag
     1321 aaagaagcca ctttatctga accgacttca ttgaataatt tgactgctgg aacattacgg
     1381 gataagtaaa gtgcttggcg taaagtaatg gtgcccatat attgattatc ccagtttcct
     1441 acaggagtcg aagtcccttc atagttatac ggtgcgtcag taatagtttt tccagtagaa
     1501 tatttcaaat attcaaaagc tggtccgtaa tctgtgacag gtttcatcgt agaaccaaaa
     1561 tcacgggaag tattcactgc taagttgttg cctaaagtta cgtcttctgc gatgtgacgt
     1621 ccgccaatct gggcttttac tttcccggta ttggtgtcga taagggtgga ggcaacttgc
     1681 atctcgtcgt ctgggtaaga gacatactga tccgtgttga cgatatcata tagtttcttt
     1741 tgggcgtcca gatccaaatt cgtataaatc tctaaaccgt ctgtgtagac attcttatct
     1801 gttttttctt gtacttcatt gatgacttct ttgacataat tatcgacgat cttagtattg
     1861 tcatcggatt gagttagttc ttgcagacca tccgttaccg gtacattaac agcttgatca
     1921 tattctgttt gagagatttt ttcgttttga agcatcgtat acaaaacagt atctcgtctt
     1981 ttcttcgcct gatctggata aacataagga tcataggcgg aaggggcttg aggcatccca
     2041 gcaagcaatg ctgtttgcgg taaagaaagt tctgataatt tcttaccaaa gtacatctca
     2101 gaagctgtct ccatcccgta caagccgttt gacatataca ctttatttac gtagtaggtc
     2161 aagatctctt gtttcgattt tttttgttcc aatcttacag ccatccacgc ttcttgggcc
     2221 ttgcgtttca gagtctgatc ctcggcactg gtggagaaga aggaaagttt gatcaattgc
     2281 tgtgtcaaag tacttccgcc ttgcagtccg ccagaggtaa agttagagag ggcagaaccg
     2341 atgatccgaa tagggtcaac cccgatgtgt ttataaaaac ggcgatcttc taccgacacg
     2401 atcgcatctt ctaatgtttt cggtaattca ttcgcagaaa ttttttcacg cttttctgcg
     2461 cctaaatctt cgaatagttc gccatcttgt gtatatagtt tggaagatac ggtagcgtct
     2521 aattttttat ccgtcaattc gggcgcatct tttgcgtaat accagaaaag tcctacacct
     2581 gctaaaaaga ggatacagaa aaatgagagt attcctaaaa ggatcttgat aaataatcct
     2641 tttttatgtg ttccagagga ctttccagaa cctttgccag aattcttctt cacactcttt
     2701 ttaggagtag gctgtttctg tcgtctagag cttctagttt gttcatttgc cattcttgct
     2761 ttctccttca atagataatg ttgataaata gaagtcaacc gcgtccaaat aaggaatccg
     2821 cggagaaata ccagttttga tctctattcc tagttcttcg atcaaagata aaggtagtga
     2881 tttgcgtcct ttttcctttt gggcgtccca gtaagcaatc aaatgaacac tattcaaata
     2941 aaaacagcgc ttcaaagacg aaaaccataa caagacaaag cagaccccat tttgttcgag
     3001 gcatgctttc atatgttcga tttgatgggc atggaagttt ttaaagggaa aagatgtttt
     3061 gttcttggtt tcttttgctt caaagtccaa gtaaaagcct cgatagacgc cattatagtc
     3121 tgtagttgag gcttgggtaa agtaggcttc cttgatcaca gcagcacttc tcttaggata
     3181 gtctactttc acgatttgga taggggtagg ctttttatgg ataaccgcca acccgcggga
     3241 aagataatat ttattacttt cattgattgc ttcttcaaag cgcatccccc gattgccgaa
     3301 ttcagtgatc tgatttcttt tttcttgaat tttttcgacg ggagaggcat tcttataata
     3361 aggttggcca tttggatagc gcaataccat ctttaatcac actcctaggc cacattatag
     3421 caaaaaagcg gatagaaaga aaaggaatct gagatggata agtcaaaagt aatctatgtg
     3481 tctgggtatc gaagtttcga actcggtgtt tttaaagaaa atgatcctaa aatccaagtt
     3541 gttaaaaatg ttttaaaaaa agaactccat cagttagcag aagaagggct ggaatgggta
     3601 cttgtcagtg ggaatttggg cgtcgaacta tgggctgtgc aaatagtagc ggaattgaaa
     3661 gaagaatatc cggaactatc tcttggtatt ttgtatcctt tttctgattt tggtaataac
     3721 tggaatgaag ggaaccaaga aaaaaaacaa ttagcagaac aattagcaga ttatgtcgaa
     3781 gctgttagcc atcaacccta ccaatcacct agtcagctaa agaatcacac tcgttttata
     3841 cttgagcata cagtgggctg tctgctgatc tatgatgaag aattccctgg aaaaacgaag
     3901 tatttttttg aagatgcaca gaattaccaa gcagatcatc cctacgaaat tcggctgatc
     3961 agtatggacg atcttcaaaa ttttagtgaa taatcgatat tgtgtttgat ttatcaactt
     4021 ttttcctata caatgaaaaa ggtagaaatg ttattacgta aatgaaaact agaggtgtaa
     4081 atatggcgaa tttggtttat agtcctaaag acattttaca acaagaattc aaaacaaaaa
     4141 tgcgcggcta tgatcccgtt gaagttgatg aattcttaga caatatcatc aaagattatg
     4201 aaacttacag caaagaactc cttgctttac aagaagaaaa cgatcgattg agtgcaaaag
     4261 tagctcaatt atctaaaact caaggagcag ctcaaacacg cgtgcaacaa acagaggcac
     4321 caaagagcgc agcagtgacc aattttgata tcttaaaacg cttgtcaaat cttgaacgtg
     4381 aagtatttgg taaaaagctt gatcaacaag caagtgcagt caaaccagca caacctaatc
     4441 caaataacta cacaaatgca gacacaagct tagatgataa tgaaaaaaca cgtcaatttt
     4501 aatttttgat taatgaattt gcggttttcg ggtaatcgcg gtctgacttg atcaggctga
     4561 ggaaagtcca tgctcgcaca ggctgtgatg cctgtagtgt tcgtgcttag cgaaaccata
     4621 agctaaggta ctctttggag taacggcagg aaaacaagct aaggcttcgg ctatgcttaa
     4681 gtatccttga aagtgccaca gtgacgaagc ggctggggaa acccatccgg tggaacgcgg
     4741 taaacccctc gagcgagcaa cccaaacaat ggtaggggcg ctttttgtct ggaagaagaa
     4801 caggacaaaa aggcagtata actgcagata gatggttacc gctttgtttt ttctccctgg
     4861 aaaagcaaag tacagaacat ggcttataga aaaccgcaaa catcagggaa ttaggtcgtg
     4921 acaaaagtaa tgcgttataa gaaacaagaa aaacatttga aagctatttt tcaatacttc
     4981 agatgaatcg ttcttattac aaaacaaact tttgtatcac cggttattcg gaatttgagt
     5041 aaaagtgtgg ggtaggactg atgtcccacc tccttttttt ataaataagg agattgtgta
     5101 tggaaaaaaa caggaagttc aacctcgtcg caactgcggc aagcgggcta gaagcactgg
     5161 ttggtaaaga attaagagat cttggaatcg aatgccaagt tgagaacgga cgtgcacgtt
     5221 ttgaaggaac aatggaaacg atcgctacag ctaatttatg gttacgtaca gcagatcgag
     5281 taaaaatcgt ggttggggaa ttcgatgcgt atacgtttga tgaattgttt gaaaacgtaa
     5341 aagcattgcc ttgggaagat tttcttccac tagatgcagc ttttcctgta gctggaaaat
     5401 cgatcaagtc gaaactatat agcacacctg attgccaagc gatcacaaaa aaagcaatcg
     5461 ttgaacgtct ccgtacctat tatcaccgtc cagcttctgt accactgacg gaaactggcg
     5521 cacattttca gttggaagta gctttgctga aagaccatgt gatggtaact ctggatacga
     5581 caggaccaag tttattcaaa cgtggttatc gcttagaaaa aggtggagca ccattgaaag
     5641 aaaacatggc agcagcactt attatgttga caaattggcg aaaagaccgt ccattttatg
     5701 atcctgtatg tggttcaggt acgctttgta ttgaagcagc cttaataggc cataatatcg
     5761 caccaggatt caatcgtgag tttgcttgcg aatcttggga ttggttcagt caagaaatca
     5821 tggaaaatgt tcgatcagca gccgaagaaa aagcagatta tgatattgaa ttagatatta
     5881 ctggttcaga tatcaatgga cggatgattg aaattgccaa agccaatgca gaagaaatcg
     5941 gattaggctc ttcgattacc tttaagcaac aagcagtcaa agacttcaaa acggataaag
     6001 aatacggggt catcgtggct aatccgcctt acggagaacg tctaggagaa gaagaaactg
     6061 ttcggaaatt atataaagaa atgggcgatg tattccgtcc attaaaaaca tggagtaaat
     6121 atatcttgac tagcgactta gcgtttgagg aattttacgg acaaaaagca accaaaaaaa
     6181 gaaaattgta caatggtgca ttgcgtacgg atctattcca gtattgggga actcgtccgc
     6241 ctagaaaaaa agagaaagac gaataatctt actaattgat tcattcaatt ggatgtttgg
     6301 aggaataggg aatgaaagaa aaagagtttt tacaagaagt aaaagaaatc aatcttctac
     6361 aacaagcttt aggaatcctc gattgggata cacaaacagg tatgcctgaa aaagctagtg
     6421 cataccgaag tgaagtagac agctatcttt acagcattta tttttcaaaa aaaatcggtc
     6481 caaaaatcaa agaagccatt cgctattttt ctgaacatcc ggaagaattg tcggaagttg
     6541 gaactgccgt ttttgccaaa gtaaaagaag agtatgattt ggaacatcgc gtaccggaag
     6601 atcttatgag aaaatataca gctgccgttt cttcagcaca cggacaatgg caaaaagcta
     6661 gagaaacaca acaattttct gattttcaac acgcattgaa tcaaaatatt gaattaacaa
     6721 aacagttgat accttactgg agaaaagatg aaaagacgaa ttatgatgtt ttgctgaatc
     6781 aatacgaacc cgggatgact gtcgaaatcc ttgataaggt atttgatcag cttaaagaag
     6841 gaattctttc gatccgccgt acgctccaag aaaaagggac tgaacctgaa acagactttt
     6901 taaatcggag aatgacgaaa aaacaacaga agagatttgt agtgaaagta atcgaacaac
     6961 ttggttatga ttttagtcgt ggtcgtttag atgacactgt ccacccattt atgactggaa
     7021 ttaatccgaa tgatgcacga atcacgacac gatggaacga aacagatttc aaaatggctg
     7081 tttttggagt gatccacgag gctggtcatg gtatgtatga acaagacatc gatgataaat
     7141 atgcgtatac accggtttat gaaggtacat caatgggtat ccatgaatca caatcgttgt
     7201 ttaacgagat cattattgga agtagtcgca gcttctggaa aaagcaatat cctttctttc
     7261 aagaatgtgc agaaggaaca ttcgatgata ttccttttga caaattttat cgttcattga
     7321 accatacaaa atcaagtttg gttcgtatcg aggcagacag tttgacttac cctctccata
     7381 tcatcattcg ttatgaaatc gaaaaaatga ttttcaatga aggggtagat gttgctgaac
     7441 tacctgaaat atggaatcaa aaatatgaag agtatctggg aatacgacca acaaatgatg
     7501 tggaaggcat cctacaagac gttcattggt caggtgcaag ctttggttat tttccctctt
     7561 atgcattagg gtacatgtat gcggcacaat tacttcattc tttgcagcaa gaaatagatg
     7621 tggaaaatgt tctagcgtca gacgattatt ctccaatcaa aaactggatg tcagagcata
     7681 tccatcaata tggtgcatct agaaaaccaa atcaattaat catggatgca acacatgaac
     7741 cgcttaatcc gcaatacctg atcgactata tgaaagacct gtattttaat gtatatcagg
     7801 taaaataaaa tacaatttac aaagatagct gaaattcatc taatctatag atgttttcag
     7861 ctatttactt aagaaagaag tggaacgaat ggataaatta caagtgatcg ctgattatag
     7921 tatggaccaa ttagcgcatg atcaaacagg acatgggagc gatcatacca aaagagttgt
     7981 caagttagct gaaaaaattt tggagacgga accgcaggca gaccgctttg tgacgcttgc
     8041 agcagcttac ctacatgata cgattgatga taaagtcgtt aaaaatgaga acgaggcaaa
     8101 gcaacagctt cgtgtattcc ttcgctcttt gccaatagta gaggaacaga tctcgatgat
     8161 tttttccatt atcgaaaata tgtccttttc taaaaatctc tctgaagcgg ccgaactatc
     8221 tctggaaggt aagatcgttc aagacgctga tcgtatcgaa gcattaggag caatcggcat
     8281 tttgcgtact gcttatttcg gcggtggaca tgggcacccg atttttgatc ctgaactgta
     8341 tccgcaaaca ttcaaggaca aagaaaatta ccgaaaaggc acgacagtga tcaatcattt
     8401 ttatgaaaaa ctgttcttgc tgtctgataa aatgaatact gattacggat atgaagaagc
     8461 caaacgaaga gaaaccttca tgcgtgaatt cttagaagaa ttttttctgg aatggtctat
     8521 tgacgatgaa aattttactc cagacaattg gatcaaaaat aacaaaaaat gatggaggtt
     8581 gtgacagaag tggtcagctc caagatattt cggatcattt tccgtaaaag ttacttctgg
     8641 aacactgttt accaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt
     8701 tcaaaaaata gcttctcgtg tttgatgaat attgtaactt gtgattcgga attggcagga
     8761 gaacaccgtt tatt
//
LOCUS       DAZUJO010000104         8672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000104 DAZUJO010000000
VERSION     DAZUJO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.109"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..1196)
                     /gene="menA"
                     /locus_tag="ACG8OL_002567"
     CDS             complement(243..1196)
                     /gene="menA"
                     /locus_tag="ACG8OL_002567"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS4818498.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1209..2312)
                     /locus_tag="ACG8OL_002568"
     CDS             complement(1209..2312)
                     /locus_tag="ACG8OL_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS4818499.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2402..3337)
                     /gene="pplA"
                     /locus_tag="ACG8OL_002569"
     CDS             complement(2402..3337)
                     /gene="pplA"
                     /locus_tag="ACG8OL_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS4818500.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3639..5573
                     /locus_tag="ACG8OL_002570"
     CDS             3639..5573
                     /locus_tag="ACG8OL_002570"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS4818501.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            6030..6446
                     /locus_tag="ACG8OL_002571"
     CDS             6030..6446
                     /locus_tag="ACG8OL_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS4818502.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6460..7026
                     /locus_tag="ACG8OL_002572"
     CDS             6460..7026
                     /locus_tag="ACG8OL_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS4818503.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            7049..8035
                     /locus_tag="ACG8OL_002573"
     CDS             7049..8035
                     /locus_tag="ACG8OL_002573"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS4818504.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8190..>8672)
                     /gene="scm"
                     /locus_tag="ACG8OL_002574"
     CDS             complement(8190..>8672)
                     /gene="scm"
                     /locus_tag="ACG8OL_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS4818505.1"
                     /translation="SDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDS
                     STTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSD
                     NRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR
                     "
BASE COUNT         2559 a         1673 c         1752 g         2688 t
ORIGIN      
        1 gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg aaagccattt
       61 tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc ctgataaacg
      121 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
      181 tttttatttg gctgttctta ttacttgatg cagggcagtt ttttcacaac ctcttcatgc
      241 tgttaaatgg cttgcataat gattgttagt aatagcccca atacataact ggcattgaat
      301 aagaccatat ttttgatgga atagctgaaa ctgataggct ggggtaacgt ttgcttgaat
      361 tgatccaaat gcttcttcac aattggcaag gtcaagaagg tgaccaagat cggccattca
      421 tagataccgc tcaacaaccc cattaaaatc acgaaatagc aagcatacat caatagctgg
      481 aataagactg ttcccgcagg acgtccgata taaaaaacta gtgtatagcg atgattttca
      541 atatcacggt caagatcccg caaattatta gccaacatga tattggcaat cgtaaacacc
      601 aagggtaagg aagctaatac gattgctgct atagcccaaa gatttcctac caaagcaaat
      661 tgtccagatg aaaagttgac tgtcagatca tagacatctg aaccgaatgt attgacataa
      721 acgaccatgg caaaaattcc aaggcccata gtcaccccac tgaaaatttc ccctaaaggc
      781 attcgagata aaggaatcgg tccgaatgta tagaatactc caataaaaca acagataaat
      841 cccatcaaaa gtaaaagcca acccgtattg atcgttagaa taacgccgat aacaaaaaca
      901 aatgcaatca tagctaaaat catgtttcga atcaatacag gagatagatt tctgcggcca
      961 atgacatttt cttcatactt atatacttca gaatgagcct tttcataatc catgtaatta
     1021 ttgatagcgg tagtcgctaa atcaaaaact agcattccaa taaaaaataa aaaagtgttt
     1081 agccaatgta tctcatgaaa aaaagcgata gagaataata cccctaccgc aaaaggaaat
     1141 acactagcca cctttgttcg tatttcaact acttctaaaa agatttttag tgtcatggtt
     1201 cgtactcctt attttagttc agaacggtca cccatcgtgt aatgggagtc ctccgataat
     1261 ttgaaggtgt ctttgattgg atcggtcaca tatactttat cgtcttttgt cacgaagatc
     1321 gcttccgtcc cgttattcaa ttcgctttcg acgtattcca gtcctttttt cacacccatt
     1381 gaaaagactg ctgtagacaa cccgtcacca tcaattgatt tatctgttat gatcgtgaca
     1441 cttgcgatat cattgtcgaa cggataaccc gtttttggat caaacaaatg atggtatttt
     1501 ttgccatcta cttctaaata acgctcgtaa attccagagg taactaatgt tttgttccgt
     1561 tctttaatag aacctaaaac agatccgcga gccatgttcg gatcttggat gccgactgtc
     1621 cagtcttggt tttcgcctct tgggctgtgg cctaagacat agacattacc gcccaagtca
     1681 acgatggcag tggtcactcc ttgttttttc aagactttca ctacttcgtc cgtaatgtaa
     1741 cctttggcga ttgcacctaa atcgatgatc attccttttt cttttagata aacagttttt
     1801 tctttgtcat ttagttcgat cttatgataa tccactaatt tcaatgcctg atcgatttct
     1861 tcttgggaag gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt gattgcacca
     1921 atcgccatat cgaatccgcc ttgagaatct tggctatatt cgtaggcccg cttgactaac
     1981 gtgtagacgt catcagaaac ttttactggt ttgatcccag cttgctcatt cacttcatcg
     2041 atctcagaac ctttttgatt gatcgtgatt ttatcaccaa gttcttttac tcgatcgaag
     2101 gcaggtttca atgctgattc tttgccttca tcatacacac ggatacgaac gtaagtccct
     2161 aacaagaact gttcatcaga atatggatct ttcaatatct tggtagctgc tgaactttca
     2221 gtggttgttt tatttgctga gttgctgcag ccagttagca ccataaaaga gatgcccagt
     2281 gtgattaagg aaaatagtag ttttttccgc atctttttcc tctcttcatt ttgaaaggtg
     2341 agacgactgc ctaatctggc tatcgtccca ccctccaact tttattagta tgattatgtg
     2401 attatttttc tgttacgata ttatctactt cgattgtttg agtgtcgcct ttttcagctg
     2461 cattcactaa ttgttgtgcg tacatgatga atgaatgtga gctgtgtgta gcacctgtaa
     2521 caacttctac attagcaggt gagccatctt cttcgcccat tgctttgacg aattcgtcgt
     2581 tcagagtagg gatgtaagtt tttggttctg taccgctttt tgctttcata ttttcattat
     2641 attctgtatc atcttgtttt gatttaccgt ctttgtcgat ataatcaaag tttgattcag
     2701 ttactttgcc gccagcaact gtcatttcga attgcacacg gtagccgtta gagtagttct
     2761 tttcttcaag cttgtaagtg ccgtctttca aatcagcacc attgtcgatt tcgattgtat
     2821 ctgtgttacc agcttgagca gcttggatca attgttgtgc gtagttttgg aagctttcag
     2881 atgagtgagt agctccagtt acgacttcca caccgcttgc gctttgtgct tttaagaatt
     2941 ggtcattcaa ctcaggaatg tatttttctg ggtttgtgcc agattttgct tccatgtttt
     3001 tgttgtattc tgcatctttt gtttttgatt cgccgttttc attgacgtta tcatattttg
     3061 attcagtgat tttgccgtct tttacaacca tttcaaagac tgcacggtaa ccatttgaat
     3121 aatttttttc ttctaattta tatgtgccgt cttgcaaatc tccgccagct acaacttttg
     3181 cagatgattc tgttgtgctt tttgcagtcg aagattgtgc tgtagatgat tcagaagaag
     3241 agtttgagct tccgttgtca tctgcgccgc aagcgcctaa cactagagct gaaaatgcta
     3301 atacggcaaa tccagttaca aaacgttttt tcttcatttt aataccccac cattccattt
     3361 ctagtccaca atttttcttt gtgattcatt atacattcta atctttttag cctataagtc
     3421 aactattttt gataaataac ccatgtaata tttcaaacgt tttctttcag caaatttcgt
     3481 gaaaaaacgc tttattttaa aatttgaaaa aaatccaaaa ggagttagac aaaagaacag
     3541 aacatctata taatatatgg agattgtgta ataagtatca aactaaaaga attatcatat
     3601 attcattata tagatagtct ataaggagcg gatgatcaat ggcaaagcaa aatattgtcg
     3661 ttgtaggagc aggatatgcc ggtgtttcag ctacaaaata cttggcaaag aaactgaaaa
     3721 aagaagatgt agcaattact ctgatcgatc gtcattcgta ccatactatg atgaccgagt
     3781 tgcatgaagt agctggcgga cgtgtagaac cagaagcaat ccaatacgat ctgcagcatt
     3841 tgtttgctcg tcaaaagaat gtacaacttg tcacagacac tgtaatcggt atcgataaag
     3901 aacgaaaaat cgtcaagacc aaattaggtt cctatccttt tgatcagctg atccttggaa
     3961 tgggcggaga gccgaatgac tttggtacac ctggtgtcaa agcacacgga ttcacccttt
     4021 ggtcatttga agattctgta aggattcggg aacatattga gaagacagta gcaaaagctg
     4081 cattggaacc agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc tgtggttctg
     4141 gatttaccgg tattgaaatg attggggaac tgatcgactg gaaaaatcgc ctagccaaag
     4201 attataaatt agatccaaac gaaatcacac tgatggtcgt agaggcaatg ccgacgatct
     4261 tgaatatgtt ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt aagcacaacg
     4321 tggaattatt attgaatgca ccaatcgtag aagtggctgc tgatcatatc aaattgaaag
     4381 acggacgcaa cattccgact cacacactga tttggacagc tggtgtcaaa ggaacttctg
     4441 acgctgctga ctttggatta gaatcagcac gcggacagcg gttgatcgcc aatgaatata
     4501 tgcaggcaaa aggctacgaa gataaaaaca tctatatcat tggtgattta gtcttttatg
     4561 aagaattccc aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa acaggacaca
     4621 cagccgcagc gaatatcgtg gcttcgatca aaggcggaga aaagcataaa tttaaaggaa
     4681 actatcaagg atttatggtt tctattggtg ccaaatgggg ggtagctaac ctatttgata
     4741 agatccattt aagcggattt ttagctatcg taatgaaaca tatcgtgaat ttgaaatact
     4801 tctttgatat tcgttctggt tattatatgt tccaatattt aatgcatgag tttttccata
     4861 tcaaagatga tcgcaacgtg actcgaggac attcttctcg ttacggcaat gtcttatgga
     4921 gtgtgccact tcgtattttc tatggtttag tttggttcgt tgaagcttcc aaaaaaatca
     4981 ttgggaatgg tgaagtgttc aaaccaagta cttggtttgg tgaaggatca tggttcactg
     5041 gaaacagtgt gttcccttgg acatgggaat acaatgtaac agatgttgcc agtggtgctt
     5101 ctgctgctgg tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc ggagaagcag
     5161 cgactcaagc tgcacatttt ggtttgagtt atgcatacgg tgaagaacca atggctgtga
     5221 ttgagaagtc accagactgg ttcacaaaga tcatgcaagt gatcatgcca aacaatgatg
     5281 tagcaatgtt cttccaaaaa ttcatggtat tctttgaaat tgccttggca ttagcattga
     5341 ttgctggatt attcacatgg ctatgcagtg cgacaacgat tgtattggtg atcacgttct
     5401 gcttatcagg catgttttac tgggtaaaca tttggttcat ttttgttgct ttcgcattga
     5461 tgaacggttc cggacgtgcg attgggttgg atcgctgggt aattccatgg ctacaacgta
     5521 cactaggtaa ttggtggtac ggtagaccaa gttcccagta cggcaagaaa taaatagata
     5581 aagaacgtta tttgaatgca agatttcaat agaaaggggc tgagacaaac cgtcacagcc
     5641 tcctttttat gtaaaattaa aggaataaga tgcccgagat aagcctccat gagcaagaca
     5701 caggtgtcac aacctctgtt acaacctctt gttataaacg gtgttccaga agtagcttct
     5761 tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa attccttctt
     5821 atttcttgaa gttgcccact tctgtcacaa cctcttgtta taaacggtgt tccacctgct
     5881 gcttctcggt aataagccca aagaattaga aaacatggaa agctgttttc tattcttcgt
     5941 tcttattact caaagcaaga cacaggtgtc acaacctctt gtttttatgc taaaattggg
     6001 tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa agaatttgct gaaaaaagtc
     6061 tgctcaaacc ttgggatgtt tttattattt tgcttttgac tgttctttct tttttaccag
     6121 tcatgatttt tggtcttcag caaaacaccg caacagcaga aaaagaagcg gtactgcgtg
     6181 tggatggcac agaaatcaaa acattccctt tgatggctgg agaagagagc aacacatata
     6241 cttacaaaga tgaacacgga gattataact tgatcgagat agatggcgat cgagtacgga
     6301 taaaagaagc agattgcgat gaccagatct gcgtacgtcg aggctgggca acgaaaaatg
     6361 gcgaaaccat cgtctgtttg ccacataaat tagtgatcga agtccgttca acagatggag
     6421 gtgacgaaga cagtctgatt tattagacct ttacacacca tgagtaaatt acaaaaaatg
     6481 atttatattt cgctacttgt tgcacaagga gtgatcattg gtttattgga aaatatgatc
     6541 ccttatcctt tttcttttgc gcctggtgca aaactaggct tagccaatct aatcacgatc
     6601 gttgccattt ttaccatgaa aaaacaagac agcttcttac tactttggct acgactattc
     6661 ctgactactt tgctaggcgg aacgatttcc acattccttt atagtatgag tggtgctttg
     6721 cttagttatt ttggaatgtt attggtcaag caattaggac caaaacgtgt cagtatcatc
     6781 ggaattagtg cggccggcgg tttcatgcat aatgtcggac agcttttgat ggcttctttc
     6841 attgcacaat catggacagt catgttgtac ttgccagtcc tttcagtttt agggatttta
     6901 tcagggattg caatcgggat cgcagcgaat tatcttttac gacatgtgca gacacttcaa
     6961 cgattccaaa aagattatga gcagggaacc aaacaaaaat ggagcccaat gtttaccaaa
     7021 aaataatcgt cacacaagga gtttttttat gaagctacat ccaatgtggg aaaactatcc
     7081 tgctttaagt aaagaattga cagaaacact aaaacggatg gaagaaactg tccgcctgaa
     7141 aaacaaacct gtcgaacaag caatcaaaga aacgatccat gccggcggaa agctgttgcg
     7201 tcctgcctat cagctattgt tctcacaatt tggaccagag caagatcgag acaaagccat
     7261 cgctttggca gcttctatcg aaatgctcca tacggccaca ctgatccatg atgatatcgt
     7321 tgatgaagcg gatctgcgac gtggtcagcc taatatccgt agtcagtttg gtaatgtcgt
     7381 ttctgtctat gcaggagact atttatttgt ctgctgtttt aaactattgt ctgattactc
     7441 cacttccttg aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat tgaatggcga
     7501 attagggcag atggatgatc gctataatta tgaactttct gtaaaagaat atttggaaaa
     7561 tatttcaggt aagacagctg aactgttcca attaagttgt tctgttggcg cttttgaaag
     7621 tggaacaagc gagcgcttcg caaaaaaagc aggagatatc ggactttcta tcgggatggc
     7681 ctttcagatt atcgatgata ttttagatta tacaaaagag agccaggaaa tcggcaaacc
     7741 tgtactagaa gacatgcgtc aaggggtcta ttctttaccg ttgatctact ctttgcaaaa
     7801 aaacaaacca aagctactac cttatttaga gaagaaagcg gcaatgacag aagaagatgt
     7861 cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta ggaagttagc
     7921 ggcctcttac acagaaaagg ctttgaagga aataaagaaa ttacctgcca cctctcttcg
     7981 aacaaaagag aatctctttt cccttaccca actgattttg gatcgcaaag actgaatagg
     8041 gaaaggttcg atacgtaaat aaatttctca gaaggaaatc tacgtgtttt ttgacaaatt
     8101 gaattgtagt tcttgtattt atgataaaaa gcaaaaataa agagcaacgt ccgttagcga
     8161 cgttgctctt ttttatcttg aatatttttt taccttttcg atttcttata actcataaaa
     8221 gtgattctta aaccgattac aagtaatata acgcctgcga gtgttatcca attattcgat
     8281 tgacttccag ttttaggaag tgctcttttt gatttgtttc cattgtcatt gcttgtttta
     8341 ttagttaagt taaagttcgt cctgttatcc gaacttgttt ttgagaagga gacacttgca
     8401 tgacgtgtat cgcttgtggt agacgacgtg tctgtactat cgcttgaagt tgatgaatct
     8461 gagctatcac ttgtggtaga ggatgtatct gtactgtcac ttgaagctga cgtgtccgtg
     8521 ctatcacttg tagtggtaga tgaatctgag ctatcacttg tggtagaggt agtatctgta
     8581 ctgtcacttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     8641 tcacttgaag ctgatgtgtc cgtgctatca ct
//
LOCUS       DAZUJO010000105         8493 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000105 DAZUJO010000000
VERSION     DAZUJO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.110"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8OL_002575"
     CDS             complement(<1..149)
                     /locus_tag="ACG8OL_002575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4818506.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8OL_002576"
     CDS             complement(593..1714)
                     /locus_tag="ACG8OL_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS4818507.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8OL_002577"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8OL_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS4818508.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8OL_002578"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8OL_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS4818509.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8OL_002579"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8OL_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4818510.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8OL_002580"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8OL_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS4818511.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSIPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8OL_002581"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8OL_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS4818512.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8OL_002582"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8OL_002582"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818513.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1727 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggaattga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DAZUJO010000106         8312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000106 DAZUJO010000000
VERSION     DAZUJO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.111"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8OL_002583"
     CDS             complement(105..629)
                     /locus_tag="ACG8OL_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS4818514.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8OL_002584"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8OL_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS4818515.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8OL_002585"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8OL_002585"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS4818516.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8OL_002586"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8OL_002586"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS4818517.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8OL_002587"
     CDS             3157..4026
                     /locus_tag="ACG8OL_002587"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4818518.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8OL_002588"
     CDS             4052..4567
                     /locus_tag="ACG8OL_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS4818519.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8OL_002589"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8OL_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4818520.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8OL_002590"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8OL_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818521.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8OL_002591"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8OL_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818522.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8OL_002592"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8OL_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4818523.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8OL_002593"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8OL_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS4818524.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..>8312)
                     /locus_tag="ACG8OL_002594"
     CDS             complement(7873..>8312)
                     /locus_tag="ACG8OL_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818525.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         2374 a         1714 c         1500 g         2724 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt ga
//
LOCUS       DAZUJO010000107         8249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000107 DAZUJO010000000
VERSION     DAZUJO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.112"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACG8OL_002595"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="ACG8OL_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS4818526.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACG8OL_002596"
     CDS             complement(1804..2688)
                     /gene="ftsX"
                     /locus_tag="ACG8OL_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS4818527.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACG8OL_002597"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="ACG8OL_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS4818528.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="ACG8OL_002598"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="ACG8OL_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS4818529.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACG8OL_002599"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="ACG8OL_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS4818530.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACG8OL_002600"
     CDS             complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="ACG8OL_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS4818531.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8134..>8249)
                     /locus_tag="ACG8OL_002601"
     CDS             complement(8134..>8249)
                     /locus_tag="ACG8OL_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFS4818532.1"
                     /translation="KILIIDDVYTTGQTLFHAADCLQNAQPKIIRTISLAR"
BASE COUNT         2200 a         1816 c         1440 g         2793 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt tcgttgttat
     7441 ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt acggcggtaa
     7501 acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg tcccagcatg
     7561 ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg tttcgtacgt
     7621 acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa agcagcagca
     7681 tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt gaatttgcgg
     7741 atttggcgtt ccaatttgtc aacaaccaaa tcgatacttg cgtataaatc tggagaagtt
     7801 tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt tgctgttttt
     7861 tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact gaaatagcgt
     7921 tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac ttcgatattt
     7981 tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca caatcaagaa
     8041 ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta ggttatttgc
     8101 aaattaaatc gctatcaaat ttccacatct gattcatctt gccaaagaaa tggttcggat
     8161 gattttcggc tgtgcgttct gcaagcaatc tgctgcatga aaaagcgtct gtcctgttgt
     8221 atagacatcg tcaataatca atatttttt
//
LOCUS       DAZUJO010000108         7932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000108 DAZUJO010000000
VERSION     DAZUJO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.114"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2671)
                     /locus_tag="ACG8OL_002602"
     CDS             complement(<1..2671)
                     /locus_tag="ACG8OL_002602"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS4818533.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2658..3020)
                     /locus_tag="ACG8OL_002603"
     CDS             complement(2658..3020)
                     /locus_tag="ACG8OL_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818534.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            3239..4459
                     /gene="sstT"
                     /locus_tag="ACG8OL_002604"
     CDS             3239..4459
                     /gene="sstT"
                     /locus_tag="ACG8OL_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS4818535.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4606..5241
                     /locus_tag="ACG8OL_002605"
     CDS             4606..5241
                     /locus_tag="ACG8OL_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS4818536.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5432..6172
                     /gene="truA"
                     /locus_tag="ACG8OL_002606"
     CDS             5432..6172
                     /gene="truA"
                     /locus_tag="ACG8OL_002606"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS4818537.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6317..7930)
                     /locus_tag="ACG8OL_002607"
     CDS             complement(6317..7930)
                     /locus_tag="ACG8OL_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818538.1"
                     /translation="MDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLSRIIGLDNV
                     WSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVS
                     YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYI
                     RDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVL
                     TFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLG
                     LIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSL
                     FIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAV
                     AIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNT
                     VLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYL
                     SIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2625 a         1234 c         1489 g         2584 t
ORIGIN      
        1 ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca tattgtatca
       61 gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct tggataattt
      121 gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag ctttctgtag
      181 gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca tagtaaggag
      241 ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga gcaacaactt
      301 ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg aaataatctg
      361 tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt gcgggtaaat
      421 ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct tggatgaatt
      481 tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat tcttttcttc
      541 tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag taatcatcat
      601 aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttct gtattgcttt
      661 gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg ctttgttcta
      721 ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg tcaaaaccaa
      781 atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg atggctattt
      841 tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact agctgggtat
      901 aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt ccttcccatt
      961 ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct gttccttctg
     1021 ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta gggacacgtg
     1081 caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt aaccagtcac
     1141 ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt ccattaattt
     1201 tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg tttaggaatt
     1261 gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc ttgtttactt
     1321 taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa aagacacccg
     1381 aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga atcttcaagg
     1441 aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat aggagaagga
     1501 tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct gacaatccgg
     1561 tcagtatttt taaatctgaa ggaaagacag gctcttcccg catttccatt ttgattactg
     1621 tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac caaaaacgat
     1681 tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc aggatcatgg
     1741 gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc caaccttctt
     1801 ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct gcaactaaaa
     1861 aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga gttaattgtt
     1921 caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt ttttgtatat
     1981 aaaataaaaa gataactagg agggacactg ctaataaata gcaaataaaa aactgcccga
     2041 tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat gtagatacgc
     2101 gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat acagcaagaa
     2161 aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat aaaaacgaag
     2221 agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc gctgtatctg
     2281 tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg aataaaacaa
     2341 tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag ttccattctt
     2401 tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt ccattctgta
     2461 atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt gttaaaaata
     2521 atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact gtataaggaa
     2581 tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga ctgataggtg
     2641 ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa aaaaaagata
     2701 ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata atgtaaaact
     2761 tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa atttgcacca
     2821 ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt tgttgcatat
     2881 gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat acaagcgaca
     2941 aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa acacctcaca
     3001 aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt aataacatcc
     3061 taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa
     3121 tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt tgcataaaat
     3181 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     3241 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     3301 ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct tgaaggcaat
     3361 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     3421 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     3481 tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     3541 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     3601 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct gggctgtatt
     3661 gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg atcagatatc
     3721 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     3781 attggtttat caatcaattg ctacgactgg aatagctgga ttggcagagt atctgcagct
     3841 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     3901 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     3961 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     4021 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     4081 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     4141 agggattgaa gcaccttttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     4201 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     4261 gtttggtatc tctaatgatg tcgcgatgca aatcgtcggt attggattta ttatcggtgt
     4321 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     4381 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     4441 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     4501 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgctt
     4561 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     4621 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     4681 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     4741 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     4801 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     4861 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     4921 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     4981 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     5041 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     5101 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     5161 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     5221 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     5281 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     5341 ctgttttaga gaagaaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     5401 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     5461 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     5521 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     5581 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     5641 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     5701 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     5761 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     5821 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     5881 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     5941 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     6001 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     6061 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     6121 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgat aaaaatactt
     6181 tgataatgga tgaaacgcat gtttcacagt ataaatatcg atgttaaata atgtatgaag
     6241 caaattaaag aaatataaaa aaagactttt tagcctgttt atgtgtacag ctaaaaagtc
     6301 tttcttaaat aaaaatctag tgagaatgtt gtacttctct tctcggttgt ttttgtttag
     6361 aaaaagtaaa tcgataaagt aaaaataaaa tcaaagtaac gatagataaa tatcttgaag
     6421 caattgctag ttttgtgtac tgataagtaa taacgacttt atttatacct ggaggaatag
     6481 ttaattcagt tgtgccaaac ttagataatt ttgtttgtac tagtttgcca ttaatagaag
     6541 ccacttgccc tttataacga taaataggtg tcgtcaagac agtattttta ttattcgaat
     6601 tatctaattc aattgtaagt ttgttactca taaaatgagt tgttggattt atttcctggt
     6661 tacctagtaa atattgatca ttattaatca tatcaggatg atagattgac tctttgtttg
     6721 cataatcagt atgattgtaa ttttcagcta tagcaacagc gtcttcaggt gctataagtg
     6781 ttaatgaatc tttgttagca tggatagaat tgcttactcc tgagagatgc aatattagtg
     6841 caagaaacgt aaagaccatt attttccatg aattattttt agtagaaaaa atataataga
     6901 tcactatact catcgtatag gctataaata aagaagaaaa accatttaac cgccagacaa
     6961 attgtagact cttaactggt gtatggttaa aaagttgcca aggaaataaa ttggttgaac
     7021 aaaaaagaac aaatacacca agaaagaaaa taaataaatc atcagcagtt aattttttta
     7081 taaaaataaa agcaccaatc aatccgagaa ataagaataa tccaatagta tagttgttat
     7141 agttattatt taaaatgtgt gtcagcatat cactaggagc cataccactt aattcttttc
     7201 ccagtgggac tttcaattct tgtgctctag attgttgaat aaaaggaatt aagaatcccg
     7261 cacttaaaaa aaaggtgagg acggtagctt ttaaaagact taagagtcta gcgatttttt
     7321 ggtcccaaaa ataaaaagaa agaaacaacg ttcctccaat aaatacagaa accatagcga
     7381 cagaaagtaa atgagtatac actactagcg tcatgccaat agacaaccag taccattttt
     7441 ggtaatctct aatataaatt tcgtaacacc ccattagtat caaaggcaaa aatgttaaag
     7501 cgacagcttc tcccaatgaa gcacgatgga atatgtcaat ggcacgatag gcggaaaatg
     7561 tataaataat cgaaaataac agcgaaatat aacgattgtt ttttatttgt ttcatagaaa
     7621 aataagaaac gaccattgtt aaaaaagtga taaaaaaata gtagatatta taacttaaaa
     7681 caagatttcc catcatttta taaaataaaa aagctggata aagagtaagc catggataaa
     7741 aaatattcat cattgttcca tgatgatcga agttattgaa gtttactgga gaactccata
     7801 cattatctag tcctatgatt cgagataaat gaaagtaagt atcctgattg acactatgat
     7861 agattcccat agacatgaat agagggatga cgtaagcaaa acttagaaat aacataaaaa
     7921 tgatatctat ta
//
LOCUS       DAZUJO010000109         7825 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000109 DAZUJO010000000
VERSION     DAZUJO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.115"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1183)
                     /locus_tag="ACG8OL_002608"
     CDS             complement(<1..1183)
                     /locus_tag="ACG8OL_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4818539.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQ
                     YRQLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQ
                     DLLTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLEN
                     LH"
     gene            complement(1362..2135)
                     /locus_tag="ACG8OL_002609"
                     /pseudo
     CDS             complement(1362..2135)
                     /locus_tag="ACG8OL_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2171..3358
                     /locus_tag="ACG8OL_002610"
     CDS             2171..3358
                     /locus_tag="ACG8OL_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFS4818540.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            4091..5059
                     /locus_tag="ACG8OL_002611"
     CDS             4091..5059
                     /locus_tag="ACG8OL_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4818541.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            5127..5885
                     /locus_tag="ACG8OL_002612"
     CDS             5127..5885
                     /locus_tag="ACG8OL_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS4818542.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKKIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            5953..6663
                     /locus_tag="ACG8OL_002613"
     CDS             5953..6663
                     /locus_tag="ACG8OL_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4818543.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            6699..>7825
                     /locus_tag="ACG8OL_002614"
     CDS             6699..>7825
                     /locus_tag="ACG8OL_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS4818544.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         2599 a         1238 c         1589 g         2399 t
ORIGIN      
        1 tatggagatt ttccagtcgt gcattcgaat acttcttcag taatgccagc cgaatccctt
       61 tctcgtattt gaaaaaggct tttttgatat ggtgcagttc caatggaact tctccaggca
      121 ttgctcgaag cagctggaaa aaagtatctg gatctttttc ctgatacgca gtaagcaggt
      181 cttggtagta acgataagct gttttaagtt caggagaaat ctttagaagg cggtcaacta
      241 cttctgtttc agtcaatagt ctccaattga aattgcgtct tttcgttagt gtattgtaat
      301 ctaaacgatc ctctgatttt aaaagtaatt tgaataagga tttcaattgg cgatactgtt
      361 tagcttgtat tgggtctttt tgatcaagtg ttttcatcac acgttttcga accgcatcga
      421 agaagcggtt cagatgttga acaatgtgaa accgatcgac gatcacttgt gcatttggaa
      481 aacactcttt aactagtgag aaataaggag cgttcatatc cgttacgatg taatgtacac
      541 ccaacctcgc tttgagagaa aaacgcatga aaaaagcaac gagatctttt ttacggcggc
      601 taggtaagat ctcaaacagt ttcccggtct caccgtccat acaagaaaaa gaaaatttac
      661 cgtgccatgt agaaagagaa cgaaactcat cgaataatag acaccgaggt aaatactcaa
      721 gattctgacg gatatctgat gacaaggcgg acaatgtccg ctgaacagtt gtcacagaaa
      781 catcgcaatg tttaccaatt gcttttatag attgacgctc tgtcaattct tctaagatct
      841 tgtaatgaat ctgcttagaa ataaagcaga atggggtaac gagatacgtc cgagcggtaa
      901 aatatttttt acatgctcgg cagaggtatc gctgtttctt caaccgaaga taggtccgtt
      961 tattaccgct acgattcagg agaatcatag agatcttctt gccattttta acgatttgct
     1021 ttgtagaatc tttacaacac ggacaacgtc tgtaggtacg gatcagagtt ccctcaataa
     1081 cgatactttt tctgctttta taaaaagttt cttcaaagga ttgaatttgg atatttgagt
     1141 ctttaatagt aaagagttgt ttgataaaat gattcatgga catacaagaa ttcccccaga
     1201 atgatgttgt aggagactct attctaccgg aaattttgta tgtctttttg tttttttaca
     1261 aaaaaactag gacagataga aaatttccat cagtcctaga aattatagag ccaataaaac
     1321 ttagtccgat atgcggcgcg ttctgtgcgc cgcatatttt tttagttttc aaagatcaga
     1381 ccttgttgta aatagatccg tgcccgaaag ttataaaagt tgcgataacc aaacgcaatt
     1441 cgtttaatca gcttgatctt gttatacagc ccctcagtga cgccgtttga gtgggacacc
     1501 ttaaaggcat ttgaaatacc cgattggtag cgatggaaga tcgtgaattt ctttttaaac
     1561 gactgaggta acgtagatga aattgtttta agggtttctt gaaagcctac cgtatctcgt
     1621 ttaacgaaat aatagcgtaa taattgacaa aaatgatacg cagttcgcaa ttcttcgttg
     1681 tagcttaaca gctcattaac gatttctgtt tgactaattg gtcgtttaaa cagtcgatga
     1741 taatgccgtt tgagtgcctc ccatgtcgtg gaatctttga gtaagagttt ccaatatcgc
     1801 ttgagccgac ggtacttctt ctgattttca gagtgtgagg ttcgaaaacg attcatgatc
     1861 tggacacgta attgattgaa gctgcgattg atgtgttgga caatatgaaa gcggtcagtt
     1921 acgatctccg catttggaaa gaccgtttta agcaactgac aatagctggc attcatgtcc
     1981 atcactaggt acttgacctt taaacgggcc tttctaggaa agcgcaggaa gtattgagtt
     2041 agtttgtaaa gtctgcgatc ttcaagggtc gtaaatagct gattggttgt cccatcaaca
     2101 caaataaaac tcatggaacc agaagtggca acattgtgta cttttctttg caatcttgtg
     2161 tacaaaacta ttgcaaaaca cagcgcataa gaatccttac aaaacagtag atgaagcaac
     2221 ccaattagat cttgttatag ttgttaatca gctcttaacg ggttacgatt caaaatgggt
     2281 caacacccta tacttagata agtcacttga atatcagaac ataattcagg ctttttcacg
     2341 cacaaatcgt cttaatggtc ctgaaaaacc gttcggaacc attcgttatt atcgtaaagc
     2401 acatacgatg gaacagaaca ttgaagaggc tattgagtta tactcaggca aacaagcaaa
     2461 agggttattt gttgaaaaac tatataataa cttagaaaag ttgaacgata tattcgaaga
     2521 aattaagtcc gtatttaata tttctggcat agaaaacttc agtcatttac cggaggttcc
     2581 aggggaaatt aagcaatttg taaatcttta taatcaacta atccattgtt atgaatcagc
     2641 gatgatacag ggattcacat gggcaaaatc gacttatgaa atccaggatt atgaacctat
     2701 aacagtagta atcacagaat atgaatttaa tatcttggca caacgttaca gcgagataga
     2761 gagagaacct agagacggaa atgatagtat agcgtatgac attgattacc aattacctga
     2821 aggtagtcgt agtcgtatta attatgagta tttaaacaca aacttcaaaa aatacatgaa
     2881 tataaaagaa gaacgtgatt cgaatttaat tcaatcattg ttaaacgact tacatcgcac
     2941 atttgcgcaa ttaccagtgg agttacagcc atatgctgag atgattctaa atgatgtaaa
     3001 taatggggaa ttagttatca aagaaggatg ggaatttact gattacctca atgaatatca
     3061 gcttagtgaa gaggacgatt ttatccaaga tttagtggat tcaaccaata ttaatgaggc
     3121 attattacgt gagatgttgg atttacgctt aaccgaggta aacattaatg aatatagtcg
     3181 atttgataaa ttaaaatcaa gtgttaacgt aggtcatttc agtggatata tcgagaaaaa
     3241 tttaggtaaa atggtaatac caatcaaggt aaatatgcta attgacagat tattgcgtgc
     3301 atttttatta gaagatgtat tcgatgttac agagtacata aagaattact ttaactagag
     3361 tacacctaca tcttatagta cataaaaaaa ttcatctttt ttagtttaat aattcaccaa
     3421 aaaggtgagt aatgccattt ttgaaaattg agcttgtttt atgtctacat ctgtatccgt
     3481 atataatcca aaggattata aactcagaag agtctttatt ttttctgaat ataactactg
     3541 tatcaaaaac aatcgattat gagacaaaac aaaatgacct cttttattta agaaaggagg
     3601 tcattttttg tatcataaat tgtattaaaa tcaatgtatc actttttaat caattttaag
     3661 accataaatt gcgtactaaa agaaattctg caattcaaaa taaagcattt taagccattt
     3721 tcaaatttaa gtgcacccat ctacccgtca aatcacttta ttttccactc cagtagtttc
     3781 ctaattcaac tgtgtgcaaa acgatttctt ctaaacaaat caagacaaac ttataaacct
     3841 tcatcccagc accataatta tttaaatcat aggcattttt cgaaattagt atatgtactc
     3901 aagtttcact gcagattatt tatatttttt agagaaataa atttttccta ttgacaaaca
     3961 caaagaaagc ggatacaata caattatgtc acatgacgtt atatgacgtt atgtaatatc
     4021 tttacattta atcagttact tttattttgg ataaaattaa catttttaaa attttaagga
     4081 ggaaatgttg atggatgtag caaaaacggt taacgcaatt gtagaaaaaa atgatggaga
     4141 gatttctgga atttatttgg tggcttgtgg cggttcgctg gtagatatgt atgtctcaga
     4201 ttattttctt aaagccgaag catcgaagtg tattacaggc ttatatacag cgaacgaatt
     4261 taaccatgta accccaaagc aattagatga taaatctgtt gtcattctat gttctcatgg
     4321 aggaaataca gcagaaacgg taaaagcagg agaagtcgct caagctagag gagctgtgac
     4381 tgttggtctg actcataata aaactgcgga tttattaaag gtttctgatc atagtttcct
     4441 ttacgaatgg ggagatgatg caaaaatagt caataatcct atggccatca ttcttgattt
     4501 aactgtatct ttggtcaatg ttaccgaaac atttgaacat tacggtaaat tcagagaagg
     4561 catgaaggtt atcgataccg taattgaaaa tggcattaag caggtgcagg atcgtattaa
     4621 agtgtttgct gataagtaca aagatgaaac aatgttttat atcttaggta gtggaccatc
     4681 atatggacat gcttatggtt tttcaatttg ctctttgatg gaaatgcaat ggcttgattc
     4741 aacttctgtt cattctggag aattcttcca tggacctttt gaagtaacag acaaagatac
     4801 gaattttatt ctcttgatga gcattggacg cactcgtgct ttagatgaac gggcaaaacg
     4861 attccttgat aaatatgcag aaagagtaga aatcgttgat gcaaaggaac taggattgga
     4921 tatgatatca gatgaagttt ctgaattctt caatcccatt ttattctaca gtattctttc
     4981 agaatatcgt tcagctttag cagatgcacg caatcatgat ttagatgttc gtcgctatat
     5041 ggggaaagtg gagtactagg aggattaaaa tgaaggtatt aggcttaggt gataacgtcg
     5101 tcgataaata tgaaaatctg ggaattatgt atcctggagg aaattcattg aattttgcag
     5161 tttttgcaaa aaaactggga catgaaagta gctttatggg agtttttggc tcagatgagg
     5221 aggcagctca tgttttatca gcaataaaaa aaataggact ggacaacagt cactctcgct
     5281 ttgaaaaagg cgagaatggc tgtgccagag tgaagattga tgagggcgac cggattttcc
     5341 ttggcagcaa ttcgggaggt gttactcgtg aatacccgat tcagcttact gaagaagaca
     5401 gagaatattt aaaccagttt gaattgattc atatgggatt atatagccat gtcaatcatc
     5461 tattagaaga gttacaaaat gtttctggaa agatttctta tgatttttct gacgacttta
     5521 cggaagaaga ggttcagcgg gcaattgata aagttgattt tggcttcttt tctatagaag
     5581 acttttccga tgaagaagta aagcagtttt tgatgaagca tttttgtgag cgaaatgatg
     5641 tattgattgc aacccgagga gaaaaaacgg ctattggtta tgatggagca aagttttatg
     5701 aagtgaaccc cgtgctagaa gaaccagtcg atacaatggc agcgggagat tctttcttaa
     5761 cagctttcct actttatttt ctcgaagggg acgatatagt caccgcgatg aaaaagggga
     5821 atgaatttgc tgggaagtct tgtctggttg acggttcgtt cggctttggt attcattacg
     5881 attgagcttg ttttaaattc ctgcatttga taacctaaag aaagaaatcc cggtaaagtg
     5941 gaggttaaaa aaatgctgga tacaaatgca caattaccat tgtacttaca attaaaagaa
     6001 atattaaaaa ataaaatcaa tacaggggag tatcccgaga atacgaaaat tcccgctgaa
     6061 tcagagctga tagaaagtta tgaggtcagc cgaatcacag tgagaaaagc agtagaagaa
     6121 ctggtagaag aagggtacct acttaaaaaa caagggaaag gaacttttgt caaccggcat
     6181 aaagtttttc gtaaaattga atatgttatg ggtttcagtg agagttgtaa atctaatggt
     6241 tttacaccga ctagcattgt tttggaacgc aagatcattc cagcaactga agggatggca
     6301 aaaaaactcg atatcgctca aggtgatgat gttttatata tccagagaaa gcgaatggcg
     6361 gataacatcc ccattatgtt agaaaacaat tatttttctt atgaaagata tagtttctta
     6421 atggaggaag agctgactga ttctttgtat gaagtactta aagtccaagg taccttagcc
     6481 atcaatcctg gggaaactgc acttgaaatt gtcacagctg atgaccaaat tgccaaaata
     6541 atggaagtag ctgttgggac gccctttttc tttatggata ctttaatcaa cgatcaaaac
     6601 aacataccaa ttcatgtagg taatcaatat tacctgggag aagtatatcg tttttactta
     6661 tgatttcctg ttttgatttc aaggagtaga ttttatttat gaagcaaata ttgcggtcaa
     6721 aaatgatttt tgatggagag cagccacctt tcaaaggtta tattgtaatc aatgaaggga
     6781 tcattgaaaa ggtggaaaac aactggaatt acagtgatct attaaatgaa aacacggaat
     6841 taattgctta tgatgatcaa ttcgttatgc cagcaattca tgataatcat gtatttttca
     6901 gtggttattt ggctatgaat gctggcatag atttaagcga tacatcttca gctaacgagg
     6961 ctgtagagcg tattaaagct gtaatgaacc ataaaaaagc agatgaacca atttatgctc
     7021 atggctggga tgctgccctg tggaaaactg acccatcaga agagctattg aatgaaataa
     7081 accactttgg tccaatcaca gcgatcgaca aaaatcgcag ccattgttgg atgaatgcta
     7141 ttgctaagca gacttatggt tttacagaaa aagaaaccag tgctgaaagt cgagtattat
     7201 taattaagga aatgctagca aacaaaaagc ttttagcaga ggtttacgct gaatttgaag
     7261 cattactgct ttcaaaaggt gttatatcaa tgaaagaaat cgtttttgat gacgcagaat
     7321 ttctttctaa tatacctcag aggaaaatac ttagtaacct atatgtgcag gcagtccaag
     7381 aacctctaca gcatgaactt ctatatgagt ataaggaaaa agttttccct gaaaaggtcc
     7441 gctttggtgg agtgaagatc atggtagatg gtgttgttgc ggatagaagt ggagatattt
     7501 acggtgttta tcagtcagga gtagcttcac ccgagattga ctatgaggca attaaagcag
     7561 aagttgctgc attcaatgaa gtagagattc cttgctgttt gacaacggaa ggagataaag
     7621 cgggttataa agctgcgtca attcttgtcg aatttggaaa gcggttacct gaaggcgttt
     7681 ttaattcgat tagtgatttg gaaatggtta ctcaaaagac agcagaaaaa atgaacgaag
     7741 gacaagtagt agcagaggta tatccacaaa ttctcggctt gaatcaaacg ttagaagagg
     7801 cttacatgtc aactgttata aaaaa
//
LOCUS       DAZUJO010000110         7616 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000110 DAZUJO010000000
VERSION     DAZUJO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.116"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..743)
                     /locus_tag="ACG8OL_002615"
                     /pseudo
     CDS             complement(45..743)
                     /locus_tag="ACG8OL_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            822..998
                     /locus_tag="ACG8OL_002616"
                     /pseudo
     CDS             822..998
                     /locus_tag="ACG8OL_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1174..1743)
                     /locus_tag="ACG8OL_002617"
     CDS             complement(1174..1743)
                     /locus_tag="ACG8OL_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS4818545.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1817..2140)
                     /locus_tag="ACG8OL_002618"
     CDS             complement(1817..2140)
                     /locus_tag="ACG8OL_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818546.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2294..3106)
                     /locus_tag="ACG8OL_002619"
     CDS             complement(2294..3106)
                     /locus_tag="ACG8OL_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818547.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3433..4308
                     /locus_tag="ACG8OL_002620"
     CDS             3433..4308
                     /locus_tag="ACG8OL_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4818548.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4322..4645
                     /locus_tag="ACG8OL_002621"
     CDS             4322..4645
                     /locus_tag="ACG8OL_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818549.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4642..5394
                     /locus_tag="ACG8OL_002622"
     CDS             4642..5394
                     /locus_tag="ACG8OL_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS4818550.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVCLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5384..7555
                     /locus_tag="ACG8OL_002623"
                     /pseudo
     CDS             5384..7555
                     /locus_tag="ACG8OL_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2265 a         1507 c         1375 g         2469 t
ORIGIN      
        1 aatagttcaa cttttggggt tcagttcaaa gaggggggga ttttttatcg ttgtggaata
       61 tttaaatacc aacgcttgtc atgaaaatct tgtgctccac ccttagtatt tccttctgga
      121 tcgtttgtcg cacgcatcat gacgtatact ttcttattag ggaaatcacg catattgaaa
      181 gatacatgat agccaacatt tcctaaagta ttataagctt gatttacgtc tggtctataa
      241 atcccatcag ctcttactct agctagttct tttccagtat tgtagtccat aatgaaaata
      301 tactcgtatt tataattagc aatgtgccat ccagctacat gcaagtttgc gttttcgatt
      361 tctccgaact gatcaatgtg ggcgtaattt gttccatctg tcagcgtaga atttgcagca
      421 cctgctcgag ttggatcaat tactggttta tcctccgaag tagttggatt atcatccgta
      481 aaaccatgag ccaaatcata ggctagtttt tctttactta cacccatttc agaaagataa
      541 ccgtaaggat ctgtatgatc gccccaaata ttttgtgtta cccataaatg cgatttgatt
      601 cctggttggt tataaggagt gtctaatgtt aatggaatac catatttcat tgctgaatct
      661 cttgccaatt caacgtatac tttatagttc ttttcaaaag tttctttatc atgtgtatgt
      721 tgtaactcga tttgcacagg acttttggct cacgaggttg tcctactcta catcttgctg
      781 atagatatgc tgaaaggaaa acaccaacat tatgttttgc tttttcagct agaacgagac
      841 aagcaggaat acctttagaa gatatcaaag gttttttagg tcataaagat gtttccacta
      901 cacaagtcta tgcccatatt tctcccgaag tgaaaaaaag atcaatgaat caactagaaa
      961 actatataga agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc
     1021 actaaaacta tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa
     1081 tctagcgaaa aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag
     1141 cctggcttgc ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt
     1201 gatgatgatt ttatccatca attgagattg actgttttgc tctcctatga tttcagctat
     1261 gattgtccga ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa
     1321 ctcgctaagc atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc
     1381 taccattcgt tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg
     1441 taatgtttgg gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt
     1501 aataggttct tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag
     1561 gacatctagt tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac
     1621 tttttcacga ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc
     1681 catcggcgat cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc
     1741 caatggattt cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat
     1801 cttttttcca aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc
     1861 tttcgatacc aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg
     1921 gaaaaaaatg atcgatgatc actagatctg cataccgagg cggcttattt cgagtacata
     1981 catgtataga gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc
     2041 ttgcttcttg gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat
     2101 ctaaataccc tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt
     2161 gtgcaagtgc ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa
     2221 atttttctaa tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa
     2281 ttacgtaacg aagtcattct tggtaaagta cttttgtcag ctttgatttg ctttcgctct
     2341 caaattcgct attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc
     2401 gatagtcttg tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca
     2461 agcgcaaaat tgctagataa tttttttctt ttattggcaa aatctgctcc tctctaaata
     2521 gcaattcagg aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg
     2581 ccggatcctt tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat
     2641 catctgcaaa aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc
     2701 tttctgaacc aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc
     2761 actttctcaa tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata
     2821 gcagattttt cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag
     2881 gttcgctata tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgaa
     2941 tgggacagtc gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt
     3001 tcagttcttc agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg
     3061 aaaataaatt ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac
     3121 ctgatttcta aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc
     3181 cctttctatt tatttaaata cgcaaatctt cctcattatt ttttatttta ctcaatttaa
     3241 tcgtattctc atttcaaaac gaatgtactt taaaaaaaca taataagatc actttgcttg
     3301 taggatgtat tcccttccat tacaatgaaa taaatagcga ttttatgaga aggtgatgtg
     3361 aaaatggaag aaaaaataga agaatccaaa caaaaaatcg atcagttgat ataagaatta
     3421 gaaaaagcgg tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca
     3481 ggtggtcatg tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca
     3541 ctagcaaaag ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct
     3601 agtgatattc ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg
     3661 ggaccgatct ttaccacgat cttgttagca gatgagatca atcgtacgac tcctcgtact
     3721 cagtcagcac ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac
     3781 cctctagaca atcatttttt tgtcttagct acgcaaaatc caatcgagta cgaaggaaca
     3841 tatcctttgc cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct
     3901 tcttttgatg acgaactttt gctacttttg gataaattcg aaaaagtgtt ggataactta
     3961 aatgaagtat tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt
     4021 gtcagtcccg aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagtcatgct
     4081 gccatccagc ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct
     4141 tatgctttaa tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct
     4201 tacgtgtttt cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaagaaa
     4261 caaatcattc atactcttgt tgcacaaatt cctattcctg tgaggtagat tgaggtagac
     4321 aatggaaaaa atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat
     4381 tgtgcttttt gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac
     4441 tctttatgta tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac
     4501 ttgtacattg cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc
     4561 aaaaaagaga actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt
     4621 tgcagaaaaa aacaagagat tttgattctt acaaataaac cgcaagaagt ccaattgctt
     4681 tggcagccaa aagaaagagg atgccatgaa acattaactg tcatttttac agcctatgat
     4741 agtctaggac ttttcagaaa aagtgcaaaa agagagatct cttttcctgt gactgttctg
     4801 cctgcttttt gtaagattca agcagaaaaa ttacagagag ttctggaaaa aaaacagcag
     4861 ctgaacgcgt accaaagagg tcttgatttt cgggaacatc gcgcttatcg tccaggtgat
     4921 tctttcaacc gaatcgattg gaaattgtct gctcatactc aagagtggtt ataccgtgaa
     4981 tacgattatc aagaagaaga atgttctcct cttttttgct tttggggttc gcccagtcca
     5041 aaatttgaac ctctgctaga cttgtatttt tctttatggc atttgagaaa agaaaagcag
     5101 ccggaacttc tgatattagg caaccgcgcc tttcatggca aagaccccgg tcatacctat
     5161 tttgcttctt tggaagctgg cgatgagaaa gcttttttcg cacacttgaa aaaatatgca
     5221 aaaaaacaaa tcgtcctctt tgttcctgag aattctcctg aagtctcaga agctattgaa
     5281 acattgtcaa aagatcgaac ggtttgtctt gtttatttcg tagaaaaaca actcatggtc
     5341 caagaaaaag acaagatatg ttcattgcca ggaggtgaat ggattgacga ttaaacggga
     5401 aaaatggctg cgtggcgttt actcctttgt gatactaagt gtaggtgctc cgatatttgt
     5461 agtagctaat caattgcaaa acagctacct tcttatccta actattggtg tcctatgcct
     5521 tattgtctgc tgtattccta aaaaatgggt acgtttgctg ctttctttgc cagtaatgat
     5581 aggttgtttg tttttatatt ttccttcgaa gacattttcc atattatgga tcttacagtt
     5641 tttttcgtca gtcacagagg aattcttcca tctatcccaa ggtaaacttt attatttgcc
     5701 agaaaagaca gctttttttc tcataatgtt gctgatctat ttatttatta cattgaccgt
     5761 ggattacgac tattggtacg caccctttct ttctttgatg gcttacttca tgatgcttac
     5821 cgtttttcgg aaaaaagatt tactgtacga aatgattgcg gtagtaactg ttttgttcgt
     5881 gtatctggca gcaaggcaat ttttctccat ccggttgttg tcgggaaaca ctcgattcca
     5941 gtcattgact acagttctgc ttatttgttt cttaactttt agcgctatct tgcctttata
     6001 tcttccgaaa gtacaccaat ctctcgagga aacgagtgaa ccgatccgag aatatcttaa
     6061 tgatgaaggg ctttatgaca cactccatga gtatcaatta accggaagtg caagaacagg
     6121 cttcagtgag aatgatgaac agcttggtgg acctttatat gataatggcg aagttgtttt
     6181 tacagcgaac caaaaaggaa atggctattg gaagatcgaa aataaaaatc attacaccgg
     6241 ttctggatgg atagaagatc aggacgcacc catccaacca atccaagaat tccctgttga
     6301 catcacgagc taccaaaccg ttatagaaga caaagaaaac attacattga ccctaaaaaa
     6361 acaagttttc ttaccttatc cttatggaaa tactaccttg actgattttt cattagctag
     6421 tggacaaacg ctttactatt tgcctgaaag ccagcgattc attcctgtcc cgaatgaatc
     6481 gacacgttct gcaactttac aaacagaaag gttagttttt acgagagaag aattaaaaag
     6541 cagtcagtta aaagctgatg atttagaaaa tcagcagaca aatcttcaat tgccaaaaag
     6601 tttgcctaaa cgtattgagg acttggcggt cgaattgacc aaaaatgaag caactttaat
     6661 tcgtaaagtc gaagctgtct aacagtattt aaaaacaagc ggaaatttcc gctattccaa
     6721 aacggatacc gggcatactc caaaagaaca cgattatgtg gattatttcc tctttgattc
     6781 aaaagtcggc tattgtgaca acttttcaac ttctatggtc gtccttcttc gaacacttgg
     6841 tatcccagct cgctggacaa aaggttttac aaatgggctc agaacaggca cagataatca
     6901 agggaatgag gaatacagca ttctcaatag tcatgctcat tcttggccag aggtatactt
     6961 tcctggattt ggctggcttc catttgaacc aaccccttca tttgccaatc caatgggttc
     7021 acaaccagtt gctgaaacaa ccgaaaccgt ccagcctgct atcgaacaac caaatgaaag
     7081 cgaaaatatg gcagaaacta tttccagtga gagatctgag gtcattccat cagaagaaac
     7141 aacactcaca ccagcttata aaatcgtctt attcagcctt ggagtgtgtt tatccgtctt
     7201 gttgttcttt ctattgttat tcagaaaatc tctgcgctgg acaagttatc ttatctatct
     7261 tcattttttt gcttctacta atacgattgc atatcaaaaa cttcttaacc ctttggaaac
     7321 attggtaccg cgcaaacccg cagaatcttt gcaggaatat agcctgcgaa tagaacaaga
     7381 attcaagcta gatctgcgtt tcattcgatt aactactcaa tatgaagcag aaatatatgg
     7441 acgacaagca gatccggtaa aattcgataa aaaaacctat ttagaaataa cgcatataat
     7501 aagaaaaatg gtatacaaaa aaattctgac aaaagggagc cgcttcaaaa aataagataa
     7561 aatttctgga gaatctactt ctggaatgcc gagaaccagc tgattgagta tcgatt
//
LOCUS       DAZUJO010000111         7599 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000111 DAZUJO010000000
VERSION     DAZUJO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.117"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACG8OL_002624"
     CDS             complement(137..427)
                     /locus_tag="ACG8OL_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818551.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACG8OL_002625"
     CDS             complement(545..805)
                     /locus_tag="ACG8OL_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818552.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACG8OL_002626"
     CDS             complement(942..1355)
                     /locus_tag="ACG8OL_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS4818553.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACG8OL_002627"
     CDS             complement(1811..2296)
                     /locus_tag="ACG8OL_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818554.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ACG8OL_002628"
     CDS             complement(2435..2650)
                     /locus_tag="ACG8OL_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS4818555.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ACG8OL_002629"
     CDS             2874..3674
                     /locus_tag="ACG8OL_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS4818556.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ACG8OL_002630"
     CDS             3693..5561
                     /locus_tag="ACG8OL_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS4818557.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ACG8OL_002631"
     CDS             5554..6045
                     /locus_tag="ACG8OL_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS4818558.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /locus_tag="ACG8OL_002632"
     CDS             6033..6587
                     /locus_tag="ACG8OL_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS4818559.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="ACG8OL_002633"
     CDS             6605..>7599
                     /locus_tag="ACG8OL_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS4818560.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DAZUJO010000112         7534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000112 DAZUJO010000000
VERSION     DAZUJO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.118"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG8OL_002634"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG8OL_002634"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS4818561.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACG8OL_002635"
     CDS             complement(2032..3285)
                     /locus_tag="ACG8OL_002635"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS4818562.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACG8OL_002636"
     CDS             complement(3291..4214)
                     /locus_tag="ACG8OL_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS4818563.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACG8OL_002637"
     CDS             complement(4435..6927)
                     /locus_tag="ACG8OL_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS4818564.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACG8OL_002638"
     CDS             complement(6934..7401)
                     /locus_tag="ACG8OL_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS4818565.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2075 a         1638 c         1208 g         2613 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAZUJO010000113         7514 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000113 DAZUJO010000000
VERSION     DAZUJO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.119"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..726)
                     /locus_tag="ACG8OL_002639"
     CDS             complement(64..726)
                     /locus_tag="ACG8OL_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS4818566.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(736..1551)
                     /locus_tag="ACG8OL_002640"
     CDS             complement(736..1551)
                     /locus_tag="ACG8OL_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS4818567.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1813..2256)
                     /locus_tag="ACG8OL_002641"
     CDS             complement(1813..2256)
                     /locus_tag="ACG8OL_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS4818568.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2390..2728)
                     /locus_tag="ACG8OL_002642"
     CDS             complement(2390..2728)
                     /locus_tag="ACG8OL_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS4818569.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2716..3093)
                     /locus_tag="ACG8OL_002643"
     CDS             complement(2716..3093)
                     /locus_tag="ACG8OL_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS4818570.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3317..4510
                     /locus_tag="ACG8OL_002644"
     CDS             3317..4510
                     /locus_tag="ACG8OL_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4818571.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4676..5098)
                     /locus_tag="ACG8OL_002645"
     CDS             complement(4676..5098)
                     /locus_tag="ACG8OL_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818572.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            5469..6143
                     /locus_tag="ACG8OL_002646"
     CDS             5469..6143
                     /locus_tag="ACG8OL_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818573.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(6305..7477)
                     /locus_tag="ACG8OL_002647"
     CDS             complement(6305..7477)
                     /locus_tag="ACG8OL_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS4818574.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         2332 a         1438 c         1292 g         2452 t
ORIGIN      
        1 aaaagaatga cagtttttac ataaaaactg tcattcttaa ttgaaagttt caatttataa
       61 agtctacaga tccatagttc tcatcaattt acgattgttg aagtaaatca aaccaaatac
      121 gatagaagga cctagtagaa tacaccattg gggctttacg aaaaagaaga gaaacaaact
      181 tgtcaatagt aaaaaatcta gtaaactcac ataccatttt ttcaacgtat agtagaacgc
      241 tatccgtaga acttgatttg gtttcgacgc tggatttctc acctgaaaat accagtagtt
      301 gatgctaaag gaaactgata aacaagcaag taaaacaaac aacggaatca accattttcc
      361 ataagaaaaa cgtataaaaa agaaacaatc aaaaatcatt atcacactaa ctatccaagc
      421 aaatagccaa taaaaaaatc cctttagcca aaattggcga aacgtcttta tgaacaaacg
      481 tactggtgaa aaactttttt cttttgctaa ttcttctgtc atacctatca tcacgatgca
      541 acctggacca ataaaaagta aagaaaaaat aaaggaaaag acattccttc catctatcga
      601 caaaaggaac gcagctgcta tatacaagat attgacacac caaaaacaaa ggctacttgc
      661 caatgattga tacaaccaat aaaagaattc catataaaca tttttgtcaa aacgctgctc
      721 tttcataatc acctcctatt ccttcattcc tgatgaagcg atcccttcta caaaatattt
      781 ttgcatcgct aaaaatataa aagcaacagg aagaacagaa acaactgagg cagccatgat
      841 taatgcgtac tctgcatcaa atagcccaat aaacattttc aaccctaact ggattgtttt
      901 attttccgta gaagacaaat aaatgaatgg ccccatataa tcgttccatg tattaacgaa
      961 tgtgatgatc gtcaaactgg caatcgccgg ttttgtaaga ggtaaaatga tcttgcgata
     1021 gattccccac tctgataagc catcgatccg cgcactctca cataaagaat ctgggatact
     1081 actgtagtat tgcttcaaca agaacacacc aaaagcattg aacgcttgta atagcaccaa
     1141 cgaccacaac gtatatgtta aacccacttt tctcatcata atgaactgcg ggatcatata
     1201 agactgccaa gggacagcaa tcgtgccgat atatgctaaa aataaaatat cgcgcccttt
     1261 aaaatttagt ttagaaaacc cgtaagcagc aaaactagaa gtaaacaact gaatcaacgt
     1321 gatcacaacg cttaaaaaag ctgtattctt aaaaaatgtc actaaaggaa tacgagacca
     1381 aataaccaga taattctcta aatgccaagt tttagggatc cattgaatcg gaatcgtgaa
     1441 cacttcatta tttgctttga atgaagcaga aaccatccat aaaaaaggaa tgatcgtcaa
     1501 taaagctaat acaatcagca gaatcatacc caaccattga ataaagggca tgttttttga
     1561 acgaattttt gctttttttc tttcatttcg aacacctcca aagttataat cttcatttct
     1621 ttgtaatgat tacaatccag aacaacggtt attatagctg attcatttcc gttcttctta
     1681 tgtttgtaag cgcttattta acaagttatg taaatctctt tatttttttt gatttaaaaa
     1741 tgaaaaaata caaaagcaag actgtcatac gtctcgcttt tgtattgaaa tttcttcgat
     1801 tagagatact tattactgtt caaaagcttc tttgaaacgg tgaatcaatt cattgactac
     1861 aggtgataaa atccgattct ttttccagac tagcatgcag ttcgtttcaa tcattgggtc
     1921 aagcggaacg aatgtcactc gctttgaaag atgacttaca gcaccttcta tgactaaagc
     1981 agcacctacc tgattctcaa ttagcgcaaa tacattgaaa atcaaattgt acgttcctgt
     2041 gatcagcaac tgctcctttt ttatgcctaa ccagtcagca aacattcgct gtatttctgg
     2101 acgctttgag atcaaaagag gtacattcgc aaagtcttct tttgttatca cgtctttttt
     2161 tgctaaaaca gaattttcta aaatgagcaa tccccatttt tcctttcttg gtaacaaaat
     2221 cgtttcgtat ttctctgtat ctactggatc tagcaaaatc gctaaatcta acaggctttt
     2281 ttctagtcac tccgtgatat catcacttgt tccgctgtgg atatgaaatt gaacgctggg
     2341 ataatcacgg atcaactttt ctagtaatag tgccaatgta ttcgaattat cagcttccac
     2401 acagccgatc gtaaatcggc cgctgaataa ttgttttttt ctgttctcga attcttgagt
     2461 ggtttgattc gttaaatcta aaatttcttc tgctcggctt ttttagaaaa taacctgctt
     2521 ctgtcaagat cattttgcgg ttttcgcgta caaacagttc tgttcctagt tcctcttcta
     2581 actccttcag ttgtcggctt aaggttggtt gtgtaagatg tagaacttct gcggcttgag
     2641 agattgttcc ttcttctgca attgtccaaa aatattttag taagcgtaac tccatagcga
     2701 tgctccttga tttttttact tgatacattc cattttattt ctagataaca cacgatcata
     2761 tttcttgatt tttccttgta aacgttccaa acattcttgc atttcagcaa tttttttcgt
     2821 caaaagttca ctttcttcaa tcaaaagctt acgccgctca aatacagttg cttctccttc
     2881 gctagatagg cgaatatatt caatcaaaga ctcgatcgat aatccggcat ttcgcataca
     2941 gatcgtaaag tcgacccatt ttaggtcttc ttctgtataa tcacggattc cgccttttgt
     3001 ccgattaact ggtggaatca acccgattcg ttcataatag cgcaatgtat cggccgaaat
     3061 gcccttttct tcactgactt gtttgatatt catctgatca ctctttctct taatttcttg
     3121 ccactttact tatgtatttt atttattcta caactttaag tgtactccaa gtcaatactc
     3181 atttctatta aaactaagct ggattatcta tatcggttgt ttgtgtatta tttcaaataa
     3241 aaaagtcttg tttttttatt cttttttgga tatactttta acaatttagg gaaattttag
     3301 gaaaaggggt ataaaaatga caacgtatca gcgaaagtta ttattatcga catcggcggg
     3361 aattgcttta gagaatatgg atattatgtt tttagctttt tcattatctt caatgattac
     3421 ttcttttcat attagtggta cgcaagcggg tttcatttca acgatcacta atttggggat
     3481 gctcattgga ggtatttttt ttggcatcat agctgataaa tatggacgtg tgaaagtatt
     3541 ttctcaaacg gtccttctgt tttcgattgc ttctttattg atgtattttg cttcaaacat
     3601 ctacttggtt tatctctttc gctttatcgc aggtgtagga gcgggaggag aatacggggc
     3661 gtgtatgtcc ttgatttccg aaagtttttc aaaaaaacaa attggacgag cttcttcggt
     3721 tgctggtatt ggtgcgcagg tcggtgcggc tttggctgcg attttagctg ctgtgatcat
     3781 tccaacgttt ggttggaaga tgttgtatgt cgtaggtgta ctgccggtgt tgatggtctt
     3841 gttcattcgt cgcggcttga ctgaaccaaa ggaattcaaa tctgtacaat ctgccgaaaa
     3901 gaaaaccaaa cttagcagac tgtttgaatc aaaacatctt gcttggcaaa catgtggcct
     3961 tagtatcatg gtaactgtcc aaattgccgg ctattttggg ttaatgaatt ggctgccttc
     4021 aattatgcaa aatcgattag ggctttcggt ttctgggtct tcattgtgga tggtcagtac
     4081 tattttaggc atgtctttgg ggatgatgac ttttggcata atcatggata aattggggcc
     4141 taaactagcc ttttctttgt ttttaatttg ttcatcctta tctgtctatc tacttgtgtt
     4201 agcccatagc cagtggagtt tggtagcagc cgctgtgata gtcggctact ttatcaatgg
     4261 catgtatggt ggatatggcg cagtgattag tagtttgtat ccaacagaaa tccgcgcgac
     4321 ggcgaataat ttcattatga accttggacg agctgttggt ggtttttctt ctatcgtcat
     4381 cggtttcttg atggatcatt atagtttaac tgccgtaatc ttgtttttga gcggtattta
     4441 cctagtcagt ctgctggtct tgacaactct tactggtgtt acagcattaa gggaaagctt
     4501 gcagcattga tagttgagtc tgtaaatgac aagaaagagg tgtggcaaaa gcttgcatct
     4561 gcggtaataa gaccagccaa atcaaaaaca tgagacactg tttccgtttg gctggtcgta
     4621 ttacttgatg cagggcagct ttttcaactg ctaatttcct tctatctggc ggctactatt
     4681 ctttgacgtc cgtcatttca aagtacttta ctaataactt agcataacga aaaactgtgg
     4741 atacactcgt atattgagcg tctgcataat cttccaaatt tttctcagga tgttgataca
     4801 tgaattcaat caaatgataa ttatatgatt ttctgtagaa atgaagataa ataatgtaga
     4861 ttgggaagtt ctcctgcttt tccatagaaa agcgccgatt attctcattc ttcaaaatga
     4921 ccttatcagg atatttttgg aacaattctt ctaaatctgt tagataattc aagatagact
     4981 tacttgatac cttcaattcg ttttgtagtt cttctattga acaccaacgt tctgtttcaa
     5041 gcagtaaatg aatcatttta attttctttt gagaaacttt tgttaaaaaa gtctgcatat
     5101 tttctcctcc tgtatctact ttactatact acatacaaga aaaaaaacgg atacaaatat
     5161 catattgtca caccgtcaat agtgcataaa cctgttatta tttctctttt tttcctaagt
     5221 attctattga tatgaatcat ttaatcgttt attatataaa tgtcaataat ctaataataa
     5281 aataacgtac atattttatt attgcatact taataacaga actatagcaa aaaatcccgt
     5341 gtatcaaaac gggattagat taagtttcct caatcactat tcataacaca attattataa
     5401 caagtaagta aacaattgaa aaggacattt attgaaaacg gttgtttttt acttaaggag
     5461 gaaatttagt gaaaaaatta gttgcattgt ttttggtcgc aggaagtttg ttgagtgggt
     5521 gtggtgctac tacgtctact actcaaagca caagttcagc tcaggcttcc agcagcagta
     5581 ccacgatctc cgaaagtacg gcagattcat cgaaagaaca agaacaaatt gaaaagaaaa
     5641 aagaagaaga gaaacaaaaa gcggaaaaag ctgcactaga aaaaaaagtg acagaagcgg
     5701 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5761 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5821 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5881 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcgc
     5941 gacagcaagc agcagctgaa caggcctctc agcaggcaca agccgcagct cctgagcaaa
     6001 ataatgaaca aactgtctat gtcacaccga ccggttcaaa ataccataca cataaatgtg
     6061 gaaatggtac gtatacccct gccactttaa gcgaagctca agctcgtggt ttaacccctt
     6121 gctccaaatg ttatggaaac taattcaaaa gttatactat aataataaaa ttcttctgaa
     6181 acataaaaaa atagaagctg gcgttccaga tagtaaacca aaaatagcag tttacacgtc
     6241 catttcgtca gcttctctct tttttcattt agtctgttcc ctctgctttt ttggacatct
     6301 tcattcattc tttctcaatc aaatagtaca cctctttttc ttttcgcagt ttatatcttc
     6361 cttttttcaa tttcattgaa aactgtttcg aatgtttatg ttttacatgg attttaagca
     6421 ggagctcctc atgttgcaag cgatagttcc tgccttttaa atcagaaaat ctcactagtc
     6481 cgtattcatc agaagctttc acaaatggaa ctttttctct agtaatatag tgtttgccgt
     6541 tgagcgtttt tacagaaaag accattcctt cgattggtcg tccgtttgtg ttctttacat
     6601 aaaaagataa aggatagttt cgcttagcga tggccaaaga atctaaataa gcgatgagta
     6661 gccaaactaa gccgattgat ccaagtagta taccgataaa ccaaataaaa aatttccatt
     6721 ttttccattg atcaattcct tggatcgctt tttttgcctc ttttgcttga taaggaatac
     6781 gatgccccgt cacaagcagt cgatgtgtat tgaccatata aggagtacag gtaagcaaag
     6841 tcacgagatc tctgcctttt tggataccca aagaatctac ttcatcaggt aagaccactt
     6901 ggattttttc tacttgatac gccaatgtct ttccattgat ctggatgaaa aactggtctc
     6961 cttttttcaa cttaggcaaa tctgtaaata attttgcttc tgggagtcca cgatgcccag
     7021 aaagaacggc atgggtgttt ttcccgccag tcggataaga actttcttcc aataatgacg
     7081 ttcctttttg caaaaatatt tccgttgtct gatcgaatat aggtaagctg acattgattt
     7141 ttgggatttc gatgactcca agcatatgtt ccataaaaaa ggcattacta ggtaaatctt
     7201 ttaatgcttt tgcatcaact gcttggttga aactgcctat ccctggtgat acgttttgct
     7261 ctgccaactg ttggtttttc tttgtcattt tttcctgctg tttttttatt tcctctgaat
     7321 tcttattcga tgcttgcttt tgataatgga tgatcatttg ctgggcaaga aaatcattca
     7381 gtgaatcttc tacaaaagga taagaaaaga tcccgatccc aacgactaaa atgattgcca
     7441 tgaagatgtc cagtattctt attctgcttt tattcatcat gtccacctat tttctaatat
     7501 gtatatgaag aggt
//
LOCUS       DAZUJO010000114         7345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000114 DAZUJO010000000
VERSION     DAZUJO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.120"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..885)
                     /locus_tag="ACG8OL_002648"
     CDS             complement(<1..885)
                     /locus_tag="ACG8OL_002648"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4818575.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTS"
     gene            complement(1262..1900)
                     /locus_tag="ACG8OL_002649"
     CDS             complement(1262..1900)
                     /locus_tag="ACG8OL_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818576.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1904..3886)
                     /locus_tag="ACG8OL_002650"
                     /pseudo
     CDS             complement(1904..3886)
                     /locus_tag="ACG8OL_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4066..4731
                     /locus_tag="ACG8OL_002651"
     CDS             4066..4731
                     /locus_tag="ACG8OL_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818577.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5128..7161)
                     /gene="brxL"
                     /locus_tag="ACG8OL_002652"
     CDS             complement(5128..7161)
                     /gene="brxL"
                     /locus_tag="ACG8OL_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFS4818578.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         2359 a         1428 c         1089 g         2469 t
ORIGIN      
        1 tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta cattagcgtc
       61 tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt ttgtgtctgt
      121 tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt tttggttttt
      181 caccatttta accacataat attccgtagt ataagttgaa gcattggtag aagtgatcac
      241 atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt
      301 tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag tatcagttga
      361 cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct tcacatcttt
      421 tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat ggaaagattc
      481 ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac cttcttgaaa
      541 ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa
      601 ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattggg aatcgatttc
      661 tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga tcaactgttg
      721 cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt gttcgctgat
      781 tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc ctgacaataa
      841 gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc aattctccta
      901 cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat catcaagaac
      961 acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa tccagaaagt
     1021 tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac ttcttttgtt
     1081 accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt agaacaaatg
     1141 ttatcgattt tgtttttctt cttccgctgt tgggatattc aaatcagaca cttttatgtt
     1201 cgattttcca gacatttttt tgttgttttt attagaaaaa gtttgttttt tcatttgttt
     1261 tctaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc gtgaccacga
     1321 taactaacgt accttgggaa gcctgttgcg ataagatttc aacgacttgc tccttatttc
     1381 gtttatctaa tgatccagtt ggttcatcgg ctaaaatgat tttgctattc ttcaataaga
     1441 ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata ggcgtactta
     1501 gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc ttatctgaaa
     1561 catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt tcatattcaa
     1621 tcaacccaaa atcttgaaaa agaaaagaga gggcatttct ttttatcaag cgcttctctc
     1681 ttccagttaa atgttttgtt tcttgattaa ataaataaaa ttgccctgaa ctgaattgat
     1741 cattcaatcc taatatattc aataaagtac ttttccctgc tccgctctct ccaactacag
     1801 tcacaaaatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt ttgtttcgaa
     1861 tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct ccattcatca
     1921 gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga ataaaatcaa
     1981 tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata agaaaaagga
     2041 agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct ttatacgttt
     2101 caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg tagacaaaat
     2161 atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt aaaaatatct
     2221 gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca gtctgagtat
     2281 acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc tcaaataacc
     2341 tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct ccgctgagaa
     2401 tatttctttc ccaatacgta ctgtttttca atgtcagtat ttcaacaacc ggataatgtt
     2461 cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt ataaatagaa
     2521 atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc aggtcatttt
     2581 ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt tctttttcgg
     2641 ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga taattcagat
     2701 ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct gatacattga
     2761 ctaatatact tccgttttta ttcaaagttt catataattc ctgatcggct gctgatgcaa
     2821 aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga taaaatacat
     2881 gatagtcctc accagcgatt tcttttggtg tttcatggga aacagctaac tgtgaagaag
     2941 aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa aacaacaatc
     3001 caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa gcgatttttt
     3061 caactaataa aagagaaatc aaagagatac cctatattcc gctgaacaag aaaacgtaca
     3121 tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca ccaatgattc
     3181 ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac aatacttgat
     3241 gtaatgaaaa gccattattc aacaataaac tgatttcttt attcatcaat aagaaaaagg
     3301 atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga taattatata
     3361 cgtctgtact tgaaataacc gaatacgaat agctattttc aaagtcttca aaaatattag
     3421 tggcataatc agtgtgaaat gcttgattga atcgttccac aaatagtaaa atgaaccgtt
     3481 cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct tttattttga
     3541 tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc ttctcgtgag
     3601 aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt atcttgaagt
     3661 tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa gaaagctcag
     3721 tttctgatga gacattttca aataccatga ttagctgctc atattcttct tgcccaataa
     3781 aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat tcatttgtcc
     3841 gatagagtac ctgtttattt ataataacca aggcaaaacc actcatgata ccggtcacta
     3901 tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct tacttaaatg
     3961 attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa atgtataggt
     4021 catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa catcggagaa
     4081 aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg cgaaaaaatc
     4141 ggtgtttccg ataaaacaat ctctagttgg gaaaattctc gtacaatgcc agaccttgaa
     4201 atgctgtcac ttttacatca gactttaggt ctgccgattg attttccttc tattgaatca
     4261 gctaccgaat caatagaacc aacattgccg gatgaacctc ttgttatcaa gaaatcttat
     4321 tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct cgtcttttgt
     4381 ttggtctatc tttttcttat caattatcaa tataaaaatg aaacggttga tcgattcaat
     4441 ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt ggaaaattcc
     4501 atcgaacatt gggtatgtaa tgatatcttt gttcatggta catggctgtc ttttcacgct
     4561 ggagaggtag aaaaaggtaa gaatcatgct gtaatcaaac acaaaggaaa acacgtcatg
     4621 aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat cctcatccaa
     4681 gactatgacg atgccttaaa aagaagccct caggagccag ataggcatta agtaggagat
     4741 gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc aaaaaaaaac aggggtacaa
     4801 attaattgaa aaagctttga gatgaaagtt cctaaataaa aaagttcaga atgataggta
     4861 aaaaaatgac taactattct agtattaggc tatttgtatt attcttctgt tatttttata
     4921 tcaaaaaatc cagaaataac taaatatagg tatttctggc ttatagtata atatttacgc
     4981 cacagccccc aatgattctt tttggaatca tgatcaagga cggacataca caaaaatatt
     5041 ttgcccattc gatccgaata gattaacgac taatctagta agtaatatag attggtacgt
     5101 agtcaaacca gcaaatggaa aagttgtcta ttccacccca agggctttaa agacagcatc
     5161 ttccggcgtt tgataaaaaa tcaaattaaa ggtacccatt aattctgggg gtacagttcc
     5221 aatatcagca gcgcttatca taggtacaag tacttttttc gctccggcat cttttgctac
     5281 ttgtaaacag ttagctaaat tatctacctt aatcatagaa ccaccaattg taatatcccc
     5341 taaaataact aaacttggta atacaggttt atttaaggaa actgagcata aagctattat
     5401 agttgataac gataatgttt ttgtcatacc aatgccattc atatcttgaa catgaacgac
     5461 aaaatctttt gtcgtcaaac taatagactg actaacactt ccataattag ctcttaaata
     5521 tcggtatgcc gaatccatcg cttctcttgc tccagcatct gaattgatcc cagtacgttc
     5581 aaactttcca tttcccgaag tcatttgcgt ttccaattta taggtaccta attgcccagt
     5641 tttgccataa gataccgtgt acaaatgccc tggcttccct aacccttctg gaatcaaagt
     5701 tcctccacct tgttcaggaa cagaaacata ctcttctcgc tcttcttcta aatcaatgta
     5761 tgaaaaattg acatcataaa attccattcc accaattttc tttaattgtt cttttacacg
     5821 acgacgcatt tcaagagcaa atcttagtat ttcttcgaca tcactttttt caaatctacc
     5881 atcaggatac aacagtttca tcatcccaga tacaatccga cgaactgcaa tcgtatcccg
     5941 ctgatttaat tgagaaccaa gtttgaaata tttatcaaaa ctatcgccaa aactaacttt
     6001 tcgaagctca cgcatgactt cagaaaaata atctgtaatg aagccatagt catttgtaaa
     6061 aaaatctggt cgatatttag gaatttccca acctggtaaa taacaatgca tccgatccaa
     6121 aaaggctgaa tccgtcccca taacctccgg aaatggctca aataaattcg aagttttcaa
     6181 tattacatca acgctttgat taatatttcc tacaaaagcc attgatgctg aagcattctt
     6241 ttcttctttg cctctagcaa aagatcctga agccatgtaa tctttcatga tttgaacgcc
     6301 atcattatcc ttaaatttaa tcccagcaac ttcgtcaaaa gcaacacagt cccacatacc
     6361 tacaagtccc attgtacgac tagccatatt atagaaaagg tttgctactg ttgtttgtcc
     6421 acctgaaaca aggattgagt ttggtgaaat ttctttatat acatgcgatt tacctgtacc
     6481 acgtggtcct agttcacaga gattaaaatt attttcaatt aatggaataa gtcttgctaa
     6541 atgaagccat tttacccttt tattaaattg tgtaggttcc ataccaatgg accgtagaag
     6601 taagtcaatc cattcatttg tagtaaactc cttgcgagcc atcttgtaag cctcaatgtc
     6661 taaaccaggc atttggattg gatcaacctt ttttgcaata aaagggctat tccaacgatc
     6721 attctcatca taccaatatt ccatcgaaac aatacaccag atccccccac ctaataatcg
     6781 atcaaatttt ttcggatact cttcactaat ttgaacattt tttaatccca ggttagagaa
     6841 ttctgcttca taacaatctt tcttttcatt taaatgtaca gttactttat caataactgt
     6901 ataaccaccc aattcacgaa tttttgcttt tactttttct gcttcatcag gacgcacata
     6961 gtttcgagca agaatatttt taacattttc cacacctttt tcaaccaatg tttcatccaa
     7021 tgacgaagca tacataccaa gtaaatattc caatacataa acaggtacat tcgcaccttc
     7081 tttaattttc tttgttaaat cttttctgac aatctttccg ggaaagaatt cagataactt
     7141 taaaatttcc tgttgttcca ctagaattcc tccatgtgtt cagtcattat aatagcaatt
     7201 aaagtatatc atacttaacc agtaggcttg ataatacaaa agaaaaagta tccacaaaaa
     7261 attgtggata cctcgttttt atatatcaaa atcaaatgat tctgcaaaag gattatctat
     7321 aatgaatttt attcgctcgg tcaaa
//
LOCUS       DAZUJO010000115         7153 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000115 DAZUJO010000000
VERSION     DAZUJO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.121"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1368)
                     /locus_tag="ACG8OL_002653"
                     /pseudo
     CDS             complement(1..1368)
                     /locus_tag="ACG8OL_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1611..2066
                     /gene="tnpA"
                     /locus_tag="ACG8OL_002654"
     CDS             1611..2066
                     /gene="tnpA"
                     /locus_tag="ACG8OL_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS4818579.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2331..2429
                     /locus_tag="ACG8OL_002655"
                     /pseudo
     CDS             2331..2429
                     /locus_tag="ACG8OL_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2652..2882)
                     /locus_tag="ACG8OL_002656"
     CDS             complement(2652..2882)
                     /locus_tag="ACG8OL_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS4818580.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3112..3930)
                     /locus_tag="ACG8OL_002657"
     CDS             complement(3112..3930)
                     /locus_tag="ACG8OL_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS4818581.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4091..4780
                     /locus_tag="ACG8OL_002658"
     CDS             4091..4780
                     /locus_tag="ACG8OL_002658"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS4818582.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4794..6296
                     /locus_tag="ACG8OL_002659"
     CDS             4794..6296
                     /locus_tag="ACG8OL_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4818583.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6309..6785
                     /locus_tag="ACG8OL_002660"
     CDS             6309..6785
                     /locus_tag="ACG8OL_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4818584.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6882..6992)
                     /locus_tag="ACG8OL_002661"
                     /pseudo
     CDS             complement(6882..6992)
                     /locus_tag="ACG8OL_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2316 a         1131 c         1295 g         2411 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggatccg atttttggct actgctccga cttgtcctcg ataagaattg tagcgccgtg
      541 atttacgtgt gtatgtcgtt acttttattc ctagcttctt gatcaggcgc tgaactttct
      601 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
      661 ccttgtgctt ctcccgaact ttaagcattt ccgcttcaat ctcttgatct ggatttttac
      721 ggtccaatcg tttttgccag tacataaaga ttgccttagg gaactttaaa gtttctagaa
      781 gctcaactaa tttaaacggt cctcggaggc ttgcgatgat tcgtgcgatt gcttcattcg
      841 tcgttgttgg gcttcctgtt ttcgcagctt cctcaattct tttaaaaagg cattttcaat
      901 ttgaagggaa cggactcgtt tctccaactc ctcgatgcgt tcacgttctt tagacgttgc
      961 tttagttgta gattttttct tatttttggg ttcatttttc ttagacaaga taggtggaca
     1021 tcctttcgtt tttgagagtc catcgatccc accctcacgg aattgacgca tccaactagc
     1081 gatcaaatta gggttgttca tgttaagtgt gttggcaact tcacgatagg acagctcact
     1141 tgttagatat aactctatcg catccagctt aaattgaaca gaatattttt tcttttgtcg
     1201 ctttcttact agaccctctt caccaaattc ttgataggca ttgatccagc gcttcacttg
     1261 tgatggagcc ttaatggaat atttctttgc cagataatca cttccgcctt taccatccaa
     1321 gtaatcatga acgatcttta atttaagttc aaaagtatac ttagccataa aaaataccga
     1381 cctccaaaag ttagattttt tggtctaact tttgggggtc ggtacaaaac ttaaaagttt
     1441 tagacccttt tattttctta atgacttcag tcagttttgt gcgtaaagcg tgcgaaacaa
     1501 gaaaaccgcg cgtttctacg cgtggaagaa gtatctttag cgataaaaac tcctttcgat
     1561 atactagtga gtggctacca accgctcatc agatcgaaag gagttttttc atgtcaaatg
     1621 acgataaaag tttagcatat actcgttgga attgtaagta tcatattatt tttacaccaa
     1681 aatatcaacg aaaagtaatc tatgggcaat tacgaaaaga tcttggaaaa atacttagga
     1741 agttgtgcga aatgaaagat gtggaaatca tagaagcaca tgcaatgcta gatcatattc
     1801 acatgcttgt tcgagtacca ccaaaaatat gtatatccag ttttatgggg tatctgaaag
     1861 gtcgtagtgc ggtactaatc cacgagcagc acgcaaatct caaatataaa tacggaaaca
     1921 aatccttttg gtcgaagaga tactatgtga gtacagtcgg attgaatcaa aagacaaatg
     1981 aaaaatacat ccgtgaacaa gaagctgatg atcgcattcg atatattata agtaaaagag
     2041 agtacgtaga accatttaaa aaataaagag aagtaagtga gtgaagatga gcagttggcg
     2101 gtcttttaaa aatccccttt taaggggcag cgagtgtcga ccccttctag ggggattaaa
     2161 aatacacccg tcctcacggg tgtatgggtt actgatagac ttgattttaa ctggtgtcag
     2221 aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt acggctattc aagaattagc
     2281 acaagcaacc aagattacaa aacacaaatt caaacattag aaaatgctgg cgcagaaata
     2341 atcttcctag aaaatcactg gattatcgaa cacctttgga agtatttttg agttacgtaa
     2401 gtattgatga tctgtctaac ttaatttgac aattaagagt atatttactt cagatggtaa
     2461 ggcaacaaaa taaaatacaa taggcagatg aactaataaa taaaatctgt attaaataca
     2521 gataaattaa tataaaaatc aaaaggatca taaactttta acaagtgatg aaatgggtgg
     2581 aaattgattc ttatagacag tttgtctcat aacattgaag tattttgcaa ttttagcatt
     2641 gaaacacttt tttaatcttc aacgttgttc ttataaacaa gatgattggg aatcttttga
     2701 attaacactg tatagcttag aacacccttt atagaaaaca ttcgcttagc aatcttaata
     2761 attccttgtg cccgatgtcg tttagattca gtaggttcct gctcatcgat catggaaaaa
     2821 atttgaagga tcagatcctt aataaattta tttaagagag gatgatccat tgcttcatcc
     2881 atactcgata aactagtgat aatgagctac acgtttttct ttcaagttga tcaatagctt
     2941 ccacgataaa aatatcttta gataaaatca agaaccgtag gaactacagg gatatttggt
     3001 caataagagc caccgctatt agtaaaaaat aagctatcaa gtatgctctc atcccaagaa
     3061 ttgcctactt caatcgttaa aaaccgttag gtttagctaa gtgggggtag ttcactcaat
     3121 acttttatca ataatagagc gcaaaacttt ttctcttaat tcaactgaat caatattatt
     3181 gttttgttca taaccatgaa ctaatagatc acaaatataa agttgtgata tttttccagc
     3241 taaagatcca ccattaagaa attcttcaat ggctgtttgt aaaactaagt ctgcagactt
     3301 tcctataggt gaatgaatat agttagttat agcaataatt tttgcgccat tttttttagc
     3361 tattttcaat gaatcataag tatctttcgt tttcccagat aaagaaaaaa tgattactaa
     3421 atcattaact gttaataaag aagcgacttg ggcttgaaaa tgagggtcta gtacagcttt
     3481 tgcttgaact cctacgcgaa gaaacatatt ttctaaatca agactagtat ttccactaga
     3541 tcccactcca aatatatata tgttttttga ttgagtgatc attttaatgg cttcattcaa
     3601 tttttcttcg tctaaaagcc taatagtgga atagagagct tctgttaaac tattagcaat
     3661 ttcatcatag tactttacac tagtcggatg ttcttttttt tgactaaagt cttcctttgc
     3721 aatttcaatt tttaaatcag aaaaaccaga aaaaccaatt ttttgacaaa agcgaataat
     3781 agtagcatcg ccaacttttg tgtttttctt aatatcactc atcgtactat aaataatagt
     3841 tttcccagaa tttaaaataa aatcagcgac ttttttttca gacttcgtta aaaatggata
     3901 attttcttta attaaataga tataactcat acaaactcct ctacaatttc aacttttctc
     3961 cattatactc caaaataata aaaaaaactc ctaaatactc catttttatc tttactttta
     4021 tgaaagcgga tgctatattg tttttggagt tgttatccaa aaataataaa aaattggagt
     4081 gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt tgattgtttc
     4141 ctgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa tgccttattt
     4201 tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg ttcgagatat
     4261 tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa gagattatcc
     4321 accagaatct ccattcatta ctgccactat gagagaagta gatgaattag tagaaaccgg
     4381 tgttgaggta atcgctcttg attgcactct tagaaatcga catgatggac tatctataaa
     4441 ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg cagatatttc
     4501 tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag gaactacttt
     4561 aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt tgattaaaaa
     4621 attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc cagaacaagc
     4681 aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta ttactcgacc
     4741 acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat aaaatgttta
     4801 aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta ccagtagccg
     4861 gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat gcttatccat
     4921 ttttagatca accgtggttg catacaattt taagtattat gagttatgct ggaaatgcag
     4981 tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt gcaaatggag
     5041 ataaaggaac agctggttta gctggtggag tagcttatct agtatatact gcaactatta
     5101 gtgggtttct tgaacttttt tcagcaaaag atgcaacact agatacaggt gtagtgggat
     5161 caattgttgt tggtggtact gttgcatttt tacacaatcg ttatagaaaa attgaattac
     5221 cacaattctt aggttttttt ggaggatcaa gatttattcc gattgtttct agtttagctg
     5281 caatcattat tggttctttc ttttatttaa tttggccacc aattcaaaat ggtttagtag
     5341 ttgctggtga acaaattgcc caaatgggta gtttaggaac ttttttatat ggttttttat
     5401 tgcgtttaac aggtgctgtt ggactacacc acaccattta ccctttgttc tggtatacat
     5461 ctttaggtgg taccgaaact gtagctggat caacaattgc tggagcccaa aatattttct
     5521 ttgcccaact agctgatcca aaccacactg gtctgtttac ttatggtact agattttttg
     5581 caggaagatt tgcaacaatg atgtttggat taccagcagc atgttatgca atgtatcgtg
     5641 caattcctaa gaaaaatcgt aaaaagaatg gtgggcttta ttttagcggg gcccttacat
     5701 catttttaac tggaattact gaaccagttg aatatatgtt tttatttgtt gctccttggc
     5761 tttatgtgat tcatgcattc ttagatggat tatcttttta ttttgctgat atcttaaata
     5821 ttcgtgtagg gaattccttt tcaggtggtt tgatcgactt tttattattc ggtgtacttc
     5881 aagggaatga caaaactaat tggttaaaat taattccttt tggtattgca tgggcattta
     5941 tatatttctt tgtttttaca ttctttatca aaaaatttaa ggtagctatt cctggaatgg
     6001 aagaagaatc agaagttcat tctgaaaaag taataaacaa aaatacatca ttacatgaag
     6061 agtctcttat cattattgaa gctcttggtg gaaaagaaaa tattgagtct gttgaagctt
     6121 gtgcaactcg tttgcgtgtt gctgttacga atggggagat tattcaaaaa caaacaataa
     6181 aagacactgg tgcaactgct gtcttcgaag tgaagggtgg catccaagct gtttttggag
     6241 ggaaagctga cttattaagt caagaaatca atcaaatatt aggaaaagat aattaggagg
     6301 tttttaatat gtttgaaatt ttcaaaaaga aaaaacaaga aactttttat gcaccttgtt
     6361 ccggaaaaat catttccatc acagaagtaa atgatcctgt cttttcacaa aaattactag
     6421 gtgatggttt tgccgtcata cctgatggtt caaaaattta tgctccattg gctggtcgag
     6481 taacaaatat atttcctact aaacatgcta ttaatataaa aagcaactca ggcattgatt
     6541 atttgattca tattggaatt gatacagttg aattacaagg agagcctttt tcgatttgtg
     6601 ttcaagaaaa tgatgaggtt actcctgaaa cctcgttggt tgatgtggat tttgacaaaa
     6661 taataaaatc tggtaaagat acttctgtta tagttgtttt tccggagaaa aatcagttca
     6721 atgatttaat aatagataat ggtcgaattg ctcatgggaa ttcatgtgga aaaatcactt
     6781 tttaaaaaag tgtatataaa aagctataaa ataagctaaa taggatattt gtcaaatgaa
     6841 gttggtgaac agaaagaact gttttctttc tgttcatttt tttattttat tagtaatgat
     6901 tccccatgcc aaatcatgat ccgtaattta aagttataaa agcttcgaaa tccattggca
     6961 tttcgttgga ataccttgat atgattattc aaatataaaa tatagagaga agagagagag
     7021 ggaagatatt tataaagggc aaagttatgt ttactcaact taggaaatag aaatagatca
     7081 atttatattc tcttgtcaat cgaatatgaa ccttagcact atataccaaa tatagtaaaa
     7141 tgctgaaaga tgt
//
LOCUS       DAZUJO010000116         7126 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000116 DAZUJO010000000
VERSION     DAZUJO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.122"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..435)
                     /locus_tag="ACG8OL_002662"
     CDS             complement(94..435)
                     /locus_tag="ACG8OL_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS4818585.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFKMTLTNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(1054..1386)
                     /gene="traE"
                     /locus_tag="ACG8OL_002663"
     CDS             complement(1054..1386)
                     /gene="traE"
                     /locus_tag="ACG8OL_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS4818586.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2233..3273)
                     /locus_tag="ACG8OL_002664"
     CDS             complement(2233..3273)
                     /locus_tag="ACG8OL_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS4818587.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            4439..4525
                     /locus_tag="ACG8OL_002665"
                     /pseudo
     CDS             4439..4525
                     /locus_tag="ACG8OL_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            5079..5303
                     /locus_tag="ACG8OL_002666"
                     /pseudo
     CDS             5079..5303
                     /locus_tag="ACG8OL_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5563..5817
                     /locus_tag="ACG8OL_002667"
     CDS             5563..5817
                     /locus_tag="ACG8OL_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4818588.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            5818..6135
                     /locus_tag="ACG8OL_002668"
     CDS             5818..6135
                     /locus_tag="ACG8OL_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS4818589.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            6694..7126
                     /locus_tag="ACG8OL_002669"
                     /pseudo
     CDS             6694..7126
                     /locus_tag="ACG8OL_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2409 a         1144 c         1063 g         2510 t
ORIGIN      
        1 attccttact caatgcctaa ggtaatacta cctttttatt aatcgtcgtg actccttcta
       61 agcctttatc tgtaaaattt attcctccag tttttattct tctttatccg ctacaaattc
      121 aatgatttca acgctgacaa tataatcact caaatatgac caagtatctt caacagataa
      181 ttcgtctcca ttatcattcg taagcgtcat tttaaaccat tcattacctt catcatcttc
      241 aggtagcaag ccgaatccaa ttggattatc agtcaatttt tgatagtata aatcatcctc
      301 gtctaatgct aaaacatcta atcctttaca atttccgcct cttctaaacg tcacaaatcc
      361 tttgtaatcc caacgttgga aagaaacctt aatagtatgt gtgcccaatc gtatattcgg
      421 atcataattt ttcatttgtc attcctccat tacgtaaatt tcattttctc tatgttttat
      481 atcaattttt tcactttatt caggactcgt tcatatttta tcgtttttcg tttacttaaa
      541 aatacataaa aaatatcgac tcgtttatgg cgtccgaaac gagaaaccct agtagaaatc
      601 ggtaaatgct cgattgatcc aatgaccaac gtcctcgcaa gtatctacat gtactgcagc
      661 acacgacaca atttcttgtg cgtcctcccc attacgagaa gacctactcc ttattccatg
      721 agtttgcgtc aaagcctaga tcccttagta gctgctcatg attaatctag gcgctattct
      781 aacttgtcta acttctaaat tccctcttgt ttactctgtg catattattt acacaaattc
      841 atttcaccac tatccaaaca agggcattta ctcgattgac caatttttta cgttaccaat
      901 cgtaaaaacc taccccgtca ccccccgaaa ggaattgttg atagatcaag tctactcctt
      961 atttttctag taattctaag ttcttttaga agtctcgaca atattttcaa gcaagcgaaa
     1021 aacgataaaa tacaagttat gagaatgatc aattcagctg attaaacgcc ccatcccttt
     1081 tgcggtacaa cgcaagactc cattttttct tcgaccaatc agtacaccgt tttggtcata
     1141 aacatgtttg attgttggat atagttcttt taaagggata ggatctttta aaattatttg
     1201 cttttgttta ccagcgtaat aaatcatttc ctctgagact ttgtctttct ggattatata
     1261 tttcttacac tgcacaacgg ttttactttg gccagttgat agtttcttcg tgattttctc
     1321 tgtagcggta caccgtttac cgtagtgaca aatatcttcg agactcaaaa ttcctgtata
     1381 tgacataaaa taatccaccc acttacttta aatattttta ataagttttt cttgttattt
     1441 ttcaaaaata gagaattgat tttcgttcat gacaaacaaa aaagagggaa cgcatccaaa
     1501 catgcgttcc ctctaatttc agcgttcgtc atacggcaaa gagccacacg gacattttta
     1561 taacattatg cttaggcata gattcatcgt aatatcacgt atttttaacc ttaacacagc
     1621 caaaaaaagt ggtatcactt ttgaatatct gttatactaa atccgttgcg ggtaataccg
     1681 taacactgaa aggtaaacgc attaagggat taaaggttgt cttaatgcca cctgtggacg
     1741 tcgacgaagg ttaccagcct tccgagtctc acagggcctt tctttttttt gtattttcaa
     1801 ttgtccaaat ttatcgtcca aaagtgcaga acgaaaatta attcatcaca agcgtagcag
     1861 acaattaaat ttaaaacaac taaatttata caaaaaaatg taagcgaata atattcgtat
     1921 tgcctgaaaa gaagtaatat caaggttttt agctttttta aaaaatgaaa ttacctctct
     1981 tcgcgtatta ttataactaa aaaacagcaa catgcaaacg tttgttctct tttttgtgtt
     2041 ggaaattaat tattaactat taacacttat gacgtaaaaa gctacgtttg tgatttttat
     2101 cataatattt taactcataa attctattaa aatagctatt tctttgtaac atttgtaata
     2161 taattagaat aataaaaaat ttttaatttt aatgctactc catgaagcaa caatcaattt
     2221 gtcagtgaaa tttcactcta accagtcttt cgtaatagtt tttgggaagc tttcagaatt
     2281 caaacgtttt tcacttgata ctttagcagc tattggtttt tcgataaacc aattttttac
     2341 atagacaaaa atcaggttct ggatattttc aactttttcg gtttttaatt tttgataggc
     2401 ttttaacagt gtcgtgtata attcttgttc tgcattgaca taataactat cgagttcttc
     2461 aaaaattatt gaaataccac tttctgtttc ggcttttttc tttgcattat gaatagtctg
     2521 gacagttttt tgagcttcac tgtaacttga agaaaatgtt tttattaaat ttaatactcg
     2581 ttcagggata aacgtagcaa tgctgtcgtt tgtcataatt gtctcaaaat tgtctaacaa
     2641 caattgatct tgtaggcggt ctttttcagt gtctatattg tgtctattag tgtctaatat
     2701 attattatta agttctggta tgattttcat accctctgat ttatcaacgt tttggcacgg
     2761 tatgattttc ataccctctt gagccccaag ggattcagcg tctgattttt cgtcgagggt
     2821 gggtatgatt ttcataccct ctgatttatc aacgttttcc ctattctctc tatcaaattt
     2881 ttcgagtaag tagatatcat tttctgagac ttttaattct gctagatata gtctattagg
     2941 attatttttc cctaactgtt tgttaaatcc catctgcttt tgtaataata aaccaaagga
     3001 ttccaactct tttttgattg ctatcgcttt ttgtttttgg cagtttaata cgctacatag
     3061 ttcatttgtc gtataagtaa aataaacatt accttcttca tcgatttgcc gtttgctgat
     3121 tgcgatctct aatcttgctt ttaaaagcat ataggcaatc ttagttgaat ctttcatttt
     3181 tttgtattcg tcagaaacaa taaagacttt tggaaattga taaaataatt cgttgtaaac
     3241 tctatccgcc ttaaaaaaat taaaatcagt cattgtagat cctctacttt cacgttgttt
     3301 tatttatgta aaattaccta ctcttttcga aaatagcaaa gataaataaa aaatgcaaac
     3361 gcattaaaac tgttttttaa ttaaatacta cctaaaatca gaaataaaat gaattatgta
     3421 ctacctttaa agtcattaag aactacttta aataaatatt atttataatt aaatactacc
     3481 agaagaacgt atgaataaaa tttagtttta aaattaaaga taattaagta ctaccagtgt
     3541 caaaacaaaa aataaattaa gaactacctt atgcatataa aacatactac cattactagt
     3601 aatatatact accttatttc agtagtatat attatattct tcttataaga caaggcttta
     3661 ggctagaaaa caatagatta tatactacta aaaaatacaa taagtactac caatgtcaga
     3721 tatatataat aattatgtac taccattaaa attaacttat ttaataagat actactgtgt
     3781 taatctgaaa aaaattactt taaagtagtt acatactacc tgttaaatta gatactacca
     3841 ttaatactta aattagatac tacctatatt tatattaagt actaccgttc taggaataag
     3901 ataataaaaa aaaactacca aattgcaatt aaatactact taaaatgtaa aaaataagaa
     3961 ctacccaaat taggtagttc ttaataagag aatatcagat aaatttgtac aagtcataaa
     4021 aaaattaatc ttaaattttt aaatatatat acctgttaat tataaactac tttatttagg
     4081 tagtacataa ttaacagata aaactacatg cttgtagttt gtctagctaa tgagttgcat
     4141 atgatttata taaaggtgtg cagcataact tagttgaagt taagagaata gaataaatat
     4201 tttttgtttt gctaataatt aaacactacc taaatatggt agtgtttaat tatataatat
     4261 tcattttgtt acaaataatc gtattataaa agcataacaa ttgggataac tattttttag
     4321 attatttaag tactacttaa attaggtagt acttaaattt tggtttaagg tagtaggtaa
     4381 ttaagaaata atatatatct tcttttataa tagaagggga gaggcctagt taaaagcagc
     4441 gacaattaat aacaatgtat ttaatgcttc acaaaatcca tttggtggac aatgcattgt
     4501 tattagcgat gatctaccgt tctaagtagt tttttctatt ggttgcattc gtgactaatt
     4561 atggtataat tatgagtaac aaagaggtat ttcattatat catcaaaccc catcagcgtg
     4621 taggctgatg gggttttttt tacgtttcta tattatagaa ttaactggta ggcttactgt
     4681 cttttttggc aattttgcaa tcaagccact tgcagatgta gtgtgcagtt acctctgcaa
     4741 atggcttgat tgcaaaaaca aaatcaaaaa ggtatttcat tatatctcct cctccccttg
     4801 ccagattagg ccggggtagc agacagcaaa actattataa cataactgaa atacctaaat
     4861 gacatataca gatatgataa tttatgtatt gatttttcta acaaaagcac atattttaat
     4921 atatactacc tattttaggt agtatatatt tttagataca atatcgatca gttttgagga
     4981 aatgaaattc tagatatttt ttgctacata gagttacttt caatattttt taacgtaatt
     5041 aggcagaagt cactcatcct ctataggttt gtgatgaaat tgctattgat ccatttaagt
     5101 aagagcaaaa aactggaggt ttatcaggta attggtctaa tggatttaag tatgcggtca
     5161 ccgtgtatag agttgcttat gagattcaag aaaataaagt tattccagta ttgctatatg
     5221 gaacacataa caatttttat gaattagccg agaacactcg caactttatt cgtgtagatg
     5281 aatcggcttc ggttgagtta taactttagc tatatcaatc aaccgaccga catatgttaa
     5341 atctatatat aataaaaata cctacttaaa tggatgttaa cggaagtgta tggtgagtag
     5401 ttcactcgta ttttgatatc gtttgtatat gttttcagcg ttctaagtgg cttattttgt
     5461 ccaataataa tatggagtgg aatcccttaa gaaacaccgt tggcaattgt cgttataaca
     5521 tgttataatt tatttatcaa aaactaaaag aagtgtgata acatgggaaa agaactaaag
     5581 gttagaaaga tcggcaatag cgtgggagtg atccttccaa gctctcttgg attaaaaagc
     5641 ggagacacta tccaagccaa acaagaaggc aacttattca tattagatac tactcaaata
     5701 gcaaaagaac acgatcgcaa attgattgaa gaaagtttcc aagactttga gaaaggttta
     5761 acagtttcag aaattgaaat ggtaaaagct ttcggaaaat acggctggag tgaatagatg
     5821 aaagagtaca cgctagttta ttctaaaagt ttccagacca gccttgaaac gataatagaa
     5881 gaatgggaaa atgagcttgc tctttctcca gccagcataa aaagatttgt aggtgcgatc
     5941 cagaaggctt tagaactaac caaaaatttt ccaactatgc atgaggaagt ttccgaaatc
     6001 tatggaatgg atatcccaac atatcggatc gtgatcggca aaagctatgc cttattttat
     6061 cggatcgacc atgaaaaaaa agagatactt gtaggacgta ttttccaatc taaacaaatg
     6121 aaactaggct tttaacaata attaggcaaa agtcgctcat cctctacagg tgggtgatta
     6181 atgccgctcg atatggttgt taaccgtcgg ttactcgaag attgatatat aagacttagt
     6241 acctttttat tttgctccat gttatggccg aaggtctaaa gatacaatca ttagtctaga
     6301 gaaaaattaa gaaccgtagg aactacgggg atcgcttggt gaataagaga cactgctatt
     6361 agtgaaaaaa taagatatca agtacgctct attcccaaaa atttcgtgac tttactcatg
     6421 agaggttcaa gtagtatatt tttaatcaaa attgacgtaa cttttaataa atactaccta
     6481 aataaggtag tatttatttt tgtgttttaa aagaggaagt ccaatatatc tatgggaaac
     6541 ttcttaaata ttatgttggt acatgaaaat atacatccct gctagactat ttacaacatt
     6601 ttactttgta cgtattcttg ttttaaaagg aatgggataa aatataggta caataactat
     6661 ttgatgctaa atataattaa tggaggagag atagtgaaca tacttaatga aaaaagatat
     6721 gtatggtttg ctggtaaaaa atatattact actttcaaaa gattatccga gttaatgcag
     6781 cagactgtta aaagtgctat gcctgaagga tacaagttta attttgttta atcttcagta
     6841 tctactgaaa gtatctattt ttcaattctt tctgagagta ggctttttca ttttagaata
     6901 agttctcatg taaagaaaac tgatgttgat atatatacgt tccgtacaaa taattataaa
     6961 aatttaaatg atttgagtag aagtatcagt gaatatctca aggggaatag ctatctcaag
     7021 attcgatttt cacatatatt aattttacta ccatatttga atgagaattt caccctaatt
     7081 caagtagata agtctttcat tgcctcaggt aatcaaggaa atatga
//
LOCUS       DAZUJO010000117         7054 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000117 DAZUJO010000000
VERSION     DAZUJO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.123"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..843
                     /locus_tag="ACG8OL_002670"
     CDS             <1..843
                     /locus_tag="ACG8OL_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFS4818590.1"
                     /translation="IKKYLEVIVQTSDVEDFFYTIKKYRDELFDWAETYDKIKNFHKQ
                     NSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKKADPYSDMMKLNELIQ
                     EFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVPIVDQEFQELKAKIEQ
                     ANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIEAANLIRETEKDEIDS
                     AAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAEINQKLKEQLQENTEI
                     TIQF"
     gene            907..4539
                     /gene="pglX"
                     /locus_tag="ACG8OL_002671"
     CDS             907..4539
                     /gene="pglX"
                     /locus_tag="ACG8OL_002671"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFS4818591.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            4655..>7054
                     /gene="pglZ"
                     /locus_tag="ACG8OL_002672"
     CDS             4655..>7054
                     /gene="pglZ"
                     /locus_tag="ACG8OL_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFS4818592.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VN"
BASE COUNT         2660 a          950 c         1329 g         2115 t
ORIGIN      
        1 ataaaaaaat atcttgaagt aattgttcaa acgtcagatg ttgaagactt tttttataca
       61 atcaaaaaat atcgcgatga attattcgac tgggctgaaa catatgataa aattaaaaat
      121 ttccacaagc aaaatagtac tcagaaaaaa atctgggaga atgcacaaga taaaattaga
      181 ttaatcgaaa aaagtcggcg tcaaacttct agccaagaaa ttgatcaatt gattcatgag
      241 atgaaaacca ttttgaaaaa ggctgatcct tattcagata tgatgaaact aaatgagttg
      301 attcaagaat ttattgaaca atataatcaa ctgttagagt tggaaagtca ggaaatatta
      361 aataaattaa cggaagaaaa acaagcaaca tttaaattat tagaaggaaa agattttaag
      421 gatcaatttg taccaatagt tgaccaagaa tttcaagaac tgaaagcaaa aattgaacaa
      481 gcgaatacta tagatgatct tgttttctta tcattaaacg tagcagaaca gtatttgatc
      541 tttaatcaac gtttcgataa tgaagaaaac cgcagacgtc aagaagaaca agcacgtaaa
      601 caaattgaag cagcaaactt gattcgtgaa actgaaaaag atgaaataga ctctgcagca
      661 aaagctgaag aaatagcgat agtaaaacct actattcaac agaaaacagt accaatgaga
      721 acacttaacg ttcatgttaa aactattaag tcagcagcag atattgatga gttaacagct
      781 gaaattaatc aaaaactgaa ggaacaactt caagaaaata cagaaatcac gattcaattc
      841 tagtgaaaag acaggcataa aaaggtgcct gtcttttcaa aggttacata tagaagggaa
      901 agaaaaatgg ataagaaagc gataaagaat ttcgcggtca atgcccgtag aaaattaatt
      961 gatgaagtaa tgactaaagc aaaacgttta ggaattgatg aatcaaaaat tgaagatgtg
     1021 aagaaaattg atagcaattt acaagaaatc gaaaaatcag gaataagaat tcaaggaaaa
     1081 gaaatagcgc aaagaaacaa attgattgca gaactaaaac aacgtgcttc tgtcacttct
     1141 tattcagaag cttttcaaga gttaatcgaa gaggttgctt atacgtggtt taaccgattg
     1201 attgccattc ggtttatgga ggtaaatgat tatttacctg acacgtatcg tgtattatct
     1261 agtgaaataa gtgggaaaat tgagccagat attattacag atatttatga tgccgatatc
     1321 tttgaagaat tatctagtaa tgaacagcaa caagtcagaa cttggaaaac agataattca
     1381 gcagaagcaa tggacaagct gtatcaattg atttttataa aaatctgtaa tcaattgaat
     1441 gattacttac ctgagctatt tgaagaagta gaagattata cagaactttt gtttacagct
     1501 tcttatatta aaaaagatgg tgtaatcgct gatcttttaa gtattccaga ggatgatttc
     1561 tatgttcaag ctggtggaca ggtagagatt attgggtggt tgtatcagta ctataatagt
     1621 gagccacata atcaagtggt gaatatttat aaagggattg tcaaaaaaca agatattccg
     1681 gcagcaactc aactatttac cacagattgg gtcgttcgtt atatggttga caatagttta
     1741 ggaaagtatt ggttagatca ttatcccaat agtgaattaa gaaataagtt gaaatactta
     1801 attccagata aatatgcttt tgaacaagca acatctgtaa gtaatttaaa accagaagaa
     1861 ctaaaaatta tcgataacgc aatggggtct gggcatattt tagtttacgc cttcgatgta
     1921 ttgatggaaa tttatcttac gttaggatat tctgagcgtg atgctgcacg tgcaatcgtt
     1981 gaatacaatt tatatggtct tgaaatcgat aaaagagcgt atcaattgtc ttattttgca
     2041 attatgatga aagggcgtca atacgatcgt ctttttttga aaaaaagtct atcgccgaat
     2101 ttaagagagt ttattgattc tcctaaagtg acagacgagt atcaagagcg tattagtgaa
     2161 ttgaacaatg ataagtatag tgaacgctgg aatgaataca aagagttaat ttttgatcaa
     2221 tttgaaaatg ggaaagaact tggatcaatc attaagttag tcgataaaaa taatcaggca
     2281 ataactaaag aatacttagg aaaagtaaga gagtatatat caaaattcaa cgaatttacg
     2341 aatatggata ttctttatgg gatgaaggaa atacaagaaa aatacttgca cattcttgac
     2401 gtggctgaaa tgctagttga taaatatgta gcggtagtca ctaatccgcc ttatcttaat
     2461 aaaatggata agaaattaaa aaaatatgta aatgataact attctgatgt caaaaaagat
     2521 ctattttctg tgtttattaa attgaatagc caaatgctga ttgataatgg atatgcttcc
     2581 tttatgacgc catttgtttg gatgttcatt tctagctatg aaaagttacg ctcattttta
     2641 attaatgaaa gaaacatttc tagtttgatt caaatggagt attcagcttt tgaagaagca
     2701 accgttccaa tttgtacgtt tacgataaaa aattcaaatg aacaagatgg aagctatata
     2761 aaattatcag attttaaagg tggaatggaa gttcaaaaaa ttaaaacgtt agaagcaatt
     2821 catcagcatg gtgaatgcga ttatttttat agtactaatc aagacaactt caaaaaaatc
     2881 ccaggaatgc cgattgctta ctgggcaagc gataacgtaa tcactgattt tgctcaagga
     2941 aaattaacta acgatgttgc agatgcaaaa caagggttgg caaccggaaa taacaataaa
     3001 tttttgcggt attggtttga agtagaattt aacaaaataa agtttgatgc taaaacatta
     3061 gaggacgcta aaaagtctag aaaaaaatgg tttcctacaa ctaaaggtgg tcgttttaga
     3121 aaatggtatg gaaacaatga atacgtggtt aactgggaga atgatggaga tgaaataaaa
     3181 aattatgtta atgaaaatgg gaagttaaaa tctagaccac aaaatcttaa ttactatttt
     3241 aaaaagggaa ttacttggtc gacactttca tctggacaag tatcttttag attgcagaat
     3301 caggcaattt ttgaaactaa aggatctgtt ctgttctcaa aggatataac tgactttgaa
     3361 gaaatatttg gctatgtaaa ctctaaatta gttaatcact ttttaacttt tatttctccc
     3421 accttggatt accatgaagg accaatggga aaactacctt ttcttaaagt tacatctgag
     3481 agaaaattaa aaaaaataag agaaaaagtg gatgaaaata tcgatatagc tagaaaagac
     3541 tataatgctc ttgaaacgtc atacgagttt agaagaaaca ttttgttaag ttatatgttt
     3601 aaaaaagatc tctcagaggc atttaactgt ctaagtaggc agttggttaa tcaatttaat
     3661 actctaaaat cgaatgaaga ggaactgaac gacatattca ttaatcatta ttctcttgaa
     3721 aaagaattaa caaaagaggt tcaagatgaa tatatctctg caaggttacc tgataaggcg
     3781 agggaaatta aggattttat ttcttattca ataggttgta tcttcggtcg ttactcccta
     3841 gacgaagatg gtctcatctt cgctggcggc gaatgggatc cttcccgcta tcaaaaattt
     3901 aaacctgata ctgataatgt tttactcatc accgatgatg agtatttcaa ggatagccaa
     3961 attgacattg tcaatcgttt cattgatttc gttgagatta cttttggtaa agaaaccctt
     4021 gaagaaaatc tccagtttat cgctgacact ttaggtggca aagggacaag tcgagaaatt
     4081 attcgtcgtt acttcgtaca agatttttac aaagaccatt taaaaacata ctccaatcga
     4141 ccaatttatt ggcaattgga ctcaggaaaa caaaatggct ttaaagcgtt aatgtacttg
     4201 catcgctata caccagatca attaggaaaa gtacgtactg aatacctaca cgaactacaa
     4261 aaagcgtatg acaatcgaat cgaattgcgt cagcaacaaa tttcaacaag tgataaccca
     4321 aaagaaagaa atgcaatgac aaaagaaatt gtaaagattc agaagcaatt aaaagaagtt
     4381 cgtgagtatg acgagaagtt gggtcactta gcattaaaac gaattgaatt agacttagat
     4441 gatggtgtaa ttgtcaatta tgataagttg cagcgtgatc ctaaaaccgg cgagaaattc
     4501 caaatttttg caaaaggggt caaagaacca aaaaaatagt tatctaagaa agttgttttc
     4561 taacaacttt cttttttatt cttaaattgc gtgcgaaatt atccaaatta tgtcaatata
     4621 aggggtatag aaagagaacg attaggtgag attgatgaca gatataaata cgcaacaaat
     4681 tgaagaaaaa ttaaatcaac gttttcctaa ttatggatgt aggaaaatca ttttttggtt
     4741 tgattcaaat caggattttc ttgaagaaat tgactcattg gtcattcaaa atgcaaaaat
     4801 ctatcgatta gaaaaggacg cacaatttaa aactaagcgt ttattggaat atgaagatcc
     4861 cgaaactaac tatttaatct atgcgccatt tcctcgttgg gatgataaag atgaagataa
     4921 tcatttgttg agtgtattga attattcaga ggaatttagt gcagaccgct tagcgattat
     4981 catgaatgaa ttggctattc cacttagatt tcatcagaca gttaaacagt atcgtaattt
     5041 ctttaatgca aaggatcgca tgagaagttt tggtgaattt agtacgcata tgacaattaa
     5101 taaatcagaa acgattgaat taatgataat ggctgttttg ttgaaatcga aaagtattcg
     5161 attaaatgat ttactgcgtg tagttctaaa aagctttaca gaaagtaggg agaatccttt
     5221 aagggaactt gataagtatc agttaacagc taaattttgg gaatttattc aaatgaaatt
     5281 tggctttacg gatagccagc caacattgga aaagctgacg atgtgtcttt atttgaacta
     5341 tttttattat caaattgatc aagagatgcc gaagaagttc aaaggatttg aaatactaca
     5401 tttgaaagca aacattatta ctttcatgga tcagtttatg aatgatacac gttacatgga
     5461 aagctttgaa cagctatcta aaaaagtata tcaggctatt gatggtgatg gattattaga
     5521 cagtatagaa attgatgatc taattgaagc agacgtattt tctgagattc atgcaaaaat
     5581 attaatgttt tatgcagaac gattaaaggc ttctgacact acaacgaaaa ttgcaggaca
     5641 aacaattttt gatagtttaa agcagcgcga aaaaatgcat ttcgcatcgg attatattta
     5701 tgagtatcaa ttattgaaac atgcttatta tcttgtggat aaaaacagat tgtgcttgcc
     5761 gatgaacatg gataatttgc taactcaata tgagaaggaa atgtattggg ttgatacgca
     5821 ttatcggaaa tttatttgga atagtgatca tatagaagaa aagcaaaaat atggccaatt
     5881 gtatcgatta gttgaagcaa attattcgaa gtttcttgat caatcaggac aaatttggaa
     5941 tcaaactttt gaatttaaga atcgtccatc cattcgaaaa ttttataatc aagctgtaat
     6001 gaaacgctct gttaaaactg tagttattat ttcagatgct tttcgatatg agttagggat
     6061 gaatttagaa cgtaagttag ttagagaaaa gaagtattca acaaacatgg ctagctttct
     6121 ttctgtatta ccttcagtta ctgaattcgg taaagccgcc ttattaccaa acaatcaact
     6181 aacttacaat gaaggaacag aagtactgat tgatgggaaa aaaacgaatg gtttatccaa
     6241 tcgaaatgcg attctccagc aacgagatgc aaatgctgta gctttaaatt atgatgatgt
     6301 ggttaagatg aataaggctg aggtacgaga agtatttaat ggcaaatcag tcatttatat
     6361 ttatcacgat caagtggata gaacaggtga tcactcgaat gaaaatcgag tttttgaggc
     6421 ctgtgattta gcgataagcg aaatttcccg acttattaat cgcttgcata atgatggcag
     6481 tatctatcgt tttatagtta cggctgatca tggatttatt tataaaagaa gccacgttga
     6541 agaagtagat aagattgaaa atccatctag aaatgaaaaa gatcgtgtag aacgtcgttt
     6601 tattcttagt gaagaagaat acgaaataat tggtacgaaa aattactctt tgggagattc
     6661 tttaggaaat gatgacacta gaaacatttg tgtcccgatt acttcaagta tttttaagaa
     6721 agctggcggg ggacaaagct acgttcatgg agggtcatct attcaagaaa tgctagtccc
     6781 tgttctagaa attacggtag ccaaaggtag caatgcaaag gaaccagtag caattgaatt
     6841 aatgactagc aaccgcagaa ttacaggatt atcaaccacg ttagaattct atcaaaaaaa
     6901 tgcaattagt gatgtttatg accccgcatc tttctggctt tattttgaag atgaatatgg
     6961 tgaacgtatc tcaaatgaag aaatctatgt tgcggattca aaaacagaag atgcaaatca
     7021 gcggtttact aaatttgttt ttgaatttgt aaat
//
LOCUS       DAZUJO010000118         6741 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000118 DAZUJO010000000
VERSION     DAZUJO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.124"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..608)
                     /locus_tag="ACG8OL_002673"
     CDS             complement(150..608)
                     /locus_tag="ACG8OL_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS4818593.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(621..1952)
                     /locus_tag="ACG8OL_002674"
     CDS             complement(621..1952)
                     /locus_tag="ACG8OL_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS4818594.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1963..3405)
                     /locus_tag="ACG8OL_002675"
     CDS             complement(1963..3405)
                     /locus_tag="ACG8OL_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS4818595.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3392..4156)
                     /locus_tag="ACG8OL_002676"
     CDS             complement(3392..4156)
                     /locus_tag="ACG8OL_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS4818596.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4260..5135)
                     /locus_tag="ACG8OL_002677"
     CDS             complement(4260..5135)
                     /locus_tag="ACG8OL_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4818597.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5390..5474)
                     /locus_tag="ACG8OL_002678"
     tRNA            complement(5390..5474)
                     /locus_tag="ACG8OL_002678"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5438..5440),aa:Leu,seq:caa)
     gene            complement(5492..5563)
                     /locus_tag="ACG8OL_002679"
     tRNA            complement(5492..5563)
                     /locus_tag="ACG8OL_002679"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5529..5531),aa:Gln,seq:ttg)
     gene            complement(5573..5645)
                     /locus_tag="ACG8OL_002680"
     tRNA            complement(5573..5645)
                     /locus_tag="ACG8OL_002680"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5610..5612),aa:His,seq:gtg)
     gene            complement(5707..5791)
                     /locus_tag="ACG8OL_002681"
     tRNA            complement(5707..5791)
                     /locus_tag="ACG8OL_002681"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5755..5757),aa:Leu,seq:caa)
     gene            complement(5812..5882)
                     /locus_tag="ACG8OL_002682"
     tRNA            complement(5812..5882)
                     /locus_tag="ACG8OL_002682"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5848..5850),aa:Cys,seq:gca)
     gene            complement(5928..5999)
                     /locus_tag="ACG8OL_002683"
     tRNA            complement(5928..5999)
                     /locus_tag="ACG8OL_002683"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5965..5967),aa:Gln,seq:ttg)
     gene            complement(6011..6083)
                     /locus_tag="ACG8OL_002684"
     tRNA            complement(6011..6083)
                     /locus_tag="ACG8OL_002684"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6048..6050),aa:His,seq:gtg)
     gene            complement(6086..6159)
                     /locus_tag="ACG8OL_002685"
     tRNA            complement(6086..6159)
                     /locus_tag="ACG8OL_002685"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6125..6127),aa:Trp,seq:cca)
     gene            complement(6166..6246)
                     /locus_tag="ACG8OL_002686"
     tRNA            complement(6166..6246)
                     /locus_tag="ACG8OL_002686"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6210..6212),aa:Tyr,seq:gta)
     gene            complement(6248..6323)
                     /locus_tag="ACG8OL_002687"
     tRNA            complement(6248..6323)
                     /locus_tag="ACG8OL_002687"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6288..6290),aa:Phe,seq:gaa)
     gene            complement(6359..6431)
                     /locus_tag="ACG8OL_002688"
     tRNA            complement(6359..6431)
                     /locus_tag="ACG8OL_002688"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6396..6398),aa:Asp,seq:gtc)
     gene            complement(6441..6513)
                     /locus_tag="ACG8OL_002689"
     tRNA            complement(6441..6513)
                     /locus_tag="ACG8OL_002689"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6478..6480),aa:Val,seq:tac)
     gene            complement(6529..6600)
                     /locus_tag="ACG8OL_002690"
     tRNA            complement(6529..6600)
                     /locus_tag="ACG8OL_002690"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6565..6567),aa:Glu,seq:ttc)
     gene            complement(6615..6704)
                     /locus_tag="ACG8OL_002691"
     tRNA            complement(6615..6704)
                     /locus_tag="ACG8OL_002691"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6668..6670),aa:Ser,seq:gga)
BASE COUNT         2041 a         1480 c         1093 g         2127 t
ORIGIN      
        1 taagttttct aaatcatagg agcaacaaaa aaattcgata attaacaaaa atcaaaatga
       61 ttaaatctag tgattgaaac atatcatttc aaaaaaattt tgtaaattca cccataaata
      121 tcaccctatt taactttttt catcgtacac taaaattcaa aacaactatc tttaatcttt
      181 acttcttggt tttgttttaa taaatttcct tcaaaaatgc ctggagaaac tacaatgatc
      241 accgtatctt tttgtgcttg ctccaacttc ttcaaatcta taatagctaa cagtgtccct
      301 tcttttacct cctggttact ttcaacccgt atctgaaacg gacttccttc gagttcaact
      361 gtatctaaac ccatatggat cagtattttt ctgccagatt cactttcgat cgttatggca
      421 tgtcgggtag gaaatatatt atcgattatt cctttaactg gcgcatggat atatccatca
      481 tgattcgtta tagcgtatcc aactcctatc atttttgaag aaaagacctc gtcttttact
      541 gctgttaatg gaataatttg accactagct acagctttta gaatgtttct tgttttaaag
      601 aaatgcactt tttatcacct ctactttaat gctggccaat attttttgtt tactttgatc
      661 atttcatcta gtattttttt tgcgactctg gcatttggta cggtttgatt caatgtaaat
      721 gcttgtaaag ctttttgata agaaccttca aagtaagcat ctactagtaa cttctctact
      781 gcaacttgtg cctccatcaa tcctttatga aaatcactga ttggttttct tagtgtaatt
      841 ggctccacac cagttgcacc aacatatgcc ggtatttcaa caactgcatc tgaccgtaga
      901 ttcggaatcg ccccttgatt aggaacgatt agcataaagc gacgacgatc atcattcaac
      961 aaggaaatag ctatatcgac gatatattgt ccatgctccc caaaattaaa gttcaatact
     1021 tcttccacaa taccgcggcg tattttatca gccatttctt tggtattttt ctctcgtcca
     1081 tccatgacca tattagcacg tgtatactct ttgtcagtat gctcaacgac catatcagga
     1141 tataagtaat actctaagta attattcggg atattacttg gaaaattttt cgtgatgact
     1201 gacatcattt gaaaggtttt ttgccaagtt ttatctccta tattaaaatc ggcgacttgc
     1261 atttcttttg ttattagttt ttcaataatc tctggcataa tttctcgttg caattcttta
     1321 tcataaatcg atgtatacca gccaaagtgg tttaaaccat aatacgtaac gatccaattc
     1381 ttacgatcat aaccgtaatt cactgcaatc gtttcttcaa ttgaaatagt catatcacag
     1441 gcattaatca tttttacatt cggatattta cgacgaacag cttcagaaac gatcgactct
     1501 ggatttgtat aattcaaaat ccatgctttg ggggcatagt cttgtatatg atcgacaatt
     1561 tccaataaac ctttgattga gcgcatccca taagcaaatc ctccgagtcc acacgtttct
     1621 tgtcctacta atccatgttt aagtggaatc ttttcgtcct tttctcgcat ctccaatcct
     1681 ccaacacgaa tttgtgagaa aacaaaatcg catcctgtaa aagcaagctt tggatcatcc
     1741 gttgcacgaa tgcgaatatt tgaaaaacct tttcttttca gcataaattt aacaatcaag
     1801 tacatatcca tatttctttc ttcatcaata tcatataaac gtatttcaga taatggcaac
     1861 cgtttttcat tgttcaaaat ggcttgaacg attcctggtg tatacgtact cccgcctcca
     1921 gctatagtga taatctgttt tttcatcatc atcttttcct ttctattctt ctaataattt
     1981 attgaattct gccataacat aagtcacttc taagccgatg ataatttgat aatttttttc
     2041 agttggttta atgatcccgc ttatccctaa ttgcttgatt cgctgttcct cgaccatggt
     2101 gggatccact acctctaccc gtaggcgtgt attacaacta tagacatcca ggatattggc
     2161 ttttcctcca aacgcttgta gcatttttgt tgccatatat tcataatttt tacttgctaa
     2221 ccctaactct gtttcttcta aggttacttc attgccaaac tctgccttag cttcacgtcc
     2281 taatgtctga atgtttctct tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa
     2341 aaagaaaatt ccgattggaa gaatcaaaat agcattggta gcgattcgaa aattgatgag
     2401 ataatcaaca atacatgctc cgaatgtaaa accaattcgt atattgaata agtaacagac
     2461 aatccctgct aaaccggcaa aaagtgcatg gatcacgtac aacataggag aaagaaacat
     2521 gaaactaaat tcaacaggct cagtgatatt tgcaataaat gaagttcctg caccgcttcc
     2581 catgattcct ttgaccatct ctttattttc tttataagct gctttataga tagctagaca
     2641 tatagctggt ataccaaaca tcattactac aaaaaaaact gataaaaaca gtccagcctg
     2701 aggatctccg ttgataaagc gtgtcatctc tcctctgaaa acttccttgc taggagatgt
     2761 atagcttcct aaatcgtaat aaatatacgt attcaacaca tggtgcagtc caaaggggat
     2821 cagcaaacga ttcaaaaaca taaatatccc cacacctaat gctcccatat caacgagcag
     2881 ttttgcaaaa ttatcaatcc caatttggat gagcggccag ataaagccca aaaaaacagc
     2941 tgcgattgtt tgtagaatca tcaccatagt caatgggaat ttttcgcccg caaaataaga
     3001 aaaaactact ggtaattttt gttctttaaa gcgattatac gtccatgccg ctactaaacc
     3061 ggaaataatt ccaccaaaaa tccccatatt cacttctggg tccataattt ctaagccttt
     3121 tttcaaggta gcaatcgcta aaaaaccagt aagtgcaggt attcctttgt ccttattttt
     3181 gacaaaaccg attgcaactc ctatagcaaa taagatatcc aagtttccaa aaacggcatt
     3241 tccagctgca ccaattactg ggatgttcag catatcgtca gctgataaac gcatgatcaa
     3301 tcccgctaat ggcatcgctg caactggaag tagcatcgct cttccaagct tctgcacatt
     3361 tgatttaaca ttcattttta ttcctcctct attaaatatc ctcatatgtt actaactcaa
     3421 tcaaaggatc atttttcact ttttgagcaa atgctgaatg aattaaaaat tcgacttggt
     3481 gggtacgagg atacacaaaa cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt
     3541 gacacatgac ttcgatcgtt ttatcttctc tcaatatcat gagtaaactt gccaaatatt
     3601 cttccacact cgtattgatt tgtcgttttt tttcatcagt ttctcctacg acatcgaaac
     3661 aaggctcaaa ataatcgaca tgacgaactt catcttttcg ttcaaagttg ctccgaaccg
     3721 gcaaatcata tttttttgct aatgtaacaa ctacctcctg attttcatcc aaagtatgag
     3781 tatgatgatg actgtctaaa tgagttggta tgatccccgc atgataaatt ttttgaatct
     3841 gagcattcca ttcctgatac agttgatctt tgtcaatgtt aaatggatgc tgataataag
     3901 ataaggatct gaactctccg ctttcgataa gagtatcgac agttttgagg agtggtttgc
     3961 cacacgtcaa tgttaaatgg actcctacac ctagcctagg catttccttt cttagtttca
     4021 cagcatgctc gaaacccggc atatttgcca tcaatgtagt tgatgtcaaa attcctcgct
     4081 gataagcatc cataatccca taattcacac catggctata gccaaaatca tccgaattaa
     4141 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
     4201 atcatagtcc atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt
     4261 taatagattc ttgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt
     4321 tgaaattgct gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt
     4381 ttaaatgttt cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag
     4441 cgtttaacca ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca
     4501 taaacgtttg aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt
     4561 tttaagctct ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg
     4621 aatcctactt catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact
     4681 tctactgcta tatcatattc atttggataa aggaatctca tttctggcaa cataattgct
     4741 gtgacatctc tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta
     4801 atatagatat ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct
     4861 tccgcaaaat ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc
     4921 ttttctttag aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc
     4981 actttagcag aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg
     5041 atcaatacag cattatgatt cagctttttc atcacataca ccgttaattc acctcctcta
     5101 tatgataaaa caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt
     5161 ttaaagacaa aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt
     5221 agcaccctac ataatttaca aaaatagttt aacacataat aaaagcgtta tcaatatctt
     5281 ttttactcgt tttatgataa tacttgtatc gaattgattt ggaggtaatt acataaaaac
     5341 aaaaaaaata tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc
     5401 ggggtcgaac cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat
     5461 tccgccacgc cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat
     5521 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa agggcgaatg
     5581 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
     5641 ttcgccatca taaaagatgt tatttaattt ctcaataaaa aactaagatg aagacatctt
     5701 agtcaatacc ggcggccggg gtcgaaccgg cacgccctca acgggcactg gattttgagt
     5761 ccagcgcgtc tgccaattcc gccacgccgg caagttgatt tattagtact aaggcggtaa
     5821 ccggatttga accggtgatg aaggttttgc agacctctgc cttaccactt ggctataccg
     5881 ccattatact attatcaatc tataaaataa agaataaatt atagaaactg gggtagctgg
     5941 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     6001 gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc
     6061 gttaaccact tcgccacatt cgccatggca ggggcagcag gaattgaacc cacactaacg
     6121 gttttggaga ccgctgttct acctttaaac tatgccccta taaaatggag gagagtggat
     6181 tcgaaccact gaaccctaag gaacggattt acagtccgtc gcgtttagcc acttcgctac
     6241 tcctccatgg tggcgcggga cagaatcgaa ctgccgacac acggagcttc aatccgttgc
     6301 tctaccaact gagctaccgc gccatatttt aaaattatta attgaataac aagaacgacg
     6361 gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac
     6421 accacggaac cagttcatta tggaggttaa cgggatcgaa ccgctgaccc tctgcttgta
     6481 aggcagatgc tctcccagct gagctaaacc tccaacattc aattaaaatg acccgtacgg
     6541 gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc
     6601 aggtaaattt gctacggaga gtaagggatt cgaacccttg agacagtttg gcaccatcta
     6661 catgatttcc aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc
     6721 aatactcttt ctcaaaatga g
//
LOCUS       DAZUJO010000119         6669 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000119 DAZUJO010000000
VERSION     DAZUJO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.125"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            277..873
                     /locus_tag="ACG8OL_002692"
     CDS             277..873
                     /locus_tag="ACG8OL_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4818598.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(931..1413)
                     /locus_tag="ACG8OL_002693"
     CDS             complement(931..1413)
                     /locus_tag="ACG8OL_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818599.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1519..1716)
                     /locus_tag="ACG8OL_002694"
     CDS             complement(1519..1716)
                     /locus_tag="ACG8OL_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818600.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2011..2769
                     /locus_tag="ACG8OL_002695"
     CDS             2011..2769
                     /locus_tag="ACG8OL_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS4818601.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2829..3257
                     /locus_tag="ACG8OL_002696"
     CDS             2829..3257
                     /locus_tag="ACG8OL_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818602.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3303..3375
                     /locus_tag="ACG8OL_002697"
     tRNA            3303..3375
                     /locus_tag="ACG8OL_002697"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3336..3338,aa:Gln,seq:ctg)
     gene            complement(3525..3896)
                     /locus_tag="ACG8OL_002698"
     CDS             complement(3525..3896)
                     /locus_tag="ACG8OL_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS4818603.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4111..4296
                     /locus_tag="ACG8OL_002699"
     CDS             4111..4296
                     /locus_tag="ACG8OL_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818604.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4379..4567)
                     /locus_tag="ACG8OL_002700"
     CDS             complement(4379..4567)
                     /locus_tag="ACG8OL_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818605.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4591..4854)
                     /locus_tag="ACG8OL_002701"
     CDS             complement(4591..4854)
                     /locus_tag="ACG8OL_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818606.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5012..5269
                     /locus_tag="ACG8OL_002702"
     CDS             5012..5269
                     /locus_tag="ACG8OL_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS4818607.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5340..5531)
                     /locus_tag="ACG8OL_002703"
     CDS             complement(5340..5531)
                     /locus_tag="ACG8OL_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818608.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5672..5992)
                     /locus_tag="ACG8OL_002704"
     CDS             complement(5672..5992)
                     /locus_tag="ACG8OL_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818609.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6015..6449)
                     /locus_tag="ACG8OL_002705"
     CDS             complement(6015..6449)
                     /locus_tag="ACG8OL_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS4818610.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2090 a         1300 c         1019 g         2260 t
ORIGIN      
        1 aatttttaat ggcaatatca taaaaaaaaa gaagatagag atttaataat tatatatcag
       61 ctgttttttt caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa
      121 aatccttaca taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt
      181 gagcatatct gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa
      241 aaaatgcaaa acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg
      301 aaaaaccgcc gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta
      361 acaactttga tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca
      421 cgcgcagtta tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca
      481 cttcgcccat tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc
      541 aaaaatggtt atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa
      601 gaacaatttg aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc
      661 gcaacagcta atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat
      721 tacaacccag ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa
      781 ctacgttcgc aattagtatt cggttcacca gaaacaccgg ctgaggaaaa agaatacatg
      841 aacgatgccg atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt
      901 gtgacaaaca tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc
      961 tgcctcatcg tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa
     1021 tttcagcatc ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc
     1081 ttttggctga tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa
     1141 gctattcaaa acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa
     1201 aatcattgct gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt
     1261 ataccaaatg actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac
     1321 agtccgataa ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa
     1381 taaaaacaat agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc
     1441 agaaaatagg aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg
     1501 cacgtccaat tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt
     1561 ttcatctaat aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt
     1621 tgtcattttt gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc
     1681 atattgttct cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa
     1741 cttttctcca cattaccact gctatttctt tggagattta actcattgat ttttatttac
     1801 agttaaaaat caaccttaca ggcattatag cgtactgttt tcgttttcac aattaaaaca
     1861 gctgttgtat ttagaagaat ttttttcaaa aaattaaaac atattcctta aaaactaatt
     1921 atttcctaca tattaaaata taatacttcc atttccaata aaaatacatt tataatagga
     1981 aagataaaca gctgaaaagg gagatttcaa atgaagaaaa aaacgttcaa aaaactattg
     2041 tttctagggg gcacagctgc tcttttatta agcggtacaa gttattacct acatatccat
     2101 tctccagaac atacaatgaa cactgcacaa aaaagttcaa cacaaaaaac gaaaaagaca
     2161 aaagagaaag cagaagtttt actagatgtt ccattagaga gccagtttga ttctccttct
     2221 ttagaaaatg gctgtgaagt cacttcgctt tctatgcttt tatcttttta tggttatgaa
     2281 acaactaaaa accagttagc tgagcaattg gattacgtcc ctgttttcaa tgcagatggg
     2341 actcatggcg atcctaatga aggatttgtt ggtgatatca gtggcggaga ctgggctatg
     2401 gtcgtctatg ttcccccagt tgcctccctt gctcagcaaa tcgtccaaac ggattatcac
     2461 acttttccaa aaaccgatgc tcaactcgat gatatcaaaa aagcactatc taaaggaaat
     2521 cctgtctgga cttctgtgac tatcaatttt gaagtcccag atgaaactga ttttatgact
     2581 tggaccacaa ataatggaga agtaagagtc actcctcttg ttcatgcttg tgtagtcact
     2641 ggatatgaca aagaaaatat ttatgtcaac gatccctatg gtgtaaaaaa tcgagcagta
     2701 ccgatcaacg atttttcggc gattttcact gcaatgggtg gacagatgct aactttggag
     2761 aaaagctagt aattcaaaca aaaatccctt atactcaaga taccaaacct ttgaagggag
     2821 cgtagataat ggaaaaattc acacataaaa aaatggatcc aaatgaaatt ccgattattt
     2881 ttgtccgtga ctgcaaggga aatgttcaag gtaaagtctc tattaatgag tggaacgagc
     2941 gtcgcagacc tgctaccctc aatgaactgg aaatcaaatt gtatcgtcaa tctcttgttt
     3001 attacgcaga tcaagagtac gagaaagcca ccgatttatt gaaatttcta attgctcgaa
     3061 cagagtatac tcgctttgaa tatattgaac gtttagcaaa catctatcat attatgaatg
     3121 aacctgtcaa agaatatcag ttattggaca ccgtcttgtc agtagcagaa ctgattgccc
     3181 ttcctgctgg attagaaaag aaactagtac gccgcctttt acgagtaaag caacaactat
     3241 ccgatcaaga aaaataactt gcgttttttt aggaaagtgc tatgattatt ttgttgaata
     3301 aatgggatgt agccaaactg gtaaggcact tgactctgaa tcaagcaatt gtaggttcga
     3361 accctgccat cccaatttga tgtgcttgct gaaaaaatga accccctgta aaagaacaat
     3421 ctttttgcag ggggattatt gtattatttt tactacttgt atttagttta cataattaaa
     3481 ttatgaataa taaaatcggg cgtattctta atacaaaaac tgatttaccc aataattgct
     3541 aatgctttct ccggaacttt ttctctagga acttcctcat acgtttctat gtgtgctcct
     3601 ttattgctaa ttttcaaata atgattttct tttaacacag aaagaccatt aaattcaacc
     3661 gttcgttctt tcccattggc atcagcagct ttctgcacat accttgctgt accatattca
     3721 tttacttcct ctggtttttg tgtctttaca tagacctctc ctttttcaac taatggattt
     3781 agctgatcaa gtataccagg gatttctcca taacttcctt cagtataata tttcagtcca
     3841 aatagcaaaa taccactaat cgccgccagt ccaatcaaat atttaatcac tttcatcttt
     3901 ctacctcctt gttatatatt cagtatatgg gtcagttaaa gaaattccat ccgtcccaag
     3961 ttggagattt ttctcctact ttagaagcga gaatcgagtg tttttttagt aaaaatgatc
     4021 gttatttgat attattgacg tgtggataaa tcagtttttc tcgttttgtc catgtaaaaa
     4081 atcatgttga tagcaaagga tgtgttcaaa atgaatcaag aagaattatt tcaaaaagtc
     4141 aaggaaatga ttaagaacgg caatttcgat ggcgcaaaac gatttatcga agaacataaa
     4201 gaacaattag gaccttataa agagaaagcc caaaatcttt taaaagatgt aaatatcgac
     4261 agtgtcaaaa acaaatttaa aaatctattc aaataagtaa aactttcact tttgtaaaat
     4321 caaaggagga caatgctgct gcatcgtcct cctttggttc ttttctgaat acttcatact
     4381 aaaataaacg tgtcggatcg taatgataag tcacattgcc gttcagtgag gcttttccca
     4441 ccacatattt acgattctcc aacttcagcc acgcttctcg aaaagccatt aattcttctc
     4501 tttttacatc aatcttttcg atttttccat cgatcaaatc ttgaataagt tgttcatatg
     4561 gattcatttt tttcctccat ctgctgtcaa tcaataagtt gtaaaaatag gttcatatgc
     4621 ttttttggta tctagatctt ctctgacttt gactgtcgta taaataattt tttctactgg
     4681 gatactataa taacttcctt caccattatt gaatcggtga acgtctttct ttgctaaaat
     4741 accatttatt ttattcgtca gtacacgttg ttcgacactg ttacttacaa catagtaaat
     4801 cacccgaata ttattgatca aatgcaaatc gatttctaac actacatgat ccatgaaaat
     4861 cccactcctt ctttcacttt gattatagca agtttctcct ctaatttcaa aatcgataca
     4921 gattttaccg gataaatcca caaaactgct tgcgccattt cgcacaaagt gttattttgt
     4981 atcatggact ttatttatcg gaggcaatag tatgatcgcg atcggtcaat ttgtttttta
     5041 tattcctttt tttattatga taagcattct tttctattac ataaaatgga caaaaaagaa
     5101 gttttcagtt ttactggcct ctttacctgc tgtatatttt acttatcaaa ttttttcttt
     5161 taggcattgg gaaacaacgt ctgtattaat aacacacatc atcgaattga cgctttcagt
     5221 aatttttctt attatttgga tctatttttt atataagaat cagaattgaa tccctcgcat
     5281 gtttaaaaag aaaacgatta aagatgcagc aaatttctgc cgatctttaa tcgttttttc
     5341 tattctgtta taccaaaaaa atgtttgaat cggtgaagga tcgttccttg ttttaattct
     5401 ataaattctt gtgcgatcgg gtacttttct tccaaatttt ctttgttctt ttcgatcagg
     5461 cttttcgctt cttcatattt tttatctgca catagtgctt ttacttcctg taatactttt
     5521 cctgcttcca tttgcctcac cctcttgcca ttttattgct ttaagtttta tttaagttat
     5581 acgcattatt ctagtttcat cccaaataac cttttttcat ttttactcct cgaactaatc
     5641 agttttctaa aggctgattg gttcgtcttt tttatttttc ctccatttca ttctgctctt
     5701 ctgttaattt atctaatgct ttgattgatg ctgcggctct tgcataatca tagctgtata
     5761 attccataaa tttatcaggc agaacaaacc acgctggatg ataaccgtta gctgtgacag
     5821 ggaatccttt atcttgttgg ttatctccat cccatctcag agcaacacct ttttttcctt
     5881 tccactttac taaagcgatc gagtatccct tctttgtttg tgtgccgtct tcaataattg
     5941 ctaaaatttc tattttttct tttggcgcac ggacttcttg aggacgaacc attttctatt
     6001 cctcccttat ctcattactt cacgaccaac acactgcaag gggcatgtga tacgacataa
     6061 gaagcggtcg atccaactaa tgcttgttgt atagcacctt ttcctgtcgc tcccataacg
     6121 atcaaatcaa tattttcttg tccagggatg acattggcaa tcaaatttct tggatctcca
     6181 gtttcaacta ttgcgtggat ctcagcaatg ccgtactctt tggcatcatt gatttttttc
     6241 agcatggcta cttctgttct ttgtttttca tctgcataga tttttgaaaa ggcataggca
     6301 ctatgggtca gctcaacttt gttgatgatt gacacgataa ataagtgggc ttcattgtcc
     6361 tttgctaagt caagggcttc agcgaacgct ttgatcgatt gtcttgatcc gtcaactgct
     6421 accatgatgt tctggtatct tttactcatt ggaaccgcct cctttttctt ttattgtaac
     6481 atctcaagca aaaatacaaa aaatatacgc aatatgtaaa taaaatgatg aacaaaagca
     6541 atttctttca agttatttat acatttcttt tctcatcaga acacctgttc taatcccatt
     6601 atttctgcat tatgtgaaat tcctcatcat ttttcacatc gagcgaacat cattctgtta
     6661 caaaaaacg
//
LOCUS       DAZUJO010000120         6473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000120 DAZUJO010000000
VERSION     DAZUJO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.126"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8OL_002706"
     CDS             complement(99..1229)
                     /locus_tag="ACG8OL_002706"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS4818611.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8OL_002707"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8OL_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4818612.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8OL_002708"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8OL_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS4818613.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4540)
                     /locus_tag="ACG8OL_002709"
     CDS             complement(3485..4540)
                     /locus_tag="ACG8OL_002709"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4818614.1"
                     /translation="MQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFEELD
                     YAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQFFRK
                     VLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNESNG
                     ETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKIISK
                     THDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSFEKT
                     MSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQEEV
                     EYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8OL_002710"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8OL_002710"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818615.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8OL_002711"
     CDS             4880..5692
                     /locus_tag="ACG8OL_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818616.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8OL_002712"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8OL_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818617.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1953 a         1317 c         1013 g         2190 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caa
//
LOCUS       DAZUJO010000121         6387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000121 DAZUJO010000000
VERSION     DAZUJO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.127"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG8OL_002713"
     CDS             330..1175
                     /locus_tag="ACG8OL_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4818618.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG8OL_002714"
     CDS             1456..2676
                     /locus_tag="ACG8OL_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS4818619.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG8OL_002715"
     CDS             2673..5393
                     /locus_tag="ACG8OL_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4818620.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACG8OL_002716"
     CDS             5377..6321
                     /locus_tag="ACG8OL_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS4818621.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DAZUJO010000122         6304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000122 DAZUJO010000000
VERSION     DAZUJO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.128"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..506)
                     /locus_tag="ACG8OL_002717"
     CDS             complement(<1..506)
                     /locus_tag="ACG8OL_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS4818622.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            817..1809
                     /locus_tag="ACG8OL_002718"
     CDS             817..1809
                     /locus_tag="ACG8OL_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4818623.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1869..3290
                     /locus_tag="ACG8OL_002719"
     CDS             1869..3290
                     /locus_tag="ACG8OL_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS4818624.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            3283..4782
                     /locus_tag="ACG8OL_002720"
     CDS             3283..4782
                     /locus_tag="ACG8OL_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS4818625.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4821..6215
                     /gene="uxaC"
                     /locus_tag="ACG8OL_002721"
     CDS             4821..6215
                     /gene="uxaC"
                     /locus_tag="ACG8OL_002721"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS4818626.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         2041 a         1180 c         1285 g         1798 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      361 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      601 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccctaa ttaaatgagc
      661 attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt
      721 gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc
      781 gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatatcgct
      841 aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata
      901 aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac
      961 tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt
     1021 ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta
     1081 ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc
     1141 aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc
     1201 atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat
     1261 ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac
     1321 ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg
     1381 acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat
     1441 ctagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac
     1501 ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt
     1561 ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc
     1621 gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag
     1681 cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg
     1741 acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat
     1801 tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg
     1861 aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag
     1921 ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc
     1981 tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc
     2041 ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca
     2101 atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt
     2161 atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct
     2221 ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca gatactcccc
     2281 cacagagctt cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag
     2341 ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa
     2401 tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa
     2461 aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag
     2521 atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac
     2581 cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac
     2641 aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc
     2701 atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa
     2761 ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc
     2821 tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa
     2881 tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata
     2941 gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa
     3001 atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg
     3061 atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg
     3121 ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt
     3181 ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag
     3241 gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa
     3301 ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa
     3361 caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt
     3421 cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca
     3481 atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa
     3541 accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac
     3601 ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt
     3661 aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc
     3721 aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag
     3781 catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg
     3841 gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac
     3901 aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt
     3961 ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt
     4021 ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt
     4081 ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca
     4141 ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg
     4201 tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc
     4261 gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa
     4321 aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc
     4381 tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc
     4441 agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct
     4501 cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc
     4561 tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat
     4621 tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg
     4681 tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac
     4741 gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata
     4801 aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg
     4861 cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact
     4921 tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag
     4981 gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca
     5041 caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta
     5101 tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact
     5161 tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag
     5221 ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa
     5281 cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt
     5341 tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta
     5401 atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac
     5461 tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg
     5521 accacgcttt agacatctta cgctataaag aagcggatga aacagtatta gaagagattt
     5581 ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct tatcttacag
     5641 aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca
     5701 tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct
     5761 atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag
     5821 aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg
     5881 cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt
     5941 ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg
     6001 atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct
     6061 acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc
     6121 gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata
     6181 caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat
     6241 caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga
     6301 ccaa
//
LOCUS       DAZUJO010000123         6296 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000123 DAZUJO010000000
VERSION     DAZUJO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.129"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG8OL_002722"
     CDS             127..579
                     /locus_tag="ACG8OL_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818627.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG8OL_002723"
     CDS             593..1282
                     /locus_tag="ACG8OL_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818628.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG8OL_002724"
     CDS             1407..3758
                     /locus_tag="ACG8OL_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS4818629.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG8OL_002725"
     CDS             3838..4233
                     /locus_tag="ACG8OL_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818630.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG8OL_002726"
     CDS             4558..5175
                     /locus_tag="ACG8OL_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818631.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG8OL_002727"
     CDS             5216..5515
                     /locus_tag="ACG8OL_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS4818632.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG8OL_002728"
     CDS             5549..5716
                     /locus_tag="ACG8OL_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4818633.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..>6296)
                     /locus_tag="ACG8OL_002729"
     CDS             complement(5761..>6296)
                     /locus_tag="ACG8OL_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818634.1"
                     /translation="GLNIDKLFQEKASGKDTERPEFQKLLNYIREGDCVIVTSLDRLG
                     RDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNERE
                     KIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKE
                     NGISRPTIYKIKKANDL"
BASE COUNT         2162 a          872 c         1250 g         2012 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtcctt
//
LOCUS       DAZUJO010000124         6230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000124 DAZUJO010000000
VERSION     DAZUJO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.130"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACG8OL_002730"
     CDS             133..633
                     /locus_tag="ACG8OL_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS4818635.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACG8OL_002731"
     CDS             730..2577
                     /locus_tag="ACG8OL_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4818636.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACG8OL_002732"
     CDS             2580..3476
                     /locus_tag="ACG8OL_002732"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4818637.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACG8OL_002733"
     CDS             3526..3915
                     /locus_tag="ACG8OL_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS4818638.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="ACG8OL_002734"
     CDS             3902..>6230
                     /locus_tag="ACG8OL_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4818639.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAZUJO010000125         6174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000125 DAZUJO010000000
VERSION     DAZUJO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.131"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8OL_002735"
     CDS             725..811
                     /locus_tag="ACG8OL_002735"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4818640.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1622)
                     /locus_tag="ACG8OL_002736"
                     /pseudo
     CDS             complement(932..1622)
                     /locus_tag="ACG8OL_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1867..2781)
                     /locus_tag="ACG8OL_002737"
     CDS             complement(1867..2781)
                     /locus_tag="ACG8OL_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4818641.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2763..3143)
                     /locus_tag="ACG8OL_002738"
     CDS             complement(2763..3143)
                     /locus_tag="ACG8OL_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS4818642.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="ACG8OL_002739"
     CDS             complement(3593..3880)
                     /locus_tag="ACG8OL_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS4818643.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="ACG8OL_002740"
     CDS             complement(3900..4124)
                     /locus_tag="ACG8OL_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS4818644.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="ACG8OL_002741"
     CDS             complement(4579..4749)
                     /locus_tag="ACG8OL_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818645.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="ACG8OL_002742"
     CDS             complement(4764..5297)
                     /locus_tag="ACG8OL_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS4818646.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..>6174)
                     /locus_tag="ACG8OL_002743"
     CDS             complement(5318..>6174)
                     /locus_tag="ACG8OL_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS4818647.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa atataacccc tggcaataaa
     1501 aacctcttaa atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag
     1561 ctaagccaaa taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc
     1621 atttctattt attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat
     1681 aagggggcaa gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc
     1741 tggtttaata gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt
     1801 tctttggtat aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt
     1861 aacgttttag aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg
     1921 gtttcttcga gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg
     1981 tacttctttt gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg
     2041 ttgattgtag gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc
     2101 ctttcctagt ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt
     2161 tttcccacga ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata
     2221 cgttcgagtt tttttgtttt tcattgtttg atcaatcgca aaccgaattt cgtctttcca
     2281 aacaggttca tttttttctt ttaatttgat ttcagacatg gttcgttttt cgggtttctt
     2341 ccctcgttca gtgattgtta agtcatgttc aagacaaatc tcgtcattta ggtcataaca
     2401 tttgtttaaa aatgcttgac catgggcttg aaatttgttc ccagtttcca gattgacgga
     2461 gttaatgaca atgtggttat gaatatggtc tttgtctgta tgtgtgtaaa tcgcgatttg
     2521 gtggtcaggg gctatttttt tcgcatactc tgcccctagt tgattggctt tttcagcggt
     2581 aatttctcct ggtcgaaaag actggatcaa taagtgtgct tgtacacgat cgtcttttcc
     2641 atacagcatg cgaatttgtt tcatttcgct tttggcataa tcaatgtcac agtttatccc
     2701 tgttttgacg gttgctcgtg gttcggcgta attgatcgct cggctacaag aagtggttcg
     2761 gcttacttta actgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg
     2821 gataagcgtt gggcgtcgcc aggatcaacg tcgtgttggt gtgtgttgca ccacttggca
     2881 atttgattga tattcccgcc agctttggct aaatcgcgcg cgattgcctg agcaatgtct
     2941 ggcgccactt tcggtgtcac cagacgcgcg ttttgtgcct tggctttcac gaaagccggc
     3001 acactcattt gcaaagcttc agctgactgc ttcagctttg caaattcgga ttcgctcact
     3061 cgaaaactga tttgttttgg ctcggatcga taggtatatt ttttagaatg attgctagcc
     3121 aaatattgat tttgttcgct cataagtccc accctttcag tacgtcctac ggacgtatcg
     3181 ttcgcaatac cattgtacct aaaaaggtac aataagtaaa gctcactaaa gctcactaaa
     3241 gggcttggca agctctatat ttgctagcca aatacctacg tatttggtgc aaataaccgc
     3301 ttgtcaaagg ggcgcaagct cccccttgaa tccccctccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtgtatact accaagtaac
     4321 gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc
     4381 tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DAZUJO010000126         6068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000126 DAZUJO010000000
VERSION     DAZUJO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.132"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG8OL_002744"
     CDS             832..1566
                     /locus_tag="ACG8OL_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS4818648.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG8OL_002745"
     CDS             1582..2751
                     /locus_tag="ACG8OL_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS4818649.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8OL_002746"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8OL_002746"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS4818650.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG8OL_002747"
     CDS             3841..4767
                     /locus_tag="ACG8OL_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS4818651.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG8OL_002748"
     CDS             4786..6036
                     /locus_tag="ACG8OL_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS4818652.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          996 c         1246 g         1799 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaatttctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZUJO010000127         6065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000127 DAZUJO010000000
VERSION     DAZUJO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.133"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8OL_002749"
     CDS             complement(<1..300)
                     /locus_tag="ACG8OL_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS4818653.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8OL_002750"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8OL_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8OL_002751"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8OL_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818654.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG8OL_002752"
     CDS             1706..1942
                     /locus_tag="ACG8OL_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818655.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG8OL_002753"
     CDS             1949..3901
                     /locus_tag="ACG8OL_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4818656.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8OL_002754"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG8OL_002754"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4818657.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG8OL_002755"
     CDS             complement(4797..5489)
                     /locus_tag="ACG8OL_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS4818658.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            5884..>6065
                     /locus_tag="ACG8OL_002756"
     CDS             5884..>6065
                     /locus_tag="ACG8OL_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS4818659.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         1984 a         1056 c          965 g         2060 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacgc ggatttgtca taaattgtca tgcttctttt acctatgaaa tattttcacg
     5821 tgttaaagta ttcctcgttg aacacaacaa ataaaacttt ttaacgaata aaggagattt
     5881 ttcatgacat cacttaaaac tttacttttt ggaacaactt tggctgccgg cgctgcattc
     5941 ttcatgggaa cgactgctca tgcggacgaa gcttataccg ttcaatcagg tgatactcta
     6001 tcaacgattt ctcaaaaata cgttggtgat aactccttga tcaatgccat tgcagaatca
     6061 aattc
//
LOCUS       DAZUJO010000128         6045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000128 DAZUJO010000000
VERSION     DAZUJO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.134"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..523)
                     /gene="yajC"
                     /locus_tag="ACG8OL_002757"
     CDS             complement(143..523)
                     /gene="yajC"
                     /locus_tag="ACG8OL_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS4818660.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(620..1765)
                     /gene="tgt"
                     /locus_tag="ACG8OL_002758"
     CDS             complement(620..1765)
                     /gene="tgt"
                     /locus_tag="ACG8OL_002758"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS4818661.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1983..2681
                     /locus_tag="ACG8OL_002759"
     CDS             1983..2681
                     /locus_tag="ACG8OL_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS4818662.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2849..5488)
                     /locus_tag="ACG8OL_002760"
     CDS             complement(2849..5488)
                     /locus_tag="ACG8OL_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS4818663.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1700 a         1323 c         1107 g         1915 t
ORIGIN      
        1 gttatttatt attatttata tgacacaccc gattattcaa tattgagcaa tatatcccat
       61 gctcttatct ctttcttttt tgaataaaaa atgaggtagg acatctttct gccctacctc
      121 gtctttaaat aagctttgat acttattttt cttccttctc ttctgtctct tccagcgttt
      181 ctgttgtttc agtttttact tcaactgtct cagctggttc ttctgttgtt tcaacagtag
      241 tcgttgcatc atttgttacg acagtccctg gtttgaccgt tttgattgct gcacgatcgt
      301 actcaaggaa gatcccttcg cagtctaaaa caactgtccg tttgtcattg tcgatttctg
      361 aaacaactcc atgtaatccg ccgatcgtta cgactgaatc acctggtttc atgctatcta
      421 acaagttttg acgttcttgc tgttgtttct tttgcgaacg tgacataaaa aaccacattg
      481 ctaatagcgc gataaacatc acaagcattg gtaatgttcc catttttttc acctcactaa
      541 ttctgtatct acaaaaaaca ctttagcaga aaaatgctga taacactagt tttttactta
      601 atttttaaaa gattttcctt tagaagcttt ttgcgttttc tttattgaac ccgtattctt
      661 caaaaaaggc ttctctaaat tcaagcaaat tatcatccat gattgcttga cgtacttgtt
      721 tcattagatt caacaagaag tataaattgt ggtaagaagt caaacgaatc ccaaatgttt
      781 catcacattt gatcaaatgg cggatatagg cacgtgtata gtttttacat gtataacaat
      841 cacatttctc atcaattgga cggaaatcat gggcatattt cgcatttttc actactagac
      901 ggccttgaga agtcatacat gtgccatttc gagcgattcg agtaggcaag acacagtcaa
      961 acatgtctac tccacggata acaccatcga tcaatgaatc agcagcaccg acacccatca
     1021 agtaacgtgg tttgttttcg gggatcaacg gtgtagtgaa ttctaataca cgattcattt
     1081 ctgatttagg ttccccgaca gataacccgc cgattgagta accagggaaa tccatcccga
     1141 ctagatcttt cgcgctttga cgacgaagat cttcaaaccc tgctccttgg atgattccaa
     1201 ataatccttg acggtctgga ttagcatgag ctttcaatcc tcgttctgcc catcgagacg
     1261 tacgttctac tgattttttc acgtagtcgt aactttcatc gaatggtgga cattcgtcaa
     1321 aactcatcat gatatcagag cccaatttat tttggatgtt gattgctttt tctggtgata
     1381 aaaacatacg cgaaccgttc aagtgatttt tgaaatgcac gccttcttct tcgatattcc
     1441 gcatatcact taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc ggttgatccc
     1501 agttcataaa tttatgcagt ccgccagctt cttctactaa gtcttcacca gggcgtagcc
     1561 ataaatgata tgtattactt aaaataacgc cagcacccat ttctttcaac tcttctgggg
     1621 acattgtttt tactgtagct aatgttccta ctggcatgaa cattggtgtg gggaaagttc
     1681 cgtgtggcgt gatcaattcc cctaaacgcg cgccagtatg tttttctttc ttgattaaac
     1741 gatagcgaat ggcaggttgc gtcattcatt tccctacttt ctatataaaa tatcttgaca
     1801 actcccacca tcataatcgt ttcaagtgct ttttgcaagt tccctcggct tccttctact
     1861 ttatttaata ttcttttttt tatccttttt ttatgaataa caaaaaaaag aaaaacatta
     1921 tgacagaaat attttttgat acatggtata ttggtgttat taaagaaaag gagggaaaaa
     1981 tcatgcaatc ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa ggacgttggg
     2041 gacaagctgt tttactaaat ctaatcccta cattgattgg tattgctact attcttctta
     2101 ttatgattcc tattatcttg tttgccacca ccatatatga tggatcagcg agcgtctcag
     2161 aagcgacaac ttcaggaggc ggtagttcag gaggcggtgg cggaattatt tccagcatca
     2221 ttagcgcttt gttcatgagc ggtatttctt ggacttatct tgatatttta cgtggacaaa
     2281 aagatcagat cgaaccattc aaagatgcat ttcgtggatt caaaggtttg tttatcggcg
     2341 gagtgatcag cttagctgtg ctgatctcga tctttacaac attatggggt ttgctattag
     2401 ttattcctgg aatcattaaa agctattctt attctcaaag ctattttatt tactacgata
     2461 tcgtccaaga aacaggacaa acacctaaag tactggatac gatcactgca agtcgaaaat
     2521 tgatggatgg ttataaagga caacttttct ggttagatct ttcatttatc ggctggcata
     2581 tcttagcatt agcaactgct ggtattggtt atctatggct cagcccatac atttctgcaa
     2641 caaaagcagc attttatgaa caattgccaa aagatattta aaagagagtg tgacttgtga
     2701 ccgagggggt ggcaggagaa caccgtttat cgagaggcag taaaagaaac attcagccca
     2761 ttagcaaaaa ggaggtgtga caacattcat gtcacacctc ctttttgtat agttccagta
     2821 aaaagaaaat caacttagaa acagttaatc attcctcttc ttctgcttcc ggtaatcgat
     2881 ggaacttgat cagttcattc accgcgattg gtacgataga taaaatccct accaagaacc
     2941 agtgattcaa actgatgtgt actaggccga atagttcttg cgtaaatggc agcaaagcaa
     3001 cagccgttgt aatagctaat gccagtaacg ccatttctac caaagcttta ttggtcgcta
     3061 aattgacttt gaagatcgat tggctgctgc gtacattgaa cacatgcaga atcgatgaat
     3121 aagctaagat caagaatgca acagtttgac cgacatgctg acttgcgtct acccatgggc
     3181 tgatagtatc gacataacta cctaaataat aaccaaacaa ggttacgatg gtgaagaccc
     3241 ctgcatttat ggcaattttt tgcaataaac ctcgagcaaa gatcccttca tttttcggaa
     3301 tgggtggctg atccatgatg ccgtgttccg ctggctctct tcctaaagca aatcctggaa
     3361 gtccatcagc aactacattg acaaacagta gttgcacagc tgtaaatggt gctccccatc
     3421 ctaacagcat cgcaatcaaa acaataaaga tctccgaaat attacagctt agcaaaaagt
     3481 tgatagcttt acgaatattc tgataaaccg aacgtccttg actgacagcg tctacgatcg
     3541 tcgcaaagtt gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca tctgttcctg
     3601 tgatccccat ggcacaccca acgttacttg ctttcaacgc tggtgcatca ttcacaccat
     3661 ccccagtcat cgctacgact gcacccgtac gttgccaaga tttgacgatc cggatcttgt
     3721 cttctggcgt tacccgagca tagacagaga gagctttgac acgcgcatct aattcttcgt
     3781 ctgataattg atgcagttct gatcctgaaa gtgcttctga cggatcattc aagataccta
     3841 attctttcgc tatggcactg gcagtcacca catgatctcc agtgatcatg actgttttga
     3901 tgccagcctt tttcgctcgc ttgattgctc ctttactttc tggacgtggt ggatcgatca
     3961 ttccgatcag accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg
     4021 gttcttcgtc ataagtagca taaccaacag caatcactct cagtgcccgt ttaccaaagc
     4081 gatcattaac gatcgccgct tgttctacat cgcctgaact aaagcgcgga gcaagaacat
     4141 cgaatgctcc tttggtgatt gagatgtatt tttcgcccat ttggtgaact gttgtcatca
     4201 tttttcgttc agaatcaaaa ggaagctcgt ttactcgtgg atatttttct tctaattctg
     4261 cctttgtatg ataattctct tcaacagccc ttacgatggc tacttctgtt ggattacctt
     4321 tgataacaag gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt gcagccattt
     4381 tcaatatttc catcgcgctg ttcgtcattg aatcttcgat attcgttact tcatcttctt
     4441 tgctccatac ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc
     4501 agataacatt agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt
     4561 tcctcgccat cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt
     4621 ccggtacagc tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg agtggttctc
     4681 cttgcagttc accaatcaca aatactaaag ccgcagccgc taaagcgatc atactgattc
     4741 gtttgcctag ctgattcaat cggacctgta aaggtgtttt ttgcatagta ttttcgttca
     4801 aaagatccgc gatcttaccc atttctgtag ccatgccaat ggcagtgacg atcgctttcc
     4861 cacgacctgc agcaaccgta catcctttaa ataccatatt aagttggtca cctaatgact
     4921 catcttcttc agcaataaat gctgcatctt tttcaactgc ttcactttct ccagtcaacg
     4981 ccgattcttc tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat
     5041 caccggactc taaaacaaga atatcaccca ctaccagttg ctctgactca acagtggtca
     5101 ctttgccgtc tcgtatgaca gttgccgttt gtttattcat atcttgcaag gattccagtg
     5161 ctttttctgc gtttccttct tggacaatcg ccaataccga gttgatcaca acgatagcga
     5221 tgatcagcag tccttcaaat aaatccccat gttctgtagc aaatgcggta taaaaggaaa
     5281 tcaccgctgc tacaagaagg atgatcgttg taaaatcgga caagcttcga aagaattttt
     5341 tgatcatcga ttctttcggc gcctcatcga atttattcaa tccctgcttt tttcgccgtt
     5401 catccacttc ttgtgaagac aacccagtat ctggatctgc ttctgttacc ttctttactt
     5461 cttctattgt ctgcttgtat tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa
     5521 aaggtactta ttttactata acatcctttt tttatttact tcaaataaag ggcttatcga
     5581 ttatttgtta agaaagcgta aaatttaaag tgcttatatt tgcaaacgct accaaaggat
     5641 gctattatat agatgtaagg aaggaggata ccatcaaata gacagaagaa tttctcgata
     5701 gaacagtgta agcaaacaaa gttgttagaa gtacaggttt cacccagaca tgcgtgtaga
     5761 gtacaaacct ataaatagtg aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa
     5821 gaagtaatgc gatacgtaac aattgaataa cgtaaacgtc tgaaaattga ataggaacaa
     5881 gagatgtttc aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac atgaagaaat
     5941 tcaacaccgt gctttgcatg tatgatggaa agttacaatc atttctgttt atttgaatcc
     6001 ttccgaggta caagaaagcc gagcttatcg agagctcggc ttttt
//
LOCUS       DAZUJO010000129         6013 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000129 DAZUJO010000000
VERSION     DAZUJO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.135"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..541
                     /locus_tag="ACG8OL_002761"
     CDS             <1..541
                     /locus_tag="ACG8OL_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4818664.1"
                     /translation="VLQVFHPVVTELENIEKQILALINSMEQQSQPIICILPNSDAGS
                     EIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLG
                     TRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTL
                     NQILNDGEDIKWIQKQIAY"
     gene            514..1191
                     /locus_tag="ACG8OL_002762"
     CDS             514..1191
                     /locus_tag="ACG8OL_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS4818665.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1142..2323
                     /locus_tag="ACG8OL_002763"
     CDS             1142..2323
                     /locus_tag="ACG8OL_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS4818666.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2336..3313
                     /locus_tag="ACG8OL_002764"
     CDS             2336..3313
                     /locus_tag="ACG8OL_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4818667.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3313..4221
                     /locus_tag="ACG8OL_002765"
     CDS             3313..4221
                     /locus_tag="ACG8OL_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFS4818668.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            4222..4974
                     /locus_tag="ACG8OL_002766"
     CDS             4222..4974
                     /locus_tag="ACG8OL_002766"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS4818669.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            4979..5926
                     /locus_tag="ACG8OL_002767"
     CDS             4979..5926
                     /locus_tag="ACG8OL_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS4818670.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         2206 a          761 c         1140 g         1906 t
ORIGIN      
        1 cgttttgcaa gtatttcatc cagttgtaac cgaactagaa aatatagaaa aacaaatttt
       61 agctttgatc aattcaatgg aacaacaatc tcaaccaatt atttgcattt tacctaatag
      121 tgatgctggt agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa cggatattat
      181 ttatattacg aatttagagc cggaggaata cctgactata ttaaaagagt gcaaatttat
      241 gatcggaaat tcttcttctg ggataattga agctgcatca ttcaaaatac cagtaataaa
      301 tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg caaatctaaa
      361 tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta taaaaagctt
      421 gaaaaatgtt aagaacatct acggtgacgg caacagttct agaagaataa caaaaacatt
      481 gaatcaaata ttgaatgatg gggaagatat taagtggatt caaaaacaaa tagcttatta
      541 aaatatacta gtgatccata cattattatt ggggctggcg gacacgcaaa agtcattatt
      601 gaaaccttaa gattagaaaa taagttaata tatggatttt ttgatgataa gcaaaataaa
      661 atgcaagatc tgaaatatct tgggaaaata aacgaattga atagctgggc ggatagcaaa
      721 aaaaaaatct ataaatttat tgtagctatt ggtgactgtg tagtaagaaa aaagattata
      781 aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag tgctatagtg
      841 gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt aattaaccca
      901 gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga gcatgattgt
      961 agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg agtacatgta
     1021 ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa aattggatct
     1081 tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca ttccttagct
     1141 tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta gaattttatt
     1201 atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt ttaaagataa
     1261 ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta caaaatatac
     1321 tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac atcttgcctt
     1381 aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta cttttatagc
     1441 tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact cagaacctaa
     1501 tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg aaagaaacaa
     1561 ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg attatgaacg
     1621 aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag cggaatcgtt
     1681 aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga tctattcatt
     1741 taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta gtgataaaaa
     1801 atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac cgtattatca
     1861 acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta tcggacgtgg
     1921 acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca atcgatataa
     1981 ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg gcatggtgcc
     2041 aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc aagtagttaa
     2101 cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga aaccactaca
     2161 tttgcaacca gtatttcaag gggtaaaaat gtattaccat gataataatc ttagtgtgtc
     2221 agaaaagtta tttaatagcg gcttatgctt accttctgga agtaatatga cagatagtga
     2281 acaagaattt gttattgatg agtttaaaaa aatattcaag taggtgtgat attttatgaa
     2341 cataggaatt attggtttag gttccatggg gaaaagaaga cttagactca ttcaagaaca
     2401 ctttaatgat attttactat gtggtgttga tactaatgag gcgagacgaa aggaagttga
     2461 aaaggaattt ggaataccaa cttataatag tctaacagag gcaaaaaatt tgtttgaaat
     2521 aaattcagtt tttgtttgta catcccctat aacccatgaa tttattataa aagaggcttt
     2581 aaatttagat tgtaatatct ttacggaaat caatttatta aatgaatatt atgatgaaat
     2641 aatcgaagta gctaaaaaga aagatagatt actttatcta tcttctacat ttttgaagag
     2701 aaaagaaatt caatatatta agtctaaagt gacgcataat aaaaaaattt cttatcaata
     2761 ccatgtagga cagtatttac ccgactggca tccttgggaa gattatcgca atttttttgt
     2821 ttcaaacaaa aaaactaatg ggtgtagaga aatattcgct attgaattac cttggattat
     2881 agatacattt gggaaagtta aatcgtttac tgtaatgacc cagaaattat ccaatcttga
     2941 tattgattat tcggacagtt tcactcttat tattgagcat gaaactggta tagttggaac
     3001 tttaagtgta gatgttgttt caagagtagc taaaagaaat ttaactataa ttggtgaaaa
     3061 gattttaata gaatgggaag gtaacccgga ttctctattt tcatggaatg aagaaacttc
     3121 tagtatgaca gatattcaat tgtacgatga ggttatgcat gataataatt attcacatac
     3181 tattattgaa gatgcatatt tagaggagat taaagaattt ttcacgcttt tggaaaagaa
     3241 aatggaaccc caatatactt ttgaaaaaga taagtatatt attaacttaa ttaacgacat
     3301 tgaggtaaat taatgatgaa agtaattttt tttgggttag ggtcaattgg aaagagacat
     3361 ctaaaaaatc taataagcta tggaaaaaag aattctatta attttgatat aacagcttat
     3421 agtagcagtg gaaaaaaaga gtttgaagaa ataacgtata ttcaaaatag agacgatttg
     3481 gatacacatt atgatatagc ttttattaca aatccgactt ttaagcatct ggaaacacta
     3541 aatgtaattg ttgaaaaagt tgattacata tttttagaaa aacctgcttt ttctgagagt
     3601 attgatctta ctcaatataa ttatgattac aaaaatattt atgttgctgc tccactgagg
     3661 tataaaaata tcatggaagt tgttcgtaat agtattcaaa gtaaaaaagt atattctgta
     3721 agagctattt gttctactta tcttcctgat tggagaaaag gtactgatta tagaaataat
     3781 tattctgcct ttaaatctat ggggggagga gtagaattag actgtataca tgaattggat
     3841 tatattataa acctatttgg tttccctaaa tcagtaaaaa aacagtttgg taaatattcg
     3901 aatcttgaaa ttaacagtaa tgatttagca acatatatat tgagatacga tgataaaatt
     3961 atagaaattc atttagatta ttttggaaga gttcctacaa gaaaatttca attgtttact
     4021 ttagatgaat gtatcaatgt tgacttatta aataataaaa taagttttga agaaaacatt
     4081 gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga aggagttgaa atatttcttt
     4141 gaaaacgtag taacaggcaa agaaaactgg aatgatttat atcatgcaaa cgaagtattg
     4201 aaaatagctg aggagaaata aatggaaatt ttatttacta tttgtggtag agctgggtct
     4261 aaggggttta aaaataaaaa tttgaaatat ttggcaggaa agcccctagt ccatttcgct
     4321 ttagcaatga tagatatcta tcaacaaaaa catcctgaac ataattgcac gacttgtttg
     4381 aatacggaca gtaatgagtt agtagaattg acgactaagt taaatccgtc tgtgaaaatt
     4441 gtagaacgta aacctgaatt agctacagat actgtaggga aaattgaagt gatacgtgat
     4501 tcattcttac aactagaaag agaagataat atatttgatt taattataga tttagatttg
     4561 acttcacctt tacgtgaagt ttctaatctt gaagaattaa tagatgaaca tctaaaaaat
     4621 ttagataaag acgtctactt ttcagttgtt tctgcaagac gtaatcccta ttttaatatg
     4681 gtaaaaagaa ttgaaaatga agttactata gttaataaat caagatacac atctaggcaa
     4741 cagacacctg aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta
     4801 aaaaaaagta attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta
     4861 attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt
     4921 ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat
     4981 gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt
     5041 aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct
     5101 tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga
     5161 agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt
     5221 ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat
     5281 gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc
     5341 agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc
     5401 attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca
     5461 tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa
     5521 tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat
     5581 cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt
     5641 ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg
     5701 gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat
     5761 caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat
     5821 ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg
     5881 tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata
     5941 agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa
     6001 atctgggata gaa
//
LOCUS       DAZUJO010000130         5883 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000130 DAZUJO010000000
VERSION     DAZUJO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.137"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..1128)
                     /locus_tag="ACG8OL_002768"
     CDS             complement(34..1128)
                     /locus_tag="ACG8OL_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS4818671.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1317..2078
                     /locus_tag="ACG8OL_002769"
     CDS             1317..2078
                     /locus_tag="ACG8OL_002769"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS4818672.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACG8OL_002770"
     CDS             complement(2193..2633)
                     /gene="fabZ"
                     /locus_tag="ACG8OL_002770"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS4818673.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2853..3608
                     /gene="fabI"
                     /locus_tag="ACG8OL_002771"
     CDS             2853..3608
                     /gene="fabI"
                     /locus_tag="ACG8OL_002771"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS4818674.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3718..4770)
                     /locus_tag="ACG8OL_002772"
     CDS             complement(3718..4770)
                     /locus_tag="ACG8OL_002772"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS4818675.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            4896..5816
                     /locus_tag="ACG8OL_002773"
     CDS             4896..5816
                     /locus_tag="ACG8OL_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS4818676.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1781 a         1175 c         1059 g         1868 t
ORIGIN      
        1 ttctagcgga ttatttctgc aataactaat tatctatttt ttattttcaa ttaatttaaa
       61 gcttgaccat ggtttcaaaa agtcaaggag gaataattcg tcatcggaga ttcttcctac
      121 aacattgaca cgtccgtcgt ttttcatatc cttcaaggcg atttgtgttt ctcctttgta
      181 ttgaccgtac ccttcattgt caatcagtac atccccatga tgcatatctc gcgtattatg
      241 aggcgggaaa tctttatctt tgtagtatac acgagtcatc gttgaacgaa ggatatattc
      301 tgagcgatcc cctcgataac tatgaaggtt atcaaacaag cagattcgtt cgttttctgt
      361 aataccttct tccgtatcta ctttcaatgt tggataatcc gcgttaaatg cttctgccat
      421 ttctttcaat tcttcttcac ttgcgtacgc atttccaata gaaatatcat caatcaatcc
      481 tgtaaggatt aaatgcttca cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt
      541 gcacaagcca tcttgcgtag gccaagggcc aaactcagca gattgcgaat tgacgaatgc
      601 catcgtattc aaattatatt ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc
      661 taaaccagag tatcgatgag gataaaaatt atgagaacct aataaattat ccgtgtttgg
      721 cgaataactc atgatgttgt caacgtaatt cgtgcccgaa ctcatattga tctcgatttt
      781 gataccaaaa ggattgcgcg tcatttttgc ttcttctgct cctgtgaatc cgatatctaa
      841 gcggaccccg taggcaccca tcttatggaa aaaagataag tcatcgtaag aaatctccaa
      901 ctgttcaaat aacgctggat tgatatcaac cattacttcc atgcctaggc tattcgcgta
      961 atcaactact tctttaaatt ctgaaagaac tttttcttta tcatcattga tctgcagcaa
     1021 actggtaaaa acccgtttat acccatactt atgagctaaa tccaagtacg ctttgtcctt
     1081 ttcaaatgtt gaccgttctg gataaatcga gattcctaat tttcccatat taccatttcc
     1141 tcctgcatct tttcgttttt gtatccactt acattatagc aaacacaaag taaatggtct
     1201 acaccttttc tttttctttt aaaataaatg ataaattcca ataatttaag taggtatttt
     1261 ccctctattt tgttatcatt aaagataaca aaatagtaaa ttggaaagga acagttatga
     1321 agaaaaataa agttatttta agtattagtt tagcagcagc attgctgttt gctgctggtt
     1381 gttctgcaaa caacacgact tcttctagtt cgtctacaga aagttcaaaa agtacatcta
     1441 ctgcggaaag cacgactgcc agtagcgtcg atttgaacgc attggaatta ccacaattga
     1501 cgaaagatgt cgccgaaaat gaagatctgg tcgaaatggt gacgaataaa ggcacaatca
     1561 aaatcaaatt atttcccgaa cacgcaccaa aagcggttga aaactttatg acacatgcaa
     1621 aagacggtta ctacaacgga ctgactttcc atcgcgtcat caaagatttc atgatccaag
     1681 gtggcgatcc taaaggcgat ggcactggag gggaaagcat ttggggagaa ggatttgaaa
     1741 cagagatcaa caaccacctt tataatataa gaggtgcgtt atctatggct cgctcacaag
     1801 atcctaacag taacggcagc cagttcttta tcgttcaaaa tacagatgat atgcatgatg
     1861 gtcttttaaa agacgactat cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc
     1921 cttcactaga tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg
     1981 atgctattgc aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg
     2041 ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc
     2101 atagatgaca acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc
     2161 ttccttttgt tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg
     2221 aactcgcact cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact
     2281 gatcccttca ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt
     2341 tgacggaatt tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct
     2401 gattttagga taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc
     2461 atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt
     2521 gtcgcaacga tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga
     2581 taacgattag gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa
     2641 ctccttcttt caggcaattg ccattttcat tactttgact atcaaagtat catattccta
     2701 aatatttagc aatctttttt taatttaaga aaaaatagtt tgacaatcaa aacattttga
     2761 ctaataatta agaaatcaag aagttatttt agctattatt ctctattttt ttacgactat
     2821 caaattttta tcagaaacaa aggagagtca ttatgtcatt tctagaagga aaaaaaatcg
     2881 tcattatggg cgtcgctaac aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga
     2941 aacaaggggc agaaattatc tatacttacc aaaatgaacg aatgaaaaaa tcattagtca
     3001 aattggtcgg cgaagaagca ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg
     3061 aaaaagcaat gaatgccatc ggtgaatatg ctggggagat ccacggcctt gtccatgcag
     3121 ttgcttacgc caacaaagaa gaattgtccg gaaatgtgtc tgatatttcc agagacggct
     3181 atcaattagc tcaagatatc agcagctact ctttaattgc tgtatcaaaa tacgcacaac
     3241 cgtttcttgc accaaacagc gggatcgtta gcatgactta tctaggttca gaacgcgcaa
     3301 tcccgaatta taacatgatg ggtattgcga aagcttctct tgaagctgct atccgttatt
     3361 tggcagctga gttctcgcct aaaggaattc gggtcaatgg aatctcagct ggggcaatca
     3421 aaacattagc tgtcacaggt gttaaagact atcaaaaact gatccagtta tctgaatctc
     3481 gtacgcctga tcagcaaggt gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa
     3541 tcagcccact ttccagcgga atcatcggtg atattatcta cgttgacaaa ggtgtccatc
     3601 tttcataaac ttttgttgca ttctttaaag taaaacatat aaaaatcagg aaaaaacttt
     3661 ttaaagtctc ttcctgattt tttcttttta cgtttttttc aactcattac tttgaaatta
     3721 ttccacgact tcaatccagc cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa
     3781 ttcatcatac aaccgttttg ttactggtcc tacttccgtt tcactataaa atacatgaag
     3841 attgttattg tgataaatcc caccgattgg agaaataaca gccgctgtac cacatgcacc
     3901 agcttctgca aattgatcta gatcatcaat gaaaatatct ccttcaattg cttgcagtcc
     3961 tagtcgttct tcagctaact gcaacaagga atatttcgtg atacttggta agatagaggg
     4021 cgattgcgga gtgatgaatt ttccgtcttt tgtaattcca aagaaatttg ctgcacctac
     4081 ttcttcgatt ttcgtatgtg tggctggatc aagataaaca caatctgagt aatccagttc
     4141 ttttgcttct gttcctggaa gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc
     4201 agtcccctta ggagcagcac gatcgtaatc agatacaata aaattcgttg gttttagtcc
     4261 acctttgaaa tacgctccta ctggcgtaca gaagacgaca aataaatatt ctggagctgg
     4321 atggacacca atgttttcac cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc
     4381 tgttccatag ggcggcacaa aatcttcatt tgcttttacc acttgcttga cagcatcaac
     4441 gaacatcttt tcagggacca ttggcatcag caatcgtcta gcactttgat tcatccgtcg
     4501 actattttgt tcaggtcgaa agagattgac cttgccgtct tttcgacgat aagcttttag
     4561 accttcaaaa cattgctgac cataatgaag cgctggagag ccttcatgaa tcgtcaggta
     4621 attatcttct gtcaattctc cttcttccca ttgtccgtct ttccatttgg caataaaacg
     4681 ccaaggagtt ttgatataag aaaaacctag attgttccaa tcgatttgca tatacttacc
     4741 accttcgtta ttttctccac attttatcat tatccatatt atttgtcatt ttattttcag
     4801 cttttttcct tcattatagt tatttttgct atttcctttt ttgaaaactt cttggtacaa
     4861 tacagaaaga atcaaatttt agaaaagagg attttatgtt ttcattatta gcaactgccg
     4921 gtacgacaga ctcttctggc gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt
     4981 ttcagcggtt ttgggacggg attgattggg acgccatttt ggctgccttt atccaaaaaa
     5041 gtttatatct gatttttttg atcgtccttt tttggattct tcaccgtgtg ggaaaatatc
     5101 tgattgataa gtcttttaat cagtattcta aaaaagccat tctaaacgag agccgcttaa
     5161 agactttaca tagtctttta aatactattt ttcattatac ccttggtttc ttttttatct
     5221 atgcaatttt atctgctatc ggtgtgccga tcggatcact tttggctggt gccggaattg
     5281 caggtgtggc aatcggtctt ggtgcccaag ggttcatgag tgatgttatt accggatttt
     5341 ttattatcat ggaacaacag atggatgttg gggactacat caagctagca aacttaacga
     5401 ttgaaggaac cgtcgtatct gttggtatcc gtacccttca gctgaaagca acggatggta
     5461 ctgtccattt catcccgaat cgaaacatca ccacgatcag caatctctct cgtgcaaata
     5521 tgcaagtact tttagatatt cggattgttc ctgaagaagg atatgacaaa atctatgaga
     5581 tcattgatcg agtaaatcgt acattagctg aaaaatacca agaagagctc caaactgaac
     5641 cttctatctt tggattagtg gatcttggaa acagcaattt tgcgattcgt acggtctgct
     5701 acgcattgaa cggaaaacaa tatgccttaa aagaagagtt cttgagctcc tatgtcaaag
     5761 aattgacagc ttctggtttc acgattccga acagtccaat tgctgtcgga aaataatttg
     5821 gacaaacgat gcaacgaatc tcactaaaaa gagcgtggga caaaagtaaa gaacactttt
     5881 gtc
//
LOCUS       DAZUJO010000131         5808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000131 DAZUJO010000000
VERSION     DAZUJO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.138"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACG8OL_002774"
     CDS             complement(49..2220)
                     /gene="copB"
                     /locus_tag="ACG8OL_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS4818677.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2240..4426)
                     /locus_tag="ACG8OL_002775"
     CDS             complement(2240..4426)
                     /locus_tag="ACG8OL_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS4818678.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACG8OL_002776"
     CDS             complement(4426..4635)
                     /gene="copZ"
                     /locus_tag="ACG8OL_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS4818679.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4648..5088)
                     /locus_tag="ACG8OL_002777"
     CDS             complement(4648..5088)
                     /locus_tag="ACG8OL_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS4818680.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5162..5701)
                     /locus_tag="ACG8OL_002778"
     CDS             complement(5162..5701)
                     /locus_tag="ACG8OL_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4818681.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1503 a         1305 c         1015 g         1985 t
ORIGIN      
        1 tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt tttcattatt atctgatttt
       61 caatgtcaaa gcatttagag ctacaactac cgtacttaac gacatcaaga cagcaccaac
      121 tgctggactt aatatgatac caattggcgc aagaattccg gctgctagcg gaattgcaag
      181 aatattgtaa cctgcccccc accagaggtt ctggatcatt ttctttcgcg tttgcttggc
      241 tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt gtcaatacga catccgcaga
      301 atcgattgcg atatctgtcc ctgcaccgat tgccatcccg attgaagcac gggctaaact
      361 tggcgcatca ttgatcccat cacccaccat gattactttt ttcccttggt caaggtattt
      421 ctggacaaca gcttctttat catctggtaa taaaccgcca taatattctt cgatccctaa
      481 ataatctgct actgcttggg cagcgttttt attgtcacca gtaagcatga caggagtgat
      541 ttttctttct ttcaatgttc ggataaattc ttttgcttct gtttttacta cgtcacctaa
      601 agcaacgatt gcagctagat gattttctaa aatcaggaag ctgatggtat ttccttgttc
      661 ttggtaaggt ttcaataatg cttgttctac gtttaatccg agacgctcaa cttctttttc
      721 attaactatt ttcacttgtt gttttttgac tgttgcctct agcccgactc ctgaaagatt
      781 ttttagattt tcagcttgat aaggcttgat ttcatgttct ttcaaataat tcataatgcc
      841 aacagccaat ggatgattgg cattcgcttc caaggctcct agatattgaa ggatttcatt
      901 tttattgaac gcatcatcaa gtacttcgac acccgttaca gtaaattttc cttctgttag
      961 tgttcctgtt ttgtccagca tgatcacatc tagatcattg gcttgctcca gtgcattgcg
     1021 gtttttcaaa agtaatccgt ttttagctgc tattgatgtg gaacgagcaa caactaatgg
     1081 gatagctaaa cctagagcat gaggacaggc gatgataaag actgttacca tacgttctaa
     1141 tgcattagga agatctgtca agaacaacca ggcgatgaat gccaggatac ccacaattaa
     1201 tgcgacataa aacagccatt ttgccacttt gtcagataat gactctagct tggatttttc
     1261 tccttgtgct tttcgcacca tttccataac cttcgctaga taaccatttt caccagttcc
     1321 agttacttcg atttcgatcg ttccatcccc attgatagaa ccgccaatca ctttgtcatt
     1381 ggcttgtttt ttgattcctc ttgattcgcc agtcacagct gattcgtcaa caattgtttc
     1441 acctttcaag attacaccat ctgttggcat tttatcacca gaccggacga tcaaacgatc
     1501 accttcgttt acttctttca atgaaactgt ctcttctttt ccatctttcg tcaagcgttt
     1561 cactgattct ggcagtaatt ctgctagttt ttgtaaagca ttgctggcat tcgacacagc
     1621 attcatttca atccagtgac ctagtagcat aataacgatc aaagtagcca attcccagaa
     1681 gaaatccatg acgtgtgtat gtggattgag cagatttgcg ataaacgaat agacactata
     1741 aatatatgca actgtaatcc ccatcgcgat cagtgtcatc atagcaggac ttttttgttt
     1801 caattccatc ttagctccac ttaaaaatgg ctgaccgcca tagataaata gtatagttgc
     1861 taagatcaat acgagccaat cagaaccagg aaacgttact tgaaatggaa attccattcc
     1921 catcattggt gaaaaaagaa tgattgggat agctaggata agcgatagcc aaaatttttg
     1981 tttgaaattc cccatgtgca tggaatgatc catgccgccg tgttcgtgat ccatgtgcat
     2041 cgaatggtcc atttgtttgc tgtttgctat atctccatgg ttatgtccca tgtgcatgga
     2101 atgatccatc tgactatggt catgtttcat ctccatctgt ttttcatgag attctgtact
     2161 ttctttgttt actacttctt ttttatgctc atgttttttc tccatatcgt tcccattcat
     2221 ttttactcca tcccttctct tactttttca tgtgtcgatt taagcacagg gagttcaaca
     2281 atacactgac ggaactaaac gccatcgctc ctcctgcaat gattggattc aaaaagccta
     2341 gtgcggcaaa aggaatacca atcgtattat agacaaaagc ccagaaaaga ttttgtttga
     2401 tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc aatggatgcc aaactgctat
     2461 tcatcagcgt cacatcggct gtttccatcg caatgtccgt tccacttccc attgccatac
     2521 cgacatcagc taatgctaag gccggtgcat cattgattcc atcgcctgcc ataccgactt
     2581 ttttgccatc tttctgaagt ttttctacgt aggctgcttt atcctctgga agtacttcag
     2641 caaagatatg ctttggatca ataccaactt gtttaccaat cgtttcggct gccagtttgt
     2701 tgtctcctgt cagcataaaa acatctaatc ctttattctg gagctgcttg attgcttgtt
     2761 tgacttctag tttgatttga tcagccacgg caatcaaccc gatcacttgc ttttcatcag
     2821 ctaaaaacat gactgttttt ccctgctgtt ctaattctaa cgcgtgttct tggtattcat
     2881 caaatgatag gttcaattca attaatcgtt tacgtgtacc cgcaaaatag cgtactccgt
     2941 taatcgtacc gctgattcca gcacctgggt gggctgtaaa atcaatcata tcgtatgttg
     3001 ctgcttcttg cagttttccg tattccacga ttgctttacc tagtggatgt tcagaagctt
     3061 gttctaaagt ataaaaaata gataggactt ctttagaacc gactagatct gtaactttgg
     3121 gctttccttc tgtaatcgtt ccagttttat ccaaaacaat gctgtctaag tgagcagctg
     3181 cttctaatgc ttctccgcct ttaattaaaa ttccatttcg tgcaccaaca cccgttccta
     3241 ccataatagc tgttggagtc gctaaaccta atgcacatgg acacgcaatg actaatacag
     3301 agacactatg gatcaatgcg agttgccaat caccagtgat caaacctgta gctatgagcg
     3361 tgataaacgc gatgactaat acgatcggaa caaaaatccc tgagatccga tctgcgattt
     3421 tttggattgg ggctttgctc ccttgagcat cttccaccat ttgaatgatt tgcgctaata
     3481 ctgtctgctt gccgacttgt gtaacttcca tgtgaagtag accattcgta ttgattgtcc
     3541 cgccaaagag cgtgtcatcc ggattctttt ctacaggaag attttcacca gtaagcatac
     3601 tttcatctat cgcactgctt cctgaaataa ttcgaccatc tgctggaatc tgctcaccag
     3661 gacggatgac tagttgatca ccaacgacta cttcttcaat cgctaacgtt tcttctttcc
     3721 cgtttcttat cacttgggcg gtttttgttt gaagagacat caattgtttg attgcattac
     3781 ctgttttcgt ttttgctgta tgttccaaat atttgccgag caaaatcagc gtgatgatca
     3841 tactgctgct ttcaaaataa agatcttggg ggtgtccacg gaaaaatccg ttgtaaatgc
     3901 tcaatgcaaa ggcagccgat gttccaattg ctaccaatac atccatattt ggtgctttcg
     3961 tcttgattgc atggtacgca cctttataaa aacgagcacc gacataaaat tggacaggag
     4021 cagacagcac taactggacg attggaaaat gaaaaaagtg aacaatcgcc gcatggcttc
     4081 ctaacatcat agcaatcatt gacaacatca acggcaaagt caaaaccgta ctcaatataa
     4141 gatcgaatag catttttttc aaatacgctt gcttttcttc tgcaattttt tgtttgtggg
     4201 cttcatcgta caaaattgca ccatagccga tgttttctac gctttggatt aatttttcag
     4261 tggtcgtccc ttcatatttg acacttgctt tttcagtggc caagttgact gtggcattga
     4321 ccacaccagg ttgtccgttc agctcttttt cgatcctagc agaacaattt gcacaagtca
     4381 tacctgtaat gacgaacgta tccattttgg tttctgctgt tgccatcaaa tcacctctgc
     4441 tttataaccc aatccattga ttgcttgaca aatcttttcg gattgaactt ttgtttcatc
     4501 aaacttgacc gtaccgtttg cttttttcaa atttactttc actttttgca cgccgtcaag
     4561 tgcgcttact gtttcttcta ctcttgctgc acaatgattg cagctcatac cttcgattga
     4621 aaattgttgt ttcatttttt cttcctccta ttgatgttgt ttacattcac attgacctgg
     4681 gatacagtta cattcgatcg tatctactgg ttgcttttgt tccagttgtt tcaaaatctc
     4741 attgatatct tcttgagtca aagtggcttc ttctactaga tccgcaatcg tctctccaat
     4801 cctttttgca cagatatggg aaaaaagatt ttccgtcgca ctgcgaactg tttccatctc
     4861 cgagacagct ggataataaa tgaatttttt tccttcttgt tctgtccaga tcacttcttt
     4921 ttttaccaaa cgacccagca aagttttgat cgttgctggt ttccaatcca tcgaatcagc
     4981 taagatctgc gtgatttctt gggctgtcgt ctgtccaagt gtccaaataa cacgcatgat
     5041 ctcccactct gaatcactga tctttactgg cgttttgact ttttccattt atttcacacc
     5101 tttcgtttac aaatgtaatc attatcttat gaatacagat tacacttgta aacaaaaact
     5161 gtcaattatt tttgctctct attttcaatt tagttaaaaa ttcttgtttc tctcgtcttg
     5221 ttttaaggat gatcagttta tttcttgcat tagctacttg gatcagctgc tgtattttcg
     5281 gttcattgtt ttcttcaaaa tgccagataa aagataaaaa ttctaagtat tccctgttta
     5341 gtttctccga aaatgattca ggcatatctg gccgagattt tttgtttatc tttcttttta
     5401 gagaacgcca aactactcgg tagatggcga gataacgcgg cactttaagc caaatgatct
     5461 tatctgcttc tttaatcctt tcctctaatg tagaagtata atttccttca acgatccatg
     5521 acggattaga taccataaac aattgctgtt cttgcaaaaa ccactttttt gcctgaatac
     5581 tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag cggaaagtcg gtgatttctt
     5641 tgatcttcct tgcgagggtc gattttcccg acccactttg tccaatcacg atgatcttca
     5701 ttcttctccc tcacttctct ttggaatttg gttcgatcat accacttata acgccgcaaa
     5761 aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaa
//
LOCUS       DAZUJO010000132         5551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000132 DAZUJO010000000
VERSION     DAZUJO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.139"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8OL_002779"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8OL_002779"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFS4818682.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8OL_002780"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8OL_002780"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFS4818683.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8OL_002781"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8OL_002781"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFS4818684.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACG8OL_002782"
     CDS             complement(4028..5044)
                     /locus_tag="ACG8OL_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4818685.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5298)
                     /locus_tag="ACG8OL_002783"
     CDS             complement(5146..5298)
                     /locus_tag="ACG8OL_002783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818686.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
     gene            complement(5315..>5551)
                     /locus_tag="ACG8OL_002784"
     CDS             complement(5315..>5551)
                     /locus_tag="ACG8OL_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFS4818687.1"
                     /translation="CSCCGEYYEFVGMSVRKNGRLVIKYAKDNHAKRFMREENRKIDQ
                     KEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
BASE COUNT         1461 a         1377 c          973 g         1740 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac a
//
LOCUS       DAZUJO010000133         5366 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000133 DAZUJO010000000
VERSION     DAZUJO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.140"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..1213
                     /locus_tag="ACG8OL_002785"
     CDS             254..1213
                     /locus_tag="ACG8OL_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4818688.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKKGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(1300..2310)
                     /locus_tag="ACG8OL_002786"
     CDS             complement(1300..2310)
                     /locus_tag="ACG8OL_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4818689.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNGGKQFRVYTDGRISQRIGMTNNFQM
                     IK"
     gene            complement(2392..3282)
                     /locus_tag="ACG8OL_002787"
     CDS             complement(2392..3282)
                     /locus_tag="ACG8OL_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFS4818690.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            complement(3275..3571)
                     /locus_tag="ACG8OL_002788"
     CDS             complement(3275..3571)
                     /locus_tag="ACG8OL_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818691.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            complement(3727..4272)
                     /locus_tag="ACG8OL_002789"
     CDS             complement(3727..4272)
                     /locus_tag="ACG8OL_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS4818692.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            complement(4446..4745)
                     /locus_tag="ACG8OL_002790"
     CDS             complement(4446..4745)
                     /locus_tag="ACG8OL_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS4818693.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            complement(4772..5080)
                     /locus_tag="ACG8OL_002791"
     CDS             complement(4772..5080)
                     /locus_tag="ACG8OL_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS4818694.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
BASE COUNT         1640 a          996 c          795 g         1935 t
ORIGIN      
        1 ttctataaca ttagaagaaa ttttaaatac ctttgataaa aaatattgat gttttttctc
       61 tcattgttta caaactagat agcttatgta ttcttattaa aaataataga tatttcgaac
      121 atcatcactc tcatctactt aatttcgcat ttttacttta cgccgattgt aaaattaagc
      181 tagacaacta aaaaagtcat ttgtgctaca ctcaaaatag ttccgaccaa agaattataa
      241 ggagtgagta caaatgacct atacccatct tacaacggat gaacttgtaa taatagagtc
      301 ttatttcaaa attaatcaat ctgttgctaa aactgcccat tgcttgaatc gttcaagaca
      361 aacgatccat aaagtatatc tgttcttcaa gaaaggaaaa tcagccttag aatattatca
      421 acagtataag aaaaacaaat caaactgtgg tagacgtccg cttgttttac ccgaggaaca
      481 atcagaatat attcaaagaa aggttgttca aggatggaca cccgatgtga ttgttggtcg
      541 tgcagcgttt cctattagtt gttctgctcg taccatttat cgtatgttca aaaaggggct
      601 atttgattct tctgacttac cgatgaaagg caagcgtaaa ccgaatggac atcaagaaag
      661 acgtggaaaa caaactttcc gccgctctat tcatgaacgt gaaaaggatt atagccaatt
      721 ctcaaatgag tttggtcacc ttgaaggtga cactatcgta ggtctgaaac ataaaagtgc
      781 tgtaattacc ttagttgaac gattatcaaa agttatcatc acattgaaac cgtgtggtag
      841 acaagcgatt gatattgaaa aaaaattaaa tcaatggttt gaatctgtac cgaaaaacct
      901 attcaaatcc atcacttttg attgtggaaa ggaattttca aattggaaac agatcagtaa
      961 tgtcaatgat attgccattt atttcgctga tccaggaacg ccgtctcaaa gaggcctaaa
     1021 cgagaattct aacggattgt tacgtagaga tggtttattg aaatctatgg atttcaattc
     1081 agtagatgaa ttttttattc aatctgtcgc atctaaacga aataatattc ctagaaaatc
     1141 actggattat cgaacacctt tggaagtatt tttgagttac gtaagtattg atgatctgtc
     1201 taacttaatt tgacaattaa gattataaaa aaactgattc taagaaaatt aaataatttt
     1261 tctcaaaatc agtttttctt tgataaataa tatttttaat tatttaatca tttgaaaatt
     1321 gttagtcatt ccaatacgtt gagaaattct tccatctgta taaacacgga attgttttcc
     1381 tccattcatt accgtatttc caatatcatc agtagaaata tcaactggat aaaattctcc
     1441 attttcatct tgcattaaat cttttctata ttgggtggat tcaataatat tagtatttgc
     1501 ttcattttta tctggagact cataaatcca taacgcttta ttatttagta cagtttcttt
     1561 agcagcttcc caatttgaaa ttcctgaact acttgtagat tgtgtcgttg tagttgttgc
     1621 ttgttgagta gattcttcta ctactggtgt ttggggagtt tgtccattaa tgatatctgt
     1681 aaatccttgt tttagctgtt gattttgagt ttcgttaaag tacagataat tattttcatt
     1741 accttgattt tgttgcgtta aataaacttt aggttgtcca ttttgaaaac cgaaaaagta
     1801 aacatgctgc cctaaatatt ctccagtttc tacatcagaa taaactgcta ctaaattaaa
     1861 tcctgcattt ccagttccat cttctgacca ttgtacagta actggtgctt cattgattgc
     1921 cattttccaa tttccatcaa taactgcact tggtaaaggc gtaccataca gacttacatt
     1981 gttctgagga ttgtagcttt tatattcttg acctaatgtc tttccccatt gtgggacaaa
     2041 agttgctaat tgagatgctt tatttgtatc ccataattct tgttttattt gaaaatcact
     2101 cgtatctgaa ctcgttgcct tactagaaga ctctgttgtt ttagtttctt gagtagttgt
     2161 ttctatttct tttgtagaag attcctttct tgttgtttca gtttcataag ttgttgaatc
     2221 cacttgtttc ttttgttcat tttgagagac agtagaacat cctccaagta aaagaatcgt
     2281 gcctaatagg aataatcgtt ttgctttcat cctttttctt ctcctaataa taaatttgaa
     2341 ttcattcaag aaaaagaacc acccgtataa gtggttgctt tttctaaata attatttctt
     2401 taacagctct tgcttcaatt gttgaaactc ttcttctgta atcatattcg tttccaacaa
     2461 gtgagaaagt ctttctaatt gagaaagaac tgtatcagaa gacacgatat cagaagatac
     2521 ttcttcaaat tcaccttcta aaatatctaa atcttgttgg agattttttt ctgtatacgc
     2581 tacatttttc cctaactctg tttgaagctt cgttgccatt ggtttcatag acaagtaatt
     2641 gatgcctcct gaaacaacgc ctcccacaac tggtactgct ttagaaatac ctttcgcaaa
     2701 ggtattttta gttaactttg taccaaaaat acctagtact cgtttgatta ctggataata
     2761 cagcgtttgt gtcaatgctt tttgaggaat ctttttcaat gcttgtgtag acagctttcc
     2821 agacatcgct cgtacggcag aacctgcaga agttactcca agcataattc ccaaatacaa
     2881 tatcagcgta ttctttgctt cttcactgaa ctctccagtc tctcccatta aatccttaaa
     2941 tccataaata tacgaaattt cctgtgctaa tcgtaatgaa taaccaaaga actgaaggag
     3001 atccgctgga atagttgctg ccatagcgat acctcctggc agtccagttg ctaacgatac
     3061 cgcacttgat ttcaaggtaa tcgattgaat acatttttgt gcttcacgat ctagcatttc
     3121 tttggataaa aatgcttgtg gacccttttc tatgagtcct tcaatatcag aaacattccc
     3181 ttgaaatgtt tgaatcaaaa atgctcgacg atcaactttg accattggta atttgaatgc
     3241 ttcttctatg attctcaaac ctactgtttc ttcattagcc atgtacatgt tgctcctttt
     3301 gttgttgtct gataatactt tgaatccatc tcgttgaaaa accataccga tccgttaatt
     3361 tcctagaatc aatggaatgg ttttcttgta tcattgtttc aattttttga gcgactaatt
     3421 ctcggtcata cattttcatt ggaaacgaca actgttgccc acgaaaagcg atataaaact
     3481 gataaagtaa atccgtgtct agtccttttt cctcaaaaaa ttcatagata cttttatacg
     3541 aactcgtcaa acattcccca tctactttca cttgctcacc tccatttccc ctgacatttt
     3601 aaaaatatca tattgattcc ttcattgata atgcatataa caagaggttt tacaagttgt
     3661 ttagtctgca aaagaacaag taaaataaca gaaaaagaaa catatacatt gaatacttat
     3721 tttcgtttat tgaaataaca aagaatgatc cgacaattgc cattcattac gaacatgaat
     3781 acttaatggg tgagataact tgccttctgc atttatcaat actttggtca tcttcttttg
     3841 tttcgcactc aattgatcgt aaatggtctt tgcttgttca taagccactt gactcgttgc
     3901 cgtaatcgaa ccacaagcaa aaataatagt gttgattttc ttttctgtaa aaatggtcgt
     3961 taatatttcc aaagattgtt catcattgcc acgagaaaat acccgaggac ttttcgcttt
     4021 tgtatacgaa aaaagattca cggcaataaa accagtaaat cccaattgtc ggatattctt
     4081 ttcgattaat aacatactca aatcattcgt aaatggttct gtatctgttg gacgaatggt
     4141 aatgactact gctaattttt ctttctcttc ttttcctttg attgcccatt gtcgtttgaa
     4201 aacatagcga tgggtatgat gaggatctcg ccaaacttct gtttgtgtgg tcactaattc
     4261 tttttcaatc atctcttttt ctcctatgct aatttttgtt caagcaattc aattaccatt
     4321 gccgttataa gtgtcaatac tagttgaaaa tatttcatat tctttctcct tttttcttca
     4381 gaattcgata aatcgcggta aggatatctt ctaaacattt cgcaatcatt ctcaaataat
     4441 cgatcttatt cattcgttcc tccaatctga ctccaaacca atcgtccttc taccagtaac
     4501 ccacaaacaa attctcgctg gctggtaata tctgtaatga aataaactac atagtctttt
     4561 ccatcttctg gtgcgcaata aatataaaga tgtgtcatca ctaaacgtgg tagatgaaca
     4621 tttttaggaa gcgtacccat ttcaatttcc tctttttcca tcgtaacgga ttcatagccc
     4681 cactcttcag gagtagtgac tagctgtgca cacttttcca gtacttcgat agccgtttct
     4741 aacaagtcat ttcgctcctt tcttttagat atcagtattc ttctggtaat aacatcgttt
     4801 gatactcacc cgaatcaatg acccatattt tttgagacac tgattccaaa cgaagcttag
     4861 gtgaaatcag atactctttt cttgcttccg gttcttcttg acgatgaacc aacttttgat
     4921 ttcctttttc tatcgaaaat tcaaagattt gaagatagtc taaaggaagc ttcttttgaa
     4981 cgttttgatc gattaaattc caacataaag tttgtaattc caccagtaaa ctgtccatta
     5041 ctccctttgt gacataacgt tgtcctttat tttgaaacat ttcttttccc tcctttgtcg
     5101 taaaaaatag cccaatcttc tataaagaag attggacttc atggatataa tatatatttg
     5161 aatttatctc tatttcattg ctatattatt tatttcaatt aactgaatag acatcacaac
     5221 taaatataag aaatgctttt ctaaaagtat ggagttttca tatttagtac ctctatacta
     5281 aattgcgact acaagtttaa aatttatata gttattagta actaagatac ttaattttgc
     5341 ttaatgaact atcctattaa tgttat
//
LOCUS       DAZUJO010000134         5317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000134 DAZUJO010000000
VERSION     DAZUJO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.141"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(405..779)
                     /locus_tag="ACG8OL_002792"
     CDS             complement(405..779)
                     /locus_tag="ACG8OL_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS4818695.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(802..2202)
                     /locus_tag="ACG8OL_002793"
     CDS             complement(802..2202)
                     /locus_tag="ACG8OL_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFS4818696.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2206..2616)
                     /locus_tag="ACG8OL_002794"
     CDS             complement(2206..2616)
                     /locus_tag="ACG8OL_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS4818697.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2634..3125)
                     /locus_tag="ACG8OL_002795"
     CDS             complement(2634..3125)
                     /locus_tag="ACG8OL_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS4818698.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3135..3926)
                     /locus_tag="ACG8OL_002796"
     CDS             complement(3135..3926)
                     /locus_tag="ACG8OL_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS4818699.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3919..4692)
                     /locus_tag="ACG8OL_002797"
     CDS             complement(3919..4692)
                     /locus_tag="ACG8OL_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS4818700.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4848..>5317)
                     /locus_tag="ACG8OL_002798"
     CDS             complement(4848..>5317)
                     /locus_tag="ACG8OL_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4818701.1"
                     /translation="YIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFT
                     IEGASTKLRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGD
                     GITNEHLKEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         1701 a         1067 c          697 g         1852 t
ORIGIN      
        1 taggagtgag catttttcga aaaatactaa aaatttttca aaataaaaaa aggcatacag
       61 acaataaact gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct
      121 gcaaaaaata tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact
      181 ttgcttggta agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat
      241 tccaataaat aggaaggatt acaaaatttg aaaccaaata agtaaaaaat actgtcactt
      301 tgaattaatt tgaaatagta aattctcatt aaactattga aatgctgata ccaaaaaatg
      361 aagtactttt cgttcatacc aacttagagc acttcgttat ctatttattt cttcatttgg
      421 cttatctcta taatctcatg atttggtccg tagaaattaa agtaacggat tccattttcc
      481 cagaacggta attccataat atttttatct aataattcat atcctaaatc ttgaattgaa
      541 ttgaatacct catctatgtc atccgtatct aatgctagat gatcaatggc tcctctacac
      601 tcagtagttt tatcttccca tatttctatt atcgttccat tcaactcgaa aaagccgact
      661 ttacttccca cattcatttc ttcattaatc agactaaacc ctaaggattc ataaaagatt
      721 tttgaagctc caaaatcttt tgttggaatt ccaatatgtt gtaaaccatt taatttcact
      781 tacatcatcc cttcaattgt tttaaaatcc acattcataa tttattgaat agtctagttt
      841 atctaccttg attgctccct ccgtaacagt catacttaat gctgttccga ctttatcata
      901 cattctagta cttaaagcaa cattatcaat atataaaaca gctatcgtat catcaacaat
      961 taactttagt tcatattctt tgcctatttc caaaaatagt ggtcgttgca gccccttatc
     1021 aaacatttga ggccaaggat agttcggagt ttttccgaaa gtaagctgat tattaatcaa
     1081 tgaaatacga aattcgtatc cttcttgagt atcactatct acaaataatt ttaaagcaaa
     1141 ctcctttgtt aacgatgtaa tagtcaaagt tgtttcaaat agaaaataat cttcgatttt
     1201 aggaacaatt actctttctt tttttccaaa ttctttctct aacttaaaac ttgaaaatgg
     1261 agtgatttct ttaaaattct caattatttt ttcaggcagt ttaacaccaa gagttttgtc
     1321 tgtcttttga ataatttgat gtggaacaag tgcaccgccc caatcccagt tattttgatc
     1381 gttctcccct tcttttgttg ccacccagcc agttagaaaa cggccagttt cattagttcc
     1441 gactgttctt gctgcataat aggcttttcc gtcgaaagct tcgtctactg gtgatttcca
     1501 atctgaaaat aatcctgctc cgactcgata tttagtcttt ttatcttcac aatactctga
     1561 aaaaagtaaa taccacgttc catcaatttc aaatatatca ggcatttcaa tcatgttaaa
     1621 ttcattatta gcccagtagt ctcctttgaa attccaatct tttaaatctt cggattcaaa
     1681 atagaccaat ctccccgtcg gctttttctt acttgctggt tttctagctc ctagtatcaa
     1741 aacgtatttt ccatgatctt gatcaaaaat tgcaaaagga tcgcgccaat cattcttatc
     1801 atatccttct tgaggaacaa gttttgctgc ttcgccatcc tttttccaat gaattaaatc
     1861 tttagaactt gcaatcatta acacttctga agtttttcca tccaacttag ctttgcgatt
     1921 atgtccagta tacaaagcta aatactcatt gattccttgg acaatacttc cagcgtaaat
     1981 gtattgatca atgtcatcgt tgccaccacg atgaattact tccccgtatt cttcaaattt
     2041 cacaaaatca gtggttgtaa ctagtgacca cccaaaagga tcagttaaag gtttaggatt
     2101 tctcgtatct cgttgatgaa atagataaaa agttccatct ttataaaaag gcatgcaatc
     2161 cccaaaccaa aaatctttag gcttgtaaaa aattttttgc atttatcact catcctcttc
     2221 gttagtatgt ctttgcatat ctgcaagaca cgcaattgta ttcatatttc ttagattttc
     2281 gatgatatca tcgctttttt ttccttgtaa tctcaaattg acagcctcaa tcaaaattcc
     2341 taaattcatg ccaccaagaa gttgaaaccc attttgaaat acttcacttc ttttagcaaa
     2401 ttcgttgaaa ggtgttccac caatcaagtc agcaaatacc ataacttctt gattttttac
     2461 ttgagaaact attttttcaa tgttttcacg gaattcttca atgcctgcat ctccaagtgt
     2521 taccacatga caatttaaat tatcgccaaa aaacattttt aatgtatcta caaatccaat
     2581 ggcaatattt ccatgagatg taattataaa ctccatccac tcatcctctg ttcttaataa
     2641 ataaaatcct ttaaaaattc ttttgaatct ctaggagttt gtatcactgt cacttcaact
     2701 ccatcactaa ttagttcttt aaaagcctct ctctcttctt cagtaatatt tatcgtcgga
     2761 cggatgcgtt ctgttccatt ccttcttgat aaatttccta cgtttacact tgttattggt
     2821 aaaccgttat caatcaaaaa tttaacatcg attggagaaa ctgcaacaat aagaactttt
     2881 tgtccttcat atttaccaga aagaatattt ttaactgcta cttcccttgt aattattgaa
     2941 gttctaacat taggtggtgc agcgaggcga actacagctt tttttacgtc atcattggct
     3001 acttcattat taataaccat aattctgtta gcactcaatt gatttgtcca ttgtgttgct
     3061 acttgtccat gaatcattcg atcatcaata cgaacatgta taataccttt cattttttca
     3121 tccatattca acacctactt caaaatattt aacgctctta acgcaataga aactactaag
     3181 attaatagaa ttacataaac tggtttaacc tttcgtccta acaaataata cacaattgct
     3241 actacacatg caggtaataa accaggcata acttgatcaa agatttcatt tccggtcatt
     3301 gttaagtcgc cttgtttaaa ttcaaagcca acattcactt ttacaactgt ggcaatgagt
     3361 gcaccgacca ccattaaccc taaaattgat actgcatcag taatagactt taattttgtt
     3421 tcaatttgag ttagtaggct cgcacccgat tctttcccag caaggtataa aggataagca
     3481 gtaatcctta ataaaattga tgccagtatc cacaaaataa ttccaacaaa actaccttcc
     3541 aatgccatat tagctgcaat tgctccaaaa atagtccatg gaataactac aaatacacta
     3601 tctccaattc ccgctaaagg acccattaga cctgttttca gagcacttac tgcttccaaa
     3661 gacttatttt tttcttcttt ttcaattcca acattcatac caataatgta aggcgctgta
     3721 tacggcgtag tgttaaaaaa ttgtaagtga gctaaaacgg attgtttcat tccttcagga
     3781 tcatctttat atgtgtcctt gataaaagga agcatagaat acgcataacc caacccttcc
     3841 attttttcat aatttaatga agttgcacca ttgagaatcc accggaaaat tacttgtttg
     3901 tcgatttttg tcagtttctt attcatcttc gtcaaaacct cctgttgcag ctggtgctac
     3961 ttgagtatta tttgatctat ttttatatac gattagtgcg attgcagcgc caagtaacga
     4021 aatccctaaa attggtattt ggaaataaac tgccaaaacg aaaccaacta acaaataggc
     4081 aaagtaatct tttgtaggta aatatttaag tagcattcct atcccaacag caggtaatag
     4141 tccccctgct acctttaaac caccggtaac ccattcagga atataattga ttgcacttcc
     4201 cactaacgtt gggcctaata tcacagttaa caaaacagga attccagata caaagcttgt
     4261 attaattgat gccaaatatt gcatccactc taatttttga taatcttctt tttgtgcgaa
     4321 tttttctgcc ttttgttgaa aaaaaatgtt tgttgaccac tttagaacat ctagttgtac
     4381 aattaacatt gctaccggta ttgctaaagt tattcctata gaagcatcct ggttagttga
     4441 gattgctaaa taagtaccga tcaatgctgc tgtttgataa tccggtactg atgcaccacc
     4501 aaaactagta atacctagtg tcattaattg caaagtccca ccgatataaa gtccagtttg
     4561 aatatctccc ataataagtc cggtcaagaa acctgcaacg acaggttggg aggttccgat
     4621 tttgggccct tccttatccc agttaatgaa aaatccatac aacacaacta aaaatgtttg
     4681 ccaaaacgcc attttgttct ctcctttctt cttatacttt tatataagca attactgtgc
     4741 caactaacat tatccaataa agcgggaaaa tacagtaaat attaattaaa tgagcgtcta
     4801 aatatacttt ggacaccctt tcattttttt agacgctagt atgctattta gaaaatagtc
     4861 tgctcaaaaa ttgtatagat taactcatac tctcctttgg gtatttcaat acagtaaata
     4921 tctttgattt ctgaaaaaaa atcttttgat acatttaaaa aagtatcaaa tagttgcgct
     4981 ccctctttca aatgttcatt tgttattcca tcaccaatca taattcgttc aatcattcca
     5041 gaaagatgta gaaatagatt aattctcaga aaacttggga ttttgacatc gtaatatttt
     5101 tcatacaaat atagtacttt ttctatctca ttgaatactt ttttggaatc taaaaagcga
     5161 agcttagtac tagcgccttc aatagtaaag agtttaacta attcatttaa gcagaactca
     5221 cagctatccg agtccatata cttatttaat ttttctaagg tttgattccc attaattaga
     5281 ttttctaaat ttaaacaatc gattccttga atgtaaa
//
LOCUS       DAZUJO010000135         5312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000135 DAZUJO010000000
VERSION     DAZUJO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.142"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8OL_002799"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG8OL_002799"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS4818702.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG8OL_002800"
     CDS             complement(1297..2133)
                     /locus_tag="ACG8OL_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818703.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG8OL_002801"
     CDS             complement(2317..3534)
                     /locus_tag="ACG8OL_002801"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS4818704.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG8OL_002802"
     CDS             complement(3683..3844)
                     /locus_tag="ACG8OL_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818705.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG8OL_002803"
     tRNA            3917..4000
                     /locus_tag="ACG8OL_002803"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACG8OL_002804"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACG8OL_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAZUJO010000136         5207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000136 DAZUJO010000000
VERSION     DAZUJO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.143"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACG8OL_002805"
     CDS             <1..1368
                     /locus_tag="ACG8OL_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818706.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..2989
                     /locus_tag="ACG8OL_002806"
     CDS             1355..2989
                     /locus_tag="ACG8OL_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS4818707.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="ACG8OL_002807"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="ACG8OL_002807"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS4818708.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4077..5066
                     /locus_tag="ACG8OL_002808"
     CDS             4077..5066
                     /locus_tag="ACG8OL_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS4818709.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttt
//
LOCUS       DAZUJO010000137         5201 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000137 DAZUJO010000000
VERSION     DAZUJO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.144"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG8OL_002809"
     CDS             25..696
                     /locus_tag="ACG8OL_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS4818710.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG8OL_002810"
     CDS             755..1195
                     /locus_tag="ACG8OL_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818711.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG8OL_002811"
     CDS             1207..1875
                     /locus_tag="ACG8OL_002811"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS4818712.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACG8OL_002812"
     CDS             1939..2562
                     /locus_tag="ACG8OL_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS4818713.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACG8OL_002813"
     CDS             2618..3004
                     /locus_tag="ACG8OL_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS4818714.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACG8OL_002814"
     CDS             3217..4608
                     /locus_tag="ACG8OL_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS4818715.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1729 a          861 c         1095 g         1516 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga t
//
LOCUS       DAZUJO010000138         5143 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000138 DAZUJO010000000
VERSION     DAZUJO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.145"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..1824)
                     /locus_tag="ACG8OL_002815"
     CDS             complement(58..1824)
                     /locus_tag="ACG8OL_002815"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818716.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1784..3538)
                     /locus_tag="ACG8OL_002816"
     CDS             complement(1784..3538)
                     /locus_tag="ACG8OL_002816"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818717.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3648..4217)
                     /locus_tag="ACG8OL_002817"
     CDS             complement(3648..4217)
                     /locus_tag="ACG8OL_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS4818718.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4235..>5143)
                     /locus_tag="ACG8OL_002818"
     CDS             complement(4235..>5143)
                     /locus_tag="ACG8OL_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS4818719.1"
                     /translation="SNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLF
                     VKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKD
                     INFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDY
                     QFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASL
                     SKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKN
                     GGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1713 a          964 c          691 g         1775 t
ORIGIN      
        1 tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca
       61 tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag
      121 atttttgtat atttctgacc ttcgatacaa ctcactatga gtaccttgat caactatttt
      181 tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat
      241 aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt
      301 ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa
      361 agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc
      421 gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg
      481 agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa
      541 aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga
      601 agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc
      661 atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa
      721 cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt
      781 atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga
      841 tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata
      901 aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc
      961 tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc
     1021 tactagaatg actactgcta aactaacata tgagaaaata gaggataagg ccatgactga
     1081 aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag
     1141 tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca attctatagt
     1201 ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt
     1261 ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc
     1321 taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc
     1381 cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga
     1441 catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt
     1501 tgacaaatcg tctgcaattt gcattcttaa atgcgcactt tctttataag tactattata
     1561 taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa cgaacgatac
     1621 tgtaaaataa agccaaggcg gctttacatt ttctaaaact aattcgtcta tcagcatcat
     1681 tataatgaat gctggtacca tattaatgca gtatacaaaa aagctagaaa tagatgcttt
     1741 aaccaaatct tctgcgccct gattagttaa agcgaatttg tttttataaa aacttttcat
     1801 tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat
     1861 gtttagccat taattcatta tgatttccac gttctactat tttcccttct gacaagacaa
     1921 gaatttcatc tacagattta atgctggata atcgatgagc tatcataata actgtttttt
     1981 ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg
     2041 cactggcttc atccataata acaatctttg catcttttaa aattgctctg gcaatagcta
     2101 tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc
     2161 tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt
     2221 tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata
     2281 gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat
     2341 ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta
     2401 gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt
     2461 gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat
     2521 tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt
     2581 gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt
     2641 gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca
     2701 acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta
     2761 taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg
     2821 ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca
     2881 aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt cctcggatat
     2941 attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg
     3001 tattctctgt cattttcttt aacaagaata caccaattac taacatggcg attagaacca
     3061 atccaatttt aaaattaatg aaaaagccta aggcaactga taaaacaggt gttactatgg
     3121 cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat
     3181 caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg
     3241 ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag
     3301 aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa
     3361 ccgcataact cttagctaaa tattcatctc ctacaataat tagagactta agaaatttgt
     3421 aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta
     3481 accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt
     3541 tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta
     3601 atagcgtaaa ttagagatgt acaaacagat agaaggtaaa ctacgttcta ttttttgtat
     3661 ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac
     3721 actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca
     3781 gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc
     3841 cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct
     3901 cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg
     3961 ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg
     4021 taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca
     4081 gataataatc caaaaatccc tggggatatg ctgtgaccaa acgccccacc aatactacta
     4141 accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc aataaaaata
     4201 taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca ttaaaaatct
     4261 gttctatcaa agttgaatct tctactctaa gttttatatc tttttttatt ccaccatttt
     4321 ttaaataaat aattctattg catgttttat aaagaaattc cggatcatga gtggctataa
     4381 ctactgcacc cttttctttt aagtctttaa taaggtcagc aatcctttcc atattaatat
     4441 aatccaaccc acttgtaggt tcatcaaata gaaataaatt agtttttgac aatgaagcta
     4501 tagaaaccaa taatctttgt ttttctccac cggataaatc aaacggtaat tgatctttca
     4561 ttttataaag ttccattttt tttaaaattt tttcttgctg atctttacta actatttcat
     4621 tccccagggc taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc atttcttgca
     4681 ttactaaaaa cggcgatgac aaattttttt tattttcgat tatctttcct ttggtagggc
     4741 gaataatctt catgagtatt cttagtaagg ttgttttccc cacaccgttt ttccctacca
     4801 aaccaataac atctccaact ttgacatcaa aatttatgtc ttttagaata ttttcataag
     4861 taatttcttc aacttttaat acatctgcac ttaccttttc gatataggga acttcaatcg
     4921 aactattaaa aatgtcaaat ttccttaatg cgttattgtt ttcagttttt aacaactgaa
     4981 tagttgtagt ttttacagtt ctatttttaa caaatagaac ttgatcaatt aattctttca
     5041 aatagtaaaa acgatgttca gctataacaa tagtaaatcc tttatttttt aaatcacaaa
     5101 gtaagtcagc aagtttctta gtcgctttga agtccaaatt tga
//
LOCUS       DAZUJO010000139         4946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000139 DAZUJO010000000
VERSION     DAZUJO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.146"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..828)
                     /locus_tag="ACG8OL_002819"
     CDS             complement(55..828)
                     /locus_tag="ACG8OL_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS4818720.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            972..1322
                     /locus_tag="ACG8OL_002820"
     CDS             972..1322
                     /locus_tag="ACG8OL_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4818721.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1303..2019
                     /locus_tag="ACG8OL_002821"
     CDS             1303..2019
                     /locus_tag="ACG8OL_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS4818722.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2019..3467
                     /locus_tag="ACG8OL_002822"
     CDS             2019..3467
                     /locus_tag="ACG8OL_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4818723.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3537..4046)
                     /locus_tag="ACG8OL_002823"
     CDS             complement(3537..4046)
                     /locus_tag="ACG8OL_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818724.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4036..4761)
                     /locus_tag="ACG8OL_002824"
     CDS             complement(4036..4761)
                     /locus_tag="ACG8OL_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818725.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1699 a          842 c          998 g         1407 t
ORIGIN      
        1 ttaagaaaaa ataagcgatt ttcttaagaa aagaggtagc ttcaaaaaaa gatcttaaat
       61 cagatcaaaa tacttcaatg cgtggacaat tccgccttct gtatttttct ttgtgataaa
      121 ggtagaaagt tcttttaatc cgtcataggc gttgtccata gcgattttgt tgtcgcaagc
      181 ttccaacaag gcaaaatcat tcggaccatc gccaaatcca aaagtaggaa ctttttccag
      241 ctgaagttct ttttgcagac gtttgacacc tgcccctttt gagattcctt tcttcactgt
      301 atcgatcgag taaggaccat tccgataaaa agttagctca ggaaaatgtt cgtgatagta
      361 ttcgtcacct gattgagata agatcagcaa catatttact gtgttttctt taaagcctaa
      421 aggatcaatt tccggaacat ctgaatgaat gaagtcgtaa gcattgatca ttgtttggct
      481 atgaccggta caccagattt tctgatcgtt atagtaagaa agttcatgac cgttcaactg
      541 tacatgatca tacatttttt gacattcttc tgaagaaaat gtttgagaat agatttcttc
      601 gccgtctact cgaatgaaag aaccattcat ggtgaccgca gaagtaatgc ccgcatcttc
      661 catgatagag tggatttctg cttctgtacg tcctgtagcg atgacgggga gaacattgtt
      721 ttcttttaat gcgttcatag cttttgccac ttcgggtgtg atttttgatt tcgcatctag
      781 taatgttcca tcaagatcaa agaaagtgat tgcacgataa tttgacacaa ataaaactcc
      841 ttttttattg gaataagttt atcttaacaa agaatgaaca aaagacaaac ctttcgtgaa
      901 gaattcctga acatattggt tcagtcaaca atttagagta tactataaga agagaattta
      961 ggagggaaag aatgattttt ttcagtattg gtatcttatt cttaataatt ggcagtcttt
     1021 ttcggattct gccctcaaaa ggaaatctgc ctttttatgg gtatcattcg cctcttgccg
     1081 caaaaacaga cgctcattgg cggctggctc aaaagacaag cggaaactgg ttttttctta
     1141 tgggcttact gatggcattg atcggctatt atttaaagac atctgggcat acgaattatt
     1201 ttctgattga aatgcttctg ctagtatttc caattatgcc tatttttatt atgacagaaa
     1261 agaaattaca gaaatatgac ctggaaactg gagggaacga taatgaatat tttaatgatt
     1321 gaagataatc aatctgtttc tgaaatgatg cagatgtttt tcttaaatga aggttgggaa
     1381 gcaactttca agtatgatgg aaaagaaggg ctcgatgcct ttttagaaaa cccacaacat
     1441 tgggatatga ttacactaga cctgaatttg cccacactgg acggtatgct agtaggaaga
     1501 gagatccgca aagtttcaag tactgtgccg attatcatgc tgacagctag agattctgaa
     1561 agcgatcagg tgatcggctt agagatgggg gcagatgatt atgtcacaaa acctttcagt
     1621 cctctgacgc ttattgctcg aatgaaagct ttgcatcgcc gtagtgagtt ggcagaagca
     1681 agagaagaag cagcagtttc agatgatcat tttgatatcg agactagtca tttcaagatg
     1741 aatacgaaaa cacgagaagc gtatctggac aaccggccaa ttgaaggact gacgcctaaa
     1801 gagttcgatc tattgtttac tttagcaaaa aaaccacgtc aagtattctc tcgggagcag
     1861 ttgcttgaat tagtctggga ttatcagtac tttggtgatg aacggacagt ggacgcccat
     1921 atcaagaaat tacgacaaaa aatcgaaaag gtcggaccac aagtcatcca aactgtttgg
     1981 ggagtcggct acaaattcga tgattctggt gttgcctaat gcgttatttg tatcagcaac
     2041 tcttagcttt ttgcggaatg gtcgcgttga tcttattgat cgttggagtt tcttttacac
     2101 aactgacaaa acaaacgatt gaagaaaaca attaccagca actgtttgga tatgctgaat
     2161 cagttgaaaa gacaacgcaa acctatgccg atacgctccc gcagtttagt caagatcagg
     2221 cgtttcaaaa tgcattgctg ctgacagagc aagtactgac agaacaaaat gtgaacttta
     2281 tatttatcga taaaaacgaa cgtgtcattt atccgactaa tacagcttta ctgaatttta
     2341 ccttaacagc tgatcaatgg aaaaatctga ggaatggacg tcaagaaaaa ttcacatcga
     2401 ataaaaatat tttaggacaa gcacaggcga cctcttacgc acttgtgcca tttaatttga
     2461 atcatgaatt ctatggagca cttgtagtta gtcaacctgc gcgaaatatc gataacagcg
     2521 ttcgtccaat tacactgaac ctgttcaaag gatttatttt ttccagtatc gttgcagtta
     2581 ttgccagcta tgcttttgca gcattccaag ttaagcgtat caatcggtta aggaatgcga
     2641 cgaaagaagt gacgaatggt aattttgatg tacagctccc cgtacatgac aaagatgaat
     2701 ttgatgagtt agcagatgat ttcaataaaa tgacgaattc attgaaagaa tctcaagcag
     2761 aaattgaaga acaagaaaat cgtcgaagac aatttatggc agatgcttct cacgagatga
     2821 gaacacctct tacgacaatc aacggacttc tggaagggtt agaatacaat gcaatccctg
     2881 aaaatcagcg agaaaacgcg attaaattga tgaaaaatga aacagagcgt ctgatccgtt
     2941 tggtgaatga aaacttggat tatgaaaaaa tccgtacgaa tcaaatcagt atggtcatta
     3001 aaaaattcaa tggaacagaa acattgaaaa atattgttgc acagttagaa gctaaagcag
     3061 aagcagcagg agatacattg acattgaaag ccgttgatga tattgatgtt tacgctgatt
     3121 atgaccgatt tgtccaaatc atggtcaata ttattcaaaa cgcgatacag tttactgaaa
     3181 acggccaaat cacgattact ttagaaaaag gctatcttga aacgatcatc acgattgaag
     3241 atacgggaat tggaatgtct gaacaacaaa tgaaaagtat ctgggatcgt tattataaag
     3301 tagatccttc aagaaaaaat acaaaatatg gtgaatcagg attaggttta ccaatcgtgc
     3361 agcaacttgt acgcttgcat aaaggaaaat tagaagtaga aagcgaacta ggaaaaggaa
     3421 caaaattcgc tgttcgtttg cctgacgtag aaatcaaaaa agagtaaaaa acaagcggta
     3481 taaaagatca tgaatcgatc ttctataccg cttgttttta ctataaaatg attgatttag
     3541 cccgcgggct ctaaacgatc aatataagga ctttgagcaa tagtagcatc atgagtaaag
     3601 agtaagatac cttgattttc ttcatgagct aaataagaaa gcaagtggag tagctcttgg
     3661 atctccaata cactaagttc atcaaatgta tcttcaataa caatattgtt tcgcttggaa
     3721 agcaaggcat gaagtaattg cagctttact ttttcaaaag acgtcaactc agctgccgca
     3781 caattaagca aggaaggaga gatatcaagt ttttcttgat aggccaaacg atcttgtttg
     3841 atttcttttt ccggtacatc caacaggaca tggtctttaa gattcagata aggaaacatc
     3901 ggccaatttt tttggatgat cgcaaaatta tccagttttg caacaagaac ctcttttttc
     3961 aagtgctcca atattttatg ctgtttttcg agaggacaca aaataacggt caagtgttca
     4021 gcatgaatca aatattcagg tttcattacg tccaggcctc ctactttgca aatgatggat
     4081 aaaaaaagtg aaagggaata gactaagaat gagacagcat ccactgagcc caataagagt
     4141 ttgggcaaat cccttaagta tatctgtgaa aaaaagatca ttttggaaat cgatggtaaa
     4201 aaaagtctgt tgatccatcg gaattgtaag tccgcttacc gaagtcaaga tatcacggat
     4261 atctggcagt tcagtagtct ttaagacaag aaaattgatc ctttgaagaa gcgcttcata
     4321 aactgactgg aagctgacga tccacaataa aaccaagata caacaaagaa agagaggaaa
     4381 gaacagctgc cagaaaataa aaaggaataa tttggtttta gaaagaccag tcaggcgcca
     4441 attgatcaat tcctgcttgt tttgcctgac tgaatagtaa aagaaaaaga taaaaaggaa
     4501 ggcaaataga aaaaataaac tgaatatgcc ttttatataa aatcggctag ctaattctaa
     4561 agccggaaga agctctgtag atgcagaagc gccaataaat gaaagccgat cttggacttg
     4621 attggctaaa gccgattgcg aatggtaaag agtcaggcat gctatcaata aaaaagcaga
     4681 aaagcttgaa aataaacaat agagtattgt ctgctttttg taataaatta atcgagcagt
     4741 agcatagcgg attattttca tacttttcac ctctataaaa agtatagaaa atgaatatga
     4801 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
     4861 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
     4921 tgtttattcg aaaagaggag cagcga
//
LOCUS       DAZUJO010000140         4931 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000140 DAZUJO010000000
VERSION     DAZUJO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.147"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8OL_002825"
     CDS             85..972
                     /locus_tag="ACG8OL_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS4818726.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8OL_002826"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8OL_002826"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS4818727.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8OL_002827"
     CDS             2274..2720
                     /locus_tag="ACG8OL_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS4818728.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG8OL_002828"
     CDS             2993..4345
                     /locus_tag="ACG8OL_002828"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS4818729.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG8OL_002829"
     CDS             4516..4803
                     /locus_tag="ACG8OL_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4818730.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DAZUJO010000141         4896 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000141 DAZUJO010000000
VERSION     DAZUJO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.148"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..457)
                     /locus_tag="ACG8OL_002830"
     CDS             complement(143..457)
                     /locus_tag="ACG8OL_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818731.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            743..982
                     /locus_tag="ACG8OL_002831"
     CDS             743..982
                     /locus_tag="ACG8OL_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818732.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1344..1604)
                     /locus_tag="ACG8OL_002832"
     CDS             complement(1344..1604)
                     /locus_tag="ACG8OL_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818733.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1789..2286)
                     /locus_tag="ACG8OL_002833"
     CDS             complement(1789..2286)
                     /locus_tag="ACG8OL_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS4818734.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2416..3126)
                     /gene="budA"
                     /locus_tag="ACG8OL_002834"
     CDS             complement(2416..3126)
                     /gene="budA"
                     /locus_tag="ACG8OL_002834"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS4818735.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3139..4803)
                     /gene="alsS"
                     /locus_tag="ACG8OL_002835"
     CDS             complement(3139..4803)
                     /gene="alsS"
                     /locus_tag="ACG8OL_002835"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS4818736.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT         1479 a         1005 c          748 g         1664 t
ORIGIN      
        1 taaactctag agaaattaat tcatcctaac ataaaataat tgttcactca aaagtttttc
       61 tagaacaatt aaaacagcag gcttcacatt cgctatacat ttgtaagaag taataattcc
      121 tgtactaaaa ggcataattg ctttaattat tttttgactt ctcttcaaac acaagaagta
      181 gttgtgtaat aattgcagaa ataaggattc caataataga tataaaattt gacatggaaa
      241 cgtctataac attaaaagaa taaagtgttg aagaaagtaa actgattgaa ataacccctc
      301 cgtaaaaaat agtctgtgaa tatacccact ttttttcaga agacgtactt cgtttggttc
      361 taaaacctat ccctgcattt ctactcttat caaaaaacca aatatttaga agtaataata
      421 ttgctattat taagataaaa ttactgtaaa aatccaaaag ctatcctcct agcaactatt
      481 taaataccca aaaaaattac ctctagataa tatcatacct acgttttcac ttataaattt
      541 tttcataaaa aaagcgttta gaggcataat cgttaataca gatggtaacc aatgtatctg
      601 gtttgaactg ggatatcacc ctctccatat atgtattact aagactaata gatactataa
      661 aagacttgat agttttgcat tgattttgcc taatactgag cataggtgta tgctaaacga
      721 tactataagg agtgtgtaca taatgaaaag aattctgttt atcagtttat caaatttatt
      781 gttacttact gcttgtggga cttctgcatc aaagaacata ccttcagata cgcaaatttc
      841 gaatagtatc gattcaacaa tttctacaaa tgagaaaaat tctccggtag aaacttccga
      901 tataaaagtt tctacctctg caaaaaacag ggtaaagaaa ctgtcagcac gtctccttta
      961 tttcaaacaa actctgaatt agcttcttca tcttcttcag caaataatga tgatagtttc
     1021 gcgtttactc ctaactaaat gcaaccttta caaatgcttt acgataacaa gcctgagcta
     1081 aatgatcctg atatcagttt tgcatttttt gaaatgattg gatcggatga tctttttaaa
     1141 actactagtt tgtctatccg aaaataggac agttctggca gtataggttt ttatcgagtt
     1201 tctccaccag gcctcgttac agagacagat tctctaggcg aaccttttga ataaatcaaa
     1261 aatcatgaac aatcactgcc aactaaataa agtgccttca aaagcttggt cttctcgctt
     1321 ttgaaggcac tttatttaaa tctttatgaa actaaagtat gttcatattt gttgctatag
     1381 ttatatagat attctgttgt ttccaactca taaacgtgag tatattttgc cactactttt
     1441 ccgcattttt ctaatatttc attgcgatcg acgtcatgat attcgtctac gcagatcaca
     1501 acacaatttt ctaatttact gattacttct ccaatcactt tttgtttcaa gccaataggt
     1561 tggcaaatat atttttttcc taaaatgata gtttcgttat tcatatttca tccatcctta
     1621 atgttttttc attatttaaa atgataataa cattaaaata tgattatgaa aatcaataac
     1681 acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag aaatgcccac tgtctttccg
     1741 gctcttttta ttcttgattt ctcttctttt ttgtttcaat agacgatttt agttattaat
     1801 aaatgaaaaa gcatttatat taaaatttgc taataaaaga tctacatatt tttctagaaa
     1861 tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta aaacaccgat
     1921 taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg aatcgcagga
     1981 aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt tttcagctga
     2041 ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg
     2101 tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag tctcaggcaa
     2161 agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat cttctgcttc
     2221 taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct gtttttctgt
     2281 ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc agtttttttg
     2341 taaacgctag gttttctcct caaaaaaacg agatacgtca ggttaatcac cttgaacgca
     2401 tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag cataatcaat
     2461 cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg
     2521 gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc caaatgtatg
     2581 tgtatcatcg ataaaatgca ggtgaaatcc cgctgcagca actccttgga ataattcagg
     2641 cgtaaagaac ccgactaatg ttccttggac attgctttgt tggaattctg gttgagactc
     2701 tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat
     2761 atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg cttctcttac
     2821 taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa aactagtatc
     2881 tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta attgggcaac
     2941 tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt caccatcaag
     3001 cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa tcgtttctgt
     3061 tccatccatc aagccagcca tcaaagctcc taatgtcccg tgctggtata agattttttc
     3121 agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc ccaatttctc
     3181 attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg caaatccttc
     3241 ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag cgccaaaagc
     3301 ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc gctgatattt
     3361 cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca aatgaacgat
     3421 attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa atcccccatc
     3481 gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg
     3541 caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag gttcataact
     3601 tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa cagtcatatc
     3661 atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc
     3721 tccttttgat tgaacaatgt cccgctccgt taattctgct tgaagagatg ctaaatattc
     3781 ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat caagagtggc
     3841 tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt cagctggcgc
     3901 ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg cttcatattc
     3961 gattggatca tagccaatag caataactag atcacttcgt ttcaaaagca tatctcctgg
     4021 ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac gtgagatcac
     4081 tccagctgct tggaatgtct ctacaacagg caatgctgtc tttccaacta attgcctgat
     4141 ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg gtagttttgc
     4201 ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac ctaatttagg
     4261 atcgctcaat ggcttgatga cattcccttg gacaggtgca tccactacat cttgcgggat
     4321 actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat tggcaatcac
     4381 ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg gctggaacag
     4441 tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc
     4501 ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg tagctaagtt
     4561 actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa tacgtccgat
     4621 tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt caggaccttg
     4681 atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga atacataatc
     4741 aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca ttttttcgtc
     4801 cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat aatcacaaat
     4861 aattgataca gtttctaaac ctgtttcatt caaaag
//
LOCUS       DAZUJO010000142         4806 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000142 DAZUJO010000000
VERSION     DAZUJO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.149"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..591
                     /locus_tag="ACG8OL_002836"
     CDS             <1..591
                     /locus_tag="ACG8OL_002836"
                     /inference="COORDINATES: protein motif:HMM:NF015839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HFS4818737.1"
                     /translation="KSEQDRNERTNILMKRLEASTSNFNNKLDVFEEKTKHARLNFET
                     TAKHYIKRLDEDNLKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDH
                     NKVVDKSNTALKVMTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDH
                     ESAWRYLMLILYAVPYVFFAIILWLIALFFRVFNDL"
     gene            1267..1521
                     /locus_tag="ACG8OL_002837"
                     /pseudo
     CDS             1267..1521
                     /locus_tag="ACG8OL_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1591..2103
                     /locus_tag="ACG8OL_002838"
     CDS             1591..2103
                     /locus_tag="ACG8OL_002838"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818738.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2100..3137
                     /locus_tag="ACG8OL_002839"
     CDS             2100..3137
                     /locus_tag="ACG8OL_002839"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4818739.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3297..3473
                     /locus_tag="ACG8OL_002840"
                     /pseudo
     CDS             3297..3473
                     /locus_tag="ACG8OL_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            3528..4806
                     /locus_tag="ACG8OL_002841"
                     /note="aph(2'')-Ia"
                     /pseudo
     CDS             3528..4806
                     /locus_tag="ACG8OL_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
BASE COUNT         1830 a          703 c          918 g         1355 t
ORIGIN      
        1 aagagcgagc aagacaggaa cgagagaaca aacatactca tgaaaagact agaggcttcg
       61 acctcgaact ttaataataa gttagatgtt tttgaagaaa agactaagca tgcaagatta
      121 aactttgaaa caactgcaaa gcactatatt aaacgacttg atgaagataa tttgaaatta
      181 gattttcaac aagctattca agatgaatta agcgatacta aagacgaaat acgtgaagtg
      241 acgaaacaag cgagagaaga aacaaaagag tataaagaaa tcttagaaag taaaataaaa
      301 gaccataaca aagtggttga taaatcgaat acagcgctaa aagtgatgac aaaaggcgtt
      361 acaaatatat tttttgtgct aattattttt gtacttgtaa tgcttgtgac aggtccgata
      421 ggtcatttct ttggtattga acatttatat agctttatta atggttttat tgatgaccac
      481 gaaagtgcat ggcgatattt gatgttgata ctttatgcag taccctatgt attcttcgct
      541 atcattctgt ggctcatagc attgtttttt agagtattta atgaccttta ggtagattta
      601 tatataaaaa gggctaaata cgcaaaatac ccccttaaaa tgcaaaataa atgcaaataa
      661 gggggtaact ccttatgaaa aaacaactaa tagtattata acacgttttt atgccgagaa
      721 aacttattgg ttggaatggg ctatgtgtta gctaacttgt tagcgagttg gttggacttg
      781 aattgggatt aatcccaaga aagtaccaac tcaacaacac ataaagccct gtaggttccg
      841 accaataagg aaattggaat aaagcaacga aaggagttga gtaaaatgag tgagaaaacg
      901 aagaaaatca ttcgagtatc attagctttt tcacaagtag tagtgcaatc attgatagtt
      961 tattatactt accagcaata taaattatct aaagagaatt aagttttgat tatgtaataa
     1021 gttttggtac taaaaaagac ttgatctaat tagaccaagt cttttgttag tgttatatta
     1081 ataacaaaat aaaaataaga agtcgctcac tccccgacca aagtttgtga gcaaaataaa
     1141 cagtcgatgt ttgtttctta attgtatatc ttgatattaa acgatttaag attattcttc
     1201 aagatatata ttcgcaaatg taattggaaa agcgacttct taaacaaaat taaggagtcg
     1261 ctttttatgg caaaaataac gaaaaatgag caagaaaatt tggtggaaaa ttacacaaaa
     1321 aaaccggcgt actctaatag ccggttagac gcacatttcg tgtgcacttc taatccgaaa
     1381 ttgagctttg acgctatgac gatagtcgga aaccttaata aaaacagcgc taaaaagcta
     1441 tccgatttta tgagtttaga tccacaaatt agactttggg atatacttca aacaaaattt
     1501 aaagctaaag cactacaaga ccccctatgt taggctagtt gtcgtatagg ataataagtt
     1561 ataataaaga ctatacggag gatgattacc atgacaaata aaaacaatga acgttactct
     1621 accgaactga aagcgacagc catcaagaaa atgatgccac ccgaaaatag ggggattcgc
     1681 caattaagtc aaactttggg cattccagaa gcgactcttt gttcttggcg taaaaaagct
     1741 agaattcaag ggaatactac acccggaacg aaaaaaaatg tcgaaaaatg gtcaagtgaa
     1801 gataaatttt taattgtgat ggaatcattc tcaatgaacc aagtagaact tgcggaatat
     1861 tgccgaaaga agggcttatt taaagaacaa attgaggctt ggaagaaaac gtgcttatct
     1921 gcgaatgaac aggaagaaaa tcgaacgaga gaacttgcaa ctgaactaaa agaagagaaa
     1981 aaacaagcgc gtcaattaga gaaagaatta cgtcataagg aaaaagcatt agcggaagcc
     2041 gctgctctct tattactaag aaaaaaagca cacgcgattt ggggggacca agaggaagaa
     2101 tgatttcatc ttcagatcgc aaacaagctg ttgaacttat tcaagaagcc aatcgcaatg
     2161 gggcccgtct cacctatgcc tgtaatgagc tgaatattag cgttcgaacc tacgaacgat
     2221 ggacgcgtga aggtacgata gcgcatgatc aacgcccttt ggcgaaacgt cctgtaccca
     2281 aaaataaact gacggatcaa gaacgcgaaa aaataataga aaccgtttca aaaaaagaat
     2341 ttatgaattt gccacctagc caaattgtac caaaacttgc ggattgctcg atttatattg
     2401 catcagagtc tagcttctat cgtgtattgc gagaaaaaaa tatgcaacat caccgtggcc
     2461 gaagtcaagt ttcacaaaaa cgcataccgc caagtcacct tgcgacgaag ccaaatgaag
     2521 tttggacttg ggatattact tggctaaaag gtccaataaa aggactcttt taccggttat
     2581 atctcatcat tgatttattt agccgtaaaa tggtaggctg ggagatttgg gaatcagaag
     2641 aagctcgtca tgccgaaaaa cttattaaac agacggtgct taaggaacgt atccaaggac
     2701 agccgttggt tttacactcc gataatggca gccctatgaa agccgcgact tttcaagtct
     2761 tactcgaaaa attaggaatt caaagttctt attcaagacc acgagtgagt aatgacaatc
     2821 cttattcgga agcggctttc cgaacgttaa aatatcgacc agaatatccc aataacggct
     2881 ttaaaacaat agatgatgct cgaaaatgga cgaaagagtt tgtcgattgg tataacgatt
     2941 ctcatttgca tagtgggatt caatttgtgt ctcctgcagc gtgtcatgaa ggacgacatc
     3001 tcgctatttt agagaaacga aagcaggttt acgaggacgc taaaaagcgc catcctgaac
     3061 gctggtcaaa aggtgttcga aattggactc cacatcaatc ggtggcgttg aatcctttaa
     3121 aagaaacaga aagttaacag gagcataatc tttcaagatt atcaaataac cctcgtcacc
     3181 caatagggga aaaagttatt cttcccctat tggataacga ggccaaccga ccatagggag
     3241 gttctgcttt ttgataggtg gaaaataaaa atacgacaac tatattgaca aacaccgacc
     3301 atcgtccagt aaagagacgc aataaattct atcgaagttt acgcactgcc tctacaacga
     3361 ttaaaggcat ggaagccatc cgaggattat ataagaaaac ccgaaaagaa ggcactctct
     3421 tcgggttttc ggtctgtact gaaatcaagg tattattggg aatcccagct taaatcatag
     3481 ataccgtaag ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa
     3541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
     3601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
     3661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
     3721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
     3781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
     3841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
     3901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
     3961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     4021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     4081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     4141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     4201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     4261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     4321 aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg
     4381 tattagaaga gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa
     4441 aagattatat agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa
     4501 agtgtttatg ccataatgat tttagttgta atcatctatt gttagatggc aataatagat
     4561 taactggaat aattgatttt ggagattctg gaattataga tgaatattgt gattttatat
     4621 acttacttga agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa
     4681 tgtatggaaa tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt
     4741 atcctattga aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg
     4801 gtagaa
//
LOCUS       DAZUJO010000143         4744 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000143 DAZUJO010000000
VERSION     DAZUJO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.150"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1026)
                     /locus_tag="ACG8OL_002842"
     CDS             complement(28..1026)
                     /locus_tag="ACG8OL_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4818740.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1101..1397)
                     /locus_tag="ACG8OL_002843"
     CDS             complement(1101..1397)
                     /locus_tag="ACG8OL_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818741.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1390..1683)
                     /locus_tag="ACG8OL_002844"
     CDS             complement(1390..1683)
                     /locus_tag="ACG8OL_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFS4818742.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1829..2380)
                     /locus_tag="ACG8OL_002845"
     CDS             complement(1829..2380)
                     /locus_tag="ACG8OL_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFS4818743.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2554..2853)
                     /locus_tag="ACG8OL_002846"
     CDS             complement(2554..2853)
                     /locus_tag="ACG8OL_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFS4818744.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2880..3155)
                     /locus_tag="ACG8OL_002847"
                     /pseudo
     CDS             complement(2880..3155)
                     /locus_tag="ACG8OL_002847"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            3493..4176
                     /locus_tag="ACG8OL_002848"
     CDS             3493..4176
                     /locus_tag="ACG8OL_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS4818745.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4213..4710)
                     /locus_tag="ACG8OL_002849"
     CDS             complement(4213..4710)
                     /locus_tag="ACG8OL_002849"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS4818746.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1404 a          907 c          694 g         1739 t
ORIGIN      
        1 tttttctttg ataaataata ttttcaatta tttaatcatt tgaaaattgt tagtcattcc
       61 aatacgttga gaaattcttc catctgtata aacacgaaat tgttttccac cagccatggt
      121 cgtgtttcca tcatcatccg ttacaatatt aattgaatag tagcttccat tttcatcttg
      181 cattagatct ttcatatatt catttgtgct aattaaatta gtatttgata catttttatc
      241 aggagaatca tacaaccata aagctttatt atttagcaca gattctttag cagcttccca
      301 acttgaaatt cctgaactac ttgtagattg tgtcgttgta gttgttgctt gttgagtaga
      361 ttcttctact actggtgttt gaggagtttg tccattcaca atatcactga aaccttgttt
      421 taactgttga ttttgagttt cgttaaagta cagataatta ttttcattac cttgattttg
      481 ttgcgttaaa taaactttag gttgtccatt ttgaaaaccg aaaaagtaaa catgctgccc
      541 taaatattct ccagtttcta catcagaata aactgctact aaattgaaat ctgcatgacc
      601 agttccatct tctgaccatt gtacggtaac tggtgcttca ttgattgcca ttttccaatc
      661 gccattaatc acggcttgtg gtaaaggagt gccatacaga cttacattat tctgaagatt
      721 gtagctttta tattcttgac ctaatgtctt tccccattgt gtgacaaaag tttctaattt
      781 ggaagcttta ttcgtatccc acaaagtatt cttaacctct tcagaagaag attcagtgat
      841 tgaacttgca cttgtttcca cagattgctt catttctgtt gtagtttgtt cagttgattg
      901 cgttttactt tttttagtct caattgttgt atcagtttct atagtcgctt tagtcctcgt
      961 attctcagat gtacaaccag tgaataaaat tcccattcct attagtaata acagccattt
     1021 tttcattata tttccccctt tcaatttctc cctcacgtac ttattctatc agacaattaa
     1081 ttgatctaaa tggtaatctt ttaatcgatt ctttcccaat aatgtcgata gcccgtatgg
     1141 cagcggtctc ttccttgagg actctcagat ggattaaaac tagtagaaat ttcccttcca
     1201 catcctctac agcgataagt tctcattatt tttgggctat tagtattatt tttcttagtt
     1261 agagctatga gtccaactat gattacccca actgtcttaa ttacttttcc tttatctata
     1321 ttcggatgcg caagcatata ttgatataac caatatccac cgtaaattaa accacctaat
     1381 acaatcagct tagccatgta ctcgtctcct ttctttttgt ttaattactt tttgaatcca
     1441 tcttgcagag aaaccataaa cttctgtcaa ttgacggata tctacagttt tttcctgttc
     1501 tatcatgttt tcgattcttt ttctaactaa ctcacgatcg tacattttca ttggaaaaga
     1561 aatctgttgc cctcgaaaag caagataaaa ttgatagagc aattctgaat ccagttccat
     1621 ttcctcaaaa aagtcattca tacttttata agagttactc aaacaagttt catcaatctc
     1681 catctacaca ccccctgtta ttttttacat aatgaaaata gcatattact gggtacattg
     1741 ataattcata taacatgtat catcacatgt atttttaaag tcaaaaagca tgtaaaacta
     1801 cttataagta acatgagaaa ataaaatgtt atttttcttg aaataataat tgactatctg
     1861 ccaattgcca ttcattacga acatgaatac ttagtgggtg ggataactta ccttctgcat
     1921 ttatcaatac tttggtcatc ttcttttgtt tcgcactcaa ttgatcgtaa atagtctttg
     1981 cttgttcata agccacttga ctcgtcgctg taatcgaacc acaagcaaaa ataatggtat
     2041 tgattttctt ttctgtaaaa acagtcgtta atatttccaa agattgttca tcatttcctc
     2101 gaagaaatac ccgaggactt ttcgcttttg tgtacgaaaa aagattcaca gcaataaaac
     2161 cagtaaatcc taattgtcgg atattctttt cgattaataa catactcaaa tcattcgtaa
     2221 atggttctgt atctgttgga cgaatagtaa tgactactgc taatttatct tccgcttctt
     2281 ttcctttgat cgcccattgt cgtttgaaaa catagcgatg ggtatgatga ggatctcgcc
     2341 aaacttctgt ttgtgtggtc actaattctt tttcaatcat ctctttttct cctatgctat
     2401 tttttgttca agcacttcaa ttaccattgc cgttataagt gtcaatacta gttgaaaata
     2461 tttcatattc tttctccttt tttcttcaga atttgatgaa tcgcggtaag gatatcttct
     2521 aaacatttcg caatcattct caaataatcg atcttattca tccgttcctc caatctgact
     2581 ccaaaccaat cgtccttcta ccagtaaccc acgaacaaat tctcgctggc tggtaatatc
     2641 tgtaatgaaa taaactacat agtcttttcc atcttctggt gcgcaataaa tataaagatg
     2701 tgtcatcact aaacgtggta gatgaacatc ttcaggaagc atacccattt caatttcctc
     2761 tttttccatc gtaacggatt catagcccca ctcttcagaa gcagtgacca gctgtgcaca
     2821 cttttccagt acttcgatag ccgtttctaa caagtccttt cgttcctttc ttttagatac
     2881 tagtattctt ctggtaataa catcgtttga tactcacccg aatcaatggc ccatattttt
     2941 tgagacactg atttcaaacg aagcttaggt gaaatcagat actcttttct ttcttccggt
     3001 tcttcttgac gatgaaccaa cttttgattt cctttttccg tagaaaattc aaaaatttga
     3061 agatagtcta aaggaaattg cttttgaacg ttttgatcga ttagattcca acataaagct
     3121 tgtaattcca ccggcaaact gtccattact ccctttgtga cataacgtta tcctttattt
     3181 tgaaacattt cttttccctc ctttgccata aaaatagccc aatcttctat taaagaagat
     3241 tgggcttcat ggatataata tatatttgta aataaagtta attcagttat aattatcaaa
     3301 actacacaat tattaaagtt tttcatatct ctttgtttag taagtttttc taaatcttaa
     3361 cccgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg gtaaatcaac
     3421 aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg aggactattt aacaaactac
     3481 gatggttacg tcgtgacaac tgaacatttt gacttagcca atcctgttac taaagtcgat
     3541 gatatccccg attatgaaat gtacagtcaa acaatcgata gcttaaataa gcgttttggc
     3601 aatcgtgttt taaaaggcat caaaatcggt tacatcgctt ctgaaaagga tcggatcatc
     3661 gactatttag ccgataaaga ttacgattta aaattactca gtgtgcatca taatgggcaa
     3721 tttgattatt tagatgacga agtcaaagat atggatcctg ctattgtgat tcctcagtat
     3781 tttgcacaac tttcagaagc actagttgtt atagaagcag atgtctttgc tcattttgac
     3841 tatggttttc gggtatttgg tctatccgtt gctgaattta aacaatatga agcgcagttt
     3901 ttaccgattc tagatcaagt aatcaaaaat aaattggcat tcgaattaaa tgctaagagc
     3961 gcttacttgt atgacaattt agcgttatat gaatatgtca tcgatttata tctttcacgt
     4021 ggtggtacct tgtttagtgt tggatcagat gggcattacc ttgaacattt ccgtttccat
     4081 ttcgatgatc tttttgctct gttaaaagca aatggtgtga cggaattagc gatttatcaa
     4141 aaaggaaagc gaattatggt tccattacca gtctaaaaaa acgtcctcag catcaaaagt
     4201 gaggacgttt ttttagattt ctttttctaa aatcggacaa atacgatctt gttttcctaa
     4261 aggaaaatgt ttgatcccga tttttttaaa tccatgacgt tcataaaaac gaatacctgc
     4321 atgatttttt tcataaacag tcaaacgtaa ataacgtttt tgtttcgttt tagcataacg
     4381 attcgcttca tcgagtaacc gttgccctaa ccctttgttt tgataatctt tgagcagata
     4441 gagccgttga atctctatag catctttttc atcattgact ttcatataac caatgagtat
     4501 ttgatgatcc ttaataccaa taaatttact ggttgactgt gccaattcta ctgccaatat
     4561 ttcagttgtc aattgtgtct gtaaatatcc ttgcatatca agtggatctg cgccatcgat
     4621 aaacgattca taaaaactga caacacttac aacttgtaaa gcatctagat catcataatc
     4681 taaatacgct agtgtgattt gattgttcat ctaactcctc cttttgacat agtatactat
     4741 aaat
//
LOCUS       DAZUJO010000144         4713 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000144 DAZUJO010000000
VERSION     DAZUJO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.152"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..1157
                     /locus_tag="ACG8OL_002850"
     CDS             84..1157
                     /locus_tag="ACG8OL_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HFS4818747.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIK
                     NFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVD
                     FGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGI
                     GKLRLDGSNIQQNKPYVLGKVVI"
     gene            1201..1470
                     /locus_tag="ACG8OL_002851"
     CDS             1201..1470
                     /locus_tag="ACG8OL_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818748.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1702..1998
                     /locus_tag="ACG8OL_002852"
     CDS             1702..1998
                     /locus_tag="ACG8OL_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818749.1"
                     /translation="MEVRIRNVDEKIVREIDRRCNELSNRTGNKWSRNDYLKLLIEND
                     FDRPLMEYKKEKFDQLLEKFSEIQSYNTKILEEYVSQNNRILEILIEQNRGSEL"
     gene            2000..2422
                     /locus_tag="ACG8OL_002853"
     CDS             2000..2422
                     /locus_tag="ACG8OL_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818750.1"
                     /translation="MTKVIDKHIYFYQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            2441..4033
                     /gene="mobP2"
                     /locus_tag="ACG8OL_002854"
     CDS             2441..4033
                     /gene="mobP2"
                     /locus_tag="ACG8OL_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS4818751.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSKYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDSKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGIEKRNLLTRKSDLRNTLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTQYLLTKEPEYQEYIQ
                     ISKALREEANSLYGESKRKSKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            4045..4380
                     /locus_tag="ACG8OL_002855"
     CDS             4045..4380
                     /locus_tag="ACG8OL_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818752.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPYCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFTCKSI
                     EESISLTEC"
     gene            4526..>4713
                     /locus_tag="ACG8OL_002856"
     CDS             4526..>4713
                     /locus_tag="ACG8OL_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818753.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1806 a          675 c          944 g         1288 t
ORIGIN      
        1 ttcagatcat ttgaagctct atatatagct gctaaaaaat attttacagc tgataataaa
       61 taaaaaatta aggtgggttc tatgtgaaaa aaacgtatca aaagttttta tggattagct
      121 atatcgtcct atttatcgtt gggtttctcg tcagtgtgtc cgtgatcgtc ggaacgcttt
      181 ttcttttgtt ctctgacgaa gatctggtac ttaaggggtt aatgagtacg ttatcgtgtg
      241 tcatgttact ttatttatgt atacagatca acgaaattat gccccctcat tggcaagaaa
      301 agaagaaaga atttaaggaa gaaatcgaaa taaaatccaa tgactggatt gaatacacat
      361 ggcttcctct gaaaagaaat agcaaacgaa aggaattttg ggcattgcaa ttatggccaa
      421 taggaaaact ttttatttat atggcttcac ttatggttac tttaatcggt tatttggtgg
      481 tggtctatgg ggtggcctat tacttgttaa gtgagccaaa acagatcgcg gcgtcaaata
      541 ttttcacggc agtaatgaca ttaatgtgtg tcatgtgctt ttctattttt agttacttac
      601 tcgtccaatt tatcaggcaa aatttaacaa tgagtaaact tttcaaaagt ttattggact
      661 tacttatctt aggcagtatg ttaacgactc taacagatcg gttcgaacaa tctgaaaaaa
      721 cggtagaggg gataaagaac ttcttaacga cgattgatca atttataaat ggatatatgt
      781 atttgtttga agtaggcttt attctcactt tgtttggttg tgtatttata aaactcatta
      841 aaagatcaat cgaaaaagaa atggttattg atctaattcc gacgctgagc ggctatgagt
      901 attacgttga ttttggaaag tatgaggagt tgatccatac ggatatcgtt cacacgagta
      961 tccatgaagt aaccgttaag ggcatcagat ctactttaaa agatggacag gagacgaaaa
     1021 caatcgaccg aagaaaagag gtctacaatt tttataaagt tgaagcaagt ctacgatatg
     1081 gcattgggaa attgaggtta gatgggagta atattcaaca aaacaaacca tatgttttag
     1141 gaaaagtggt gatttagaac ttcctatgaa agatgaataa ataaggagaa tcttttgaaa
     1201 atgattcttc tttacagaat tgtgcttata gttggcacgg agttaaaaat cttaaagaag
     1261 gagcagttat tcaaagtgaa agaacatcat aatcagttac tatgtattaa aacgggtacg
     1321 atgaacattc aataccgaac agaatattta gaattaacga atcaaactga ttttttgtcg
     1381 attcgagagc aacataagca agaaaaatta atggctactt atagccaatt gattgatgag
     1441 tgggaacaat tcaaggaatc taaaacataa aattccattt aaacacctta ttttttaaag
     1501 agcataagga aacgtgggaa cactttttaa aaaagtgttc caataggctg tgcctattac
     1561 gtttctcaaa aataattgtg ggaacaaaat atttaaaaat aggatagtga aaggatagcg
     1621 aaggatagta aaaggataga agtaggatag cgaaaaggat aggctccttt tcgctatcct
     1681 ttttaaattg gaggaattaa tatggaagta agaataagaa atgtagatga gaaaatcgta
     1741 agagaaattg atcgaagatg taacgaacta agtaaccgta caggaaataa atggagtcgt
     1801 aatgattatt tgaaattact aatagaaaat gattttgatc gtcctttgat ggaatataaa
     1861 aaagaaaagt ttgatcagtt attagaaaag ttttcagaaa ttcaaagcta taacacaaag
     1921 atattagaag aatatgttag ccaaaataat cgtattttag aaatcttgat cgagcaaaat
     1981 agaggaagtg aactttgata tgacaaaggt tatagacaaa cacatttact tttaccaaac
     2041 acatatcgat gtgttggatg aaattatttc tactcatgca gaggttaaaa actatagtga
     2101 agctgttcgc tttttatgta tgaactatga tccagaagtg gagaaaaaag taaattccaa
     2161 tcaagcaaag ttgaatggaa tgagtaaaga aatttctatt ttaaccgaaa tggtttcaga
     2221 gtttgcagat cttcatttga aagatacagg aatactaatt ggtacaaatt caacggttta
     2281 ccaagaagca aaagaacgtg tggaaggaaa aattaaatcc aatcaaacca aaaaatattc
     2341 ccctcagtta aagaaacctg tgaatgaatc tgttcatgcc agagtagatc catttgatcc
     2401 aacgaagatt ctaaaaagat aaaagtagaa ggggaacatg atgaataaat cgcctgcagt
     2461 tattctcact tgtcaattta ctttgcccaa taaaaaatcc ttttcaaaat acattgatta
     2521 tatgacaaga gaaaaagcgc tggaagaaaa ggaacgacgt acgcccgagg aagaacagga
     2581 actgcgtcta atcaagaatg ctttagagga tttttatatt cctgaaggag aaactttttc
     2641 ggagagtaag acggataaag aattcagtga aacgtcgaat gaagcgcaat cgttgatgga
     2701 acatggaatc aattttgaca agttagagga acaagatttt accaaatatc tttcttatat
     2761 gacccgtcac tatgcgttag aacaaaagaa agaactgacg caaagtgaac aaaaagaata
     2821 ccaacgcttg aaccaagcaa taaaaaaata tcaaacagag ccacacaaaa aagcttcagg
     2881 aaaccttcct ggagtttttt ctatggcttc agacgaagta aagggaagtg atctgaagga
     2941 gattaagcaa atcttccaaa gagggcaaca aaaaggctca atcatttatc aagatgttgt
     3001 ctctcacgac aattcctatc ttgaaaaaat aggattgtac gattccaaaa cagatacttt
     3061 agatgaagaa ggattaaaag cagctggaag aaaaatgatg gagacattat tcgagaaaga
     3121 gcaactgaat gaaacaggct actggatggc cacgattcac cgaaatacta aacatatcca
     3181 tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa ccatacactg aggttgaaaa
     3241 tggtgtgagt tatatcgtac cgaagggtaa gagaagacaa gcaacattag acgcaatgaa
     3301 aaccaattat gtgcatgaat tagaacgttt ttcttatgaa aaagaccgaa tgataggcat
     3361 agagaaacga aatttattaa cgcggaaaag tgatttgcgt aacactttaa caaaagtggt
     3421 aaaagaacca gtacgttatg atcgacacgc aatgaactta ttaaatgaag tatataaaac
     3481 cttacctgac aaacgttcgg aatggcatta tggggatgaa aaacgaacca aactttcacc
     3541 atacacaaga gagaaactcg atgagttaac ccaatatttg ttaacaaaag aacctgaata
     3601 ccaagaatat atacagattt caaaagcatt gagagaagaa gcaaacagtc tttatgggga
     3661 aagcaagaga aaatcaaagg atgcagaaaa aaatgctctc tttgatttga agaagcgcac
     3721 aggaaatgcc attctaacag aactgaagaa acaggatagt caaataaaac ctttaataga
     3781 aagtatggat cagatgcaat ctatttacaa tgaacgatcc ttccgaacac ttaaacagtt
     3841 gccaacaaat gaatacaagt atagttacga agaattcatg aagcgaagga aacaattcca
     3901 acaaagagtt gtcacgcaac ggagtttgcg tcaattaaat cgaacgatta atgcccatga
     3961 agaaaaatac cacgcaagta aagcatacga agagcaacaa cgaagaattc aaatggaaca
     4021 agaaagacag taggaggaat agcaatgaaa caatatttag atcaatggaa agttatagaa
     4081 ggatcattaa gagaagaacg aatcgagcaa cttccctatt gtttagaaaa agagcatttg
     4141 tttcagataa gagaaatgtt gagaaacgaa cagtttgatc caaatcaatt tttagtcgtt
     4201 gagtacccag ctactggtgt gtactgttgt aatcatgtga agggtgaaaa atattttatc
     4261 attcaagaat atgagggcaa gttagcgcct tattatacta cttgggaaat gaatgaagag
     4321 gggatcaata acttcacgtg taaaagcatt gaagagtcta tttcactcac agaatgttag
     4381 ttgttaagtg aataaaagaa gtaaaagagt cctgaacgta attgtccagg actctttttt
     4441 tcaatgaatt gttttccgcc aattcaatga gtggtgcgat ttccgactac attatatcaa
     4501 aagaaagaga agggaggaag aagaaatggc ttattcaact attggagttg ttgttgaaaa
     4561 aagcagagac aatctcgttt ttgtgacaga aattcaaacg ggcagagctt ttgtcgtcac
     4621 agacaaagcg gcaaaagctt atcaaagtgg cgatatttta acgttaaatc tgactacgaa
     4681 gacgttcgtc gatgcggcag aagattatcc att
//
LOCUS       DAZUJO010000145         4590 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000145 DAZUJO010000000
VERSION     DAZUJO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.153"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACG8OL_002857"
     CDS             186..2855
                     /locus_tag="ACG8OL_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS4818754.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACG8OL_002858"
     CDS             complement(2946..3560)
                     /locus_tag="ACG8OL_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4818755.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3679..4218
                     /locus_tag="ACG8OL_002859"
     CDS             3679..4218
                     /locus_tag="ACG8OL_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818756.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4215..4553
                     /locus_tag="ACG8OL_002860"
     CDS             4215..4553
                     /locus_tag="ACG8OL_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFS4818757.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1521 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa ataacggtgt
     3601 acatttttaa aaggagatgt ataatcgaga tttagaaagc gttttcctca aaatttaatg
     3661 aattaggaag tgattactat gaaaaaatta tttagcgcac tttttattct gttatttggc
     3721 tttattggta ttgggacttc tggaaatata gttaaagcta gcacgatggt tgaacaaaca
     3781 agtgaaatgg caaaaacttt tagtcaatta acaccagaag aacaaagtta cttcaaatcc
     3841 aaaggattta gtgataaaga tactttttac actcaaaaga tagagcctca agatccgaaa
     3901 acacgttttt ctgcaaatgt attgcggtta accgcatcta ctaaaaaaat tactaatacg
     3961 caggggcgga cagaatatat tataacaagt cccaattcgg cgatgtatcg tatttctaca
     4021 cgcttgagtt taggtagtgc aaaatcatat actcaaacag taaatctagc taattcttat
     4081 ggttatggtg gaggaatgta ttttacattt actgggaaaa gacaatatta cagtgtaaaa
     4141 ttagctatgc aagtttataa tacatttggt gtagggtcag tctcttgcac tgcgggagga
     4201 ttgacaatag gaaaatgata atttcttact atgtcttaaa tagaagtgat gatgaaggac
     4261 tcttcgtttt tcaatcatta catgaggata cgatgctaaa agagttttat aatcggatta
     4321 agaataaaca gatagaaaca aatttatatt ttttaagtga ctctatcttg ccttttatta
     4381 gcaaccctaa aattcttaac ttgttgcaga caaaagattt gaaaagttta ccgattattg
     4441 tcattgatga ggaaattgta aaatatggcg cttatctaag cgtagaagaa ttagaaaaat
     4501 taactaacat tggaatgagt attcaggagg ataatggggt acatggaaca tagaatgaat
     4561 atgcacagtt gagaacggaa tttgacattt
//
LOCUS       DAZUJO010000146         4573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000146 DAZUJO010000000
VERSION     DAZUJO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.154"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACG8OL_002861"
     CDS             complement(207..1169)
                     /locus_tag="ACG8OL_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4818758.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACG8OL_002862"
     CDS             complement(1171..2610)
                     /locus_tag="ACG8OL_002862"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS4818759.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..>4573
                     /locus_tag="ACG8OL_002863"
     CDS             2795..>4573
                     /locus_tag="ACG8OL_002863"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS4818760.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQK"
BASE COUNT         1443 a          793 c          751 g         1586 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttgtctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctatcc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtctattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaa
//
LOCUS       DAZUJO010000147         4486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000147 DAZUJO010000000
VERSION     DAZUJO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.155"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8OL_002864"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8OL_002864"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS4818761.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACG8OL_002865"
     CDS             complement(1036..1956)
                     /locus_tag="ACG8OL_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS4818762.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACG8OL_002866"
     CDS             2069..2821
                     /locus_tag="ACG8OL_002866"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS4818763.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="ACG8OL_002867"
     CDS             3685..4218
                     /locus_tag="ACG8OL_002867"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS4818764.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1490 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttacc
//
LOCUS       DAZUJO010000148         4433 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000148 DAZUJO010000000
VERSION     DAZUJO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.156"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG8OL_002868"
     CDS             complement(543..2048)
                     /locus_tag="ACG8OL_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS4818765.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG8OL_002869"
     CDS             complement(2045..2731)
                     /locus_tag="ACG8OL_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS4818766.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8OL_002870"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG8OL_002870"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS4818767.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAZUJO010000149         4333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000149 DAZUJO010000000
VERSION     DAZUJO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.157"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..564
                     /locus_tag="ACG8OL_002871"
     CDS             127..564
                     /locus_tag="ACG8OL_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818768.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            659..1543
                     /locus_tag="ACG8OL_002872"
     CDS             659..1543
                     /locus_tag="ACG8OL_002872"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS4818769.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1765..>4333
                     /locus_tag="ACG8OL_002873"
     CDS             1765..>4333
                     /locus_tag="ACG8OL_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS4818770.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
BASE COUNT         1417 a          725 c          966 g         1225 t
ORIGIN      
        1 attacttgat ttagttcttt ttattttaaa aatttttttt acttagaatc cagccaccct
       61 agatattttt ctgtataatc ttaaataaag aaaacgatat taaaaaatgc acgtagaagt
      121 agataaatgg agtggttttg caaatttctt ttatttctag aagttattca cgtatacgga
      181 tttgtttttc tcacaaataa aaaggcagga agtacaatgg atcatttgta tataaaatat
      241 attcatcaat tttccgtttt ggtattaggc acactaatta tagcggggat tgttgcattt
      301 attattcaag gtcgtgacat attaacagta catcaagagt ttttcagtta tgcatttggt
      361 attcctctga tcgctataag gtctgataac aaaaagtgga agaaacgcta ttacattctc
      421 ttgctatttt tgtgttttta tctgccacct aagattttta ctttattagg tatacaatgc
      481 ctatcatttt cttttatttc ctatatcatt gttttaattt tcctcagttt catagtatgg
      541 gtattttatc ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca tcacagccta
      601 atttttttct ttatgctatc gttatattaa agaaatcaat cagaagggaa agaaagtgat
      661 gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt gaacaagcaa aggaatttga
      721 gcaattagcg atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct tgcatacaaa
      781 aaaagcaggg gatgcaaaaa actttactcg cgaagcggct acggaagggt atcatagtgt
      841 gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg ctgaacaaga
      901 acatcggcca aatttcggat ttttcccatt gggtacggtg aatgacttgg ctcgagcatt
      961 agggataccg ctagaacctg aggaagcgat caatcatttt tcgattgaat cagtcaagcc
     1021 attggatatt ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa ttggctctat
     1081 ccctgaagca atcaatgatg tagatgcgga gaagaagacg aaatttggaa aactagctta
     1141 tttcatgtca ggaatcaaac agttagcttc cacacaatcc tactcgtttc acgtagaagt
     1201 tgatggaaag aaggaagaaa ttgagagcag tatcttgctg ataggactaa ctaactcagt
     1261 aggcggtttt gaaacattac ttccaaatgc aaaagtggat gatggtaagc tccatttggt
     1321 ttatttaaaa gattcttctt tgatcgatac gcttaaaaca ttgccagacc tattaaaagg
     1381 agtagatgaa tctacgaaaa atctagccta tcttacttgt gagcaattag ttgtgtcatt
     1441 agctgatgag gaagaattga cgacgaatgt tgatggagat gaaggggata aattacctgt
     1501 gacaatcaaa gtgttaccct cacatttaca ggtttattgt taaaatggag gttgtgaccg
     1561 aagagttgtc aggaggacat cggttgttcg gaataaggga ctgtgacaag acttttgtca
     1621 cagccttttt ttgtacaaaa aagaaatttt cacctaacag aaaaatttgc cataaaaata
     1681 catattttaa atatgaaagt acaattacaa ataaattaaa acgttaaaat aaaattgtca
     1741 aaacgtcaga gaggatggtg gagaatgaat caatatgtaa aaaagctgat tgcgtcgatt
     1801 ttgtttgtct tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaagc
     1861 gaatcaacgc aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcaatt
     1921 acttctgaaa ctacagaatc ccaaagtgag gcgcctactt ctaatacaag gcaagaagaa
     1981 aataagacaa gtgagacctc agaagtagct gaacaaacgt ctgatagcag tgaagaggta
     2041 caggttagag ttgtttcagc ggatgggtac agcgaagctg ccacaacaga agaactgacg
     2101 cagcttctcg cagacgaatc agtagctaag attcgtttaa ctcagccttt gactttagac
     2161 agggaattgg agatcaagcg agatatcgtt atcgattttg gcggacttgc tcataatttc
     2221 ggcacacatc acatctatat caatgagaca cctcagtcga tcgagtttca gaattttaaa
     2281 ggaacggcag ctcatccagg tgggttgata ccaaatatcg atggaaatgc cattatcatt
     2341 gcttatatgt ccgatttttg gggaaacaga tatcatttta cgggagaaat aaaatttacc
     2401 gggacacttg acttatatga tggttctaaa ttaggcctga tttacgcccc tcgtgcaact
     2461 gtcacattag atggtgtttc cggtgtattg gatgtccaac cagtgaaaga agggatgaat
     2521 gccgctccgg gtaaagctta tttttgtaga agctatcagc taaatgtaat caatggtagt
     2581 cagctttatg gaccatatct aggaaaattc tatggttttc tagattcagg tgatgagacg
     2641 ggcagtggaa aaacagcacc aggactccat atcttgagtg gttcaaaggt ctctcttgac
     2701 tatgatcggg cagatggcgt cagcgatgaa ggagaggctg tcgatacgct acccgggaat
     2761 gtcacattta aagtcagtgg ctcaggaagt gaattttcgg taaatacaaa gatcaatatt
     2821 accaacgaca acaatcgcgg aattatccaa atgagaggct caggatcaag agtcgctgtg
     2881 tcgaatgatg ggaaaatcac aatcaataca gcgactactg gcggatttcg cctgcaagga
     2941 gattctagtc aaatccatgt atcaagtggt ggacaaatct cggtcaaaat ggctggagat
     3001 ggcgaccaac ctggaaacaa tgggatgcgt tttgtaggga aaggactttc tttgatcgta
     3061 gaaggagccg gatcagtcat cgatattgat aagcaaagtg gtcgttctag cgcagttcgt
     3121 tttgagaacg gtacgcaaaa actaacagta actaatggag gctcattgaa ggtaagaaat
     3181 gcaggggatg gcaaatctta tgtgaacaga ctcaatcaag gaaaccaagc gatccaattt
     3241 tatgacgctt ctggttttct agaaagtcca gggtcagctg attttacggt tactggagaa
     3301 aaatcaaata tcgatatacg cgcagattcc ggagctaccg tagatacaac tggagatatt
     3361 gatttgaact ttatggctgg cgaaaaaact tatttagtca tggtcgggaa aactggggaa
     3421 gataagactg gcatcatcag tggggatgtg ttgaatgtag acttagaatc tcctacctat
     3481 ttcgatttcc aaaacaaacg gatcggaagc gagactaatg gcataggtgg ctgggtattt
     3541 caaggaaatg gcgattcttc tttgaagatc gctcattcag aaattgcttt atggcgaaag
     3601 gcaggagtaa cgttcgataa ctttgaaaaa gatccggatt atttttctga tatgacagac
     3661 ttatcgatca atggaaccga cttaggtaat tttgtatcaa gccctagtgc aggtttaaca
     3721 aatgaatttg ctcagacagg tgccggaatg aaaaattata atcgaatcag tgccaataac
     3781 cagcttccga tgattgaatc cttaagagta ccaacgaatg cagataaaaa aatctatggg
     3841 catgtcgttg ttcccgaagg tgtcgaggca aagccaagag atgcttggac aggcgaagta
     3901 gaagtgactt tacgtattac ttatgcagat acgacgaaat cacctgtaga aattactgct
     3961 ctcacacaag gacaaacaga tgaaaatgaa gggacaggat ataatccgtg gggagaaggc
     4021 gaacaaggcg gtttgtttga ggttcaagta cccaacgaag aaagactaac aacgggggat
     4081 caagtgcaaa tagttgctgc taaaaaagta agaggcggcc aagaagtgac agaactggac
     4141 tctgccaaaa cgacagtcga tgtgattcct cctgagcctg ctaaattaag taccacattg
     4201 atcaatgcag cttctcgctc aatcacaggt acaggcgaag caggagcttt ggtttcattg
     4261 aaaaaaaatc aagctgtttt gtccgaaaca actattgatg atacaggtaa atttgttctt
     4321 tctatcccag aaa
//
LOCUS       DAZUJO010000150         4069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000150 DAZUJO010000000
VERSION     DAZUJO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.158"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACG8OL_002874"
     CDS             85..1434
                     /locus_tag="ACG8OL_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS4818771.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACG8OL_002875"
     CDS             complement(1562..2152)
                     /locus_tag="ACG8OL_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS4818772.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG8OL_002876"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG8OL_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS4818773.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1175 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DAZUJO010000151         3995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000151 DAZUJO010000000
VERSION     DAZUJO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.159"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2690)
                     /gene="brxC"
                     /locus_tag="ACG8OL_002877"
     CDS             complement(<1..2690)
                     /gene="brxC"
                     /locus_tag="ACG8OL_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFS4818774.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSR"
     gene            complement(2715..3290)
                     /locus_tag="ACG8OL_002878"
     CDS             complement(2715..3290)
                     /locus_tag="ACG8OL_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFS4818775.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(3290..3889)
                     /locus_tag="ACG8OL_002879"
     CDS             complement(3290..3889)
                     /locus_tag="ACG8OL_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFS4818776.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1193 a          771 c          543 g         1488 t
ORIGIN      
        1 cgactttgaa acagttctct accgatttcg tcaaccaatt tttttgcttt tggcgaaatc
       61 accgcgcttg gttcaatttt tatcttttca ctttctcgtc gtttttgaat aatcattaca
      121 atactatttg gatcatccat ccgtgaaatt tgtacattgt tataaagtaa atcaatttta
      181 ccagacatga aaagtttagc gatgcaccat tccacatcaa gttctgtgaa tccatacggt
      241 tgttttgttt caaaacgatc acgaattgtt ttcatcgtaa tcgttaggtg atttctcttt
      301 tgatcattga taaaattgaa aatttcctga actgcttgct catttgatgg atcttcaata
      361 atttcatttt tgctttcttt cactaaatat aagatatctt ctttttcttt aggcgcatca
      421 atttggttta atttatggta tacttcttca gccattttct gaagtgctga ttggaatttt
      481 gctttaaagt cggagcctgt cagttgaatt tctgcaccgt tatagaaaaa ttttgcagat
      541 ttcaacagtt catttaattg atcgttgata cttgcactta gttcctgtaa ttcaccagat
      601 ttttccgaac gaatcgcgtc aaagttcttt actacactcg aataagtcgt tgttaaataa
      661 agattcattc tagcatacat tgctatatca tctttaaatt ctttatctga tggcaattct
      721 acgaataata agttacggga actcttaagc gcaagttctg cctcttgacc tgctaaatca
      781 gagtatggtg aaataaaatt aactgtcatt tcagcatttc cagcttgctt gtatggacgt
      841 tgatctaaca cttgtgcata agtaaacgta taccggtttt taaatttatt tccacctgac
      901 atgcctaatt tttcataggc aaaactattt cggtctagaa cgccgtcaaa aattcgttta
      961 gatagttcta cgatcaaatc actatctgta atattttgat ttttaatctc acgagtaatt
     1021 tcctgttctt catcagtttg gaaaatataa ctatctaagt tcttccgcac aaagttttcc
     1081 ttaattaaaa ggtccaatgc ttcttctact cgttttttag tttccaggcg gtcttcgttg
     1141 atatttctta tcatcaagct cgtaatattt tcaacatttg gtttgatggc ttgatcaaca
     1201 tatttaatca tgaataaaat tcgtaagaca tttacgttaa agttatctat atcagttggt
     1261 ctttgaatat gagaattatt tgtagcttgt gtaatcactc ggctaattgc tgtatcaatc
     1321 caacgttcta acgagtaata aaactgatct aaagaaacca aactggttgt atcattttcc
     1381 ttatttcgtt cagcggcttc tttaaacatt gctagtaatg aacgttcacc agacgataag
     1441 tttgatcctt gatagctgtg attacgaatc tcggttagaa tttgccccaa taaattgaat
     1501 tgatagggaa taaatggata aacttctgcg taattttcag catcttggaa gagctttaat
     1561 tcaggactat caacaaagag taatttattt ttcaattctg tttgaacagt ttcaaattgt
     1621 aaggctaaag aatcattggc aaactctttt ttcttcaaaa tacgttcttt aattacttca
     1681 tcggcatttg ctgaagataa actcacagtt gtggcaaaac gatccttgat cttagaaaaa
     1741 tcattatcag aattgttatc aaagtttgtt acgtgatcaa tcgcttcctg acttgtcact
     1801 aatacccaag ccttaccttc acaaattcga cccaaatctt ccacaacggt ttgtaactgc
     1861 aaagtcaact cacgattacc gccgatatat tgtccgacct catcgactag aaaggcaata
     1921 tgatggtctg cttctttgga atcaagatag cttttaacta gatttgcaaa gtcatcaatg
     1981 cttattaaat aatttcctga tgctttttct aaaaaattat caatagcggc ttcactcata
     2041 aaatcgattt taagtgccgc ttctttgata tcatcttgga tccaatcaaa ctcatggcgc
     2101 tcttcttgcc attttgcccc attaatctct tcaaaagcag ctttgaatgc atcaaacttt
     2161 tcttctttaa ttaaccgacg ttcaaaatct gctagatgtg gaatcgtgct taatccttgc
     2221 atttcgttaa atacctttaa aaaaacattc acgattccgt ttttgtcttt tttaccacct
     2281 gcatcacttt ttgaatcaat attaaacaga ataacatctg tatttatttg tgagcaccgt
     2341 tccatcgttg ccaaagtcag tggatctttg attttcccat cttctttgaa gtaatcaatg
     2401 gctttcttgt catttatttc tcgatttgcg agcaagtaag aaataatttt taagaaatga
     2461 gatttacctg aaccaaagaa cccctgaatc caaacaccca ttttatctgt tggtgtattg
     2521 atactccgtt ggtaggcatc aaaaattgtt ttaaagtgtt tttgtagttc tttcgtaacc
     2581 acgtattctt ctaattcttg ttgaacattt tcatcctttg cttgaccgac cttaataact
     2641 ccttgaatat ttcggtcaat tggttttgca aaaatatctt taattagcat ttaatctcct
     2701 accttccatc ccaactaaat tctaaatgct cgataataat tatcatcttt attggtatta
     2761 agaattgtca aacttaattc atcatattcc cctggataca tcatcacaac tggtacgcga
     2821 tcaaatacct gatgcatttt atttaaaata gagtgcgctc taattaatgg atataattcg
     2881 ccaattcctg tcaggaacac aactgatttt tctggtgtat gggcatccac atattcaaaa
     2941 aatacatttt cttcctcagg taaatccatt ttcaataaac gaccaatgct ttccactaat
     3001 ttttgtaaac cacgtcgttc ttccatctta atcgcttttt cagtgaaacc gcgtttttct
     3061 agatactcta acataatcac atagagatta taaactttca cttcataatt atcaccaaca
     3121 gatgttccag atatatcatt aatataatct cttacaatta actcgtcttt cggatcgtag
     3181 tcaaagatat aatagcctac ttcattacct aaaccacgat tcaataaaaa atcttctttt
     3241 tgaattactt cattcaactt ttccaaatgc tcttgaatcg ataatgacat tataaaattc
     3301 ctcccagtgc tagtacaata tctttacgat tcttgtcaac taaccattga atcaatcgtt
     3361 tatctaataa tatcggagtc attcgtccat attctttttc ccctctgact aatcctgttt
     3421 caattagatt cgtttgataa gcggcaataa gccgtttaat cgtataatct ttccattttg
     3481 aaatatcttc tgaacttctc cgcttctctg taaaaaaaga acggtaatca actggtgtta
     3541 attgcaaatt tcctttacgt agattttcag catatacctc agtcataaat tcattaaaaa
     3601 gatcatcaat cataaggatg gaaatcaaat tcactaactt ttgattatct atatttaact
     3661 gattgaaaag ttgcaatcca gcttcatcca gttgactaac tctccttttc acgttctgat
     3721 agatttcttt ttgacggtta ctgctcactg caaaaaaaat attttcatgt tcatttttct
     3781 ctttaatttc tatatctgta tatccttcta gtcgtaattg aatatataac ttgaactctg
     3841 aaaaccaaaa gcgtttagaa actagaccag cagaatattt tttattcaat agattgcctc
     3901 ctaatcatct ataattcaat aagtaaacaa gtgttttttg ctcatcgtta gcattcattt
     3961 tttagtatct actatacaat acatatttat tatag
//
LOCUS       DAZUJO010000152         3984 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000152 DAZUJO010000000
VERSION     DAZUJO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.160"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..649
                     /locus_tag="ACG8OL_002880"
     CDS             <1..649
                     /locus_tag="ACG8OL_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS4818777.1"
                     /translation="ILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVF
                     EKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIE
                     HLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKI
                     STAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            746..1099
                     /locus_tag="ACG8OL_002881"
     CDS             746..1099
                     /locus_tag="ACG8OL_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818778.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSESN"
     gene            1716..>3984
                     /gene="esp"
                     /locus_tag="ACG8OL_002882"
     CDS             1716..>3984
                     /gene="esp"
                     /locus_tag="ACG8OL_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS4818779.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
BASE COUNT         1456 a          543 c          760 g         1225 t
ORIGIN      
        1 aatactggaa tatgttaaaa atggagatat tcgaaattta gaaaacatgg tttttaatct
       61 gagtaatgga ataattccaa gtgttagtgg agatacgatt cggtctgaaa aaaattattc
      121 tatcattgtt tttgaaaagt tagcacaaac gtctataaca ttaggaatgg atataataga
      181 agcgtatcaa agtagagatg ctttgataca agaaaacgaa ttagctgtat cgttacctga
      241 ggtcttaaaa gtgagggatt ctgggattgt ttattatact aaggaaatcg gaaaaactaa
      301 gatagagcat ctttctccct taatttcttc tgtcgttcaa ttcatcggct taaatatcta
      361 taaaagaatt actgtaaagg aaatagctaa ttatttttct gtaagtgaaa ctaaattaag
      421 agaaagcttt aagaatgaaa tgcatattac gatttataat tatatctcta agcgaaaaat
      481 ttctacggct aaaataatgt tgaaatcaaa ccatactatc agtgaagttt ctttaggttt
      541 aggattttca gattcttcac acttttcgag agtattcaaa aagtacgcag gtgtatctcc
      601 taagcaatat caattaggtt tagtggataa tttcaacaac attagctgag aggttttatt
      661 ttattatgag actatatcat ttacgtagtt tggaggagtt gaggaaattg gattttaaca
      721 aaaaattttt tgggaaattt ctgatttgag ttgtttcgta gaatctgagc gtctaagtaa
      781 tggtaataat agtgtgttaa atgaatatga caaagtagta attaataaag taaaaagttt
      841 atgtgaagaa gctagagaat ctatcagtca tattaatggt agcaatttta agcaaacatt
      901 atttaatctg attaagctag atgctaaaat atcaagttat ttattctttt taatacatga
      961 tgaatttatg gattatcaaa aattggatca gttaattgaa agagaaagct gggaagatta
     1021 ttacgtgggt ctaacttttt cagaaaagtt aaacaactac aagttaatag attataagta
     1081 tttgagtgaa agcaattaat tattcatgta gagtactaga cactttaaat atggacaatt
     1141 caattataaa gaaaaagaat caataattta atgaggctgt gacaaatgtc attttttgtt
     1201 tcgctaaaaa cgagtgaaaa aatgttgata actccaatca ttcgtttaag attctaggaa
     1261 tcattcagga atgctgtgaa accgatactt tataattata aagaaggtga tttcaaattc
     1321 cgaagaagga atttgaagtc acctttttac ttatgtcaca gtctcattca gtaatgcggg
     1381 ataaggctcc ttattccgct atttgaatac tctgaagttt aatctaagaa agagtaatat
     1441 tttgaaaaca tgcttcatcg catagaaatg aacttaattt cagaaagaat acatacatat
     1501 ctttttatat gttttcagct gctgaatggt taaaaagcac aaatatccga aaaaaacatg
     1561 aagaaatgta ctttttttgt tgtaatgtgc atgaaattct ttaagtaaac tagttaacat
     1621 aagtttagta agattcctat tctgtgaata atacgtatca gatttatctt ttattcttac
     1681 taagaaaata actattaagg gagttgattt gaataatggt tagcaagaat aataagagag
     1741 tatttctaga aaaaacaaag aaacgagtat tgaagtacag tatcaaaaaa ttaagtgttg
     1801 gggttgcatc tgttttggtt ggtgttggtc ttgtcttggg aacaactgaa ctagttcaag
     1861 cacaagatga aatttctcca agcaccccac ttgaaacagc gataagctcc gtacaagtag
     1921 gtgacaaagt agcttcgggg aatacttttc aggagaatcc tggttatacg aaaaattata
     1981 acttttcgga tttacaattt agtcctcaag aactaactgg agacacacta aaaggcaata
     2041 caattggatt tgaagtttac ggaaaacata atattgcagc atcaactaaa aactgggaaa
     2101 tccgtcttca attagatgaa cgattagcta aatatgtaga aaaaattcag gtcgatccaa
     2161 agaaaggaat tggatcttct agacggacct ttgtaagaat taatgattcg ctgggtagac
     2221 ctacaaatat ttggaaggtc aattatattc gtgcaagtga tggattattt gctggggctg
     2281 aaacaactga tacacaaact gctcctaatg gggtaattac gtttgaaaag agcttagatg
     2341 aaatttttaa agagataggc atagacaatc tcaagaccga tcgtttaatg tatcgaatct
     2401 acttggtaag tcatcaagac gatgataaaa ttgtaccagg tattgatagt acgggctact
     2461 ttttaacaga ttcagatgat ttctataata gcttagatgt atctgaaaat aatcctgacc
     2521 agtttaaaca tggatcagta agtgcaaaat atgaggaacc taatactcaa acaaaagatg
     2581 gttcaggatc cacaggtgct aatggagcta taatcttaga tcataagtta acgaaaaatt
     2641 ataatttctc atattcagct tccgcaaagg gcacgccttg gtatgctaat tacaaaattg
     2701 atgaacgatt agttccatat gtagcgggta tacagatgca catggttcag gctgacaaag
     2761 taacatatga tgtttctttt gaatcaggga aaaaagtggc tgatttagca attgaacggc
     2821 gcaaagatca tgaaaattat ggtgtaggtt caattactga taatgatctt accaaactta
     2881 ttgattttgc taatgcaagt ccacgtccag tcgttattcg atatgtttta caattgacta
     2941 agccattaga tgaaatctta gaagatatga aagcaacagc acaagttgaa gaaaataaac
     3001 catttggtga agatttcatc tttgattctt ggttgtcgga tacgaataaa aaattaatcc
     3061 agaacactta tggaacaggt tattattatt tgcaagatat tgatggtgat ggaaaccctg
     3121 acgataaaga agagagcgga gacacgaatc catatatcgg gaaacctgaa ttagaagaag
     3181 tatatgatgt tgacacaaca gttaagggga aagtattcat ccacgagtta gcgggaacag
     3241 gtcacaaagc ccaacttgtt gataaagaag gtactgtatt agcagaaaaa actatcgctc
     3301 caaatgaaaa agatggggct ccaatttcag atactgtaga atttgaattt acgggtgtag
     3361 attcaagtaa attaatcgcg aaagatgaat taaaaatcca aatcgtttct ccaggttttg
     3421 ataaaccaga agaaggttca accgttatta aggaatcacc aaaagcggtt gataaacaaa
     3481 ccgtggtagt tggatttaaa ccagatgcta aagaatcaat tcggaataat aaaaacttac
     3541 ctgaagatgc agagtattca tggaaaacag agcctgatac ttctaacgtt actgatagta
     3601 cgaaaggtat tgtaactgtt aagatcggaa atcgaacttt cgacgtggat gtagagtttg
     3661 ctgtaaaagc ttctcaagct atggaaaatg atgcaacata cgtacctata acaacaactc
     3721 cagaaacgac agttcaaagt ggaaaaccta catttgataa accagatgtt cctttagcta
     3781 aagatgcctt ttcaatttta gatgtctata ataaggactt cggtaatgca agtgttgatg
     3841 caaatactgg tattgttaca ttcactccag ctaaaggtgt aggagaatcg gagccgatta
     3901 ctggaacaat tcctattaaa attgtttacc aagatggttc tgtaggcacg accgatttag
     3961 cagtaactgt aagtaaagat attt
//
LOCUS       DAZUJO010000153         3894 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000153 DAZUJO010000000
VERSION     DAZUJO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.161"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..391
                     /locus_tag="ACG8OL_002883"
     CDS             <1..391
                     /locus_tag="ACG8OL_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818780.1"
                     /translation="LLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLW
                     LLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFI
                     GILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(517..1194)
                     /gene="nth"
                     /locus_tag="ACG8OL_002884"
     CDS             complement(517..1194)
                     /gene="nth"
                     /locus_tag="ACG8OL_002884"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS4818781.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1201..1899)
                     /locus_tag="ACG8OL_002885"
     CDS             complement(1201..1899)
                     /locus_tag="ACG8OL_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS4818782.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2167..3351
                     /locus_tag="ACG8OL_002886"
     CDS             2167..3351
                     /locus_tag="ACG8OL_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS4818783.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3357..3800)
                     /locus_tag="ACG8OL_002887"
     CDS             complement(3357..3800)
                     /locus_tag="ACG8OL_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS4818784.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1011 a          767 c          637 g         1479 t
ORIGIN      
        1 tttattatta aataaattta ctttaattgc tcctatcctt gcatttatta ttttttctgt
       61 acttttaagc actactctta aaggaaaatt tatggaaaga agctctcatg caatgattct
      121 cactttttta tggttgttgt tcaccagaat ctatattttt ctgatgagcc tgaaaccgcc
      181 caaatccatt agtttatgtt tagttataaa tggtattttt ttattatcac ttatcatttt
      241 tattacacct ttagatagat acgtcactta tttatataat cctttggaat actggactta
      301 ttttataggt atattggaag gcattatttt ctacatcggg tattttccca ataaaaataa
      361 caacttttat ttttatcgca ataaattgta ataagttacc atcctagcta ctgagtatgt
      421 acatttactt tttcatgcaa aaaagataaa cgaaacagct tttctccata ataacaagat
      481 aattcataca tttatttgca attaaagaaa ccacgtctac aggcgtggtt tcttttttag
      541 cgccttttct tttagacgca tccgattttt cccatcttga catattgata gcaaaggaca
      601 tacctcgcac ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa tcagtgtatg
      661 atgtgtcttt acccataatt cttgtggtac tttccgcatc agtgtttctt ctacttccat
      721 gaccgtagca tctagtttgc agatcctcag gcgttttgag acacgttcca cgtgggtatc
      781 tactgcgatt gctggtatcc cgaatgcatc ccccaatacg acatttgctg tctttctgcc
      841 tacaccagga agagacatca attcttctct tgacgttggc acttgcccat cgaaacgttc
      901 aatcagctgc tgtgcacacg ctttgatgtt ttttgcttta tttcgataca aaccaatcgt
      961 ttttatttta agaatgattt catcgattga agcttctgct aacgcatcgg gtgtcggaaa
     1021 agaggcaaac aaatctggcg ttgctttatt cactgataca tctgtcgcct gggcacttaa
     1081 gatcactgca atcaataact caaatggatt attgtgcttg agttctccat gtgcttcggg
     1141 aaacattcca tacatcgttt ctagagcttc cattgttttt tgtttgctta acatattccg
     1201 ctactcctcc aaccaatttt tcagtgagac tttcggcaat tcttcttgtt gagctgactg
     1261 ttctacttct ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt ccacttgttg
     1321 ttttgtacgt agattttttc tttcccacga tagcaaaatg cggtcaacgt aattaaaact
     1381 ataagcctga tttaatacgg cttcccgcag tgctagcttc aaaatttccg gctgataatg
     1441 gtcttcttct agccattgtc cgatccgctg gaactcgatc gaagaaagcg ggcgtccaaa
     1501 ttcttgttca aataattgat acactgactg gatttctttt tcttgtgttt tttcttcgta
     1561 ttttttttct tgtgttttta aatactcacc taatagatca tagatggggt aaagattata
     1621 tcgatctgct tttttaccgt tgacaatcgt cgtttctatc ttgataaatc catttgttac
     1681 gagacgatcc agtatttgaa aaatctgatc ttgtttgatt cccatatcca tagcaatcat
     1741 ctgcaaatca gggaaagtat ctccttcaag ctgtgccata tgcaactgta gaacaaataa
     1801 aaattcttca gattgtagac cgatcctatg ataattttta agtaaaagat tagatatgac
     1861 tgtttgtcca gcgcttaagt aattttctaa attcaacatg atttctcctc cgctcaatca
     1921 cagtatacgt aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt tacttcactg
     1981 atcggtcaga tttacaatgt cgtctacttg atttttgttt gttcatgttt ttgatgcgta
     2041 tcttcacttt tcggaataaa taaattaagt agatttcgct gttccttaga atgttgacaa
     2101 ggaaacaaaa ttactataca ctatttttgt ttccaagtta acattattgc ataaaggagt
     2161 ttttacatgt acagtttcac aaataaacat tctaaagttt ttggatacgt ctctgtctta
     2221 ttcactggat taggatttac tatcgtcagt cctgtacttc cgtttttaac gctgccttat
     2281 tctcactcca tacatcagca agctttctat atcactttgc tcatgtctgt ttatgctcta
     2341 gctgccttct tatctgcccc cattctcggt agcttaagtg atcattttgg gcgtcatccg
     2401 atccttatta tcagtttatt aggatcaagt atcggatatt tgctttttgg tttaggaaat
     2461 tcgatatgga tgttgttcct tggtcgtatc attgaaggat tgacggcagg agaaatctcc
     2521 accttatatg cttactttgc cgacatcacg gaacctaatg aaagaaccaa agtttttgga
     2581 tggatgggtg ctttagtcgg tataggaaca acgctgggtc ctattatagg gggtctatta
     2641 gctgagttgg ggaattctgt tcctatattt attggagcac tctttacttt cttaaatgcc
     2701 gtttatggct acacatttat gctagaaagc cttccaataa aaaaacgttc agtagattta
     2761 agtttctccc atgtaagacc ttttcaccaa ttacaacaat tattcaagat ttcatccgtt
     2821 attcctttat tgacagcagg ttttgctgtt tggttggcag ctggctctct tcaatctatc
     2881 ttttctcaat tttcgattga cgcctttcag tggaaggctg ggttggttgg tttaagcttc
     2941 tctttgattg gaatcttgga tactgtctcg caattgttca ttatgcctcg attgctcaaa
     3001 aaattttcag aacagcagat cacaagaata gggatgttca gtgaaatcct cgcctatcta
     3061 ttcatcacat tatctggaat tcttctactg ccgattttgt ttttatttgg gattatctgt
     3121 tatggtttcg gtgattctat ttttactcct gtattcaatg gccaactatc caattctgtc
     3181 tcagataacc agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc
     3241 gtcatcggcc cgctaatcgc aggacagctt tatgctgcag caccatttct tccgacggta
     3301 ttcggatttt tgcttatgat ttttgcctat tatcactaca ataaaaaata gtagcttcat
     3361 ttgctacttc gtataaattc tctgtatcga ccgataaaac gaaatactgt gtcaaattct
     3421 tcttttgaca tattttcaaa tacagtagca tctcgctgta cccatttttg atggagttgc
     3481 tcgtgtctat tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta
     3541 ttctcttcgc tttgataagt gtttatgatc cctctagcta tcagtttttt tgttagctta
     3601 ctgatggctc ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg
     3661 attttcccga tatgttcaat cacttctagt tcatttagag taaatcctgc aaattctttt
     3721 tccatttctg gtttattacg atgaataatc tcattagcta aatccagata ttcttctatt
     3781 agcagttgtc ttttatccat ttctacacct tctttttctt tcattatatt gtttttttgt
     3841 ttcctattca acaaagataa aaatattttg taggaaaacc caatctgaaa ccaa
//
LOCUS       DAZUJO010000154         3882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000154 DAZUJO010000000
VERSION     DAZUJO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.162"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1178)
                     /gene="xerS"
                     /locus_tag="ACG8OL_002888"
     CDS             complement(93..1178)
                     /gene="xerS"
                     /locus_tag="ACG8OL_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS4818785.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1527..1722
                     /locus_tag="ACG8OL_002889"
                     /pseudo
     CDS             1527..1722
                     /locus_tag="ACG8OL_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3254..3772)
                     /locus_tag="ACG8OL_002890"
     CDS             complement(3254..3772)
                     /locus_tag="ACG8OL_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS4818786.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1254 a          634 c          585 g         1409 t
ORIGIN      
        1 aatataaaaa tgataatgaa gcttgccttt aaagttgggc ttttatctaa ttttagagac
       61 aggcttcatt attatactat tttttactac atttacaaag cgttcaaagc atcaattgta
      121 gctgcattgt ctatatgagt atataaagtt gttgtatttg tacttgtatg tcccaattgt
      181 tgggcagtta ataccaatga atttgtctgt tgatagagtc tagtagccaa agtatgacgt
      241 aatttatgtg gactgacttg gactctgaat ccttttgaat attttgctac catccgttcg
      301 attgcaatac cggatatacg cttggcttct ccttgataaa ttgctagaaa taaagctttt
      361 tcgttttctg ttactttgta tcgttctttc cgaatagcta gataattagc taaatattcc
      421 attgcaaatg ctgctatatt tactgcgtct ctcttatttc cttttctagt tactaccaca
      481 ttattctttt ctaaagatag gtcctctaaa ttaatattta caacctcgga gagtcttaat
      541 ccagatccta aaatcaatgc attgatcgcc aggtctcttt ctacatctcg ttgataatag
      601 atcaaagctc gtttgctcaa agttttttta tattcattgg caatataatc tagataattt
      661 tgtgtatctt cgtctagaaa taattttttc tcgattgcgt ttgcacgggc agctaacgta
      721 gttttatctg tgatagttgc tacttgatcc ataggattat tttctaagta tggttttcct
      781 aaaggattat tggaactttt cgaaagatat ttaaatagaa cctttaatgc tgtgatattt
      841 ctattgatag tagaagtaga tagacctaag ttattattat ctttgagctt agtttttgtt
      901 gtctcttttt ttacattttc ctttgtgcga cgtttcaaat gagatttgta caagtttaga
      961 tcataggcag ttaactgtct tatcgaggtg attgaagtat cgggaacgat agactcactc
     1021 agaagccatt cataaaagag cttataattt tttagatatt ctaaagtagt tcgaggtgat
     1081 tgatctttat cctcacgatc ttgaacaaaa tcttttatat aatcgggcat atgaagtatg
     1141 attgtatcta ttttttgata tatttctgta tttttcatgt tattcatcct ttcgataagt
     1201 aggggagagg agtatctcta taattggact ataccaatta tagagtgttt tgtcaattgg
     1261 tatactctga aatttattaa tttatttcaa aaaagcagat aaataggtat agatttaata
     1321 atttaaaata aatggtatac atgaatagga aaggaaacag aaggaggcaa tcttaatttt
     1381 ttaaattgtc taaattatat agaaagaaca atctttctat ataattagac ataagagaga
     1441 ctacggttga tttgatatta acaagcgtat atgaatgtat atacaaataa aaaaggggag
     1501 ggagaagtta aagaaaattc agagaagtgg ggcactcagt agtattaaca atttaaaagg
     1561 aatttcttaa agaaaatggg ttgcatcctg gagatacaat ttttttagat aaagaaaaaa
     1621 ttcaagcaac aattcaaaaa aatcttaact gctgagattg atgtagcaat atctcaatca
     1681 tcaacaaagt acgatcctgc ctttcaaaag cttattgata aatgtttatt cattataaaa
     1741 ataaaagaaa aatttatgtc agcttttttc atcaaagtgg taatgataat tcgtctatta
     1801 attgtatttt ttaggaaata tggtataatt gttttaacga aaaggtaatt catgatacac
     1861 caaaaaccac tagctggcgg gctagtggtt ttcttttacg ttctattcag attaacgggt
     1921 aggcttactg tcttttttgg caataatttt gcaatcaagc catttacaga tgtaatgcgc
     1981 ggatacttct gctaagattg caaaaaacat atcgaaaagg taattcataa tacaacctcc
     2041 tccccttgcc ataataggcc ggggtagcag acagcataaa tattatacca taactgaaaa
     2101 tttcatttta gtacatttca atatattgat gaaccagcac aataaaacat gccatgacta
     2161 acagaaaaaa acttattaaa ccagatataa cttgtaaaca tccaactata tagaaaacgt
     2221 aaattttcta tatagttgga ttaagataac gactatttat tttatcatat ttttactata
     2281 ccctatactt ttacttggta tatttatgaa taatcgcaaa tttaccccta tactactagt
     2341 ttgtaagtta gattaataaa aatactaaag ttatttgttc ttttattaaa tacacctaca
     2401 acttataaat tgatggtatt tagataacac gaaagaataa aaataacagc tgatagtaaa
     2461 gtttgttgac gattgctact caatcgaaag tgagttagtg cgtatgaact tgcttctgaa
     2521 aacttatgaa atgaaagacc ttttacatct tgcagaaatc gttactggga ttaaactaag
     2581 caataaaaac taatgttccc ctcattcttt gaacttctac tatttgattt atttattctg
     2641 ataaatgacg atttcacctt caggatcttc aaatcccgta tagaatttat ttaagatctc
     2701 ttcagtatca gtaataggat aggcgatagg ccacataccc tttgatggtt agaataaact
     2761 gctcaaaatc tgtatcgtat gttggcttta gcatattaac aaaggctatc tccaaattat
     2821 aaaccataag cgttgaatcg ttcttaacaa tttcaattct tcctaaccca aagtcagaag
     2881 atataattac cggacttgcc ttacccttca tgtgggttgt tgagattcca tatttaaagg
     2941 gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct
     3001 gctagtctcc taagtccctt tgtagcggat atggcaccat aatctcattt tatcgaagtg
     3061 agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc accctctacg
     3121 gcattctagg ggagtgcact tgcattcacc caataaaaac cggcagttag caaagaactt
     3181 atgtagtcgt atgttcttac tgttaacata caagaggtct cctttccaca attgcttagc
     3241 agtaaacggg aatttacaaa gctaatctct tccattttct tatctttgtt ctaaaagtcc
     3301 caaatggagc aactgtattt acatgaataa atttatatac ttcccataca gccgttttgg
     3361 ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag atcttcatca ctcattgaat
     3421 caatcatcat gttaatatca tcaatattac gattgagaat ttcctctaat tctttcaatg
     3481 gtagatgtgc ataggtattt gtaaaccact tatataactc tcctaactga ttccatttaa
     3541 attgatcaga aggcgtttta acagataatc catttctttc atcattttcc cattttagaa
     3601 ttaaattcgt ccaaccaact tgatatgcta aattttcagc aggtgttcta tccacttcct
     3661 caactctttt atccttcaat tcttcaggaa tagtttcaaa ttctaaaata tatttagtat
     3721 aagtcctatt tatttctttt tttaattctt ctttattttt atatgttctc attatattac
     3781 ctccataaat tcctaattgt ctcctttaat tcatctataa tttcatccac gatattttaa
     3841 tccgttctac aatgcatatc tgatatgcat tgactgtcta ac
//
LOCUS       DAZUJO010000155         3850 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000155 DAZUJO010000000
VERSION     DAZUJO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.163"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1155)
                     /locus_tag="ACG8OL_002891"
     CDS             complement(1..1155)
                     /locus_tag="ACG8OL_002891"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS4818787.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1340..3781
                     /locus_tag="ACG8OL_002892"
     CDS             1340..3781
                     /locus_tag="ACG8OL_002892"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS4818788.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         1231 a          721 c          840 g         1058 t
ORIGIN      
        1 ttatattttg tagtaacgaa tatcattttc gatctttgta atcgaaaaag gtacatctga
       61 agtatattct gcacattctc cgtgttcagg aagcgtctca ctgaaaatcg tttcatattc
      121 gtcaacagtg atccgtgttc gagaatctag catctcttga tgagattgtg cgaagagata
      181 ttcttgatag ttttcttcca gataacctgt aaaaaattca cttactgcac cagaaccgta
      241 gctgaataga ccgatgcgat ctcctggctg tagtgatttc gaattttcca gtagagaagt
      301 tagccccagg tataatgaac cagtgtaaag attaccgatt cgtcggctgt aacggatgct
      361 ttcttcatag cgagccataa gacgttcctg attttcttcg tctgtttggt ctaatacgct
      421 ttgcaatgcc ttttttccca tcttagtata cggaatatgg aaagcaatcg cctgataatc
      481 ttcgagtcct cgacccgaca attctttatg tcgattccaa actttttgga atgattcgat
      541 atacgtagaa ttagataaag gaccatcaac aacaggaaat tcgctataat ctggacgcca
      601 gaaatcatag atatcttctg tcagaaatac gctgtcgtct tcaatcgata aaatacgcgg
      661 gttttgagtg atcatcatcg caacagcacc gacaccttgc gtcacttcac caccgcttgc
      721 caagctgtaa cgagcaatat cacttgctat gactagtact tttcgttctg gatgattttt
      781 gacatattct ttcgccatgt gcaaagctgc ggtgccaccg tagcaagcct cttttacttc
      841 aaaagaacga gcaaaagatt ggattttcag taaatggtgg ataattaccg cactcgcttt
      901 tgaatgatca atcccggatt ctgttccgac gatgaccatg tcgattagct cacggtcttt
      961 ttctgttacg attttgctgg cagcatttgc tcctagtgta atgatgtctt catttgcacg
     1021 attgactgcc atttggtctt gaccaatccc tatatggtat tttgcaggat catccccacg
     1081 gctttctgcc aactctgtca tatctaaata taaattagga ataaaaaagg aaagacgatc
     1141 aatccctatt ttcatttcaa cacctcttta cttatgataa aaaacttact ttactaaggt
     1201 atcataaaaa gattaaaaga aatataaaaa agagaatatt taaggattgt taaaatttaa
     1261 tcttagaaaa aaataactgg aacaagagct atttgttttt acagcaaaga atggtatatt
     1321 tttgtaggag gtggcttact tgaaagaagt cgttatgata gatgctgcaa gaacgccgat
     1381 aggaaagtat cgcggaagtt taagtccatt tactgcagtt gaattaggca cacttgtgac
     1441 taaaggatta ttagataaaa caaaactgaa aaaagataaa atcgatcaag taatatttgg
     1501 aaatgtcctg caagctggta atggtcaaaa tgttgccaga cagattgctt tgaacagcgg
     1561 tctccctgta gatgtcccag caatgacgat caatgaagtt tgcggatctg gtatgaaagc
     1621 agtgatctta gcacgccagc tgatccaatt aggtgaagca gaacttgtga tagccggcgg
     1681 aacagagagt atgtctcaag cacctatgct gaaacagtat cagtcagaaa caaatgaata
     1741 tggtgaacca atttccagta tggtcaacga cggattgact gacgcatttt caaatgcaca
     1801 tatgggatta accgcagaga atgttgcaac acaattttct gtgagcagag aagagcagga
     1861 tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct gtcgaagccg gtgtattttc
     1921 tgaggagatc atcccagtca agatttctga tgaagacgtg ttatctgagg atgaagcagt
     1981 tcgtggaaat agtacattgg aaaaactggg cacgttacgt acagtattct cagaagaagg
     2041 aactgtaaca gcaggaaatg cttccccgtt gaatgacggt gcctctgtgg tgatccttgc
     2101 atccaaagaa tacgcagaaa ataataatct gccttattta gcaaccatca aagaagtagc
     2161 ggaggtcggt attgacccaa gtatcatggg aatcgctcca atcaaagcca ttcaaaaatt
     2221 gacagataga tcaggaatga atttatcaac aatcgatctt tttgagatca atgaagcatt
     2281 tgcagcttct tctatcgtag ttagccaaga actgcagtta gatgaagaaa aggtcaatat
     2341 atatggagga gcaatcgcat taggccatcc aattggtgca agcggtgcac gtatcttgac
     2401 tacattagct tacggattgc ttcgtgaaca gaagagatac ggtattgctt cattatgtat
     2461 tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat atggagcaaa cacataagga
     2521 tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa cggcgcagtc aattaataga
     2581 aaaaaatgta ttgacacaag aaactgcgtt gatttttcag gaacaaacac tttctgaaga
     2641 gctctccgat cacatgatcg aaaaccaagt gagtgaagtg gaaatcccaa tggggatcgc
     2701 gcagaatttt caaatcaatg gcaagaaaaa atggattccg atggcaacag aggaaccatc
     2761 tgtcattgca gctgcaagta atggtgcaaa aatctgtgga aatatctgcg cagaaacacc
     2821 tcagcggctg atgcgagggc aaatcgttct atcaggaaaa tcggagtatc aagcagtgat
     2881 caatgcagtt aatcatcgaa aagaagagct gattctttgt gcaaatgaaa gttatccttc
     2941 aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact cgggaattca tggggagttt
     3001 ccatgcatac ttatctattg attttttagt ggatgtaaaa gatgcaatgg gcgctaacat
     3061 gatcaactcg attcttgaga gtgtggcaaa caagttacgg gagtggttcc ctgaagaaga
     3121 gattctattc agtattttaa gtaattttgc tacagaatca ttggcttctg cctgttgtga
     3181 aatcccattc gaacgcttag ggagaaacaa agaaatcggt gaacaaattg ccaaaaaaat
     3241 ccaacaggca ggggaatatg cgaagcttga cccttaccgt gcagcaacgc acaacaaagg
     3301 gatcatgaat ggtatagaag ctgtagtggc agctacagga aatgatacac gtgcggttag
     3361 cgcttcgatc cacgcctacg cggcaagaaa tggattatac caaggattaa cagactggca
     3421 gatcaaaggg gataaactag ttggaaaact aacggtgcct ttggctgttg ccacagtagg
     3481 cggagcttcc aatatcctgc caaaagcaaa agcatcttta gctatgttag atattgattc
     3541 agccaaggaa ctagcacaag taattgctgc agtagggtta gctcaaaacc tggctgcttt
     3601 gcgtgcatta gttacagaag gaatccaaaa aggccacatg ggacttcaag ctcgttcatt
     3661 agcgatctca attggggcta ttggtgagga aatcgagcaa gttgcgaaaa aattacgtga
     3721 agcagaaaag atgaaccagc agacagctat acaaatattg gaaaaaatcc gggaaaaata
     3781 aaaaagcaat atctttagaa gaataaagca agatttacag ttattttatt tactgcagat
     3841 cttgcttttt
//
LOCUS       DAZUJO010000156         3836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000156 DAZUJO010000000
VERSION     DAZUJO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.164"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG8OL_002893"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG8OL_002893"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS4818789.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACG8OL_002894"
     CDS             complement(2261..2512)
                     /locus_tag="ACG8OL_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS4818790.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACG8OL_002895"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACG8OL_002895"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS4818791.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACG8OL_002896"
     CDS             3462..3680
                     /locus_tag="ACG8OL_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818792.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1063 a          815 c          712 g         1246 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttc
//
LOCUS       DAZUJO010000157         3655 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000157 DAZUJO010000000
VERSION     DAZUJO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.165"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..976
                     /locus_tag="ACG8OL_002897"
     CDS             116..976
                     /locus_tag="ACG8OL_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFS4818793.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="ACG8OL_002898"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="ACG8OL_002898"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFS4818794.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /gene="panC"
                     /locus_tag="ACG8OL_002899"
     CDS             1830..2678
                     /gene="panC"
                     /locus_tag="ACG8OL_002899"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFS4818795.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /gene="panD"
                     /locus_tag="ACG8OL_002900"
     CDS             2688..3083
                     /gene="panD"
                     /locus_tag="ACG8OL_002900"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFS4818796.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3285..3655
                     /locus_tag="ACG8OL_002901"
                     /pseudo
     CDS             3285..3655
                     /locus_tag="ACG8OL_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1425 a          412 c          722 g         1096 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tatcaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttatg tgaatcggct acactagact agacagaaaa
     3241 aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac
     3301 gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcaa tctctatctc gctggtaagc
     3361 ctcgcgcaga aattattcga gagtatgagc ttacgccttc ttctttcgat aaatggatga
     3421 agcaagcaca atcaacgggc tcattcaaaa aaagagacaa cttaacacca gaacaagcag
     3481 aattgatcgc actaagaaag aaaaataagc aactcgaaat ggagaatgat attttaaagc
     3541 aagcggcgct gatattcgga cgaaaagaca agtaattgat gccaacaagc ataaatattc
     3601 catatcagcg atgtgcaaaa ttctaaatat ttctcgtcag acctactatt atcaa
//
LOCUS       DAZUJO010000158         3647 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000158 DAZUJO010000000
VERSION     DAZUJO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.166"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2199)
                     /locus_tag="ACG8OL_002902"
     CDS             complement(<1..2199)
                     /locus_tag="ACG8OL_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFS4818797.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESLSESFNEARSSEPSITQESQIER
                     QEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVRVPRASIGLIEF
                     IRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITFEVAFFQSGGTE
                     PNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGNLGNLLIDGQDV
                     TNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREPISGGVRIAEVT
                     NPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYALEVTDVDLFNE
                     GRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNVINLNENELTYT
                     LHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRFSANNTIGGPTS
                     FTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKVIKTDENGQRLA
                     GAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYLLDATVHDVFIN
                     EYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGETLQTG"
     gene            complement(2871..3139)
                     /locus_tag="ACG8OL_002903"
                     /pseudo
     CDS             complement(2871..3139)
                     /locus_tag="ACG8OL_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1057 a          790 c          561 g         1239 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tacattaaaa gactcacttg tgctttcttc tggtacactg atttctattg agaccgtatt
     1861 tgattgaata tcaccgcttc ttgcggtcaa ttcgtattta cctgaatgct gggaagaata
     1921 agtaatcaga cgttcgtaat cattgagttg cttcacttcc ccttcaatca aatcctcact
     1981 cttatcaatg accaatgcat ttagcaggtc tggtgttcct ctgactcgca actggaactg
     2041 ctcacccaaa gcagcctcct cttgatcaac ttcgatcgtc agcgaacgct ctggctgttc
     2101 tgctaaagac tcagctagaa ccgttatgtt cccaaattga ttgatcacaa taagcataac
     2161 tagaaacata cttaacagtt tatgaaattt gttcctcact attactcccc cttataattt
     2221 tgactaaaca acaaggtacc cgataatttt ctaaaaagat tccccaactc aaaatggctg
     2281 cgttacctcc ctcgtatttt cggttcaact caagcgttgc aatgtaagct gtaaacaaag
     2341 ctaatgattg ccgattgtct tctgttgatt gattcgatag ctttgtatga cttttagttg
     2401 tccgtgtggc ttaaccataa ttatgatttc acgattcctc ctgccagtgc cttgcacaag
     2461 ctggagcgta accctaatag ttattttgtt aatgtactta tatactttta tacttttcca
     2521 ttagaaacaa tcactaaatc gttgatacca aggtcagact ctagaatttc atcagtgagt
     2581 ccctgtattg gttaaccttc atcaatccca aaaaaatata tcaatgctta gcatcaaaat
     2641 gaatatatag tttagaatac ttgggattca tcgataactg atcgcttatt tggccatatt
     2701 atttggcact tggttcaact aaacgcattt aggatgttcc ttcattctcg tcatggatgt
     2761 tctcaaaccg tcattactaa taaactgtcc accgaatcga tggttcattt cttgcatttt
     2821 ccacgtccaa ttactctttt tgctctttat ttgggcgtag tttcactttc ttaacttgta
     2881 tcgtcttcca tcaactcctt aatcatcttc ttagctttat tggctcggtt tccttgtaaa
     2941 aggtatctac aaatagtgac aatctgtatt aaatcttcat cattttctgt aatgtcaata
     3001 atttctatat tctgtaccat atattgcaaa gggtttcact aagttcataa ccgaagcgaa
     3061 ttaaacaact tttgtataga ataactacct tttctatttt tccattggta attctatcta
     3121 ttaattggtt taatcctttt gttgtagttg attccactac caagatgatt ctctcgaatt
     3181 ttaaagcttg aagatgtatt gctatttttg ctaactggcg tatttgcaac tatttttccc
     3241 ttctattctt ccttcattga ttcccctcta aaattactat ccataaaaaa ttgcataaat
     3301 gacaatagta tttatattag tcatactgct ttttaacaca gcaaattcca gaaaaacatt
     3361 cgttacatat ttgtgaattt ttccaaaaaa taacattttt ataaaacgac gaaaaataaa
     3421 tcactaccag tcaacttcta taatattacc aaataacaaa ctaactatcc atcaataacg
     3481 tgttttattt tttaaaattt ctataaaaaa acaatcattc ttaaaaagaa tatataaaaa
     3541 taacaataac ctttgtgatt tttcttgttt gaagaatgtt agaagaaaaa tctctaaata
     3601 cttataatta ttttataagc aaaaaaatgc tatcggtagt atcaccg
//
LOCUS       DAZUJO010000159         3639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000159 DAZUJO010000000
VERSION     DAZUJO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.167"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1484
                     /locus_tag="ACG8OL_002904"
     CDS             153..1484
                     /locus_tag="ACG8OL_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS4818798.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1450..1800
                     /locus_tag="ACG8OL_002905"
     CDS             1450..1800
                     /locus_tag="ACG8OL_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818799.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1892..2311)
                     /locus_tag="ACG8OL_002906"
     CDS             complement(1892..2311)
                     /locus_tag="ACG8OL_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS4818800.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2312..2656)
                     /locus_tag="ACG8OL_002907"
     CDS             complement(2312..2656)
                     /locus_tag="ACG8OL_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS4818801.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            3006..3602
                     /locus_tag="ACG8OL_002908"
     CDS             3006..3602
                     /locus_tag="ACG8OL_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS4818802.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          959 a          657 c          655 g         1368 t
ORIGIN      
        1 ttttcagtct tctttaactt ttttttgcaa aattaaaaaa atttggttaa attcaatggg
       61 ttcccttaca tttttagttt tgacctaaat gatagttgtc caaatctttc atttcaagtt
      121 atagttaaag aaaatgaaaa agaaggtgtg taatggaaaa cgaaactgac gttatttata
      181 ttcatcctca aaaacgaatc gtttcacaga aaagaaagta tttttatctt ggttttacag
      241 gtgttttttt tctttttatt ggcttgcttt ctaatactcc tactgataat tggagtggat
      301 tgctaactat tcttacctcg ccaagcaatc ttttgacaga ttattttgct ttaggtgttt
      361 tcggaagcgc attcataaat gtagggattt tgaccttact ttctgtgctt ttagcttatc
      421 ggcacaaggt gattttaaac ggtcccttat ttgcttcgat tctaacagtt accggtttct
      481 ctttttttgg caaaaacctt tataattcta tctcaatcat tttaggtgtg tatctatacg
      541 ctgtatttgt caataaacct ttttctcagt acatcatgat cggactgttt ggtagcgtat
      601 tatcaccggt cgttagttat atcacttttg gtatgcgttt tcctttactg ataggtattt
      661 tattggggaa tttagctggc atagcaattg gtatacttct tccgccctta gctgctcaaa
      721 ctttagtttt ccatcgtgga ttcacacttt ataatattgg attcacctct gggttgattg
      781 cgatgacttt tactgctgtt ttgaggttat tcagctattc aatcgtcgaa aatacattgg
      841 ttttcaatga atatcacttt cctctcattt ggattatttt tggctttttt tcgctgacag
      901 tcggaattgg tttctattat aattcctttc ggctttcggg gattcgagaa attttcgatt
      961 catctggaaa attgacaact gatttcattg ctaattctgg tattggtgct actttgatca
     1021 acatgggctt agttggtctg atgctgtctt cttatgtttt gctggttggc ggtcagttaa
     1081 atggacctgt catcggtgct attttatctg cggtgggttt cagtgctttt ggttgtcatt
     1141 taaaaaacag ttttccgata ctagtcggaa tatttattgc ttctttgttt ggaacttttc
     1201 atgagatcac cagtactggc atgcttgttg cagctgtgtt tgggacaggt cttgcgccca
     1261 taagcggttt ttacggcagt ttttacggtg taatagctgg tgtgctacat attgctcttg
     1321 tccataacgt cagcactttg cacggaggac taaatctata taacagtggt ttttcaactg
     1381 gtttcgttgc aggtatcctt gtaccgatac tagataactt taccgctgtc agaaaggaaa
     1441 aaaagacact tggaaaaaga attatcaaaa agaatcatcg atgagcttct tacttattta
     1501 tattcccatg aaataactga aatacgtatt ggagtagaat ttgctgagga agggcttttc
     1561 atagagatac agggtacggc agaaactcag ccagaagatt tagatgtact tgctgatttg
     1621 ctgaacaatc ctcgggaccc tagtatggaa atctactatc aagaactatt aggtaatccc
     1681 cgacatgatc gggaagatta tcatctgtta ggtagtttga ttgatgacgt ggaaatcatg
     1741 tatgcccagc cgattatcaa tattaagatc tttcgacgtg cttctcattc ctattattaa
     1801 ccctattttc tatttataaa agactgaatc aaaaaagcag ctcctgaaag ttcgattaaa
     1861 atctactttc aggagctgtt caagcttgac attattgatt ttccaatcct tcatacactt
     1921 catctgttac aggttctaaa aactctgaat taccagcagt gatcgccaaa tgttcaaacc
     1981 aactgtcttt tgtcgcacca tgccaatgtt taatcccttt gtgcgttacg acgacatctc
     2041 cagccattaa tcgtcttgct ggctttcctt cttcctgata ccagccttct cctccggtca
     2101 caagtaagat ttgatagccg tctgtatgga catgccagtg attgcgacaa cctggttcaa
     2161 aagttacatt acctacacca acagaaactt cgggatcatt cactaacata ttcagatagc
     2221 tttgcccggt aaatacttga gcgtaagctt cgtttttttc acctgctgga aaaatcacgc
     2281 cattgtgtat ttcttcgtgt tttgtcatca tttacgcctc tttcttatct tcgctatttt
     2341 cttctaaata gatttctttt gctaaactaa aagctgacca agcttttggc cattctacat
     2401 aaaaagctaa atgggtaatg atttctgcta tttcttcact agtaatgcca ttttgcttgc
     2461 caatcttcag atgagctggc aattgttcaa aatttcctcc tgaaattaat gcagatatgg
     2521 tgatgatact acgctgatga gcagataatt cttctctgga ccatacttgt ccaaataaaa
     2581 catcatcatt taattctgca aattttggtg caaaatctcc taattgatct cgtcctgcgg
     2641 tttgtttttc tgccatttct cttattctcc cttcacgact gttaggtgat tcaactatgg
     2701 ttctaaggca actttatttt ctattgtttc ttcgcatttc attttaaatg tagtctttca
     2761 gcctgtccaa tgcttagttt attcagtcga tatgcttttt aagcatagtt gctaacgctt
     2821 tttttatttt aaaaaaaaca agttattaaa taagtcttta gttggatgtt tttttgagat
     2881 caatctttta tacttatgat gaacttacaa aattgtttgt tttctgtcat atcaaaaaat
     2941 ggcgcagtta gttgcttttc tagtaaagta tagttaacaa agaacctcta aggagttgat
     3001 gaataatgaa tattgctgcc tctcgaaaac tgataaattt catatccatt atcggattag
     3061 gcctatcaat cgcgctgacg atttatttta ttaatttagg tgttttcaaa gatttgaatg
     3121 cgttaagagg attagtaggt gattccatca ttttaggacc gattattttt gtcttcatcc
     3181 aaatcctgca agtagttatt ccaatcattc ctggagggat cagtactgca gctggtgtcc
     3241 tgatatttgg cccttatgct ggatttatct ataactatat tggaatctgc attggttcga
     3301 ttatcatttt tctgctaggg cgtcgttatg gaaagccatt catactttcc atgattagcg
     3361 ataagacata taacaaatat attggctggc tggataatca aaaccgtttt gagaagctgt
     3421 ttgctctagc tatttttctt ccagttgctc ctgacgatgc gttatgtttg atggctggat
     3481 tgacaaacat gtcagtgaaa cgatatacac taatcatttt gattgctaaa ccactgtcga
     3541 ttttcttata tagtatggcc ttgatttatg gaggtcaata tctaagtggt cttcttggat
     3601 aatatttatt ttgtaaaatt agtcattgat tgacaatgt
//
LOCUS       DAZUJO010000160         3440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000160 DAZUJO010000000
VERSION     DAZUJO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.168"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..920
                     /locus_tag="ACG8OL_002909"
     CDS             120..920
                     /locus_tag="ACG8OL_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS4818803.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            936..1814
                     /locus_tag="ACG8OL_002910"
     CDS             936..1814
                     /locus_tag="ACG8OL_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFS4818804.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1827..2807
                     /locus_tag="ACG8OL_002911"
     CDS             1827..2807
                     /locus_tag="ACG8OL_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS4818805.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3078..3170
                     /locus_tag="ACG8OL_002912"
     CDS             3078..3170
                     /locus_tag="ACG8OL_002912"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818806.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT         1184 a          561 c          719 g          976 t
ORIGIN      
        1 aagattgttt ttaagctaat tattatatgt gtgttattga actaactctt gacagataat
       61 caaaaagaat ttatagttgt taccgtagta gctactaaaa ataaagtgag gattaatcaa
      121 tggaaacgat tattcaaatc atgaaaaaat atgatttttc agagatggag acaagagtgt
      181 ataccacact attagaaaaa ggtaatctta caggatatga agtaagtaaa atctcaggtg
      241 tgccacgctc gaaagtatat aatattttgg aaaaactatt aaaaaagaat ttgattgtcg
      301 ttaataaatc agaacctaaa ctttatcatg ccatctcggc aaatgagttt ttagagaaat
      361 tagaaaaaag tgttaaaaat gatctttctt ttcttaccaa gaatctaggg atgatcaaag
      421 aaaaagatga agaagaaatg ctgtggaaag ttgatggtat cgagtatgtt ctggataaag
      481 tagagcattt ggtgaaaaac gcaaaagaaa gtttgctcat tcaagtttgg catgagaatt
      541 tcacggattc actactaaag gcattgcagc aagctgaaaa aagagttgat aagtttgtac
      601 tgattttatt cagttctacc catgaatatg atcttccctt agaaaagtat tatattcatg
      661 gttttgaaac agacaaactt gctgactttg gagcaagatg gatcaacatc gtcgccgatg
      721 agcaagaagt cgtgtttggt accattaacg aagaactgca atcaaccgat gtcacttgga
      781 caaaaaacca cgccatggtc aatctagcaa aagaatatgt caaacatgat gcatatacac
      841 taaaagtgat tgcagaatct cctgaagaac ttaaagcaaa atatggtgag ggctttgagg
      901 gaatcagaaa gatttactag taggaggaat tttttatgat tcggaatatt ttgctgacac
      961 taattgtcgc aatcaatcta tattttatta tttactttct aagagacctt ttcaaaaaca
     1021 aacaaaattt caaagaagaa ccaggggata tgcgtctgtt gcccttcacg tcatttatca
     1081 tcttctttct ttcaacattt ggcgtttccg actttgctat cggaaccgtc ctttatccaa
     1141 agttgaaatg ggtcagcatg aaaaaactac ccggaacgtt gaatacccaa tgtgtagtac
     1201 cggtagccgt catggcattg tcttacatca ctgccatcaa cgtagggatc aagacactag
     1261 ctgtttgtat tatctgccaa attatcggtg catatttagg accaagattt gtcgtgaagt
     1321 taccagagaa aacgatcaaa ctgtttgtcg gcataggatt ggtcattgct gcattgttga
     1381 tttttgcagg acaaatgaac tggattccat caaacggaac agccagtgaa ttgtatggcg
     1441 gaaaattaat cttggcagga tttttactct ttgtctatgg tgcattgaac aatatcggca
     1501 ttggttctta tgcgttaacg atggtaactg tctacttgct aggcttgaat ccagttgcag
     1561 cctttcctat tatgatgggg gcttgtacct tctcagtacc gatcggcagt gttcaattca
     1621 ttaaatttaa tgaatacagc agaaaaatca cactttttac ttcaaccttc ggtgtattgg
     1681 gggtattggt cgctgtattc ctagtcaaat ctttagatac ctatatcctg aaatggatcg
     1741 tgattttggt cttactatac agtgcatata cgatgttggg cagtcaatta aaaaaagtta
     1801 cagcagctaa ttaaggagcg attcaaatgt tatttcttaa aaaagaagat attatcaaaa
     1861 gtttttcaat gagagaagcc atcgatgcgg acaaaaaagc actgtcattg tattcagcag
     1921 gaaaggcaag tgtgcctctt cgcaccaata tcgatgtgcc aaaaagcaac ggacaaagct
     1981 tatatatgcc tgcttatgta gaaggtggcg aaggcgcgct aggtgtcaaa atcgtttccg
     2041 tgtatcctga aaacatcaag aaaaacttac caagtgtacc agcaacgatg atcgtattgg
     2101 atccagaaac gggtatggta tccgcttgtt tggatggtac ttatttgaca caattaagaa
     2161 caggcgctgt tcaaggagca gcaacagaac ttcttgcaag agaagatgct aaaattggcg
     2221 cgctgattgg aaccggcgga caagcacagt cacaattaga agcaatgttg accgttcgta
     2281 aattagaaga agttcggatt tttgatattg attttgagcg tgccagccaa tttgctgaaa
     2341 aaatgatgca acaattttct gtaacgatgc gcccaacaaa aaccagtcaa gaatgcgttg
     2401 aaggagcaga catcattaca agtgtgacca cttcaaaacg tgcgacattc tctgctgagt
     2461 gggtcaaaaa aggtgcgcac atcaatggag tcggtgctta cacaccggaa atgtgtgaaa
     2521 ttccacgaga aataatcaaa gccgcagatg ttgttatttt tgataccatg gacggtgttc
     2581 taaaagaagc tggggacttc atttcaccgc tgcaagatgg gtatattcaa agagacagct
     2641 atcatgggga actaggccaa ctgatcaatg aagaattgat cggtcggaca agtggagaac
     2701 aaatcacgat cttcaaaaca gttggttcgg ctgtattaga tgtggtagtt gcgacagaaa
     2761 tcgttaagaa agctaaagaa aataatttgg gaaaattgat tttctaatta aaaaggctga
     2821 aattaaaacc tttattattg cgtttaaaaa aagaaaacaa agaagtttgt aatcaaatgg
     2881 aaactgaccc cataatttaa ataaaataaa aaacatctct tgttgaattc tagtaatgaa
     2941 gcgaccctaa aacttagacc aaaaaatcta acttttgggt ggagttgtca agaaaaaccg
     3001 ggcacttagt taaaaaataa gttggacggg ttatactaaa attgacacca aattaagaga
     3061 aagcgaagat ctggattatg ccaagacgtt catttgatga aatatttaaa attaccgctg
     3121 taaaactggt aacggaagaa gggttttctg ttaaagaagt tagtctatag ctagaagctc
     3181 gctctaatag cgtgtattga tgagttcaag aagttgaaaa atatggataa cgtacttttc
     3241 tcggtagagg aagtcatgaa atccattcgc agaaatgaaa ttacacgctt agaaaaagag
     3301 aataaacggt taaaagaata ggtagaactc ctaaaaatta ccgaagtctt cctgaaagaa
     3361 aaggctcttc gtcaaattgt gttggcgaag acaaatttga ataaaactta gtccgatatg
     3421 cggcgcgttc tgtgcgccgc
//
LOCUS       DAZUJO010000161         3355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000161 DAZUJO010000000
VERSION     DAZUJO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.169"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1014)
                     /locus_tag="ACG8OL_002913"
     CDS             complement(<1..1014)
                     /locus_tag="ACG8OL_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS4818807.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRT"
     gene            1172..1501
                     /locus_tag="ACG8OL_002914"
                     /pseudo
     CDS             1172..1501
                     /locus_tag="ACG8OL_002914"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1549..2949
                     /locus_tag="ACG8OL_002915"
     CDS             1549..2949
                     /locus_tag="ACG8OL_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS4818808.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            2982..3149
                     /locus_tag="ACG8OL_002916"
     CDS             2982..3149
                     /locus_tag="ACG8OL_002916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818809.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            3203..>3355
                     /locus_tag="ACG8OL_002917"
     CDS             3203..>3355
                     /locus_tag="ACG8OL_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS4818810.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          961 a          612 c          670 g         1112 t
ORIGIN      
        1 tgtacgaatc cttcggcgga tattttcagg aaaagacaaa atttgaatag catcttcaaa
       61 gccgttttct aaaatgtcgc aagcagcact taattttgaa tcacaaccat atttttccaa
      121 gaagtgctct tttctttcac gtgtcaattc aagttcagag gctttaaaga tactcttcaa
      181 ctcgtctttt acatcagaag aaactttctt tggcagctta tcaaaaatat ttctcaaaaa
      241 gtgggcttgg catctttgcc agctcgcacc taaaaagttc ttgcgaacag ccttaactaa
      301 ccctttgtgt gcatcagaaa tgaccagttt tacaccgaac aaacctcgtt gaacgaacga
      361 ttgaaaaacg gtatcccaat tatcttccga ttcgccgtct tgaacatcaa aaccaagaat
      421 cgtacgtcgt ccatcagaat caatgccgat accaatatat acaccttttg agacaatacg
      481 atgattttcc cttactttca tatacagggc atcacacatg aaaaatggat attccgagcc
      541 ttctaggctc cggtttctcc actcaaaaac aaatggatcc aactgtttca tcagatttga
      601 aacgaatgat ttagaaacag tcgcaccatc cgttagcagt tcaactgttt tagttacttt
      661 gcgagtggag acaccagaaa tgaccatttc aatcatggca gcgatcaaag ctttttcgct
      721 tcgctgatat ctttcaaaga tagtaggact aaacttaccg tctctggttc gtggaacatg
      781 aagttctaac gtaccaattc gagttgtaaa cgaacgatcg tagtaaccat ttctttgtga
      841 ttgccgttct tctgaacgtt cataggcatt tgcctgaata tattgatttc gttgttcttc
      901 catcagctga ttaaacatga tagtcataag tgtttttgcg gtatcattcg caccgctttc
      961 gtttataatt gcttgaattt cttcgctttc aaaagtaaaa tgtacttgag tcattgatat
     1021 cagtcctatt cgagtatgtt ttctgcaaaa aacattgtac tcctaactga gatcaatggc
     1081 ttttttgatg cttcactttt acacaattat atggacgtaa tcaaaaaatg tttccacaga
     1141 ggacttcttt aattattgtc gattagagga aagtggtgta acagtcacta gcggtacaga
     1201 tttaccgctg tttattactg atattcctga atcgattata agagcagtat tccgtcgttt
     1261 tcctgaagga gaagaagtct gggagaaaga aacaagcttc acgacaaccg agctgctgaa
     1321 aagttttaca agcaacagct tcaaagtaaa tggtttatcg gagtacggag aaataaaagt
     1381 agggaaaagc gccagcttag ctgttttcag cgaggacctc tgtactgatg actggcaaaa
     1441 agtgatggct gcggaagtta tcgctaccta tcttgatggt gacttagttt acaagaaata
     1501 atgcttttcg atataacgtt ataaaacgaa ttaaagagag gttaaaaaat ggaaacaatg
     1561 aaaagaaaat tcaccttccc acatagttat acattgattt ttatcttaat tattttggct
     1621 gcaattttaa cttggataat tcctagtggt cagtttgaga cagttgttga aaacgtcgca
     1681 ggtatggaaa aaacaacggt tgttccggga acctatcacc aaatcgataa ggcagattat
     1741 ggacaaggtt tttcctctat actaatggca ccagctgaag ggattatttc agctgttgag
     1801 gttgttgctt ttgttctcat cgttggtggt gccttcggga ttatcctaaa aacgggagcc
     1861 atcgatcgag ggctgtttac cttagcaaaa gttctgggag acaaggggat cttagttatt
     1921 ccgatgtcga tgattctatt cagcttagga ggatcaacct ttggcatgag cgaggaagtc
     1981 attcccttat ttgcggtgtt tatttctctg atgttttctc ttggctatga ttccatgact
     2041 gcgatcttga ttctattttt aagtacacaa gcgggatatg ttgggtcgac gattaatccc
     2101 ttcaacgttt taatcgcaca gggagtagct ggcgtttacg gaaatcccct gctttggttt
     2161 cggatgctat cctggattgt cattactggg atttcaattg ttttcaccat gctttatgct
     2221 agcagagtca aaaagaatcc actgtcctct attgtttatg agagtgattt aaaggttaag
     2281 cataagtttc aaaattttga cactgaaaaa gagttttcaa cacaagataa actaattatc
     2341 gccggtttta tcgtaggttt aggaatcatg atttggggta tcatttccaa gggatggtat
     2401 atggttgaaa tttctgcact attcttagga ttaggtcttt ttgctggggg catcgctaga
     2461 ttttctcaaa aggaaattgc tgaagctttt gtcgaaggct gttcagattt cgcctatgct
     2521 gctgtaatca ttgggctagc tagaggagtc ttggtaatct tggaaggtgg aatgattatt
     2581 gacactattc taaacagctt ggctaacctt ttaggtggtc ttccaaaatt cctgttcact
     2641 acgattctac tcatttctca agtaattgtt tccttttttg ttccatcctc ctcaggagct
     2701 gcggcattaa cgatgccggt tatggcacca ttgggtgatc taatgaatgt aagtcgtgat
     2761 gttgttgttc taagtaacca gtttggaaat ggtttaatga atattatctc cccaactagt
     2821 ggtgtattac tggcaggatt agccatcgca gagattaatt tctctaaatg gtttaaggtt
     2881 ggcttcaaaa tatttgccat cttaaccctt gtttctgctg ttttgttata cattgctaca
     2941 ctcttttagt cttaatcatc cattaatagg aggcaatagt tatgattaaa ataaatcaat
     3001 tagcaggaat gaatatccac tatttatttt attctctcaa agaatttttc gcttatcaac
     3061 agaaaattgg tatttcttct gttgaacttt ggggaggtgc tccgcatttt ttcatagatg
     3121 cttttactgg ttctgttgca aagttttaaa tctactatca aataaggtag aataatagaa
     3181 aaagatagca ggaggaatga cgatgaatca ttttaaagga aagcaatttc agcaggatgt
     3241 gattattgta gccgtgggct actatcttcg ttataacctt agctatcgtg aagttcgaga
     3301 aatcttatat gatcgtggca ttaacgtttc tcatacgacg atttatcgtt gggtg
//
LOCUS       DAZUJO010000162         3243 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000162 DAZUJO010000000
VERSION     DAZUJO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.170"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..2165)
                     /locus_tag="ACG8OL_002918"
     CDS             complement(552..2165)
                     /locus_tag="ACG8OL_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS4818811.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="ACG8OL_002919"
     CDS             complement(2306..3214)
                     /locus_tag="ACG8OL_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4818812.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1045 a          557 c          572 g         1069 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctt
//
LOCUS       DAZUJO010000163         3221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000163 DAZUJO010000000
VERSION     DAZUJO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.171"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8OL_002920"
     CDS             301..585
                     /locus_tag="ACG8OL_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818813.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8OL_002921"
     CDS             588..1004
                     /locus_tag="ACG8OL_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818814.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8OL_002922"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8OL_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS4818815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG8OL_002923"
     CDS             2544..2630
                     /locus_tag="ACG8OL_002923"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS4818816.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG8OL_002924"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG8OL_002924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818817.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZUJO010000164         2955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000164 DAZUJO010000000
VERSION     DAZUJO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.172"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..406
                     /locus_tag="ACG8OL_002925"
     CDS             89..406
                     /locus_tag="ACG8OL_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS4818818.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="ACG8OL_002926"
     CDS             complement(497..862)
                     /locus_tag="ACG8OL_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818819.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="ACG8OL_002927"
     CDS             1070..1552
                     /locus_tag="ACG8OL_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818820.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="ACG8OL_002928"
     CDS             1749..2639
                     /locus_tag="ACG8OL_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS4818821.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1010 a          460 c          566 g          919 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tgtaa
//
LOCUS       DAZUJO010000165         2948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000165 DAZUJO010000000
VERSION     DAZUJO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.173"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..456
                     /locus_tag="ACG8OL_002929"
     CDS             175..456
                     /locus_tag="ACG8OL_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818822.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            458..874
                     /locus_tag="ACG8OL_002930"
     CDS             458..874
                     /locus_tag="ACG8OL_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818823.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(1211..1351)
                     /locus_tag="ACG8OL_002931"
     CDS             complement(1211..1351)
                     /locus_tag="ACG8OL_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFS4818824.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAV
                     VN"
     gene            complement(1348..2322)
                     /locus_tag="ACG8OL_002932"
     CDS             complement(1348..2322)
                     /locus_tag="ACG8OL_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4818825.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          994 a          603 c          438 g          913 t
ORIGIN      
        1 aaaaaaaagt gggtagatag gctgtgccta tcacgtttct cagaaaaaaa tttgatatca
       61 ctttttcgcc atcgtggcta gcttatgtat agtattttag caaaaaacag ctatacaaat
      121 actatattcg tatgatatac ataaattata cacaaatgcg aaaggagttt ttctatggac
      181 attttagttc ggaagatcga ttcaaaggtt attgcaaaaa tagacaaacg ttgccaagaa
      241 ctcacaaata aaactggcaa aaaatggagt cgaaatcgct acttaaaagt cctaatcgaa
      301 aacgattttg accacgcact tatgcattac aagaaagatc aattcgattt attactcgaa
      361 aaatttatcg ctattcaaga aaaaaatact gaaacgctga atgaatatgt tcgtacaaac
      421 aaccaactca ttgaaacact tatcggagga agataacatg agaaaaactt ctgttcaaaa
      481 acacgttacc ctttatccca gtcacgtgga cgttttagat tccattatcg aaagaaatcc
      541 agatatcaac aattactcgg aagccattcg ttttttatgt atgagttatg aacctgatac
      601 caacacgact actaccagca atcaagccaa actaaatgct atgggaaaag aaatttccat
      661 catgtccgaa atcattgcag aatttgctga tttatatcta aaagatacag gtattcttat
      721 tggcacaaat tctaaagtgt atcaagaaac gaaagaaaaa attgaaaaac aacttaatcc
      781 ctccaaacga aatcaaccaa aaaatcgtca aaaagaaacc aatcaaacat ttacttctaa
      841 aaatttcgac ccaacaaatt ttctaaaaag ataaaaaata aaccaggaga tttaaattcc
      901 tggtttattt tttgggtagg atcaccatct taatagcaac atttacaata agatagtaac
      961 caattgaatg aaaaaaacga atttcagaag aaataacaga aaaaaagata aaaacaaaaa
     1021 aattataaat acatgatttg aattcataat cgtaaaaaaa gctgctcgta cgagcagcta
     1081 aataagaaaa agattggtat gttttaagga taaaataagt ataacaaatt tttcacatag
     1141 agcaaagggg gcgaaaatcg cccccttttt ttcgttttaa aaccccaaaa cccaaagaca
     1201 taaatttcgg tcaattgacc actgcatttt tttctttata ctgagcaagg tcaaatcccg
     1261 aattgaccag ttgattgcgc accgtatttt tcgatacacc caattcatca gcaagttttt
     1321 ttagggtcat tttttctatc gaatcactca ttcagcacct acttccgtca catcaagtcc
     1381 ttgaagccat gtatctaatt ctcgtttcaa atcatctgcc gactccgaaa atgtttctcg
     1441 caaatgattc gtcaactcat cattttgact ttcttgcttt aacaattcac gatatttata
     1501 ttttgaattt ctatgaattt gcccccagta actatgttcc gttataagag cagtaatcat
     1561 cattctgtca gttactttca catcttctgg caaatagtga ggacttgcaa agctatctaa
     1621 actttcatga accattgcat gccaatcagt tgctttcccc cttcgcaatt gcaattctag
     1681 tcgccaccaa gtatcgattt ctttcggaac aactttccgt tttctttctt gttccaattt
     1741 tttgttatac atccgaatct gtcgttcact agcacgactt ccccaatatg ctgtttccag
     1801 tttcccagac cgaccgtaaa ttggtttgaa agaaacagga tcaaccaccc gatattgggt
     1861 aatgaaatca tctggcacgt caataatgtc acaagcaata tctgcacgtg agaaatgtgg
     1921 ttctaaaaat aaatcatgga taaagttctt catcgagccc gccagaaatt ggctaatttt
     1981 attggggtta aagtcaatcc gcccttttcc ctcttgaaac ttaagaatct caatataagc
     2041 tatattttcc tcatgttggt ctagtaattg ccagccatta tttccaaccg cttttaaata
     2101 tccgttacct tcgtttagac gcataagctg ttcaaaatct aaaatcaaca catcatgggg
     2161 agtgtgataa taaatatttt cccttaatgt accaacaatg gtaattcggt caattgacca
     2221 ttgcattttt aagggggtgc acatagtttg tgcatttaac cgcctgttag atatgcggtt
     2281 aagtttttcc cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa
     2341 gaaaaaagag atttttggaa agaatttcca gagttcacgt aactctaaaa atccatatcc
     2401 aaaaatctct agcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac
     2461 taggtgagta gtttccgtaa ttagccgaag ggtgcttcca acaccgttcg gcttttattt
     2521 tgctcttttt tctcgttttc tttgattata gcactcctct taaaaaaata aaaagacctc
     2581 agggaatttc attccccgaa ccccttgagc ctcctcctgc cgaaattatt tcacgcttcc
     2641 gctcttcatt tcattcagtt ctcggcttgc acaatctaac acgccgtttc gctaccagtc
     2701 gccttacagg ctccattctc gcttctctta cgtgttttaa cgattgtgcc gataactaaa
     2761 cgaaatgaac gctaaaacgt cttagaacgc aaaatacgcg cttataaaat catttcatct
     2821 tgattacatg cccgtcataa tcgttcagtt tagtttcatt ttcgtaaact ttttttattt
     2881 ttacgaacag aaaaaagtcg cacatcacca ttttataaag agcataagga aacgtgtacc
     2941 cactttct
//
LOCUS       DAZUJO010000166         2860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000166 DAZUJO010000000
VERSION     DAZUJO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.174"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..2145
                     /locus_tag="ACG8OL_002933"
     CDS             151..2145
                     /locus_tag="ACG8OL_002933"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS4818826.1"
                     /translation="MVLKQGKQDYQIIGYLEDDLNKIKINSDDVMYFFMTPLSDFPSS
                     VIDNVEGIQIYSNTLTKFGFIDDINESHSTRAQSLKEFLDPYIIVCTPKIKRNHEQAR
                     NYYQGDDLELIPKPKSEVFKNLIKENESLVPIPVFSINNSDSVIKDETSFENQLLNGK
                     LLGKIINFSKDNEDYPLNVFWENTDGSRVLFGEIIGQNNSMYGVAYRIEKDNFYKKFI
                     DEGSKNYVGYDDFINQTKDIIFVPYLLLQKIIETKKDINEGIENISDSKISPVSSIIT
                     EQTQIDISELEDKHAIQQNDHFSEHNEFIERFNQLTIRSKLHYSKRDLWNFHTAMLGD
                     SLVVLAGLSGTGKSQLVTSYAKALQLSDTQIKFISVRPFWEDDSDLLGYADTVNSVYR
                     PGDSGLIDALLEASRHKENIYMVCFDEMNLARVEHYFSQFLSVLEMEPNTRKIKLYNE
                     DLENKLYNSSTYPSTIEVGQNILFVGTINTDESTHQFSDKVLDRSNIISLELVPFHEI
                     SEVNDYIQSDQKKRNQLKYDDFKNFKNSNPTNGLTQKEKEMFWKLHLAINSKDKNIGI
                     GWRILKQIDEYLKNLPVQNDLSRKEAIDIQLVQRVLSKIRGSDEQLSELVGKWNENDL
                     KQVGEFEQIINEYPESSDFKYTKKILLQKARELNLHGFTL"
     gene            2129..>2860
                     /locus_tag="ACG8OL_002934"
     CDS             2129..>2860
                     /locus_tag="ACG8OL_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818827.1"
                     /translation="MDLPFSLKLNFGYKSEEQEYELLFFQDEASLLEYTPVKVKEFSN
                     LSFTFNSIDKHAKLYIPELDDYLIENQVKEENGDICYIPSDDNIIIYNRDQGKTVPLI
                     PGYYTLYVKIGETIFYSRFEIILKDLGTPEWEKMKIEIEKMVGGLARTFIPKKTSRRE
                     ITTSNYLMNDQLFQRILEFENNIPQAIVSLESLKKEAKYRIDKRYNWKPIGSKNLVDQ
                     KTISQLQIHPEKRGLLYTPSRIIKYD"
BASE COUNT         1150 a          376 c          442 g          892 t
ORIGIN      
        1 taattgaaga aaatgatatt aatgaattat ggattacaac ctttcaatta attcccagaa
       61 tgaaaaaaaa gattatacaa agtttaaaaa ataaaataaa aattcaagaa ttaaatgatt
      121 ttactatgct tatgaaggag gaaaatataa atggttttaa aacaaggtaa acaagattac
      181 caaattattg gttatttgga ggatgattta aataaaatta aaataaactc ggatgatgta
      241 atgtactttt ttatgactcc tctttcagat ttccccagta gtgtgattga taatgtagag
      301 ggtatacaaa tatatagtaa tacgcttact aaatttgggt ttattgatga tataaacgaa
      361 tctcatagta ctcgtgccca atcacttaaa gaatttttag atccttatat tatcgtttgt
      421 acaccaaaaa ttaaaagaaa tcatgaacaa gcaagaaatt actatcaagg tgatgattta
      481 gaattaatcc ccaaaccaaa gtctgaagtt tttaaaaatt taataaaaga gaatgaatca
      541 ttagtaccta taccggtctt ttctataaat aacagcgatt ctgtaatcaa agacgagaca
      601 agttttgaaa accaattact aaacggcaaa ctactcggaa aaattataaa tttctcaaaa
      661 gacaatgaag attacccttt aaatgttttt tgggagaaca ctgatggatc aagagtttta
      721 tttggtgaaa taatcggaca aaacaattca atgtacggtg tagcatatag gatagaaaaa
      781 gataattttt acaaaaagtt tattgatgaa ggtagtaaaa actatgtagg ttacgatgat
      841 tttataaatc aaacaaaaga tataattttt gtgccctatc tattacttca aaaaattata
      901 gaaactaaaa aagatattaa cgaaggcata gaaaatatct ctgattccaa aatatctcca
      961 gtaagttcaa ttattactga acaaacgcaa atagatatat ctgagttaga agataaacac
     1021 gctattcaac aaaatgatca cttctctgaa cataatgaat ttatagaaag atttaatcag
     1081 ctcacaattc gtagtaaatt acattactca aaacgagact tatggaattt tcatactgct
     1141 atgttgggtg atagtttggt ggtattagct ggactaagtg gtaccggtaa atcacaatta
     1201 gtaacatcct atgcaaaagc acttcaatta tcagatacac aaataaagtt tatttctgtg
     1261 agaccctttt gggaggatga ttctgattta ttgggctatg ctgatactgt aaattctgtc
     1321 tatcgtccag gagatagcgg tctaattgat gcactattag aagcatcaag acataaagaa
     1381 aatatttata tggtttgttt tgatgagatg aatttagctc gggttgaaca ttatttctcc
     1441 caatttcttt cagttttaga aatggagcca aatactagga aaatcaaact gtataacgaa
     1501 gatctagaaa ataaattata taactcttca acctatcctt ctacaattga agtaggacaa
     1561 aacatattat ttgtaggaac aataaataca gatgaatcta ctcatcaatt ctcagataaa
     1621 gttttggata gatcaaacat tatttcactt gagttagtac cattccatga aattagtgaa
     1681 gtaaatgact atattcaatc agatcaaaag aaacgtaatc aacttaaata tgatgatttc
     1741 aaaaatttta aaaactctaa ccctactaat ggtttgaccc agaaagaaaa agaaatgttt
     1801 tggaaactcc atttagcaat taattctaaa gacaaaaata tcggtatcgg ttggagaata
     1861 ttaaaacaaa ttgatgaata tttaaaaaat cttccagtgc aaaatgactt atcaagaaaa
     1921 gaagctattg atattcagct tgtacagaga gtattaagta aaatcagagg ttctgatgaa
     1981 caattatcag aattagttgg taaatggaat gagaatgatt taaaacaagt gggtgaattt
     2041 gaacaaataa ttaatgaata tcctgagtct tcagatttta aatatactaa aaaaattctt
     2101 ttgcaaaaag ctagggagtt gaatttacat ggatttaccc tttagtctga aattaaactt
     2161 tggttataaa agtgaagaac aagaatatga acttttattt tttcaagatg aggcatcgtt
     2221 attagagtat acaccggtta aggtaaaaga attttctaat ctaagcttta catttaacag
     2281 tatagacaaa cacgcaaaac tatatatacc agaattagat gattatttga ttgaaaatca
     2341 agtaaaagag gaaaatggag atatttgcta tattccaagt gatgataata taatcattta
     2401 taatagggat caaggtaaaa cagtacctct tattccaggt tattacactc tttacgttaa
     2461 aataggtgaa actatctttt attctcgatt tgaaataata cttaaagatt taggtacacc
     2521 agaatgggaa aaaatgaaaa tcgaaattga aaaaatggtt ggcggtcttg ctagaacttt
     2581 tattcctaaa aaaacttctc gtcgtgaaat tactacttca aattatctaa tgaacgatca
     2641 actttttcaa cgaatattag agtttgagaa caatatacct caagcaatag tttctcttga
     2701 aagtttaaaa aaagaagcaa aatatagaat tgacaaacga tataactgga aaccaattgg
     2761 aagcaaaaat ttagtggatc aaaaaactat ttcacagttg caaatccatc cagaaaaacg
     2821 tgggctacta tatacaccta gccgcataat aaaatatgat
//
LOCUS       DAZUJO010000167         2796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000167 DAZUJO010000000
VERSION     DAZUJO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.175"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8OL_002935"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8OL_002935"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4818828.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1085 a          435 c          569 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DAZUJO010000168         2657 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000168 DAZUJO010000000
VERSION     DAZUJO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.176"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..153
                     /locus_tag="ACG8OL_002936"
     CDS             <1..153
                     /locus_tag="ACG8OL_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818829.1"
                     /translation="SLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPKE
                     ILSKIS"
     gene            163..819
                     /locus_tag="ACG8OL_002937"
     CDS             163..819
                     /locus_tag="ACG8OL_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS4818830.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            819..2195
                     /locus_tag="ACG8OL_002938"
     CDS             819..2195
                     /locus_tag="ACG8OL_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818831.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            2325..>2657
                     /locus_tag="ACG8OL_002939"
     CDS             2325..>2657
                     /locus_tag="ACG8OL_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4818832.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1053 a          314 c          468 g          822 t
ORIGIN      
        1 agtttaataa ataatagtag ttttatgtca aatataacaa cacttggaca aagttattta
       61 atattaataa gtattattgt tttatcagtt gtatttgctt cttcattaat gttaatcaag
      121 aagcctaaag aaatcttatc aaaaataagt taggagaaaa taatggatat attagaaata
      181 aagaatgtag catatagtta tgcaaattct aaagaaaaag ttttgtcagg agtaaatcaa
      241 aaatttgaac ttgggaagtt ttatgcgata gtaggaaagt caggaacagg aaaatctaca
      301 cttctttcct tacttgctgg acttgataaa cctcaaacag gaaaaatatt gtttaagaat
      361 gaagatatac aaaataaagg atatagtaat catagaaaaa ataatatatc tttagtattt
      421 caaaactata atttaataga ttatttatca ccaattgaaa atattagatt agtaaataaa
      481 tcagcagatg aaagtatctt gtttgaacta ggtttagata aaaaacaaat aaaaagaaat
      541 gttatgaaat tatctggtgg acagcaacaa agagtagcta ttgctagagc attggtatca
      601 gatgctccaa taatactagc tgatgagcct actggtaatc tagacagtgt tactgctgga
      661 gaaataatta atatattaaa gacattagct aaagatagaa ataaatgtgt aatagttgta
      721 acacatagta aggaagtagc agattctgcg gatatcattt tagaactaag tggtaaaaag
      781 ttgaaaaaag taaataaaat gaatttggag gttgaataat gataaaaaat gcatttgctt
      841 atgtaactag aaaaagttta aaatcattaa ttatcatatt agttatttta tctatgtcaa
      901 ctttaagtct cattagttta tctattaaag atgctacgga cagagcttca aaagaaacat
      961 ttgctaatat aacaaatagt ttttctatgg agataaatag acaagtaaat ccaggaacac
     1021 ctagaggtgg gggaaatgta aaaggtgaag atattaaaaa gatatctcaa acagatagta
     1081 tagactccta tgtaaaaaga ataaacagtg ttgcagattt agttgattat gatattattg
     1141 aaactcaaga gactcttgcg aatcaatcac ctgaaagagc aaagaatttt aaaagaacag
     1201 ttatgttaac tggggttaac gactcaacaa aagaaacaaa atttgtatca gaagcatata
     1261 aattagtaga aggaaagcat ttagaaaatg aagataaaaa taaaatctta atgcataaag
     1321 atttagctga aaaaaataac cttaaagttg gagataagat aaaaataaaa tctaatttat
     1381 ttgatgctga taatgagaaa ggtgcagatg aaacagtaga agtagaaatt aaaggtctat
     1441 tcgatgggca taatagcggt gtagtaagtg cagcacaaga actatacgaa aatacattaa
     1501 tcactgatat tcatacagct gctaaagttt atggtaatac cgaagataca gctgtatacc
     1561 aagatgcaac attctttgta aaaggtgata agaatcttga tagtgtaata aaagatcttg
     1621 ggaaattaga tataaattgg agagaatata atttgattaa aagttcatca aattaccctg
     1681 cattacaaca atctatatca ggtatttact caatttcaaa taaattattt tttggctcat
     1741 taatatttgc aggagttgta gtttcattat tattattctt atggatgaat gctagaaaga
     1801 aagaaatagc agtattacta tcattaggta tatcaaaatt agaaattttt ggtcaattcc
     1861 taattgagat ggtatttata tcaatccccg cattcgttgg ttcctatttc ttagctcaat
     1921 atacagctga caagcttggg aataatatat tgaataaggt tactggtgat atagctaaac
     1981 aaatagctaa acaatctgca tctagccaat taggtggtgg ggcagaagct gaaggattta
     2041 acaagacatt atcaagttta gatatcaatg tattacctaa attcatgatt tatgtagtta
     2101 tatttatgag tttagtactt cttgtatcat tgattatttc ttcattcaat atattaagaa
     2161 ggaatccaaa agaattatta attgacaata attaaaattt tcggtgcttt ttagcaccaa
     2221 aaatgagagt gtaatataaa ccgtgtaagt aaccatcaat cctaggcggg tggttgcttc
     2281 acggttttta tttttctaaa ataagatcaa gaggtgatta cacgatggct agaaagaaaa
     2341 gaaatcctga tgctgagaag ttagctgaat ccattttgaa tacttatcaa cccgaatctg
     2401 tagaggatat gcaagatgtt ttaaaagatg tgtttggtcc cttatttgaa aaaatgcttc
     2461 aaggagaatt gaataaccat ttagggtatg acgcccattc taaagagcca aagaaacatg
     2521 acaatcgacg aaacggttat ggcaataaga cgcttaaaac gagctttggt aaagtaaaca
     2581 ttgatgttcc cagagaccgg gaggcttctt ttgaacctga gttgattcct aatagaaaac
     2641 gagatgtctc cgacatc
//
LOCUS       DAZUJO010000169         2491 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000169 DAZUJO010000000
VERSION     DAZUJO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.178"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..1658)
                     /locus_tag="ACG8OL_002940"
     CDS             complement(366..1658)
                     /locus_tag="ACG8OL_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS4818833.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1932..2192)
                     /locus_tag="ACG8OL_002941"
     CDS             complement(1932..2192)
                     /locus_tag="ACG8OL_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818834.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          844 a          465 c          382 g          800 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaatat aaaaaagtaa aatagaccat aggcaataga
     1981 ttaacaatta atataataca agcaaatatt ctattcattg tatttctgct ttcaaaaagt
     2041 tctaatattg aaagtataat tccacaaatg cctataataa gtatgggaat aagattagaa
     2101 aataacaccc cgagccaatc tgtgaaaatt actatattta ctaatatccc tattaacata
     2161 cctatcatat ttattaaatc aatttttttc attagatatt tctcctttat taaatctgaa
     2221 gtacttatat taaaataatt ctaaactatt tacctcaaaa agtgcaataa attatttctg
     2281 aacaataaaa tgatagtcaa aacaaaaaaa tattgttaat cgttcagagc catccgagag
     2341 tgtaaaatat tttgtgtaaa tgaaaaaatc catacaaaaa aggaaggcgc ttctgtagaa
     2401 taaagttaac gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca
     2461 gaaattgtgc aaactctagt cactaaaggc g
//
LOCUS       DAZUJO010000170         2398 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000170 DAZUJO010000000
VERSION     DAZUJO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.179"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8OL_002942"
     CDS             207..2069
                     /locus_tag="ACG8OL_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS4818835.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ACG8OL_002943"
     CDS             2069..>2398
                     /locus_tag="ACG8OL_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS4818836.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZUJO010000171         2364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000171 DAZUJO010000000
VERSION     DAZUJO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.180"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1451
                     /locus_tag="ACG8OL_002944"
     CDS             150..1451
                     /locus_tag="ACG8OL_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4818837.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="ACG8OL_002945"
     CDS             complement(1555..1755)
                     /locus_tag="ACG8OL_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS4818838.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            2151..2300
                     /locus_tag="ACG8OL_002946"
     CDS             2151..2300
                     /locus_tag="ACG8OL_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818839.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          768 a          453 c          408 g          735 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacacaa ggataatgca
     1921 atgaaatata aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc
     1981 gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta
     2041 aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta
     2101 tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct
     2161 ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt
     2221 ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag
     2281 aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc gttcttctct
     2341 ttaaacgcat ctactggtag ataa
//
LOCUS       DAZUJO010000172         2337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000172 DAZUJO010000000
VERSION     DAZUJO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.181"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..>2337)
                     /locus_tag="ACG8OL_002947"
     CDS             complement(226..>2337)
                     /locus_tag="ACG8OL_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS4818840.1"
                     /translation="LEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLG
                     YLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTA
                     KDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQY
                     SAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQELAVVAERASE
                     LPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYL
                     EKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKR
                     NYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDP
                     GSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELS
                     SNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESP
                     GISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQP
                     HLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAK
                     PLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQD
                     HINLTIAKEIMDAYYDMFMAK"
BASE COUNT          597 a          522 c          425 g          793 t
ORIGIN      
        1 tgagccagaa tgtggttcat tctgactcag atttgatttt gcacaactca tcagtcctta
       61 ttgacaaaga gccacaattt ttgtcacaac cttttttgcc gagtaaacgg tgttcaaaag
      121 ccagtttctc ggtatgaagt caaatttaag caaaaatgtg agaagctatt ttcacaaaat
      181 ttttcttcat actcgaaact aagcggcttt ttcacgacct cttttttatt ttgccatgaa
      241 catatcataa tacgcgtcca ttatttcttt agcaatcgtc aagtttatgt ggtcttgttc
      301 gtctaataag ttcggcaaga caacactgat ggcaatttcc ggattgtctg ttggtccgta
      361 tgccacgata ttactattga ctgtcgcaat atctggatga ccttccgcaa ccgtttctgc
      421 agtccctgtt tttgcggaaa ggtccatttt cgcactggct aatggtttgg ctgttgtgta
      481 aggatcggta ccatggacaa cttggtggaa tccttcacgg aggatcgcca tattttctgc
      541 agaaatattg accgtatttt ctactgttgt gccaatttct ttcttcactt cacccaagtt
      601 tccattttcg tcattgccat agatcgcttt gactaggtgc ggctgtactc gtttcccgcc
      661 attagcgact gttgaagcat attgagctaa ttggattggt gtatatgtgt caaactgtcc
      721 aaatgccaaa tcggtaaagt tacctggagc ataccattct ttgccattat ccgcattgaa
      781 cttcttcata taatcaacag aacgtgagat cccaggagat tcattcggca aatcgatccc
      841 agtcgttgta ccaagaccaa actctttgaa agagtctctc aattgctgat aagctttagc
      901 ttcttcatct aaactaggta gtcccattcc aggtgtatag tctagtccta acatctttaa
      961 cgccactttg atcatatacg tattagaaga caattccgat gctttgactg catcaagagc
     1021 aacttgtccg cttcgattaa agacagagct tttttctgaa gcaccttgca gacgaatcgg
     1081 ctcatcaatc aaaacttgat ttccgctgat cgcgttactt gcccagcctg ctgtcaaagt
     1141 accagccttg actacagacc ctggatcaaa ggcgctagta atcgtgccta aagcattctc
     1201 ggtgatttct ttggaacctt gttcatgact gaaaccagtc attgctagca cttctcctgt
     1261 ctgaggattc atagccactg cataagcacc aggcgaatac tgtgctttgc cgttgttgat
     1321 caatgtttga taattccttt tcaaaatttc ttctactttt gattggaatt ctgcattcat
     1381 agataacatt aaattagaac ctttttcccc agagaagatt tctttttgat tcgaaacatt
     1441 tccttcatta tcaagggaaa cttcatattg ggttttggtt ccttgcagga catcttcata
     1501 ttgtttttcc aaataacttt gtcctacacg gtcattccga gaataacctt ttgctaggta
     1561 tttctctgct tcatctgctg gaagtccttg cttttcagtc gtcacactgc caagaatact
     1621 tttcagtgaa cttgctgcat tatattctcg tgtccagtct gtgccagtcg aaactcctgg
     1681 aagttcagaa gcgcgttctg ccacaacagc taactcttga tcagtaacac cagagttttt
     1741 gatatagaca gtgtttaatt catatgcact attcatccgt ttaaagatgg tagcaatttt
     1801 taattgctcc tcgtttaaat taatctcatc agctgtgact ttattgacca tcgcactata
     1861 ttgttcgctg ttccctaatt gcttttcttt tgccgaaagt ctttttgtgg cttctttcaa
     1921 atgtttggga tcagccagcc aaaaatcttt tttatctcta tctgtcagat tttcatctac
     1981 cggaacatcg atcagcgagt tcagcttcgt tgcgatttca agcaaatctt tagccgtaac
     2041 ttgtgttcct ctcgtatatg tgatggcctg attcgcttta ttcgtaacta aagcttttcc
     2101 tgtggagtcg tagatcattc cgcgaggagt actttcttgg accgtaatcg tagaagtaga
     2161 cttcactcgc gcctccatct cttcgccgtt cacgatctgc aaataaccca atctagcaat
     2221 caacgcaaca aataacgtaa atataatgaa aaaaagaaaa tttaagcgaa aaggaacatg
     2281 ggaccggcga aacgttttgt tcttaggttc ttgtgcacct tttcttattt tttctaa
//
LOCUS       DAZUJO010000173         2314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000173 DAZUJO010000000
VERSION     DAZUJO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.182"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..675)
                     /gene="wzy"
                     /locus_tag="ACG8OL_002948"
     CDS             complement(<1..675)
                     /gene="wzy"
                     /locus_tag="ACG8OL_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFS4818841.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFI"
     gene            complement(694..1773)
                     /locus_tag="ACG8OL_002949"
     CDS             complement(694..1773)
                     /locus_tag="ACG8OL_002949"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4818842.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIY
                     GEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEAL
                     AIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQK
                     GIKLRKELTSKNISNIWYEYLNQFF"
     gene            complement(1787..>2314)
                     /locus_tag="ACG8OL_002950"
     CDS             complement(1787..>2314)
                     /locus_tag="ACG8OL_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4818843.1"
                     /translation="SLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYY
                     RQQNNSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQ
                     KNVGGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFK
                     MYKTAYNMLWRIYNR"
BASE COUNT          765 a          431 c          253 g          865 t
ORIGIN      
        1 aataaaaaca aatctcgtta ttacttttga aaagttgcta ccaattaata aacatattaa
       61 ggctggaaaa aaaagatata gaataatttg taaatatcca ctttgtgtag cataatcacc
      121 ataatataat gcattataac catggactaa tgctatacgt acctctagta atttagaaac
      181 aaaaactatc ggtactacta ttatagaaat gatacaccct attttaaata aaaactttct
      241 atcggcatca gcatctccag attcatacca actaactttt cttagtgtgg tttttctcgt
      301 aaataagagt gctcctatgt gaaatacaaa catagaaata caagaatacc attttaccat
      361 tatcatatct gattcactag gaataatccc cgtaccataa gctactactg ttgtaccgat
      421 tccacgatct gttccaatac cgaatgccca cataatgcat tgcccataac taaataataa
      481 aaaaagagtg aaaaaaatcg tgtataatga aaatattgaa tctccttttc tccaatgcca
      541 tgtacagatt gcatataagt aaatcaatat tccaagccaa tttagccaat agttctgatt
      601 taatgaaaaa gcgcaaaata ttgtaaacgc gactaacgtt aacgaaaaca tcaatatatt
      661 tattttttct ttcacacaat atcctccttt tggttaaaag aactggttta aatattcgta
      721 ccatatattt gaaatatttt tagatgtcag ttcttttctt agtttaattc ctttttgcgc
      781 ttttcttacc acactttctc tatccaaaag taccctacgc attgcatctg ttaacttatc
      841 tagattatta cattcaacaa gatgacctat ttcatttcta tcacctaaat acattcttgc
      901 gccacctact ggacaatctg tagcgataac tggcactcca atagccagtg cttctaacat
      961 agaattagat ataccttcat aatcagaaga aattacaaat cctgccgcat tttgcatctt
     1021 ttcatgtatt ttatttgtaa attcttttag atgaacaaat tctgttaatc cattatggtt
     1081 gatataactt tcaagctcgg tttttaatcc cccttctcca taaatatcca gttcacactc
     1141 atatccttca tgaataagtt ttcctattgc atcaatcatt aagggcaaat ttttttgttt
     1201 atccaatcta catgcagtaa taaatttttt actacttgta gttttattat aataaggtaa
     1261 attttccttt attgggttgg gaattataat tgtttttact tttttatcat tgatcttact
     1321 aaaatatttt tttgcgtctg gtgtttgaaa aacaattcca tctacatttt tataaagaaa
     1381 atctctaatt ttcctaatcc atggttcttc tggattccgt tgtggatcat ttcgttcaga
     1441 aacaattaat ttttgtttta ctcctaacaa tgaaataatg acatggatat ttacatcagc
     1501 aaggaacgaa ataaccagat caggctgtat ttcccttatt tttttcctaa gttccttaaa
     1561 gcgttccaaa ggtctaaatg cacgaaacga acttttacat attattggat gtacctgtat
     1621 ttttctattc agttcatagt cttgccgatt cccatatata gttataatat caatttccca
     1681 gttatcttga ataaaattat ttgccaaatt cgcaattact cgttctgccc ctccttttcc
     1741 cataattcct atcacaaagg tcaatttcct catatttcct acctctttat cgattatata
     1801 ttctccagag catattatac gctgttttat acattttaaa attaaacatt atactaatgg
     1861 tcacaagggc taacttcttt ctcatattag gttcaagttc atatgctttc attctaacta
     1921 tatttgaaat attttctctc aatattttat gatgtttcaa agaaactaat gcattcttat
     1981 aatcttcaca tgttccacca acgttttttt gattaaaaat ctccataatt agtacatcaa
     2041 atgcaaccaa catacaagct tttttatcta ttatgttgtt tccattttgc cttatcaact
     2101 tttcataatc agcaatcata tttccatgtc taaaaaccaa attacttttt ggcttatgca
     2161 ttatagaatt attttgttgg cgataataat aacacaattt atctacgaaa cagattcttg
     2221 tatttgtcat aaatacttgt aattgaaacc aattatcttc tgcaaaaatt attttgttat
     2281 caccaaatga taaattaaac tctaatacta aact
//
LOCUS       DAZUJO010000174         2211 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000174 DAZUJO010000000
VERSION     DAZUJO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.183"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..715
                     /gene="wzy"
                     /locus_tag="ACG8OL_002951"
     CDS             <1..715
                     /gene="wzy"
                     /locus_tag="ACG8OL_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFS4818844.1"
                     /translation="GDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIGIYILN
                     VITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNGIYPFA
                     NTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNGGYIGG
                     IVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIVKNFVY
                     GVLIILLINFFLQQFNKRL"
     gene            761..>2211
                     /locus_tag="ACG8OL_002952"
     CDS             761..>2211
                     /locus_tag="ACG8OL_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS4818845.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWVRKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNVLFY"
BASE COUNT          716 a          301 c          388 g          806 t
ORIGIN      
        1 aggagacaga ggaagttggt tgtatagctc tattatactc atatgggcat acactcaata
       61 taaaggaacc tttaattata aaaaattaat aaagtggctt attttagctg ttataggaat
      121 atatattcta aacgtaataa cgaaagcacg tgatggtgga ggactaagta aacttacttt
      181 aaaagatttt acatctgtat ttgattcaga tgattctcct ttagttgatt ctttttttga
      241 aatggggggg actatgagta ttataacttt tttcctaata agtggtaatg gaatatatcc
      301 ctttgctaat acttatttga cctcactctt aggctctata tcaacaagaa tgctatcaat
      361 gtttggaata aagtttgttt tattagctga ttggttttct caggattatt taggaatcac
      421 ttgggggact ggattctcaa tgattgcaga agcctatgtc aatggtggat acattggggg
      481 tatagtctat atttttatta ttggtatagt catcggtaaa atcctttcta atagtataga
      541 caaccaagat attgaaaatc atcctatgaa gatgtttttg gctataacta cagcaaatat
      601 tataatgggt tttcctagag ctgcttcata tttaattgtg aaaaattttg tgtatggtgt
      661 tttaattata ttgttgataa actttttcct acagcaattt aataaacgtc tttaatattt
      721 aagcttaata aaaagataaa acaagtcagg agttgcaaaa atggaaagaa ctgctcggct
      781 caaaaaaaac actatttggg ctctggtgtt tcaaataact tctattgtct gtggtatgat
      841 cttgctacgt ttgatattaa caaagtacgg atccgaggtt aatggaatga ttagctctat
      901 taaccaattt ctacaaatta tttctttttt acaattagga gtaggcgctg taatgcaatc
      961 aactctatac aaaccactag cagaaaagga taatttaatg ataagtcaaa tcttagcttc
     1021 tggagggaag ttttatcggc aaattgcata tattctacta gtatacgtgg gtttgttact
     1081 tgcagtctat tatgttctcg ttcctaatga atttgggaga tcatatacaa tggcaatgat
     1141 tgcaacatta agtattaatt catttgctca atattatttt ggtattgtta atactttgtt
     1201 attaagtgct gaccagaaaa tttatattac gtatattgca cagacaatag catttatttc
     1261 taatacagtt gcatgtgctt tattaattag tctgaacaca gagatcctta tggtaaaact
     1321 tattactgct ataatttatc ttgcttgtcc aatatattat agatggtatg tggatcgaaa
     1381 ctataatata aagaggaatg tgtcatatga taaggaaccg atccctcaaa aatggaatgg
     1441 ggttgcacaa cacgtagctt ctgttgtact agatagtact gatgttgtag tgttatctat
     1501 attttctaac ctgatatatg tatctatata taatgtgtac aatttagtaa tatacggagt
     1561 aaaacagcta ttcatggcta tgacaaatgg cgttcaggcc ttactagggg aactttgggt
     1621 cagaaaagaa atagaagttc tttacaaaac atttgcatgg ttcgaatgga ttgtacatac
     1681 tttgacagtt tttatttttg gctgtaccgg tatactaatc gttccattta ttcaagtata
     1741 tacaagagga gtaacagatg ctaattataa tcaattatca ttcgcagctt tacttgtaac
     1801 tgctaacgca cttcactgtc tgcgtacacc ttatcatgta atgataaaag cagctggtaa
     1861 atataaagaa actcaaaata gttttataat tgcttctttg ttaaatttag gtttatcatt
     1921 aattttggtt aaaccactag ggctagttgg agtagcatta ggatctctaa tttcaatcac
     1981 atatcataca ctttggagtg caaattataa ttcaaaatat ttgcttaaat ggccgatgaa
     2041 aaaattctat aaacagttat ttgtaaacat cttaactgta gttctttctt ttggggcgac
     2101 atcacaattg aaaatgatgt caatcacata tttggattgg attgttctcg caattaaatg
     2161 tgcagttatt gtatttatat gtataatcct agttaatgtt ttattttata g
//
LOCUS       DAZUJO010000175         2135 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000175 DAZUJO010000000
VERSION     DAZUJO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.184"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1024..2037
                     /locus_tag="ACG8OL_002953"
     CDS             1024..2037
                     /locus_tag="ACG8OL_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS4818846.1"
                     /translation="MTKKNRVTIRDVAQAAGVSISTVSKALNSLDGVSPETRVEIQNI
                     ASRLNYVPNLMGKQLKSGKTKMIGVYTNRISGPYFNSLIDEIAFESKLHGYGLNVIVS
                     DERDVIMSSLLGNFVDGAIVVEDVLNQKDIETINSNGVATVFINRIVADKKVGSVAFD
                     SFEKGYLAGNYLLNLGHKKIGYVNGVDDIYDNDQRFQGFKAALSKVGLTYNEDFTIAG
                     NFVEEDAYANTLAFIDNQPAELPSAFLAANDMSAVGLIKALTEKNYSVPEDFSVVGFD
                     DVEVLKYFKPSLTTIHNSTKEQGKKAIEHLLEMMGGKSVGRVIEVKGTLMIRETAIEY
                     TGE"
BASE COUNT          700 a          311 c          435 g          689 t
ORIGIN      
        1 gacaaaaaga aagatctttt gcccatttta tttttataaa atgggtaggt ggcgttcgcg
       61 taaagcaaat cgatacaatc caatggtttt ggcgaatcag atttaccatt ctaggcttgt
      121 ttaatacaga catcgattgt tattttgaaa atttatatcc ttattggcaa tgaaatggat
      181 attctccata tcaatagaaa tcttcaagat aattttcgga aatatataag agttaatcaa
      241 ttatgatcat atagtaggta aaaattagga cagccttcaa ttatatggga aaccaatata
      301 atcaagtaat tattcgggta acacaaatac tagagtactc atttcttata actgttttac
      361 acctaaaaat gagcttatcg gaaactttgc aacagaacca gttagctgtt acatttaaga
      421 taatttcaaa atcttgatct attaattaga aagtaggggg taaggtgggg aaaaagaatt
      481 ttgttttatt tttatttgaa acgatggtcc agctttggtc gttattttta tttctgttaa
      541 ttatttataa atactttttt ggaactagtt tttcttttat tacgatcact cggccagaat
      601 tttatttaac gggtttagtt agtcttgttt ctacgatcgt tttatatttt gtccgatcgc
      661 gaaagtaaac aaataaattc ttaaagccaa ctcccttttt gtaaggagtt ggcttcttac
      721 tttcctccgc ccacaagttc atttacctaa cgtgtgaaaa acattcccat aatttctaat
      781 taaataattt atttttatta taaaaacgtt ttccttttct ggtaaaagag caggcgttag
      841 aaatccccaa gctaaaaggt ttttccctga ttcaaacacg aaaaacaaat gtatacgtta
      901 acaaaggcgt taaattagct tttatttttt ataaaaaaag cttgtatcca aaaaaataca
      961 gtgttataat tcaatcgaag taaagaaaaa cgttttcttt aaaaagtaca tgaagtgaat
     1021 aaaatgacaa aaaaaaatcg ggtaacgatt agggatgtgg cacaagcagc tggcgtttca
     1081 atctcgacgg tttccaaagc gttaaacagt ttggatggcg tttcacctga aacccgggtc
     1141 gaaattcaaa atattgccag ccgtttaaac tatgtgccca acttaatggg gaaacagtta
     1201 aagtctggta aaacgaagat gatcggggtt tatacgaatc ggatcagcgg tccttatttt
     1261 aactcactaa ttgatgagat tgcttttgag tctaagttac atggatatgg tttaaatgta
     1321 attgtctctg atgagcggga tgtaattatg agtagtctgt taggtaactt tgttgatggg
     1381 gcgattgtgg tggaggatgt cttaaatcag aaagacattg aaaccattaa ttccaacggc
     1441 gtggcgacgg tttttattaa ccgcatcgta gcggataaaa aagtcggcag tgtggccttt
     1501 gattcgtttg aaaaaggtta tctggcgggg aattatttat taaacttagg tcacaaaaaa
     1561 attggttatg tcaacggggt cgatgatatt tacgataacg accaacgttt tcaaggattt
     1621 aaagcagcct tatcaaaagt cggtttgact tataatgagg actttacgat tgccggtaac
     1681 tttgtagaag aagatgccta tgccaatacg ttagccttta ttgacaatca accagcagag
     1741 ttaccgtcgg cctttttagc ggcgaatgat atgagtgcgg tggggttgat taaagcctta
     1801 actgaaaaaa attattctgt accggaagat tttagtgttg ttggttttga tgatgtggaa
     1861 gttttgaaat atttcaaacc aagcttaaca acgattcata attcaacgaa ggaacaaggt
     1921 aaaaaagcga ttgaacattt attagagatg atgggtggga aatcagtcgg tcgtgtgatt
     1981 gaagttaagg gcacgctgat gattcgagaa acagcaatag aatacacagg agagtgattg
     2041 agtatgtacg ttgggattga cgttgggggc acaagtatta aatacggttt agttgattca
     2101 aaaggaaaca tcgtggccaa agatacgtta gtcac
//
LOCUS       DAZUJO010000176         2070 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000176 DAZUJO010000000
VERSION     DAZUJO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.185"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..735)
                     /locus_tag="ACG8OL_002954"
                     /pseudo
     CDS             complement(205..735)
                     /locus_tag="ACG8OL_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            807..>2070
                     /locus_tag="ACG8OL_002955"
     CDS             807..>2070
                     /locus_tag="ACG8OL_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFS4818847.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMI"
BASE COUNT          659 a          400 c          368 g          643 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac taacatttcc atttcattta
      121 cctctttttc gtttaattat actaaagttc tatccaagcg acaagctgtg tataataaaa
      181 atatacccgt ggtgctagtt aattttaata ccctaaagaa tatctcaaag tcccttcaaa
      241 atgttgctgt gctcggctta aaagaatact gtacgataat aatatgcttt caaacttact
      301 ttctagccct tgaatagaac gtgatttgaa attttgaaaa cctagtcttt cgagagaaga
      361 aaaaacagtt tctactgctt ttctttgtct agtaagtaat cgatggtcta tcttccctag
      421 tttatccatg ttttttcgtg acggaactaa gactgtaatg cccttaaggg caagcttttc
      481 atggatttta tggctgacat agcctttatc tccaaggata tttggaaggt ttgcttcttc
      541 cgataaagtc tctaaaactt tcacatcatg aacactagga tccgtgattg aataagtaac
      601 tggaaagcct gttttagtga cgataatatg aattttaaga ccataaaaat aagcgttttt
      661 cgttgagtta tagcccctac agctctatac tttttgatgt tggtggatga caccagcatc
      721 ttttttattt tccaaaacat atagaaaaga caccttgaaa tcctttagaa ttaatttggc
      781 gaaccaaccc aaaaggagga atcaagatgt cttacactca ccttatcaaa gaaacattag
      841 atattctaga cttatcagtt acttttaacg aaaattgtct cacaaaagaa aaatacaaag
      901 ggcaaatctg tcatatttat cgtggaaact tgatttatac agcccaagaa tgcatccact
      961 gtaaacacca gattgcttca gatattgtgc gttggggtac aacaaccgtc cggttgttaa
     1021 tgaacgatgt ttctgaatgc cgtacctacc tcgaattgaa aaagcaacgc ttcaaatgca
     1081 aagcttgtca gcggaccttt gtcgcagata cttctgttgc gaaaaaacac tgtttcatca
     1141 gtgagaaagt ccgctggtct gtcgtgactc gtctgaaaaa aaatacctca atgacagaga
     1201 tcgccgctca gaaaaatcta tcggtctctt ctgtttaccg tattatgaag cgattttatc
     1261 gtccgttgaa tccttttcgg acaccgttac ctaaggtact ttgttttgat gaattcaaat
     1321 cagttcgggg tgtttcaggt gctatgagtt tcatcatgat ggatggacaa acacagcgct
     1381 tgttggacat tgtcgagaac cgacaactgc cttttttaaa acgttatttt agtcattttt
     1441 cacgagaaat tcgtgaagcg gtcgaatgga tcgtgattga tatgtacgct ccttatgtat
     1501 cgctcgttaa aaagctgttt ccaaaagcac agctgattat tgaccggttt cacatcgtcc
     1561 aacatattgg tcgtaccttt cgcaatcatc gaatcaaaga aacgaaccaa ctattaaaaa
     1621 gcaaagaaca aaaacactat caattaggta agcaattaaa aagatactgg aaacttcttc
     1681 aaaaagatga acgcaagctt gattatactc gacgcctttg gcgacctggc ttcaaagctc
     1741 atttaacaga gaccgatatt gtggatcgct tactgaaagg gagccctgct ttgcgtgtag
     1801 gctatcagtt atatcaagac tttctttacg ctgtaaagga acgagactac gtatcgtttg
     1861 aagaactact taccaataat atcatgcttc ccgaaggcta tcagacgacc ttgagaacgt
     1921 tccagaaatt tctgccacaa atcaaaaatg ctttacaaca aagttattca aatggcccac
     1981 ttgaatgtct gaacaaccat atcaaagtgt taaaacgaaa tgcatatggc tttcgtagct
     2041 tttataattt caaattgcgc atcatgattc
//
LOCUS       DAZUJO010000177         2052 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000177 DAZUJO010000000
VERSION     DAZUJO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.186"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..796
                     /locus_tag="ACG8OL_002956"
     CDS             <1..796
                     /locus_tag="ACG8OL_002956"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4818848.1"
                     /translation="GLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRLAAKKSK
                     VIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLFIGRIMK
                     EKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQSDIRPF
                     VERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCERKNSCS
                     LYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            803..1198
                     /locus_tag="ACG8OL_002957"
     CDS             803..1198
                     /locus_tag="ACG8OL_002957"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4818849.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1195..>2052
                     /locus_tag="ACG8OL_002958"
     CDS             1195..>2052
                     /locus_tag="ACG8OL_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4818850.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKTGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKDKIINLIYPKLIEDEHGE
                     YFRPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDII"
BASE COUNT          807 a          224 c          401 g          620 t
ORIGIN      
        1 tggattggca tgtcggttaa gaaaggtttc ttatgcatta aatataaccg gattaggaac
       61 ggcatttcaa aaaaaggggg ttttgaaaag tcttattgta tttttgtata gattagcagc
      121 taaaaaatct aaggtcatat ttttcgaaaa ttttgaaaat atgaaaacat ttcagacttt
      181 acaaattgct aaacaaagta aatacaagct attaaacggt gcaggagtag atgtagaaca
      241 ctttagttat ttagaatatc ctaaagatga gcaagttcat tttttattta ttggaagaat
      301 tatgaaagag aaaggtgtag atgaactttt tgaagcaatg aaaaacttat atagtgaaga
      361 gaaaaattgt gtacttgatg tccttgggta ttttgaagaa gattacggca taaaaattga
      421 aaaatacgaa aaagagggat ggttaaaata tcatggatat caatctgata ttcgtccctt
      481 tgtagaaaga gcacattgct ttgttttgcc tagttggcac gaaggaatgg caaatactaa
      541 ccttgaatca gcagcatcag ggagaccaat tattacaagt gatatcccag ggtgtaaaga
      601 agcagttatt gaatcgaagt ctggttattt atgtgaaaga aaaaatagtt gtagtctcta
      661 taattcgatg aataaatttc taaaattatc atatgagcag cgcattaaca tgggaaaaat
      721 ggggagaaat tatatgatta aaaaatttaa taagaagaaa gttgtcgatg aaacaattaa
      781 atcattattt agttaaaaag atatgttttg caaagtatgt gatagaaaag agggattctg
      841 tggcttcaga atagatatca aagaattggc aaaagtttct gatatttttt tatttactac
      901 attacaagaa ggaatgccaa gatctatgat ggaagctatg gcaagtggat tgccatgtat
      961 agcaagtaaa atccgaggta atgtagacct tttagtggaa ggaaaaggtg gatatcttgt
     1021 tccagctaaa gatgcagaat tagtagcaga gtgtttgaga gaactaatca ataataaatc
     1081 tcttagaaag tatatgggaa atgagaacct ttccagaatt aaagaattcg aggttagtat
     1141 cgtaaaaaaa agaatcaaag aaatatatga agaagttata ggtgagagtg taaaatgaaa
     1201 aatccaatgt tttcaataat agtacctatc tattgcgttg aaaaatattt gaaagattgc
     1261 ttagattcaa taagaaatca gacatttaaa aattttgaag caatactagt tgatgacggt
     1321 agtcctgacg aatgcccaga aatatgtgac tattattccc aaatagatgg acgtttaaaa
     1381 gtaattcata agaaaaacgg tggaataata gatgcacgga aaacaggagt cactagtgct
     1441 atgggagagt atatagtttt tctagatggt gatgattggc ttgcaaatac ttatttagaa
     1501 aaatatgcgg aagtaattac taagtacaat ccagatataa tatgtagtgg gtattttgca
     1561 tcacgttcca acaaaattag aaaaagagaa ttgtcttgct cgtttggata ttatgacaaa
     1621 gataaaatta taaatttgat ttatcctaaa ttaattgagg atgaacatgg agagtatttt
     1681 agaccacaat tatgggcaaa agcttttaga actactctgt acaaggaaca acaacaagta
     1741 tctgggcaag ttagtgttgg tgaagatcat gcatgtacaa aaccatgtat ttaccatgca
     1801 aattcgatat atatgatgga aaaatgttta tattattaca gaataaacga aaattctata
     1861 acaaaaaaac cacaaccata ttattgggaa ggaccaaagg ctattggaga gcattttgaa
     1921 aagcagattg atatgaataa gtatgatttt caagatcaag tatatagaaa tgttacacac
     1981 aatttattta atgttgctgt ttcacaattt aataaacagg acaaatattt aaatataaaa
     2041 aaagatatta ta
//
LOCUS       DAZUJO010000178         1979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000178 DAZUJO010000000
VERSION     DAZUJO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.187"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..367
                     /locus_tag="ACG8OL_002959"
     CDS             <1..367
                     /locus_tag="ACG8OL_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS4818851.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            567..749
                     /locus_tag="ACG8OL_002960"
                     /pseudo
     CDS             567..749
                     /locus_tag="ACG8OL_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1330..>1979
                     /locus_tag="ACG8OL_002961"
     CDS             1330..>1979
                     /locus_tag="ACG8OL_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4818852.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          657 a          305 c          461 g          556 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttac tctttgtcaa
      601 agagtaacag aaaggtttac actcttttta ttagcgacag cgcgaaggag gggtttaact
      661 tggggtttag tatttcgttc aaaaagggaa caaagaatac aagcatttct cataataatc
      721 gtgagttaac tgatagccaa aaaatcaatg gtttgtctgg tagaaaataa ttagcagatt
      781 tacaagtggg aaataaaaag ctgaaagaag aaaattgcat ttcaaactac attttctaca
      841 atagctctaa aatggcatat tttgcgttct gagcggtttt agcgtttatt tcgtttagtt
      901 atcggcacaa tcgttaaaac acgttagagg agcgaaaatg gagcctgtaa ggcgactggt
      961 agcggaacga tcgtgttaga ttgtgaaaac cgatagctga atgaaataaa cagcgggtgc
     1021 gccctttcca tttcggcagg aggaggctca aggggttcgg ggaatgaaat tccctgaggt
     1081 ctttttattt ttttaggagg agtgctataa tcaaagaaaa cgagaaaaaa gagcaaaata
     1141 aaagccgagc agtgcgctaa cactctcgac tatttacgga aactacttca cctagctccg
     1201 taactcaggt tcattatacg aatattttta ggtttcttca agctagagat ttttggatat
     1261 gaatttttag ggttacgtga actctggaaa ttctttccaa aagtctcttt tttcgtctgg
     1321 aggacgaaaa tggcaaatga aaatcagttg agtgaaattc aatcaaaaat tgaaggtcga
     1381 ggggcagaaa aaagaacaac gataaatcag ctcgaaaaac ttaaccgtat ttctaacagg
     1441 cggttaagtg ttgacgataa ggtacacccc ctaaaattaa gttggtcaat cgatcgaatt
     1501 acaatcgtcg gaaaactgaa tgataccata gcttattgga cgcaaaatgg agttgtgttt
     1561 gtcgattttg agcaattaat gcgtttgaat gaaaatcaag ggtatttgaa gtcggttggt
     1621 acaaatggtt ggcaattatt tgaccaatat ggagaagata tcgcctatat cgaaatgtta
     1681 aaatttcacc ctgacaaagg tcgtattgat tttaatccaa ataaaattgg cgagttttta
     1741 aaggggtcga tgaaacaatt cattcatgat ttgtttctgg atgcgcattt ctcacgtgca
     1801 gatattgctt gcgatattct cggtgttcct gatgatttta ttacccagta tcgaatcgtt
     1861 gaccctgtgt cttttaagcc aatttatggc cgttctggca aattagaaac agcgtactgg
     1921 ggtagtcgtg ccagcgagcg acaagtgcgg atgtacaaca aaaagcttga acaggaacg
//
LOCUS       DAZUJO010000179         1942 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000179 DAZUJO010000000
VERSION     DAZUJO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.188"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="ACG8OL_002962"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="ACG8OL_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS4818853.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="ACG8OL_002963"
     CDS             851..1039
                     /locus_tag="ACG8OL_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS4818854.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="ACG8OL_002964"
     CDS             1052..1609
                     /locus_tag="ACG8OL_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS4818855.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="ACG8OL_002965"
     CDS             1641..1883
                     /locus_tag="ACG8OL_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS4818856.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          714 a          301 c          400 g          527 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1921 aatttgagaa ccgtagcgac ac
//
LOCUS       DAZUJO010000180         1881 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000180 DAZUJO010000000
VERSION     DAZUJO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.189"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..685
                     /locus_tag="ACG8OL_002966"
     CDS             <1..685
                     /locus_tag="ACG8OL_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818857.1"
                     /translation="KSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVKN
                     IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSI
                     FSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPS
                     MMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAEL
                     RKLVEYGII"
     gene            672..>1881
                     /locus_tag="ACG8OL_002967"
     CDS             672..>1881
                     /locus_tag="ACG8OL_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS4818858.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFH"
BASE COUNT          631 a          239 c          294 g          717 t
ORIGIN      
        1 gaagagtata ttttcaacaa gtaaaaacgt tcaaagagtt tttaaaatat taaataaaaa
       61 tagcatcttc aaaaaaaaaa ttgacgatta ttttgtctat tgtccggaat tgttacttta
      121 tgatgtaaaa aatataaaaa aaattcccaa aatagatgaa aacagtaaat tgatgaaaaa
      181 catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta tcttttttga
      241 tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg ctaaagtaaa
      301 tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag caaggacaaa
      361 tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct tgctacttga
      421 aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt tttcaccatc
      481 tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt taaatgaata
      541 tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt tctgtgaaca
      601 ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag aattaagaaa
      661 gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt aagtccagtt
      721 tctggtatac tatagggaat ttttttatta agggaatttc attcataact attcctattt
      781 ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact tcatttgtat
      841 caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat gcaagttttg
      901 actataaaga tgacataaat gaatttatgt catcggtact ttctctagct acactttcgt
      961 taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct ttttttgata
     1021 tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt gtaattagtt
     1081 atatccagtc ttattttacg attaacttta accattttaa atatttgatt ttatctttta
     1141 tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta tattttgatg
     1201 ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata ggtttagtaa
     1261 tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag tggagatacg
     1321 ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt ttagcacaat
     1381 tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata tattcctaca
     1441 ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac gcctgggttc
     1501 catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa gttagtaact
     1561 attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg attgatttag
     1621 caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt ccgataacct
     1681 taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt tatgagaaaa
     1741 aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata ctattaaaca
     1801 tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta tttacgtatt
     1861 ttttactttt tatttttcat t
//
LOCUS       DAZUJO010000181         1854 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000181 DAZUJO010000000
VERSION     DAZUJO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.190"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8OL_002968"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8OL_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS4818859.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8OL_002969"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8OL_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS4818860.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          550 a          329 c          300 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DAZUJO010000182         1744 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000182 DAZUJO010000000
VERSION     DAZUJO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.191"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(640..1038)
                     /locus_tag="ACG8OL_002970"
     CDS             complement(640..1038)
                     /locus_tag="ACG8OL_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS4818861.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(1035..1271)
                     /locus_tag="ACG8OL_002971"
     CDS             complement(1035..1271)
                     /locus_tag="ACG8OL_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS4818862.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(1431..>1744)
                     /locus_tag="ACG8OL_002972"
     CDS             complement(1431..>1744)
                     /locus_tag="ACG8OL_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818863.1"
                     /translation="KVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSP
                     TLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYF
                     G"
BASE COUNT          591 a          284 c          248 g          621 t
ORIGIN      
        1 taaaactgct taacagctga aatagctgtt aagcagtttt ttattgatat ctaataggta
       61 agcaaataaa tagaatggtt aaaattaatc ggtccgaaaa tgtatccgta catatgaaca
      121 tatgaaattt aatctgttga ttacaataag cgaatcgtta tcaatgttta tctgtggtta
      181 attaaaaaat ccttccgcaa cggcaaatac aatgattcat atctaattcg ttcatatttg
      241 attaactgaa taaagaaact ggtttttatc attcaattaa acgacaaaaa aagtaagaaa
      301 aactgcaaaa tttttttata attttgcata agcagatttt gatggaaaaa tgacgatttt
      361 tgataaaaaa ccgttcgaga gaggtttatc attttttaaa tctactttgt ataatatata
      421 ttatgtaaac taaaatatat aaaaaatcag cccactagtt ctaaaccact atttcctact
      481 attcactctt ttgtattcct tttaaatcaa ttatttcaaa attaatccat attttccata
      541 tttttatgaa aaaactattt tgtatgatta aacataaaaa aatcagcata ttccttaatg
      601 acatgctgat ctaattgaga aataaagttt ttaattcgtt cattttattg cggtttgtcg
      661 taaataactg gttatcttgg actttagtga atcgaaatcc aatgaactag tggtaatcat
      721 tatgataaaa tcaacagctt cttgacgaga aaaagcaact ggacaattat tcaattgaag
      781 aaacaacaac atggcaacaa gagcagttcg tttattgcca ttatgaaaag gatgtttttt
      841 agctaaatta attgctaaaa tcgccgcttt ttcatagatt tcaggataga gttcttgatt
      901 gaatactgct tgtgaaggct gtttgacggc catatctaat gcatttgcat cttttacacc
      961 gatcatttcg cctggcgagt gacgtaaaat aactttcgcg ttgattttga taatttcttt
     1021 agtcgaaaga tacctcataa atcaaccaaa tcttttaacg cgtcttcata ttgattcatg
     1081 atcgaatcaa ctaatgaatc aatatcaggt tcttcttgtg ctttgatcat tcgtacagaa
     1141 ccatcagatt gaaaaacata actgattgcg tcacctgtct gcacacccat gaattctttt
     1201 acttctggtg ggatagttgt tacatcgcta ttgccagatt ttctaatttt atactttttc
     1261 atttttttca tatttcgcca ccctctatac cattctcatt ctatagtaag tacattatat
     1321 ccaagtatat acttttaatc aatgtatata cttttatttt gctttttctt acgaaaaaat
     1381 gacaaagaaa aaagtttgtg acatgacttt tgtcacaaac tctcgtattc ttaaccaaaa
     1441 tattgatgaa atacttgtgt aataactggt agaaaataaa taatgatcat agccgctacc
     1501 accaccaaca attgttttcg ctgtttcttt cgctttagca tgcttgtcgt tttatgaaaa
     1561 aaataagtag cgcttttagc tcctgcagtc aaagtcgata aaatcaacgt tggtgaatat
     1621 gctgtttgat tgtgcgtcaa tgacaacaac ttcgtcccgt aatcaggata ctttagaaat
     1681 gcttggtaca cagccacaat cgtgattgct cccataagat aatttaaaaa tgaatgcact
     1741 tttt
//
LOCUS       DAZUJO010000183         1718 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000183 DAZUJO010000000
VERSION     DAZUJO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.192"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..585
                     /locus_tag="ACG8OL_002973"
     CDS             19..585
                     /locus_tag="ACG8OL_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4818864.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            730..817
                     /locus_tag="ACG8OL_002974"
     tRNA            730..817
                     /locus_tag="ACG8OL_002974"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:762..764,aa:Ser,seq:cga)
     gene            1151..>1718
                     /locus_tag="ACG8OL_002975"
     CDS             1151..>1718
                     /locus_tag="ACG8OL_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4818865.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSN"
BASE COUNT          581 a          267 c          339 g          531 t
ORIGIN      
        1 agaagtgggg gagttttaat gagaagaaaa gtctatacca aggatcaaat tctaaaagct
       61 gcttatgatg ttgttgcaaa ggaagggttt aaaggattta ctgcgagaaa tattgctaaa
      121 aagatgggga tttcgacaca gccgatttat ctggaattca aaaacatgga agatttaaaa
      181 aatacgttgc tggattctat ctttgaacaa ctaaaagaag atgttttccc tattgaacgt
      241 actggtgata aaatcattga tctcggtttg aactatatct actttgcaaa aaagcatcat
      301 agtctttaca tggcattgta cattgatgaa catggggacg gaaagaaaat gcatgatttt
      361 tcatacaatt atttccgtga aatagttaaa gaagatgaaa gatacaatca attatcggat
      421 gaatatgtcg atgctctgca tactggtaca tgggtagcag tgactggctt agcgtcattg
      481 atgtcatcaa acatcatgca tccaacagaa gaacaaatca ttgcactgat caaacggaca
      541 attgactcga ttttagcatt agataatcct gaaataatca gatagtgttc tatcagtatg
      601 aatttgatcg gtaagtccgt tggctgcaaa ctgtcaatgg acttttttgc taaaaaaagc
      661 gatttctgtt atttatctat tagaaatagg cttgacattt cgtgaaaaaa gtttaaaaat
      721 agtacttacg gaaggttggc agagtggtaa tgcaccggac tcgaaatccg gcgaaccggc
      781 taataccggc gcgcaggttc aaatcctgta ccttccttat atcaacgatt agaggttgtg
      841 aagaagctgt cctgcatcaa gtaataagaa cggccaaaca aaaatagctt ttcctatttt
      901 ttcgtttgat tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaata
      961 aggggctgtg acaagacttt tgtcacagcc ccttattgtt gtacttttaa cctacaatat
     1021 tttttgattc cccctgagta ctatttttta taaatgctag taaatcaatc ttctttggcc
     1081 tttttttgat aaaaaaaata ctagatgtaa gataaaagtg taaaaacgat ttgttttagg
     1141 aggggaaacg atgtgtaccg caatcactta tgcaacgaaa gaccattatt ttggaaggaa
     1201 ttttgattat gagatgtctt ataatgaagt ggtcactatt actccaagaa attatcgttt
     1261 cgattttaga aaggtaaaaa atttagataa acattatgca atgataggaa tagcagctgg
     1321 ggtatccaac tatccactct attatgaggc tacaaatgaa aaagggttga gtatggcagg
     1381 actgaatttt cctggcaatg ctgattataa agaattacaa gaaggaaaag ataacgttgc
     1441 accatttgag tttattccat ggatactagg gcaatgctca accatagacg aagctaaaga
     1501 attattggct actatcaatc tagtcaatat cgattttagt gaaaaactac ccttgtcacc
     1561 tttacactgg ttattagctg ataaagaaaa atcgatagta attgaaagta tgaaagatgg
     1621 acttcattta tatgataatc ctgtcggtgt gttgacaaat aatccgccat ttgattatca
     1681 attatttaat ctgaacaatt atcgctcgct atcaaatg
//
LOCUS       DAZUJO010000184         1680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000184 DAZUJO010000000
VERSION     DAZUJO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.193"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG8OL_002976"
     CDS             64..972
                     /locus_tag="ACG8OL_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS4818866.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACG8OL_002977"
     CDS             972..1592
                     /locus_tag="ACG8OL_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818867.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          602 a          246 c          377 g          455 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
//
LOCUS       DAZUJO010000185         1677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000185 DAZUJO010000000
VERSION     DAZUJO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.194"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG8OL_002978"
     CDS             64..972
                     /locus_tag="ACG8OL_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFS4818868.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1589
                     /locus_tag="ACG8OL_002979"
     CDS             972..1589
                     /locus_tag="ACG8OL_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818869.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          620 a          226 c          348 g          483 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1501 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1561 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     1621 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaa
//
LOCUS       DAZUJO010000186         1664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000186 DAZUJO010000000
VERSION     DAZUJO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.195"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8OL_002980"
     CDS             131..505
                     /locus_tag="ACG8OL_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS4818870.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8OL_002981"
     CDS             complement(619..1248)
                     /locus_tag="ACG8OL_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS4818871.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8OL_002982"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8OL_002982"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZUJO010000187         1654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000187 DAZUJO010000000
VERSION     DAZUJO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.196"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..>1654
                     /locus_tag="ACG8OL_002983"
     CDS             275..>1654
                     /locus_tag="ACG8OL_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818872.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQ"
BASE COUNT          599 a          230 c          297 g          528 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acag
//
LOCUS       DAZUJO010000188         1639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000188 DAZUJO010000000
VERSION     DAZUJO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.197"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            415..>1639
                     /gene="ltrA"
                     /locus_tag="ACG8OL_002984"
     CDS             415..>1639
                     /gene="ltrA"
                     /locus_tag="ACG8OL_002984"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS4818873.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRL"
BASE COUNT          585 a          299 c          388 g          367 t
ORIGIN      
        1 acttggtggt gaaagtccac tacaggcttg gcagtaggaa ctgttagcga aagacaaggc
       61 ggcaatcgtg aggttgtggc tgaaggaagt cggacgcaaa ctcctgaact gacgaacaga
      121 aactagatac aaggctgaat taggtcggat aagtcttcat cacaagacaa agtccaatac
      181 tgcccgaaac ctatacagta aatctagcag ttacatggga ggaaagtttt cactcttacc
      241 gggggagatc tcatgagggt gttgtgggcg ttgaatgaaa acaaaccagc acaacgatca
      301 tggcgacatg atggtgaatc atgagaagtc agccgaggtc atagtagtaa cgacactgac
      361 cgttacgaag gactgaacaa taagaatctt gaataattta ggaggtgtga gaagatgcga
      421 cgaccgcaga aaacaagaaa acttggctat catcaaagag ctagtgtgga acacgaaggg
      481 tatgatgaag tgcgcagcac ttcttgcggt gaacagagga aacaagatgg tgtagaaaat
      541 ctgtttggga gaattctaga tcggaataat ctcaatcaag cgtatcttca agtagtcaga
      601 aataaagggg cggctggtgc agatgggatg accgtcgatc aactgcttcc ttacttgaaa
      661 gagcaccgcg aggaattact atccaattta cgccaaggga actataaacc tcagcctgtt
      721 ttgcgagtag aaataccgaa accaaacggc ggtgtaagaa aactaggaat tcccacggtt
      781 attgatcgta tgcttcaaca ggcaatcaat caagtgttac aaccgctatt tgaaccagaa
      841 ttctcagata atagttatgg gtttcgccca aatcgcagcg cacatgacgc agtgaagaaa
      901 gcaaaatctt attatgaaca aggttaccga tatgtggtgg acattgatat gaaagcctac
      961 tttgacaccg taaatcacga taagctgatg tatttcattg agcggaaagt aaaggacaaa
     1021 caagtcctaa aactgatccg tcaatactta atgagtggtg taatggaaaa tggactcatc
     1081 acaacaactg aagaaggaac gccccaagga ggcaatctct ctcccctgtt aagtaacatc
     1141 tacctgaacg aatttgatca agtgctagaa aatctaggac ataaatttgt tcgatacgcc
     1201 gatgactgca atatctatgt tagaagcaaa cgagcaggac agcgtgtgat gaagaaatcc
     1261 attcaatttc tcgaagaaca actaaagttg acggtaaatc gagaaaagag tgcggtagga
     1321 agtccattga aacgaaagtt tcttggattc tgcattctgc caacaaagaa aggtattaaa
     1381 attcgccctc acgcaaaagc aaaacagcgt gtgaaaagca agctcaaaca actcactaaa
     1441 agaaaccgtg gaagaagtat ccaacttatt ttaaaagaaa tcaagcaact aatgactggt
     1501 tggatcaact actatggaat tggtgagatg aaagaattca tgagggagtt aaacggttgg
     1561 ttgaagcgaa gaatcagaca atatatttgg aaacaatgga agaatccaag aaccaaaaga
     1621 aaaaatctga ttcgtttag
//
LOCUS       DAZUJO010000189         1614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000189 DAZUJO010000000
VERSION     DAZUJO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.198"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            535..>1614
                     /locus_tag="ACG8OL_002985"
     CDS             join(535..811,811..>1614)
                     /locus_tag="ACG8OL_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4818874.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          584 a          268 c          293 g          469 t
ORIGIN      
        1 gtcaagattt acctgaagat catccttttt aggaaattga agctttttat gttgcacaat
       61 gcatttataa tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga
      121 aacagataca cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg
      181 ttaatcttcc gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta
      241 aggttggcaa aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt
      301 tggttgacac ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt
      361 acctaaaaag tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat
      421 ttcaacttat atgttttagt gaactacccc cacttagctt ggataggcta caattaacta
      481 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
      541 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      601 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      661 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      721 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      781 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      841 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      901 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      961 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
     1021 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
     1081 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
     1141 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
     1201 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
     1261 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
     1321 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
     1381 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
     1441 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1501 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1561 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acac
//
LOCUS       DAZUJO010000190         1589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000190 DAZUJO010000000
VERSION     DAZUJO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.199"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..262)
                     /locus_tag="ACG8OL_002986"
     CDS             complement(<1..262)
                     /locus_tag="ACG8OL_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818875.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLS"
     gene            complement(264..>1589)
                     /locus_tag="ACG8OL_002987"
     CDS             complement(264..>1589)
                     /locus_tag="ACG8OL_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS4818876.1"
                     /translation="FNVTNIPMKIAFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMIS
                     FILTIIINRENFLIPSIYNFGYLLITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIF
                     LAALISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSL
                     ISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKS
                     IREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPL
                     PEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDL
                     DTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAF
                     LGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          626 a          262 c          188 g          513 t
ORIGIN      
        1 cggataaatc atccgagaaa aattgattat ggttagttaa caagttaatt ttacgtttaa
       61 aggaagaacc gattttccgg tgattcacaa aaacaacatc attaaatagc cctaattctt
      121 ttatacgctc actatatctt ctagcgtttt caaattcaga ataaataaat aaatctcctt
      181 ttgattctcg gtaaaaattt tgataaacat ttaatatatt aatcaaatgt aaaggagtcc
      241 aagcaatgaa agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt
      301 cccaaataat accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc
      361 tccaccattt catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca
      421 taattcactt tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa
      481 gcatagatac atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta
      541 tgcatgtttc catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt
      601 ttcaaaaatt ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa
      661 gttggtctta taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg
      721 gagttatcac tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat
      781 tcatttgact ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa
      841 tccttagcat aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc
      901 atccatttga tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca
      961 aacaacaaaa gaaataatac taaaaaagaa caaattacta cctgggcacc tctagacgaa
     1021 gaaagtgtaa tataaaatat ttgtacgata aaattcatca tataaaaaac acgaatcgtt
     1081 tttgttattt ttgaaattaa tacactaatt aaacttaaaa taatagaagt atatccaaac
     1141 atagctccaa catttggact tgtatagaca ccaaataacc gatgttcaat aaaaccttgt
     1201 cttgcaagta atcctggcgt tcctgttggg acatgataag aaatcaaaaa aataaattga
     1261 tagatagata ttagcgcagc taaaaaaatt atgataatga aaacattatt aaaatacacc
     1321 atttttttct ttttttcctc ttcactttgt gaaaagtctg caggaaaaat tactatcaaa
     1381 gtaatcagca aatatccaaa attataaata gatggaatta aaaaattttc tctgttaatg
     1441 attatagtta agataaaact aatcattgct ccaatcagta gccaagaaaa acgcatttta
     1501 aaaaaatttt tctttataaa aacctcatac aaaactataa ttccacccca agcaaaagct
     1561 attttcatcg ggatattagt tacattaaa
//
LOCUS       DAZUJO010000191         1574 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000191 DAZUJO010000000
VERSION     DAZUJO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.200"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1527
                     /locus_tag="ACG8OL_002988"
     CDS             61..1527
                     /locus_tag="ACG8OL_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS4818877.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          437 a          291 c          331 g          515 t
ORIGIN      
        1 aaatttttcg gtaaagatgt ataatatttt ctgaaaattt aaagaattgg ggtatagatt
       61 atggaaaaac agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc
      121 ttcactgaga tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat
      181 atgtacgata cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc
      241 atgtcgatct acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac
      301 cgagtgttag gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac
      361 atcgtcttgg ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt
      421 atcggaaccg cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag
      481 actgacttgc gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca
      541 ttattagcac cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc
      601 tctttagctg ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag
      661 acattagacg gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga
      721 ttcatccgta acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa
      781 ctaactggtc atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc
      841 ttaccgttgt attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa
      901 ccaagagtat gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa
      961 gaacaaggtt catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt
     1021 ggtgtaacga tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg
     1081 acaccagtat tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg
     1141 aaattctctc ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt
     1201 atgctttatg gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc
     1261 gtggttattg ctgaattgtg tctttcacca gttggtttgt ctgtgacaac aaaattagca
     1321 ccacgagcat ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa
     1381 gcaatcaatg cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt
     1441 attatcggtg cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc
     1501 aaaaaactaa tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg
     1561 cagcagttcc tgtt
//
LOCUS       DAZUJO010000192         1515 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000192 DAZUJO010000000
VERSION     DAZUJO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.201"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..1202)
                     /locus_tag="ACG8OL_002989"
     CDS             complement(24..1202)
                     /locus_tag="ACG8OL_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFS4818878.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT          446 a          274 c          283 g          512 t
ORIGIN      
        1 tcgtccttct gcaaaataat gggttactca gtaaacattg aagctaatgt atccgtcact
       61 tgttggaagc ctttatggct tctatttaag aatttttgat tgtatgtatc aaagatggat
      121 actaggaaac gctctagtga ttcttcattt tgaaactgct cttttctgcg gctgtatttc
      181 ttgatttgtt tattgaaaga ttcgattaaa ttagttgagt aaatgcttcg gcggatacca
      241 ggcggaaact cataaaaagt caataaatct tgatttttta tcagtgactg cgtcacttta
      301 ggataagttt tctgccactt ctcaatcata ctccctaaaa aggtatttgc ctcttccttt
      361 gagctagctt gataaacagc cttaaaatca tcacagattt cttttcgatc tttgacacgt
      421 actttatgag caatattacg ggaaacgtga acacagcaat gttggtattt agctttagga
      481 taaatctggt gaatcgtatc tttcatgcct tttaagccat cggtgataaa cagtaagaca
      541 tcatgaaccc ctctggagta aatgtcctgt aacaactcat tccaaacgta ggtcgattca
      601 gtcggagcaa tcgcataact tagtacttct ttagtgccgt cttctcgtat accaatggca
      661 atatatattg cttctttgga gacggtttgc cgttttaaag gaatataagt agcatccatg
      721 aaaatagcga catacttatc atttagagtt ctaactttaa atgcatttac ttcttcagtc
      781 agcactttag tcatgttgga catggtttgg ggcgtgtagt gatgcccata cattttctca
      841 atcaagtctg cgatttccga catggtaata ccttttcgga agaggtgaat gacggtctcc
      901 tctaaggtat cattcgtccg tctataagca ggaacagtct gttgcttgaa ctcgccgttg
      961 cggtcgcgcg gaatctggag atgaagttcc ccgtactcgg tcttgaccgt acggtcatag
     1021 gagccgttac gagaattacc cgtgttaaaa ccaatgcgat cgtacttttc gtaatcgagg
     1081 aaagccgtta actccgtttt gagaagcgta ttgacagctt tttccaaatg gacacggaat
     1141 aattcattca aatcgccttt gttcgctaga gtctttagaa tttctgtagt aaaatcgttc
     1201 atatggaagt cctcttttct gtgaatgtgt tgtggtaact ctattctaca gaagggactt
     1261 ccttttttct atttacacaa aatattttac actctcggta gaaaagaaat ttataaaagg
     1321 acttataaag attgattata taatatatga aaagctatta taaaagacat tagtattaaa
     1381 tagtttaaaa aaatgaaaaa taataaagga agtgagtcaa gtccagactc ctgtgtaaaa
     1441 tgctatacaa tgtttttacc atttctactt atcaaaattg atgtattttc ttgaagaata
     1501 aatccattca tcatg
//
LOCUS       DAZUJO010000193         1476 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000193 DAZUJO010000000
VERSION     DAZUJO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.202"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..1039
                     /gene="scrK"
                     /locus_tag="ACG8OL_002990"
     CDS             167..1039
                     /gene="scrK"
                     /locus_tag="ACG8OL_002990"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS4818879.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(1273..>1476)
                     /locus_tag="ACG8OL_002991"
     CDS             complement(1273..>1476)
                     /locus_tag="ACG8OL_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4818880.1"
                     /translation="PDNSACEGFFGRLKNEMFYQRDWKNTTINQFINKLDNYIHWYNN
                     QRIKLSLGGLSPLQYRKKQRYIC"
BASE COUNT          458 a          260 c          317 g          441 t
ORIGIN      
        1 atatcaattg tatgaaaaat tattagaatt ttaaaaaatg ataaaaattc aaaaagaaaa
       61 aatagtagtt atttacttgt catgttacta aataaaaaaa taatttaaat atgatatata
      121 gtcatttatt tagtgatttc attctaaaat aaagggagaa atgtctatgc tatacggtgc
      181 aattgaagca ggcggcacaa aatttgtgtg tgccattggc gatgaagaaa tgacgatcaa
      241 ggaaagagtg agctttccga caacgacacc tgaggaaaca atgcctttag tcattgactt
      301 cttcaaacaa taccaagcag atttagcagg aattgggatt ggctcatttg ggcctatcga
      361 tatccatcgc gattccgcca cttatgggta cattacctcg accccgaaat tggcatggca
      421 aaattttgat ttcatcggta ccatgaaaaa agaatttccg atcccgattt cttggacaac
      481 agatgtcaat gcggctgcct atggtgagta tgtcttcggc agtggcaaag gattgagcag
      541 tgtcgtttac tatacgatcg gtacgggtgt tggcggcggt gccttacaag aagggcgctt
      601 tatcgaaggg tttagtcatc cagaaatggg gcatatgctc gtcgtccctc atccaaaaga
      661 tgactttgct ggcagctgtc catttcatgg caactgttta gaagggatgg ccgctggacc
      721 cgccatcgaa aaacgcttag ggaaaaaagg acaagaggtt gcagaagatg atccttattg
      781 ggaaatcgaa gcctcctata ttgctcaatg tgcctacaat acaaccttga tgttttctcc
      841 agatgtgatc atttttggcg gtggtgtgat gaaacaacgc catatgctgc aaaacgtcca
      901 cgacgcattt gcaaaattgg tcaatgggta tgtcgaaaca cctgagttaa gcaaatatat
      961 cgtgacccca gcattagaag acaacgctgg aacgctcggt tgtttggcat tggctcgaga
     1021 agcggtctta cattcataaa cgaaaaaagg ctcttcgaca tttagtgttg gtagagaaag
     1081 atacaactga aaatttagtc ataaccagtt aggcagagtg cttaactggt ttttgtttta
     1141 cttggaagat caagccttga atgagataaa ttcttgctcg aaagttatgg aaatttcgat
     1201 agccgtaggc tacttgtatt gtgcaatagt tttttacaaa aaaaaggggt gcggatattt
     1261 ttttggacct ttttaacaaa tatatctttg cttcttccga tattgaagag ggcttagccc
     1321 tcctaaagat aacttaattc gttgattatt ataccaatga atataattat ccaatttgtt
     1381 tataaactga ttgattgttg tattcttcca gtcacgttga taaaacatct cgttttttaa
     1441 tcgtccaaag aaaccttcgc acgctgaatt atctgg
//
LOCUS       DAZUJO010000194         1419 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000194 DAZUJO010000000
VERSION     DAZUJO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.203"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..907
                     /locus_tag="ACG8OL_002992"
     CDS             <1..907
                     /locus_tag="ACG8OL_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFS4818881.1"
                     /translation="IIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNI
                     DSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIK
                     EQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELE
                     EKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSS
                     ASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLEN
                     YNGTVTFTKLAKAEQYRRSLELSKD"
     gene            979..>1419
                     /locus_tag="ACG8OL_002993"
     CDS             979..>1419
                     /locus_tag="ACG8OL_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS4818882.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANR"
BASE COUNT          615 a          167 c          222 g          415 t
ORIGIN      
        1 tattattgac gatgctgttt ctgtccctct tattttaaat aaacgagaga aaaataattt
       61 aaagaaaaaa atgctcattc catttgattc agaagtgaaa ggattactca ataaactaac
      121 tatttctaat atagactcga ctagggaaaa aatcataaaa atatttgata aaattaataa
      181 tttattcaag aatgacaatg ctgaattaag gaagggtctt ttagagttta aacttcaaga
      241 actagaactt cattactctt atttagtgaa aattaccgaa gaaaaagaac aacaaaaagc
      301 aataaaagaa cagatgatag aagaggaaaa agttcgtaga gaaattgatc gtgaaaagaa
      361 acgtattgat aaagaacaac gtcaattcaa ttctgaaatt tccaaattaa tcgcatatat
      421 gcaaaaagct aatgcagatg tggaaaaaga attatatgca aataaaattc aagaactgga
      481 agaaaaatta aaagaattag aagttatacg tgaaaatgta ttacaaagag aattaaatac
      541 tagagccggt tatgtgtatg taattagtaa tattgggtct tttggtgaag acatatataa
      601 aattggtatg actagaagat tagaacctat ggatagaata aaagaattga gtagtgcatc
      661 ggttccattt gaatttgacg ttcatgcgat gattttttca gaggacgctc cgtctttgga
      721 aactaaattg cataatcatt ttagaaaaca agaagttaat aaaattaatc aaagaaaaga
      781 atttttcaaa gtttcactag acgaaataga gaaagttgtt ttagaaaatt ataatggaac
      841 agtaacattt acaaaattag ctaaagctga acaatataga cgatctttag aattgtcaaa
      901 agattaaata tataaaaagc cttcgggctt tttcttttaa caattaatag gaacgtatat
      961 tcgaaaggaa tagaaataat gtggttagaa aaaataaatg acaaaaaatt taagtacacc
     1021 gaacgttata cggatccctt aactgaaaaa aagagaaaag tgtccgttac tttatctagc
     1081 aattcacgcc aagcttggaa tcaagctaac ttacttttga atgaaaaaat cgcagaaaaa
     1141 ataaaacgaa atgaagaatt accactaact tttggagaat taaaaactaa atgggatgaa
     1201 aaatacaagc caactgtaaa agaaagttcc tatcgaacaa ctcaggttta tctatcatta
     1261 atttcaaaat atataaaaga tgatgtactt gtaaaaaacg ttaatagtaa tcttatacaa
     1321 gatatgctag attactacta ttttgaaaaa aatttatcat ataattatgt aatacattta
     1381 aaaactttta caggaatgat ttttaaattc gccaaccgt
//
LOCUS       DAZUJO010000195         1359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000195 DAZUJO010000000
VERSION     DAZUJO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.204"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..>1359
                     /locus_tag="ACG8OL_002994"
     CDS             94..>1359
                     /locus_tag="ACG8OL_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS4818883.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMI"
BASE COUNT          428 a          269 c          257 g          405 t
ORIGIN      
        1 tcaccaacat cttttttgta taaaaaaata tagaaaagac accctgaaat cctttagaat
       61 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
      121 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      181 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      241 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      301 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      361 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      421 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      481 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      541 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      601 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      661 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      721 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      781 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      841 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      901 cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa
      961 ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt
     1021 aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta
     1081 cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca
     1141 ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact
     1201 ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca
     1261 aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc
     1321 tttcgcaact tttacaattt caaactacga attatgatt
//
LOCUS       DAZUJO010000196         1337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000196 DAZUJO010000000
VERSION     DAZUJO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.206"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1337
                     /locus_tag="ACG8OL_002995"
     rRNA            <1..1337
                     /locus_tag="ACG8OL_002995"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DAZUJO010000197         1308 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000197 DAZUJO010000000
VERSION     DAZUJO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.207"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..1098
                     /locus_tag="ACG8OL_002996"
     CDS             91..1098
                     /locus_tag="ACG8OL_002996"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS4818884.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1110..>1308
                     /locus_tag="ACG8OL_002997"
     CDS             1110..>1308
                     /locus_tag="ACG8OL_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFS4818885.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDS"
BASE COUNT          516 a          163 c          246 g          383 t
ORIGIN      
        1 acatcattat tgataattag tttagttata tattttttaa agattaagaa tcgaattata
       61 gatgactata attcatagga ggagatataa gtgtttaaag gaaaaacaat attagtaact
      121 ggcggaactg gttccattgg aagtgaaatt gttcgtcagc ttttaaaata tgaaccaaaa
      181 gctatcagaa tttatagtag aggtgaagaa aaacaatttt atatgcaaca agagctctca
      241 aatcacacta atttgagatt tttaattggt gatatcagag actctcaaag gattgattat
      301 gcatgtcgtg atgtggatat tatttttcat gctgccgcaa tgaaacacgt tccagcaagt
      361 gaatataatc ctatggaagc tgtaaaaaca aatgtgatgg gaactcaaaa tgtaatagat
      421 gcagcgatca aaaataatgt aaaacatttt gtaggtatca gtacagataa agtagtgagt
      481 cctactaaca ccatgggtgc gacaaagtta ttgagtgaaa aattaataat agctgccaat
      541 cagtataaag gaaaaacaga aacaatattt tcttgtgtaa ggtttggaaa tgtcatgggg
      601 tcaagtggat ctgttattcc tctttttagt aaacaaataa aaaacaataa agatattact
      661 ttgacagatg ttgatatgac acgatttatg atgtcaatac cggatgctgc aagattagtt
      721 ctgtctgctg ctatgaattc aaaatttgga gaaacatatg tcttaaaaat gccggcaatc
      781 aatattttaa ctttagctca atcattaata aaaatatata atgatacaca tgatgaaaaa
      841 tatagaggtg aaataaaagt tgtaggtatc aggccaggag aaaaattata tgaagagcta
      901 atgactacag acgaaattga acgctcatat gaaaatgata aaatgtatat cattccacca
      961 cactatcatg ctactgacga actgtacaaa gattatgaaa aaattaaaat cactgcatac
     1021 tcttcagatg caacagaatt aatgagtgga caggaattta ttagaatcgc aaaagagaat
     1081 aatgtcgtga cttggtaagg agttttatga tgaaatcaga agtatatgta attgcagaaa
     1141 ttggtgtaaa ccataatgga agtctagatt tagctttaga aagtattgat aaagctaaag
     1201 aatgtggagc taacgcggtt aaatttcaaa catttaaaac gaataagcta gtttccaaaa
     1261 aggctccaat ggcaaaatat cagaaggata atcttaaaga ggacagtt
//
LOCUS       DAZUJO010000198         1301 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000198 DAZUJO010000000
VERSION     DAZUJO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.208"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..339)
                     /locus_tag="ACG8OL_002998"
     CDS             complement(40..339)
                     /locus_tag="ACG8OL_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS4818886.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(345..575)
                     /locus_tag="ACG8OL_002999"
     CDS             complement(345..575)
                     /locus_tag="ACG8OL_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818887.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(834..947)
                     /locus_tag="ACG8OL_003000"
                     /pseudo
     CDS             complement(834..947)
                     /locus_tag="ACG8OL_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          449 a          216 c          196 g          440 t
ORIGIN      
        1 ataaaatccg ttccgggcta cctttttagg tagcctactt taatctttgt atctttctgg
       61 atcaacgaaa gtatacttta tatagtcata acgccgatga tcgcttcgag cgtctagcac
      121 atcagtcacg atatcaaaca aaaaatatac gtctttcttc attctagttt tcgcagcagg
      181 gattttaaag tagttcttat tagaatagta gagattaatt aataagctat cttcgattgc
      241 taaaaagaaa acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg
      301 atcaaattta aaccataatt gcgtctcatt aaaaagcata accattactc cgatctattt
      361 ttaaacttag tctctacctc taatatatat cgagttttta ttttgccttc agagaatact
      421 gtttcttttt ttgcagttac aggttgttta ttttcggaaa aagctaatat agctaaaatt
      481 gaaacatcca tctggaattt gtctttttcg ttgctttgct cataaaaatc tgcatattca
      541 tcactaatat tttttctaat aaattcttcc atcataaaga tcacctcgaa aagagtatac
      601 gaacaaacgt tctttttgta aaggtggaat ttattatact aaataaatag gtgaacaagt
      661 acttgtgcca aattatgtgc caaaaaaaat cgaatttaat aaaacacaaa caaaaagaaa
      721 tcctattatg ataacatttc ttataataac aaacacaaca gaagacgtgt aatagttagt
      781 caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt tgtataagta
      841 gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact
      901 ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga
      961 gaaaataggg aaaaattgat tactaaagaa aaacttacta ggtcgacggt gtagttgatt
     1021 ttcatacttc aatagattcg aattaaacga tttcgattgg cttataccag tcctatccaa
     1081 cgaaagatat atcgttctta actctagcgg tataacttga agctttacgt aaagccaatg
     1141 ggaatgaact ggagatgact ttttattatt tttagtaata gctgatgggt taatacttac
     1201 aactttcctt ttcaaagtac catataaaaa aacggtgggg ttgtttagcg aaataaagga
     1261 tctaagccaa tagcttagat cctttattct atgctttata t
//
LOCUS       DAZUJO010000199         1262 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000199 DAZUJO010000000
VERSION     DAZUJO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.210"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..744
                     /locus_tag="ACG8OL_003001"
     CDS             <1..744
                     /locus_tag="ACG8OL_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFS4818888.1"
                     /translation="YCIQNKIDFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLR
                     KIDMYGLPVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLK
                     EMFGIPVGYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFV
                     KHIRNTEIFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGI
                     APKFLYQYIGKKVKRPISADSVLLEEDVE"
     gene            741..>1262
                     /locus_tag="ACG8OL_003002"
     CDS             741..>1262
                     /locus_tag="ACG8OL_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS4818889.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAP"
BASE COUNT          418 a          167 c          271 g          406 t
ORIGIN      
        1 tattgtatac aaaataaaat tgattttttg tctactcctt ttgatgaaga aagtgcagac
       61 tttctaaaac aaattggtgt acaaggattt aaaattggct caggagattt aacaaattta
      121 ccttttctta gaaaaataga tatgtatggg ttgccagttt tattatctac aggtatggct
      181 gatattcatg aggtcagtga ggctgtagat tgtttcatag atagtccagt tactatttta
      241 cattgtactt caaattatcc ggctagttct gaagatatta atttattggc gattaacaca
      301 ttaaaagaaa tgttcgggat acccgttggg tactccgatc attcattggg atatgatgtt
      361 ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga aacattttac cttggataag
      421 gaactacctg gtccagatca taaagcttct ttagatcctc aggaatttgc agattttgta
      481 aagcatatta gaaacactga aatattttta ggtgatggtg ttaagcgagc aatgccttct
      541 gaacttagta caaaagaagt atcaagaaaa agcctagtgg tcactgagaa tttaaatgtg
      601 ggagatattc ttacagagaa atctcttaca ataaaacgtc ctggaaatgg aatagctcct
      661 aagtttttat atcaatatat tggcaaaaaa gttaaaagac caataagtgc agatagtgtt
      721 ctgcttgagg aagatgttga atgaaaaaaa tacttatagt aagtggaaca cgagctgatt
      781 atggaatata taaacctgtt gctgagaatt tactacaaga taattgggat gtatcatttg
      841 ttgtttctgg aatgcattta tcccataaat atggatttac taaagaattg atcttagctg
      901 acggatttag aattagtgga gaagttcaaa gtttgttttt agagaacaat aagggaaaca
      961 tggctcggtc tgtggcatta gagataaatg gtttcacaaa tatatttgaa caaactagcc
     1021 ctgattttgt attattacta ggggatagag gagaaatgtt ggcagctgcg atagcttgtt
     1081 tgtatcttgg aatcaaaaca gcacatattc acggaggcga agtgtctggt actgtagatg
     1141 aatcaataag acatgccata acgaaaatag catctgttca ttttgctgct acggaaaaaa
     1201 gcaaagagcg tttaataaaa atgggggaag attcttggag agtttttccc gtcggagctc
     1261 ct
//
LOCUS       DAZUJO010000200         1251 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000200 DAZUJO010000000
VERSION     DAZUJO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.211"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG8OL_003003"
     CDS             <1..963
                     /locus_tag="ACG8OL_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS4818890.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ACG8OL_003004"
     tRNA            1152..1225
                     /locus_tag="ACG8OL_003004"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
BASE COUNT          412 a          239 c          256 g          344 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct g
//
LOCUS       DAZUJO010000201         1203 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000201 DAZUJO010000000
VERSION     DAZUJO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.212"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1153
                     /locus_tag="ACG8OL_003005"
     CDS             <1..1153
                     /locus_tag="ACG8OL_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS4818891.1"
                     /translation="REEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLI
                     SNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDP
                     LGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAG
                     DVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQ
                     AVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQ
                     YACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVY
                     KTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          389 a          217 c          258 g          339 t
ORIGIN      
        1 acgagaagaa attcgtttcc atcaggctat gttggattgt ctcacccatt atcaacatac
       61 ctattgttgt aaaagctgcg aaaaagaagg actttcttct tttaaaaaag cgatcgttcc
      121 taaacctttg atttctaata gcttaggttc caatagttta gtggcggaaa cgatccggat
      181 gaaatttggt cagaaagttc ctgcctatcg gcaagagaac tattggaaac aaacacatgg
      241 gttggatatt tcgagagata atatcaccaa ttggcatatt aaagcagtgc aaaacgcgtt
      301 agacccttta ggagaacgat taagagtcta tcttaatcaa gaagagattc tacacgggga
      361 tgaaacaagt tatcgtgtga ttgaaagtgc caaaacagat acctattact ggcagttttg
      421 tacaggaaaa gacagtcagc atcccatcgt ttattatcac cacgacgaaa gtcgtgctgg
      481 agatgttccg aaaacttttc tcaaggagtt tacagggtat ttacactgtg acggctatag
      541 cgggtataat gccgtggaaa gtgtacgact tgtctattgt ttcgcgcatg ttcggcggaa
      601 atttttcgaa gcaattccaa aaggaaagaa aaacacggat attcccgcag ctcaagcagt
      661 caaacaattg gataaatggt ttgtcctaga aaagaaatgg aaagactttt ctcctgagaa
      721 gcgattaagc tgtcggcaac aagaactgcg tccattattt attgcttttt atgagtggat
      781 ggcaacgata gatcctgttg caaaatccaa gttggatgca gccgttcaat atgcgtgtaa
      841 actacgaagt ggttttgaac ctatttttga agatggtcgt ttagaactca caaataatcg
      901 tgcggagcga aatatcaaag agctggtgat aggacggaaa aattggttgc attccacaag
      961 tcttgaagga gcacgaactt caggaatcat tctgagtgta tataaaacag cagaacttaa
     1021 cggattgaat cctgtaaagt atctagagtt tctatttgat aagataccaa atcttcccgt
     1081 actttcagca gaaactttag atcaactgtt gccttggaac aaggatgtac aacaacattt
     1141 ttctcgtaac taaaaacgcc aaacattccc gattctattc tataggtcgg gttatttgac
     1201 gta
//
LOCUS       DAZUJO010000202         1195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000202 DAZUJO010000000
VERSION     DAZUJO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.213"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8OL_003006"
     CDS             <1..1076
                     /locus_tag="ACG8OL_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4818892.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZUJO010000203         1148 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000203 DAZUJO010000000
VERSION     DAZUJO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.214"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1061)
                     /locus_tag="ACG8OL_003007"
     CDS             complement(<1..1061)
                     /locus_tag="ACG8OL_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818893.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          303 a          255 c          182 g          408 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaactttt
     1141 tatacttt
//
LOCUS       DAZUJO010000204         1147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000204 DAZUJO010000000
VERSION     DAZUJO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.215"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..945
                     /locus_tag="ACG8OL_003008"
     CDS             <1..945
                     /locus_tag="ACG8OL_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS4818894.1"
                     /translation="INDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNVAANMKNNY
                     NGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRA
                     YIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKE
                     KLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHV
                     AGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNF
                     PSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          389 a          203 c          231 g          324 t
ORIGIN      
        1 attaatgacg agtataattt agcgccgaat caaggagact ccagattagc aattcctaac
       61 aaaattattt tgcacgaaac tggaatagat gcaccagcaa gaaacgtagc cgccaacatg
      121 aaaaataatt ataacggaag caattcttat actacagatg ttattggtga cggtgggatt
      181 gtttaccgtg tgggtgagca aggatatgtt tcgtggggag ctggtaacgc taatccttat
      241 gcgcctgtac agattgaatt acagcgcaca tatgataaag cattgtttga aaaaaactat
      301 cgagcttata ttgaatatac aagagatagt gcaaaaaaat atggaattcc attgactctt
      361 gatcaaggaa cttctttatt tacaaaagga atcatttctc atttgtgggt gacaaattat
      421 gtttggggga accacacaga tccatatggt tacttatcgc aaatgggagt tagcaaagaa
      481 aagcttgctt atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac
      541 aagcctgtca ttgatccaac acgagctggt gcagctaatc ctacgctgac agatggaaca
      601 aatcacgccc acattgatca gtttggggaa atcgaaaacg caaacttgca tgtggctgga
      661 tggcacattg ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa
      721 gaattagctc gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat
      781 acttctggaa atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa
      841 gtctatgtaa tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa
      901 gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt
      961 gaggagcagt acataactat attgacaact ataaaaatta ttcgataaaa tagtgatgtt
     1021 atcgcatatc ttcactatca cccataaata gtcacactcc aagctatgcg ataacaggtt
     1081 tgttgccaca cattctactg gttgattgtt tatggcttta tgtggcaaca accagtaccc
     1141 ttagctc
//
LOCUS       DAZUJO010000205         1110 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000205 DAZUJO010000000
VERSION     DAZUJO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.216"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..>1110
                     /locus_tag="ACG8OL_003009"
     CDS             120..>1110
                     /locus_tag="ACG8OL_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818895.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQN"
BASE COUNT          380 a          139 c          146 g          445 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
     1021 taggaatctt atctaaaatc acttatataa tgggtataat atctattcta ctttgtacaa
     1081 aaatatttcc atggtttgcc ttacaaaata
//
LOCUS       DAZUJO010000206         1075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000206 DAZUJO010000000
VERSION     DAZUJO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.217"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1075
                     /locus_tag="ACG8OL_003010"
     CDS             <1..>1075
                     /locus_tag="ACG8OL_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFS4818896.1"
                     /translation="KQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIER
                     DFQIEFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIG
                     DIYLTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDA
                     IDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNN
                     VSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSF
                     KLKKILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFF
                     SIEDMILGMNISKFSNQLNRHFND"
BASE COUNT          398 a          136 c          181 g          360 t
ORIGIN      
        1 taagcagcag ctttttgaga aatatgatag atattttaga caaaagaatg ttgatatcca
       61 aaataattca gctttaacat atcccaccta tcaagagatt cgatttagac agtatgttaa
      121 ggaaatagag agagattttc aaatagaatt tgactattat tatcttcaaa taacgtctat
      181 ctttttgaat tcttggattt atcaaatcca attaacatcg gacgataaat tatattcctt
      241 catcaatgat attaatagtt ggtgtagtcg agaagttcaa ttagtcaatc gttttattca
      301 aataggggat atttatttaa caaaaattca aaaaaatgtt ttggttattt tatttaagtt
      361 aatgtgtagt aaagaatttc agcaaaaacg tttagcactg attttggctc atggagatct
      421 aacggcaacc tgtatagcaa gtactgctaa taggatcgtt gagagatata tatacgacgc
      481 cattgatatg ccactaatga cttcaagtag tgagattgca gacaaaatta atagatactt
      541 acaagagtgt accggatatg aagaaattat tttacttgtt gatatgggtt cactggagaa
      601 aatttatcta aatttggaac tagacgctca aaaaaaattc ggactactaa acaatgtttc
      661 aacaagaatg gctattgatg ttgcaaatca gatattatta gatttaccgc ttaaacagat
      721 tcttgaacaa gtaaaagaaa atcaaaatgt ggattatatt tatgaatcat caaatattaa
      781 gaaggatatg attgtctgtt cttgtgcaag cggattagga acttcgttta aattaaaaaa
      841 gatacttgaa gatagctttc caagggatac tgatcttgtc atagaaacgt gtgactatta
      901 ctctttagtg aatgaaaact atttatcgga gcttcgtaaa gagaacaata ttctttttat
      961 tgttggaaca ctaaatccac atctagaaga tgtagaattc ttttcaattg aagatatgat
     1021 tttaggaatg aatataagta aattctccaa tcaattgaat agacacttta atgat
//
LOCUS       DAZUJO010000207         1068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000207 DAZUJO010000000
VERSION     DAZUJO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.218"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..373)
                     /locus_tag="ACG8OL_003011"
     CDS             complement(<1..373)
                     /locus_tag="ACG8OL_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818897.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQM"
     gene            complement(475..828)
                     /gene="tnpB"
                     /locus_tag="ACG8OL_003012"
     CDS             complement(475..828)
                     /gene="tnpB"
                     /locus_tag="ACG8OL_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS4818898.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(818..1012)
                     /locus_tag="ACG8OL_003013"
     CDS             complement(818..1012)
                     /locus_tag="ACG8OL_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818899.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          302 a          213 c          188 g          365 t
ORIGIN      
        1 tcatctgtga accgcaatgt tcacaaaatt gttcttccaa aggaaggaga tgttctttct
       61 ctgttatagg taagtgtttg attttgtctg ctttcgttcc tacaactcgt tgatgtcctt
      121 ttactggaat aatggtggca gctgcttctg gaacacttac agaaatttct tcatcactaa
      181 aaagattcaa ttgcccattc gtttcctcaa agagagtctc tttagagcgt ccaaataatt
      241 tgttggttaa ataattgacc ttttcattca atagttggat ttcagttgta agattctgaa
      301 tggtttgttg ttgtgcttga ttggttttat gggcttcttg aagcaactga agcaacgtat
      361 ctgtagtttc caaaattttt caccaccttg aatcgaactg attttgttac agaataccac
      421 aagaaacgca caagaaatag gggttcaaca cattttttgt gttaaccccc cagcttaaaa
      481 aactcctttt tttcctggca aaatagctcg aggttgatcg atacttaatc cttctaacaa
      541 ccatcgaacc tgttggttcg ttaaggttcg aacttcgtct ttcgtccgag gccattttaa
      601 cttgccgttt tctatccgct tataacagag aagaaaacca tcaccgtccc aatagagaag
      661 tttgaaacgg tcttgacggt tcccgcaaaa taaaaaaacg gcatcttcgt aaagctcgag
      721 ttgatactct tgttgaataa gactggcaag accatcaatt ccttttcgca aatctgtttt
      781 tccgcacaca atataaatat gtttggtttg aagtaaatca taaagcattg gacaacagtt
      841 ctttcaaaat agcttgaaga atataattgt taataccgtt atagatcaaa acttcagaac
      901 cattctctct tttgatgcgc atcgctaact tggcagccgc gggtttacct ttttctggtg
      961 cactcttttc gtttcctaat tggacaggaa caatctcatt taatttatac atagaaaacc
     1021 tccagacttc ttgatagctc aagtatatct tgaggtgcat ttgttagt
//
LOCUS       DAZUJO010000208         1056 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000208 DAZUJO010000000
VERSION     DAZUJO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.219"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1016
                     /locus_tag="ACG8OL_003014"
     CDS             <1..1016
                     /locus_tag="ACG8OL_003014"
                     /inference="COORDINATES: protein motif:HMM:NF016309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HFS4818900.1"
                     /translation="NFSLLADRYLEKILQGIIEEKKREDKFTSAYSQSVQNYNTNSYN
                     RKIENLKNNQQKIKHFYAYILEFLDTPVMKNISSRRPINVPKSLVMNPKYNTIYKMYL
                     KSRNNRNTLNFSNRYQYYWKSTDVLYEIWCFIKIIRSLSLQGYTPISGWIFENDILSQ
                     ELPFLHEDTTVVLSKNDIRINLVYNNSMEKEYFSNTLDIPVKTSSKRNKPDIRLDIIL
                     QDKYYIGSLIFEVKYKKLDNILDNDKGRQRQQLMAYKQNTMSSILAFPEILTRSLQAV
                     SAVFALYPSNEGRKKTAPKYYEKEGIFFHLLNPSSDEKELSVKIQTSIEERISLFNQQ
                     SRN"
BASE COUNT          432 a          154 c          139 g          331 t
ORIGIN      
        1 aaaatttttc acttctagct gatagatatt tagagaaaat cctacaaggt attattgaag
       61 agaaaaaaag agaagataag tttactagcg cgtattctca aagtgtacaa aactacaata
      121 caaatagcta taatagaaag attgaaaatt tgaaaaacaa tcagcaaaag ataaaacatt
      181 tttatgctta tatccttgaa tttttagaca caccagtaat gaaaaacatt tcatcaagaa
      241 gacctataaa tgttccaaaa tcacttgtta tgaaccctaa atataacact atatataaaa
      301 tgtatttaaa aagtagaaac aatcgtaata ctttaaattt ttcaaataga tatcagtatt
      361 attggaaaag tacagatgta ctctatgaaa tttggtgctt tataaaaatt attagatctc
      421 tatccctgca aggctacact ccaatttctg gatggatttt tgagaatgat attctttcac
      481 aagaattgcc gtttttacat gaagacacta ccgtagtatt gagtaaaaat gatatacgta
      541 ttaatcttgt atataataat tccatggaaa aagaatattt ctccaataca ctagatatcc
      601 cagtaaaaac ctcttcaaaa cgaaataaac ctgatattag attagacatt atccttcaag
      661 ataaatacta tatcggaagc ttaatatttg aagtaaaata taaaaaacta gacaatatac
      721 ttgataatga taaaggtagg caacgtcaac aattaatggc ttataaacaa aatactatgt
      781 caagtattct agcatttcct gaaattctta cacgttcttt gcaagctgtc tctgctgtct
      841 ttgctttata tccttccaat gaaggaagaa aaaaaactgc acctaaatat tatgaaaaag
      901 aaggtatttt ttttcactta ttaaatcctt catcagatga aaaagagctt tcagtaaaaa
      961 ttcaaacaag tattgaagaa agaatttcat tatttaatca acaaagtaga aattaaaaag
     1021 atgaagagcg tctctttata caagagagcg ctcttc
//
LOCUS       DAZUJO010000209         1045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000209 DAZUJO010000000
VERSION     DAZUJO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.220"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8OL_003015"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8OL_003015"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS4818901.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DAZUJO010000210         1001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000210 DAZUJO010000000
VERSION     DAZUJO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.221"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1001
                     /locus_tag="ACG8OL_003016"
                     /pseudo
     CDS             370..1001
                     /locus_tag="ACG8OL_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DAZUJO010000211          983 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000211 DAZUJO010000000
VERSION     DAZUJO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.222"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            555..>983
                     /locus_tag="ACG8OL_003017"
     CDS             555..>983
                     /locus_tag="ACG8OL_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818902.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQ"
BASE COUNT          345 a          185 c          164 g          289 t
ORIGIN      
        1 agacactcct cttaatatac agtcctattg tataaagtgt tactatatac atagtaactg
       61 tatacataaa aaaagtcaag tattagaaaa aagttcaaaa gatcaagtgt gcgcttcgct
      121 cccactcccc tcgacgtttt agtagccttt ccctcacttc gttcagtcca agccaactaa
      181 aaagttttcg ggatacattc tccttctctt cctaataatc aattaaaatt ttaatcttta
      241 tatttctgct aatcactcag gaaacagtac agaaaaacaa acacgtatca tagatataaa
      301 tgtaatggca tagtgcgggt tttattttca gcctgtatca tagctaaaca aatcgagttg
      361 tgtgtccgtt tttgggcgtt ctgctagctt gtttaaagtc tcttgaatga atgtatgttc
      421 taagtcaaaa aactctgaca gcgcctttat atagataaag tccgtataat tgtgtaaaag
      481 taaaaaggcc atataacagt ccttttacgg tacaatgttt ttaacgacaa aaacataccc
      541 aggaggactt ttacatgacc caagtacatt ttacactgaa aagcgaagag attcaaagca
      601 ttattgaata ttctgtaaag gatgacgttt ctaaaaatat tttaacaacg gtatttaatc
      661 aactaatgga aaatcaacga acagaatata ttcaagcaaa agaatatgaa cgaacagaaa
      721 accgacaaag tcaacgaaat ggctattatg agcgcagctt tacgacacgt gtaggcacgc
      781 tagaattaaa agtacccaga acacgtgatg gccatttttc acccacagtg tttgaacgtt
      841 atcaacgaaa cgaaaaagcc ctcatggctt caatgttgga aatgtatgta tcaggcgttt
      901 caactcgtaa agtatcaaaa attgtggaag aactttgtgg taaatccgtc tctaagtcct
      961 tcgtttctag cttaacagaa cag
//
LOCUS       DAZUJO010000212          978 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000212 DAZUJO010000000
VERSION     DAZUJO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.223"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>978
                     /locus_tag="ACG8OL_003018"
     CDS             82..>978
                     /locus_tag="ACG8OL_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS4818903.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYE"
BASE COUNT          333 a          182 c          192 g          271 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaa
//
LOCUS       DAZUJO010000213          948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000213 DAZUJO010000000
VERSION     DAZUJO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.224"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..>948
                     /locus_tag="ACG8OL_003019"
     CDS             124..>948
                     /locus_tag="ACG8OL_003019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818904.1"
                     /translation="MNKKEIKEFVKANRWFTYEQAENELRKHWNSDCDKDGDYLKIKR
                     RQIQKILRSDIIGTYLEMDKRDVGITKEEIFMKKLYGWTAEETFYLEYYDSERDDIIE
                     EEYTEKLHVFPKYDRLFPNNESSMILSTWDEQLGEDEYTAMKVFMREVYEKVFQLDTR
                     GNTAYIMNESYLFALKHELERREYPTKIVYLKPHSPKEITKNLSNTKHDYDILQNSDI
                     LLKDFNEQIENLVITHNKAKDVEIERYFNIDNFFIKWQNTYPEAINRIISSLKEKEL"
BASE COUNT          392 a           92 c          177 g          287 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gttattaaat
       61 tattagtcta cttgtgtatt atggtcttca taagaaaaat gaaattgaat aggagcagaa
      121 gtgatgaaca aaaaagagat aaaagagttt gttaaagcca ataggtggtt tacatacgaa
      181 caagccgaaa atgaattaag aaaacattgg aattctgact gtgataaaga tggtgattat
      241 ttaaagataa aaagaagaca aattcaaaaa atcttacgtt cagatattat agggacatat
      301 ttagaaatgg ataaaagaga tgttgggatt actaaagagg aaatatttat gaagaaatta
      361 tacgggtgga ctgcggaaga aaccttttac ttagagtatt atgattcaga aagagacgat
      421 attattgagg aagagtacac agagaagctt catgtttttc caaaatatga taggttgttt
      481 cctaataatg aaagtagtat gattctttct acgtgggatg aacagttagg ggaagatgaa
      541 tatactgcaa tgaaagtttt tatgagagaa gtatatgaga aagtatttca attagatact
      601 aggggaaata ctgcatatat tatgaatgaa tcgtatttat ttgcattgaa gcatgaatta
      661 gagagaagag agtatcctac taaaatagtg tatttaaaac ctcactcacc taaagaaata
      721 actaaaaatt tatcgaacac taaacatgat tatgatattt tacaaaattc agatattctt
      781 ttgaaggact tcaatgaaca aatagaaaat ttagtaatta cacataacaa agctaaagat
      841 gttgaaatag agcgatattt taatattgat aactttttta ttaaatggca aaatacttat
      901 ccagaagcaa taaatcgaat tattagtagt ttaaaggaaa aggaatta
//
LOCUS       DAZUJO010000214          943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000214 DAZUJO010000000
VERSION     DAZUJO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.225"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8OL_003020"
     rRNA            <1..>943
                     /locus_tag="ACG8OL_003020"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZUJO010000215          940 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000215 DAZUJO010000000
VERSION     DAZUJO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.226"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8OL_003021"
     CDS             <1..459
                     /locus_tag="ACG8OL_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818905.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8OL_003022"
     CDS             473..904
                     /locus_tag="ACG8OL_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818906.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          358 a          135 c          197 g          250 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga
//
LOCUS       DAZUJO010000216          938 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000216 DAZUJO010000000
VERSION     DAZUJO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.227"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      337..383
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            396..899
                     /locus_tag="ACG8OL_003023"
     CDS             396..899
                     /locus_tag="ACG8OL_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS4818907.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
BASE COUNT          262 a          176 c          185 g          315 t
ORIGIN      
        1 cctagcaagc gcatgatcca ttacttacgt aaggagaagt tccaaggttc ggcatgtcca
       61 cttagatctt cagctgcttg ctagaccgtt ctctagtaag aggttgctca aattcccaca
      121 tcctttttta tttatttaag tttcacaaca attctgttct tgcagatcct acttcgtctg
      181 ctttactggg aattcttatg accccttaca cttactttat accatatccc acgattttta
      241 gcaaacgttt acattgttta agcttttgct tttttaaaag cagataagac ggattgaaat
      301 aaatgaaaag tctgttataa ttattgtttg tttcacataa ccgcggttca tcaaccatcc
      361 cgcgttaaaa aaactaggag aaaagaggag agaaaatgca aaaaacgaaa agcagacaac
      421 ggttatccgt aatcgttgta aatggcatca tcatggctct ttatctagcg ttaactattt
      481 tagttgcgcc tgtatcctca gggccgatcc agtttcgtat ttcagaaagt ttgaatcatt
      541 tagttgtctt taaccgtaaa ttgctttggg gagttttagg cggggtcatc gtctataatg
      601 ccttctttgg ttttggtatc atggatgtgg tttttggtgg cggccaaaca ttgctttctt
      661 taggcctgac agctttattg caaaacaaag tgaaaaatgt aaagatgcgt ttagtattga
      721 acacgatctt ctttaccatc agcatgggac tgattgctta tatgttagtt ccaacaggtg
      781 gccaagcatt ttggacaacc tatggcacgc ttgcattaag tgagtttatc gtcatggctg
      841 tttcagcacc attgatgtat tatttagatc gcgcgcttga ttttgaaaag cgtgtctaat
      901 tataaaaagc gaaaaaagaa agcagattag ctgttttc
//
LOCUS       DAZUJO010000217          910 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000217 DAZUJO010000000
VERSION     DAZUJO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.228"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8OL_003024"
     CDS             <1..888
                     /locus_tag="ACG8OL_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS4818908.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          120 c          149 g          252 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc
//
LOCUS       DAZUJO010000218          892 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000218 DAZUJO010000000
VERSION     DAZUJO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.229"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>892
                     /locus_tag="ACG8OL_003025"
     CDS             <1..>892
                     /locus_tag="ACG8OL_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818909.1"
                     /translation="EEKIIQYDGLAKLKDAKVVSGEQRINREDLSDEFKNVVSLEATN
                     NTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSG
                     KIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILM
                     YSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLG
                     GMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNV
                     LLKVIVDGFINSEDSMISGGS"
BASE COUNT          366 a           77 c          154 g          295 t
ORIGIN      
        1 gaagaaaaaa taattcaata tgatggatta gcaaaattaa aagatgctaa agtagttagc
       61 ggagaacaaa gaataaatag agaagattta tctgacgaat ttaagaatgt tgtttcactc
      121 gaagctacaa ataatactaa aagaaatatt ttatttagta gtggagtatt tacaattaaa
      181 gaaggtaaaa atatagaaga aaatgataag aattcaatta ttgttcatga agaatttgct
      241 aaacaaaaca atctaaaatt aggtgatgaa gttgatcttg aattactaga tattgaaaaa
      301 agtggaaaaa taaaaagtca taaatttaaa attataggga tcttttctgg taaaaaacag
      361 gaaacatata caggattatc ttctgatttt agtgaaaata tggtgtttgt agattattca
      421 acaagccaag agatattaaa taattcagaa aataatgaaa ttgcaaataa aattttaatg
      481 tattctggta gtgcagaatc tacagactta gccttaaaca aattgaaaga gcttaaaatt
      541 gatgagtcaa agtattttgt tgaaaaagat tctaatgcgt ttgaagagtc tttagagtca
      601 gtgagtggaa taaaatatat aataaaagta atgacttatt ctattatgtt aggtgggatg
      661 gttgttcttt cattaatctt gattctatgg ttaagggaaa gaatttatga aataggtata
      721 ttcttatcta ttggaacatc taagatacaa attataatgc aatttatatt cgagttaata
      781 ttcatatcga taccaagtat aatatcttcc ttatttttag gaaatgtatt actaaaagta
      841 attgtagatg gattcattaa ctcagaggat tcaatgattt ccggtggaag tt
//
LOCUS       DAZUJO010000219          787 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000219 DAZUJO010000000
VERSION     DAZUJO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.230"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACG8OL_003026"
     CDS             <1..716
                     /locus_tag="ACG8OL_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS4818910.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttat
//
LOCUS       DAZUJO010000220          774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000220 DAZUJO010000000
VERSION     DAZUJO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.231"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..>774)
                     /locus_tag="ACG8OL_003027"
     CDS             complement(248..>774)
                     /locus_tag="ACG8OL_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818911.1"
                     /translation="LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          319 t
ORIGIN      
        1 gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat
       61 tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag
      121 atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg
      181 atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat
      241 ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta
      301 tatcttctaa attagaagat atttgtatat caatatggaa tatattacct tttttgtatt
      361 tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag
      421 ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt
      481 tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac
      541 ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca aaatctaaac
      601 tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg
      661 gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat
      721 agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc aaac
//
LOCUS       DAZUJO010000221          770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000221 DAZUJO010000000
VERSION     DAZUJO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.232"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..483
                     /gene="ssrA"
                     /locus_tag="ACG8OL_003028"
     tmRNA           117..483
                     /gene="ssrA"
                     /locus_tag="ACG8OL_003028"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(529..>770)
                     /locus_tag="ACG8OL_003029"
     CDS             complement(529..>770)
                     /locus_tag="ACG8OL_003029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818912.1"
                     /translation="KNLFEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEY
                     VLGISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT          249 a          126 c          151 g          244 t
ORIGIN      
        1 gaaaggggca gttattatat aaataatggg atatgattcg cttttttatt gaatttatcc
       61 caatatatgc tatactaatt ttgtatgaaa aatacacgta acccttgtta gctaacgggg
      121 acgttacgga ttcgacaggc acagtcgagc ttgaattgcg tttcgtaggt tacgtctacg
      181 taaaaacgtt acagttaaat ataactgcta aaaacgaaaa caactcttac gctttagctg
      241 cctaaaaaca gttagcgtag atcctctcgg catcgcccat gtgctcgagt aagggtccta
      301 actttagtgg gatacgtttc aactttccgt ctgtaagttg aaaaagagaa catcagacta
      361 gcgatacaga atgcctgtca ctcggcaagc tgtaaagtga atccttaaat gagttgacta
      421 tgaacgtaga tttttaagtg gcgatgtgtt tggacgcggg ttcgactccc gccgtctcca
      481 tatatgtatg gttgagacag ataaacatgt atgaaaaacg ttgttaaatc aacgtttttt
      541 tattttggtc aaggagagaa aagtagagaa atacaaaatt ttttgcattg attttgcatt
      601 caaaaaaaca ttcaaacatt tagaaatccc caatacgtat tcaggaaaat ctattttaag
      661 gtttacaaaa agaacttttg ttcgtacact ctttttgagg tgaactttta tgctgatgga
      721 aggaaaaaca caattatggt ttaagcatgt tccttcaaat aagttttttt
//
LOCUS       DAZUJO010000222          765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000222 DAZUJO010000000
VERSION     DAZUJO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.233"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            660..>765
                     /locus_tag="ACG8OL_003030"
     rRNA            660..>765
                     /locus_tag="ACG8OL_003030"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          315 a          124 c          134 g          192 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag
      721 tcgaacgctt ctttttccac cggagcttgc tccaccggaa aaaga
//
LOCUS       DAZUJO010000223          765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000223 DAZUJO010000000
VERSION     DAZUJO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.234"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>765)
                     /locus_tag="ACG8OL_003031"
     CDS             complement(37..>765)
                     /locus_tag="ACG8OL_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818913.1"
                     /translation="IFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQF
                     NLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDL
                     TSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLY
                     INLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQE
                     EKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          243 a          132 c          117 g          273 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaat
//
LOCUS       DAZUJO010000224          712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000224 DAZUJO010000000
VERSION     DAZUJO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.235"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..548
                     /locus_tag="ACG8OL_003032"
                     /pseudo
     CDS             258..548
                     /locus_tag="ACG8OL_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(537..712)
                     /locus_tag="ACG8OL_003033"
                     /pseudo
     CDS             complement(537..712)
                     /locus_tag="ACG8OL_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          241 a          134 c          104 g          233 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttccacggt ctgtatgaaa aagcttgacg
      601 tctgttaaag aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc
      661 tcaccacagg aataaccaat aatttctcga ttaaacaaat caagtattaa gc
//
LOCUS       DAZUJO010000225          695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000225 DAZUJO010000000
VERSION     DAZUJO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.236"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>695
                     /locus_tag="ACG8OL_003034"
     CDS             <1..>695
                     /locus_tag="ACG8OL_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS4818914.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKL
                     ALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLES
                     RFESILLAYSVLL"
BASE COUNT          235 a          127 c          123 g          210 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaatc catgaaaagc
      481 ttgcccttaa gggcattact atatccgttc cgccacgtaa aaacatggat aaatcagaaa
      541 aactagacca cagcttactt ggaaaacaaa gaaaaacagt tgagactgtt ttttcttcct
      601 tagaaaaatt aggttgtcaa aatttcaact cacgttctgt taaagggcta gagagtagat
      661 ttgaaagcat attactggct tacagtgttc tatta
//
LOCUS       DAZUJO010000226          685 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000226 DAZUJO010000000
VERSION     DAZUJO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.237"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG8OL_003035"
     CDS             complement(<1..446)
                     /locus_tag="ACG8OL_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818915.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          205 a          118 c           92 g          270 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atatt
//
LOCUS       DAZUJO010000227          628 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000227 DAZUJO010000000
VERSION     DAZUJO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.238"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          241 a           80 c           79 g          228 t
ORIGIN      
        1 aagaaggtat caagagtcac agtgaattta ttccgaaaag tgaaaagagc aaatataaaa
       61 tttaataaaa atatagaact aaataggaaa acgtagacgt aattttctac gttttccttt
      121 tcatatatat taaatattac ggaaaattat ataaactaat ttagtgtttt gtttgtaatt
      181 cctaattttg ctccactttt agataatttt taggtctatt taactcatca gacattctat
      241 gctcattttc tccaaatttg tttcaactct ttcgtactta attttgcgta tttgaaaatt
      301 cttcttttag agtactttta ttacgatact agtatgatat atatctatag caaacagtta
      361 aaatcttcct aaaataagcg aatagcattc ctaaaaccta taaaattttt agatgaagcg
      421 acaagtggac aattaataaa ctacttgaac atgaaattat ggatcacctt tcaaatttag
      481 aaaatggaat tcttatactt attgcacaac atttattgat tgcaaaatta tgtgacaaaa
      541 agaactagta gaagaaggaa taatggcttg ataataaagt actacctatt aaaggtagta
      601 ctttatttaa tttagtataa attaacat
//
LOCUS       DAZUJO010000228          622 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000228 DAZUJO010000000
VERSION     DAZUJO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.239"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8OL_003036"
     CDS             <1..608
                     /locus_tag="ACG8OL_003036"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS4818916.1"
                     /translation="EKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYS
                     NSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCI
                     RVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITT
                     KKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          249 a           74 c          107 g          192 t
ORIGIN      
        1 tggaaaaaga tcaagaaaaa tttgccagat ttagacatga ctataaaaat ttattgctga
       61 gtttaaaaga aatcgctaat caaaaccatg attcgatatt ggaaaaagaa ataaaagaat
      121 tagagatcta ttctaattct tatctaacca ataagtttga atatcgttat ttaaaaaata
      181 taaaaaatga atatttgaaa agtttgttga tagcgaagct ataccaagca aataacaata
      241 agatttgttg taaattcgaa tgtccaagta ctattattga agttcctgtt ccaatatttg
      301 actgtattag ggtgcttgga attattctcg ataatgcaat tgaagcatct atagagagtc
      361 aagatagaga actgtctcta gctatttacc gagatgataa acagatagaa atttcgatat
      421 cgaatagttg tagagagata ctactctcta tagatactct aatgaaaaaa ggaataacaa
      481 caaaaaaggg acatcaaggg ttaggtttat ctagtataga ggaaatcaat aaaaagaatc
      541 ccaatatgtt tgtgaactat caaaacgatc cgtatcaatt tactactcaa gttattttgt
      601 tgttttgagg gaaggaggat tt
//
LOCUS       DAZUJO010000229          571 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000229 DAZUJO010000000
VERSION     DAZUJO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.241"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          186 a           81 c           81 g          223 t
ORIGIN      
        1 tcttgatgtg atatccttgt cttttttgta atgtttttaa cttattgctt ttgcaaaatt
       61 tatttacctg ttttctggat caacgatagt gtacattatg ctatccttgt ttggatttta
      121 ggacgtttaa agaggcactt ttttacataa cttaatccat agacctttta agcatgctgt
      181 gagataataa taaagaagag tttaaagcgc accccaaacc acttccccat aagtgtatta
      241 cgctttaaac tcttttatat ttgaattcac taagaagcat accatatttt tgaaaaaaag
      301 tgagaaaaaa ggcttataat taggatttag agtaattaat tagatgttta ttttatagga
      361 ctgatactat agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg
      421 tgaataagat aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac
      481 ctttctggat caacgaaagt atactttata tagtcataac gccgatggta actatgcact
      541 gtaacgataa tgctgctaaa atatcttttt a
//
LOCUS       DAZUJO010000230          569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000230 DAZUJO010000000
VERSION     DAZUJO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.242"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>569
                     /locus_tag="ACG8OL_003037"
     rRNA            <1..>569
                     /locus_tag="ACG8OL_003037"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          131 c          173 g          126 t
ORIGIN      
        1 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc
       61 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      121 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg
      181 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      241 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
      301 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc
      361 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
      421 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt cagttgggca
      481 ctctagcaag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg
      541 ccccttatga cctgggctac acacgtgct
//
LOCUS       DAZUJO010000231          553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000231 DAZUJO010000000
VERSION     DAZUJO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.243"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..526
                     /gene="spxA"
                     /locus_tag="ACG8OL_003038"
     CDS             128..526
                     /gene="spxA"
                     /locus_tag="ACG8OL_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS4818917.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          196 a           87 c          110 g          160 t
ORIGIN      
        1 tttaaaaaaa ttatgaaaaa aaggtagcta tttaaaagtt gattatgtat aatagaaaca
       61 gatgggagat tattatgatc ttctgttaca aaaaagtgat tcttatcacg aaaaggagtg
      121 gtgcacgttg ttaacattat atacttcccc aagttgtact tcctgcagaa aggctcgtgc
      181 gtggttgcaa gaacatcaga ttccatttgt tgaacgtaat attttttcag aaccattgaa
      241 cagttctgaa ttaaaagcga ttttacaaat gacagaagac ggaacagaag aaatcatctc
      301 gacacgttcc aaagtattcc aaaaactgaa catggattta gatgatctgc cgttacaaga
      361 attattggaa cttgtgcaaa acaatcctgg attgcttcgc cgtccaatca tgattgatga
      421 taaacgtctg caagttggtt ttaatgaaga tgaaattcgt cggttcctac cgcgcgatgt
      481 acgacaatta gaattacgtc aagcacaatt gatggctgga ttatagaagg ataatacgaa
      541 acttggaaaa gac
//
LOCUS       DAZUJO010000232          534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000232 DAZUJO010000000
VERSION     DAZUJO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.244"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACG8OL_003039"
     CDS             <1..>534
                     /locus_tag="ACG8OL_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS4818918.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZUJO010000233          534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000233 DAZUJO010000000
VERSION     DAZUJO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.245"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          189 a           82 c           86 g          177 t
ORIGIN      
        1 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttatatt
       61 gtgagataat aataaagaag agtttaaagc gttccccaaa aaccactgcc ccataagtgt
      121 gttacgcttt aaactctttt atatttgaag ccattaaaaa gcataccata tttttgaaaa
      181 aaagtgagaa aaaaggctta caattggagt ggtagttaat tagtgactta tttttgattt
      241 tatagcactg atactataaa atatagatat catcatatta cacaatctta atactaactt
      301 aaaaaatatc tcctttcaca agtatggtga taaaattcgt tccgggctac ctttttaggt
      361 agcctacttt aatctttata cctttctgga tcaacgaaag tatactttat atagtcataa
      421 cgccgatgat cgctacgtgc gtccggcacg tcagtcatga atcggctaca ctagactaga
      481 cagaaaaaat aaggtgtgta gaataataaa aaacacactg gaggattttt catg
//
LOCUS       DAZUJO010000234          525 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000234 DAZUJO010000000
VERSION     DAZUJO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.246"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..>525)
                     /locus_tag="ACG8OL_003040"
     CDS             complement(116..>525)
                     /locus_tag="ACG8OL_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFS4818919.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          166 a          104 c           80 g          175 t
ORIGIN      
        1 actgtagata tcatccataa aatggacttt gtctacagtc tgagacacga cctaaagatc
       61 gtgtctaatt atgactgtat cagaaaatat gataccaaac ggatctattc attaattaat
      121 tgacatagtt tatctgttgc tcttttatga ctggataact gaccaattca tgacgatcca
      181 agtcttcttt attcatattt acagcagtaa tttgactgtc ttcatacgta cgataataat
      241 aaatcccttt atcaacgtta cagcaagaag aataaatcgt atattcgtat tttccttctc
      301 cagtatcgca tactcctttt tgttgctcga ctgagcctag tatatgaaaa aattgactga
      361 tactttcaga ttctgaatca cctgatgcag cattcatttt agtaaatgtg gcttttacaa
      421 atcgagaaac agaagagaga tctccaggta agcccaatcc tcccatccca cgactataaa
      481 cattcaagct gatctggttt gaaaaatgat tttctggtgt tccat
//
LOCUS       DAZUJO010000235          514 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000235 DAZUJO010000000
VERSION     DAZUJO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.247"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8OL_003041"
     CDS             complement(<1..132)
                     /locus_tag="ACG8OL_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS4818920.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZUJO010000236          482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000236 DAZUJO010000000
VERSION     DAZUJO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.248"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8OL_003042"
     CDS             complement(<1..430)
                     /locus_tag="ACG8OL_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4818921.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          136 a           96 c           64 g          186 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tc
//
LOCUS       DAZUJO010000237          476 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000237 DAZUJO010000000
VERSION     DAZUJO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.250"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..>476
                     /locus_tag="ACG8OL_003043"
     CDS             125..>476
                     /locus_tag="ACG8OL_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS4818922.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIF"
BASE COUNT          180 a           78 c           86 g          132 t
ORIGIN      
        1 ccgcactatg ccattacatt tatatctacg cgaattgtaa aattaagtta gaaaaataaa
       61 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
      121 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
      181 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
      241 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
      301 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
      361 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
      421 gtctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa tatttg
//
LOCUS       DAZUJO010000238          472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000238 DAZUJO010000000
VERSION     DAZUJO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.251"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            263..>472
                     /locus_tag="ACG8OL_003044"
     CDS             263..>472
                     /locus_tag="ACG8OL_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818923.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRT"
BASE COUNT          175 a           82 c           72 g          143 t
ORIGIN      
        1 tctaactcat atgtgaatga tttataaaat atcccaacgt ctcatctaaa gaaatgttga
       61 tcgtcagcaa ggagctaaaa aatacgaata tatttctttt gtagtaaaat cggactggtt
      121 tgcatatcat tttcttcgta aatatcaaaa tcatgttaac tactatcaat tactaataaa
      181 aactaccact ttaagcgtta agaactgtta agtttagtta agtgaggata gttcacaaaa
      241 tccattgtta aacttcacga aagtggtaaa actcaaaatg ctttatatag gattacagca
      301 tcgctctttc ttcgattgcg cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga
      361 cacgttcctt acggctcatc agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga
      421 aaatttaatt ctaaaaaagc gattgccata ttcacgcctc gctcgacgaa ct
//
LOCUS       DAZUJO010000239          448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000239 DAZUJO010000000
VERSION     DAZUJO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.253"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..>448
                     /locus_tag="ACG8OL_003045"
     CDS             35..>448
                     /locus_tag="ACG8OL_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818924.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFV"
BASE COUNT          130 a           82 c           91 g          145 t
ORIGIN      
        1 agatttatga aaacgaatgc aaggagtgat agctatgatg tctaaaaaca atacgaatgg
       61 tagaaatcaa tttgcgatgc ttacgattga tgatctagtt cctcaagatc atctcgttcg
      121 aaagattgat gccgcgcttg attttgaatt tatttatcca atcgtcgaag ccacttactc
      181 cgatttaggt cgcccaagta tcgatccagt gattctgatc aaattggtgt ttattcagta
      241 tttattcggt atccgttcca tgcgacaaac cattaaggaa gtcgatacca atgtcgctta
      301 ccgctggttt ttaggctact ccttcgagga gaaaatcccc catttctcaa cttttggaaa
      361 gaattatgtt cgccgattta gagaaacgac ggtgtttgag gatatttttg cctatatttt
      421 agaacaagca gttaaagcag gttttgta
//
LOCUS       DAZUJO010000240          442 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000240 DAZUJO010000000
VERSION     DAZUJO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.254"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACG8OL_003046"
     CDS             complement(<1..190)
                     /locus_tag="ACG8OL_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS4818925.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGK"
     gene            complement(209..370)
                     /locus_tag="ACG8OL_003047"
     CDS             complement(209..370)
                     /locus_tag="ACG8OL_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS4818926.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          146 a           79 c           47 g          170 t
ORIGIN      
        1 atttaccatc tttttttgtt attgatattg aagaattaga actttcatat aaagcctttt
       61 ctatttcatt gcttgatttc attattgtta aacaagaata caagcaagaa agaactatag
      121 ttaatatgat aaaaattatt aaagttctat ttttctttct tgtaatatat gctatagcat
      181 tttttatcac tttcctaact actcctctct attttcttac tccataacta attgcatcca
      241 ctggacaagc atttttacat cgtccacatc tgatacactc cagatgattg caattctcaa
      301 ctgaatcttt attcatttgg caaacttttt tacattttcc acaattaata cacttatcct
      361 tatcctgaat cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa
      421 acacactgga ggatttttca tg
//
LOCUS       DAZUJO010000241          423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000241 DAZUJO010000000
VERSION     DAZUJO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.255"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>423)
                     /locus_tag="ACG8OL_003048"
     rRNA            complement(<1..>423)
                     /locus_tag="ACG8OL_003048"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           85 a          132 c           98 g          108 t
ORIGIN      
        1 gttgagccgg gggctttcac atcagactta agaaaccgcc tgcgctcgct ttacgcccaa
       61 taaatccgga caacgcttgc cacctacgta ttaccgcggc tgctggcacg tagttagccg
      121 tggctttctg gttagatacc gtcaagggat gaacagttac tctcatcctt gttcttctct
      181 aacaacagag ttttacgatc cgaaaacctt cttcactcac gcggcgttgc tcggtcagac
      241 tttcgtccat tgccgaagat tccctactgc tgcctcccgt aggagtttgg gccgtgtctc
      301 agtcccaatg tggccgatca ccctctcagg tcggctatgc atcgtggcct tggtgagccg
      361 ttacctcacc aactagctaa tgcaccgcgg gtccatccat cagcgacacc cgaaagcgcc
      421 ttt
//
LOCUS       DAZUJO010000242          418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000242 DAZUJO010000000
VERSION     DAZUJO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.256"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>418
                     /locus_tag="ACG8OL_003049"
     CDS             <1..>418
                     /locus_tag="ACG8OL_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818927.1"
                     /translation="HLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKT
                     EYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYG
                     HHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMD"
BASE COUNT          154 a           76 c           70 g          118 t
ORIGIN      
        1 gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt ttttagatta
       61 cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt cttactttcg
      121 atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc gtaatggtga
      181 gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg aaaccactat
      241 tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga tcgaaaaaat
      301 gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc tgactgaaga
      361 agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt ttatggac
//
LOCUS       DAZUJO010000243          368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000243 DAZUJO010000000
VERSION     DAZUJO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.258"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG8OL_003050"
     CDS             complement(<1..272)
                     /locus_tag="ACG8OL_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818928.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAZUJO010000244          323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000244 DAZUJO010000000
VERSION     DAZUJO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.259"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..>323)
                     /locus_tag="ACG8OL_003051"
     CDS             complement(44..>323)
                     /locus_tag="ACG8OL_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS4818929.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           88 a           81 c           60 g           94 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt cca
//
LOCUS       DAZUJO010000245          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000245 DAZUJO010000000
VERSION     DAZUJO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.260"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG8OL_003052"
     CDS             <1..>321
                     /locus_tag="ACG8OL_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818930.1"
                     /translation="KNYPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFM
                     IQSGESEETWTTFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLR
                     NIFT"
BASE COUNT          117 a           62 c           60 g           82 t
ORIGIN      
        1 aaaaaaatta tccttactta atgaccgatg tactctatat aaaagtacga gaagaaaatc
       61 gagtactctc aaaaagctgt catatagcga ttggaataac caaagatggc gaccgtgaaa
      121 ttatcggctt catgattcaa agtggcgaaa gcgaagagac ctggacaaca ttttttgaat
      181 acctaaaaga acgcggttta caaggtacgg aactcgttat ttctgatgcg cacaaaggat
      241 tagtctctgc cattagaaaa tccttcacca acgtaagttg gcaaagatgc caagttcact
      301 tcctaagaaa tatctttacc a
//
LOCUS       DAZUJO010000246          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000246 DAZUJO010000000
VERSION     DAZUJO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.261"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8OL_003053"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8OL_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818931.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZUJO010000247          280 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000247 DAZUJO010000000
VERSION     DAZUJO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.denovo.263"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..131
                     /locus_tag="ACG8OL_003054"
     CDS             <1..131
                     /locus_tag="ACG8OL_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS4818932.1"
                     /translation="RVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT           83 a           58 c           57 g           82 t
ORIGIN      
        1 aacgagtgat tcggattttt ccaaatatca attccatcac tcggataatt ggaacacttt
       61 tgatggaaaa agacactgag tggctggctt ctccacgaaa atatttagaa tttaattcga
      121 ataacattta gcaccatgaa cctgtgcgag tctcttcttt ctgaggagag acatcaagag
      181 tcgctgcgct actgctcctg acctctctcc tcagaaagaa aggcatggca gttaggttca
      241 caacgcaaaa cattgtgctt ttgatgaggt tgaactttta
//
LOCUS       DAZUJO010000248        22715 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.042, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000248 DAZUJO010000000
VERSION     DAZUJO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.042"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(282..1562)
                     /locus_tag="ACG8OL_003055"
     CDS             complement(282..1562)
                     /locus_tag="ACG8OL_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS4818933.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(1606..3639)
                     /locus_tag="ACG8OL_003056"
     CDS             complement(1606..3639)
                     /locus_tag="ACG8OL_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818934.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(3673..4524)
                     /gene="rfbD"
                     /locus_tag="ACG8OL_003057"
     CDS             complement(3673..4524)
                     /gene="rfbD"
                     /locus_tag="ACG8OL_003057"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS4818935.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(4622..5650)
                     /gene="rfbB"
                     /locus_tag="ACG8OL_003058"
     CDS             complement(4622..5650)
                     /gene="rfbB"
                     /locus_tag="ACG8OL_003058"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS4818936.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(5672..6244)
                     /gene="rfbC"
                     /locus_tag="ACG8OL_003059"
     CDS             complement(5672..6244)
                     /gene="rfbC"
                     /locus_tag="ACG8OL_003059"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS4818937.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(6258..7124)
                     /gene="rfbA"
                     /locus_tag="ACG8OL_003060"
     CDS             complement(6258..7124)
                     /gene="rfbA"
                     /locus_tag="ACG8OL_003060"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS4818938.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(7224..7931)
                     /locus_tag="ACG8OL_003061"
     CDS             complement(7224..7931)
                     /locus_tag="ACG8OL_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS4818939.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(7936..8763)
                     /locus_tag="ACG8OL_003062"
     CDS             complement(7936..8763)
                     /locus_tag="ACG8OL_003062"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4818940.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(8763..9563)
                     /locus_tag="ACG8OL_003063"
     CDS             complement(8763..9563)
                     /locus_tag="ACG8OL_003063"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS4818941.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(9556..10695)
                     /locus_tag="ACG8OL_003064"
     CDS             complement(9556..10695)
                     /locus_tag="ACG8OL_003064"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS4818942.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(10900..12093)
                     /locus_tag="ACG8OL_003065"
     CDS             complement(10900..12093)
                     /locus_tag="ACG8OL_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS4818943.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(12172..12378)
                     /locus_tag="ACG8OL_003066"
     CDS             complement(12172..12378)
                     /locus_tag="ACG8OL_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS4818944.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(12371..13513)
                     /locus_tag="ACG8OL_003067"
     CDS             complement(12371..13513)
                     /locus_tag="ACG8OL_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS4818945.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            14025..14660
                     /locus_tag="ACG8OL_003068"
     CDS             14025..14660
                     /locus_tag="ACG8OL_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4818946.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            14796..14888
                     /locus_tag="ACG8OL_003069"
                     /pseudo
     CDS             14796..14888
                     /locus_tag="ACG8OL_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            15002..15637
                     /locus_tag="ACG8OL_003070"
     CDS             15002..15637
                     /locus_tag="ACG8OL_003070"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4818947.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            15744..16982
                     /gene="mef(H)"
                     /locus_tag="ACG8OL_003071"
                     /gene_synonym="mefH"
     CDS             15744..16982
                     /gene="mef(H)"
                     /locus_tag="ACG8OL_003071"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFS4818948.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            17209..17628
                     /locus_tag="ACG8OL_003072"
     CDS             17209..17628
                     /locus_tag="ACG8OL_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS4818949.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            17633..17872
                     /locus_tag="ACG8OL_003073"
     CDS             17633..17872
                     /locus_tag="ACG8OL_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818950.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(18097..19020)
                     /locus_tag="ACG8OL_003074"
     CDS             complement(18097..19020)
                     /locus_tag="ACG8OL_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS4818951.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(19052..19816)
                     /gene="map"
                     /locus_tag="ACG8OL_003075"
     CDS             complement(19052..19816)
                     /gene="map"
                     /locus_tag="ACG8OL_003075"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS4818952.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            19974..20414
                     /locus_tag="ACG8OL_003076"
     CDS             19974..20414
                     /locus_tag="ACG8OL_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS4818953.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(20592..21161)
                     /locus_tag="ACG8OL_003077"
     CDS             complement(20592..21161)
                     /locus_tag="ACG8OL_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS4818954.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            21416..22648
                     /locus_tag="ACG8OL_003078"
     CDS             21416..22648
                     /locus_tag="ACG8OL_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS4818955.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         7003 a         4493 c         4047 g         7172 t
ORIGIN      
        1 gatcgaaaac tgtagcaata attttctgtt ctgataaatg tccttcataa ataaatacaa
       61 atctttaaac atttgtgttc ctccaagcga ttttcagtga attacagtca agtgccattt
      121 taccatattt ttggtaccga gctgcgttct tatttctttc ttaattttct atgatacaag
      181 attttctcta acgtttctat tttacagaag atgctagaca attgcaataa accaaaaaag
      241 gctgcgacgg ttgtcacagc ctctctcgta ttttgtgcct atcaatgata gcgttggatc
      301 ctggaagaat gagcgctatc tggttgttcc tcataagatg tatcatcata agtcatatcg
      361 tcatatgcct cttccataac atagaattcc tccgcaactt ctggttcatc atatcgatga
      421 acggccatag ccaacaggat ataaataatc ggaacccatg ggtatgaagt caatgtatcc
      481 tccataaatg actgcatcat caccgcaaaa aaggcccaga taaaagtcat ctgcccgttg
      541 gataaatctg ctttcaacgt taagcgacca ataaaaagga taagcagcaa agcagcaatc
      601 ccaaatccta tcaatccata gatattcaaa atagaattat aactattgtg gtttcctaaa
      661 aattggtttc ctcgttggaa gataaatgtt ttcattccta gtaaaatttg ttcagatttt
      721 tctcctagcg tctgatagaa tttatgccaa atatcttctc gaccggtaaa caaattgact
      781 ttttcagaga cagccgccag ataagaaatc ggcaaagcta aaagaatcgt aaccgtgtaa
      841 gcaataaatg gcgcacgaat gacacgcatc aacgatttag gtaaaagatc caatatcgca
      901 aaaacaatca acgcgactaa tgcacctcta gattggcaga cccaaatccc gagaaaagct
      961 gcaacaactg ctactgcacc gatgattcgt acataccatc gttcaaaaga actggcaaat
     1021 ccactgatca ataaagcaag agtcatcaaa gaacttccga ttatattcgt attgatccaa
     1081 atatcagaaa gcgaattgcc tggagcccag atgctttgtc cttctggtac ttggatctct
     1141 gtaaatacac gatataacgt gattccgagg aagaccgaaa agacaatcag ataaataccg
     1201 cgatcccatt ttgtccactc gatccattta attactaaac aaaataaaat aatcaaagga
     1261 aacagtaagt ctccaaataa agactcattt cttaattccc gaaagtaaga aaagaaaatg
     1321 aaaagggcaa gcgcaacacc tagataaaga aaatcgacgc cttttgcttt ataaacattt
     1381 aaaatgagca aaatagccac actaaaagcg ataagtaatg aactattgtt atatataaac
     1441 aaagttgctg gaaaacctaa ccctgctgtc caaacaaaaa agactagcgt cagtaacaaa
     1501 agcaagaaac cgacactcat tttgttgaca aatttttgta cgattgcttg aattatctcc
     1561 actctccttt ctactaacat ttcaggatta ttctcagcta gcctattact gtacgactag
     1621 ctgattttcc tgagtttgtg tttgtggtac atctggataa tccggatatt ctactttaaa
     1681 aatcatataa ccgtctttat ccggtccata tagctgcgtg aactgcgctt catataattg
     1741 gttgtaatca gttaatggtc gtttactgat cacatagtcg atccctaatt tttctgcgtc
     1801 ttcaaaattc aacgtgaccg aaaacatatc tggacgatct aaaatgaact ttgtatcttc
     1861 cttagcgata aaagctcttg tatgagcata ccgattataa tacttctcgt attcttcttt
     1921 cggatcgatt ttcttccatg cagctatatc tggataaaag cgtactgtat tgaaggattt
     1981 cactcccaat gttggggtga agttatataa ttcatcttca gacaaccaaa ctttaccagg
     2041 atcttgttga tcgatttttt ggatttcttt tgccagtgtt ttttcaaaaa tcgctcctgt
     2101 accttctacg actggattga cgaaaaagcc agatacgata actaaggcgg caaaagcgag
     2161 gtaaaacaag cgtctccagc caaataatgc aaaagctaaa atcactgttc ccaacacagc
     2221 aactgcgatg gtttcaaaat ccgaaaaata attatccatt ttcgaatgcg taatcgcata
     2281 gaaataagac catgcattta ctgccaacaa aatgaattta taccaaatag gaataacatc
     2341 ttctctccaa atataagcga taaaccagat cgataataaa caagccgcaa aactaaaagt
     2401 aaggatggct cttaatggag gaacataaga cagcagagtg atttctgcta gccattttgg
     2461 caacccaacg gtaatccaaa agatagaaaa cagacaaaaa atcatcaaaa tacgtcccag
     2521 taacttttgt tcgctatctt ttttgccaaa taacacaaat ggacttgcta ggaatacagt
     2581 tggaaaaaag tgatagaaca gggccacttc agagttattc gaatagttga tatcttcaaa
     2641 aggaattttc caatttgtaa ggaaagaaaa aaattcgcca atcgtccact ttcctccagt
     2701 acttacccgt tttcctggat ataaggtatt caaagaagct aacaatgcgt cttttgacgt
     2761 tagccagaaa tgaaccatga ctccgccaat aaataaaact gcccctccaa tcaacactac
     2821 atccaacaga tcccatgaaa ctttcttgct atagtaaata agcaggccaa caaaataaaa
     2881 aatcagcaag tacgccagca tcacttgatg ggctgggtaa atcactaaaa taaacccaat
     2941 accgaaaata accaccaaaa gcatcatcaa cgttcgtagc cattttttat catgattgta
     3001 gaaataatga taaaaagcag tcatcagtcc caaagtaaag aaaatcaaat cgcccacatg
     3061 ttgcatgaac caccactgga ctgctggaga aaatgtcagc gcaaatgcac cgattagcga
     3121 tagagctttt tttctcttcg ttaatatcat cagtacttcg aatccaagca agaccatgac
     3181 gactaatttc atcgcccaat aaaaggaaag tcctcgatct cttccgagga aaaagaatcc
     3241 ccagttgaat ggttttccga taaccgtaat atcctttaca ggagaattat acgcaatgat
     3301 catgttttgt ccgtttaatg aataatcctc gttcaccaac ggaaagtcct cttcagcttg
     3361 tgagaggtaa aaaggtgtct gcaccagcca ttcgtctgac cgcacttccc gattctgtcc
     3421 aaaaatcaca tcagtttttt tcccatcgtc tctttgcgac acatatttat cccaaacacc
     3481 aagtgaactt ccgtttaaat taaaagtaac gatgaccacg atgactaaaa cgccaataag
     3541 atagcggagt tttagaaaca ggtcaagcac caaattcgaa tctgcctggt tcgcttttgg
     3601 cttttctttt cgtcgtgcac ttcttgtttg tttttccata tcatgctcca aattctattt
     3661 gataaaagga aattacttat tcattttttt ggcagcttcc agcatttgcg caagtgcttc
     3721 ttgccaagtt ggtatgacaa atcctaactc ttctgtcttt ttcaggctca tgactgaata
     3781 ctgcggacgt gttgcttttt gcggaaattc ttctgacgta acaggtgcaa cttctacatc
     3841 tgtatctttc aatatttctt ttgcgaattc ataccaagaa cagctatttt cgttcgacaa
     3901 atgataaata ccaaatggtg ctttttcttc gatgacatat accataaatt ctgccaaagt
     3961 acgtgtccaa gttggtcgcc caaactggtc atttactaca gtcagtttgt cgcgggtttc
     4021 cgctaggcgt tgcatcgtga agacgaaatt gtgtccatag atcccgaaaa cccaagaagt
     4081 tcgtatgata taatagtctt ctaaaatctc ttctactgct ttttcgccta ataatttcgt
     4141 acgaccatat tcatttagtg gattcgtttg gtcgtctact tcgtattcta catttttgtt
     4201 ttttccatcg aaaacatagt ctgtactgat atagacgaat tgagcattca cagcttttgc
     4261 tgcaagtgct acatttcttg taccatcaac attgatcttc tcatctaatt cttttgcttc
     4321 ttcttctgct ttatctactg ctgtgtaagc tgcacaatgg aaaataacat caggttttaa
     4381 ttctgtgatg taagccattg ttttctcggc atctgtgata tccaagtctt ttgcatctgt
     4441 tgaaacatat tgcagatttt tttcatctaa taaatggcgc aattctgtac ctagttgccc
     4501 atttcctcca gttaataaaa acatatgatt atccgctcct tgatttttat tttaaaaaag
     4561 agacaggcga gcgcctgcct cacatgtgtt tttccattaa tacagtgaca ttttctctgt
     4621 attattgtcc atttttggca tatgctgctt caacggcctc tttttctttt ttccaccagt
     4681 cttcatgttc ttcgtaccat ttgatcgtgt cagccaaccc atcacggaag ttcgtaaact
     4741 caggcttcca tcctaactct tcccgtaatt ttgtcgaatc gattgcataa cgcaagtcgt
     4801 gtcctgcacg gtcgtttaca tggtcgtacg catcttttgg ttgacccatc atttctaaga
     4861 tcaattccat cacggtctta ttatcttctt caccgtctgc accgatcaga taggtttcgc
     4921 caattctgcc tttcgtcaaa atcagccaca ctgctgaaga atgatcattc gtatggatcc
     4981 agtcacgaac attctttcct tcgccatata attttggacg aataccgctt aagatattcg
     5041 ttacttggcg aggaataaat ttttcaatat gctgataagg accatagttg tttgaacagt
     5101 ttgaaatggt tgcacgtaag ccaaaagaac gcacccatgc ttttactaac agatctgaac
     5161 cagcttttgt agaagaatat ggacttgaag gattatatgg tgtttccgca gtgaattttt
     5221 ctccttcgcc ttccccatgt cctggcaggt cttcacgtaa tggaagatcg ccgtaaacct
     5281 cgtcagtaga tacgtgatga tagcgtacat catacttgcg gcaagcttct aaaagtgtat
     5341 acgtaccaat gatattcgtt tgtacaaatg ggaaaggatc ttttaatgaa ttatcgttat
     5401 gggattctgc cgcgtaatga acaactgcgt ccgcttcttt tactagacgg tccactaatt
     5461 ctgcatctgc aacatctcct acgaccaatt ctacgcgatc gctaggaagt ccagccaagt
     5521 tctctttgtt tcctgcataa gtcagtttat ccaaaacagt gacatgcact tctggatgat
     5581 tgtttaccac gtaatgaacg aaattagagc cgatgaatcc ggcacctccg gtaacaatga
     5641 tgtttttcat aagaaaattt ctccttaatt cttagatttc tccgtaaaca aatggatttt
     5701 ctgcttcaaa ttcttttaat gtcggatgac ggtgatcttt ttccgacata atcgctcttg
     5761 atggatcaat tggccaatca atcgctaaat ctggatcatc aaatgcgatg cccccatctg
     5821 cttctgcatt atagtaatta tcacacttat aaaggaagtt cacatttggt gtcaaagtaa
     5881 cgaatccatg ggcatatcct ttaggtacaa gcaactgtcg atgattatgt tcagataaga
     5941 taaatccgtc ccattgacca taagtcgggc tacctttgcg gatatccact actacatcta
     6001 aaactgctcc agtgactacc cgaatcaatt ttgtctgtgc agcttctcct ttttggaaat
     6061 gaagcccccg aaggacacca gcttcagctg atagtgaatg gttgtcttga acaaagtcat
     6121 atttcaatcc atgttctgca aattttgctt ttgaataact ttctgtgaag aaaccgcgat
     6181 gatcgccaaa gacgtccatt tctacgattt tgacatctgt taattttgta tctgttactt
     6241 tcatctgtaa ggactcctta ttcagcggcc aaattcaata aatattgacc gtattgattt
     6301 tttttcaatg gctgtgcaag ggccagcaat tgttccttat cgatgtagcc catacggtaa
     6361 gcaatttctt ccaaacaagc cacttttaag ttctgacgtt tttcaattgt ttcgataaag
     6421 gtagatgctt caagcaatga ttcatgtgtt cctgtatcaa gccaagcaaa tccgcgtccc
     6481 ataagttcta cggatagttt tcctttatcc aaatatgctt tattgacatc cgtaatctct
     6541 agttctccac gttctgaagg tttgatacct ttagcgatag aaattacctc attgtcataa
     6601 aaataaaggc cagttacagc gtaatttgat tttggctgag caggcttttc ttcaatcgac
     6661 aaagcttgca tattctcatc aaattctact acaccaaagc gttctggatc attgacatga
     6721 tagccaaata ctgtcgcgcc tgtatctttt gaagctgctc gttgcaacat tttagacaga
     6781 ccgccaccat agtaaatatt gtcccctaat accaagcaga cactatcatc tccaataaat
     6841 tcttctccaa tgataaacgc ttgtgccaat ccatctggac tttcttgtac tgcatattcg
     6901 atctgcagtc ctaaatcatt tccgtttcca aataattcct tgaaacgtgg tgtatcttgt
     6961 ggcgttgaaa taatcagaat ttctttgatt cccgccaaca tcagagtaga cattggataa
     7021 taaatcattg gtttgtcata aatcggcatc aattgtttcg atgttgcctt tgttaaaggg
     7081 tacagacgtg ttccgctgcc cccagcaaga atgattccct tcatggatat ctgctctcct
     7141 ttttgatttc aaatttcact ttgttattat ctcatcaatt gttatatttg tcatggaaat
     7201 attaaggatt taaacatctt ttatcaacgt ttaaaccatt ttcgtttgat caacgccgta
     7261 atcagatgaa cacgccaaga aattttttgc cgtaatttag aatgtgtttc aatctcactc
     7321 gcgtttaaat catgtcttcg gtacagagtt aacggttctc ttaggaaccc agtcttcttt
     7381 cctcccagca aaccaatcca catatcatgc atcggcactt ctgacgggat cggcaaaata
     7441 tcgggtttta aagaagaccg gaaagccata ccagcaccaa tatagtgact tcttatcaca
     7501 ttattaagcc atccttcttt ggaatttcga aacgaaaaat acgaatcatg gatctcttgc
     7561 agctcgctgt ccacaatgat taaatcagaa actactacta gctgatccgg attctgctca
     7621 aaatagtcag tgatcttttt cacttttgtc tccaaccaaa catcatcttg gtctgccaaa
     7681 aagattagat ctcctttcgt ctgctggatc gcataaccaa aattagcgat caatccttga
     7741 cgaggccccg aaaaaagctg gattcgctta tctttttctg cgtattcagt taatatatcc
     7801 catgtatcat ctactgaccc atcatcagaa atgactactt catcttctgc tgaaagctga
     7861 gataaaatac tatctaactg ctctcttaaa tatctgcctc cattaaaggt agctatacaa
     7921 accgaacgca cgaattcaca ccttccaatt cttaacatat gagcgcaaag tcatccgcca
     7981 gatatgccgg tttttgacgg tcaagaactg tttagctgtt cttaacaata attgtcgtcg
     8041 gtgactggat aaatgccttt gatcatattc atgataaaat cgattttctg cttgtaaaat
     8101 agaacggtat cgattacttg aaactttttc atagtctaaa accgaaagat catgttccat
     8161 tcgtacagga aggatctcca cagttttttc caactgatcc actttccaaa acagccaatg
     8221 atccaaaaaa tctaaagaaa aagcaggatt aaacccgcca atctttttta gaaatttgac
     8281 agaccaggca acacctgaat tgattgccat gattcgttca gctgttgggc cttcaacttc
     8341 tactgcttga gctgtacgat tgatataacg atctgaatat aggggagaaa cttgtcggct
     8401 cccagaaaaa atcatcggaa ctgcagcaac acattcatct gtaaaggtta ctttacgcaa
     8461 tagttcaaaa taatcgtcga ctagcctcgt atcctgatcc agagtgacga gacaacgtgt
     8521 tccttcatct gcttgagaca acgcaaaatt ataagccacc gctaaaccag gattttgcgg
     8581 atcatgatga taaacgatat tttctttttc aaacagctca ttttgctgcc tttgaggact
     8641 attatcgtaa ataataagct gaatttgctt ttcgctaagt gctttgctta atggtccata
     8701 agctggagat tgactgaata gttgctgata aagcacaacg actactaata tttctggttt
     8761 catcagaaaa aacgccaccc ccacttattg aaaaatttca tcatagaagt caaaaacacg
     8821 cgaaacagtt ttgcatttcg atgcgcaccc ttcccgtaaa aatggataac tgcataaaag
     8881 ggtgtgtaca ataacttgta acctgctttt tcaaaactca aacacaagtc gttgtcttca
     8941 aagtacatga agaaacgctc atcaaacccc tttatctctt taaatttatc aatatcagct
     9001 accataaaac tacctgagat catccgtaca taactattag catcagtagg caatcctcgg
     9061 cattcaaaca cagccagtcg tttgtcaaac ttttttttga cccatttgaa aggcacaaag
     9121 cgcaaaacgt aatcaaaaac agccagctgc tcacggatga ggtgctgagt tgttccatct
     9181 tcattaagaa tcttaggtgc taacatcgca cattccggat gttctttcaa taacaagacc
     9241 aatgctgtaa ctgtctcttc atccactaaa atatctgggt tacaaataat gccatagcga
     9301 gaatcgctag ctatcagatt gtgattatgt ccataaccaa atccggaatt ttcatcatga
     9361 aaatagatat cgatgtaggg atatttccta tactctgtta gtcttttttt gtactcggga
     9421 agtgaattgt tgtcaaaaat tcggattttt actgctgttt catcttttac agttgaaata
     9481 aaattatcta gtgtccgaaa aattttatca ctgtttgccg tcacaatact aataaggatc
     9541 ggctggttta atttgttatt catctatatg acggttccat ttcttcaaat atttctgttt
     9601 tacttgcgta cggtactctt tattccctaa aaatcgcaat gtttttaaaa taaactgatg
     9661 tttctcattg atcagaccaa tcaattcaaa taataaaatt gcagcaaaca acatggcaat
     9721 aatcaatatg atcgttccga ttcttccagt ataattcatg accatagctg tgaaagaaaa
     9781 catcaaagcc atcgcataaa tcgtcaggac agcaccttta tgagtaaagc ctaaactgat
     9841 tagtcggtga tgaagatgtt ttttatctgc tgtagaaaaa gaaacacggt ttgcttttct
     9901 cctaatgata gcaaaaaacg tgtctgttac cggcactccc atgacgatca gcaaagaaat
     9961 cgatgaaaca aaagtcacgt tcttcagccc ttgtagtgaa aggacggcca tcataaaccc
    10021 taggaataga gctcctgtat cgcctaaata aatctttgcc ggataaaaat tgtaaggaaa
    10081 aaaaccgata atacttgcta caagacagaa aataacaact ggaatataca ctgtagaagc
    10141 atggaggaaa aaatatccaa tgattcctat cgtaattaaa ccgatcaatg aaatccctgc
    10201 cgccagccca tccaaaccgt caatcagatt gaccgcattc gtgataccga agatccaaaa
    10261 aatcgttacg ggaaaactaa gccagcctag ttggatatat ccaataaaag gaagcgtgaa
    10321 ggaatcaata cgtatatctg ctacaaaata aataataaga gaagcaatca agatccctat
    10381 acttttttgt ttaggtgtca gctcatattt atcatcgagc actcctgtga ctatgattac
    10441 taaagaagca atcaagatcg gtacaatata cgattgaggg ataattttac ggaataagaa
    10501 taaagtcgat ataacaaatg aaataaagat aggcagtccg cccgctgaag gcattggaac
    10561 tttatttatt cttctttcat ttggtttgtc gaccatacct gtttgtactg aaatgatttt
    10621 gaatacaggt gttaatatca gtgataataa aattgtcaga aaaaatctta taaccatact
    10681 aaaagaaaca agcatatggg cacctacttt aattaagagt cataattatt ataaattata
    10741 tatgtctttt tgactatctt tttatatctt ctcaaatgtc cgcaaagcct tattttatcg
    10801 gctctacggg cattttgctt ttgtggtaaa cctcgcatat ctaggtctat cttcttatat
    10861 tttccctgtc aatcgtggtt aaaatcgtgg taaatacttc tatgcgatta gacgctgaac
    10921 ctctgatttt gcggtatcta tggacgcatg agcgtaccag ttcattgtga tactgatgtt
    10981 tgaatgcccc atgatatact gtaaatcttt cgggttcatg ttcttgcttg ccagccttgt
    11041 gcagaatgta tggcgtagcg tatgcggtgt aatatgtggc aagggattgt ccttgtggtg
    11101 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatgg caacttttgg
    11161 attacctttc ggattgataa acacaaaacc gctgtgtccg tctacctcta tcgactttat
    11221 ctttggtcgg ctgttgataa cccgttgaaa tgcctgtatc gtttctttgc ttaatggcac
    11281 ttgccttatc ccgcttttcg tcttaggcgt ttcaatataa tagccctgtt ccttactctt
    11341 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    11401 tccgcacagt tccgagatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    11461 cttgtgatac acgttgtccg tcttaataaa tgacaataag gcttgctcct gttcctctgt
    11521 caatgcgact ttctctttgg tatcattttc tatgacttca cttagcttga aatcaaaagg
    11581 gtttttcctc acataatcat cttgtatggc tatgtagaat gacgctttta acgagcgttt
    11641 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcttt
    11701 agcgtcagag ggttttatcg tatcaatgct cctagcacct aacttgtcct cttttaataa
    11761 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgtcc tctgtgcgtt
    11821 ctgtttggca tagagttggc aaagcgtcat tttcttgcct gtgctgtcga taccgtcctc
    11881 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    11941 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttcccaatgc
    12001 gtctacatat ttatataagt attttccgtc tgttcgttgg ctctctccag tatgcaggat
    12061 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    12121 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagattgc
    12181 gtccaattta tcaataacct tttcaaactg tcggcgttta atctgtatgc ggttgccatt
    12241 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    12301 aatgcggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    12361 atcctgtgta ttactcataa aacaccccct cactttcgat tacgtctgat atgtccgtac
    12421 acatctgttc cacaagcatt tcctgttttt cagaaaggtg ggtacattca tcaatcattt
    12481 tcatgtagtt tgaacctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    12541 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacaggg ttcatttcaa
    12601 aaggtaaact gtcatgggaa atgaatatct cccacaatgg atagtcagga aattccacaa
    12661 aatcggtaat gagataaaac accagcccat gagggttata cgtcaataac agttcttcaa
    12721 ctgcctgtac cgcctttttg tcacgcagac gaatttcaaa acggttgata atatcggtat
    12781 gtttcttttt cgttgcctgt tccttttgtt tttcatacag gcagaaatat tttgtgcttg
    12841 cctttgagcc gatataaagg gtacttgcta aattacggtt ttttccgctt aattttccac
    12901 cttgtacatt ttcatagttt ttacagcgac attccgcacc accatttctg tatttttcag
    12961 atagcacagg aatatccagc agaccgcaca tatcattgat tgccaagtca aaacgtcgga
    13021 taacaccgcc acagtcaaga cagcggttta agaatgaata ccagtccctg ttctgtgcct
    13081 gtaagacata ttccatgttg cgtgagcctg ccccttttaa ttctaaaaat acgcccatgt
    13141 gttcatcatc agaagccatg accttgattt ccccgtaagc ataatgctcc ttatagccat
    13201 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    13261 cttccaatgc gtcagtcgtt ggaaaacgga cagacagata atcaacgagc agataaaaag
    13321 gcttatcgca gatatagtaa ttcagacaac gcaggataat gttctgtgtt tccttgctgg
    13381 cgttagcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    13441 caagttctcc ttgtgaaaga ccgctggcaa gacgtttgtt cttcatttca agtgcaaatt
    13501 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaaccacc
    13561 tttcaaaaag tgtgatatgt actatcttct taaaacctct cattactact cacaggtttg
    13621 ggggtgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    13681 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    13741 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    13801 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    13861 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    13921 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    13981 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    14041 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    14101 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    14161 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    14221 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    14281 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    14341 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    14401 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    14461 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    14521 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    14581 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    14641 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    14701 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    14761 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    14821 aggcaacgca ggtatcacag tatgaattga caccgcataa ggaaagcaac tggaagattg
    14881 tagaataaat gaaaaaggct tgcattctgc atttttataa aattgcaagt ctttatattg
    14941 acaagcataa aatactaaga tatactttaa tcagaccaac ggtttgtatt ggaggtaagt
    15001 tatggcacga aataaatatc cagaagaaac agtaaaactc attttagatg tttctacaaa
    15061 aatgttttgt gaaaaaggat atgataacac atcattgcaa gatattatca atgaaacaaa
    15121 actttctaaa ggtgctattt atcatcattt cgattcaaag gaagatattt taaaagcaat
    15181 ttttcaaaga attggaaatg aaaatgcagt aatatttgct aaaattagag ataacgatag
    15241 tttaaacggt ttagaaaagt taagagaaat atttaagaca gcggtattca attctaacca
    15301 aagcgtttta ttgacagttt ctcctcaact attaaacaat cctcgttttc ttgctatgca
    15361 aatagaacaa atgtatgaaa ttgtagtacc tgaatttgtt gaacccgttt tagaacaagg
    15421 tatcaaggat aagtctttgg agattgaaaa ttcacatgaa attgcagaag ttatcatggt
    15481 attaagtaat gtatggctca acccattagt tgaaatgacg gataaagagg gtatggaaaa
    15541 acgttgcaat acattcaatg cccttttaaa tggaataggc atagacacat tattagacga
    15601 agaaacaatt tctgcattta tacagttttg taaataactg tatttgttat ccctatacga
    15661 tagactgctc tataaaagag cagtttattt aaaccattat ataaaccgac ggtctgtttg
    15721 attaaataaa aaggagagat tttatgcaag cagaaaaaaa actttttaat cgtgatttta
    15781 tacttgttgt tataggacag ataatttctt tatttggaaa cgctatccta cgatttgcat
    15841 taccgtacta tttgttaaaa gaaactggtt catcagcttt gtttggtatt gctacagcct
    15901 gctctttttt acctatggtt attttgtcgc tggtaggtgg tattattgct gacaggacaa
    15961 ataaaagaaa cattatggtc atactagatt ttgtaacagc aatcataatt ttagtatttt
    16021 atttgtcatt gggaaaaatt cctatcatac ctttgtttat tgttgctttg atgttacttt
    16081 atggtatttc tggtgcatat caaccagcgg tacaagcaag tattcctcta ttggtatcat
    16141 cagataaact taccgtcggt aatgctataa taaatcaagt aaatacttta gcaaacttta
    16201 tcggaccagc tataagcgga gtagtttttc aattttatgg tatcaatccc attttaattg
    16261 ttagtattat atgttttact ttttcagcca ttatggaaat ttttatccat attccacacc
    16321 aaaaaagacc tcgtgaagta ggtgtaattg cagttgctaa aaacgattta aaagatagtt
    16381 ataccttttt gaaaaaggag aaacccattt ttatatccgt aatctttttg gtttgcattt
    16441 tcaatttggt tttaagtgct gttttaattg ttggtctgcc tatcttaatt tctgaagttt
    16501 taaaaatgcc agatacacta tatggttttt cacaatcagc attatcatta ggtggtttat
    16561 gtggtggtat tttaacagct cttgttgctt caaagttaaa actaaaaaac ctttatacct
    16621 tgttacttat ttgttctgtt tctgttttac ttatgggaat ttcgctattt ttagaattgc
    16681 ctgcaatagt ttcctactgg gtagttacag tgatgtgctt tgtggctatg ggtgtatcta
    16741 ctatgtttgt tgttcaaatt tacactcttg ttcagacaca aacaccaccg caattagtag
    16801 gaaagataat ggctacgctg gtagcagttg ctatgtgcgg tcaacctatt ggacaaatgg
    16861 tttacggtat tttattcgat gtatttatac aagaaacatg ggttgttatg tttgtagctt
    16921 ctattgcttc aatcatgatt tcaatatatt caaaaagaat ttttcaaaag ttagagatat
    16981 aatacaaggg gttaaatagt atgttaggct aaaaacaata tataaaagaa aatttgaaat
    17041 ctgctaaaag gatagtgtta taaaagaaag agtaatattg cagtatttaa ggctcgcaca
    17101 aatatatgtt ttgtgtgggc ttttttctta ccatttttct gtatttctaa agggtataaa
    17161 cggtattccg tttatatatt tcagcattag cagaaagggg gtgagattat gaggacatct
    17221 tctttcgagc atatcgtcag attacagttc aattctttaa tgctgattgt cattaagaac
    17281 aaagtgaaaa gtcgaaacag acagcttgca agacattcta aacgtgaagt attgttttgt
    17341 gaaatagccg aaacgaaaca agttgaacgt ggaacgattg atacctgttt ttatgattgt
    17401 gtatcattta aagtcttgca ctttacaatt tatgtatctg atgaaacact tggtacagtt
    17461 ttacatagac tgtcagaaaa acagcgtagt gcgattttat tacgttattt tcaaggaatg
    17521 aacgacagag aaatttcaga actgtaccat gtatcacgtt ctgctatttg cagtagaaga
    17581 actagaggac taaggaagtt aaaaatgctt ttgagcgaaa ggaagtaaag aaatgagtaa
    17641 agattatggc tatccctctt ttgaattgat ttgcaaagcg tcaagcggag atgaaatggc
    17701 agttaaagag attttgaagt tttatgacgg ttatatttct aaattatgct tgcgtccttt
    17761 ttaccactct gaaagtggta aaatcattat gcaggttgat gaagaactaa aaggagaaat
    17821 tcatacagac atgatgaaag caatattgaa atttgaaatc ggagtgaagt aatcaactgt
    17881 ataaaaaaat cgggagatac acagtttcgt gctatctccc ttagtagttt tacggtcata
    17941 tcaaggctca ttcaatcgtg gtaaattcgt ggtaagatga gtgtttttct atacttcaaa
    18001 atgcttgaat gtatagtgtt tatgcggata attgaaaatt agatataaaa tttcaaataa
    18061 attactgata taaatttaca ataagaactc ttgtttctac ttttttttat gagaaaattt
    18121 agttttcaat ttattgaacc attttcggct tggatctcga cgttttttga tttgatgacc
    18181 cgtcacgtat tcttgaacaa ctgcattgat gattccgccc aaaatgatga tcgtagcagc
    18241 aaaattaagc caaagcatga gtacaataaa acttcctatg atctgataac cgctgatcct
    18301 cgttgcaaaa tacctagcat agatactaaa cacttgtgat aacagcatcc agccgacagt
    18361 agcgaagatc gtgcccggaa tgatcgtctt caacttcaac tgagcattag ggacaattaa
    18421 ataaatcaag aacatgatga caaataaagc taccaatgtg attggccact tcaaagtttg
    18481 gaactgatcg ataaagttca cgggtatttg tacgattggc tgaatagcat ccaaaatcag
    18541 ctgtcccaac ccaagaacga tagttacccc actaatagca atcatgaaca agacaatgac
    18601 aattagtgag aagaaacgga cgatcaaaaa attttttcta ttttctacgc catacgcttt
    18661 attcatcgca atttgcaaag cattgatact ttgacttgca gaccataaag ccgctaatgc
    18721 agaaacggat aacaagcctc ctgaactttg tgtcaaaagg tctttgattg caggaccgat
    18781 aaactggtaa acggtctcag gaatcacttc tcgtaaatag gtcagtacag tctcttgatc
    18841 gatgtggaga aacggtaaaa tattaccaaa agcaataatc aatggaaata aagacaagag
    18901 taaataataa gcaacgacga cagaggtcgt actcatttct gaatcagtga tgcgcttttg
    18961 agtcgtctca ataaagcgca tcagattttc attttgtttt attttgttcc aaactttcat
    19021 gtacctctcc cgtacctatt gactggatag atcaataagt gccttcttct ccttgtgatg
    19081 tcaaaattct tggaccatct ttagtaatag ccaatgtatg ttcatattgg caagaaagtc
    19141 cgccgtctct tgtatacgca gtccagccgt taggatccat tttcattttc caagttccgg
    19201 tattaaccat aggttcgatc gtgataacca tgccttcttt caaccgcaac ccttttccag
    19261 cttctccgta atgaggaact gctggttctt catgaatcgt aggaccaacg ccatgaccaa
    19321 taaaatcacg aacgatcgct agattttcgc cttctacata agtttggata gcatgaccga
    19381 tgtcaccaat ccgattccct acttgtgctt gctcaatacc gatgtacaaa gctttcttcg
    19441 ttacttccat caatcggtcg atctcaggtg tagattctcc aactacatag gcccaacaag
    19501 aatcagaaat gccgcctttc aaatcgatac acatatcgac ttttaccaaa tcaccgtttt
    19561 tcaaaggctt tttacgaggg aatccatgac aaatttcatc atttatactg atacaagtcg
    19621 catattcata cccttcaaag ccaatttgcg cagcaattcc gccatgactc tcgatatagt
    19681 ttctgacaaa tacctcaata tcccaacttg tgattcctgg ttttataaaa tcacgcagat
    19741 ttttatgtac atctgctaat aaagctccag attctgccat catttcaatt tcacgtgctg
    19801 attttaaagt aatcatattt tcgtctcctt tttacaaatc cctctcattt taacatagcg
    19861 gcatacattt agccaaatga cattccccag gtgtctctag gttttatttt cttttcaatg
    19921 atgttttggt ataattcata attgttaaat aattataaac gaggaggcac atcatggctt
    19981 tagcaaaaat cgtatatgct agcatgacag gaaacactga agaaatcgcc gatattgttg
    20041 cagaagcatt tgaaaatctg gatatcgaag tcgaaatcaa cgaatgtacc caagtagatg
    20101 cagatgaatt cgaggaggca gatatttgcg tagtagctac ttatacatac gatgatggcg
    20161 atctgcctga tgagatcgta gacttttatg aagacttgca agaattggat ctattgggaa
    20221 aaatttacgg tgtctgcgga tctggtgata ctttttacga tgaattctgc aaatcggtgg
    20281 atgatttcga tgctgctttt gcaaaaacag gcgcatcaaa aggagccgaa aatgttaaag
    20341 tggatctgaa tgctgaagaa gaagatatcg aaaatttaga agctttcgct aaagaactag
    20401 tcgcaaaaat ttaatatact tgcaataaaa gagccatcca gatttattca atctgtatgg
    20461 ctcttttatt cattgcattc atggaagagt tcttcgggag ttaaaaatgc atacgctgta
    20521 agaaaaacga acttttcctt attttgacac atctcttgtt acctactgat ttgtggaact
    20581 tttgagtata ttcaatcgat ttcgattggt tctttgaatt tcaagatagc atctatactg
    20641 tcttggatca tcttttcaat ctgttcatca gttggatgga tgatgcttga agacatcaaa
    20701 gctgcgatcc cagtagctat gatccatgtc ccattataca aagattcgat ctgctcttct
    20761 gaaagattca cataagctgg atcgcgtttc actgtattca caaagtaagt ataagagaaa
    20821 ttctgcatct cttttcctcc accatattct tctaagtaca gtgaacgata caacttcttc
    20881 tctcttcttg caaaatggat ataattaaaa gcaagatcga taatcgtatt ccctgtatgc
    20941 tcaacagtga acacttcata ttgtaaatga tcatagatct gctcaaaaag agcttgtctc
    21001 aagtcatcca tatttttaaa ctcaagataa atcggttgcg tggaacaatt cattttgttc
    21061 gcgatgtttc tcgcagtaaa tcgtgtaaat ccatcggtgg cagctacttc ataagcagct
    21121 gccaaaatct gttctctagt aatcgtcttt tttcttgcca tattttttaa tctccttaat
    21181 ttttttataa cgcttccacc attgattaat ttcattgtaa catgtttttt ttgatatgca
    21241 aaattagttt ttgattttct tcacaagctg aaatagcaag tagtaacata tatcatgaaa
    21301 cttaacctta aaaaaataac ctcctttttt tatttaaagc ctcattgttt gaatcataat
    21361 taaatggtat catgctaata gttaccagaa aaaatggtga ggaggaattt ttattatgtt
    21421 atctgatttt aatgaacttc taaaaaaata tgcacgctta attgcagaaa ctggcgtagc
    21481 cacagaaaaa ggacacacag ttgttttaca gatcagcgtg gaacaggcac cccttgctcg
    21541 attgatcaca caagaagctt ataaactagg agccgctgag gttatcgttc aatggactga
    21601 cgaccagatc caaagagaat ttcttttaca tgctgcaacg gatcgtattg agaatgtacc
    21661 acaatctaaa atcgatcaag cggatgaatg gcttgaaaaa ggagcaagca ggatcagcgt
    21721 tgtttctgcc gatcccgatg catttgctgg tgtagatagc catcgagtag cgtcttatca
    21781 atcagcgaat ggcaaagcac taatgaattt acgcaaggca actcaagcaa ataaagtcag
    21841 ttggactgtc gtggctgcgg ctggtaaaca atgggcagca aaagtcttcc cagaactgcc
    21901 agaagaaaaa caagttgagg cattatggga tcagattttc aaaacgactc gtgtctatga
    21961 agaagaccct gtcttagcat ggaagaagca tgatgaaaaa ttagcaaaaa aagcagaaga
    22021 attgaatcga gaacaatttt ctgcccttca ctatactgct cccggtacag atatcattat
    22081 cggattgcct aaaaaccatc tttgggaagg cgctggaagc tataatgctc gtggagaaaa
    22141 attcatggca aatatgccga cggaagaagt gttcacagca cctgacagtc atcgagtaga
    22201 tggttacatt tcaagtacaa agccactgag ttatgctggc accatcatct cgggtatgaa
    22261 atttactttt aaagatggaa aagttgttga tttttctgcc gaacaaggag aagatgtctt
    22321 agcaaaactg cttgatacag atgaaggtgc gcgtcgttta ggagaagtcg cacttgtacc
    22381 tgacccttca cctatttctc aatctggcat catctttttc aatacattat ttgatgaaaa
    22441 cgcatcgaat cacttagcac taggttctgc ttatgccttc agtgtgaaag gcggtacaga
    22501 aatgtccgat gaagaattag cagaagctgg attgaataga agtcaaaccc acgtagattt
    22561 catggtagga tctgacaaga tggatatcga cggaatccgt gaagatggct ctacagtacc
    22621 gattttccga aatggcgatt gggcttgagt atactttcgc acacatgcaa aaaataatgg
    22681 ctctttgtca ataaggactg atgagttgtg caaaa
//
LOCUS       DAZUJO010000249        11482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.083, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000249 DAZUJO010000000
VERSION     DAZUJO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.083"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(371..607)
                     /locus_tag="ACG8OL_003079"
     CDS             complement(371..607)
                     /locus_tag="ACG8OL_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818956.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(790..1161)
                     /locus_tag="ACG8OL_003080"
     CDS             complement(790..1161)
                     /locus_tag="ACG8OL_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4818957.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1158..2486)
                     /locus_tag="ACG8OL_003081"
     CDS             complement(1158..2486)
                     /locus_tag="ACG8OL_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4818958.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACG8OL_003082"
     CDS             complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACG8OL_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4818959.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(3034..3405)
                     /locus_tag="ACG8OL_003083"
     CDS             complement(3034..3405)
                     /locus_tag="ACG8OL_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4818960.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            3550..3735
                     /locus_tag="ACG8OL_003084"
     CDS             3550..3735
                     /locus_tag="ACG8OL_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4818961.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3893..4060
                     /locus_tag="ACG8OL_003085"
     CDS             3893..4060
                     /locus_tag="ACG8OL_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818962.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4678..5337
                     /gene="vanR-B"
                     /locus_tag="ACG8OL_003086"
     CDS             4678..5337
                     /gene="vanR-B"
                     /locus_tag="ACG8OL_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS4818963.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5340..6683
                     /gene="vanS-B"
                     /locus_tag="ACG8OL_003087"
     CDS             5340..6683
                     /gene="vanS-B"
                     /locus_tag="ACG8OL_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4818964.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            6854..7660
                     /gene="vanY"
                     /locus_tag="ACG8OL_003088"
     CDS             6854..7660
                     /gene="vanY"
                     /locus_tag="ACG8OL_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS4818965.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            7678..8505
                     /gene="vanW-B"
                     /locus_tag="ACG8OL_003089"
     CDS             7678..8505
                     /gene="vanW-B"
                     /locus_tag="ACG8OL_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS4818966.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            8502..9473
                     /gene="vanH"
                     /locus_tag="ACG8OL_003090"
     CDS             8502..9473
                     /gene="vanH"
                     /locus_tag="ACG8OL_003090"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH"
                     /protein_id="HFS4818967.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGARVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            9466..10494
                     /gene="vanB"
                     /locus_tag="ACG8OL_003091"
     CDS             9466..10494
                     /gene="vanB"
                     /locus_tag="ACG8OL_003091"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4818968.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            10500..11108
                     /locus_tag="ACG8OL_003092"
     CDS             10500..11108
                     /locus_tag="ACG8OL_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4818969.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(11174..11347)
                     /locus_tag="ACG8OL_003093"
     CDS             complement(11174..11347)
                     /locus_tag="ACG8OL_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818970.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         3001 a         2612 c         2943 g         2926 t
ORIGIN      
        1 ccctttccaa gctggaacgc atgacggatg ccgactatga cggccagaag ttccacttca
       61 cagacgaata acgaacagca gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc
      121 gtcttttctt tttgcctatt cctacgcatt tagaacccgg tattgtcggg gataggatga
      181 agtattcgct tgcccctgca aacatgcggg agaagccttg cagcgggcgg ttttcaatcc
      241 cttaaatgcg taggttgcag ctataattag acccctgaaa cagatatagc cgcgcagcgg
      301 gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc
      361 ctcttgccgt ctatctctgt tccagcggga agctcggcaa tgcttccagc agtttcaggg
      421 aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt tttgccgttt gcctgtctga
      481 tctccaccgt ggaaagctcg ttgatgtagt cagcataatg ggccaacact ttttccgtgg
      541 cccacctctc accggcaacg gcggctttga tcgtttcata gtccagcagc ttttcttgtc
      601 cgtccatcct gatacccccg tcagactgat tttttctatg ataccatatt cggaagcatg
      661 gaaccagaga aaacggcgct tttctgctgg taaattctac gcatttagaa cagcacttcg
      721 gcggggatag gataaaacac ccacataccc cgctgaccat gccggaaaag ccttgaaata
      781 taagggcttt tacagctcta agtgcgtagg tttccagtat cttttccgct ggcgctcggc
      841 ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag tatttggccc ggttagactt
      901 cggcgtgaac gtggccccgc actcacagca gcgtttcact tcgtcccggt ctttggtgat
      961 ctcggcatac agggccgcat ccagcgggag gacagcggcc ttaaaccagc ggcacagaag
     1021 cgaataggaa atgctctgta cgcaaataca ttcttcgccg tcgtccagca acaggcagtt
     1081 gccgtcacaa tagttgcagc actcatgcgt cagcttccgg gccttgcgat attgagggta
     1141 attcattcgt ggaatactca tagctcttgc gtccattcct ttcccggcgt catacgcaaa
     1201 atgccgtcca cattctgctt gatgatgccg tattcctgca tctgcttttt cgcttcgccg
     1261 tacttctcat acagccgttc cttttcctcg gccaatcgct tgtactcggc tttcagggcg
     1321 tacagatcgg ggagcttttt cacaccctgt ccctttaggg cctttgccgc agcttcatac
     1381 aaaatcaacg tgctttcatg ctcacgccgg aattttgcct tatccgctgc attgcggtat
     1441 tccatagcca cgggccagag ctgcttgtag gtagaaatat tctttatcag caccgccata
     1501 tccgcaagcc ggtgttccat agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg
     1561 atctcggcca ctttgctttc caaatccggg taggtgtcta tcccatgttc agtcaggaaa
     1621 ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg cccacctctc atagcctgcc
     1681 gactgctggg ccttaatgtt gttttccaga tcaatccgca aagaaatccc cttgcgctct
     1741 ttgggcgctc tggtgataac gcggcctttg atacgctctt tgatggcttc ctcggtgtag
     1801 gcttccccca atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata
     1861 aactttcccg gcttgatctc atagccttgc gcctgcatga gccgcaggaa atcatcaaaa
     1921 tcttttgcct gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc
     1981 cctttccgct gggcgtccca ctcggcatag ctcttgccct tactctggcc gggcttcacc
     2041 acggacaatc cgttttcctt gcacagcctg tcgctggtgc gccggatata ggcatagctc
     2101 tgtttgttgg aaatgtactt gcgctgattt accatatcca cggcacagaa aataatgtga
     2161 ttgtggacgt ggcccttatc aatatgggta gtcagcacat agggatattt cccttgcagc
     2221 acttcctcgg caagctgctt cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg
     2281 gcaaaggatt gtatcaggtg gtggcccaga ttattgccct tttggtatgc ctgcgccagc
     2341 agcatttcaa attcaatgtc cgctgtttca taagagcagg caaaggaggc caccagcagc
     2401 ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg cttttttcag cgttgacttg
     2461 ataggatgta tctttgtaac tgccatatct gcgccagcgc cccctttata tcctcgatgt
     2521 cctgtgcgta caccggcccc ataccgttca gcctctttgc aatctgattg acgttgacgc
     2581 cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat
     2641 agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca
     2701 ttttctgttc aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc acgatttttc
     2761 gctttctgtt tgccatgctt caccgtcctt tccattcagg ggtttgggga tttccccaac
     2821 aagcaaaacg gacgcggccc ggcagggacg gggcaggccg tttttccggg agtggctaca
     2881 ctccctatgc ttgctacgtt actgtttctc cactaacaag acgtttccaa agccccaaac
     2941 tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc
     3001 agctcccgct aaaagtaaaa cagaggatgc cagttacaca tcctccgttt cattcttctt
     3061 cggcttttca attagtatgg cgctgtcaat cgcgccctcg atgatcggca ggtattcttc
     3121 ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct cccgcatgat
     3181 ctccgggtta aagtagcgtt ccacaggaag cccgcaggct ttaataagct gtatgacaac
     3241 cggcagactg ggaatggccc cgtcgttttc gatgttggca agatagcgcc agtcaatatt
     3301 tattttctct gccattgctt tgcgcgaaac gcgctttgct tgccgggccg ctttcacatc
     3361 cgcgccgaaa gtttcaaagc cgggacaatc ttcaatattc gccatatcgc acctcccact
     3421 tacattgtat atttcatact ctgcccgtgg aatgttggta tatgcacctt tcttaaagtt
     3481 tatatttcac attttgtttc ttttattctt cgagcaaaag aattataatg taccctgtgg
     3541 aggtgtacta tggactacat gacgttgaaa gaggcaggcg agaaatgggg tgtgccccca
     3601 cgatggataa actactattg tgctgcggga cgcatccccg gcgctgtaaa gatggcgacg
     3661 atctggctca ttcccaaaac tgcggaaaag ccgattgaca ggcgtacaaa gaaaggaaag
     3721 gcggttactg aatagtataa tgaaaccggc gcattttgga tatggttttg aagagagaga
     3781 aagcatggta ttttgtcacg gaaaaactat aagatgatag atggcaggct gctccagacc
     3841 gataaaaagt attctcatct gaaacagaaa gaaaagattt acacatggat gtatgaagaa
     3901 gcgaaacttt atcatgacag aaatggaaaa tgcccgaaac aaagacgagg aagctgctgc
     3961 tgtatcagcc gtatatggta gaattgaaaa ggccggcatc tggattcttt atggtgaagt
     4021 ctccagacat tggataactg ctggtctaat gcgcctttga gtatggggtg attgatttta
     4081 ataccgggga ggtacggctt tttgaggaaa atattctggg atttacattt ggcgattatt
     4141 acatttcctg taactgcaag gacacggatg atgaagatat gaggaatata gacttgctga
     4201 ttttctactg tcttgaacgg tattaggact gaatctgctg cgcggagtgc ggcaagagcg
     4261 accggaggga gtacacacag gccctgcctg tgtgaaaagc cataccgcaa ggtgcattcg
     4321 caaaaaagag gccgcgcatt ttgctgaggc ttggggtgtc cccaacaagc aaaacgagaa
     4381 cgccgcaggc gggcaagtgg tggatcagaa tatccgtaaa ggatattctg atccattgct
     4441 tgccagtatt atcgggatgt tttgacaacc gctgtaatcg aaacatccca aacattaaga
     4501 aggtgttaat tattattatc ttgatttaat tcgtttaaac caataatata gatacataca
     4561 ccatgaaaag aagcgagtat cccaatgacg gatgattaaa atccgaaaag aaatcgcttt
     4621 gaaactacag ggaaactaca gactgttatg ttatcttctt aaaatggagg gatttttatg
     4681 tcgatacgaa ttctacttgt cgaggatgat gatcatatct gtaatacagt aagggcgttt
     4741 ctggctgagg caggatatca ggtggatgcc tgcacagatg gaaatgaggc atacaccaag
     4801 ttttacgaaa acacttatca actggttatt cttgatatta tgctgcccgg tatgaacggg
     4861 catgaacttt tgcgtgaatt tcgtgcgaaa aatgatactc ccattctgat gatgacagcc
     4921 ctgtcggatg acgaaaacca aatccgggcg tttgatgcag aggcagacga ctatgtaaca
     4981 aagccattca agatgcagat tttactaaag cgggtggaag ccctgctgcg gcgcagcggt
     5041 gcgctggcaa aggaaatccg tgtcggcagg ctgacacttc tgccggagga tttcacggta
     5101 ctttgtgacg gtacagagct gcccctgaca cgaaaagaat ttgaaatcct tttgctgctg
     5161 gtgcagaaca aaggcagaac cttaacgcat gaaatcattt tgtcacgtat atggggatat
     5221 gactttgagg gtgatggcag cacagtccac actcatatca aaaatttgcg ggccaagctg
     5281 ccggaaaata tcatcaaaac cattcgcggt gtaggatacc gattggagga atcataataa
     5341 tggaaagaaa agggattttc attaaggttt tttcctatac gatcattgcc ctgctactgc
     5401 ttgtcggtgt aacagcaaca ctgtttgcac agcaatttgt gtcttatttc agagtgatgg
     5461 aattacagca aacagtaaaa tcctatcagc cattggtgga actgattcag aatagcgata
     5521 ggcttgatat tcaagaggtg gcagggctgt ttcactacaa taaccaatcc tttgagtttt
     5581 atattgaaga taaagaggga agcgtactct atgccacacc aaatgccaat acatcaaata
     5641 gttttagacc cgactttctt tatgtggtac atagagatga taatatttcg attgttgctc
     5701 aaagcaaggc aggtgtggga ttgctttatc aagggctgac aattcgggga attgttatga
     5761 ttgcgataat ggttgtattc agccttttat gcgcgtatat ctttgcgcgg caaatgacaa
     5821 cgccgatcaa agccttagcg gacagtgcga ataaaatggc aaacctgaaa gatgtaccgc
     5881 cgccgctgga gcgaaaggat gagcttggcg cactggctca tgatatgcat tccatgtatg
     5941 tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa catgagttgg
     6001 aggaaacaca gcgatatttc tttgcggcag cttctcatga gctaaaaacg cccatcgcgg
     6061 ctacaagcgt tctgttggag ggaatgcttg aaaatatcgg tgactacaaa gatcattcta
     6121 agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt tccgaaatac
     6181 tggagcttgt cagcctgaat gatgggagaa tcgtacccat agctgaaccg ttggacatag
     6241 ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca aacaaccagc
     6301 ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc cgatccgagg ctgctccaaa
     6361 aggcactatc caatgtcata ttgaatgcag ttcagaacac gccgcaggga ggcgaggtac
     6421 ggatatggag tgagcctggt gctgaaaaat gccgcctttt tgttttgaac atgggcgttc
     6481 acattgatga tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt gatcaggcgc
     6541 aaagcagaaa aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg
     6601 ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt
     6661 taccgctcac atcaacattg taaatattta aaacttaaat gattttgacc gacaggtata
     6721 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
     6781 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
     6841 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
     6901 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
     6961 ctgcacgctg tttttggggc ggagactggc ttccgtattg gaggcaacac agataccgcc
     7021 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
     7081 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
     7141 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
     7201 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
     7261 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
     7321 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
     7381 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
     7441 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
     7501 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
     7561 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
     7621 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     7681 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     7741 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     7801 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     7861 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     7921 ttaaacggct tattgataag accgggggaa cccttttctt tctggtggct ggtacgccat
     7981 gcggacaaag atacccccta taaagacggc cttacggtga ccaatggtaa actcaccacc
     8041 atgtcgggcg gcggtatgtg ccagatgagc aatttactat tttggatgtt cctgcatacg
     8101 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     8161 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     8221 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     8281 caggtgtccg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     8341 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     8401 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     8461 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     8521 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     8581 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     8641 ctggcaatca atgcgttagc gtaggccata agtccgaggt ttccgaggcg acaattcttg
     8701 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     8761 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     8821 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     8881 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     8941 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     9001 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     9061 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     9121 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     9181 taatcaacac cgggcgcgga gcgcgtgtcg ataccggggc gctggtggag gcgttggaaa
     9241 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     9301 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     9361 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     9421 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     9481 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa atccgcaata
     9541 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     9601 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     9661 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     9721 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     9781 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     9841 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     9901 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     9961 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
    10021 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
    10081 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
    10141 attgtcggcg aagtggatca aatccggctg agccacggta ccttccgcat ccatcaggaa
    10201 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
    10261 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
    10321 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
    10381 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
    10441 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
    10501 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
    10561 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
    10621 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
    10681 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
    10741 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
    10801 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
    10861 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
    10921 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
    10981 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
    11041 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
    11101 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
    11161 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
    11221 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
    11281 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
    11341 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
    11401 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa
    11461 gttgaactat ttaatggact gt
//
LOCUS       DAZUJO010000250        11482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.084, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000250 DAZUJO010000000
VERSION     DAZUJO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.084"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(371..607)
                     /locus_tag="ACG8OL_003094"
     CDS             complement(371..607)
                     /locus_tag="ACG8OL_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818971.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(790..1161)
                     /locus_tag="ACG8OL_003095"
     CDS             complement(790..1161)
                     /locus_tag="ACG8OL_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFS4818972.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1158..2486)
                     /locus_tag="ACG8OL_003096"
     CDS             complement(1158..2486)
                     /locus_tag="ACG8OL_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS4818973.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACG8OL_003097"
     CDS             complement(2447..2776)
                     /gene="mobC"
                     /locus_tag="ACG8OL_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFS4818974.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(3034..3405)
                     /locus_tag="ACG8OL_003098"
     CDS             complement(3034..3405)
                     /locus_tag="ACG8OL_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS4818975.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            3550..3735
                     /locus_tag="ACG8OL_003099"
     CDS             3550..3735
                     /locus_tag="ACG8OL_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS4818976.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3893..4060
                     /locus_tag="ACG8OL_003100"
     CDS             3893..4060
                     /locus_tag="ACG8OL_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818977.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4678..5337
                     /gene="vanR-B"
                     /locus_tag="ACG8OL_003101"
     CDS             4678..5337
                     /gene="vanR-B"
                     /locus_tag="ACG8OL_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFS4818978.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5340..6683
                     /gene="vanS-B"
                     /locus_tag="ACG8OL_003102"
     CDS             5340..6683
                     /gene="vanS-B"
                     /locus_tag="ACG8OL_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFS4818979.1"
                     /translation="MERKGIFIKVFSYTIIALLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQAQSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            6854..7660
                     /gene="vanY"
                     /locus_tag="ACG8OL_003103"
     CDS             6854..7660
                     /gene="vanY"
                     /locus_tag="ACG8OL_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS4818980.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            7678..8505
                     /gene="vanW-B"
                     /locus_tag="ACG8OL_003104"
     CDS             7678..8505
                     /gene="vanW-B"
                     /locus_tag="ACG8OL_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HFS4818981.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGEPFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            8502..9473
                     /locus_tag="ACG8OL_003105"
     CDS             8502..9473
                     /locus_tag="ACG8OL_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFS4818982.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            9466..10494
                     /gene="vanB"
                     /locus_tag="ACG8OL_003106"
     CDS             9466..10494
                     /gene="vanB"
                     /locus_tag="ACG8OL_003106"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFS4818983.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGTFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            10500..11108
                     /locus_tag="ACG8OL_003107"
     CDS             10500..11108
                     /locus_tag="ACG8OL_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFS4818984.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(11174..11347)
                     /locus_tag="ACG8OL_003108"
     CDS             complement(11174..11347)
                     /locus_tag="ACG8OL_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818985.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         3001 a         2612 c         2942 g         2927 t
ORIGIN      
        1 ccctttccaa gctggaacgc atgacggatg ccgactatga cggccagaag ttccacttca
       61 cagacgaata acgaacagca gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc
      121 gtcttttctt tttgcctatt cctacgcatt tagaacccgg tattgtcggg gataggatga
      181 agtattcgct tgcccctgca aacatgcggg agaagccttg cagcgggcgg ttttcaatcc
      241 cttaaatgcg taggttgcag ctataattag acccctgaaa cagatatagc cgcgcagcgg
      301 gtgtatctgt ttcaggggtg tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc
      361 ctcttgccgt ctatctctgt tccagcggga agctcggcaa tgcttccagc agtttcaggg
      421 aaatgcgctg ccgtatatct tcgtcaatga ctttcttctt tttgccgttt gcctgtctga
      481 tctccaccgt ggaaagctcg ttgatgtagt cagcataatg ggccaacact ttttccgtgg
      541 cccacctctc accggcaacg gcggctttga tcgtttcata gtccagcagc ttttcttgtc
      601 cgtccatcct gatacccccg tcagactgat tttttctatg ataccatatt cggaagcatg
      661 gaaccagaga aaacggcgct tttctgctgg taaattctac gcatttagaa cagcacttcg
      721 gcggggatag gataaaacac ccacataccc cgctgaccat gccggaaaag ccttgaaata
      781 taagggcttt tacagctcta agtgcgtagg tttccagtat cttttccgct ggcgctcggc
      841 ctctttgcgg cggcgcatcc gtttggagca ggccgcgcag tatttggccc ggttagactt
      901 cggcgtgaac gtggccccgc actcacagca gcgtttcact tcgtcccggt ctttggtgat
      961 ctcggcatac agggccgcat ccagcgggag gacagcggcc ttaaaccagc ggcacagaag
     1021 cgaataggaa atgctctgta cgcaaataca ttcttcgccg tcgtccagca acaggcagtt
     1081 gccgtcacaa tagttgcagc actcatgcgt cagcttccgg gccttgcgat attgagggta
     1141 attcattcgt ggaatactca tagctcttgc gtccattcct ttcccggcgt catacgcaaa
     1201 atgccgtcca cattctgctt gatgatgccg tattcctgca tctgcttttt cgcttcgccg
     1261 tacttctcat acagccgttc cttttcctcg gccaatcgct tgtactcggc tttcagggcg
     1321 tacagatcgg ggagcttttt cacaccctgt ccctttaggg cctttgccgc agcttcatac
     1381 aaaatcaacg tgctttcatg ctcacgccgg aattttgcct tatccgctgc attgcggtat
     1441 tccatagcca cgggccagag ctgcttgtag gtagaaatat tctttatcag caccgccata
     1501 tccgcaagcc ggtgttccat agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg
     1561 atctcggcca ctttgctttc caaatccggg taggtgtcta tcccatgttc agtcaggaaa
     1621 ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg cccacctctc atagcctgcc
     1681 gactgctggg ccttaatgtt gttttccaga tcaatccgca aagaaatccc cttgcgctct
     1741 ttgggcgctc tggtgataac gcggcctttg atacgctctt tgatggcttc ctcggtgtag
     1801 gcttccccca atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata
     1861 aactttcccg gcttgatctc atagccttgc gcctgcatga gccgcaggaa atcatcaaaa
     1921 tcttttgcct gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc
     1981 cctttccgct gggcgtccca ctcggcatag ctcttgccct tactctggcc gggcttcacc
     2041 acggacaatc cgttttcctt gcacagcctg tcgctggtgc gccggatata ggcatagctc
     2101 tgtttgttgg aaatgtactt gcgctgattt accatatcca cggcacagaa aataatgtga
     2161 ttgtggacgt ggcccttatc aatatgggta gtcagcacat agggatattt cccttgcagc
     2221 acttcctcgg caagctgctt cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg
     2281 gcaaaggatt gtatcaggtg gtggcccaga ttattgccct tttggtatgc ctgcgccagc
     2341 agcatttcaa attcaatgtc cgctgtttca taagagcagg caaaggaggc caccagcagc
     2401 ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg cttttttcag cgttgacttg
     2461 ataggatgta tctttgtaac tgccatatct gcgccagcgc cccctttata tcctcgatgt
     2521 cctgtgcgta caccggcccc ataccgttca gcctctttgc aatctgattg acgttgacgc
     2581 cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat
     2641 agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca
     2701 ttttctgttc aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc acgatttttc
     2761 gctttctgtt tgccatgctt caccgtcctt tccattcagg ggtttgggga tttccccaac
     2821 aagcaaaacg gacgcggccc ggcagggacg gggcaggccg tttttccggg agtggctaca
     2881 ctccctatgc ttgctacgtt actgtttctc cactaacaag acgtttccaa agccccaaac
     2941 tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc
     3001 agctcccgct aaaagtaaaa cagaggatgc cagttacaca tcctccgttt cattcttctt
     3061 cggcttttca attagtatgg cgctgtcaat cgcgccctcg atgatcggca ggtattcttc
     3121 ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct cccgcatgat
     3181 ctccgggtta aagtagcgtt ccacaggaag cccgcaggct ttaataagct gtatgacaac
     3241 cggcagactg ggaatggccc cgtcgttttc gatgttggca agatagcgcc agtcaatatt
     3301 tattttctct gccattgctt tgcgcgaaac gcgctttgct tgccgggccg ctttcacatc
     3361 cgcgccgaaa gtttcaaagc cgggacaatc ttcaatattc gccatatcgc acctcccact
     3421 tacattgtat atttcatact ctgcccgtgg aatgttggta tatgcacctt tcttaaagtt
     3481 tatatttcac attttgtttc ttttattctt cgagcaaaag aattataatg taccctgtgg
     3541 aggtgtacta tggactacat gacgttgaaa gaggcaggcg agaaatgggg tgtgccccca
     3601 cgatggataa actactattg tgctgcggga cgcatccccg gcgctgtaaa gatggcgacg
     3661 atctggctca ttcccaaaac tgcggaaaag ccgattgaca ggcgtacaaa gaaaggaaag
     3721 gcggttactg aatagtataa tgaaaccggc gcattttgga tatggttttg aagagagaga
     3781 aagcatggta ttttgtcacg gaaaaactat aagatgatag atggcaggct gctccagacc
     3841 gataaaaagt attctcatct gaaacagaaa gaaaagattt acacatggat gtatgaagaa
     3901 gcgaaacttt atcatgacag aaatggaaaa tgcccgaaac aaagacgagg aagctgctgc
     3961 tgtatcagcc gtatatggta gaattgaaaa ggccggcatc tggattcttt atggtgaagt
     4021 ctccagacat tggataactg ctggtctaat gcgcctttga gtatggggtg attgatttta
     4081 ataccgggga ggtacggctt tttgaggaaa atattctggg atttacattt ggcgattatt
     4141 acatttcctg taactgcaag gacacggatg atgaagatat gaggaatata gacttgctga
     4201 ttttctactg tcttgaacgg tattaggact gaatctgctg cgcggagtgc ggcaagagcg
     4261 accggaggga gtacacacag gccctgcctg tgtgaaaagc cataccgcaa ggtgcattcg
     4321 caaaaaagag gccgcgcatt ttgctgaggc ttggggtgtc cccaacaagc aaaacgagaa
     4381 cgccgcaggc gggcaagtgg tggatcagaa tatccgtaaa ggatattctg atccattgct
     4441 tgccagtatt atcgggatgt tttgacaacc gctgtaatcg aaacatccca aacattaaga
     4501 aggtgttaat tattattatc ttgatttaat tcgtttaaac caataatata gatacataca
     4561 ccatgaaaag aagcgagtat cccaatgacg gatgattaaa atccgaaaag aaatcgcttt
     4621 gaaactacag ggaaactaca gactgttatg ttatcttctt aaaatggagg gatttttatg
     4681 tcgatacgaa ttctacttgt cgaggatgat gatcatatct gtaatacagt aagggcgttt
     4741 ctggctgagg caggatatca ggtggatgcc tgcacagatg gaaatgaggc atacaccaag
     4801 ttttacgaaa acacttatca actggttatt cttgatatta tgctgcccgg tatgaacggg
     4861 catgaacttt tgcgtgaatt tcgtgcgaaa aatgatactc ccattctgat gatgacagcc
     4921 ctgtcggatg acgaaaacca aatccgggcg tttgatgcag aggcagacga ctatgtaaca
     4981 aagccattca agatgcagat tttactaaag cgggtggaag ccctgctgcg gcgcagcggt
     5041 gcgctggcaa aggaaatccg tgtcggcagg ctgacacttc tgccggagga tttcacggta
     5101 ctttgtgacg gtacagagct gcccctgaca cgaaaagaat ttgaaatcct tttgctgctg
     5161 gtgcagaaca aaggcagaac cttaacgcat gaaatcattt tgtcacgtat atggggatat
     5221 gactttgagg gtgatggcag cacagtccac actcatatca aaaatttgcg ggccaagctg
     5281 ccggaaaata tcatcaaaac cattcgcggt gtaggatacc gattggagga atcataataa
     5341 tggaaagaaa agggattttc attaaggttt tttcctatac gatcattgcc ctgctactgc
     5401 ttgtcggtgt aacagcaaca ctgtttgcac agcaatttgt gtcttatttc agagtgatgg
     5461 aattacagca aacagtaaaa tcctatcagc cattggtgga actgattcag aatagcgata
     5521 ggcttgatat tcaagaggtg gcagggctgt ttcactacaa taaccaatcc tttgagtttt
     5581 atattgaaga taaagaggga agcgtactct atgccacacc aaatgccaat acatcaaata
     5641 gttttagacc cgactttctt tatgtggtac atagagatga taatatttcg attgttgctc
     5701 aaagcaaggc aggtgtggga ttgctttatc aagggctgac aattcgggga attgttatga
     5761 ttgcgataat ggttgtattc agccttttat gcgcgtatat ctttgcgcgg caaatgacaa
     5821 cgccgatcaa agccttagcg gacagtgcga ataaaatggc aaacctgaaa gatgtaccgc
     5881 cgccgctgga gcgaaaggat gagcttggcg cactggctca tgatatgcat tccatgtatg
     5941 tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa catgagttgg
     6001 aggaaacaca gcgatatttc tttgcggcag cttctcatga gctaaaaacg cccatcgcgg
     6061 ctacaagcgt tctgttggag ggaatgcttg aaaatatcgg tgactacaaa gatcattcta
     6121 agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt tccgaaatac
     6181 tggagcttgt cagcctgaat gatgggagaa tcgtacccat agctgaaccg ttggacatag
     6241 ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca aacaaccagc
     6301 ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc cgatccgagg ctgctccaaa
     6361 aggcactatc caatgtcata ttgaatgcag ttcagaacac gccgcaggga ggcgaggtac
     6421 ggatatggag tgagcctggt gctgaaaaat gccgcctttt tgttttgaac atgggcgttc
     6481 acattgatga tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt gatcaggcgc
     6541 aaagcagaaa aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg
     6601 ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt
     6661 taccgctcac atcaacattg taaatattta aaacttaaat gattttgacc gacaggtata
     6721 accctgccgg tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac
     6781 agatgcgctt gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga
     6841 ggcattcgat caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca
     6901 tatgaaacaa tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt
     6961 ctgcacgctg tttttggggc ggagactggc ttccgtattg gaggcaacac agataccgcc
     7021 catccctgca actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc
     7081 tcttgccacc gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag
     7141 gcagaacccc atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg
     7201 gatagatatt cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg
     7261 agtttacccg attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga
     7321 tgaaaaaatt gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc
     7381 agaaacttgg gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat
     7441 caatgcggac ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag
     7501 ctatcgtttt ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag
     7561 caacgagccg tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca
     7621 aggggtttgc cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg
     7681 gacagaaaaa gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga
     7741 aaaatgtgct tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg
     7801 gaaaaatcgc tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc
     7861 ggatttgaca tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc
     7921 ttaaacggct tattgataag accgggggaa cccttttctt tctggtggct ggtacgccat
     7981 gcggacaaag atacccccta taaagacggc cttacggtga ccaatggtaa actcaccacc
     8041 atgtcgggcg gcggtatgtg ccagatgagc aatttactat tttggatgtt cctgcatacg
     8101 ccattgacaa ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt
     8161 gacgagatta aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga
     8221 aacgataccg actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt
     8281 caggtgtccg ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag
     8341 tatgtccgtg aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc
     8401 ttaggtaccg gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt
     8461 tatcccctcc cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca
     8521 ttactgtttt tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt
     8581 ttcatattat ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg
     8641 ctggcaatca atgcgttagc gtaggccata agtccgaggt ttccgaggcg acaattcttg
     8701 cgctgagaaa ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg
     8761 atacgactgc cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca
     8821 gcgttgcgga ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca
     8881 tgcacgccgt ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg
     8941 atatgactgt gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc
     9001 ggggatttgg atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg
     9061 tccagcttga tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg
     9121 cggatacccg ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt
     9181 taatcaacac cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa
     9241 gcggaaagct gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata
     9301 ccgattgctc gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga
     9361 atgtgatcat cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag
     9421 aaaaaacaat caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa
     9481 gtcgcaatca tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa atccgcaata
     9541 gaaattgctg cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa
     9601 aacggtgtat ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc
     9661 gccatactct ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac
     9721 gaaacacggc gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt
     9781 gcgatacagg ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc
     9841 tccgcagctt gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc
     9901 gttcccgaat ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac
     9961 cctgtctttg tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt
    10021 acggaagaac ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt
    10081 gagcaagcga tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg
    10141 attgtcggcg aagtggatca aatccggctg agccacggta ccttccgcat ccatcaggaa
    10201 aacgagccgg aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc
    10261 gaggaacgaa atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga
    10321 gggcttgccc gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc
    10381 aataccctgc ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga
    10441 atcacgcttc ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta
    10501 tggaaaatgg ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt
    10561 acgccacatg ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca
    10621 tcggcacaaa ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg
    10681 gctatggctt gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc
    10741 gttgggcagc gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc
    10801 gagccgagtt gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg
    10861 caattgatct tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact
    10921 tcgattttat ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac
    10981 aaaatcgaag atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg
    11041 aatggtggca ctataagttg attgatgagc cataccccga tacctatttt aattttgccg
    11101 tttcataatg aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt
    11161 catggacaat cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat
    11221 agtattcaat ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca
    11281 cgcccatttt gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg
    11341 gcagcaaaag gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg
    11401 ctctaatcca cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa
    11461 gttgaactat ttaatggact gt
//
LOCUS       DAZUJO010000251         9921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.097, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000251 DAZUJO010000000
VERSION     DAZUJO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.097"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            187..357
                     /locus_tag="ACG8OL_003109"
     CDS             187..357
                     /locus_tag="ACG8OL_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4818986.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            371..973
                     /locus_tag="ACG8OL_003110"
     CDS             371..973
                     /locus_tag="ACG8OL_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818987.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            989..1666
                     /locus_tag="ACG8OL_003111"
                     /pseudo
     CDS             989..1666
                     /locus_tag="ACG8OL_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1977..2474
                     /locus_tag="ACG8OL_003112"
     CDS             1977..2474
                     /locus_tag="ACG8OL_003112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFS4818988.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2475..2891
                     /locus_tag="ACG8OL_003113"
     CDS             2475..2891
                     /locus_tag="ACG8OL_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFS4818989.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            2911..4455
                     /locus_tag="ACG8OL_003114"
     CDS             2911..4455
                     /locus_tag="ACG8OL_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS4818990.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4598..4822
                     /locus_tag="ACG8OL_003115"
     CDS             4598..4822
                     /locus_tag="ACG8OL_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS4818991.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            4837..5706
                     /locus_tag="ACG8OL_003116"
     CDS             4837..5706
                     /locus_tag="ACG8OL_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS4818992.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5687..6421
                     /locus_tag="ACG8OL_003117"
     CDS             5687..6421
                     /locus_tag="ACG8OL_003117"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS4818993.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6454..7362
                     /locus_tag="ACG8OL_003118"
     CDS             6454..7362
                     /locus_tag="ACG8OL_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS4818994.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7359..7901
                     /gene="sat4"
                     /locus_tag="ACG8OL_003119"
     CDS             7359..7901
                     /gene="sat4"
                     /locus_tag="ACG8OL_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFS4818995.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            7994..8788
                     /locus_tag="ACG8OL_003120"
                     /note="aph(3')-IIIa"
     CDS             7994..8788
                     /locus_tag="ACG8OL_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS4818996.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9064..9195
                     /locus_tag="ACG8OL_003121"
                     /pseudo
     CDS             9064..9195
                     /locus_tag="ACG8OL_003121"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9271..9764
                     /locus_tag="ACG8OL_003122"
                     /pseudo
     CDS             9271..9764
                     /locus_tag="ACG8OL_003122"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3494 a         1556 c         2169 g         2702 t
ORIGIN      
        1 ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc gaagcgaaca
       61 cttgatcttt taagttgcta tctttttaag gtcaatagag tatacttatt tgtcctaata
      121 tggttttagc agtataattg acttggtgaa taggtcattt aagttgggca taataggagg
      181 agtaaaatga aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt ggctattttt
      241 aatgagcaac aatatgcgca agattttatc gcttacgaaa agacaatttc tgataagcaa
      301 tttgaaattg aaaaagtaga tatagctgat tggttattgc aaccgagaga attttagagg
      361 ttggttgaaa atggctaaaa ttggttatgc acgtgtcagt agcaaagaac agaacttaga
      421 tagacaactg aaagcgttag agggcgtttc taaggtcttt tcagacaaat taagcggtca
      481 atcggtcgaa cgcccacaat tacaagctat gcttaactat attcgtgaag gggatatagt
      541 tgttgttact gaattagatc gattaggacg aaataataaa gaattaacag aattgatgaa
      601 tcaaattcaa attaaggggg caaccctgga agtcttaaat ttaccctcaa tgaatggtat
      661 tgaagatgaa aatttaagac ggctgattaa taatttagtg attgaattgt ataagtacca
      721 agcggaatct gaacgcaaac gaattaaaga acgccaagcc caaggaattg aaattgctaa
      781 gaaaaaagga aaattcaaag ggcgacaacc taaattcaaa gaaaatgatc cacgtttaca
      841 acacgctttc gatttatttt tgaatggttg ttccgataaa gaagttgaag aacaaactgg
      901 aattaatcgc cgaacgttta gaaggtatcg atcaagatac aacgtgacag tagatcaaag
      961 aaaaaaacaa tgaaaagagg gatagttaat gagtacggtt attttagctg aaaaaccaag
     1021 ccaggcatta gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg gttattttga
     1081 gatcaaagac ccactattta cagatgaaac gtttatcacc tttggttttg ggcatttagt
     1141 cgaattagca gaaccaggtc attataacga aaagtggcag aactggaaac ttgaatcttt
     1201 gccgattttt cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa
     1261 aattgttgct gaacttctca aaaaggcaaa tacaattatt gtcgcaacag atagcgacag
     1321 agaaggtgaa aatatcgcct ggtcgattat ccataaagca aatgcctttt ctaaagataa
     1381 aacgtataaa agattatgga tcaatagctt agaaaaagat gtgatccgaa gcggttttca
     1441 aaatttgcaa ccaggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat
     1501 tgccgattgg ttgatcggca tgaacgcaag ccctttatat accttgaatt tacaacaaaa
     1561 aggcgtacaa ggtacatttt cattaggacg tgtacaaaca ccgactttat atctcattta
     1621 tcaacgccag gaagcaatag aaaactttaa aaaagaacct tttttcctga ataattcata
     1681 gttttactaa tagtctttcc taacattgat ttaatagtct ttaaagtatt ctgcttcatc
     1741 acccattagg tgatgaaaaa agaacccctt tctgatttag cggacaaata ctttgaccta
     1801 atctcagaag atataaaaga aaaaataata aaatatccga tttatacttt gtataaagaa
     1861 ctaaagtagt tagcaaatca tttacagcaa atcactatgt tccacatacc attttacata
     1921 cttaccttat ccacgacaca tgttgagata gtatcgaaga taaagaaagt tgctccatgt
     1981 tgtttttgcg agatggggaa atcaaagcca cattgacaac catgatgaat aagcttgctt
     2041 tcagtcataa gctaatccta gaaccacttt tcaagtcagt tagccaaatc gatgaagaaa
     2101 gcgaccgtga aagaatggac gctattgata agctaatgga gcaactattg gaagaacgca
     2161 atacccttat tgcactgatg tccaaaggtt tcctagaacc tgctcttttt aatcaggaac
     2221 gaaatgtttt agatagtgag attaaaaatc ttactaccga aaaaacaaac ctggtaacga
     2281 actctgcgag tggggttttg cgagcaaacg agataaagga cctcattaat tatgtgtcag
     2341 cagataattt taatggtgac tacacggaag aactatttga agaatttgta gtgaacatca
     2401 ttgtaaattc cagggatgag ctgacattca atttgaaatg cggtctttcc ctgaaagaaa
     2461 aggtggtgag ataaatggca tatattccat atggatacaa aattcaagat ggagtggtta
     2521 ctgtcgatga aaaggcagca ggtcaagtaa aggtattctt tgagaaatac atatcaggac
     2581 tatcccttac agtggctggc gaacaggcag gtattgaaaa gacacattct gtgatggggc
     2641 gcattttgaa aaacgtcaac taccttggaa atgatacgta tccagcaatt attgataaag
     2701 agatatttga taaagctgaa gaagttagag ataaacgtgc aaaggattta ggacgagtgg
     2761 tagagcttgc cgctttcacc tctccccctc ccaaagaacg attcaaaatg aaaaaggcag
     2821 ataataagat gccagtggat ccttttgaac gagcagaata cttatacagt ctgatagaaa
     2881 gcgaggaata aagtgacaga gaaaaatata atggttattc ctgctcgtaa aagagtagga
     2941 agtacagccg caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg ccgtgtttct
     3001 acagaaacag aagaacaaaa ttctagctat gaggttcagg tctcacacta tacagagttt
     3061 ataaagaaaa ataatgaatg ggagtttgct ggcatctttg cagatgatgg tatctctggt
     3121 acgaacacta aaaagcgtga cgaatttaat cgcatgattg cagagtgtat ggacggtaac
     3181 atcgacatgg ttattacaaa atccatcagc cgatttgcgc gtaacaccct agactgcctt
     3241 caatacatta gacagctcaa ggataagaac atatcagttt attttgagaa agagaacatc
     3301 aacaccacgg atgccaaagg tgaggttttg ctgactatta tggcatcttt ggcacagcag
     3361 gaaagccaga gcctttcaca aaacgttaag cttgggctac agtaccgata ccaacaagga
     3421 aaggtgcagg tcaaccataa gcgatttatg ggctacacga aagatgaaga tggaaattta
     3481 actattgtcc ccgaagaagc tgagattatc aaacgcatct accgagaata ccttgagggt
     3541 cagagtctag tgggcattgg tcgagccctt gaaaaggatg gtattttaac agcagcggga
     3601 aaaccaagat ggcgaccaga atcagttaag aagatacttc aaaacgaaaa atacatcgga
     3661 gatgcccttc tgcaaaagac tgtcacagta gattttctga ccaagaaacg agtgaaaaat
     3721 gaagggcatg ttccccaata ttatgttgaa aatagccatg aagcgattat tcctaaagag
     3781 ttatttttgc aggcgcaaga agaaattcat cggagaagca atatctacac aggagaaggc
     3841 aagaacaaaa gaatttacag tagcaagtac gctttaagtg ccatcacctt ctgtggagat
     3901 tgtggcgata tttatagacg ggtctattgg aatatccacg gaagaaaaga atttgtctgg
     3961 cgatgcgtga ctagaatcga gcaaggtcct gaagtttgta agaaccgaac cgtaaaagaa
     4021 gatgaacttt atggtgcagt aatgactgcg attaataagc tacttgcagg tgggaacaat
     4081 atgataaaga cactggaaga gaatattcat gctgtgattg gcgaaaccac agaataccaa
     4141 atttcagaga ttaacaactc actagaggaa aaacaaaaag aactaatcaa gctggcgaac
     4201 aagggtcaag actatgacca cttagcagat gagattgatg agctgagaga caagcgacag
     4261 atccttttag tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt
     4321 gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag
     4381 ataatccaga gcgttacagt ctatgaaggc cacttcgtca tagccttcaa atctggcatc
     4441 gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctacagtag agttgtgggt
     4501 tttcttttgc ttgctgatta taataatatt ttaataacta ttgtgtttat caaccaaatg
     4561 gtttataata ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa
     4621 agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac
     4681 tccacagaat ttcaataaaa aattacaacg agaaacagta accttggatg agttgaaggc
     4741 cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagaaga
     4801 aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata
     4861 gtaacagaga aattatcatc tttgcctttt atagaaggca tcgtattagg gggctcacgt
     4921 gcaagaggca cccatacaga aaattctgat atagatatcg gaatctatta caattcagac
     4981 tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga gaatagaaac
     5041 aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg atggttagtt
     5101 ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga acaaataatc
     5161 aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat
     5221 ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa
     5281 agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg
     5341 atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg
     5401 gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa
     5461 gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt
     5521 gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta
     5581 ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa agaagtgaat
     5641 gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa
     5701 atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact
     5761 ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg
     5821 tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc
     5881 ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac
     5941 ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga
     6001 gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt
     6061 aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca
     6121 tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact
     6181 gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga
     6241 aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag
     6301 ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg
     6361 gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta
     6421 ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat
     6481 ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca
     6541 cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta
     6601 agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata
     6661 atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat
     6721 cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta
     6781 gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt
     6841 aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag
     6901 tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc
     6961 cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg
     7021 agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac
     7081 tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac
     7141 cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg
     7201 gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat
     7261 ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat
     7321 agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag
     7381 ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac
     7441 cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa
     7501 aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg
     7561 acaatactga taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta
     7621 aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt
     7681 tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata
     7741 aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct
     7801 atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     7861 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     7921 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     7981 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     8041 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     8101 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     8161 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     8221 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     8281 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     8341 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     8401 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     8461 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     8521 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     8581 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     8641 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     8701 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     8761 ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa aagctttaac
     8821 tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc catactctga
     8881 tgttttatat cttttctaaa agttcgctag atagagttct atattagaga tgtaaagagt
     8941 tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag aagcactaac
     9001 agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga ggaggaataa
     9061 aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc aaaaaaaata
     9121 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     9181 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     9241 cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt gggtgcattg
     9301 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     9361 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     9421 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     9481 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     9541 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     9601 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     9661 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     9721 tggaacaaat attaaaacaa atgctttcat ataaaaaact atatgcccta caatgccgtt
     9781 gtaaaaattg gggaaaaggg atatattgat tttatatctt tctcaaaata aatataaaag
     9841 ccctttaaag agggctttta tatattaatc acaaatcact tatcacaaat cacaagtgat
     9901 taatcacaaa tcacttgtga t
//
LOCUS       DAZUJO010000252         9169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.105, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000252 DAZUJO010000000
VERSION     DAZUJO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.105"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1014)
                     /locus_tag="ACG8OL_003123"
     CDS             complement(73..1014)
                     /locus_tag="ACG8OL_003123"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS4818997.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1140..2261
                     /locus_tag="ACG8OL_003124"
     CDS             1140..2261
                     /locus_tag="ACG8OL_003124"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS4818998.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2519..4021
                     /gene="eat(A)"
                     /locus_tag="ACG8OL_003125"
     CDS             2519..4021
                     /gene="eat(A)"
                     /locus_tag="ACG8OL_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS4818999.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4088..4615
                     /locus_tag="ACG8OL_003126"
     CDS             4088..4615
                     /locus_tag="ACG8OL_003126"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS4819000.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            4826..5887
                     /locus_tag="ACG8OL_003127"
     CDS             4826..5887
                     /locus_tag="ACG8OL_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS4819001.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5890..6786
                     /gene="murQ"
                     /locus_tag="ACG8OL_003128"
     CDS             5890..6786
                     /gene="murQ"
                     /locus_tag="ACG8OL_003128"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS4819002.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            6800..8782
                     /locus_tag="ACG8OL_003129"
     CDS             6800..8782
                     /locus_tag="ACG8OL_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS4819003.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(8847..>9169)
                     /locus_tag="ACG8OL_003130"
     CDS             complement(8847..>9169)
                     /locus_tag="ACG8OL_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS4819004.1"
                     /translation="ENSDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEH
                     EDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILA
                     ENNK"
BASE COUNT         2989 a         1640 c         2013 g         2527 t
ORIGIN      
        1 tttttgatct tttattgggc ttaaaaaaag agaaccagct gctgattcag ctgattctca
       61 tcagagagcc ttttattctg cgccaaggat tccttctttt gcatggacta attgggtcaa
      121 aaaggcacaa tatggtgtag caatatcata tttttgtcct tttcgccaaa tggcaccatt
      181 gatgtagtcg atttcagtca aacgatggtt tttgatcagg tcttggtaca tagatgggta
      241 atgcaacccg atgttgtcag gatcataagt agaagcaata tgttggtaca cttcttcttg
      301 atctagtatg attccttctt ttgctgcgat agaagcaaat tcagatacga ttgttcgaac
      361 catgctctct gccgcttttt gcgcgcctag ttcagcaata ttacaatcca aaatcgtgca
      421 taaaccattc aatgttccat ttacacaagc ttttcgccaa atggaataac gaacgttatg
      481 gctatagatc ggatttaaat ttgcttcttg aaagacttct acaacttttt ttgtaaatgc
      541 ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaaca atgtgacttt
      601 tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat ttttctttgg
      661 cacatatttt tccagtacat cttcatgacc taatccattt agtaaacaca aaacataagt
      721 attgtcagta ataatggatt ggatcgaaca aaacatatca tctaattgat tggcttttgt
      781 taatgctacg atcaagtcca cgtgttcgtt gctttcgatg atttcttctg gtgaatagat
      841 tggcagtttt gcgaccactt ctttaccgtt aaagtcagca atcaacccat ttgtacgaat
      901 ggcttcaatg tgagcaggcc aacgatcgat caagaggacc tcatttccgc tttgatgtag
      961 catcaatccg attcggctgc ccattgctcc ggccccagct attgcgattt tcatgttttt
     1021 tctctccttt attcaatgag ttccctttta gtatatagaa aaatggaaga aaatagcatt
     1081 gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga gggatacaga
     1141 tggatatttt ggcggcaatt gattcattta aaggaagtgc ttcttcattt gaactaaatc
     1201 aggcagttct aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg ccgattgcag
     1261 atggcggaga aaatactgca gaagcaatct attacgcgct caggggaaca tggcgtacca
     1321 ttgatgggat ggatctctta ttccgtcagc gaaaaatcag ctatttattg acaacttggc
     1381 aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt ggggttagat ctacttgata
     1441 aaccaacgga tcaaactatc cgacttgctt ccagttatgc cttaggagaa cttattaaag
     1501 atgcactgct tcaagaagta gaacaaatta ttgtcacttt aggaggaagc gggtgtagcg
     1561 atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac acagaaggaa
     1621 atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt gtttctaaag
     1681 taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa ggagctgctt
     1741 ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta gataaacaag
     1801 caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat gatattccag
     1861 gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg aagatggttt
     1921 ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa caagcagatg
     1981 tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa gttccatacg
     2041 gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc ggaagtagag
     2101 accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt catttgggtc
     2161 caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca aaaatcttag
     2221 gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa tcattttctt
     2281 tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg aaaaattaag
     2341 tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc agtgtgcaga
     2401 ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat ccaacccttg
     2461 catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt gatggaaaat
     2521 gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca cattattatt
     2581 tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg gacgaaacgg
     2641 tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc aagggcaagt
     2701 aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag aacgtttaac
     2761 ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa gagagctcca
     2821 attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat cggggggaga
     2881 gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc cgttaatcga
     2941 tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt atttgaaaaa
     3001 gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg aagtagtgga
     3061 ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt tctctatcta
     3121 tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa
     3181 agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga
     3241 aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac gagtgaataa
     3301 aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg
     3361 gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat atatcgattc
     3421 tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag aagatcttca
     3481 attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc ctcatcagcg
     3541 ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt atcttctggg
     3601 ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga gcattagtta
     3661 tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag agaaaaacca
     3721 agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa gagatgtttt
     3781 tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat tggctaaatc
     3841 tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt tggatgtctt
     3901 caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt tactagtgga
     3961 acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg agagaatcta
     4021 acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca acgaaggaga
     4081 gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac aaggattagt
     4141 ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac agaaactagc
     4201 agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc ctcttcaacg
     4261 agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga ccgttgatga
     4321 acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag atgagaactt
     4381 tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag tcttagatgt
     4441 gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa atgtctatct
     4501 gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc cgatgcctaa
     4561 tgaggggttc tttacattca tggtagataa tacagaattg gtttcttatg agtaattatg
     4621 tttttgtgtt atttaaataa aatatttcaa ggcactgctt ttgcttagca gtgccttttt
     4681 taggttatga caattctttt gataatataa actcaacctt tagaaatgaa atttaattat
     4741 tttaataaaa gtattgaaat tttgtttctt tagatatata ctctagtcat agaaatgaag
     4801 acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga acgattcttt
     4861 gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg tcggtatttt
     4921 cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat tgatcgaact
     4981 aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag gagaggcatt
     5041 ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg gtgtaacagg
     5101 tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac atcaattgaa
     5161 aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga aagaagcaga
     5221 agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg aaacagggct
     5281 ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc tccttgtcca
     5341 aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat tgccaacttt
     5401 ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag caagtaacac
     5461 ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc aatttgcttc
     5521 tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag aattttacga
     5581 atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca tacgaagcgt
     5641 agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta cgcgtatgaa
     5701 agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga tccaattaac
     5761 caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga
     5821 gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa gaaaagaagg
     5881 aagataaaaa tgagcaaaat caatttagag aacctaacaa cggaacgcag aaaccaagaa
     5941 acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa caaagaagat
     6001 cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac
     6061 acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg aacaagtgga
     6121 agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga accggaaatg
     6181 gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga aggggccgaa
     6241 gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa aaatgatata
     6301 gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt agattacgca
     6361 aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga aatcagtaaa
     6421 cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg ctctactcga
     6481 ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat cagtatgatt
     6541 ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta
     6601 atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga aacagctgaa
     6661 aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat
     6721 agtgataaag aggaagctca agcaaaatta gctgaagcaa acggatttgt aaaagggacc
     6781 ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca
     6841 aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact gcatgactcg
     6901 tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa aagcgattcc
     6961 aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac ctgggaaagt
     7021 gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag aaaaactttc
     7081 ttctgtaagt acagctacag caaattcatc agcatctgga aaagaccgtg taaacgaacg
     7141 ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt
     7201 attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg gtactgggat
     7261 cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac ttgatgcagc
     7321 aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt ttagctacct
     7381 agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa ctcttggtgg
     7441 agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac tgaaaaactt
     7501 atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca tcttcgctgt
     7561 ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg cggtagatat
     7621 catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt tcttcatcat
     7681 gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg tacttggcgt
     7741 tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg ttatgttcgg
     7801 actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg gtcgtacgct
     7861 attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat tggcattatg
     7921 gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt taccagtcgg
     7981 gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag gacgcccatt
     8041 tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga tcggaaatgt
     8101 cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct ctgacggtaa
     8161 atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg ttgctacatt
     8221 cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg aaacagccac
     8281 agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg tgactatcct
     8341 ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc cagtttttgc
     8401 acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata tctattctcc
     8461 agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga tcacaacgcc
     8521 aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag gcggtacgcc
     8581 atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg tagcagaagc
     8641 tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa
     8701 tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc
     8761 tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa gaaactgaaa
     8821 tctatttttc agcttcttct tttttattat ttattgtttt ctgctaaaat ctgataaatt
     8881 cgtgtcacat cagatggtgt tccccgtaat tcataatctg ccaagaaagg aaagccaaat
     8941 gtttgtgcat attgtttggc tgttagacaa tactgattat tgaagttttt atttccgcta
     9001 ccgactacac caagacaaag tttatgattg tcttcatgtt ccagatattc acgcatcgtt
     9061 tcggtcaaga tttctgtatc accgttatct aaaccatttc cgccgtctaa ataagtagga
     9121 acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac tattttcat
//
LOCUS       DAZUJO010000253         7958 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.113, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000253 DAZUJO010000000
VERSION     DAZUJO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.113"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACG8OL_003131"
     CDS             128..1564
                     /locus_tag="ACG8OL_003131"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS4819005.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACG8OL_003132"
     CDS             1753..4200
                     /locus_tag="ACG8OL_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS4819006.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8OL_003133"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACG8OL_003133"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS4819007.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACG8OL_003134"
     CDS             5487..5693
                     /locus_tag="ACG8OL_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4819008.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACG8OL_003135"
     CDS             complement(5690..6793)
                     /locus_tag="ACG8OL_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS4819009.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACG8OL_003136"
     CDS             7345..7893
                     /gene="aac(6')"
                     /locus_tag="ACG8OL_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS4819010.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2205 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taatttttta ttttataagt aagataaata atgaaagaat agagatactg
     7261 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
     7321 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
     7381 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
     7441 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
     7501 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
     7561 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
     7621 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
     7681 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
     7741 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
     7801 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
     7861 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
     7921 agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DAZUJO010000254         5955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.136, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000254 DAZUJO010000000
VERSION     DAZUJO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.136"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            474..938
                     /locus_tag="ACG8OL_003137"
     CDS             474..938
                     /locus_tag="ACG8OL_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS4819011.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            922..1551
                     /locus_tag="ACG8OL_003138"
     CDS             922..1551
                     /locus_tag="ACG8OL_003138"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS4819012.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1905..3383
                     /gene="msr(C)"
                     /locus_tag="ACG8OL_003139"
     CDS             1905..3383
                     /gene="msr(C)"
                     /locus_tag="ACG8OL_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS4819013.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3430..3876
                     /locus_tag="ACG8OL_003140"
     CDS             3430..3876
                     /locus_tag="ACG8OL_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS4819014.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(4075..4590)
                     /locus_tag="ACG8OL_003141"
     CDS             complement(4075..4590)
                     /locus_tag="ACG8OL_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS4819015.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4707..5909
                     /locus_tag="ACG8OL_003142"
     CDS             4707..5909
                     /locus_tag="ACG8OL_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS4819016.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1844 a          996 c         1255 g         1860 t
ORIGIN      
        1 aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa aaacagattt tcatgttttt
       61 tgtttggttg aacttattac cgcagatgca agtttttgaa caccgtttac tcaaaaaagg
      121 ttgtgacaac atcttgtcat aactttttta ttactgttta agcatactga tccaatttct
      181 aatattcgac gggattcttg caaaaggcga tttcggaaat ttttggcggt gtaaaatttt
      241 ctactgcaga agagctatct tctatagttt gataagaatc aataatcttg gttttagcgt
      301 tgtgctttct ttatcatttt ttccgttcat tctacaaaaa atttcttttc tctatacttt
      361 tctattgaga gcgttttttt tattacacag ctgattcttt gacttttttc tttttttatt
      421 ctattattga caagtcaatc gttgacgagt caattattag aaaggaggaa attatgtctt
      481 tagaagagat tagtcacctt ttatatcaaa tcaaattagc cgatcaaaaa atcgtccaga
      541 tctttgaaga aaatatcggt ataagtctta ctagatacga gatcatgatg agactgaaag
      601 aagaaaatcc attgctacaa agcagtttgc aagaaaagtt gcagattgat caaggagcag
      661 ttactcgtca tctgaaggta ttggaagcaa atggctatgt ttcgcgtcag agaaatcctc
      721 agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc ccagaagaaa atcgagcatt
      781 gtgaaatgga ccataaagat acaggttccg tgctatcccc ggctttcagc gatgtagaaa
      841 tgaaacaatt tctaacactt ttagataaat tgaataccca actttgcaat ataacaacag
      901 aaacgaaaga ggttaataat aatggaacaa aaaaatgatt tttcaacaat tatgactggt
      961 agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga aatgctggaa
     1021 atgatcaaag aagcgacgac tgcaccttct tctgtcaata tgcaaccatg gcgctttgtg
     1081 gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt caatacgaga
     1141 caaaatgata cttcttctgc aatggtcatg atttttggcg atatgcaatg ttacgaatat
     1201 ggcgaagaaa tctataacca agcttacgaa tcagggaaga tgccaaaaga agtcagagat
     1261 caacaattag ctgcgatcat tccttattac aagagttttt caagagaaga aatgaacgat
     1321 gtagtcaaag tcgattctag cttagcggct atgcaattca tgttagttgc gcgtgatcat
     1381 ggctatgaaa caaatccaat tggtggagtc gaagcagatc agttggcaga ggcatttggt
     1441 ttggataaag accgttatgt tccagttatt attttatcaa taggaaaagc ggtagaagaa
     1501 ggttacgagt ctgttcgttt agcaccagaa aagatcacta cgttcgaata aaaggttttc
     1561 agttactatt cagtagctat tgcttttttt gaatcgtgtg tggtaaccta ataaaaaagg
     1621 aggtcggaaa aatgactgca tcgatgaaat tacgtttcga acttttgaat aacaactaaa
     1681 taaattcaaa agacatgtat aaacaacaat ttcatggatg gagtatgtcc attttttcga
     1741 acgacagtag aataggtgaa agctatgaca gatttggtga tttagtcata gttatttcgt
     1801 tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca gatttttctg ctctttttta
     1861 gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa acatatggaa aatttagcag
     1921 taaatataac aaatctacaa gtcagctttg gcaaccagct agaactatct attgattctc
     1981 ttcgtgtcta tcagcaagat cgtataggga tcataggaga aaatggtgtc ggtaaatcga
     2041 ctttgctcaa actaatagcc ggtgaacttt ttcccgatca tgggaaaatc caaacagaga
     2101 tcaccttcaa ctacctgcct caattaacct atctcgctga agcaaaggac ctaaatttgg
     2161 aattagccag tcatttccag ttaagactgg aagaaacttc agagcggaaa tggagcggag
     2221 gggaagaacg aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa gggatgcttc
     2281 tagacgagcc aacaacccat ctagatagaa aaagtattga tcgactgatt gaagagcttc
     2341 gttattatta tggtacgctg gtttttgtta gtcatgatcg ctattttcta gatgagctag
     2401 catcgaaaat ctgggaagta aaagacggag aaatccgaga gttttcgggg aattatagtg
     2461 cctatctcac tcaaaaggaa ttggagaaaa agactcagct acgagaagca gagtcgatca
     2521 tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa gcggaaaagt
     2581 tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag accggatcgg ttgtcttcct
     2641 ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca ttagagagaa
     2701 gactccaaaa aataggagaa acaaccaagc cgcagcagat gaaacaaatc cgttttccag
     2761 taccaaaatc tcttgaactc cacagccgct atcccatcat gggacaaaat gtccaattgg
     2821 aaagaagtgg gagaacatta ctggtaaatg gtgattttca gttttcttta ggtaaaaaaa
     2881 tcgcgattgt cggtgaaaat ggttcaggta agacaacctt attagaacat atccgcaaac
     2941 aaggagaagg aatccttctc tctccgaaag taagctttca agtatatcag caaaagggtt
     3001 atcaaatgac atctgaagaa tccatcattc gttttgtcat gagacaaaca gagttttcag
     3061 aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc tcaggaaact ttgacgaaac
     3121 cgttatgtac attaagtggg ggagaagcga cccgtctgac gattgctttg ctttttacta
     3181 agccaagtaa tgtgttgctg ttagatgaac cgactaattt tattgatatg gcaacgatcg
     3241 aagctttaga gaagctaatg caaatatatc cgggaacgat tttgtttact tctcatgatt
     3301 cttactttgt cgagcgtacg gctgatgaag tttatgaaat aaaagggcag aaaataaaaa
     3361 aagtacttac gagaaatttt taaatgtgct acgatgattt tcgtgacaaa tttaaaggag
     3421 tggtacagat tgcctacaaa taaaaaagaa ggaataattt ttacaacaat gatgtgtttt
     3481 ttgatggtct tggggatgag cgcgtataat ttatggctgc atcaagatct tcatttatca
     3541 agtttgttga ttggtctagt gcctggattc gtggtggctt tcattttgga tgtcttcatc
     3601 atcggtgtgc tggcgaaaaa aattgccttt aaactaccag taaatccaga aagcaaggtc
     3661 caaatgatct taactatttc atgcttgatg gttttaggaa tggtgacatg tatgtcttta
     3721 ttcggtgtga tcatggaaca aggaatacct gatcaattat tctctgctta tatgaatgct
     3781 tggaaaatga atgtgattgc cgctttaccg ttgcagttga tcatcgttgg cccgctttca
     3841 agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga acactgttat ttgaaaaaag
     3901 gccttggcat gctttttgtc atagtctctt gaatgcagtt ctagaaaaat agtgatccga
     3961 acgataggtt ttggcctgtc gttcggatcg ctttttcttt tccaactgta ttgctctttt
     4021 attgaagata atttgctctt caatagatat ttctagaaag gttctgttta tcccttactc
     4081 aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat tggagttcct gctgacaaag
     4141 cataattctg cgctggatca caacataacg tccaaattct tgtttcttgt ctagcgagag
     4201 gtcattcaat tttttttgat ttttttgcat gattttgttc agctgttgtt ctctagtgaa
     4261 tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt ttgctagaaa
     4321 ttcatctttt tgcgtaggag cgggagtttc ctctttgatc cattcctcta caagagcacg
     4381 tccttcatca gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt ccaaaacggt
     4441 cacataaccc tgctggtcta acttggcaag cgttgtatag atctgactgt gatgggcttc
     4501 ccaaaaaatt gtgatcagct gcttcaattc atacccagaa cgtggtttgg ataaaagcac
     4561 gcataataat atataggcta aagtattcat aaaaatccta cctctcgtct tattttcttt
     4621 attatatcac agtaaagttt ccacgtttta catgtgcata tttacatgta aaataaatca
     4681 tgtatgatgc taatagagag aaagggatgg aaatattgga gaatcaaata gatatacaag
     4741 aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct agttgtagca gctacctgta
     4801 tgatcgcttt tatgggagtg ggtctagtag acccaatctt aaaaacgatt gctgcacagt
     4861 taaacgctac gccagctgaa acgaccctgt tatttactag ttacatgtta gtcactggtg
     4921 tggtgatgct atttacaggt ttcttatcta gtcggatcgg tttgaaaaaa acattgatga
     4981 caggattgtt tatcatcgtt gtattcgctg gtcttggcgg attatctggg aatatcggaa
     5041 tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc tctactgctt
     5101 tagcaacgat cgtcagtatt ttagttggta acacagaaaa agcaatcatg atgtatgaag
     5161 cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg ggcagtggtt
     5221 catggcgtta tccatttttt ggggtagcat ttttgatgtt ttgcggtttt atagccctag
     5281 cggtactttt acctgaaact gagaaaccaa agagaaaagt aggcctttgg gaaggtgtca
     5341 aagcgttaag caatcataaa cttcgttcag ttggactaat cgcattgtta tataatttcg
     5401 gattctttac tttacttgcg tatgcgccat ttttattgat tggctattct gaattagaag
     5461 tcggttttgt attttttggc tggggtgtgc ttctggctat tgcttcgatt ttcgttgcac
     5521 cggctcttga gcggaagttt acgacttatc ggacaatttt agcagcattt gtcttattca
     5581 ttatttgttt gctgttatta ggagctggaa cttctatgcc agtccttgta ccgatcagca
     5641 ttgtgttagc aggatttttc caaggaatta tcaatacact gttgacgacg attgctatgg
     5701 aaataccagg tctgcaacga aacgttgctt cgtccagcta tagttttgtc cgctttttcg
     5761 gtggagcttt agcaccattt attgcaggtg tgattggtga acatattggt gaaagctatg
     5821 cgttttattt tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt tatcaccgta
     5881 attattttgc tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa gctgtcctgc
     5941 atcaagtcat aagaa
//
LOCUS       DAZUJO010000255         4722 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.151, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000255 DAZUJO010000000
VERSION     DAZUJO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.151"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..709
                     /gene="dfrG"
                     /locus_tag="ACG8OL_003143"
     CDS             212..709
                     /gene="dfrG"
                     /locus_tag="ACG8OL_003143"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS4819017.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(781..2733)
                     /locus_tag="ACG8OL_003144"
     CDS             complement(781..2733)
                     /locus_tag="ACG8OL_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS4819018.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2740..2976)
                     /locus_tag="ACG8OL_003145"
     CDS             complement(2740..2976)
                     /locus_tag="ACG8OL_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4819019.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3378..3668
                     /locus_tag="ACG8OL_003146"
     CDS             3378..3668
                     /locus_tag="ACG8OL_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS4819020.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(3940..4289)
                     /locus_tag="ACG8OL_003147"
                     /pseudo
     CDS             complement(3940..4289)
                     /locus_tag="ACG8OL_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4382..4684
                     /locus_tag="ACG8OL_003148"
                     /pseudo
     CDS             4382..4684
                     /locus_tag="ACG8OL_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1516 a          765 c          823 g         1618 t
ORIGIN      
        1 ttttaactta cataccaaat tacatacaaa atgatttata aaatgcaatt gaaaatgata
       61 attgaatgaa aatttgtgat tttgtggtat tatgataata taacttatag aaaaaattgc
      121 ttattcaact aacgggcagg ataatgagaa aaggtttttt gatagaaata agaaattaga
      181 tggtaaatta gttaaaaagg ctgtgttata aatgaaagtt tctttgattg ctgcgatgga
      241 taagaataga gtgattggca aagagaatga cattccttgg aggattccca aggactggga
      301 atatgttaaa aatactacaa agggacatcc gataatatta ggtaggaaga accttgaatc
      361 aatcggaaga gccttacctg acagaagaaa tattattctg acgagagata aggggtttac
      421 ctttaatggt tgtgaaattg ttcattcaat agaagatgtt tttgagttat gtaaaaacga
      481 agaagaaatt tttattttcg gaggagaaca gatttataat ttgtttttcc cttatgttga
      541 gaaaatgtac atcacaaaaa tacatcatga attcgaagga gatacttttt ttccagaagt
      601 gaattatgag gaatggaatg aggtatttgc ccaaaaaggg ataaagaatg ataaaaatcc
      661 gtataactac tattttcatg tatatgaaag aaaaaactta ttgagttaac gggtgcgtta
      721 attgaagaag aacagtaaag ataaagcaca agagcttggg attactccaa gctctttttg
      781 tcatttgaaa taaattatat ctttccaatc aaattgttta tctgtaatcc ctaatctttg
      841 agcaggtgtc aaaacctttt tatccgctga tttaaaaggg cgacaaaagt tgtagtaagt
      901 tcttagaatt gtaatcgcaa attgagcata cttcggattg aagttagcgt aaatatagct
      961 cttcttatca ccacgagctg ttgttaatgg tctttcaaga gatgatatat aacgtcttat
     1021 ctgttgaata aagctattgg tcgaatggtc attaacattt aatagcatct ttgcgatttc
     1081 gtttggttcc agagcagata aatccgttct acaatcaact gaataaaatc ctttatcctt
     1141 agtggctaat ggatgtttga tagggttatc ggcatatttg cgatatcttt ctccatcttt
     1201 actaatagcc tcttcgtgga attgatgact tgttagtaat tcagttagat aatgagatgc
     1261 caaagtccgt agaaactttg ttttgaatcc tctgatatct ccccaatcga gcagttctgc
     1321 ttttgcttgt ccgaattctt ttaagcattg ttttctgctt ttcgtcttgt taacttggca
     1381 aataaaatga tgtgcatcgc tcaaccttaa ttctttcgtg aatactctat aaaaggcgtt
     1441 tctaatagag aagtcatcat cactaatcat tcgccattcg tcggcattaa ccatttgttt
     1501 aattaaccaa tagtgagcag ttgtagtata aggagcatca acatttaaac catcgacaaa
     1561 tctactccta ttagtaattt tccctaattc gtgaagataa gcattcctat tttcgctatc
     1621 gttagcggat ggttcttgtg gataataaga ccaatctaac ctatcatttt tacgactaaa
     1681 agtattgaga tggtcttctt tgaattttct tgtatcttca tttaattcat ccattgaaat
     1741 attccaatca taggcaacat cagaacggaa cacatatctt gataagacct cagcagatac
     1801 aactatatac gtttgcatat taaggtcttc aaaccccgta tatttctttg aaccttgtcc
     1861 tttcttacgg acgttattta ggtagtagtg catcttgtcc gtattaagcc acatctcgtt
     1921 aaatttcttt gtttgaaggg gttgtgtctc atatcgttct aaaaactcca aacaacgcct
     1981 atacagccac tccagcttat gatagtaggt tccaactcca attcctaaaa tatcacaagt
     2041 acgactgact gaaacccttc caacaaccat cttagcgaac attggaagaa tggtgttctt
     2101 ttgttggtga taagtagtgg tttcttctct ttttggtaga acattagtga acttcttaca
     2161 cgccttacat tgatagcgtt gagatttgcc cctacttttc ccccttttgt aaaattgctt
     2221 cggctcattg aatggggttg attcttcttc cgagcagcct tctttatgaa attgataatc
     2281 tggctcaaca tcttggatgc tattgatttc tataaggcgt ttaatttctt caacaacaga
     2341 ccaattagat aaaggggtta ctgtacagtt ttgcgataca cctctgccaa taggattgtc
     2401 attgcagtac atacctttat gacctttatc tttggaacta ccagtcattt tatatcgact
     2461 tggtttccct tttacatctt tgaatttgtg ctgttctttc ccaaaattac aacaaaacgg
     2521 attgatgcag taattatatt gaatgggata ttcagaaccg ttccatttaa aagacgttgg
     2581 caagaacaac attttgttat accttgttgc aaatcgtttt ggagttaata aatcatattg
     2641 tttagcccta tcttttaatt cttcgtttga aatcggaatt ttaacatcta caatttcatc
     2701 ttccttcgtg gctaatcttt taagcttggt cattgtctat caacccactc tatctttgat
     2761 acccattttt tcaacaatcc gcttattact tcgcttgttg gcaatccatt caataaactt
     2821 cttatcgtct aaaatgttca ataagaattc atccactgct tcgctttcag tacgctctgc
     2881 atattcagcg tattgcttaa ctatcaccct tacttgctct gatatttccc aatccacaga
     2941 cttagcgttc ttattttttg gctcaagaaa ttgcatagga aaatgcaccc ctttttgctt
     3001 agtaaattgt aaccatttta acacaccaat ttgcattcct acaataatta tttcacgttc
     3061 gcatttttat atacatttta acttacattt tgtggtgtaa attaacaaca aacgttgatt
     3121 taacaataaa aaacgagtat caaattcagt tgcaatttga tactcattta ggtttacatt
     3181 ttcacttttt gtactccaaa actgaactac ttaaatctat actgctgcct ctttttttta
     3241 gtacatgtcc ttcatcttcg caggaagtgc caatatgatt ttaagaaagg taatagacaa
     3301 aaagtcaaaa agataaaaca gagtttcttc tttacaaagg gaatgtatgt tcgtataatg
     3361 gtattgaggt gaaggttatg gagaaaaaat atggaacagt agataagatc cgtactttgc
     3421 aagttagtgc cagaccgttg atccgtttca gtttaaatgg ggtcaattgt ttgattgctt
     3481 cccatagttt gaacttccta gctgaagtag acgaagggat gaagttagtc gttacaggtt
     3541 attataataa tagaaagcaa tttgttgtga gatcctatca tgtgttaggg aaacccaaaa
     3601 ttgtggtaga atatgagaaa agtctatacc caagaaaaaa ggtccgacaa gatgaaagaa
     3661 taaactagaa aacaagcggc tgagacaaaa atgtcctagc cgcttgtttt ggaaagatgg
     3721 atgcagacga tgggatttga acccacacga gcatacgctc acaagaacct caatctatat
     3781 ataactacat gtatttaagt atttgtgatg aatcactaaa ctttccaaac tcatttgagg
     3841 gatattgctt aaagattgaa cagatcaatt cgaagcccta ttagtattcg ctcaatgggg
     3901 ggatcagagg ccataaaaag agactagtca ttactagtct caggtagtgg aattgtagga
     3961 ttttaaacgt tcctggagag taccggtatg taactcgaag aggtgattat catagtcgta
     4021 aaagtaaatt gaagaagcct cgcctttcac acgagaacgt cctggttcga cagttaaacc
     4081 taacatttgg atttttgaga caaacgaatc tatatcttgt tcgtcaattt taaaagcaat
     4141 atgattgtat gtctttggca gtttattcga cgaattctgc attactgcaa tccataggtc
     4201 cttaactaag aaaaattttt ctggatataa tgaaaatttt tttgttgact atcgtagatt
     4261 tcttttgcat caaataaggt atttaacaat taagtattta aaagataact tttaagttat
     4321 ctttaagtaa tgcttaaagc tatgttatac tctattagga aatataagaa ggagtataaa
     4381 aatgaacaaa cataatagaa aaataacgaa taacaaagaa ctgatcttgt tactaatagg
     4441 gatttggggt atctttttac tctttaatat gtatatgccg actatgagag cggatgacgt
     4501 agtttatgca agtagattag atgaacttgg ctatttgggt gcttcgatag aacattataa
     4561 gacatggagt tcaagaatta ttatagaact attcttaatg ttcttttcta aacactttat
     4621 gctatggaaa ttattaaatt ctacgattat gttaggaatt gtagtattac tttgcaagta
     4681 tgttgagagt ataaaatatt ttgtgtaaat gaaaaaatcc at
//
LOCUS       DAZUJO010000256         2503 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.177, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000256 DAZUJO010000000
VERSION     DAZUJO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.177"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..787
                     /gene="erm(B)"
                     /locus_tag="ACG8OL_003149"
     CDS             50..787
                     /gene="erm(B)"
                     /locus_tag="ACG8OL_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS4819021.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1050..1913)
                     /locus_tag="ACG8OL_003150"
     CDS             complement(1050..1913)
                     /locus_tag="ACG8OL_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS4819022.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(1915..2187)
                     /locus_tag="ACG8OL_003151"
     CDS             complement(1915..2187)
                     /locus_tag="ACG8OL_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS4819023.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2204..2413)
                     /locus_tag="ACG8OL_003152"
     CDS             complement(2204..2413)
                     /locus_tag="ACG8OL_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS4819024.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          741 a          454 c          402 g          906 t
ORIGIN      
        1 tttaacttct gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa
       61 tataaaatat tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca
      121 attgaattta aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac
      181 gacgaaactg gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt
      241 caacttatcg tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat
      301 tctacagttt caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca
      361 tttaagcaca caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct
      421 gattgttgaa gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct
      481 cttgcacact caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc
      541 taaaccaaaa gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga
      601 taaatattgg aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca
      661 actgtttact aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag
      721 taccgttact tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag
      781 gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg
      841 tagataaatt attaggtata gggcacctct aataactacc aattaattca cctcaaaatg
      901 aaattagaaa aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta
      961 ggcgctcggg accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt
     1021 tggtaactac tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt
     1081 attggtggtg taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg
     1141 gtttcttggt gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt
     1201 tctttccctg atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa
     1261 ggcgtttcta agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta
     1321 aataagcccg ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca
     1381 tgcgcttgtt ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat
     1441 cgttcaatcg ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt
     1501 tcataacctt tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct
     1561 gttccttcaa tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc
     1621 atgcgattcg aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat
     1681 tcgtcaaaat ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct
     1741 tgtgtttctt caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca
     1801 agtaaaaaag cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc
     1861 ttttctaaac gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc
     1921 cactttctct ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg
     1981 aatagcttgt aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa
     2041 ttgatttaaa agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa
     2101 aacataattt aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc
     2161 aaatgttttt tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta
     2221 aatatttccg tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat
     2281 tctttgctgt ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat
     2341 cccccattat atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc
     2401 ctaaatttcc cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca
     2461 cctatcacaa atcacaagtg atttgtgatt aatcacttgt gat
//
LOCUS       DAZUJO010000257         1357 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5378
            SAMEA115707682-rid22576763.guided.205, whole genome shotgun
            sequence.
ACCESSION   DAZUJO010000257 DAZUJO010000000
VERSION     DAZUJO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707682
            Sequence Read Archive: ERR13197410
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288115.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288115.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:00:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,090
            Genes (coding)                    :: 3,000
            CDSs (with protein)               :: 3,000
            Genes (RNA)                       :: 63
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 34 of 90
            Pseudo Genes (incomplete)         :: 54 of 90
            Pseudo Genes (internal stop)      :: 22 of 90
            Pseudo Genes (multiple problems)  :: 16 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707682-rid22576763.guided.205"
                     /isolate="VRE5378"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1345
                     /locus_tag="ACG8OL_003153"
                     /note="aph(2'')-Ia"
     CDS             <1..1345
                     /locus_tag="ACG8OL_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS4819025.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKGASIILCK"
BASE COUNT          560 a          113 c          233 g          451 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaggg
     1321 agcgtcaata attttgtgta aataactcgt ccttctg
//
